Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070203.t1 | KEH33791.1 | 92.1 | 254 | 16 | 1 | 107 | 356 | 27 | 280 | 8.50E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070203.t1 | Q6JN46 | 35.0 | 103 | 60 | 1 | 259 | 354 | 914 | 1016 | 1.7e-10 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070203.t1 | A0A072UWJ3 | 92.1 | 254 | 16 | 1 | 107 | 356 | 27 | 280 | 6.1e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054108 | MS.gene070203 | 0.800156 | 1.64E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 71 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGATTTGCAGCAGGTTTC+TGG | -0.003578 | 3.2:+41605144 | MS.gene070203:CDS |
| ACAATTGCAGATCATGATTT+TGG | 0.106542 | 3.2:+41604410 | MS.gene070203:CDS |
| AGAGGAGCTCCGCATAATTT+TGG | 0.204687 | 3.2:-41605029 | None:intergenic |
| CTTTGTTTCTTATTAGAAAA+TGG | 0.268683 | 3.2:+41605180 | MS.gene070203:CDS |
| CTTGCTTTAAACAAACTTTC+AGG | 0.270380 | 3.2:+41604567 | MS.gene070203:CDS |
| GTTTGAATTTGTGATCCTAT+TGG | 0.278276 | 3.2:-41604978 | None:intergenic |
| TCAGCCTTATTTGTTTCATT+TGG | 0.293427 | 3.2:+41604542 | MS.gene070203:CDS |
| TTTAATGCATCAAGCTTCAT+TGG | 0.302889 | 3.2:+41605002 | MS.gene070203:CDS |
| TTTGAATTTGTGATCCTATT+GGG | 0.304698 | 3.2:-41604977 | None:intergenic |
| TTCATGCCTCCAATCACTTT+TGG | 0.306951 | 3.2:-41604834 | None:intergenic |
| CAAGATCTATGTTACTTTGA+TGG | 0.318777 | 3.2:+41605241 | MS.gene070203:CDS |
| TTATCTCACAATAATTTGAT+TGG | 0.328914 | 3.2:+41604804 | MS.gene070203:CDS |
| TTGGGATAGTCCCTTCAAAT+TGG | 0.334248 | 3.2:-41604506 | None:intergenic |
| ATCAAAGAATCAATGTATCT+TGG | 0.339893 | 3.2:+41605113 | MS.gene070203:CDS |
| AGGTCAATAGTTCTTCTTCT+TGG | 0.356656 | 3.2:-41604711 | None:intergenic |
| CTTTCAGCAAATAGCTTGTC+TGG | 0.358680 | 3.2:+41604732 | MS.gene070203:CDS |
| TGGATTTGCAGCAGGTTTCT+GGG | 0.365964 | 3.2:+41605145 | MS.gene070203:CDS |
| TGTCTATAACTTGACTGATA+TGG | 0.381266 | 3.2:+41604605 | MS.gene070203:CDS |
| CGGGATCAATTCCACATAGT+TGG | 0.382996 | 3.2:+41604304 | MS.gene070203:CDS |
| AGGTCCAAATGAAACAAATA+AGG | 0.385591 | 3.2:-41604546 | None:intergenic |
| CTTTGATGGTAAAGTTGAAC+AGG | 0.394974 | 3.2:+41605255 | MS.gene070203:CDS |
| TTGGATAACAATAATTTCAT+AGG | 0.401019 | 3.2:+41604222 | MS.gene070203:CDS |
| TAAGCCATTGGTAATATGTT+CGG | 0.403644 | 3.2:-41604252 | None:intergenic |
| TTTGATGGAACAATCCCAAT+AGG | 0.408333 | 3.2:+41604963 | MS.gene070203:CDS |
| AATGGAGGCATGCATGCTTT+CGG | 0.421454 | 3.2:+41605198 | MS.gene070203:CDS |
| CTTGTCTGGAGAAGTGCCTT+TGG | 0.428859 | 3.2:+41604746 | MS.gene070203:CDS |
| TCAAAGAATCAATGTATCTT+GGG | 0.429179 | 3.2:+41605114 | MS.gene070203:CDS |
| ACAATTGAGTTGTTTACAAA+AGG | 0.429802 | 3.2:+41604663 | MS.gene070203:CDS |
| TTGTGATAAGTTCATTGGTA+TGG | 0.438242 | 3.2:-41604454 | None:intergenic |
