Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070069.t1 | XP_003616557.1 | 94.2 | 278 | 13 | 3 | 1 | 276 | 21 | 297 | 1.40E-138 | 502.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070069.t1 | O04195 | 37.1 | 267 | 146 | 5 | 1 | 264 | 23 | 270 | 1.7e-42 | 174.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070069.t1 | G7KBV9 | 94.2 | 278 | 13 | 3 | 1 | 276 | 21 | 297 | 1.0e-138 | 502.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055701 | MS.gene070069 | 0.802418 | 5.66E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070069.t1 | MTR_5g081740 | 94.245 | 278 | 13 | 3 | 1 | 276 | 21 | 297 | 0.0 | 508 |
| MS.gene070069.t1 | MTR_5g081740 | 94.762 | 210 | 10 | 1 | 1 | 209 | 21 | 230 | 1.27e-136 | 385 |
| MS.gene070069.t1 | MTR_5g081740 | 94.762 | 210 | 10 | 1 | 1 | 209 | 21 | 230 | 2.69e-136 | 384 |
| MS.gene070069.t1 | MTR_3g070090 | 61.818 | 275 | 96 | 4 | 5 | 273 | 28 | 299 | 2.27e-118 | 342 |
| MS.gene070069.t1 | MTR_5g081740 | 95.270 | 148 | 6 | 1 | 1 | 147 | 21 | 168 | 1.71e-91 | 268 |
| MS.gene070069.t1 | MTR_7g092870 | 92.969 | 128 | 9 | 0 | 1 | 128 | 21 | 148 | 4.44e-76 | 228 |
| MS.gene070069.t1 | MTR_7g092875 | 91.837 | 98 | 7 | 1 | 164 | 261 | 1 | 97 | 9.81e-60 | 185 |
| MS.gene070069.t1 | MTR_8g046260 | 28.302 | 212 | 137 | 6 | 66 | 266 | 47 | 254 | 1.71e-17 | 80.1 |
| MS.gene070069.t1 | MTR_6g090070 | 26.761 | 213 | 143 | 5 | 64 | 266 | 38 | 247 | 2.14e-14 | 71.6 |
| MS.gene070069.t1 | MTR_1g073610 | 24.706 | 255 | 172 | 8 | 21 | 266 | 5 | 248 | 3.13e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070069.t1 | AT2G39920 | 37.453 | 267 | 145 | 5 | 1 | 264 | 23 | 270 | 5.15e-57 | 184 |
| MS.gene070069.t1 | AT2G39920 | 37.453 | 267 | 145 | 5 | 1 | 264 | 23 | 270 | 5.15e-57 | 184 |
| MS.gene070069.t1 | AT2G39920 | 37.453 | 267 | 145 | 5 | 1 | 264 | 23 | 270 | 5.15e-57 | 184 |
| MS.gene070069.t1 | AT2G39920 | 37.079 | 267 | 145 | 5 | 1 | 264 | 23 | 269 | 3.97e-56 | 182 |
| MS.gene070069.t1 | AT4G29270 | 32.243 | 214 | 130 | 7 | 64 | 266 | 42 | 251 | 2.30e-22 | 93.6 |
| MS.gene070069.t1 | AT4G29260 | 29.778 | 225 | 129 | 8 | 60 | 266 | 37 | 250 | 2.58e-15 | 74.3 |
| MS.gene070069.t1 | AT4G25150 | 25.191 | 262 | 166 | 8 | 28 | 266 | 1 | 255 | 1.73e-14 | 72.0 |
| MS.gene070069.t1 | AT5G51260 | 24.599 | 187 | 126 | 5 | 65 | 239 | 44 | 227 | 1.37e-12 | 66.6 |
Find 39 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATGCTAAGCTCAATAATT+TGG | 0.229680 | 5.3:-66161622 | MS.gene070069:CDS |
| AAGGAATGTGATACAGAAAA+AGG | 0.244662 | 5.3:-66160127 | MS.gene070069:CDS |
| AAATTACCTTAGTAAGAAAT+TGG | 0.250800 | 5.3:+66161407 | None:intergenic |
| GTTGATTGATGGTGACATTT+TGG | 0.278987 | 5.3:+66160637 | None:intergenic |
| AATGAGGATTCCATTGAAAA+AGG | 0.315752 | 5.3:+66161433 | None:intergenic |
| ATTGCTGGATTTGCTGCTCT+TGG | 0.316792 | 5.3:-66161771 | MS.gene070069:CDS |
