AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070990.t1 MTR_1g102350 98.715 389 5 0 1 389 394 782 0.0 793
MS.gene070990.t1 MTR_2g042130 52.320 388 174 3 2 385 394 774 5.21e-135 404
MS.gene070990.t1 MTR_4g125180 50.773 388 176 5 4 385 375 753 5.00e-129 388
MS.gene070990.t1 MTR_4g095360 49.482 386 188 3 4 385 374 756 1.49e-128 387
MS.gene070990.t1 MTR_4g073540 51.688 385 178 4 12 389 390 773 1.72e-127 385
MS.gene070990.t1 MTR_8g022870 50.258 388 182 4 13 389 394 781 1.18e-122 373
MS.gene070990.t1 MTR_7g075900 48.677 378 186 4 13 385 399 773 8.19e-119 362
MS.gene070990.t1 MTR_8g021130 48.947 380 184 5 14 385 399 776 1.57e-116 357
MS.gene070990.t1 MTR_5g016120 49.067 375 186 4 14 385 390 762 1.31e-113 349
MS.gene070990.t1 MTR_3g102810 48.092 393 191 5 3 385 367 756 2.24e-112 345
MS.gene070990.t1 MTR_1g018510 46.753 385 192 4 13 385 344 727 2.99e-110 339
MS.gene070990.t1 MTR_0047s0120 47.733 375 191 4 14 385 386 758 2.37e-107 332
MS.gene070990.t1 MTR_3g082200 43.316 374 200 7 13 383 267 631 9.52e-100 309
MS.gene070990.t1 MTR_3g082200 43.316 374 200 7 13 383 282 646 2.13e-99 309
MS.gene070990.t1 MTR_3g082200 42.670 382 207 7 5 383 397 769 2.35e-98 309
MS.gene070990.t1 MTR_7g098630 46.736 383 186 8 13 383 384 760 2.41e-98 311
MS.gene070990.t1 MTR_4g134320 43.700 373 199 6 12 383 403 765 4.44e-98 308
MS.gene070990.t1 MTR_8g072440 45.000 380 188 6 7 383 349 710 8.47e-98 306
MS.gene070990.t1 MTR_8g072440 45.000 380 188 6 7 383 391 752 1.18e-97 307
MS.gene070990.t1 MTR_5g055920 45.213 376 197 6 13 385 299 668 1.41e-96 302
MS.gene070990.t1 MTR_4g103495 44.416 385 203 8 3 383 246 623 7.36e-96 299
MS.gene070990.t1 MTR_7g098070 42.627 373 203 6 12 383 399 761 9.36e-96 302
MS.gene070990.t1 MTR_4g103480 45.213 376 197 6 13 385 419 788 4.78e-95 301
MS.gene070990.t1 MTR_4g103490 45.238 378 194 7 13 385 416 785 7.71e-95 300
MS.gene070990.t1 MTR_5g025010 44.474 380 192 7 7 383 354 717 5.64e-94 297
MS.gene070990.t1 MTR_5g025010 44.474 380 192 7 7 383 386 749 6.53e-94 297
MS.gene070990.t1 MTR_4g103450 44.681 376 199 6 13 385 376 745 1.50e-92 293
MS.gene070990.t1 MTR_4g102400 44.103 390 179 11 12 385 388 754 8.31e-91 289
MS.gene070990.t1 MTR_4g103430 43.733 375 202 6 13 384 401 769 3.63e-89 285
MS.gene070990.t1 MTR_5g011340 43.342 383 194 6 11 385 385 752 4.20e-86 276
MS.gene070990.t1 MTR_5g011320 42.932 382 196 5 11 385 381 747 4.07e-85 274
MS.gene070990.t1 MTR_3g061110 40.104 384 221 8 4 385 378 754 2.21e-84 272
MS.gene070990.t1 MTR_5g011310 42.559 383 197 6 11 385 388 755 9.69e-84 270
MS.gene070990.t1 MTR_5g085690 42.602 392 205 10 4 383 405 788 3.78e-83 270
MS.gene070990.t1 MTR_4g053630 41.176 374 205 9 12 381 288 650 3.82e-83 266
MS.gene070990.t1 MTR_5g011190 43.264 386 192 10 11 386 388 756 7.90e-83 268
MS.gene070990.t1 MTR_8g042670 43.351 376 197 9 14 383 268 633 1.13e-82 265
MS.gene070990.t1 MTR_4g103400 41.538 390 211 11 4 383 394 776 1.89e-82 268
MS.gene070990.t1 MTR_4g053630 41.176 374 205 9 12 381 365 727 2.57e-82 266
MS.gene070990.t1 MTR_5g011270 40.816 392 209 7 2 385 377 753 3.26e-82 266
MS.gene070990.t1 MTR_4g103495 45.373 335 175 7 3 336 395 722 8.07e-82 266
MS.gene070990.t1 MTR_8g042670 43.351 376 197 9 14 383 392 757 2.97e-81 264
MS.gene070990.t1 MTR_2g018135 40.945 381 206 6 14 386 416 785 4.75e-81 264
MS.gene070990.t1 MTR_2g018125 40.731 383 208 6 12 386 415 786 8.31e-81 263
