Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06977.t1 | XP_003626041.2 | 85.7 | 175 | 20 | 2 | 50 | 219 | 280 | 454 | 4.40E-77 | 297.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06977.t1 | A4PU36 | 85.7 | 175 | 20 | 2 | 50 | 219 | 280 | 454 | 3.2e-77 | 297.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059272 | MS.gene06977 | 0.81066 | 1.03E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06977.t1 | MTR_7g110390 | 85.714 | 175 | 20 | 2 | 50 | 219 | 280 | 454 | 7.95e-95 | 285 |
| MS.gene06977.t1 | MTR_7g108660 | 78.534 | 191 | 26 | 1 | 29 | 219 | 67 | 242 | 1.02e-89 | 265 |
| MS.gene06977.t1 | MTR_7g111570 | 47.826 | 161 | 79 | 2 | 62 | 217 | 461 | 621 | 5.29e-46 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06977.t1 | AT3G62200 | 46.626 | 163 | 82 | 2 | 62 | 219 | 489 | 651 | 1.14e-42 | 152 |
Find 50 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATCAAATGTCTCATATT+TGG | 0.136266 | 7.3:-6370502 | None:intergenic |
| TTCAGGAAGATCGGCAATTT+TGG | 0.227607 | 7.3:+6370689 | MS.gene06977:CDS |
| CGTGGTTCCCAGACGCAATC+TGG | 0.270910 | 7.3:+6370637 | MS.gene06977:CDS |
| TTAGCCTCTGTAAGTGAAAA+TGG | 0.275804 | 7.3:-6370956 | None:intergenic |
| GGGTGATGTACTTAAGATTT+TGG | 0.277803 | 7.3:+6370873 | MS.gene06977:CDS |
| AATATGAGACATTTGATATT+CGG | 0.334288 | 7.3:+6370505 | MS.gene06977:CDS |
| ATATCAAATGTCTCATATTT+GGG | 0.370068 | 7.3:-6370501 | None:intergenic |
| ATAATTGATTGCATTCGTTA+TGG | 0.373706 | 7.3:+6370474 | MS.gene06977:CDS |
| AAGGTGGTTAAACGAGTCTT+TGG | 0.382873 | 7.3:+6370558 | MS.gene06977:CDS |
| CTGCCACGGCAAAGGAGGCT+TGG | 0.385048 | 7.3:+6369891 | MS.gene06977:CDS |
| ATCCTAACCCAGATTGCGTC+TGG | 0.396295 | 7.3:-6370645 | None:intergenic |
| TTATACCTTGTCATTCTAGA+TGG | 0.412658 | 7.3:+6370923 | MS.gene06977:CDS |
| TGCCACGGCAAAGGAGGCTT+GGG | 0.414090 | 7.3:+6369892 | MS.gene06977:CDS |
| GAATACACTGATGAATGAAA+TGG | 0.416796 | 7.3:+6370431 | MS.gene06977:CDS |
| GTTTCTCATACATCAATTTG+TGG | 0.418720 | 7.3:+6369838 | MS.gene06977:CDS |
| CTGTAAGTGAAAATGGTAAT+CGG | 0.431067 | 7.3:-6370949 | None:intergenic |
| TTGTTGCAAATGAGAATCTA+TGG | 0.442211 | 7.3:+6370598 | MS.gene06977:CDS |
| GCAATCAATTATATTTCCTT+CGG | 0.464377 | 7.3:-6370463 | None:intergenic |
| TTATAATCACAATCAAGAAA+TGG | 0.466378 | 7.3:+6370899 | MS.gene06977:CDS |
| TTGCGTCTGGGAACCACGTA+AGG | 0.473407 | 7.3:-6370632 | None:intergenic |
| TCCCAGACGCAATCTGGGTT+AGG | 0.483088 | 7.3:+6370643 | MS.gene06977:CDS |
| TGCTTCTGCTGCCACGGCAA+AGG | 0.497491 | 7.3:+6369883 | MS.gene06977:CDS |
