Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07098.t1 | XP_003626026.1 | 88.2 | 204 | 22 | 1 | 1 | 204 | 1 | 202 | 5.80E-92 | 347.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07098.t1 | Q94K41 | 59.3 | 204 | 80 | 1 | 1 | 204 | 1 | 201 | 8.2e-63 | 241.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07098.t1 | G7KTR4 | 88.2 | 204 | 22 | 1 | 1 | 204 | 1 | 202 | 4.2e-92 | 347.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050430 | MS.gene07098 | 0.850309 | 1.85E-60 | -1.69E-46 |
| MS.gene053974 | MS.gene07098 | 0.801417 | 9.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07098.t1 | MTR_7g110130 | 88.235 | 204 | 22 | 1 | 1 | 204 | 1 | 202 | 1.26e-128 | 363 |
| MS.gene07098.t1 | MTR_7g110160 | 86.700 | 203 | 6 | 1 | 1 | 203 | 1 | 182 | 1.43e-121 | 344 |
| MS.gene07098.t1 | MTR_2g090165 | 82.843 | 204 | 32 | 1 | 1 | 204 | 1 | 201 | 1.63e-121 | 345 |
| MS.gene07098.t1 | MTR_7g110140 | 68.137 | 204 | 62 | 1 | 1 | 204 | 1 | 201 | 2.77e-96 | 281 |
| MS.gene07098.t1 | MTR_6g035395 | 58.416 | 202 | 68 | 2 | 3 | 204 | 2 | 187 | 3.80e-76 | 229 |
| MS.gene07098.t1 | MTR_6g023590 | 53.731 | 201 | 90 | 2 | 5 | 204 | 12 | 210 | 7.94e-70 | 214 |
| MS.gene07098.t1 | MTR_6g023610 | 54.271 | 199 | 88 | 2 | 5 | 202 | 12 | 208 | 3.22e-68 | 210 |
| MS.gene07098.t1 | MTR_6g023950 | 54.726 | 201 | 88 | 2 | 5 | 204 | 12 | 210 | 1.67e-67 | 207 |
| MS.gene07098.t1 | MTR_6g023910 | 52.195 | 205 | 95 | 2 | 2 | 205 | 9 | 211 | 1.01e-66 | 206 |
| MS.gene07098.t1 | MTR_6g023990 | 51.980 | 202 | 94 | 2 | 2 | 202 | 10 | 209 | 1.01e-65 | 203 |
| MS.gene07098.t1 | MTR_6g023600 | 51.741 | 201 | 94 | 2 | 5 | 204 | 12 | 210 | 5.50e-64 | 199 |
| MS.gene07098.t1 | MTR_7g071680 | 51.471 | 204 | 97 | 2 | 2 | 205 | 25 | 226 | 3.42e-63 | 198 |
| MS.gene07098.t1 | MTR_6g023600 | 51.244 | 201 | 89 | 3 | 5 | 204 | 12 | 204 | 2.23e-60 | 189 |
| MS.gene07098.t1 | MTR_4g052300 | 48.768 | 203 | 101 | 2 | 2 | 204 | 4 | 203 | 4.64e-60 | 189 |
| MS.gene07098.t1 | MTR_4g052350 | 51.031 | 194 | 92 | 2 | 4 | 197 | 12 | 202 | 1.75e-59 | 187 |
| MS.gene07098.t1 | MTR_4g052340 | 48.756 | 201 | 100 | 2 | 4 | 204 | 12 | 209 | 2.32e-59 | 187 |
| MS.gene07098.t1 | MTR_4g052320 | 49.490 | 196 | 96 | 2 | 5 | 200 | 7 | 199 | 1.04e-57 | 183 |
| MS.gene07098.t1 | MTR_7g083870 | 49.485 | 194 | 95 | 3 | 4 | 197 | 33 | 223 | 3.21e-56 | 180 |
| MS.gene07098.t1 | MTR_7g105590 | 48.691 | 191 | 95 | 2 | 4 | 194 | 6 | 193 | 4.60e-56 | 176 |
