Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070639.t1 | XP_003621245.2 | 99.1 | 217 | 2 | 0 | 1 | 217 | 1 | 217 | 1.30E-113 | 419.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070639.t1 | P0C8Q9 | 54.1 | 98 | 43 | 1 | 120 | 217 | 150 | 245 | 1.7e-21 | 104.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070639.t1 | G7L200 | 99.1 | 217 | 2 | 0 | 1 | 217 | 1 | 217 | 9.1e-114 | 419.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060937 | MS.gene070639 | 0.802484 | 5.49E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070639.t1 | MTR_7g010930 | 99.078 | 217 | 2 | 0 | 1 | 217 | 1 | 217 | 4.99e-159 | 438 |
| MS.gene070639.t1 | MTR_6g012390 | 64.186 | 215 | 62 | 2 | 18 | 217 | 1 | 215 | 6.95e-95 | 276 |
| MS.gene070639.t1 | MTR_2g021720 | 42.574 | 101 | 46 | 2 | 120 | 217 | 103 | 194 | 6.48e-16 | 73.2 |
| MS.gene070639.t1 | MTR_2g021720 | 42.574 | 101 | 46 | 2 | 120 | 217 | 103 | 194 | 1.16e-15 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070639.t1 | AT3G01170 | 51.339 | 224 | 93 | 5 | 1 | 217 | 1 | 215 | 6.89e-67 | 205 |
| MS.gene070639.t1 | AT5G15260 | 51.261 | 238 | 91 | 8 | 1 | 217 | 1 | 234 | 8.96e-67 | 205 |
| MS.gene070639.t1 | AT5G15260 | 51.261 | 238 | 91 | 8 | 1 | 217 | 1 | 234 | 8.96e-67 | 205 |
| MS.gene070639.t1 | AT5G19025 | 54.082 | 98 | 43 | 1 | 120 | 217 | 150 | 245 | 9.27e-26 | 100 |
| MS.gene070639.t1 | AT5G19025 | 53.608 | 97 | 43 | 1 | 120 | 216 | 150 | 244 | 4.13e-25 | 99.4 |
| MS.gene070639.t1 | AT3G06180 | 53.061 | 98 | 44 | 1 | 120 | 217 | 132 | 227 | 7.08e-25 | 98.2 |
| MS.gene070639.t1 | AT3G01170 | 39.216 | 102 | 56 | 2 | 1 | 97 | 1 | 101 | 2.12e-11 | 60.1 |
Find 51 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAGCTGTTGTTATTGCTTT+AGG | 0.138895 | 7.1:+84528801 | MS.gene070639:CDS |
| CAAACTTTGTGGTTTGTTTA+TGG | 0.153027 | 7.1:+84528869 | MS.gene070639:CDS |
| TTGATCTTTCTATTCTTCTT+TGG | 0.201359 | 7.1:-84529263 | None:intergenic |
| TGTTATTGCTTTAGGGTTCT+TGG | 0.201503 | 7.1:+84528809 | MS.gene070639:CDS |
| TAGCTGTTGTTATTGCTTTA+GGG | 0.217546 | 7.1:+84528802 | MS.gene070639:CDS |
| TTCTTCAATCCCTTACAATT+AGG | 0.224249 | 7.1:-84529014 | None:intergenic |
| GAATAGAAAGATCAAGAAAT+AGG | 0.263550 | 7.1:+84529271 | MS.gene070639:CDS |
| CTCATCACAAATTGAATATA+TGG | 0.264029 | 7.1:-84528837 | None:intergenic |
| TCTTCAATCCCTTACAATTA+GGG | 0.277107 | 7.1:-84529013 | None:intergenic |
| TGTTGGTAGGTTAGAAGTTC+CGG | 0.283392 | 7.1:+84529238 | MS.gene070639:CDS |
| AAGAAGATGGCTCCTGTTAA+TGG | 0.295596 | 7.1:+84529176 | MS.gene070639:CDS |
| AGATGGTGGTGGAATTAAAA+AGG | 0.303114 | 7.1:+84529103 | MS.gene070639:CDS |
| TTGTGGTTTGTTTATGGATT+TGG | 0.314324 | 7.1:+84528875 | MS.gene070639:CDS |
