AlfalfaGEDB | MS.gene070747

M. sativa cultivar XinJiangDaYe / MS.gene070747

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070747.t1	GAU31586.1	83.5	103	17	0	2	104	512	614	3.80E-31	144.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070747.t1	C0LGG7	43.4	106	56	2	3	104	535	640	1.1e-15	84.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070747.t1	A0A2Z6MFZ2	83.5	103	17	0	2	104	512	614	2.7e-31	144.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054012	MS.gene070747	0.802432	5.63E-49	-1.69E-46
MS.gene054013	MS.gene070747	0.802959	4.38E-49	-1.69E-46
MS.gene054183	MS.gene070747	0.808796	2.60E-50	-1.69E-46
MS.gene059876	MS.gene070747	0.806235	9.08E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070747.t1	MTR_2g074820	78.431	102	22	0	3	104	71	172	4.28e-55	177
MS.gene070747.t1	MTR_2g074990	79.412	102	21	0	3	104	567	668	5.30e-52	174
MS.gene070747.t1	MTR_2g073650	76.923	104	24	0	1	104	575	678	1.78e-51	173
MS.gene070747.t1	MTR_2g073650	76.923	104	24	0	1	104	575	678	2.40e-51	172
MS.gene070747.t1	MTR_2g075010	77.451	102	23	0	3	104	569	670	1.19e-50	171
MS.gene070747.t1	MTR_2g074980	77.885	104	23	0	1	104	543	646	2.29e-49	167
MS.gene070747.t1	MTR_2g074870	69.608	102	25	1	9	104	165	266	2.88e-44	150
MS.gene070747.t1	MTR_2g075060	75.728	103	25	0	2	104	576	678	4.95e-43	149
MS.gene070747.t1	MTR_2g074840	75.000	104	26	0	1	104	576	679	9.83e-42	145
MS.gene070747.t1	MTR_2g074840	75.000	104	26	0	1	104	576	679	2.12e-41	144
MS.gene070747.t1	MTR_5g083910	69.159	107	28	1	3	104	438	544	3.29e-33	121
MS.gene070747.t1	MTR_5g083910	69.159	107	28	1	3	104	570	676	3.38e-33	121
MS.gene070747.t1	MTR_2g073540	51.852	108	47	2	2	104	335	442	5.28e-31	115
MS.gene070747.t1	MTR_2g073520	50.926	108	48	2	2	104	441	548	3.24e-30	112
MS.gene070747.t1	MTR_2g073520	50.926	108	48	2	2	104	573	680	3.72e-29	109
MS.gene070747.t1	MTR_2g073520	50.926	108	48	2	2	104	573	680	4.06e-29	109
MS.gene070747.t1	MTR_2g073640	51.852	108	47	2	2	104	578	685	7.61e-29	108
MS.gene070747.t1	MTR_8g058250	49.524	105	49	2	2	104	578	680	3.53e-28	107
MS.gene070747.t1	MTR_8g058250	49.524	105	49	2	2	104	482	584	3.55e-28	107
MS.gene070747.t1	MTR_8g058250	49.524	105	49	2	2	104	578	680	4.22e-28	106
MS.gene070747.t1	MTR_2g073600	47.664	107	52	2	2	104	572	678	2.46e-27	104
MS.gene070747.t1	MTR_2g073560	46.729	107	53	2	2	104	548	654	5.90e-27	103
MS.gene070747.t1	MTR_2g075250	50.476	105	48	2	2	104	485	587	1.30e-26	102
MS.gene070747.t1	MTR_2g075250	50.476	105	48	2	2	104	580	682	1.53e-26	102
MS.gene070747.t1	MTR_5g091950	46.296	108	52	1	3	104	496	603	4.44e-26	100
MS.gene070747.t1	MTR_5g091950	46.296	108	52	1	3	104	451	558	4.51e-26	100
MS.gene070747.t1	MTR_5g091950	46.296	108	52	1	3	104	586	693	6.45e-26	100