| TTGTCTTACAACTCATTCTC+GGG | 0.439306 | 3.2:+41604285 | MS.gene070203:CDS |
| ATGGGAGTTGGATTTGCAGC+AGG | 0.443030 | 3.2:+41605137 | MS.gene070203:CDS |
| TTTGTCTTACAACTCATTCT+CGG | 0.450815 | 3.2:+41604284 | MS.gene070203:CDS |
| ATGTATCTTGGGATGGGAGT+TGG | 0.465620 | 3.2:+41605125 | MS.gene070203:CDS |
| GCTGATTGAATAATTGCGTT+GGG | 0.470289 | 3.2:-41604524 | None:intergenic |
| TCATCTTCATTCTGTGTAGA+TGG | 0.471699 | 3.2:-41605083 | None:intergenic |
| AAGCCATTGGTAATATGTTC+GGG | 0.473321 | 3.2:-41604251 | None:intergenic |
| TTCAAGTTCTTCCAACTATG+TGG | 0.478919 | 3.2:-41604315 | None:intergenic |
| GGATTGGTACAATTATTAAG+AGG | 0.479712 | 3.2:-41605047 | None:intergenic |
| GGCTGATTGAATAATTGCGT+TGG | 0.489338 | 3.2:-41604525 | None:intergenic |
| TTGAGAGCTAACCAATTTGA+AGG | 0.489346 | 3.2:+41604495 | MS.gene070203:CDS |
| CTGAAAGTTTGTTTAAAGCA+AGG | 0.499891 | 3.2:-41604566 | None:intergenic |
| AACTCAATTGTGGTACCTGC+TGG | 0.505072 | 3.2:-41604651 | None:intergenic |
| TCATTCTGTGTAGATGGCGT+TGG | 0.510792 | 3.2:-41605077 | None:intergenic |
| TTGTGGAGTAATAAGCTATC+CGG | 0.513583 | 3.2:+41604357 | MS.gene070203:CDS |
| TGGAGAAATTCCACCGAGCA+TGG | 0.522897 | 3.2:+41604896 | MS.gene070203:CDS |
| AGAATCAATGTATCTTGGGA+TGG | 0.523720 | 3.2:+41605118 | MS.gene070203:CDS |
| GGATTTGCAGCAGGTTTCTG+GGG | 0.524214 | 3.2:+41605146 | MS.gene070203:CDS |
| ATTTATATTTCATTAACTTG+TGG | 0.526189 | 3.2:+41604340 | MS.gene070203:CDS |
| AAATCAACTGCATAAGCCAT+TGG | 0.549986 | 3.2:-41604264 | None:intergenic |
| CTTTCGGTTTGTTGATAGAG+TGG | 0.550163 | 3.2:+41605214 | MS.gene070203:CDS |
| GATAACAATAATTTCATAGG+AGG | 0.551425 | 3.2:+41604225 | MS.gene070203:CDS |
| GAATCAATGTATCTTGGGAT+GGG | 0.556531 | 3.2:+41605119 | MS.gene070203:CDS |
| GATTTGCAGCAGGTTTCTGG+GGG | 0.570767 | 3.2:+41605147 | MS.gene070203:CDS |
| AAAATACCAAAAGTGATTGG+AGG | 0.571063 | 3.2:+41604828 | MS.gene070203:CDS |
| GAAGAGGAGGAATTACCAGC+AGG | 0.575112 | 3.2:+41604636 | MS.gene070203:CDS |
| CAGACAAGCTATTTGCTGAA+AGG | 0.576647 | 3.2:-41604731 | None:intergenic |
| TTTCGGTTTGTTGATAGAGT+GGG | 0.585715 | 3.2:+41605215 | MS.gene070203:CDS |
| TATGGTTACATTTGAAGAGG+AGG | 0.587819 | 3.2:+41604623 | MS.gene070203:CDS |
| TGATATGGTTACATTTGAAG+AGG | 0.594849 | 3.2:+41604620 | MS.gene070203:CDS |
| TGAGAGCTAACCAATTTGAA+GGG | 0.599073 | 3.2:+41604496 | MS.gene070203:CDS |
| GAGGTGCTGTATAACTTCAC+CGG | 0.599733 | 3.2:-41604376 | None:intergenic |
| GGCTCTCTTAAACTTTCTAG+AGG | 0.607477 | 3.2:+41604917 | MS.gene070203:CDS |
| ATTGGAAATCCAAAATTATG+CGG | 0.613640 | 3.2:+41605020 | MS.gene070203:CDS |
| TGTTTCTTATTAGAAAATGG+AGG | 0.620884 | 3.2:+41605183 | MS.gene070203:CDS |
| AAGTTTAAGAGAGCCATGCT+CGG | 0.628881 | 3.2:-41604909 | None:intergenic |
| GTCAAGTTATAGACACACTC+TGG | 0.643071 | 3.2:-41604597 | None:intergenic |