| CTTGTTGATGCTGGATTTAG+AGG | 0.329191 | 5.3:-66160615 | MS.gene070069:CDS |
| GTAATCCTCAATCACAGATT+TGG | 0.344974 | 5.3:+66161515 | None:intergenic |
| TCTGCAACAATCTTCATTGC+TGG | 0.362031 | 5.3:-66161786 | MS.gene070069:CDS |
| ATTCTTCCAATTTCTTACTA+AGG | 0.388067 | 5.3:-66161413 | MS.gene070069:intron |
| ATCAATCAACTTGTTGATGC+TGG | 0.420104 | 5.3:-66160624 | MS.gene070069:CDS |
| AGCATCAACAAGTTGATTGA+TGG | 0.423312 | 5.3:+66160626 | None:intergenic |
| GACAGGTCTTTACAAATGTT+AGG | 0.426632 | 5.3:+66161585 | None:intergenic |
| TAGACCAACCACTAGCTTGA+AGG | 0.432274 | 5.3:+66160691 | None:intergenic |
| ACTGTTACAGATGCAGATAC+AGG | 0.440440 | 5.3:-66160060 | MS.gene070069:CDS |
| AGTCACTCTGCTAGTTTCAA+TGG | 0.448449 | 5.3:-66161739 | MS.gene070069:CDS |
| TTGATGCTGGATTTAGAGGC+TGG | 0.460574 | 5.3:-66160611 | MS.gene070069:CDS |
| TCTTGTCAAAATAGCAATGC+TGG | 0.467391 | 5.3:-66161702 | MS.gene070069:CDS |
| TTCAACCAAATCTGTGATTG+AGG | 0.476332 | 5.3:-66161520 | MS.gene070069:CDS |
| TCAAACATGTCACATGGGTC+TGG | 0.485425 | 5.3:+66160018 | None:intergenic |
| GTGATTGAGGATTACTTCAA+TGG | 0.491347 | 5.3:-66161507 | MS.gene070069:CDS |
| AGTAAGAAATTGGAAGAATG+AGG | 0.498371 | 5.3:+66161417 | None:intergenic |
| GGTGTTAAACCATCAGAAGA+TGG | 0.502777 | 5.3:-66161486 | MS.gene070069:CDS |
| ATGCTGGAACTATTGAGCTT+AGG | 0.510219 | 5.3:-66161686 | MS.gene070069:CDS |
| CTTTGATATATTGTAAAGAC+AGG | 0.522975 | 5.3:+66161568 | None:intergenic |
| GAAGATGAAGATTCTACCAA+AGG | 0.561248 | 5.3:-66160171 | MS.gene070069:CDS |
| GGTCTTCATTGCTGATGATG+AGG | 0.581203 | 5.3:-66160590 | MS.gene070069:intron |
| TGGTCAATATGCAAGAGACT+TGG | 0.581585 | 5.3:-66161544 | MS.gene070069:CDS |
| TGAGGCTCAAACATGTCACA+TGG | 0.587978 | 5.3:+66160012 | None:intergenic |
| TACAAGAACCTTCAAGCTAG+TGG | 0.590030 | 5.3:-66160699 | MS.gene070069:CDS |
| ACATCAAAACCATCTTCTGA+TGG | 0.590480 | 5.3:+66161477 | None:intergenic |
| AAGTATCATAAGCAGTCATG+TGG | 0.611564 | 5.3:-66160091 | MS.gene070069:CDS |
| TGAGCCTCAGAGGAGAGCAT+AGG | 0.617171 | 5.3:-66159998 | None:intergenic |
| AGCACCTATGCTCTCCTCTG+AGG | 0.619362 | 5.3:+66159994 | None:intergenic |
| AGAACCTTCAAGCTAGTGGT+TGG | 0.622868 | 5.3:-66160695 | MS.gene070069:CDS |
| GTGACATGTTTGAGCCTCAG+AGG | 0.625870 | 5.3:-66160008 | MS.gene070069:CDS |
| TCTTTACAATATATCAAAGG+TGG | 0.628623 | 5.3:-66161564 | MS.gene070069:CDS |
| CTGTCTTTACAATATATCAA+AGG | 0.633916 | 5.3:-66161567 | MS.gene070069:CDS |
| GAGGCTCAAACATGTCACAT+GGG | 0.662491 | 5.3:+66160013 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTATCATTTAAGATT+TGG | - | chr5.3:66160443-66160462 | MS.gene070069:intron | 10.0% |
| !! | TCTTTACTAATATTGTAAAA+CGG | + | chr5.3:66160663-66160682 | None:intergenic | 15.0% |
| !!! | ATTTCTATTAGTTTAGTTTT+TGG | - | chr5.3:66160471-66160490 | MS.gene070069:intron | 15.0% |