MS.gene070747.t1	MTR_8g059615	52.336	107	47	1	2	104	549	655	3.77e-23	92.8
MS.gene070747.t1	MTR_8g059615	52.336	107	47	1	2	104	549	655	5.57e-23	92.0
MS.gene070747.t1	MTR_2g073630	49.541	109	49	2	2	104	536	644	8.96e-23	91.7
MS.gene070747.t1	MTR_8g059605	52.336	107	47	1	2	104	371	477	1.95e-22	90.5
MS.gene070747.t1	MTR_8g059605	52.336	107	47	1	2	104	566	672	2.11e-22	90.5
MS.gene070747.t1	MTR_8g087740	40.741	108	58	2	3	104	581	688	1.70e-16	73.6
MS.gene070747.t1	MTR_8g461110	33.333	111	65	2	3	104	596	706	1.87e-13	65.1
MS.gene070747.t1	MTR_8g461110	33.333	111	65	2	3	104	596	706	1.97e-13	65.1
MS.gene070747.t1	MTR_7g056640	42.857	77	40	2	32	104	457	533	7.21e-12	60.5
MS.gene070747.t1	MTR_7g056617	45.205	73	36	2	36	104	446	518	3.53e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070747.t1	AT1G53420	49.057	106	50	2	3	104	535	640	7.64e-27	103
MS.gene070747.t1	AT3G14840	50.442	113	46	2	2	104	576	688	2.93e-26	101
MS.gene070747.t1	AT1G07650	47.222	108	50	4	2	104	563	668	1.80e-24	96.7
MS.gene070747.t1	AT1G07650	47.222	108	50	4	2	104	376	481	2.14e-24	96.3
MS.gene070747.t1	AT1G07650	47.222	108	50	4	2	104	589	694	2.15e-24	96.3
MS.gene070747.t1	AT1G07650	47.222	108	50	4	2	104	595	700	2.15e-24	96.3
MS.gene070747.t1	AT1G53430	50.909	110	43	4	3	104	579	685	2.93e-24	95.9
MS.gene070747.t1	AT1G53430	50.909	110	43	4	3	104	538	644	2.98e-24	95.9
MS.gene070747.t1	AT1G53440	50.000	110	44	4	3	104	577	683	9.69e-24	94.4
MS.gene070747.t1	AT1G29750	42.991	107	56	1	3	104	576	682	2.79e-21	87.4
MS.gene070747.t1	AT1G29750	42.991	107	56	1	3	104	591	697	3.17e-21	87.0
MS.gene070747.t1	AT1G29750	42.991	107	56	1	3	104	591	697	3.74e-21	87.0
MS.gene070747.t1	AT1G56130	34.234	111	64	1	3	104	401	511	6.74e-17	74.7
MS.gene070747.t1	AT1G56130	34.234	111	64	1	3	104	452	562	9.24e-17	74.3
MS.gene070747.t1	AT1G56130	34.234	111	64	1	3	104	600	710	1.20e-16	74.3
MS.gene070747.t1	AT1G56140	34.234	111	64	1	3	104	599	709	4.17e-16	72.8
MS.gene070747.t1	AT1G56145	37.391	115	55	2	3	104	593	703	8.82e-16	71.6
MS.gene070747.t1	AT1G56145	37.391	115	55	2	3	104	490	600	8.84e-16	71.6
MS.gene070747.t1	AT1G56145	37.391	115	55	2	3	104	593	703	9.52e-16	71.6
MS.gene070747.t1	AT1G56120	32.432	111	66	1	3	104	598	708	3.77e-14	67.0
MS.gene070747.t1	AT1G56120	32.432	111	66	1	3	104	450	560	3.84e-14	67.0
MS.gene070747.t1	AT1G56120	32.432	111	66	1	3	104	616	726	3.88e-14	67.0
MS.gene070747.t1	AT1G29730	41.346	104	31	3	1	104	583	656	4.59e-14	66.6
MS.gene070747.t1	AT1G29730	41.346	104	31	3	1	104	583	656	6.10e-14	66.6
MS.gene070747.t1	AT1G29720	36.036	111	61	3	3	104	580	689	8.44e-14	66.2
MS.gene070747.t1	AT1G29740	31.356	118	56	3	3	104	585	693	5.47e-11	58.2
MS.gene070747.t1	AT1G29740	31.356	118	56	3	3	104	585	693	8.36e-11	57.4