MS.gene070990.t1 MTR_5g011370 42.857 385 193 10 11 385 408 775 8.78e-81 263
MS.gene070990.t1 MTR_5g011260 40.204 393 212 7 2 386 377 754 2.64e-80 261
MS.gene070990.t1 MTR_7g081420 41.071 392 213 11 3 383 400 784 4.26e-80 261
MS.gene070990.t1 MTR_8g024390 39.801 402 212 13 1 384 369 758 4.57e-80 261
MS.gene070990.t1 MTR_7g081750 39.901 406 212 11 3 383 399 797 5.90e-80 261
MS.gene070990.t1 MTR_7g081580 41.885 382 204 10 13 383 409 783 2.86e-79 259
MS.gene070990.t1 MTR_8g042710 42.857 371 201 8 14 379 395 759 4.61e-79 258
MS.gene070990.t1 MTR_4g053635 39.678 373 209 8 12 381 311 670 4.44e-77 251
MS.gene070990.t1 MTR_8g024420 38.710 403 215 13 1 384 333 722 1.20e-76 251
MS.gene070990.t1 MTR_1g078110 37.629 388 213 11 13 386 384 756 2.20e-76 251
MS.gene070990.t1 MTR_8g024380 38.046 389 214 12 13 386 385 761 7.60e-76 249
MS.gene070990.t1 MTR_5g011280 38.832 394 217 7 2 386 380 758 9.22e-76 249
MS.gene070990.t1 MTR_7g481600 42.147 382 203 10 13 383 409 783 2.15e-74 247
MS.gene070990.t1 MTR_7g081380 39.563 412 209 14 3 383 404 806 3.95e-74 246
MS.gene070990.t1 MTR_1g079120 39.005 382 217 8 12 383 385 760 9.59e-74 244
MS.gene070990.t1 MTR_7g081500 39.295 397 212 11 3 383 399 782 1.22e-73 244
MS.gene070990.t1 MTR_8g083220 39.370 381 212 7 7 383 409 774 2.90e-73 243
MS.gene070990.t1 MTR_5g096750 38.889 378 205 8 13 383 406 764 1.54e-72 241
MS.gene070990.t1 MTR_7g079300 38.630 365 209 9 28 383 407 765 4.33e-72 240
MS.gene070990.t1 MTR_2g049200 36.968 376 215 8 20 384 392 756 5.82e-72 239
MS.gene070990.t1 MTR_7g079310 36.458 384 223 9 11 383 390 763 6.73e-71 237
MS.gene070990.t1 MTR_8g005710 38.727 377 198 12 19 386 349 701 2.54e-69 231
MS.gene070990.t1 MTR_3g104930 38.903 383 216 11 12 384 388 762 1.46e-68 230
MS.gene070990.t1 MTR_7g034380 39.247 372 197 12 19 383 342 691 2.39e-68 228
MS.gene070990.t1 MTR_8g033150 37.887 388 215 10 5 387 361 727 2.57e-67 226
MS.gene070990.t1 MTR_8g033150 37.887 388 215 10 5 387 388 754 4.14e-67 226
MS.gene070990.t1 MTR_3g114420 38.764 356 199 9 40 386 422 767 8.33e-67 226
MS.gene070990.t1 MTR_1g006990 41.223 376 187 15 20 386 387 737 1.31e-66 224
MS.gene070990.t1 MTR_8g079900 36.719 384 220 9 4 383 279 643 9.17e-66 221
MS.gene070990.t1 MTR_7g118080 37.166 374 209 9 27 386 397 758 1.02e-65 223
MS.gene070990.t1 MTR_1g007030 40.267 375 192 14 20 386 385 735 1.16e-65 222
MS.gene070990.t1 MTR_8g079900 36.719 384 220 9 4 383 377 741 3.57e-65 221
MS.gene070990.t1 MTR_8g027260 41.424 309 153 8 85 383 361 651 1.05e-64 218
MS.gene070990.t1 MTR_7g104200 38.624 378 207 11 12 383 383 741 1.63e-64 219
MS.gene070990.t1 MTR_6g055570 39.096 376 193 14 19 383 379 729 2.55e-63 216
MS.gene070990.t1 MTR_1g048110 38.095 378 196 14 20 386 383 733 4.68e-63 215
MS.gene070990.t1 MTR_7g075890 35.806 391 221 11 4 387 533 900 8.39e-62 214
MS.gene070990.t1 MTR_8g024370 35.396 404 229 11 1 384 366 757 1.48e-61 211
MS.gene070990.t1 MTR_8g006680 36.074 377 202 13 19 387 381 726 2.91e-60 207
MS.gene070990.t1 MTR_8g005300 36.074 377 202 13 19 387 381 726 2.91e-60 207
MS.gene070990.t1 MTR_8g005640 36.074 377 202 13 19 387 381 726 2.91e-60 207
MS.gene070990.t1 MTR_3g464790 38.281 384 201 12 14 384 372 732 9.33e-60 206
MS.gene070990.t1 MTR_3g010080 36.911 382 198 16 19 386 382 734 2.10e-59 205
MS.gene070990.t1 MTR_2g042650 36.757 370 191 12 19 383 311 642 2.21e-59 204
MS.gene070990.t1 MTR_6g075370 42.586 263 139 5 111 372 424 675 4.86e-59 203