| MS.gene07098.t1 | MTR_7g107670 | 45.000 | 200 | 106 | 3 | 4 | 203 | 31 | 226 | 2.23e-54 | 175 |
| MS.gene07098.t1 | MTR_7g105460 | 49.741 | 193 | 94 | 2 | 5 | 197 | 12 | 201 | 1.43e-53 | 172 |
| MS.gene07098.t1 | MTR_4g078630 | 47.291 | 203 | 100 | 3 | 1 | 202 | 14 | 210 | 2.03e-53 | 172 |
| MS.gene07098.t1 | MTR_7g105580 | 45.274 | 201 | 89 | 2 | 4 | 204 | 6 | 185 | 9.28e-52 | 167 |
| MS.gene07098.t1 | MTR_3g113870 | 44.865 | 185 | 98 | 3 | 14 | 198 | 29 | 209 | 5.03e-47 | 154 |
| MS.gene07098.t1 | MTR_3g020670 | 45.128 | 195 | 103 | 3 | 4 | 197 | 14 | 205 | 1.01e-43 | 147 |
| MS.gene07098.t1 | MTR_4g084800 | 48.227 | 141 | 71 | 1 | 4 | 144 | 11 | 149 | 5.17e-40 | 134 |
| MS.gene07098.t1 | MTR_6g023940 | 35.885 | 209 | 61 | 5 | 2 | 205 | 9 | 149 | 2.59e-29 | 107 |
| MS.gene07098.t1 | MTR_7g105570 | 35.500 | 200 | 71 | 5 | 2 | 197 | 13 | 158 | 7.12e-25 | 97.4 |
| MS.gene07098.t1 | MTR_4g058840 | 33.493 | 209 | 110 | 5 | 1 | 190 | 1 | 199 | 4.65e-22 | 90.9 |
| MS.gene07098.t1 | MTR_4g058820 | 33.493 | 209 | 110 | 5 | 1 | 190 | 1 | 199 | 4.65e-22 | 90.9 |
| MS.gene07098.t1 | MTR_4g058810 | 32.057 | 209 | 113 | 5 | 1 | 190 | 1 | 199 | 9.72e-19 | 82.0 |
| MS.gene07098.t1 | MTR_3g085740 | 25.743 | 202 | 138 | 5 | 4 | 200 | 73 | 267 | 7.60e-18 | 80.5 |
| MS.gene07098.t1 | MTR_4g064887 | 29.703 | 202 | 128 | 5 | 2 | 197 | 11 | 204 | 7.39e-17 | 76.3 |
| MS.gene07098.t1 | MTR_4g064887 | 29.703 | 202 | 128 | 5 | 2 | 197 | 11 | 204 | 1.18e-16 | 76.3 |
| MS.gene07098.t1 | MTR_1g051165 | 28.856 | 201 | 124 | 4 | 4 | 190 | 12 | 207 | 2.28e-15 | 73.2 |
| MS.gene07098.t1 | MTR_7g105560 | 62.500 | 56 | 21 | 0 | 4 | 59 | 11 | 66 | 2.91e-15 | 70.5 |
| MS.gene07098.t1 | MTR_4g086400 | 28.846 | 208 | 117 | 5 | 8 | 195 | 6 | 202 | 6.20e-14 | 70.1 |
| MS.gene07098.t1 | MTR_4g086410 | 29.524 | 210 | 117 | 5 | 8 | 197 | 6 | 204 | 1.16e-13 | 69.3 |
| MS.gene07098.t1 | MTR_7g113650 | 24.880 | 209 | 140 | 4 | 5 | 203 | 39 | 240 | 2.29e-13 | 67.8 |
| MS.gene07098.t1 | MTR_4g131740 | 25.616 | 203 | 135 | 5 | 5 | 197 | 39 | 235 | 3.90e-13 | 67.0 |
| MS.gene07098.t1 | MTR_1g082760 | 26.471 | 204 | 133 | 4 | 5 | 198 | 39 | 235 | 4.10e-13 | 67.0 |
| MS.gene07098.t1 | MTR_3g068175 | 30.198 | 202 | 131 | 6 | 1 | 198 | 12 | 207 | 5.82e-12 | 63.5 |
| MS.gene07098.t1 | MTR_4g097510 | 26.042 | 192 | 135 | 4 | 7 | 197 | 3 | 188 | 1.10e-11 | 62.8 |
