Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070209.t1 | RHN78012.1 | 89.8 | 215 | 22 | 0 | 1 | 215 | 267 | 481 | 4.30E-98 | 367.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070209.t1 | A0A072VGJ9 | 89.8 | 215 | 22 | 0 | 1 | 215 | 267 | 481 | 3.1e-98 | 367.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene070209 | 0.806893 | 6.59E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene070209 | 0.800313 | 1.53E-48 | -1.69E-46 |
| MS.gene050228 | MS.gene070209 | 0.803158 | 3.98E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene070209 | 0.814179 | 1.76E-51 | -1.69E-46 |
| MS.gene058833 | MS.gene070209 | 0.802647 | 5.08E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene070209 | 0.814758 | 1.31E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070209.t1 | MTR_1g031750 | 89.767 | 215 | 22 | 0 | 1 | 215 | 267 | 481 | 2.10e-135 | 390 |
| MS.gene070209.t1 | MTR_1g031750 | 89.767 | 215 | 22 | 0 | 1 | 215 | 267 | 481 | 1.27e-134 | 392 |
| MS.gene070209.t1 | MTR_1g031750 | 89.767 | 215 | 22 | 0 | 1 | 215 | 267 | 481 | 1.17e-132 | 392 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070209.t1 | AT1G23180 | 57.078 | 219 | 88 | 4 | 1 | 214 | 278 | 495 | 2.04e-68 | 221 |
| MS.gene070209.t1 | AT1G23180 | 57.078 | 219 | 88 | 4 | 1 | 214 | 278 | 495 | 2.76e-67 | 221 |
Find 36 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAACAAACCTTCTAAATT+TGG | 0.153821 | 1.3:-61400044 | MS.gene070209:CDS |
| GCAAAAGTCAATGCAATTAT+TGG | 0.230500 | 1.3:-61399966 | MS.gene070209:CDS |
| GGCGCATCAGAAGTACTTAT+TGG | 0.256084 | 1.3:-61400023 | MS.gene070209:CDS |
| ACGTGGGTTGTATGACTTAT+AGG | 0.260422 | 1.3:+61400115 | None:intergenic |
| TTAGTTCCTGTGCCATTAAT+AGG | 0.303101 | 1.3:-61400146 | MS.gene070209:CDS |
| TAGGCTGCAGCACCTATTAA+TGG | 0.337954 | 1.3:+61400134 | None:intergenic |
| GCTGGCAGCGACGCAAGCTT+TGG | 0.349598 | 1.3:-61399655 | MS.gene070209:CDS |
| TCTGATGCGCCAAATTTAGA+AGG | 0.361169 | 1.3:+61400035 | None:intergenic |
| AAGCTTGCGTCGCTGCCAGC+TGG | 0.396449 | 1.3:+61399658 | None:intergenic |
| TCTAATGACTTTAGATGCTT+CGG | 0.398255 | 1.3:+61400241 | None:intergenic |
| TTACAGAATTCTTGTTATTG+AGG | 0.445107 | 1.3:-61400174 | MS.gene070209:CDS |
| TTAGATGCTTCGGAGTCATA+TGG | 0.450507 | 1.3:+61400251 | None:intergenic |
| ACATATGCGTATTGCATTCA+AGG | 0.476617 | 1.3:-61399736 | MS.gene070209:CDS |
| TCAAGGAGGCTGGTGCAGTT+AGG | 0.484639 | 1.3:-61399719 | MS.gene070209:CDS |
| TCGTGTTGGTGCTATTGAAA+TGG | 0.486108 | 1.3:-61399919 | MS.gene070209:CDS |
| CAATGATAATGCAGTCCAGC+TGG | 0.486189 | 1.3:-61399673 | MS.gene070209:CDS |
| CGCGTCTGACACTCTTGCAT+TGG | 0.486741 | 1.3:-61399857 | MS.gene070209:CDS |