MS.gene070990.t1 MTR_6g055410 36.757 370 192 11 19 383 379 711 4.81e-58 201
MS.gene070990.t1 MTR_2g042640 38.369 331 139 13 19 341 332 605 1.18e-49 177
MS.gene070990.t1 MTR_2g044630 40.370 270 135 9 128 383 448 705 3.66e-47 171
MS.gene070990.t1 MTR_3g452290 35.987 314 150 9 87 386 295 571 8.70e-46 166
MS.gene070990.t1 MTR_3g451220 35.987 314 150 9 87 386 295 571 8.70e-46 166
MS.gene070990.t1 MTR_4g127480 37.838 296 152 11 109 387 537 817 2.75e-44 164
MS.gene070990.t1 MTR_2g037850 33.333 399 215 18 19 381 428 811 2.94e-44 164
MS.gene070990.t1 MTR_4g126230 41.827 208 102 5 111 313 55 248 3.51e-42 148
MS.gene070990.t1 MTR_3g114140 34.030 335 188 10 26 344 105 422 4.86e-42 152
MS.gene070990.t1 MTR_8g042710 46.907 194 96 4 14 203 395 585 3.14e-41 154
MS.gene070990.t1 MTR_8g089230 30.296 406 224 16 19 383 458 845 7.16e-39 148
MS.gene070990.t1 MTR_4g132620 35.069 288 163 10 111 387 542 816 1.90e-37 144
MS.gene070990.t1 MTR_3g114410 44.330 194 100 4 13 203 393 581 2.66e-36 139
MS.gene070990.t1 MTR_2g046740 33.448 290 167 10 111 385 562 840 7.21e-35 136
MS.gene070990.t1 MTR_4g071560 41.706 211 105 6 3 203 318 520 2.14e-34 134
MS.gene070990.t1 MTR_4g053665 35.433 254 116 9 130 381 1 208 1.60e-33 124
MS.gene070990.t1 MTR_2g070260 37.931 203 112 7 171 370 1 192 3.70e-33 123
MS.gene070990.t1 MTR_5g044060 47.826 138 67 3 99 233 1 136 1.03e-32 121
MS.gene070990.t1 MTR_3g005755 82.192 73 12 1 161 232 236 308 3.44e-31 120
MS.gene070990.t1 MTR_7g079295 39.130 161 92 3 226 383 352 509 3.24e-27 113
MS.gene070990.t1 MTR_5g081130 35.870 184 106 4 200 383 1 172 1.43e-22 94.7
MS.gene070990.t1 MTR_7g097250 36.250 160 77 4 29 188 42 176 6.91e-22 92.4
MS.gene070990.t1 MTR_8g027660 30.769 195 107 4 195 386 80 249 1.04e-21 93.6
MS.gene070990.t1 MTR_4g118440 28.112 249 127 7 129 374 1 200 1.26e-20 89.7
MS.gene070990.t1 MTR_5g011300 51.613 93 28 3 171 255 1 84 9.83e-20 84.0
MS.gene070990.t1 MTR_7g081660 37.297 185 108 5 201 383 374 552 1.46e-19 90.9
MS.gene070990.t1 MTR_3g114400 31.953 169 91 7 222 384 13 163 9.55e-14 68.9
MS.gene070990.t1 MTR_3g065240 31.868 182 84 5 20 200 81 223 2.46e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070990.t1 AT5G51750 70.769 390 113 1 1 389 391 780 0.0 586
MS.gene070990.t1 AT2G05920 51.948 385 176 3 4 385 373 751 3.44e-134 402
MS.gene070990.t1 AT5G67360 51.429 385 180 4 4 385 376 756 8.38e-126 380
MS.gene070990.t1 AT3G14240 49.105 391 186 4 12 389 385 775 1.57e-124 377
MS.gene070990.t1 AT4G34980 50.524 382 182 3 12 386 380 761 2.97e-123 374
MS.gene070990.t1 AT3G14067 48.438 384 185 6 13 385 388 769 7.91e-114 350
MS.gene070990.t1 AT2G04160 44.909 383 200 7 4 383 396 770 6.19e-102 318
MS.gene070990.t1 AT1G04110 45.550 382 193 6 13 384 395 771 1.96e-99 312
MS.gene070990.t1 AT1G01900 46.316 380 195 5 13 385 396 773 3.62e-99 311
MS.gene070990.t1 AT5G59810 41.823 373 207 6 13 383 410 774 6.09e-88 282
MS.gene070990.t1 AT5G45650 41.361 382 203 5 12 384 420 789 8.46e-84 271
MS.gene070990.t1 AT5G45650 41.361 382 203 5 12 384 420 789 8.46e-84 271
MS.gene070990.t1 AT4G00230 39.583 384 197 11 14 387 388 746 1.31e-72 241
MS.gene070990.t1 AT5G59100 38.501 387 210 11 7 386 375 740 1.45e-71 238
MS.gene070990.t1 AT1G20160 38.947 380 215 10 14 384 352 723 8.45e-71 236
MS.gene070990.t1 AT1G20160 38.947 380 215 10 14 384 391 762 1.28e-70 236
MS.gene070990.t1 AT5G59090 37.209 387 219 9 5 386 364 731 3.03e-69 232
MS.gene070990.t1 AT5G59090 37.209 387 219 9 5 386 362 729 3.23e-69 231