| MS.gene07098.t1 | MTR_7g076660 | 36.082 | 97 | 58 | 2 | 9 | 101 | 8 | 104 | 9.51e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07098.t1 | AT2G47140 | 59.314 | 204 | 80 | 1 | 1 | 204 | 1 | 201 | 2.36e-84 | 250 |
| MS.gene07098.t1 | AT2G47130 | 55.000 | 200 | 87 | 1 | 1 | 200 | 1 | 197 | 5.91e-75 | 226 |
| MS.gene07098.t1 | AT3G29260 | 56.345 | 197 | 83 | 1 | 1 | 197 | 1 | 194 | 1.98e-74 | 225 |
| MS.gene07098.t1 | AT3G29250 | 55.838 | 197 | 84 | 1 | 1 | 197 | 1 | 194 | 1.26e-73 | 223 |
| MS.gene07098.t1 | AT3G29250 | 56.410 | 195 | 82 | 1 | 1 | 195 | 37 | 228 | 2.37e-73 | 224 |
| MS.gene07098.t1 | AT2G47120 | 54.315 | 197 | 86 | 2 | 1 | 197 | 1 | 193 | 2.17e-72 | 220 |
| MS.gene07098.t1 | AT3G29250 | 55.959 | 193 | 82 | 1 | 3 | 195 | 41 | 230 | 2.35e-72 | 221 |
| MS.gene07098.t1 | AT2G47120 | 54.404 | 193 | 84 | 2 | 5 | 197 | 6 | 194 | 1.82e-71 | 218 |
| MS.gene07098.t1 | AT3G51680 | 49.029 | 206 | 101 | 1 | 1 | 202 | 27 | 232 | 7.84e-60 | 189 |
| MS.gene07098.t1 | AT3G26760 | 43.719 | 199 | 109 | 3 | 2 | 200 | 32 | 227 | 1.33e-52 | 171 |
| MS.gene07098.t1 | AT3G26770 | 45.000 | 200 | 107 | 3 | 4 | 203 | 39 | 235 | 4.06e-50 | 165 |
| MS.gene07098.t1 | AT4G03140 | 47.396 | 192 | 97 | 3 | 4 | 195 | 76 | 263 | 1.63e-49 | 164 |
| MS.gene07098.t1 | AT3G42960 | 48.500 | 200 | 97 | 2 | 2 | 201 | 4 | 197 | 2.30e-48 | 159 |
| MS.gene07098.t1 | AT1G52340 | 44.444 | 198 | 103 | 3 | 4 | 197 | 16 | 210 | 2.11e-45 | 152 |
| MS.gene07098.t1 | AT2G47150 | 37.762 | 143 | 66 | 2 | 6 | 148 | 1 | 120 | 1.18e-22 | 90.9 |
| MS.gene07098.t1 | AT2G29340 | 29.902 | 204 | 135 | 4 | 3 | 203 | 4 | 202 | 2.75e-17 | 77.0 |
| MS.gene07098.t1 | AT2G29340 | 29.902 | 204 | 135 | 4 | 3 | 203 | 4 | 202 | 2.75e-17 | 77.0 |
| MS.gene07098.t1 | AT2G29340 | 30.303 | 198 | 130 | 4 | 3 | 197 | 4 | 196 | 8.80e-17 | 76.6 |
| MS.gene07098.t1 | AT2G29340 | 30.303 | 198 | 130 | 4 | 3 | 197 | 4 | 196 | 2.11e-16 | 76.3 |
| MS.gene07098.t1 | AT3G55310 | 31.443 | 194 | 119 | 5 | 6 | 190 | 17 | 205 | 2.60e-16 | 75.5 |
| MS.gene07098.t1 | AT1G62610 | 31.088 | 193 | 122 | 4 | 6 | 190 | 13 | 202 | 2.81e-16 | 75.5 |
| MS.gene07098.t1 | AT1G62610 | 31.088 | 193 | 122 | 4 | 6 | 190 | 14 | 203 | 2.90e-16 | 75.5 |
| MS.gene07098.t1 | AT1G62610 | 31.088 | 193 | 122 | 4 | 6 | 190 | 15 | 204 | 3.18e-16 | 75.5 |
| MS.gene07098.t1 | AT1G62610 | 31.088 | 193 | 122 | 4 | 6 | 190 | 19 | 208 | 3.53e-16 | 75.5 |