| AGAATTCTTGTTATTGAGGA+AGG | 0.486981 | 1.3:-61400170 | MS.gene070209:CDS |
| CGTATTGCATTCAAGGAGGC+TGG | 0.506096 | 1.3:-61399729 | MS.gene070209:CDS |
| AGCATCTAAAGTCATTAGAA+AGG | 0.509035 | 1.3:-61400237 | MS.gene070209:CDS |
| CAACAATTCCTTGTTCGTGT+TGG | 0.517289 | 1.3:-61399933 | MS.gene070209:CDS |
| TCTGTGCCATCTGGAAATGC+AGG | 0.528944 | 1.3:+61400081 | None:intergenic |
| ACACTCTTGCATTGGATCGA+CGG | 0.536563 | 1.3:-61399849 | MS.gene070209:CDS |
| CTTTGGAAAAGCTGTCTGCT+AGG | 0.538845 | 1.3:-61399638 | None:intergenic |
| GTGCAGTTAGGCATTTAGTA+CGG | 0.544923 | 1.3:-61399707 | MS.gene070209:CDS |
| TGCAGGCGCCTCGTATAACG+TGG | 0.590481 | 1.3:+61400098 | None:intergenic |
| CAATAGCACCAACACGAACA+AGG | 0.593972 | 1.3:+61399925 | None:intergenic |
| GCAGCACCTATTAATGGCAC+AGG | 0.594699 | 1.3:+61400140 | None:intergenic |
| TGCATTGCTAGAACTTGTGA+AGG | 0.603172 | 1.3:-61400204 | MS.gene070209:CDS |
| AAGTCATTAGAAAGGAAGCA+AGG | 0.621933 | 1.3:-61400229 | MS.gene070209:CDS |
| GAGGCGCCTGCATTTCCAGA+TGG | 0.635163 | 1.3:-61400087 | MS.gene070209:CDS |
| AGATGGCACAGAAAATCGAA+AGG | 0.659447 | 1.3:-61400070 | MS.gene070209:CDS |
| CAATGCAAGAGTGTCAGACG+CGG | 0.664864 | 1.3:+61399858 | None:intergenic |
| CATACAACCCACGTTATACG+AGG | 0.680894 | 1.3:-61400106 | MS.gene070209:CDS |
| GCAGGCGCCTCGTATAACGT+GGG | 0.716730 | 1.3:+61400099 | None:intergenic |
| TATGCGTATTGCATTCAAGG+AGG | 0.753564 | 1.3:-61399733 | MS.gene070209:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAACAAACCTTCTAAATT+TGG | - | chr1.3:61399858-61399877 | MS.gene070209:CDS | 20.0% |
| !!! | TTACAGAATTCTTGTTATTG+AGG | - | chr1.3:61399728-61399747 | MS.gene070209:CDS | 25.0% |
| GCAAAAGTCAATGCAATTAT+TGG | - | chr1.3:61399936-61399955 | MS.gene070209:CDS | 30.0% | |
| TCTAATGACTTTAGATGCTT+CGG | + | chr1.3:61399664-61399683 | None:intergenic | 30.0% | |
| ! | AGCATCTAAAGTCATTAGAA+AGG | - | chr1.3:61399665-61399684 | MS.gene070209:CDS | 30.0% |
| !! | AGAATTCTTGTTATTGAGGA+AGG | - | chr1.3:61399732-61399751 | MS.gene070209:CDS | 30.0% |
| AAGTCATTAGAAAGGAAGCA+AGG | - | chr1.3:61399673-61399692 | MS.gene070209:CDS | 35.0% | |
| ACATATGCGTATTGCATTCA+AGG | - | chr1.3:61400166-61400185 | MS.gene070209:CDS | 35.0% | |
| ! | TTAGTTCCTGTGCCATTAAT+AGG | - | chr1.3:61399756-61399775 | MS.gene070209:CDS | 35.0% |
| AGATGGCACAGAAAATCGAA+AGG | - | chr1.3:61399832-61399851 | MS.gene070209:CDS | 40.0% | |
| CAACAATTCCTTGTTCGTGT+TGG | - | chr1.3:61399969-61399988 | MS.gene070209:CDS | 40.0% | |
| GTGCAGTTAGGCATTTAGTA+CGG | - | chr1.3:61400195-61400214 | MS.gene070209:CDS | 40.0% | |