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGACCATATGCTGATTTATT+TGG	0.097984	2.1:-24557836	None:intergenic
TGCTACCTTGATTTGTTTAA+AGG	0.107562	2.1:-24558418	None:intergenic
TCCTCCTTCTCCAATCTTAT+TGG	0.132396	2.1:-24558460	None:intergenic
CTTTGGTGGAAAGGATGTTT+TGG	0.276152	2.1:+24558060	MS.gene070747:CDS
GACCATATGCTGATTTATTT+GGG	0.324084	2.1:-24557835	None:intergenic
TTCGATATTTCCAATAAGAT+TGG	0.328081	2.1:+24558450	MS.gene070747:CDS
GCTCCTGCAGATATGCTTAT+AGG	0.333779	2.1:-24557982	None:intergenic
AAGATTGGAGAAGGAGGATT+TGG	0.341896	2.1:+24558465	MS.gene070747:CDS
AGGCAAATAATCCTGTTTGT+AGG	0.397135	2.1:-24558398	None:intergenic
ATTTGCCTTTAAACAAATCA+AGG	0.459384	2.1:+24558413	MS.gene070747:CDS
TCAGCTATATCAGTGGAATC+TGG	0.476099	2.1:+24557866	MS.gene070747:CDS
ACTGATATAGCTGATATAAG+AGG	0.479651	2.1:-24557857	None:intergenic
GTCCCAAATAAATCAGCATA+TGG	0.486357	2.1:+24557833	MS.gene070747:CDS
AAGAGTTTAGACCTACAAAC+AGG	0.488837	2.1:+24558387	MS.gene070747:CDS
AGGATTTGGTCCTGTGTTCA+AGG	0.493437	2.1:+24558479	MS.gene070747:CDS
TCCAATAAGATTGGAGAAGG+AGG	0.532435	2.1:+24558459	MS.gene070747:CDS
TATAATGATAATAATTGTTG+TGG	0.555165	2.1:-24558025	None:intergenic
ATTTCCAATAAGATTGGAGA+AGG	0.563365	2.1:+24558456	MS.gene070747:CDS
CCACCTATAAGCATATCTGC+AGG	0.573074	2.1:+24557979	MS.gene070747:CDS
ATATGCTGATTTATTTGGGA+CGG	0.574044	2.1:-24557831	None:intergenic
ATGCAAAATACCTTGAACAC+AGG	0.585163	2.1:-24558489	None:intergenic
ATATCTGCAGGAGCTGTGGT+TGG	0.601973	2.1:+24557991	MS.gene070747:CDS
AAGCATATCTGCAGGAGCTG+TGG	0.606854	2.1:+24557987	MS.gene070747:CDS
TCTTATATCAGCTATATCAG+TGG	0.645114	2.1:+24557859	MS.gene070747:CDS
CCTGCAGATATGCTTATAGG+TGG	0.645323	2.1:-24557979	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATTATTTTGTATACTT+TGG	+	chr2.1:24558043-24558062	MS.gene070747:CDS	10.0%
!!	TATAATGATAATAATTGTTG+TGG	-	chr2.1:24558028-24558047	None:intergenic	15.0%
!!	TTTATTAATGTATCATGTTA+TGG	+	chr2.1:24558234-24558253	MS.gene070747:intron	15.0%
!!!	TATTATTTTGTATACTTTGG+TGG	+	chr2.1:24558046-24558065	MS.gene070747:CDS	20.0%
!	AAGAAAAACTCATTAGCAAA+AGG	+	chr2.1:24558084-24558103	MS.gene070747:CDS	25.0%
!	ATTTGCCTTTAAACAAATCA+AGG	+	chr2.1:24558413-24558432	MS.gene070747:CDS	25.0%
!	TTCGATATTTCCAATAAGAT+TGG	+	chr2.1:24558450-24558469	MS.gene070747:CDS	25.0%
	ACTGATATAGCTGATATAAG+AGG	-	chr2.1:24557860-24557879	None:intergenic	30.0%
	ATTTCCAATAAGATTGGAGA+AGG	+	chr2.1:24558456-24558475	MS.gene070747:CDS	30.0%
	TCTTATATCAGCTATATCAG+TGG	+	chr2.1:24557859-24557878	MS.gene070747:CDS	30.0%
!	TTTTGTATACTTTGGTGGAA+AGG	+	chr2.1:24558051-24558070	MS.gene070747:CDS	30.0%
!!	ATATGCTGATTTATTTGGGA+CGG	-	chr2.1:24557834-24557853	None:intergenic	30.0%
!!	GACCATATGCTGATTTATTT+GGG	-	chr2.1:24557838-24557857	None:intergenic	30.0%
!!	TGCTACCTTGATTTGTTTAA+AGG	-	chr2.1:24558421-24558440	None:intergenic	30.0%
	AAGAGTTTAGACCTACAAAC+AGG	+	chr2.1:24558387-24558406	MS.gene070747:CDS	35.0%
	AGCTCTGTTAATTGAAGTAC+AGG	-	chr2.1:24558366-24558385	None:intergenic	35.0%
	AGGCAAATAATCCTGTTTGT+AGG	-	chr2.1:24558401-24558420	None:intergenic	35.0%
	ATCAAGATAAGACAAGTCTC+AGG	-	chr2.1:24558262-24558281	None:intergenic	35.0%
	CGAGATCATAGCATAAGATT+AGG	-	chr2.1:24557912-24557931	None:intergenic	35.0%
	GTCCCAAATAAATCAGCATA+TGG	+	chr2.1:24557833-24557852	MS.gene070747:CDS	35.0%
!!	GGACCATATGCTGATTTATT+TGG	-	chr2.1:24557839-24557858	None:intergenic	35.0%
	AAGATTGGAGAAGGAGGATT+TGG	+	chr2.1:24558465-24558484	MS.gene070747:CDS	40.0%
	ATGCTTATAGGTGGAGAATC+TGG	-	chr2.1:24557973-24557992	None:intergenic	40.0%
	TCAGCTATATCAGTGGAATC+TGG	+	chr2.1:24557866-24557885	MS.gene070747:CDS	40.0%
	TCCAATAAGATTGGAGAAGG+AGG	+	chr2.1:24558459-24558478	MS.gene070747:CDS	40.0%
	TCCTCCTTCTCCAATCTTAT+TGG	-	chr2.1:24558463-24558482	None:intergenic	40.0%
!	CTTTGGTGGAAAGGATGTTT+TGG	+	chr2.1:24558060-24558079	MS.gene070747:CDS	40.0%
	CCACCTATAAGCATATCTGC+AGG	+	chr2.1:24557979-24557998	MS.gene070747:CDS	45.0%
	CCTGCAGATATGCTTATAGG+TGG	-	chr2.1:24557982-24558001	None:intergenic	45.0%
	GCTCCTGCAGATATGCTTAT+AGG	-	chr2.1:24557985-24558004	None:intergenic	45.0%
	AAGCATATCTGCAGGAGCTG+TGG	+	chr2.1:24557987-24558006	MS.gene070747:CDS	50.0%
	ATATCTGCAGGAGCTGTGGT+TGG	+	chr2.1:24557991-24558010	MS.gene070747:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	24557827	24558500	24557827	ID=MS.gene070747
chr2.1	mRNA	24557827	24558500	24557827	ID=MS.gene070747.t1;Parent=MS.gene070747
chr2.1	exon	24557827	24557887	24557827	ID=MS.gene070747.t1.exon1;Parent=MS.gene070747.t1
chr2.1	CDS	24557827	24557887	24557827	ID=cds.MS.gene070747.t1;Parent=MS.gene070747.t1
chr2.1	exon	24557974	24558105	24557974	ID=MS.gene070747.t1.exon2;Parent=MS.gene070747.t1
chr2.1	CDS	24557974	24558105	24557974	ID=cds.MS.gene070747.t1;Parent=MS.gene070747.t1
chr2.1	exon	24558382	24558500	24558382	ID=MS.gene070747.t1.exon3;Parent=MS.gene070747.t1
chr2.1	CDS	24558382	24558500	24558382	ID=cds.MS.gene070747.t1;Parent=MS.gene070747.t1