| TGTCTTGAAGAGCTTGTGTT+GGG | 0.501883 | 7.3:+6370853 | MS.gene06977:CDS |
| TTCTGCTGCCACGGCAAAGG+AGG | 0.510728 | 7.3:+6369886 | MS.gene06977:CDS |
| TGAGAATTTGCAAGGACTTG+TGG | 0.513544 | 7.3:+6370389 | MS.gene06977:CDS |
| GTGTCTTGAAGAGCTTGTGT+TGG | 0.527052 | 7.3:+6370852 | MS.gene06977:CDS |
| GGTGGAAGGCTAAAGGCAAA+TGG | 0.529751 | 7.3:+6370324 | MS.gene06977:intron |
| AGGCAAAGGTGGAAGGCTAA+AGG | 0.536609 | 7.3:+6370317 | MS.gene06977:intron |
| GTGGTTCCCAGACGCAATCT+GGG | 0.539558 | 7.3:+6370638 | MS.gene06977:CDS |
| TGAGACATTTGATATTCGGA+AGG | 0.559594 | 7.3:+6370509 | MS.gene06977:CDS |
| TCAATTATATTTCCTTCGGT+AGG | 0.561721 | 7.3:-6370459 | None:intergenic |
| TCCTAACCCAGATTGCGTCT+GGG | 0.566146 | 7.3:-6370644 | None:intergenic |
| TGCAATTGAGCAACAGAAGG+TGG | 0.573790 | 7.3:+6370542 | MS.gene06977:CDS |
| GAAGATTGCTTCTGCTGCCA+CGG | 0.579634 | 7.3:+6369877 | MS.gene06977:CDS |
| CACGTTGGAGAAGAGTTTCG+CGG | 0.590720 | 7.3:+6369916 | MS.gene06977:CDS |
| GAGACATTTGATATTCGGAA+GGG | 0.590858 | 7.3:+6370510 | MS.gene06977:CDS |
| GTCCCAAGCCTCCTTTGCCG+TGG | 0.593240 | 7.3:-6369894 | None:intergenic |
| CAGAAGCAATCTTCTCAAAG+TGG | 0.595702 | 7.3:-6369868 | None:intergenic |
| TGCGTCTGGGAACCACGTAA+GGG | 0.598064 | 7.3:-6370631 | None:intergenic |
| ACGTTGGAGAAGAGTTTCGC+GGG | 0.598848 | 7.3:+6369917 | MS.gene06977:CDS |
| GTGACAAAGGCAAAGGTGGA+AGG | 0.603122 | 7.3:+6370310 | MS.gene06977:intron |
| ATCTTCCTGAAAATGAAGCA+AGG | 0.618998 | 7.3:-6370677 | None:intergenic |
| TTGGAGAAGAGTTTCGCGGG+AGG | 0.622770 | 7.3:+6369920 | MS.gene06977:CDS |
| TCAACACATGAGAATTTGCA+AGG | 0.634115 | 7.3:+6370381 | MS.gene06977:CDS |
| TTGTGCAATTGAGCAACAGA+AGG | 0.644017 | 7.3:+6370539 | MS.gene06977:CDS |
| GCGTCTGGGAACCACGTAAG+GGG | 0.647796 | 7.3:-6370630 | None:intergenic |
| AAAGGAGGCTTGGGACACGT+TGG | 0.659475 | 7.3:+6369901 | MS.gene06977:CDS |
| GGAAGTGTGAACCCCTTACG+TGG | 0.663822 | 7.3:+6370619 | MS.gene06977:CDS |
| GGCTGCCATCTAGAATGACA+AGG | 0.705174 | 7.3:-6370928 | None:intergenic |
| AAATTGATGTATGAGAAACA+TGG | 0.716313 | 7.3:-6369834 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATATAATAAAAGACAAA+AGG | - | chr7.3:6370794-6370813 | None:intergenic | 15.0% |
| !! | AATATCAAATGTCTCATATT+TGG | - | chr7.3:6370505-6370524 | None:intergenic | 20.0% |
| !! | AATATGAGACATTTGATATT+CGG | + | chr7.3:6370505-6370524 | MS.gene06977:CDS | 20.0% |
| !! | AGATTTAAAGATAATAGAGA+AGG | + | chr7.3:6370092-6370111 | MS.gene06977:intron | 20.0% |