| MS.gene07098.t1 | AT3G55290 | 30.729 | 192 | 123 | 4 | 6 | 190 | 18 | 206 | 3.71e-16 | 75.1 |
| MS.gene07098.t1 | AT3G55290 | 30.729 | 192 | 123 | 4 | 6 | 190 | 17 | 205 | 4.06e-16 | 75.1 |
| MS.gene07098.t1 | AT1G63380 | 31.443 | 194 | 120 | 6 | 6 | 190 | 15 | 204 | 2.37e-15 | 73.2 |
| MS.gene07098.t1 | AT1G63380 | 31.443 | 194 | 120 | 6 | 6 | 190 | 19 | 208 | 2.59e-15 | 72.8 |
| MS.gene07098.t1 | AT1G63380 | 31.443 | 194 | 120 | 6 | 6 | 190 | 17 | 206 | 3.07e-15 | 72.8 |
| MS.gene07098.t1 | AT1G63380 | 31.443 | 194 | 120 | 6 | 6 | 190 | 22 | 211 | 3.14e-15 | 72.8 |
| MS.gene07098.t1 | AT1G49670 | 28.910 | 211 | 119 | 5 | 7 | 197 | 5 | 204 | 7.07e-15 | 72.8 |
| MS.gene07098.t1 | AT1G49670 | 28.910 | 211 | 119 | 5 | 7 | 197 | 5 | 204 | 8.00e-15 | 72.8 |
| MS.gene07098.t1 | AT2G29310 | 29.208 | 202 | 127 | 6 | 3 | 197 | 4 | 196 | 9.67e-15 | 70.1 |
| MS.gene07098.t1 | AT2G29290 | 31.481 | 216 | 118 | 6 | 3 | 204 | 4 | 203 | 1.14e-14 | 70.9 |
| MS.gene07098.t1 | AT1G24360 | 25.628 | 199 | 136 | 5 | 4 | 197 | 72 | 263 | 1.82e-14 | 70.9 |
| MS.gene07098.t1 | AT2G29310 | 29.208 | 202 | 127 | 6 | 3 | 197 | 4 | 196 | 2.45e-14 | 70.1 |
| MS.gene07098.t1 | AT2G30670 | 28.500 | 200 | 131 | 5 | 3 | 197 | 4 | 196 | 6.29e-14 | 68.9 |
| MS.gene07098.t1 | AT1G07450 | 29.756 | 205 | 135 | 5 | 1 | 202 | 3 | 201 | 7.09e-14 | 68.6 |
| MS.gene07098.t1 | AT1G07440 | 28.708 | 209 | 119 | 7 | 3 | 197 | 9 | 201 | 1.58e-13 | 67.8 |
| MS.gene07098.t1 | AT2G29350 | 30.476 | 210 | 116 | 8 | 2 | 197 | 11 | 204 | 1.59e-13 | 67.4 |
| MS.gene07098.t1 | AT2G29360 | 30.093 | 216 | 121 | 8 | 2 | 203 | 12 | 211 | 2.03e-13 | 67.4 |
| MS.gene07098.t1 | AT1G54870 | 26.316 | 209 | 137 | 4 | 5 | 203 | 83 | 284 | 3.60e-13 | 67.4 |
| MS.gene07098.t1 | AT1G54870 | 26.316 | 209 | 137 | 4 | 5 | 203 | 81 | 282 | 3.89e-13 | 67.4 |
| MS.gene07098.t1 | AT2G29350 | 30.476 | 210 | 116 | 8 | 2 | 197 | 11 | 204 | 4.55e-13 | 66.6 |
| MS.gene07098.t1 | AT3G46170 | 29.016 | 193 | 127 | 4 | 5 | 190 | 25 | 214 | 4.66e-13 | 66.6 |
| MS.gene07098.t1 | AT2G17845 | 31.707 | 205 | 107 | 9 | 6 | 190 | 47 | 238 | 7.84e-12 | 63.5 |
| MS.gene07098.t1 | AT2G29260 | 29.680 | 219 | 120 | 8 | 2 | 203 | 62 | 263 | 1.89e-11 | 62.4 |
| MS.gene07098.t1 | AT2G29290 | 32.530 | 166 | 93 | 5 | 42 | 204 | 37 | 186 | 1.96e-11 | 61.6 |
| MS.gene07098.t1 | AT3G05260 | 26.961 | 204 | 131 | 4 | 5 | 198 | 36 | 231 | 2.42e-11 | 62.0 |