| CATGTGCTGTTGTTGCTACA+TGG | 0.391115 | 7.1:+84528955 | MS.gene070639:CDS |
| GTTGGTAGGTTAGAAGTTCC+GGG | 0.396075 | 7.1:+84529239 | MS.gene070639:CDS |
| AGTGAAAGTTTCAAACTTTG+TGG | 0.397466 | 7.1:+84528858 | MS.gene070639:CDS |
| CCTGTGATTTATGTATCTCT+TGG | 0.405703 | 7.1:+84528921 | MS.gene070639:CDS |
| AGAACAGCTCTTCCATTAAC+AGG | 0.409782 | 7.1:-84529188 | None:intergenic |
| ATGTGGGAACCCTAATTGTA+AGG | 0.424650 | 7.1:+84529004 | MS.gene070639:CDS |
| TGTGAAGAATTCGCCGTCGT+TGG | 0.440660 | 7.1:+84529076 | MS.gene070639:CDS |
| AGAGCTGTTCTAGTTCTTAG+AGG | 0.448409 | 7.1:+84529200 | MS.gene070639:CDS |
| GTGAAGAATTCGCCGTCGTT+GGG | 0.455176 | 7.1:+84529077 | MS.gene070639:CDS |
| AGTGTGGTTGTTCTGTTGGT+AGG | 0.472839 | 7.1:+84529225 | MS.gene070639:CDS |
| CAACGGATGAGATCAAACTA+TGG | 0.476169 | 7.1:-84528650 | None:intergenic |
| GGAAAGTGTGGTTGTTCTGT+TGG | 0.477218 | 7.1:+84529221 | MS.gene070639:CDS |
| GAAGAGAATTGAGTGTCTTA+CGG | 0.499754 | 7.1:-84528725 | None:intergenic |
| TCGATCCGCGGTTGTTGATG+TGG | 0.500037 | 7.1:+84528770 | MS.gene070639:CDS |
| CTTATACATCAAGGAAATGT+GGG | 0.514031 | 7.1:+84528988 | MS.gene070639:CDS |
| TTTCTATTCTTCTTTGGACC+CGG | 0.518411 | 7.1:-84529257 | None:intergenic |
| CGAAGCCGAGCTTAAGAAGA+TGG | 0.524432 | 7.1:+84529163 | MS.gene070639:CDS |
| AGGAGCCATCTTCTTAAGCT+CGG | 0.536872 | 7.1:-84529168 | None:intergenic |
| TCGCCGTCGTTGGGTAAAGA+TGG | 0.539719 | 7.1:+84529086 | MS.gene070639:CDS |
| GCTTATACATCAAGGAAATG+TGG | 0.544775 | 7.1:+84528987 | MS.gene070639:CDS |
| TGTGGGAACCCTAATTGTAA+GGG | 0.554639 | 7.1:+84529005 | MS.gene070639:CDS |
| TCGCCATGGATGATGATGAT+TGG | 0.560084 | 7.1:-84528674 | None:intergenic |
| TGCTGAGTTTGATATTCAGT+TGG | 0.575183 | 7.1:+84529040 | MS.gene070639:CDS |
| CTAGTTCTTAGAGGAAAGTG+TGG | 0.579987 | 7.1:+84529209 | MS.gene070639:CDS |
| TCCTCGATTTAGCTAAATCG+AGG | 0.583685 | 7.1:-84528701 | None:intergenic |
| TGACCAATCATCATCATCCA+TGG | 0.587614 | 7.1:+84528671 | MS.gene070639:CDS |
| CCAAGAGATACATAAATCAC+AGG | 0.588766 | 7.1:-84528921 | None:intergenic |
| CCCTCGATTTAGCTAAATCG+AGG | 0.592975 | 7.1:+84528700 | MS.gene070639:CDS |
| CCGTCGTTGGGTAAAGATGG+TGG | 0.619641 | 7.1:+84529089 | MS.gene070639:CDS |
| CCTCGATTTAGCTAAATCGA+GGG | 0.621163 | 7.1:-84528700 | None:intergenic |
| GATCGAGATCGCTCGCACTG+CGG | 0.651752 | 7.1:-84528753 | None:intergenic |
| TCGTTGGGTAAAGATGGTGG+TGG | 0.659209 | 7.1:+84529092 | MS.gene070639:CDS |
| AGTTCGCGATGATGATCACA+AGG | 0.663527 | 7.1:-84529140 | None:intergenic |
| GTGCGAGCGATCTCGATCCG+CGG | 0.666573 | 7.1:+84528758 | MS.gene070639:CDS |
| GGATGATGATGATTGGTCAA+CGG | 0.679462 | 7.1:-84528667 | None:intergenic |