| !!! | TTTCTATTAGTTTAGTTTTT+GGG | - | chr5.3:66160472-66160491 | MS.gene070069:intron | 15.0% |
| !! | AAATTACCTTAGTAAGAAAT+TGG | + | chr5.3:66160429-66160448 | None:intergenic | 20.0% |
| !! | AGAAAAATATTCATTTCCTT+TGG | + | chr5.3:66161681-66161700 | None:intergenic | 20.0% |
| !! | CTTTACTAATATTGTAAAAC+GGG | + | chr5.3:66160662-66160681 | None:intergenic | 20.0% |
| !! | TTTACTAATATTGTAAAACG+GGG | + | chr5.3:66160661-66160680 | None:intergenic | 20.0% |
| !!! | ATGAATATTTTTCTAGACAA+AGG | - | chr5.3:66161687-66161706 | MS.gene070069:CDS | 20.0% |
| !!! | CTTAACATTATCTAGAATTA+AGG | + | chr5.3:66160832-66160851 | None:intergenic | 20.0% |
| !!! | TGTTGTTTTATAGATATCTA+AGG | - | chr5.3:66161317-66161336 | MS.gene070069:intron | 20.0% |
| ! | AGAATTAAGGTTGTACATAA+TGG | + | chr5.3:66160819-66160838 | None:intergenic | 25.0% |
| ! | AGACAAATCGAACATTAATA+TGG | + | chr5.3:66160603-66160622 | None:intergenic | 25.0% |
| ! | ATAGATAAATGCTAAGATCT+AGG | - | chr5.3:66160853-66160872 | MS.gene070069:intron | 25.0% |
| ! | ATTAGTAAACTAATGCAAGT+TGG | + | chr5.3:66160915-66160934 | None:intergenic | 25.0% |
| ! | ATTCTTCCAATTTCTTACTA+AGG | - | chr5.3:66160420-66160439 | MS.gene070069:intron | 25.0% |
| ! | CTGTCTTTACAATATATCAA+AGG | - | chr5.3:66160266-66160285 | MS.gene070069:intron | 25.0% |
| ! | CTTTGATATATTGTAAAGAC+AGG | + | chr5.3:66160268-66160287 | None:intergenic | 25.0% |
| ! | GGATTTGGATTTCATTTAAT+CGG | - | chr5.3:66160493-66160512 | MS.gene070069:intron | 25.0% |
| ! | TATCTATAAAACAACATCTG+TGG | + | chr5.3:66161313-66161332 | None:intergenic | 25.0% |
| ! | TCTGCAAAAATAGAACATAT+TGG | + | chr5.3:66161639-66161658 | None:intergenic | 25.0% |
| ! | TCTTTACAATATATCAAAGG+TGG | - | chr5.3:66160269-66160288 | MS.gene070069:intron | 25.0% |
| ! | TTAGAGCTTCAACAAAATTA+CGG | - | chr5.3:66161492-66161511 | MS.gene070069:CDS | 25.0% |
| ! | TTGATTACAAAGTACATGTT+TGG | - | chr5.3:66161409-66161428 | MS.gene070069:intron | 25.0% |
| !! | AACCATTATCTAGGATATAT+GGG | + | chr5.3:66161281-66161300 | None:intergenic | 25.0% |
| !! | AGCTTTTATCAAAATCACAA+TGG | + | chr5.3:66161467-66161486 | None:intergenic | 25.0% |
| !! | TTTTTCTTCAACCATTATCT+AGG | + | chr5.3:66161290-66161309 | None:intergenic | 25.0% |
| !!! | TTAGTTTAGTTTTTGGGATT+TGG | - | chr5.3:66160478-66160497 | MS.gene070069:intron | 25.0% |
| AACATGTCACATATACTTTG+TGG | + | chr5.3:66161551-66161570 | None:intergenic | 30.0% | |
| AAGGAATGTGATACAGAAAA+AGG | - | chr5.3:66161706-66161725 | MS.gene070069:CDS | 30.0% | |
| AATGAGGATTCCATTGAAAA+AGG | + | chr5.3:66160403-66160422 | None:intergenic | 30.0% | |
| ACATGCTAAGCTCAATAATT+TGG | - | chr5.3:66160211-66160230 | MS.gene070069:intron | 30.0% | |
| AGGAATGTGATACAGAAAAA+GGG | - | chr5.3:66161707-66161726 | MS.gene070069:CDS | 30.0% | |