| MS.gene07098.t1 | AT3G06060 | 30.653 | 199 | 125 | 4 | 3 | 196 | 51 | 241 | 2.91e-11 | 62.0 |
| MS.gene07098.t1 | AT3G06060 | 31.500 | 200 | 122 | 6 | 3 | 196 | 34 | 224 | 3.02e-11 | 62.0 |
| MS.gene07098.t1 | AT5G19200 | 31.937 | 191 | 115 | 6 | 12 | 196 | 41 | 222 | 8.57e-11 | 60.5 |
Find 46 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTGGCTGGCTGTGTTGC+TGG | 0.229497 | 7.2:+6344423 | MS.gene07098:CDS |
| GTTAATTCAATTTCACCTTC+TGG | 0.256250 | 7.2:+6344537 | MS.gene07098:CDS |
| TTTATGTAGAGCACTGAATA+TGG | 0.259202 | 7.2:+6344575 | MS.gene07098:CDS |
| ACATCCAAACATGGTCTTAT+AGG | 0.260415 | 7.2:+6344468 | MS.gene07098:CDS |
| AGCTGATCCACGAACATTAA+TGG | 0.267172 | 7.2:-6344331 | None:intergenic |
| GCAGATATCAATGATGAATT+TGG | 0.272072 | 7.2:+6344096 | MS.gene07098:CDS |
| GTAAAGACATTTGTGGAAAA+TGG | 0.275212 | 7.2:+6344057 | MS.gene07098:CDS |
| CACCAAGTTGCAACTTCAAT+TGG | 0.283127 | 7.2:+6344120 | MS.gene07098:CDS |
| TGCAGTGAGCTTGGAGCTTA+TGG | 0.289846 | 7.2:+6344507 | MS.gene07098:CDS |
| GGCTGTGTTGCTGGATGTGC+AGG | 0.325612 | 7.2:+6344432 | MS.gene07098:CDS |
| CAAGCACGCTGCGCGTGTTA+TGG | 0.373393 | 7.2:+6344362 | MS.gene07098:CDS |
| AAGGCCTATAAGACCATGTT+TGG | 0.388328 | 7.2:-6344472 | None:intergenic |
| CGCTCGACGTGCAGTGAGCT+TGG | 0.402697 | 7.2:+6344498 | MS.gene07098:CDS |
| ATTGCTGAACATGATGTCTA+AGG | 0.405158 | 7.2:-6344232 | None:intergenic |
| ACTGGTGGAGCAAGCGGAAT+AGG | 0.405788 | 7.2:+6344024 | MS.gene07098:CDS |
| GACATCATGTTCAGCAATGT+TGG | 0.440291 | 7.2:+6344237 | MS.gene07098:CDS |
| ACATCATGTTCAGCAATGTT+GGG | 0.442033 | 7.2:+6344238 | MS.gene07098:CDS |
| TTATGTAGAGCACTGAATAT+GGG | 0.444828 | 7.2:+6344576 | MS.gene07098:CDS |
| ACCAAGTTGCAACTTCAATT+GGG | 0.462862 | 7.2:+6344121 | MS.gene07098:CDS |
| ATTTGCACGGCCAGTGTGGC+TGG | 0.477946 | 7.2:+6344411 | MS.gene07098:CDS |
| CACTGCACGTCGAGCGAACA+AGG | 0.479956 | 7.2:-6344491 | None:intergenic |
| ATGTTCAGCAATGTTGGGAT+TGG | 0.487016 | 7.2:+6344243 | MS.gene07098:CDS |
| TTCAGCAATGTTGGGATTGG+AGG | 0.491721 | 7.2:+6344246 | MS.gene07098:CDS |
| ACCATGGCCATTAATGTTCG+TGG | 0.509551 | 7.2:+6344324 | MS.gene07098:CDS |
| ACCCAATTGAAGTTGCAACT+TGG | 0.520892 | 7.2:-6344122 | None:intergenic |
| CAATTGAAGTTGCAACTTGG+TGG | 0.525100 | 7.2:-6344119 | None:intergenic |
| TTCAGTGCTCTACATAAAAG+AGG | 0.531671 | 7.2:-6344570 | None:intergenic |
| AGAGACAGTAAAGACATTTG+TGG | 0.542046 | 7.2:+6344050 | MS.gene07098:CDS |
| GGTAAGGTTGCTATAGTGAC+TGG | 0.551471 | 7.2:+6344006 | MS.gene07098:CDS |
