Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07118.t1 | XP_003593610.1 | 86.8 | 234 | 12 | 1 | 1 | 215 | 1 | 234 | 1.60E-105 | 392.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07118.t1 | Q9ZQH0 | 64.9 | 225 | 60 | 1 | 1 | 206 | 1 | 225 | 3.6e-77 | 289.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07118.t1 | G7ISC8 | 86.8 | 234 | 12 | 1 | 1 | 215 | 1 | 234 | 1.2e-105 | 392.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050844 | MS.gene07118 | 0.810063 | 1.39E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene07118 | 0.824316 | 8.62E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07118.t1 | MTR_2g014120 | 86.752 | 234 | 12 | 1 | 1 | 215 | 1 | 234 | 6.35e-142 | 395 |
| MS.gene07118.t1 | MTR_7g108540 | 86.752 | 234 | 12 | 1 | 1 | 215 | 1 | 234 | 9.02e-142 | 395 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07118.t1 | AT2G27490 | 64.889 | 225 | 60 | 1 | 1 | 206 | 1 | 225 | 7.03e-100 | 289 |
| MS.gene07118.t1 | AT2G27490 | 64.889 | 225 | 60 | 1 | 1 | 206 | 1 | 225 | 7.03e-100 | 289 |
| MS.gene07118.t1 | AT2G27490 | 64.889 | 225 | 60 | 1 | 1 | 206 | 1 | 225 | 7.03e-100 | 289 |
| MS.gene07118.t1 | AT2G27490 | 64.889 | 225 | 60 | 1 | 1 | 206 | 1 | 225 | 7.03e-100 | 289 |
Find 49 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCTCTGAATCTGTGTTTC+AGG | 0.221958 | 7.1:+5186346 | None:intergenic |
| ATCAACCCACACAACAATTA+TGG | 0.226242 | 7.1:+5186369 | None:intergenic |
| CCTTGACATGGTATGAGTTT+TGG | 0.281857 | 7.1:-5186159 | MS.gene07118:CDS |
| TTGAGAAATTGACGCTTATC+AGG | 0.303211 | 7.1:+5186607 | None:intergenic |
| TGAACAATTTCAGAACGTTT+TGG | 0.336190 | 7.1:-5186197 | MS.gene07118:CDS |
| AGATGCCGCTGGATGGTAAA+AGG | 0.351308 | 7.1:-5186270 | MS.gene07118:CDS |
| TGACGTCCCTTTGTTATTTG+AGG | 0.363223 | 7.1:-5186416 | MS.gene07118:CDS |
| ATAGAATAAATGAGGATTGT+TGG | 0.388584 | 7.1:-5186945 | None:intergenic |
| ACTACATCAGCGTCGACAAC+AGG | 0.406465 | 7.1:+5186858 | None:intergenic |
| GTCGTGAGACTTGAATAAAT+TGG | 0.409640 | 7.1:+5186884 | None:intergenic |
| TCAACCCACACAACAATTAT+GGG | 0.455800 | 7.1:+5186370 | None:intergenic |
| TACTAGGATGCGTTGAAGAA+AGG | 0.463219 | 7.1:-5186733 | MS.gene07118:intron |
| ACTAGGATGCGTTGAAGAAA+GGG | 0.487032 | 7.1:-5186732 | MS.gene07118:intron |
| AACACAGATTCAGAGACTAT+TGG | 0.491505 | 7.1:-5186341 | MS.gene07118:CDS |
| ATTCTCGCTTCATTTACCTC+AGG | 0.499986 | 7.1:-5186112 | MS.gene07118:CDS |
| CTCATACCATGTCAAGGGTC+CGG | 0.506695 | 7.1:+5186165 | None:intergenic |
| ATGAGGATTGTTGGACTGAC+TGG | 0.512619 | 7.1:-5186936 | MS.gene07118:CDS |
| CGAAGCCCATAATTGTTGTG+TGG | 0.541419 | 7.1:-5186375 | MS.gene07118:CDS |
| TGAAGAAAGGGAGCGGCGGA+TGG | 0.543910 | 7.1:-5186720 | MS.gene07118:CDS |
| TCTTAGCCTCAAATAACAAA+GGG | 0.545675 | 7.1:+5186410 | None:intergenic |