MS.gene070990.t1 AT4G26330 40.179 336 190 6 55 383 390 721 1.51e-68 230
MS.gene070990.t1 AT4G26330 40.179 336 190 6 55 383 404 735 1.93e-68 229
MS.gene070990.t1 AT5G59090 37.468 387 213 10 5 386 364 726 1.23e-67 227
MS.gene070990.t1 AT5G59120 37.500 384 216 9 5 383 363 727 2.29e-67 226
MS.gene070990.t1 AT1G20150 38.918 388 209 12 14 384 395 771 1.09e-66 226
MS.gene070990.t1 AT5G58840 36.559 372 204 8 17 383 362 706 8.96e-66 222
MS.gene070990.t1 AT5G59190 38.259 379 209 11 14 388 336 693 5.62e-65 219
MS.gene070990.t1 AT3G46850 39.433 388 205 13 3 383 370 734 5.98e-63 215
MS.gene070990.t1 AT5G58820 40.199 301 161 5 88 383 414 700 1.09e-62 213
MS.gene070990.t1 AT5G67090 36.927 371 203 9 10 373 370 716 1.81e-62 213
MS.gene070990.t1 AT5G58830 44.643 280 135 6 111 383 439 705 2.77e-62 213
MS.gene070990.t1 AT1G32970 39.394 363 198 9 27 383 376 722 4.85e-60 207
MS.gene070990.t1 AT4G10520 38.462 364 205 8 23 383 397 744 5.41e-60 207
MS.gene070990.t1 AT3G46840 38.032 376 205 13 13 383 382 734 9.12e-60 206
MS.gene070990.t1 AT5G59130 36.461 373 210 12 14 383 338 686 2.17e-59 204
MS.gene070990.t1 AT5G59130 36.461 373 210 12 14 383 373 721 4.46e-59 204
MS.gene070990.t1 AT5G59130 36.461 373 210 12 14 383 377 725 4.54e-59 204
MS.gene070990.t1 AT5G03620 37.150 393 206 12 12 383 386 758 1.05e-58 204
MS.gene070990.t1 AT1G32950 36.997 373 206 10 21 383 357 710 1.81e-58 202
MS.gene070990.t1 AT1G32950 36.997 373 206 10 21 383 408 761 4.98e-58 202
MS.gene070990.t1 AT4G10510 36.709 395 211 11 7 383 321 694 1.12e-57 200
MS.gene070990.t1 AT4G10510 36.709 395 211 11 7 383 336 709 1.29e-57 200
MS.gene070990.t1 AT1G32960 38.315 368 204 10 23 383 414 765 1.86e-57 200
MS.gene070990.t1 AT5G59130 36.729 373 199 13 14 383 377 715 2.26e-57 199
MS.gene070990.t1 AT1G32940 37.228 368 212 8 21 383 358 711 3.78e-57 199
MS.gene070990.t1 AT1G32940 37.228 368 212 8 21 383 358 711 3.78e-57 199
MS.gene070990.t1 AT4G10510 36.962 395 210 12 7 383 380 753 4.36e-57 199
MS.gene070990.t1 AT5G45640 35.171 381 225 8 13 384 385 752 4.55e-57 199
MS.gene070990.t1 AT4G10510 36.962 395 210 12 7 383 392 765 4.60e-57 199
MS.gene070990.t1 AT4G10550 37.097 372 206 10 21 383 358 710 8.59e-57 198
MS.gene070990.t1 AT4G10550 37.097 372 206 10 21 383 358 710 8.59e-57 198
MS.gene070990.t1 AT5G11940 36.915 363 210 8 26 383 404 752 8.64e-57 198
MS.gene070990.t1 AT1G32940 37.330 367 211 8 22 383 410 762 1.38e-56 198
MS.gene070990.t1 AT4G10550 37.097 372 206 10 21 383 414 766 3.05e-56 197
MS.gene070990.t1 AT4G10550 37.097 372 206 10 21 383 430 782 3.12e-56 197
MS.gene070990.t1 AT1G66220 33.693 371 223 7 15 383 395 744 4.77e-55 193
MS.gene070990.t1 AT4G21650 36.657 341 193 7 50 383 436 760 2.03e-54 192
MS.gene070990.t1 AT4G10540 36.290 372 210 11 21 383 410 763 8.11e-54 190
MS.gene070990.t1 AT1G66210 35.342 365 208 8 25 383 408 750 6.59e-52 185
MS.gene070990.t1 AT4G10530 34.699 366 207 7 21 383 399 735 1.05e-51 184
MS.gene070990.t1 AT4G21630 36.765 340 194 8 50 383 429 753 1.54e-51 184
MS.gene070990.t1 AT4G21640 38.762 307 169 8 82 383 435 727 7.23e-51 182
MS.gene070990.t1 AT4G21323 34.783 345 202 10 43 381 464 791 3.53e-49 177
MS.gene070990.t1 AT4G21326 33.243 370 223 9 20 383 336 687 5.43e-47 171
MS.gene070990.t1 AT4G30020 30.361 415 245 13 1 382 407 810 1.23e-45 167
MS.gene070990.t1 AT4G30020 30.361 415 245 13 1 382 407 810 1.23e-45 167