| !! | ATATCAAATGTCTCATATTT+GGG | - | chr7.3:6370504-6370523 | None:intergenic | 20.0% |
| !! | TTATAATCACAATCAAGAAA+TGG | + | chr7.3:6370899-6370918 | MS.gene06977:CDS | 20.0% |
| ! | ATAATTGATTGCATTCGTTA+TGG | + | chr7.3:6370474-6370493 | MS.gene06977:CDS | 25.0% |
| ! | GCAATCAATTATATTTCCTT+CGG | - | chr7.3:6370466-6370485 | None:intergenic | 25.0% |
| !!! | TATTGTTTTTCTTAGTTCCT+TGG | + | chr7.3:6370734-6370753 | MS.gene06977:intron | 25.0% |
| AACAATGTAGCCAAATTTCA+GGG | - | chr7.3:6370132-6370151 | None:intergenic | 30.0% | |
| AACCTTGATGAAATGAAAGT+TGG | + | chr7.3:6370180-6370199 | MS.gene06977:intron | 30.0% | |
| AATTTGAATTGTTGCAGATG+AGG | + | chr7.3:6369989-6370008 | MS.gene06977:intron | 30.0% | |
| CTGTAAGTGAAAATGGTAAT+CGG | - | chr7.3:6370952-6370971 | None:intergenic | 30.0% | |
| GAATACACTGATGAATGAAA+TGG | + | chr7.3:6370431-6370450 | MS.gene06977:CDS | 30.0% | |
| GTTTACTTAACCCTGAAATT+TGG | + | chr7.3:6370119-6370138 | MS.gene06977:intron | 30.0% | |
| GTTTCTCATACATCAATTTG+TGG | + | chr7.3:6369838-6369857 | MS.gene06977:CDS | 30.0% | |
| TAACAAGTAATAAAGCTCCA+AGG | - | chr7.3:6370754-6370773 | None:intergenic | 30.0% | |
| TCAATTATATTTCCTTCGGT+AGG | - | chr7.3:6370462-6370481 | None:intergenic | 30.0% | |
| TTATACCTTGTCATTCTAGA+TGG | + | chr7.3:6370923-6370942 | MS.gene06977:CDS | 30.0% | |
| TTGTTGCAAATGAGAATCTA+TGG | + | chr7.3:6370598-6370617 | MS.gene06977:CDS | 30.0% | |
| TTTCATTTCATCAAGGTTCT+TGG | - | chr7.3:6370178-6370197 | None:intergenic | 30.0% | |
| ! | ATTACCATTTTCACTTACAG+AGG | + | chr7.3:6370952-6370971 | MS.gene06977:CDS | 30.0% |
| AAGAATAAAGGTGGTGACAA+AGG | + | chr7.3:6370297-6370316 | MS.gene06977:intron | 35.0% | |
| ATCTTCCTGAAAATGAAGCA+AGG | - | chr7.3:6370680-6370699 | None:intergenic | 35.0% | |
| ATGAAAGTTGGAGAACTTCA+AGG | + | chr7.3:6370192-6370211 | MS.gene06977:intron | 35.0% | |
| CAACAATGTAGCCAAATTTC+AGG | - | chr7.3:6370133-6370152 | None:intergenic | 35.0% | |
| CTCCAACTTTCATTTCATCA+AGG | - | chr7.3:6370185-6370204 | None:intergenic | 35.0% | |
| GAGACATTTGATATTCGGAA+GGG | + | chr7.3:6370510-6370529 | MS.gene06977:CDS | 35.0% | |
| TCAACACATGAGAATTTGCA+AGG | + | chr7.3:6370381-6370400 | MS.gene06977:CDS | 35.0% | |
| TGAGACATTTGATATTCGGA+AGG | + | chr7.3:6370509-6370528 | MS.gene06977:CDS | 35.0% | |
| TTAGCCTCTGTAAGTGAAAA+TGG | - | chr7.3:6370959-6370978 | None:intergenic | 35.0% | |
| ! | CAATTCCTTGCTTCATTTTC+AGG | + | chr7.3:6370672-6370691 | MS.gene06977:CDS | 35.0% |