| AGTAAGAAATTGGAAGAATG+AGG | + | chr5.3:66160419-66160438 | None:intergenic | 30.0% | |
| TAAAACTATACATCATGAGC+CGG | + | chr5.3:66160539-66160558 | None:intergenic | 30.0% | |
| TGCAGATTAGTATACTAATC+TGG | + | chr5.3:66160996-66161015 | None:intergenic | 30.0% | |
| ! | AACATGACAAGGTTTTCTTT+TGG | - | chr5.3:66161031-66161050 | MS.gene070069:intron | 30.0% |
| ! | ACATATTGGTGCTTAAAATG+TGG | + | chr5.3:66161625-66161644 | None:intergenic | 30.0% |
| ! | CAACCATTATCTAGGATATA+TGG | + | chr5.3:66161282-66161301 | None:intergenic | 30.0% |
| !! | ATTTTGTTATCAAGAGAGTC+AGG | - | chr5.3:66161167-66161186 | MS.gene070069:intron | 30.0% |
| !!! | ACATAGATAGCCTTTTTCAA+TGG | - | chr5.3:66160390-66160409 | MS.gene070069:intron | 30.0% |
| AAGTATCATAAGCAGTCATG+TGG | - | chr5.3:66161742-66161761 | MS.gene070069:CDS | 35.0% | |
| ACATCAAAACCATCTTCTGA+TGG | + | chr5.3:66160359-66160378 | None:intergenic | 35.0% | |
| ATTCTTGACTGCGAGTAATT+CGG | - | chr5.3:66160633-66160652 | MS.gene070069:CDS | 35.0% | |
| CAATGAGTTTGGCAAAATCA+TGG | + | chr5.3:66161523-66161542 | None:intergenic | 35.0% | |
| CACCCATATATCCTAGATAA+TGG | - | chr5.3:66161276-66161295 | MS.gene070069:intron | 35.0% | |
| GAAGATGAAGATTCTACCAA+AGG | - | chr5.3:66161662-66161681 | MS.gene070069:CDS | 35.0% | |
| GACAGGTCTTTACAAATGTT+AGG | + | chr5.3:66160251-66160270 | None:intergenic | 35.0% | |
| GTAATCCTCAATCACAGATT+TGG | + | chr5.3:66160321-66160340 | None:intergenic | 35.0% | |
| GTGATTGAGGATTACTTCAA+TGG | - | chr5.3:66160326-66160345 | MS.gene070069:intron | 35.0% | |
| TCACATGCTTTGTGTATGTT+TGG | + | chr5.3:66161599-66161618 | None:intergenic | 35.0% | |
| TCTTGTCAAAATAGCAATGC+TGG | - | chr5.3:66160131-66160150 | MS.gene070069:CDS | 35.0% | |
| TGATACAGAAAAAGGGTTTC+AGG | - | chr5.3:66161714-66161733 | MS.gene070069:CDS | 35.0% | |
| TTCAACCAAATCTGTGATTG+AGG | - | chr5.3:66160313-66160332 | MS.gene070069:intron | 35.0% | |
| TTGTGGATGAACAATGAGTT+TGG | + | chr5.3:66161534-66161553 | None:intergenic | 35.0% | |
| TTGTTAGCAGTAACATGACA+AGG | - | chr5.3:66161020-66161039 | MS.gene070069:intron | 35.0% | |
| ! | CATTATCTAGGATATATGGG+TGG | + | chr5.3:66161278-66161297 | None:intergenic | 35.0% |
| !! | ACAAGGTTTTCTTTTGGTGT+TGG | - | chr5.3:66161037-66161056 | MS.gene070069:intron | 35.0% |
| !! | AGCATCAACAAGTTGATTGA+TGG | + | chr5.3:66161210-66161229 | None:intergenic | 35.0% |
| !! | AGGTTGTACATAATGGATAG+AGG | + | chr5.3:66160812-66160831 | None:intergenic | 35.0% |
| !! | ATCAATCAACTTGTTGATGC+TGG | - | chr5.3:66161209-66161228 | MS.gene070069:intron | 35.0% |
| !!! | AGTAGTTTTGTGCTTGAATC+AGG | - | chr5.3:66160005-66160024 | MS.gene070069:CDS | 35.0% |
| !!! | ATCAGAAGATGGTTTTGATG+TGG | - | chr5.3:66160358-66160377 | MS.gene070069:intron | 35.0% |
| !!! | GTTGATTGATGGTGACATTT+TGG | + | chr5.3:66161199-66161218 | None:intergenic | 35.0% |