MS.gene070990.t1 AT4G30020 30.361 415 245 13 1 382 407 810 1.23e-45 167
MS.gene070990.t1 AT4G30020 30.361 415 245 13 1 382 407 810 1.23e-45 167
MS.gene070990.t1 AT4G15040 33.933 389 222 11 3 383 324 685 2.93e-45 166
MS.gene070990.t1 AT4G15040 33.933 389 222 11 3 383 324 685 2.93e-45 166
MS.gene070990.t1 AT2G19170 36.552 290 155 9 109 382 533 809 6.42e-44 163
MS.gene070990.t1 AT2G19170 36.552 290 155 9 109 382 533 809 6.42e-44 163
MS.gene070990.t1 AT1G62340 32.170 401 219 15 19 383 446 829 3.66e-41 155
MS.gene070990.t1 AT1G30600 30.597 402 237 14 18 385 435 828 1.47e-39 150
MS.gene070990.t1 AT2G39850 34.708 291 157 11 112 383 489 765 1.51e-39 150
MS.gene070990.t1 AT5G44530 30.750 400 230 16 19 383 446 833 2.93e-39 149
MS.gene070990.t1 AT1G32980 42.781 187 101 3 167 352 3 184 8.92e-39 137
MS.gene070990.t1 AT4G00230 41.969 193 98 4 14 203 388 569 7.20e-38 144
MS.gene070990.t1 AT4G20430 31.281 406 226 20 17 384 459 849 2.66e-36 141
MS.gene070990.t1 AT4G20430 31.281 406 226 20 17 384 459 849 2.66e-36 141
MS.gene070990.t1 AT4G20430 35.052 291 158 13 111 384 549 825 8.21e-36 139
MS.gene070990.t1 AT5G44530 29.176 425 229 18 19 383 446 858 5.06e-35 137
MS.gene070990.t1 AT5G58840 40.107 187 96 3 17 203 362 532 1.48e-33 131
MS.gene070990.t1 AT5G03620 40.816 196 107 3 12 203 386 576 1.50e-31 126
MS.gene070990.t1 AT1G32940 39.086 197 107 5 22 214 410 597 9.95e-24 103
MS.gene070990.t1 AT5G59110 35.185 162 98 3 227 383 7 166 1.89e-23 96.3

Find 63 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATTGTTACACCATTTAA+AGG 0.178184 1.4:-79179681 MS.gene070990:CDS
GCTGCTTGGAGTGGACTTAC+TGG 0.235422 1.4:-79180218 MS.gene070990:CDS
CACACCAAACCTCCAAATTC+TGG 0.235592 1.4:+79179558 None:intergenic
TGTAACAATGACATGATATT+TGG 0.274740 1.4:+79179692 None:intergenic
TTAACAACAGCGCCTTTAAA+TGG 0.275106 1.4:+79179669 None:intergenic
CTTGTAAAATTCAATGTATC+TGG 0.289773 1.4:+79179639 None:intergenic
AGGCAAAGTGAACCAGAATT+TGG 0.295595 1.4:-79179570 MS.gene070990:CDS
TTAACCCTTCTATGATCTAT+TGG 0.295681 1.4:+79180182 None:intergenic
GTGAATCGTCGAAGCAAAAT+TGG 0.296323 1.4:+79179746 None:intergenic
GTTAAGTTTAACATACTTTC+CGG 0.306723 1.4:-79180164 MS.gene070990:CDS
ATAGCAGCTGGACTCCATTC+AGG 0.318680 1.4:+79180083 None:intergenic
GTTTACCAATAGATCATAGA+AGG 0.337442 1.4:-79180187 MS.gene070990:CDS
ACACCAGGTGCTACTATATC+AGG 0.364343 1.4:+79180251 None:intergenic
GATGAAAATGCTGCTACTAT+AGG 0.369103 1.4:+79180311 None:intergenic
TGAACCAGAATTTGGAGGTT+TGG 0.376243 1.4:-79179562 MS.gene070990:CDS
TGATCTATTGGTAAACTTGA+TGG 0.377526 1.4:+79180194 None:intergenic
GAGGTTTGGTGTGGAAAGAT+AGG 0.390179 1.4:-79179548 MS.gene070990:CDS
AGGACATGACATTGAAGTTC+CGG 0.408580 1.4:+79180145 None:intergenic
TGATCCAAGATCTTTGTGTT+TGG 0.414895 1.4:-79180621 MS.gene070990:CDS
CCTTCCAAACACAAAGATCT+TGG 0.423780 1.4:+79180617 None:intergenic
AATTAGTCCAAGAGTGCAAA+AGG 0.429857 1.4:-79180543 MS.gene070990:CDS
TTTGTGTTTGGAAGGTACTT+TGG 0.430850 1.4:-79180609 MS.gene070990:CDS
GCAGAAGAAGCATCTCTAAG+AGG 0.432819 1.4:+79180011 None:intergenic
GGCATATATGTTATCACAAT+CGG 0.437021 1.4:+79179507 None:intergenic
AAGATTGTGATTTGTGATAG+AGG 0.446697 1.4:-79180566 MS.gene070990:CDS
CCTAGAAAAGCTCTTGATCC+AGG 0.449023 1.4:-79179945 MS.gene070990:CDS
TCAGTTTCAAAGTGACATCT+AGG 0.470839 1.4:-79179590 MS.gene070990:CDS
GTGTGAACATTCTTGCTGCT+TGG 0.471231 1.4:-79180232 MS.gene070990:CDS
CTTACAAACACTGCAGCAAA+TGG 0.472737 1.4:-79180482 MS.gene070990:CDS
