Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071068.t1 | KEH40705.1 | 96.4 | 140 | 5 | 0 | 1 | 140 | 1 | 140 | 1.20E-56 | 229.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071068.t1 | Q9FNN6 | 68.5 | 130 | 37 | 2 | 1 | 130 | 1 | 126 | 6.9e-29 | 128.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071068.t1 | A0A072VRE4 | 96.4 | 140 | 5 | 0 | 1 | 140 | 1 | 140 | 8.6e-57 | 229.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene071068.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061012 | MS.gene071068 | 0.987198 | 2.12E-169 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071068.t1 | MTR_1g033600 | 96.429 | 140 | 5 | 0 | 1 | 140 | 1 | 140 | 2.05e-99 | 281 |
| MS.gene071068.t1 | MTR_8g063600 | 60.156 | 128 | 37 | 1 | 3 | 130 | 1 | 114 | 3.57e-44 | 146 |
| MS.gene071068.t1 | MTR_8g063870 | 50.370 | 135 | 38 | 4 | 3 | 137 | 1 | 106 | 1.75e-30 | 110 |
| MS.gene071068.t1 | MTR_3g462790 | 37.879 | 132 | 75 | 2 | 4 | 135 | 35 | 159 | 7.73e-28 | 105 |
| MS.gene071068.t1 | MTR_4g111975 | 45.833 | 144 | 57 | 6 | 12 | 135 | 8 | 150 | 1.65e-27 | 103 |
| MS.gene071068.t1 | MTR_7g067080 | 40.441 | 136 | 60 | 2 | 7 | 134 | 30 | 152 | 1.30e-26 | 101 |
| MS.gene071068.t1 | MTR_1g022290 | 51.807 | 83 | 40 | 0 | 6 | 88 | 1 | 83 | 1.84e-25 | 92.8 |
| MS.gene071068.t1 | MTR_5g081860 | 44.000 | 125 | 58 | 4 | 10 | 134 | 25 | 137 | 3.89e-25 | 96.7 |
| MS.gene071068.t1 | MTR_1g111830 | 42.857 | 133 | 57 | 3 | 10 | 138 | 22 | 139 | 1.17e-24 | 95.1 |
| MS.gene071068.t1 | MTR_5g069710 | 37.879 | 132 | 64 | 3 | 9 | 135 | 26 | 144 | 7.19e-23 | 91.7 |
| MS.gene071068.t1 | MTR_3g111880 | 55.072 | 69 | 31 | 0 | 9 | 77 | 4 | 72 | 2.85e-21 | 82.0 |
| MS.gene071068.t1 | MTR_5g020170 | 50.000 | 74 | 37 | 0 | 7 | 80 | 14 | 87 | 3.03e-20 | 79.7 |
| MS.gene071068.t1 | MTR_3g116720 | 50.000 | 68 | 34 | 0 | 10 | 77 | 14 | 81 | 3.55e-20 | 79.0 |
| MS.gene071068.t1 | MTR_7g089210 | 56.250 | 64 | 28 | 0 | 15 | 78 | 15 | 78 | 8.02e-20 | 78.6 |
| MS.gene071068.t1 | MTR_6g004250 | 51.389 | 72 | 35 | 0 | 3 | 74 | 1 | 72 | 1.47e-19 | 77.4 |
| MS.gene071068.t1 | MTR_3g111920 | 41.791 | 67 | 39 | 0 | 12 | 78 | 3 | 69 | 1.38e-16 | 70.1 |
| MS.gene071068.t1 | MTR_8g077360 | 39.726 | 73 | 43 | 1 | 9 | 81 | 2 | 73 | 6.02e-16 | 68.2 |
| MS.gene071068.t1 | MTR_3g013440 | 45.205 | 73 | 39 | 1 | 3 | 74 | 1 | 73 | 8.84e-16 | 68.2 |
| MS.gene071068.t1 | MTR_8g077380 | 40.000 | 70 | 41 | 1 | 9 | 78 | 2 | 70 | 1.85e-15 | 66.6 |
| MS.gene071068.t1 | MTR_8g077390 | 40.000 | 70 | 41 | 1 | 9 | 78 | 2 | 70 | 1.85e-15 | 66.6 |
| MS.gene071068.t1 | MTR_8g077420 | 42.857 | 63 | 35 | 1 | 10 | 72 | 3 | 64 | 1.74e-13 | 61.6 |
| MS.gene071068.t1 | MTR_5g020540 | 46.032 | 63 | 33 | 1 | 10 | 72 | 2 | 63 | 3.97e-13 | 60.5 |
| MS.gene071068.t1 | MTR_1g086180 | 45.614 | 57 | 30 | 1 | 12 | 67 | 11 | 67 | 5.32e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071068.t1 | AT5G08520 | 61.314 | 137 | 48 | 5 | 1 | 137 | 1 | 132 | 2.25e-48 | 157 |