| TAAATCGAGGGAATTCGCCA+TGG | 0.679775 | 7.1:-84528688 | None:intergenic |
| CCACCATCTTTACCCAACGA+CGG | 0.694775 | 7.1:-84529089 | None:intergenic |
| TATAACCACATCAACAACCG+CGG | 0.698235 | 7.1:-84528775 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATAGCTTATACATCA+AGG | + | chr7.1:84528979-84528998 | MS.gene070639:CDS | 20.0% |
| ! | CTCATCACAAATTGAATATA+TGG | - | chr7.1:84528840-84528859 | None:intergenic | 25.0% |
| ! | TTGATCTTTCTATTCTTCTT+TGG | - | chr7.1:84529266-84529285 | None:intergenic | 25.0% |
| AGTGAAAGTTTCAAACTTTG+TGG | + | chr7.1:84528858-84528877 | MS.gene070639:CDS | 30.0% | |
| CTTATACATCAAGGAAATGT+GGG | + | chr7.1:84528988-84529007 | MS.gene070639:CDS | 30.0% | |
| TCTTCAATCCCTTACAATTA+GGG | - | chr7.1:84529016-84529035 | None:intergenic | 30.0% | |
| TTCTTCAATCCCTTACAATT+AGG | - | chr7.1:84529017-84529036 | None:intergenic | 30.0% | |
| ! | ATAGCTGTTGTTATTGCTTT+AGG | + | chr7.1:84528801-84528820 | MS.gene070639:CDS | 30.0% |
| ! | TAGCTGTTGTTATTGCTTTA+GGG | + | chr7.1:84528802-84528821 | MS.gene070639:CDS | 30.0% |
| !! | CAAACTTTGTGGTTTGTTTA+TGG | + | chr7.1:84528869-84528888 | MS.gene070639:CDS | 30.0% |
| !! | TTGTGGTTTGTTTATGGATT+TGG | + | chr7.1:84528875-84528894 | MS.gene070639:CDS | 30.0% |
| CCAAGAGATACATAAATCAC+AGG | - | chr7.1:84528924-84528943 | None:intergenic | 35.0% | |
| CCTGTGATTTATGTATCTCT+TGG | + | chr7.1:84528921-84528940 | MS.gene070639:CDS | 35.0% | |
| GAAGAGAATTGAGTGTCTTA+CGG | - | chr7.1:84528728-84528747 | None:intergenic | 35.0% | |
| GCTTATACATCAAGGAAATG+TGG | + | chr7.1:84528987-84529006 | MS.gene070639:CDS | 35.0% | |
| TGCTGAGTTTGATATTCAGT+TGG | + | chr7.1:84529040-84529059 | MS.gene070639:CDS | 35.0% | |
| TTTCTATTCTTCTTTGGACC+CGG | - | chr7.1:84529260-84529279 | None:intergenic | 35.0% | |
| !! | AGATGGTGGTGGAATTAAAA+AGG | + | chr7.1:84529103-84529122 | MS.gene070639:CDS | 35.0% |
| !! | GATGGTGGTGGAATTAAAAA+GGG | + | chr7.1:84529104-84529123 | MS.gene070639:CDS | 35.0% |
| !! | TGTTATTGCTTTAGGGTTCT+TGG | + | chr7.1:84528809-84528828 | MS.gene070639:CDS | 35.0% |
| AAGAAGATGGCTCCTGTTAA+TGG | + | chr7.1:84529176-84529195 | MS.gene070639:CDS | 40.0% | |
| AGAACAGCTCTTCCATTAAC+AGG | - | chr7.1:84529191-84529210 | None:intergenic | 40.0% | |
| ATGTGGGAACCCTAATTGTA+AGG | + | chr7.1:84529004-84529023 | MS.gene070639:CDS | 40.0% | |
| CAACGGATGAGATCAAACTA+TGG | - | chr7.1:84528653-84528672 | None:intergenic | 40.0% | |
| CCTCGATTTAGCTAAATCGA+GGG | - | chr7.1:84528720-84528701 | None:intergenic | 40.0% | |
| TATAACCACATCAACAACCG+CGG | - | chr7.1:84528778-84528797 | None:intergenic | 40.0% | |
| TCCTCGATTTAGCTAAATCG+AGG | - | chr7.1:84528704-84528723 | None:intergenic | 40.0% | |