| AGCAACACAGCCAGCCACAC+TGG | 0.557849 | 7.2:-6344421 | None:intergenic |
| TATGTAGAGCACTGAATATG+GGG | 0.559108 | 7.2:+6344577 | MS.gene07098:CDS |
| GCACGCTGCGCGTGTTATGG+TGG | 0.567971 | 7.2:+6344365 | MS.gene07098:CDS |
| TCCACGAACATTAATGGCCA+TGG | 0.568390 | 7.2:-6344325 | None:intergenic |
| TCGTGGATCAATAATTTGCA+CGG | 0.577556 | 7.2:+6344398 | MS.gene07098:CDS |
| AGAGGTGTGGCAACTCCAGA+AGG | 0.591710 | 7.2:-6344552 | None:intergenic |
| AATAATTTGCACGGCCAGTG+TGG | 0.607708 | 7.2:+6344407 | MS.gene07098:CDS |
| GAATAATCATGTCAAAACAA+AGG | 0.611671 | 7.2:+6343840 | None:intergenic |
| TTTCATCTCTCACGTCACAA+TGG | 0.615719 | 7.2:-6344164 | None:intergenic |
| ATAGTGACTGGTGGAGCAAG+CGG | 0.615867 | 7.2:+6344018 | MS.gene07098:CDS |
| CATCTCTCACGTCACAATGG+TGG | 0.625388 | 7.2:-6344161 | None:intergenic |
| TGCTCTACATAAAAGAGGTG+TGG | 0.627277 | 7.2:-6344565 | None:intergenic |
| AAGGTTGCTATAGTGACTGG+TGG | 0.636382 | 7.2:+6344009 | MS.gene07098:CDS |
| ATGTAGAGCACTGAATATGG+GGG | 0.636447 | 7.2:+6344578 | MS.gene07098:CDS |
| GATTATACAACATCCAAACA+TGG | 0.670645 | 7.2:+6344459 | MS.gene07098:CDS |
| AAATGAATTTGACAACACCA+TGG | 0.693926 | 7.2:+6344308 | MS.gene07098:CDS |
| TGTAGAGCACTGAATATGGG+GGG | 0.706314 | 7.2:+6344579 | MS.gene07098:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAATACTATATTTTTTCAT+AGG | + | chr7.2:6343977-6343996 | MS.gene07098:intron | 15.0% |
| !! | AAATATGTATATACACTACT+AGG | - | chr7.2:6343938-6343957 | None:intergenic | 20.0% |
| !!! | GTGTATATACATATTTCTAT+TGG | + | chr7.2:6343942-6343961 | MS.gene07098:intron | 20.0% |
| !!! | TACTATATTTTTTCATAGGT+TGG | + | chr7.2:6343981-6344000 | MS.gene07098:intron | 20.0% |
| !!! | TTTCTACCTTTTTCTTTTTT+TGG | + | chr7.2:6343895-6343914 | MS.gene07098:intron | 20.0% |
| ! | GTTTGTCCAAAAAAAGAAAA+AGG | - | chr7.2:6343904-6343923 | None:intergenic | 25.0% |
| !!! | ATATTTTTTCATAGGTTGGA+GGG | + | chr7.2:6343985-6344004 | MS.gene07098:intron | 25.0% |
| !!! | TATATTTTTTCATAGGTTGG+AGG | + | chr7.2:6343984-6344003 | MS.gene07098:intron | 25.0% |
| AAATGAATTTGACAACACCA+TGG | + | chr7.2:6344308-6344327 | MS.gene07098:CDS | 30.0% | |
| AGAAAAAGGTAGAAAGAAAG+AGG | - | chr7.2:6343890-6343909 | None:intergenic | 30.0% | |
| GAAAAAGGTAGAAAGAAAGA+GGG | - | chr7.2:6343889-6343908 | None:intergenic | 30.0% | |
| GATTATACAACATCCAAACA+TGG | + | chr7.2:6344459-6344478 | MS.gene07098:CDS | 30.0% | |