| TTATACAGCACCTCTCTGAG+TGG | 0.661294 | 3.2:+41604385 | MS.gene070203:CDS |
| TTTAAGAGAGCCATGCTCGG+TGG | 0.664745 | 3.2:-41604906 | None:intergenic |
| ACTCCCGAACATATTACCAA+TGG | 0.666984 | 3.2:+41604248 | MS.gene070203:CDS |
| ACAAGTCGAAACAATTCCAA+AGG | 0.668877 | 3.2:-41604762 | None:intergenic |
| GCAATTGTTTCCACTCAGAG+AGG | 0.685150 | 3.2:-41604395 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATATTTCATTAACTTG+TGG | + | chr3.2:41604340-41604359 | MS.gene070203:CDS | 15.0% |
| !! | TTATCTCACAATAATTTGAT+TGG | + | chr3.2:41604804-41604823 | MS.gene070203:CDS | 20.0% |
| !! | TTGGATAACAATAATTTCAT+AGG | + | chr3.2:41604222-41604241 | MS.gene070203:CDS | 20.0% |
| !!! | AATTTTTGTGATAAGTTCAT+TGG | - | chr3.2:41604462-41604481 | None:intergenic | 20.0% |
| !!! | CTCTCAAATAATAAGTTTTT+TGG | + | chr3.2:41604876-41604895 | MS.gene070203:CDS | 20.0% |
| !!! | CTTTGTTTCTTATTAGAAAA+TGG | + | chr3.2:41605180-41605199 | MS.gene070203:CDS | 20.0% |
| !!! | TTATCTTGCAATAATTTTGA+TGG | + | chr3.2:41604948-41604967 | MS.gene070203:CDS | 20.0% |
| ! | ATCAAAGAATCAATGTATCT+TGG | + | chr3.2:41605113-41605132 | MS.gene070203:CDS | 25.0% |
| ! | ATTGGAAATCCAAAATTATG+CGG | + | chr3.2:41605020-41605039 | MS.gene070203:CDS | 25.0% |
| ! | GATAACAATAATTTCATAGG+AGG | + | chr3.2:41604225-41604244 | MS.gene070203:CDS | 25.0% |
| ! | TCAAAGAATCAATGTATCTT+GGG | + | chr3.2:41605114-41605133 | MS.gene070203:CDS | 25.0% |
| ! | TGTTTCTTATTAGAAAATGG+AGG | + | chr3.2:41605183-41605202 | MS.gene070203:CDS | 25.0% |
| ! | TTTGAATTTGTGATCCTATT+GGG | - | chr3.2:41604980-41604999 | None:intergenic | 25.0% |
| !! | ACAATTGAGTTGTTTACAAA+AGG | + | chr3.2:41604663-41604682 | MS.gene070203:CDS | 25.0% |
| !! | TTGGGAGAAAATAAATTTTC+AGG | + | chr3.2:41604429-41604448 | MS.gene070203:CDS | 25.0% |
| !! | TTTTGTAAACAACTCAATTG+TGG | - | chr3.2:41604664-41604683 | None:intergenic | 25.0% |
| AAAATACCAAAAGTGATTGG+AGG | + | chr3.2:41604828-41604847 | MS.gene070203:CDS | 30.0% | |
| AGGTCCAAATGAAACAAATA+AGG | - | chr3.2:41604549-41604568 | None:intergenic | 30.0% | |
| GGAAAAATACCAAAAGTGAT+TGG | + | chr3.2:41604825-41604844 | MS.gene070203:CDS | 30.0% | |
| GTTTGAATTTGTGATCCTAT+TGG | - | chr3.2:41604981-41605000 | None:intergenic | 30.0% | |
| TGATATGGTTACATTTGAAG+AGG | + | chr3.2:41604620-41604639 | MS.gene070203:CDS | 30.0% | |
| TGTCTATAACTTGACTGATA+TGG | + | chr3.2:41604605-41604624 | MS.gene070203:CDS | 30.0% | |
| TTGTGATAAGTTCATTGGTA+TGG | - | chr3.2:41604457-41604476 | None:intergenic | 30.0% | |
| TTTAATGCATCAAGCTTCAT+TGG | + | chr3.2:41605002-41605021 | MS.gene070203:CDS | 30.0% | |
| TTTGTCTTACAACTCATTCT+CGG | + | chr3.2:41604284-41604303 | MS.gene070203:CDS | 30.0% | |
| ! | ACAATTGCAGATCATGATTT+TGG | + | chr3.2:41604410-41604429 | MS.gene070203:CDS | 30.0% |