| AAACTTAAAGTCGAGATGGC+CGG | - | chr5.3:66160517-66160536 | MS.gene070069:intron | 40.0% | |
| ACTGTTACAGATGCAGATAC+AGG | - | chr5.3:66161773-66161792 | MS.gene070069:CDS | 40.0% | |
| ATGCTGGAACTATTGAGCTT+AGG | - | chr5.3:66160147-66160166 | MS.gene070069:CDS | 40.0% | |
| CGGAAAACTTAAAGTCGAGA+TGG | - | chr5.3:66160513-66160532 | MS.gene070069:intron | 40.0% | |
| CTCGCAGTCAAGAATCTATA+CGG | + | chr5.3:66160628-66160647 | None:intergenic | 40.0% | |
| CTTGTTGATGCTGGATTTAG+AGG | - | chr5.3:66161218-66161237 | MS.gene070069:intron | 40.0% | |
| GGTGTTAAACCATCAGAAGA+TGG | - | chr5.3:66160347-66160366 | MS.gene070069:intron | 40.0% | |
| TACAAGAACCTTCAAGCTAG+TGG | - | chr5.3:66161134-66161153 | MS.gene070069:intron | 40.0% | |
| TCTGCAACAATCTTCATTGC+TGG | - | chr5.3:66160047-66160066 | MS.gene070069:CDS | 40.0% | |
| TGAGTTTGGCAAAATCATGG+CGG | + | chr5.3:66161520-66161539 | None:intergenic | 40.0% | |
| TGGTCAATATGCAAGAGACT+TGG | - | chr5.3:66160289-66160308 | MS.gene070069:intron | 40.0% | |
| ! | AGTCACTCTGCTAGTTTCAA+TGG | - | chr5.3:66160094-66160113 | MS.gene070069:CDS | 40.0% |
| AGAACCTTCAAGCTAGTGGT+TGG | - | chr5.3:66161138-66161157 | MS.gene070069:intron | 45.0% | |
| GAGGCTCAAACATGTCACAT+GGG | + | chr5.3:66161823-66161842 | None:intergenic | 45.0% | |
| TCAAACATGTCACATGGGTC+TGG | + | chr5.3:66161818-66161837 | None:intergenic | 45.0% | |
| TGAGGCTCAAACATGTCACA+TGG | + | chr5.3:66161824-66161843 | None:intergenic | 45.0% | |
| TTGATGCTGGATTTAGAGGC+TGG | - | chr5.3:66161222-66161241 | MS.gene070069:intron | 45.0% | |
| ! | ATGCTAAGATCTAGGACAGC+TGG | - | chr5.3:66160861-66160880 | MS.gene070069:intron | 45.0% |
| ! | GGTCTTCATTGCTGATGATG+AGG | - | chr5.3:66161243-66161262 | MS.gene070069:intron | 45.0% |
| ! | TAGACCAACCACTAGCTTGA+AGG | + | chr5.3:66161145-66161164 | None:intergenic | 45.0% |
| !! | ATTGCTGGATTTGCTGCTCT+TGG | - | chr5.3:66160062-66160081 | MS.gene070069:CDS | 45.0% |
| GTGACATGTTTGAGCCTCAG+AGG | - | chr5.3:66161825-66161844 | MS.gene070069:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 66159999 | 66161856 | 66159999 | ID=MS.gene070069 |
| chr5.3 | mRNA | 66159999 | 66161856 | 66159999 | ID=MS.gene070069.t1;Parent=MS.gene070069 |
| chr5.3 | exon | 66161414 | 66161856 | 66161414 | ID=MS.gene070069.t1.exon1;Parent=MS.gene070069.t1 |
| chr5.3 | CDS | 66161414 | 66161856 | 66161414 | ID=cds.MS.gene070069.t1;Parent=MS.gene070069.t1 |
| chr5.3 | exon | 66160591 | 66160779 | 66160591 | ID=MS.gene070069.t1.exon2;Parent=MS.gene070069.t1 |
| chr5.3 | CDS | 66160591 | 66160779 | 66160591 | ID=cds.MS.gene070069.t1;Parent=MS.gene070069.t1 |
| chr5.3 | exon | 66159999 | 66160197 | 66159999 | ID=MS.gene070069.t1.exon3;Parent=MS.gene070069.t1 |
| chr5.3 | CDS | 66159999 | 66160197 | 66159999 | ID=cds.MS.gene070069.t1;Parent=MS.gene070069.t1 |
| Gene Sequence |
| Protein sequence |