| GCAGATATCAATGATGAATT+TGG | + | chr7.2:6344096-6344115 | MS.gene07098:CDS | 30.0% | |
| GTAAAGACATTTGTGGAAAA+TGG | + | chr7.2:6344057-6344076 | MS.gene07098:CDS | 30.0% | |
| GTTAATTCAATTTCACCTTC+TGG | + | chr7.2:6344537-6344556 | MS.gene07098:CDS | 30.0% | |
| ! | GCTTTTGCATTGGAAAAATA+TGG | + | chr7.2:6344207-6344226 | MS.gene07098:CDS | 30.0% |
| ! | TTTTTCCAATGCAAAAGCTA+CGG | - | chr7.2:6344205-6344224 | None:intergenic | 30.0% |
| !! | TTATGTAGAGCACTGAATAT+GGG | + | chr7.2:6344576-6344595 | MS.gene07098:CDS | 30.0% |
| !! | TTTATGTAGAGCACTGAATA+TGG | + | chr7.2:6344575-6344594 | MS.gene07098:CDS | 30.0% |
| ACATCATGTTCAGCAATGTT+GGG | + | chr7.2:6344238-6344257 | MS.gene07098:CDS | 35.0% | |
| ACCAAGTTGCAACTTCAATT+GGG | + | chr7.2:6344121-6344140 | MS.gene07098:CDS | 35.0% | |
| AGAGACAGTAAAGACATTTG+TGG | + | chr7.2:6344050-6344069 | MS.gene07098:CDS | 35.0% | |
| ATTGCTGAACATGATGTCTA+AGG | - | chr7.2:6344235-6344254 | None:intergenic | 35.0% | |
| GAGAGATGAAAAACAAGTTG+AGG | + | chr7.2:6344176-6344195 | MS.gene07098:CDS | 35.0% | |
| TTCAGTGCTCTACATAAAAG+AGG | - | chr7.2:6344573-6344592 | None:intergenic | 35.0% | |
| ! | ATGGTGGAAAGAAAAATTCG+TGG | + | chr7.2:6344381-6344400 | MS.gene07098:CDS | 35.0% |
| ! | TCGTGGATCAATAATTTGCA+CGG | + | chr7.2:6344398-6344417 | MS.gene07098:CDS | 35.0% |
| !! | ACATCCAAACATGGTCTTAT+AGG | + | chr7.2:6344468-6344487 | MS.gene07098:CDS | 35.0% |
| !! | TATGTAGAGCACTGAATATG+GGG | + | chr7.2:6344577-6344596 | MS.gene07098:CDS | 35.0% |
| AAGGCCTATAAGACCATGTT+TGG | - | chr7.2:6344475-6344494 | None:intergenic | 40.0% | |
| ACCCAATTGAAGTTGCAACT+TGG | - | chr7.2:6344125-6344144 | None:intergenic | 40.0% | |
| AGCTGATCCACGAACATTAA+TGG | - | chr7.2:6344334-6344353 | None:intergenic | 40.0% | |
| ATGTTCAGCAATGTTGGGAT+TGG | + | chr7.2:6344243-6344262 | MS.gene07098:CDS | 40.0% | |
| CAATTGAAGTTGCAACTTGG+TGG | - | chr7.2:6344122-6344141 | None:intergenic | 40.0% | |
| CACCAAGTTGCAACTTCAAT+TGG | + | chr7.2:6344120-6344139 | MS.gene07098:CDS | 40.0% | |
| GACATCATGTTCAGCAATGT+TGG | + | chr7.2:6344237-6344256 | MS.gene07098:CDS | 40.0% | |
| TGCTCTACATAAAAGAGGTG+TGG | - | chr7.2:6344568-6344587 | None:intergenic | 40.0% | |
| TTTCATCTCTCACGTCACAA+TGG | - | chr7.2:6344167-6344186 | None:intergenic | 40.0% | |
| !! | ATGTAGAGCACTGAATATGG+GGG | + | chr7.2:6344578-6344597 | MS.gene07098:CDS | 40.0% |