Gene Sequence

>MS.gene070747

ATGGCCGTCCCAAATAAATCAGCATATGGTCCTCTTATATCAGCTATATCAGTGGAATCTGGTAAGTTATACTGTGTACATTCCTAATCTTATGCTATGATCTCGATGTTATGAATATTTAAAATGTCTTGTATAATAATCATCCAGATTCTCCACCTATAAGCATATCTGCAGGAGCTGTGGTTGGAATTGTGATTGCCACAACAATTATTATCATTATATTATTTTGTATACTTTGGTGGAAAGGATGTTTTGGAAAGAAAAACTCATTAGCAAAAGGTGATTACAACTTAATTTATTCTGCTATCATTATTATATATCAAACTTTTAGCTAATGCGTTAATTATTCAACTGATAACAAATAAACTTGCGCATGCTGTGTAATTGAGTTACTTTGAGCATGTACTTTTATTAATGTATCATGTTATGGAACCTGAGACTTGTCTTATCTTGATAAATAGAAAGTTGCATGCTTACAAGAAATCGATTGTCGATGCAGACTGTATATATGCATGTATAAGAGAATTTTGATCTTTCCTGTACTTCAATTAACAGAGCTGAAGAGTTTAGACCTACAAACAGGATTATTTGCCTTTAAACAAATCAAGGTAGCAACAAATAACTTCGATATTTCCAATAAGATTGGAGAAGGAGGATTTGGTCCTGTGTTCAAG

Protein sequence

>MS.gene070747.t1

MAVPNKSAYGPLISAISVESDSPPISISAGAVVGIVIATTIIIIILFCILWWKGCFGKKNSLAKELKSLDLQTGLFAFKQIKVATNNFDISNKIGEGGFGPVFK