| ! | CAATTGCAGATCATGATTTT+GGG | + | chr3.2:41604411-41604430 | MS.gene070203:CDS | 30.0% |
| ! | CTGAAAGTTTGTTTAAAGCA+AGG | - | chr3.2:41604569-41604588 | None:intergenic | 30.0% |
| ! | CTTGCTTTAAACAAACTTTC+AGG | + | chr3.2:41604567-41604586 | MS.gene070203:CDS | 30.0% |
| ! | GGATTGGTACAATTATTAAG+AGG | - | chr3.2:41605050-41605069 | None:intergenic | 30.0% |
| ! | TAAGCCATTGGTAATATGTT+CGG | - | chr3.2:41604255-41604274 | None:intergenic | 30.0% |
| !! | CAAGATCTATGTTACTTTGA+TGG | + | chr3.2:41605241-41605260 | MS.gene070203:CDS | 30.0% |
| !! | TCAGCCTTATTTGTTTCATT+TGG | + | chr3.2:41604542-41604561 | MS.gene070203:CDS | 30.0% |
| AAATCAACTGCATAAGCCAT+TGG | - | chr3.2:41604267-41604286 | None:intergenic | 35.0% | |
| ACAAGTCGAAACAATTCCAA+AGG | - | chr3.2:41604765-41604784 | None:intergenic | 35.0% | |
| AGAATCAATGTATCTTGGGA+TGG | + | chr3.2:41605118-41605137 | MS.gene070203:CDS | 35.0% | |
| AGGTCAATAGTTCTTCTTCT+TGG | - | chr3.2:41604714-41604733 | None:intergenic | 35.0% | |
| GAATCAATGTATCTTGGGAT+GGG | + | chr3.2:41605119-41605138 | MS.gene070203:CDS | 35.0% | |
| GCTGATTGAATAATTGCGTT+GGG | - | chr3.2:41604527-41604546 | None:intergenic | 35.0% | |
| TATGGTTACATTTGAAGAGG+AGG | + | chr3.2:41604623-41604642 | MS.gene070203:CDS | 35.0% | |
| TCATCTTCATTCTGTGTAGA+TGG | - | chr3.2:41605086-41605105 | None:intergenic | 35.0% | |
| TGAGAGCTAACCAATTTGAA+GGG | + | chr3.2:41604496-41604515 | MS.gene070203:CDS | 35.0% | |
| TTGAGAGCTAACCAATTTGA+AGG | + | chr3.2:41604495-41604514 | MS.gene070203:CDS | 35.0% | |
| TTGTCTTACAACTCATTCTC+GGG | + | chr3.2:41604285-41604304 | MS.gene070203:CDS | 35.0% | |
| TTGTGGAGTAATAAGCTATC+CGG | + | chr3.2:41604357-41604376 | MS.gene070203:CDS | 35.0% | |
| TTTGATGGAACAATCCCAAT+AGG | + | chr3.2:41604963-41604982 | MS.gene070203:CDS | 35.0% | |
| ! | AAGCCATTGGTAATATGTTC+GGG | - | chr3.2:41604254-41604273 | None:intergenic | 35.0% |
| ! | CTTTGATGGTAAAGTTGAAC+AGG | + | chr3.2:41605255-41605274 | MS.gene070203:CDS | 35.0% |
| ! | GATTGGAGGCATGAAAAATA+TGG | + | chr3.2:41604842-41604861 | MS.gene070203:CDS | 35.0% |
| ! | TTCAAGTTCTTCCAACTATG+TGG | - | chr3.2:41604318-41604337 | None:intergenic | 35.0% |
| !! | TTTCGGTTTGTTGATAGAGT+GGG | + | chr3.2:41605215-41605234 | MS.gene070203:CDS | 35.0% |
| AAGTTTAAGAGAGCCATGCT+CGG | - | chr3.2:41604912-41604931 | None:intergenic | 40.0% | |
| ACTCCCGAACATATTACCAA+TGG | + | chr3.2:41604248-41604267 | MS.gene070203:CDS | 40.0% | |
| GGCTCTCTTAAACTTTCTAG+AGG | + | chr3.2:41604917-41604936 | MS.gene070203:CDS | 40.0% | |
| GGCTGATTGAATAATTGCGT+TGG | - | chr3.2:41604528-41604547 | None:intergenic | 40.0% | |
| GTCAAGTTATAGACACACTC+TGG | - | chr3.2:41604600-41604619 | None:intergenic | 40.0% | |