| !! | TTTTCATAGGTTGGAGGGTA+AGG | + | chr7.2:6343990-6344009 | MS.gene07098:intron | 40.0% |
| AAGGTTGCTATAGTGACTGG+TGG | + | chr7.2:6344009-6344028 | MS.gene07098:CDS | 45.0% | |
| ACCATGGCCATTAATGTTCG+TGG | + | chr7.2:6344324-6344343 | MS.gene07098:CDS | 45.0% | |
| GGTAAGGTTGCTATAGTGAC+TGG | + | chr7.2:6344006-6344025 | MS.gene07098:CDS | 45.0% | |
| TCCACGAACATTAATGGCCA+TGG | - | chr7.2:6344328-6344347 | None:intergenic | 45.0% | |
| TTCAGCAATGTTGGGATTGG+AGG | + | chr7.2:6344246-6344265 | MS.gene07098:CDS | 45.0% | |
| ! | AATAATTTGCACGGCCAGTG+TGG | + | chr7.2:6344407-6344426 | MS.gene07098:CDS | 45.0% |
| ! | GGAAACCGTAGCTTTTGCAT+TGG | + | chr7.2:6344197-6344216 | MS.gene07098:CDS | 45.0% |
| ATAGTGACTGGTGGAGCAAG+CGG | + | chr7.2:6344018-6344037 | MS.gene07098:CDS | 50.0% | |
| CATCTCTCACGTCACAATGG+TGG | - | chr7.2:6344164-6344183 | None:intergenic | 50.0% | |
| TGCAGTGAGCTTGGAGCTTA+TGG | + | chr7.2:6344507-6344526 | MS.gene07098:CDS | 50.0% | |
| ACTGGTGGAGCAAGCGGAAT+AGG | + | chr7.2:6344024-6344043 | MS.gene07098:CDS | 55.0% | |
| ! | AGAGGTGTGGCAACTCCAGA+AGG | - | chr7.2:6344555-6344574 | None:intergenic | 55.0% |
| AGCAACACAGCCAGCCACAC+TGG | - | chr7.2:6344424-6344443 | None:intergenic | 60.0% | |
| ATTTGCACGGCCAGTGTGGC+TGG | + | chr7.2:6344411-6344430 | MS.gene07098:CDS | 60.0% | |
| CAAGCACGCTGCGCGTGTTA+TGG | + | chr7.2:6344362-6344381 | MS.gene07098:CDS | 60.0% | |
| CACTGCACGTCGAGCGAACA+AGG | - | chr7.2:6344494-6344513 | None:intergenic | 60.0% | |
| ! | AGTGTGGCTGGCTGTGTTGC+TGG | + | chr7.2:6344423-6344442 | MS.gene07098:CDS | 60.0% |
| ! | GGCTGTGTTGCTGGATGTGC+AGG | + | chr7.2:6344432-6344451 | MS.gene07098:CDS | 60.0% |
| CGCTCGACGTGCAGTGAGCT+TGG | + | chr7.2:6344498-6344517 | MS.gene07098:CDS | 65.0% | |
| GCACGCTGCGCGTGTTATGG+TGG | + | chr7.2:6344365-6344384 | MS.gene07098:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 6343848 | 6344600 | 6343848 | ID=MS.gene07098 |
| chr7.2 | mRNA | 6343848 | 6344600 | 6343848 | ID=MS.gene07098.t1;Parent=MS.gene07098 |
| chr7.2 | exon | 6343848 | 6343861 | 6343848 | ID=MS.gene07098.t1.exon1;Parent=MS.gene07098.t1 |
| chr7.2 | CDS | 6343848 | 6343861 | 6343848 | ID=cds.MS.gene07098.t1;Parent=MS.gene07098.t1 |
| chr7.2 | exon | 6343999 | 6344600 | 6343999 | ID=MS.gene07098.t1.exon2;Parent=MS.gene07098.t1 |
| chr7.2 | CDS | 6343999 | 6344600 | 6343999 | ID=cds.MS.gene07098.t1;Parent=MS.gene07098.t1 |
| Gene Sequence |
| Protein sequence |