| MS.gene071068.t1 | AT2G38090 | 38.462 | 130 | 78 | 1 | 7 | 134 | 24 | 153 | 4.15e-26 | 100 |
| MS.gene071068.t1 | AT5G05790 | 42.520 | 127 | 61 | 3 | 9 | 134 | 28 | 143 | 2.01e-25 | 97.8 |
| MS.gene071068.t1 | AT5G05790 | 42.520 | 127 | 61 | 3 | 9 | 134 | 28 | 143 | 2.01e-25 | 97.8 |
| MS.gene071068.t1 | AT3G11280 | 40.323 | 124 | 56 | 2 | 12 | 134 | 33 | 139 | 1.43e-24 | 95.1 |
| MS.gene071068.t1 | AT3G11280 | 40.323 | 124 | 56 | 2 | 12 | 134 | 33 | 139 | 1.43e-24 | 95.1 |
| MS.gene071068.t1 | AT5G58900 | 40.625 | 128 | 68 | 4 | 10 | 135 | 32 | 153 | 5.13e-24 | 94.4 |
| MS.gene071068.t1 | AT4G39250 | 53.247 | 77 | 36 | 0 | 12 | 88 | 14 | 90 | 5.21e-24 | 89.4 |
| MS.gene071068.t1 | AT5G23650 | 41.406 | 128 | 72 | 2 | 5 | 132 | 6 | 130 | 1.43e-23 | 94.0 |
| MS.gene071068.t1 | AT1G49010 | 39.161 | 143 | 68 | 4 | 10 | 135 | 6 | 146 | 6.44e-22 | 89.0 |
| MS.gene071068.t1 | AT1G75250 | 50.000 | 74 | 37 | 0 | 10 | 83 | 10 | 83 | 6.81e-22 | 84.0 |
| MS.gene071068.t1 | AT1G75250 | 50.000 | 74 | 37 | 0 | 10 | 83 | 10 | 83 | 1.65e-21 | 84.0 |
| MS.gene071068.t1 | AT2G21650 | 51.429 | 70 | 34 | 0 | 12 | 81 | 14 | 83 | 3.08e-19 | 77.4 |
| MS.gene071068.t1 | AT1G19510 | 45.946 | 74 | 40 | 0 | 15 | 88 | 15 | 88 | 4.15e-19 | 77.0 |
| MS.gene071068.t1 | AT2G18328 | 52.381 | 63 | 30 | 0 | 16 | 78 | 15 | 77 | 3.25e-18 | 73.9 |
| MS.gene071068.t1 | AT5G01200 | 34.307 | 137 | 72 | 4 | 10 | 134 | 28 | 158 | 5.89e-18 | 77.8 |
| MS.gene071068.t1 | AT5G04760 | 33.858 | 127 | 66 | 4 | 9 | 135 | 3 | 111 | 9.89e-16 | 70.9 |
Find 0 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCATCGTATAATTCTTCTT+CGG | + | 11124:9821-9840 | MS.gene071068:CDS | 30.0% | |
| GAAGAATTATACGATGGTAA+AGG | - | 11124:9819-9838 | MS.gene071068:intergenic | 30.0% | |
| ATCGTATAATTCTTCTTCGG+AGG | + | 11124:9824-9843 | MS.gene071068:CDS | 35.0% | |
| TCCGAAGAAGAATTATACGA+TGG | - | 11124:9825-9844 | MS.gene071068:intergenic | 35.0% | |
| TCGTATAATTCTTCTTCGGA+GGG | + | 11124:9825-9844 | MS.gene071068:CDS | 35.0% | |
| ! | TAAGGCATTTGAAAATGCCT+TGG | + | 11124:9659-9678 | MS.gene071068:CDS | 35.0% |
| AAAAACGACGAGTCTAACCA+TGG | + | 11124:9909-9928 | MS.gene071068:CDS | 40.0% | |
| GACTTATCCTGAGGATGATT+CGG | + | 11124:9683-9702 | MS.gene071068:CDS | 40.0% | |
| GAGTCTAACCATGGAACTAA+GGG | + | 11124:9918-9937 | MS.gene071068:CDS | 40.0% | |
| GATATTAGCCAGATTGAAGC+TGG | + | 11124:9786-9805 | MS.gene071068:CDS | 40.0% | |
| TAAGCAGCATTATGAGCTCT+TGG | + | 11124:9758-9777 | MS.gene071068:CDS | 40.0% | |
| ACAAGTCATGCTAGCGATGA+AGG | + | 11124:9852-9871 | MS.gene071068:CDS | 45.0% | |
| ATCGGATCAAGAACGACGAA+AGG | + | 11124:9947-9966 | MS.gene071068:CDS | 45.0% | |
| ATCTTGAGCCCTTAGTTCCA+TGG | - | 11124:9929-9948 | MS.gene071068:intergenic | 45.0% | |
| ATGCTAGCGATGAAGGAGTT+GGG | + | 11124:9859-9878 | MS.gene071068:CDS | 45.0% | |
| ATTTCTTCCGTAGTCTTCCC+CGG | - | 11124:9738-9757 | MS.gene071068:intergenic | 45.0% | |