AACATTCTTGCTGCTTGGAG+TGG 0.477323 1.4:-79180227 MS.gene070990:CDS
ATGTCATGTCCTCATGTAAG+TGG 0.481265 1.4:-79180134 MS.gene070990:CDS
CCAAGATCTTTGTGTTTGGA+AGG 0.493533 1.4:-79180617 MS.gene070990:CDS
AGTATATGATATCGAACCTC+AGG 0.493737 1.4:-79179919 MS.gene070990:CDS
TGGCCAGCACCATGATCATA+AGG 0.495707 1.4:+79179975 None:intergenic
TTGGATCGTCGAACTGTCAC+GGG 0.501662 1.4:-79180590 MS.gene070990:CDS
TCCAAGAGTGCAAAAGGGAC+AGG 0.505100 1.4:-79180537 MS.gene070990:CDS
CACCGCACTGTTACCAATGT+TGG 0.506302 1.4:-79179726 MS.gene070990:CDS
ATTTCAAGTGTGAGTAAACT+AGG 0.508793 1.4:+79180281 None:intergenic
AAACACAAAGATCTTGGATC+AGG 0.511063 1.4:+79180623 None:intergenic
CAGAATTTGGAGGTTTGGTG+TGG 0.515812 1.4:-79179557 MS.gene070990:CDS
CAAAGTGAACCAGAATTTGG+AGG 0.522433 1.4:-79179567 MS.gene070990:CDS
TTTGGATCGTCGAACTGTCA+CGG 0.527993 1.4:-79180591 MS.gene070990:CDS
TCAAAGCTAAACATCCTGAA+TGG 0.530445 1.4:-79180097 MS.gene070990:CDS
ACACCTTATGATCATGGTGC+TGG 0.538752 1.4:-79179978 MS.gene070990:CDS
GGGGAAGAACTTCACATTGG+TGG 0.539133 1.4:+79179486 None:intergenic
AGGTTTGGTGTGGAAAGATA+GGG 0.539280 1.4:-79179547 MS.gene070990:CDS
ATCGTCGAAGCAAAATTGGT+TGG 0.539328 1.4:+79179750 None:intergenic
GGAAACACAACTGATATAGC+AGG 0.548430 1.4:+79179780 None:intergenic
TCGATATCATATACTAAACC+TGG 0.561273 1.4:+79179927 None:intergenic
ACATGATATTTGGAAACAGC+AGG 0.567192 1.4:+79179702 None:intergenic
GGAAACAGCAGGACCAACAT+TGG 0.584506 1.4:+79179713 None:intergenic
CTACTGCTACTGCTGGAAGT+AGG 0.588254 1.4:+79180435 None:intergenic
CTACTTCCAGCAGTAGCAGT+AGG 0.599375 1.4:-79180434 MS.gene070990:CDS
ATTAGTCCAAGAGTGCAAAA+GGG 0.604652 1.4:-79180542 MS.gene070990:CDS
AATGCTGCTACTATAGGAGA+TGG 0.611627 1.4:+79180317 None:intergenic
TTTACCAATAGATCATAGAA+GGG 0.613414 1.4:-79180186 MS.gene070990:CDS
TCCTCATGTAAGTGGAATCG+CGG 0.624644 1.4:-79180126 MS.gene070990:CDS
GCAGCAAGAATGTTCACACC+AGG 0.640405 1.4:+79180236 None:intergenic
AAACCTGATATAGTAGCACC+TGG 0.653822 1.4:-79180254 MS.gene070990:CDS
AGAAACTCAAAGTAATCCTG+AGG 0.669990 1.4:+79179903 None:intergenic
CTAGGGGAAGAACTTCACAT+TGG 0.680242 1.4:+79179483 None:intergenic
GCCGCGATTCCACTTACATG+AGG 0.688521 1.4:+79180125 None:intergenic
GACCAACATTGGTAACAGTG+CGG 0.756399 1.4:+79179724 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACTACTAATTCTGAA+GGG + chr1.4:79180282-79180301 None:intergenic 20.0%
! CTTGTAAAATTCAATGTATC+TGG + chr1.4:79180505-79180524 None:intergenic 25.0%
! GAAAAAACTACTAATTCTGA+AGG + chr1.4:79180283-79180302 None:intergenic 25.0%
! GTTAAGTTTAACATACTTTC+CGG - chr1.4:79179977-79179996 MS.gene070990:CDS 25.0%
! TGTAACAATGACATGATATT+TGG + chr1.4:79180452-79180471 None:intergenic 25.0%
! TTTACCAATAGATCATAGAA+GGG - chr1.4:79179955-79179974 MS.gene070990:CDS 25.0%
GGCATATATGTTATCACAAT+CGG + chr1.4:79180637-79180656 None:intergenic 30.0%
GTCATTGTTACACCATTTAA+AGG - chr1.4:79180460-79180479 MS.gene070990:CDS 30.0%
GTTTACCAATAGATCATAGA+AGG - chr1.4:79179954-79179973 MS.gene070990:CDS 30.0%
TCGATATCATATACTAAACC+TGG + chr1.4:79180217-79180236 None:intergenic 30.0%
TGATCTATTGGTAAACTTGA+TGG + chr1.4:79179950-79179969 None:intergenic 30.0%
TTAACCCTTCTATGATCTAT+TGG + chr1.4:79179962-79179981 None:intergenic 30.0%
! AAGATTGTGATTTGTGATAG+AGG - chr1.4:79179575-79179594 MS.gene070990:CDS 30.0%
! ATTTCAAGTGTGAGTAAACT+AGG + chr1.4:79179863-79179882 None:intergenic 30.0%
! GCTTTTCATAACACAAGATT+AGG - chr1.4:79179794-79179813 MS.gene070990:CDS 30.0%