| AGACTTGAATAAATTGGAAA+CGG | 0.547925 | 7.1:+5186890 | None:intergenic |
| AAAACGATTTGGCCAAGTCT+GGG | 0.565501 | 7.1:+5186637 | None:intergenic |
| GGTCAATGCTCAGATGCCGC+TGG | 0.566232 | 7.1:-5186281 | MS.gene07118:CDS |
| TTTGGGAAGTTGTGAAGCTG+TGG | 0.578755 | 7.1:-5186465 | MS.gene07118:intron |
| CGAGGAGGATGGTCGGAACA+AGG | 0.580768 | 7.1:-5186302 | MS.gene07118:CDS |
| GTTGTGAAGCTGTGGTTGAA+AGG | 0.585087 | 7.1:-5186457 | MS.gene07118:CDS |
| ATCTTAGCCTCAAATAACAA+AGG | 0.586193 | 7.1:+5186409 | None:intergenic |
| CCAAAACTCATACCATGTCA+AGG | 0.598156 | 7.1:+5186159 | None:intergenic |
| AGTCTAGCGAGGAGGATGGT+CGG | 0.598811 | 7.1:-5186309 | MS.gene07118:CDS |
| AGTTGTTGCCGCGTTTGGGG+AGG | 0.599566 | 7.1:-5186692 | MS.gene07118:CDS |
| GAAGTTAACAGACCCAGACT+TGG | 0.601753 | 7.1:-5186649 | MS.gene07118:CDS |
| AAAAGTTGTTGCCGCGTTTG+GGG | 0.604856 | 7.1:-5186695 | MS.gene07118:CDS |
| GGGTCACCGGACCCTTGACA+TGG | 0.606552 | 7.1:-5186171 | MS.gene07118:CDS |
| GAGGAAATTCTACTAGACAA+TGG | 0.608332 | 7.1:-5186673 | MS.gene07118:CDS |
| GATAAGTCTAGCGAGGAGGA+TGG | 0.608356 | 7.1:-5186313 | MS.gene07118:CDS |
| AATGCTCAGATGCCGCTGGA+TGG | 0.610763 | 7.1:-5186277 | MS.gene07118:CDS |
| GAAGCCCATAATTGTTGTGT+GGG | 0.612377 | 7.1:-5186374 | MS.gene07118:CDS |
| GCTGTGGTTGAAAGGTTACA+AGG | 0.613383 | 7.1:-5186449 | MS.gene07118:CDS |
| TAGAATTTCCTCCCCAAACG+CGG | 0.631720 | 7.1:+5186684 | None:intergenic |
| CAAAACTCATACCATGTCAA+GGG | 0.641999 | 7.1:+5186160 | None:intergenic |
| AGGATTGTTGGACTGACTGG+TGG | 0.644760 | 7.1:-5186933 | MS.gene07118:CDS |
| GATGCGTTGAAGAAAGGGAG+CGG | 0.653195 | 7.1:-5186727 | MS.gene07118:CDS |
| GGCAAGAGATAAGTCTAGCG+AGG | 0.657006 | 7.1:-5186320 | MS.gene07118:CDS |
| GATATAGTAATCGACAACAC+TGG | 0.662812 | 7.1:-5186238 | MS.gene07118:CDS |
| AAGAGATAAGTCTAGCGAGG+AGG | 0.683714 | 7.1:-5186317 | MS.gene07118:CDS |
| GCGTTGAAGAAAGGGAGCGG+CGG | 0.690363 | 7.1:-5186724 | MS.gene07118:CDS |
| CTACATCAGCGTCGACAACA+GGG | 0.699636 | 7.1:+5186859 | None:intergenic |
| CATACATAGAACAACACCTG+AGG | 0.740107 | 7.1:+5186096 | None:intergenic |
| CTGACTGGTGGAATAGCAAG+TGG | 0.743435 | 7.1:-5186921 | MS.gene07118:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTACTTTATTTTTGAAGTTT+CGG | - | chr7.1:5186467-5186486 | MS.gene07118:intron | 15.0% |
| ! | AAATTATAAGCAAAGCCAAA+AGG | + | chr7.1:5186210-5186229 | None:intergenic | 25.0% |
| ! | AGACTTGAATAAATTGGAAA+CGG | + | chr7.1:5186118-5186137 | None:intergenic | 25.0% |
| ! | GGTATTCTAAATTTATGTTG+TGG | - | chr7.1:5186423-5186442 | MS.gene07118:CDS | 25.0% |
| ! | TTATTGGGATTTGAATTACT+AGG | - | chr7.1:5186256-5186275 | MS.gene07118:CDS | 25.0% |
| !!! | TTTGGCTTTGCTTATAATTT+GGG | - | chr7.1:5186210-5186229 | MS.gene07118:CDS | 25.0% |
| !!! | TTTTGGCTTTGCTTATAATT+TGG | - | chr7.1:5186209-5186228 | MS.gene07118:CDS | 25.0% |