| CGAGTCTAACCATGGAACTA+AGG | + | 11124:9917-9936 | MS.gene071068:CDS | 45.0% | |
| TCGGATCAAGAACGACGAAA+GGG | + | 11124:9948-9967 | MS.gene071068:CDS | 45.0% | |
| TGGAACTAAGGGCTCAAGAT+CGG | + | 11124:9929-9948 | MS.gene071068:CDS | 45.0% | |
| ! | TGAGGATGATTCGGATCGAT+GGG | + | 11124:9692-9711 | MS.gene071068:CDS | 45.0% |
| !! | AAAGGGTATTGCTTGGACTG+AGG | + | 11124:9965-9984 | MS.gene071068:CDS | 45.0% |
| !! | AACGACGAAAGGGTATTGCT+TGG | + | 11124:9958-9977 | MS.gene071068:CDS | 45.0% |
| !! | AAGTGGACAATAGCTCTGAG+TGG | + | 11124:9622-9641 | MS.gene071068:CDS | 45.0% |
| !!! | GGTTAGACTCGTCGTTTTTG+TGG | - | 11124:9908-9927 | MS.gene071068:intergenic | 45.0% |
| ATCCTCAGGATAAGTCGCCA+AGG | - | 11124:9679-9698 | MS.gene071068:intergenic | 50.0% | |
| CATCGATCCGAATCATCCTC+AGG | - | 11124:9693-9712 | MS.gene071068:intergenic | 50.0% | |
| CATGCTAGCGATGAAGGAGT+TGG | + | 11124:9858-9877 | MS.gene071068:CDS | 50.0% | |
| CTTGGACTGAGGAAGAACAC+AGG | + | 11124:9976-9995 | MS.gene071068:CDS | 50.0% | |
| GCACACAACCAGCTTCAATC+TGG | - | 11124:9797-9816 | MS.gene071068:intergenic | 50.0% | |
| GGATCGATGGGAGAAGATTG+CGG | + | 11124:9704-9723 | MS.gene071068:CDS | 50.0% | |
| TGCTAGCGATGAAGGAGTTG+GGG | + | 11124:9860-9879 | MS.gene071068:CDS | 50.0% | |
| ! | CTGAGGATGATTCGGATCGA+TGG | + | 11124:9691-9710 | MS.gene071068:CDS | 50.0% |
| !! | ACAATAGCTCTGAGTGGAGC+TGG | + | 11124:9628-9647 | MS.gene071068:CDS | 50.0% |
| !! | CAATAGCTCTGAGTGGAGCT+GGG | + | 11124:9629-9648 | MS.gene071068:CDS | 50.0% |
| AAGATTGCGGCCGATGTACC+GGG | + | 11124:9717-9736 | MS.gene071068:CDS | 55.0% | |
| CGATGTACCGGGGAAGACTA+CGG | + | 11124:9728-9747 | MS.gene071068:CDS | 55.0% | |
| CGTAGTCTTCCCCGGTACAT+CGG | - | 11124:9730-9749 | MS.gene071068:intergenic | 55.0% | |
| CTAGCGATGAAGGAGTTGGG+GGG | + | 11124:9862-9881 | MS.gene071068:CDS | 55.0% | |
| GAAGATTGCGGCCGATGTAC+CGG | + | 11124:9716-9735 | MS.gene071068:CDS | 55.0% | |
| GAAGGAGTTGGGGGGAAGAA+GGG | + | 11124:9870-9889 | MS.gene071068:CDS | 55.0% | |
| GCTAGCGATGAAGGAGTTGG+GGG | + | 11124:9861-9880 | MS.gene071068:CDS | 55.0% | |
| GGGAAGAAGGGTGGTCACAA+TGG | + | 11124:9882-9901 | MS.gene071068:CDS | 55.0% | |
| GTGGAGCTGGGAGCAAGATA+AGG | + | 11124:9641-9660 | MS.gene071068:CDS | 55.0% | |
| TGAAGGAGTTGGGGGGAAGA+AGG | + | 11124:9869-9888 | MS.gene071068:CDS | 55.0% | |
| TGCCTTGGCGACTTATCCTG+AGG | + | 11124:9674-9693 | MS.gene071068:CDS | 55.0% | |
| AGATTGCGGCCGATGTACCG+GGG | + | 11124:9718-9737 | MS.gene071068:CDS | 60.0% | |
| GGAGTTGGGGGGAAGAAGGG+TGG | + | 11124:9873-9892 | MS.gene071068:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11124 | gene | 9609 | 10031 | 9609 | ID=MS.gene071068 |
| 11124 | mRNA | 9609 | 10031 | 9609 | ID=MS.gene071068.t1;Parent=MS.gene071068 |
| 11124 | exon | 9609 | 10031 | 9609 | ID=MS.gene071068.t1.exon1;Parent=MS.gene071068.t1 |
| 11124 | CDS | 9609 | 10031 | 9609 | ID=cds.MS.gene071068.t1;Parent=MS.gene071068.t1 |
| Gene Sequence |
| Protein sequence |