! TTTTCAACACCTTATGATCA+TGG - chr1.4:79180157-79180176 MS.gene070990:CDS 30.0%
!!! GAGCTTTTCTAGGATTAATA+TGG + chr1.4:79180189-79180208 None:intergenic 30.0%
AAACACAAAGATCTTGGATC+AGG + chr1.4:79179521-79179540 None:intergenic 35.0%
ACATGATATTTGGAAACAGC+AGG + chr1.4:79180442-79180461 None:intergenic 35.0%
AGAAACTCAAAGTAATCCTG+AGG + chr1.4:79180241-79180260 None:intergenic 35.0%
AGTATATGATATCGAACCTC+AGG - chr1.4:79180222-79180241 MS.gene070990:CDS 35.0%
GATGAAAATGCTGCTACTAT+AGG + chr1.4:79179833-79179852 None:intergenic 35.0%
TCAAAGCTAAACATCCTGAA+TGG - chr1.4:79180044-79180063 MS.gene070990:CDS 35.0%
TCAGTTTCAAAGTGACATCT+AGG - chr1.4:79180551-79180570 MS.gene070990:CDS 35.0%
TGATCCAAGATCTTTGTGTT+TGG - chr1.4:79179520-79179539 MS.gene070990:CDS 35.0%
TTAACAACAGCGCCTTTAAA+TGG + chr1.4:79180475-79180494 None:intergenic 35.0%
! AATTAGTCCAAGAGTGCAAA+AGG - chr1.4:79179598-79179617 MS.gene070990:CDS 35.0%
! ATTAGTCCAAGAGTGCAAAA+GGG - chr1.4:79179599-79179618 MS.gene070990:CDS 35.0%
! ATTGCAGATTTTATAGCAGC+TGG + chr1.4:79180073-79180092 None:intergenic 35.0%
! TAGCAGCATTTTCATCAAGA+GGG - chr1.4:79179837-79179856 MS.gene070990:CDS 35.0%
!! GTGCTACTATATCAGGTTTT+AGG + chr1.4:79179886-79179905 None:intergenic 35.0%
!! TTTGTGTTTGGAAGGTACTT+TGG - chr1.4:79179532-79179551 MS.gene070990:CDS 35.0%
AAACCTGATATAGTAGCACC+TGG - chr1.4:79179887-79179906 MS.gene070990:CDS 40.0%
AATGCTGCTACTATAGGAGA+TGG + chr1.4:79179827-79179846 None:intergenic 40.0%
AGGACATGACATTGAAGTTC+CGG + chr1.4:79179999-79180018 None:intergenic 40.0%
AGGCAAAGTGAACCAGAATT+TGG - chr1.4:79180571-79180590 MS.gene070990:CDS 40.0%
ATCGTCGAAGCAAAATTGGT+TGG + chr1.4:79180394-79180413 None:intergenic 40.0%
ATGTCATGTCCTCATGTAAG+TGG - chr1.4:79180007-79180026 MS.gene070990:CDS 40.0%
CAAAGTGAACCAGAATTTGG+AGG - chr1.4:79180574-79180593 MS.gene070990:CDS 40.0%
CCAAGATCTTTGTGTTTGGA+AGG - chr1.4:79179524-79179543 MS.gene070990:CDS 40.0%
CCTTCCAAACACAAAGATCT+TGG + chr1.4:79179527-79179546 None:intergenic 40.0%
CTTACAAACACTGCAGCAAA+TGG - chr1.4:79179659-79179678 MS.gene070990:CDS 40.0%
GGAAACACAACTGATATAGC+AGG + chr1.4:79180364-79180383 None:intergenic 40.0%
GTAGCAGTAGGAGAAAAAGA+AGG - chr1.4:79179719-79179738 MS.gene070990:CDS 40.0%
GTGAATCGTCGAAGCAAAAT+TGG + chr1.4:79180398-79180417 None:intergenic 40.0%
TGAACCAGAATTTGGAGGTT+TGG - chr1.4:79180579-79180598 MS.gene070990:CDS 40.0%
! GTAGCAGCATTTTCATCAAG+AGG - chr1.4:79179836-79179855 MS.gene070990:CDS 40.0%
!! AGGTTTGGTGTGGAAAGATA+GGG - chr1.4:79180594-79180613 MS.gene070990:CDS 40.0%
!!! GCAAAATTGGTTGGTTTTGC+TGG + chr1.4:79180385-79180404 None:intergenic 40.0%
ACACCTTATGATCATGGTGC+TGG - chr1.4:79180163-79180182 MS.gene070990:CDS 45.0%
CACACCAAACCTCCAAATTC+TGG + chr1.4:79180586-79180605 None:intergenic 45.0%
CAGGTAGTGAAAAATGCAGG+AGG - chr1.4:79179623-79179642 MS.gene070990:CDS 45.0%
CCTAGAAAAGCTCTTGATCC+AGG - chr1.4:79180196-79180215 MS.gene070990:CDS 45.0%
GGACAGGTAGTGAAAAATGC+AGG - chr1.4:79179620-79179639 MS.gene070990:CDS 45.0%
GTGTGAACATTCTTGCTGCT+TGG - chr1.4:79179909-79179928 MS.gene070990:CDS 45.0%
TCCTCATGTAAGTGGAATCG+CGG - chr1.4:79180015-79180034 MS.gene070990:CDS 45.0%
TTTGGATCGTCGAACTGTCA+CGG - chr1.4:79179550-79179569 MS.gene070990:CDS 45.0%
! AACATTCTTGCTGCTTGGAG+TGG - chr1.4:79179914-79179933 MS.gene070990:CDS 45.0%
! CCTGGATCAAGAGCTTTTCT+AGG + chr1.4:79180199-79180218 None:intergenic 45.0%
! GACCAACATTGGTAACAGTG+CGG + chr1.4:79180420-79180439 None:intergenic 45.0%