| TGACCAATCATCATCATCCA+TGG | + | chr7.1:84528671-84528690 | MS.gene070639:CDS | 40.0% | |
| TGTGGGAACCCTAATTGTAA+GGG | + | chr7.1:84529005-84529024 | MS.gene070639:CDS | 40.0% | |
| ! | CTAGTTCTTAGAGGAAAGTG+TGG | + | chr7.1:84529209-84529228 | MS.gene070639:CDS | 40.0% |
| ! | GGATGATGATGATTGGTCAA+CGG | - | chr7.1:84528670-84528689 | None:intergenic | 40.0% |
| ! | TGTTGGTAGGTTAGAAGTTC+CGG | + | chr7.1:84529238-84529257 | MS.gene070639:CDS | 40.0% |
| !! | AGAGCTGTTCTAGTTCTTAG+AGG | + | chr7.1:84529200-84529219 | MS.gene070639:CDS | 40.0% |
| AGGAGCCATCTTCTTAAGCT+CGG | - | chr7.1:84529171-84529190 | None:intergenic | 45.0% | |
| AGTGTGGTTGTTCTGTTGGT+AGG | + | chr7.1:84529225-84529244 | MS.gene070639:CDS | 45.0% | |
| AGTTCGCGATGATGATCACA+AGG | - | chr7.1:84529143-84529162 | None:intergenic | 45.0% | |
| CCCTCGATTTAGCTAAATCG+AGG | + | chr7.1:84528700-84528719 | MS.gene070639:CDS | 45.0% | |
| TAAATCGAGGGAATTCGCCA+TGG | - | chr7.1:84528691-84528710 | None:intergenic | 45.0% | |
| ! | CATGTGCTGTTGTTGCTACA+TGG | + | chr7.1:84528955-84528974 | MS.gene070639:CDS | 45.0% |
| ! | GGAAAGTGTGGTTGTTCTGT+TGG | + | chr7.1:84529221-84529240 | MS.gene070639:CDS | 45.0% |
| ! | GTTGGTAGGTTAGAAGTTCC+GGG | + | chr7.1:84529239-84529258 | MS.gene070639:CDS | 45.0% |
| ! | TCGCCATGGATGATGATGAT+TGG | - | chr7.1:84528677-84528696 | None:intergenic | 45.0% |
| CCACCATCTTTACCCAACGA+CGG | - | chr7.1:84529092-84529111 | None:intergenic | 50.0% | |
| CGAAGCCGAGCTTAAGAAGA+TGG | + | chr7.1:84529163-84529182 | MS.gene070639:CDS | 50.0% | |
| GTGAAGAATTCGCCGTCGTT+GGG | + | chr7.1:84529077-84529096 | MS.gene070639:CDS | 50.0% | |
| TGTGAAGAATTCGCCGTCGT+TGG | + | chr7.1:84529076-84529095 | MS.gene070639:CDS | 50.0% | |
| ! | TCGTTGGGTAAAGATGGTGG+TGG | + | chr7.1:84529092-84529111 | MS.gene070639:CDS | 50.0% |
| CCGTCGTTGGGTAAAGATGG+TGG | + | chr7.1:84529089-84529108 | MS.gene070639:CDS | 55.0% | |
| TCGATCCGCGGTTGTTGATG+TGG | + | chr7.1:84528770-84528789 | MS.gene070639:CDS | 55.0% | |
| TCGCCGTCGTTGGGTAAAGA+TGG | + | chr7.1:84529086-84529105 | MS.gene070639:CDS | 55.0% | |
| GATCGAGATCGCTCGCACTG+CGG | - | chr7.1:84528756-84528775 | None:intergenic | 60.0% | |
| GTGCGAGCGATCTCGATCCG+CGG | + | chr7.1:84528758-84528777 | MS.gene070639:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 84528639 | 84529292 | 84528639 | ID=MS.gene070639 |
| chr7.1 | mRNA | 84528639 | 84529292 | 84528639 | ID=MS.gene070639.t1;Parent=MS.gene070639 |
| chr7.1 | exon | 84528639 | 84529292 | 84528639 | ID=MS.gene070639.t1.exon1;Parent=MS.gene070639.t1 |
| chr7.1 | CDS | 84528639 | 84529292 | 84528639 | ID=cds.MS.gene070639.t1;Parent=MS.gene070639.t1 |
| Gene Sequence |
| Protein sequence |