| TTGGGATAGTCCCTTCAAAT+TGG | - | chr3.2:41604509-41604528 | None:intergenic | 40.0% | |
| ! | CAGACAAGCTATTTGCTGAA+AGG | - | chr3.2:41604734-41604753 | None:intergenic | 40.0% |
| ! | CTTTCAGCAAATAGCTTGTC+TGG | + | chr3.2:41604732-41604751 | MS.gene070203:CDS | 40.0% |
| ! | TTCATGCCTCCAATCACTTT+TGG | - | chr3.2:41604837-41604856 | None:intergenic | 40.0% |
| !! | CTTTCGGTTTGTTGATAGAG+TGG | + | chr3.2:41605214-41605233 | MS.gene070203:CDS | 40.0% |
| AACTCAATTGTGGTACCTGC+TGG | - | chr3.2:41604654-41604673 | None:intergenic | 45.0% | |
| ATGTATCTTGGGATGGGAGT+TGG | + | chr3.2:41605125-41605144 | MS.gene070203:CDS | 45.0% | |
| CGGGATCAATTCCACATAGT+TGG | + | chr3.2:41604304-41604323 | MS.gene070203:CDS | 45.0% | |
| GCAATTGTTTCCACTCAGAG+AGG | - | chr3.2:41604398-41604417 | None:intergenic | 45.0% | |
| TCATTCTGTGTAGATGGCGT+TGG | - | chr3.2:41605080-41605099 | None:intergenic | 45.0% | |
| TGGATTTGCAGCAGGTTTCT+GGG | + | chr3.2:41605145-41605164 | MS.gene070203:CDS | 45.0% | |
| TTATACAGCACCTCTCTGAG+TGG | + | chr3.2:41604385-41604404 | MS.gene070203:CDS | 45.0% | |
| TTGGATTTGCAGCAGGTTTC+TGG | + | chr3.2:41605144-41605163 | MS.gene070203:CDS | 45.0% | |
| ! | AATGGAGGCATGCATGCTTT+CGG | + | chr3.2:41605198-41605217 | MS.gene070203:CDS | 45.0% |
| ! | AGAGGAGCTCCGCATAATTT+TGG | - | chr3.2:41605032-41605051 | None:intergenic | 45.0% |
| !! | GAGGTGCTGTATAACTTCAC+CGG | - | chr3.2:41604379-41604398 | None:intergenic | 45.0% |
| !! | GTAGATGGCGTTGGAGTTTT+AGG | - | chr3.2:41605071-41605090 | None:intergenic | 45.0% |
| !!! | TGGCGTTGGAGTTTTAGGAT+TGG | - | chr3.2:41605066-41605085 | None:intergenic | 45.0% |
| ATGGGAGTTGGATTTGCAGC+AGG | + | chr3.2:41605137-41605156 | MS.gene070203:CDS | 50.0% | |
| GAAGAGGAGGAATTACCAGC+AGG | + | chr3.2:41604636-41604655 | MS.gene070203:CDS | 50.0% | |
| GGATTTGCAGCAGGTTTCTG+GGG | + | chr3.2:41605146-41605165 | MS.gene070203:CDS | 50.0% | |
| TGGAGAAATTCCACCGAGCA+TGG | + | chr3.2:41604896-41604915 | MS.gene070203:CDS | 50.0% | |
| TTTAAGAGAGCCATGCTCGG+TGG | - | chr3.2:41604909-41604928 | None:intergenic | 50.0% | |
| ! | GATTTGCAGCAGGTTTCTGG+GGG | + | chr3.2:41605147-41605166 | MS.gene070203:CDS | 50.0% |
| !! | CTTGTCTGGAGAAGTGCCTT+TGG | + | chr3.2:41604746-41604765 | MS.gene070203:CDS | 50.0% |
| ! | GCAGGTTTCTGGGGGATTTG+TGG | + | chr3.2:41605155-41605174 | MS.gene070203:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 41604220 | 41605292 | 41604220 | ID=MS.gene070203 |
| chr3.2 | mRNA | 41604220 | 41605292 | 41604220 | ID=MS.gene070203.t1;Parent=MS.gene070203 |
| chr3.2 | exon | 41604220 | 41605292 | 41604220 | ID=MS.gene070203.t1.exon1;Parent=MS.gene070203.t1 |
| chr3.2 | CDS | 41604220 | 41605292 | 41604220 | ID=cds.MS.gene070203.t1;Parent=MS.gene070203.t1 |
| Gene Sequence |
| Protein sequence |