! GTGAAAAATGCAGGAGGTGT+AGG - chr1.4:79179629-79179648 MS.gene070990:CDS 45.0%
! TTTTCTCCTACTGCTACTGC+TGG + chr1.4:79179716-79179735 None:intergenic 45.0%
!! ACACCAGGTGCTACTATATC+AGG + chr1.4:79179893-79179912 None:intergenic 45.0%
!! CAGAATTTGGAGGTTTGGTG+TGG - chr1.4:79180584-79180603 MS.gene070990:CDS 45.0%
!! GAGGTTTGGTGTGGAAAGAT+AGG - chr1.4:79180593-79180612 MS.gene070990:CDS 45.0%
!! GCAGAAGAAGCATCTCTAAG+AGG + chr1.4:79180133-79180152 None:intergenic 45.0%
ATAGCAGCTGGACTCCATTC+AGG + chr1.4:79180061-79180080 None:intergenic 50.0%
CACCGCACTGTTACCAATGT+TGG - chr1.4:79180415-79180434 MS.gene070990:CDS 50.0%
CTACTGCTACTGCTGGAAGT+AGG + chr1.4:79179709-79179728 None:intergenic 50.0%
CTACTTCCAGCAGTAGCAGT+AGG - chr1.4:79179707-79179726 MS.gene070990:CDS 50.0%
GCAGCAAGAATGTTCACACC+AGG + chr1.4:79179908-79179927 None:intergenic 50.0%
GGAAACAGCAGGACCAACAT+TGG + chr1.4:79180431-79180450 None:intergenic 50.0%
TGGCCAGCACCATGATCATA+AGG + chr1.4:79180169-79180188 None:intergenic 50.0%
TTGGATCGTCGAACTGTCAC+GGG - chr1.4:79179551-79179570 MS.gene070990:CDS 50.0%
! ACCTGTCCCTTTTGCACTCT+TGG + chr1.4:79179608-79179627 None:intergenic 50.0%
! TCCAAGAGTGCAAAAGGGAC+AGG - chr1.4:79179604-79179623 MS.gene070990:CDS 50.0%
GCCGCGATTCCACTTACATG+AGG + chr1.4:79180019-79180038 None:intergenic 55.0%
! GCTGCTTGGAGTGGACTTAC+TGG - chr1.4:79179923-79179942 MS.gene070990:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 79179497 79180666 79179497 ID=MS.gene070990
chr1.4 mRNA 79179497 79180666 79179497 ID=MS.gene070990.t1;Parent=MS.gene070990
chr1.4 exon 79179497 79180666 79179497 ID=MS.gene070990.t1.exon1;Parent=MS.gene070990.t1
chr1.4 CDS 79179497 79180666 79179497 ID=cds.MS.gene070990.t1;Parent=MS.gene070990.t1
Gene Sequence

>MS.gene070990

ATGGGAAGCAACTCAAGCAGTCCTGATCCAAGATCTTTGTGTTTGGAAGGTACTTTGGATCGTCGAACTGTCACGGGAAAGATTGTGATTTGTGATAGAGGAATTAGTCCAAGAGTGCAAAAGGGACAGGTAGTGAAAAATGCAGGAGGTGTAGGAATGATTCTTACAAACACTGCAGCAAATGGAGAAGAATTAGTAGCAGATTGTCACCTACTTCCAGCAGTAGCAGTAGGAGAAAAAGAAGGCAAAGATATCAAACAATATGTGTTAACAACTAAAAAAGCTACAGCTACATTAGCTTTTCATAACACAAGATTAGGAATTAGACCATCTCCTATAGTAGCAGCATTTTCATCAAGAGGGCCTAGTTTACTCACACTTGAAATCCTAAAACCTGATATAGTAGCACCTGGTGTGAACATTCTTGCTGCTTGGAGTGGACTTACTGGTCCATCAAGTTTACCAATAGATCATAGAAGGGTTAAGTTTAACATACTTTCCGGAACTTCAATGTCATGTCCTCATGTAAGTGGAATCGCGGCTATGATCAAAGCTAAACATCCTGAATGGAGTCCAGCTGCTATAAAATCTGCAATCATGACAACAGCTTATGTTCATGATAATACTATCAAACCTCTTAGAGATGCTTCTTCTGCTGAATTTTCAACACCTTATGATCATGGTGCTGGCCATATTAATCCTAGAAAAGCTCTTGATCCAGGTTTAGTATATGATATCGAACCTCAGGATTACTTTGAGTTTCTTTGCTCAAAGAAACTAAGCCCTTCAGAATTAGTAGTTTTTTCTAAAAATTCGAACAGAAATTGCAAACACACTCTTGCTAGTGCTAGTGACTTGAACTATCCTGCTATATCAGTTGTGTTTCCAGCAAAACCAACCAATTTTGCTTCGACGATTCACCGCACTGTTACCAATGTTGGTCCTGCTGTTTCCAAATATCATGTCATTGTTACACCATTTAAAGGCGCTGTTGTTAAAGTTGAGCCAGATACATTGAATTTTACAAGAAAATATCAGAAACTATCTTACAAGATCAGTTTCAAAGTGACATCTAGGCAAAGTGAACCAGAATTTGGAGGTTTGGTGTGGAAAGATAGGGTGCATAAAGTAAGAAGTCCGATTGTGATAACATATATGCCACCAATGTGA
Protein sequence

>MS.gene070990.t1

MGSNSSSPDPRSLCLEGTLDRRTVTGKIVICDRGISPRVQKGQVVKNAGGVGMILTNTAANGEELVADCHLLPAVAVGEKEGKDIKQYVLTTKKATATLAFHNTRLGIRPSPIVAAFSSRGPSLLTLEILKPDIVAPGVNILAAWSGLTGPSSLPIDHRRVKFNILSGTSMSCPHVSGIAAMIKAKHPEWSPAAIKSAIMTTAYVHDNTIKPLRDASSAEFSTPYDHGAGHINPRKALDPGLVYDIEPQDYFEFLCSKKLSPSELVVFSKNSNRNCKHTLASASDLNYPAISVVFPAKPTNFASTIHRTVTNVGPAVSKYHVIVTPFKGAVVKVEPDTLNFTRKYQKLSYKISFKVTSRQSEPEFGGLVWKDRVHKVRSPIVITYMPPM