| ! | GGGTGATGTACTTAAGATTT+TGG | + | chr7.3:6370873-6370892 | MS.gene06977:CDS | 35.0% |
| !! | TGCTTCATTTTCAGGAAGAT+CGG | + | chr7.3:6370680-6370699 | MS.gene06977:CDS | 35.0% |
| AAGGTGGTTAAACGAGTCTT+TGG | + | chr7.3:6370558-6370577 | MS.gene06977:CDS | 40.0% | |
| AGAACTTCAAGGTTCTTTGG+CGG | + | chr7.3:6370203-6370222 | MS.gene06977:intron | 40.0% | |
| CAGAAGCAATCTTCTCAAAG+TGG | - | chr7.3:6369871-6369890 | None:intergenic | 40.0% | |
| CAGCTAGCAAAGAATAAAGG+TGG | + | chr7.3:6370288-6370307 | MS.gene06977:intron | 40.0% | |
| GAGATCTCTGTTGTCAATAG+CGG | + | chr7.3:6369942-6369961 | MS.gene06977:intron | 40.0% | |
| GCGAACTCAATGAAAAGATG+TGG | + | chr7.3:6370057-6370076 | MS.gene06977:intron | 40.0% | |
| TGAGAATTTGCAAGGACTTG+TGG | + | chr7.3:6370389-6370408 | MS.gene06977:CDS | 40.0% | |
| TGGAGAACTTCAAGGTTCTT+TGG | + | chr7.3:6370200-6370219 | MS.gene06977:intron | 40.0% | |
| TGTCTTGAAGAGCTTGTGTT+GGG | + | chr7.3:6370853-6370872 | MS.gene06977:CDS | 40.0% | |
| TTGTGCAATTGAGCAACAGA+AGG | + | chr7.3:6370539-6370558 | MS.gene06977:CDS | 40.0% | |
| ! | GAAATGGTTTTTCCTACCGA+AGG | + | chr7.3:6370447-6370466 | MS.gene06977:CDS | 40.0% |
| ! | TTCAGGAAGATCGGCAATTT+TGG | + | chr7.3:6370689-6370708 | MS.gene06977:CDS | 40.0% |
| !! | CAATTTTGGCGTCTTGTAAC+AGG | + | chr7.3:6370703-6370722 | MS.gene06977:CDS | 40.0% |
| AAAGGTGGTGACAAAGGCAA+AGG | + | chr7.3:6370303-6370322 | MS.gene06977:intron | 45.0% | |
| GCGCAGCTAGCAAAGAATAA+AGG | + | chr7.3:6370285-6370304 | MS.gene06977:intron | 45.0% | |
| GTGTCTTGAAGAGCTTGTGT+TGG | + | chr7.3:6370852-6370871 | MS.gene06977:CDS | 45.0% | |
| TGCAATTGAGCAACAGAAGG+TGG | + | chr7.3:6370542-6370561 | MS.gene06977:CDS | 45.0% | |
| AAATTGCCTCACGATAGCGC+CGG | - | chr7.3:6369974-6369993 | None:intergenic | 50.0% | |
| ACGTTGGAGAAGAGTTTCGC+GGG | + | chr7.3:6369917-6369936 | MS.gene06977:CDS | 50.0% | |
| AGGCAAAGGTGGAAGGCTAA+AGG | + | chr7.3:6370317-6370336 | MS.gene06977:intron | 50.0% | |
| ATCCTAACCCAGATTGCGTC+TGG | - | chr7.3:6370648-6370667 | None:intergenic | 50.0% | |
| CACGTTGGAGAAGAGTTTCG+CGG | + | chr7.3:6369916-6369935 | MS.gene06977:CDS | 50.0% | |
| GAAGATTGCTTCTGCTGCCA+CGG | + | chr7.3:6369877-6369896 | MS.gene06977:CDS | 50.0% | |
| GGTGGAAGGCTAAAGGCAAA+TGG | + | chr7.3:6370324-6370343 | MS.gene06977:intron | 50.0% | |
| GTGACAAAGGCAAAGGTGGA+AGG | + | chr7.3:6370310-6370329 | MS.gene06977:intron | 50.0% | |
| TCCTAACCCAGATTGCGTCT+GGG | - | chr7.3:6370647-6370666 | None:intergenic | 50.0% | |
| ! | GGCTGCCATCTAGAATGACA+AGG | - | chr7.3:6370931-6370950 | None:intergenic | 50.0% |