| TATGCGTATTGCATTCAAGG+AGG | - | chr1.3:61400169-61400188 | MS.gene070209:CDS | 40.0% | |
| TCTGATGCGCCAAATTTAGA+AGG | + | chr1.3:61399870-61399889 | None:intergenic | 40.0% | |
| TGCATTGCTAGAACTTGTGA+AGG | - | chr1.3:61399698-61399717 | MS.gene070209:CDS | 40.0% | |
| TTAGATGCTTCGGAGTCATA+TGG | + | chr1.3:61399654-61399673 | None:intergenic | 40.0% | |
| ! | ACGTGGGTTGTATGACTTAT+AGG | + | chr1.3:61399790-61399809 | None:intergenic | 40.0% |
| ! | TTTCGATTTTCTGTGCCATC+TGG | + | chr1.3:61399833-61399852 | None:intergenic | 40.0% |
| !! | TCGTGTTGGTGCTATTGAAA+TGG | - | chr1.3:61399983-61400002 | MS.gene070209:CDS | 40.0% |
| ACACTCTTGCATTGGATCGA+CGG | - | chr1.3:61400053-61400072 | MS.gene070209:CDS | 45.0% | |
| CAATAGCACCAACACGAACA+AGG | + | chr1.3:61399980-61399999 | None:intergenic | 45.0% | |
| CATACAACCCACGTTATACG+AGG | - | chr1.3:61399796-61399815 | MS.gene070209:CDS | 45.0% | |
| TAGGCTGCAGCACCTATTAA+TGG | + | chr1.3:61399771-61399790 | None:intergenic | 45.0% | |
| ! | CAATGATAATGCAGTCCAGC+TGG | - | chr1.3:61400229-61400248 | MS.gene070209:CDS | 45.0% |
| ! | CTTTGGAAAAGCTGTCTGCT+AGG | - | chr1.3:61400264-61400283 | MS.gene070209:CDS | 45.0% |
| !! | GGCGCATCAGAAGTACTTAT+TGG | - | chr1.3:61399879-61399898 | MS.gene070209:CDS | 45.0% |
| CGTATTGCATTCAAGGAGGC+TGG | - | chr1.3:61400173-61400192 | MS.gene070209:CDS | 50.0% | |
| GCAGCACCTATTAATGGCAC+AGG | + | chr1.3:61399765-61399784 | None:intergenic | 50.0% | |
| TCTGTGCCATCTGGAAATGC+AGG | + | chr1.3:61399824-61399843 | None:intergenic | 50.0% | |
| !! | CAATGCAAGAGTGTCAGACG+CGG | + | chr1.3:61400047-61400066 | None:intergenic | 50.0% |
| CGCGTCTGACACTCTTGCAT+TGG | - | chr1.3:61400045-61400064 | MS.gene070209:CDS | 55.0% | |
| TCAAGGAGGCTGGTGCAGTT+AGG | - | chr1.3:61400183-61400202 | MS.gene070209:CDS | 55.0% | |
| GCAGGCGCCTCGTATAACGT+GGG | + | chr1.3:61399806-61399825 | None:intergenic | 60.0% | |
| TGCAGGCGCCTCGTATAACG+TGG | + | chr1.3:61399807-61399826 | None:intergenic | 60.0% | |
| ! | GAGGCGCCTGCATTTCCAGA+TGG | - | chr1.3:61399815-61399834 | MS.gene070209:CDS | 60.0% |
| AAGCTTGCGTCGCTGCCAGC+TGG | + | chr1.3:61400247-61400266 | None:intergenic | 65.0% | |
| GCTGGCAGCGACGCAAGCTT+TGG | - | chr1.3:61400247-61400266 | MS.gene070209:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61399639 | 61400285 | 61399639 | ID=MS.gene070209 |
| chr1.3 | mRNA | 61399639 | 61400285 | 61399639 | ID=MS.gene070209.t1;Parent=MS.gene070209 |
| chr1.3 | exon | 61399639 | 61400285 | 61399639 | ID=MS.gene070209.t1.exon1;Parent=MS.gene070209.t1 |
| chr1.3 | CDS | 61399639 | 61400285 | 61399639 | ID=cds.MS.gene070209.t1;Parent=MS.gene070209.t1 |
| Gene Sequence |
| Protein sequence |