| AAAGGAGGCTTGGGACACGT+TGG | + | chr7.3:6369901-6369920 | MS.gene06977:CDS | 55.0% | |
| CTTTGCTAGCTGCGCTTGCA+AGG | - | chr7.3:6370280-6370299 | None:intergenic | 55.0% | |
| GGTGGTGACAAAGGCAAAGG+TGG | + | chr7.3:6370306-6370325 | MS.gene06977:intron | 55.0% | |
| GTGGTTCCCAGACGCAATCT+GGG | + | chr7.3:6370638-6370657 | MS.gene06977:CDS | 55.0% | |
| TCCCAGACGCAATCTGGGTT+AGG | + | chr7.3:6370643-6370662 | MS.gene06977:CDS | 55.0% | |
| TGCGTCTGGGAACCACGTAA+GGG | - | chr7.3:6370634-6370653 | None:intergenic | 55.0% | |
| TTGCGTCTGGGAACCACGTA+AGG | - | chr7.3:6370635-6370654 | None:intergenic | 55.0% | |
| TTGGAGAAGAGTTTCGCGGG+AGG | + | chr7.3:6369920-6369939 | MS.gene06977:CDS | 55.0% | |
| TTGTCAATAGCGGCCGCTAC+CGG | + | chr7.3:6369952-6369971 | MS.gene06977:intron | 55.0% | |
| ! | GGAAGTGTGAACCCCTTACG+TGG | + | chr7.3:6370619-6370638 | MS.gene06977:CDS | 55.0% |
| CGTGGTTCCCAGACGCAATC+TGG | + | chr7.3:6370637-6370656 | MS.gene06977:CDS | 60.0% | |
| GCGTCTGGGAACCACGTAAG+GGG | - | chr7.3:6370633-6370652 | None:intergenic | 60.0% | |
| TGCCACGGCAAAGGAGGCTT+GGG | + | chr7.3:6369892-6369911 | MS.gene06977:CDS | 60.0% | |
| TGCTTCTGCTGCCACGGCAA+AGG | + | chr7.3:6369883-6369902 | MS.gene06977:CDS | 60.0% | |
| TTCTGCTGCCACGGCAAAGG+AGG | + | chr7.3:6369886-6369905 | MS.gene06977:CDS | 60.0% | |
| CTGCCACGGCAAAGGAGGCT+TGG | + | chr7.3:6369891-6369910 | MS.gene06977:CDS | 65.0% | |
| GTCCCAAGCCTCCTTTGCCG+TGG | - | chr7.3:6369897-6369916 | None:intergenic | 65.0% | |
| !! | CCTCACGATAGCGCCGGTAG+CGG | - | chr7.3:6369968-6369987 | None:intergenic | 65.0% |
| CCGCTACCGGCGCTATCGTG+AGG | + | chr7.3:6369965-6369984 | MS.gene06977:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6369836 | 6370978 | 6369836 | ID=MS.gene06977 |
| chr7.3 | mRNA | 6369836 | 6370978 | 6369836 | ID=MS.gene06977.t1;Parent=MS.gene06977 |
| chr7.3 | exon | 6369836 | 6369953 | 6369836 | ID=MS.gene06977.t1.exon1;Parent=MS.gene06977.t1 |
| chr7.3 | CDS | 6369836 | 6369953 | 6369836 | ID=cds.MS.gene06977.t1;Parent=MS.gene06977.t1 |
| chr7.3 | exon | 6370325 | 6370724 | 6370325 | ID=MS.gene06977.t1.exon2;Parent=MS.gene06977.t1 |
| chr7.3 | CDS | 6370325 | 6370724 | 6370325 | ID=cds.MS.gene06977.t1;Parent=MS.gene06977.t1 |
| chr7.3 | exon | 6370822 | 6370978 | 6370822 | ID=MS.gene06977.t1.exon3;Parent=MS.gene06977.t1 |
| chr7.3 | CDS | 6370822 | 6370978 | 6370822 | ID=cds.MS.gene06977.t1;Parent=MS.gene06977.t1 |
| Gene Sequence |
| Protein sequence |