| ATAAGCGTCAATTTCTCAAT+CGG | - | chr7.1:5186402-5186421 | MS.gene07118:CDS | 30.0% | |
| ATCTTAGCCTCAAATAACAA+AGG | + | chr7.1:5186599-5186618 | None:intergenic | 30.0% | |
| TCTTAGCCTCAAATAACAAA+GGG | + | chr7.1:5186598-5186617 | None:intergenic | 30.0% | |
| ! | TGAACAATTTCAGAACGTTT+TGG | - | chr7.1:5186808-5186827 | MS.gene07118:intron | 30.0% |
| !! | TTGGCTTTGCTTATAATTTG+GGG | - | chr7.1:5186211-5186230 | MS.gene07118:CDS | 30.0% |
| !!! | AATTTCAGAACGTTTTGGTT+CGG | - | chr7.1:5186813-5186832 | MS.gene07118:intron | 30.0% |
| !!! | TAATGGTTGTTTTAGGTTGT+TGG | - | chr7.1:5186493-5186512 | MS.gene07118:intron | 30.0% |
| !!! | TTCGTGTTTTTTTCACCTTT+TGG | - | chr7.1:5186192-5186211 | MS.gene07118:CDS | 30.0% |
| !!! | TTTTGAAGTTTCGGTTGTAA+TGG | - | chr7.1:5186476-5186495 | MS.gene07118:intron | 30.0% |
| AACACAGATTCAGAGACTAT+TGG | - | chr7.1:5186664-5186683 | MS.gene07118:CDS | 35.0% | |
| ATCAACCCACACAACAATTA+TGG | + | chr7.1:5186639-5186658 | None:intergenic | 35.0% | |
| CAAAACTCATACCATGTCAA+GGG | + | chr7.1:5186848-5186867 | None:intergenic | 35.0% | |
| GAGGAAATTCTACTAGACAA+TGG | - | chr7.1:5186332-5186351 | MS.gene07118:CDS | 35.0% | |
| GATATAGTAATCGACAACAC+TGG | - | chr7.1:5186767-5186786 | MS.gene07118:intron | 35.0% | |
| GTCGTGAGACTTGAATAAAT+TGG | + | chr7.1:5186124-5186143 | None:intergenic | 35.0% | |
| TCAACCCACACAACAATTAT+GGG | + | chr7.1:5186638-5186657 | None:intergenic | 35.0% | |
| TTGAGAAATTGACGCTTATC+AGG | + | chr7.1:5186401-5186420 | None:intergenic | 35.0% | |
| !! | TCGGTTGTAATGGTTGTTTT+AGG | - | chr7.1:5186486-5186505 | MS.gene07118:intron | 35.0% |
| !!! | ATTTCAGAACGTTTTGGTTC+GGG | - | chr7.1:5186814-5186833 | MS.gene07118:intron | 35.0% |
| AAAAAAGTTGTTGCCGCGTT+TGG | - | chr7.1:5186308-5186327 | MS.gene07118:CDS | 40.0% | |
| AAAAAGTTGTTGCCGCGTTT+GGG | - | chr7.1:5186309-5186328 | MS.gene07118:CDS | 40.0% | |
| AAGATCCCCGAGGATATATA+TGG | + | chr7.1:5186521-5186540 | None:intergenic | 40.0% | |
| ACTAGGATGCGTTGAAGAAA+GGG | - | chr7.1:5186273-5186292 | MS.gene07118:CDS | 40.0% | |
| AGTCTCTGAATCTGTGTTTC+AGG | + | chr7.1:5186662-5186681 | None:intergenic | 40.0% | |
| ATTCTCGCTTCATTTACCTC+AGG | - | chr7.1:5186893-5186912 | MS.gene07118:CDS | 40.0% | |
| CAGGATCAGCAAAAACGATT+TGG | + | chr7.1:5186382-5186401 | None:intergenic | 40.0% | |
| CATACATAGAACAACACCTG+AGG | + | chr7.1:5186912-5186931 | None:intergenic | 40.0% | |
| CCAAAACTCATACCATGTCA+AGG | + | chr7.1:5186849-5186868 | None:intergenic | 40.0% | |
| GAAGCCCATAATTGTTGTGT+GGG | - | chr7.1:5186631-5186650 | MS.gene07118:CDS | 40.0% | |
| TACTAGGATGCGTTGAAGAA+AGG | - | chr7.1:5186272-5186291 | MS.gene07118:CDS | 40.0% | |
| TTGGCTCCATATATATCCTC+GGG | - | chr7.1:5186512-5186531 | MS.gene07118:intron | 40.0% | |
| ! | AAAAACGATTTGGCCAAGTC+TGG | + | chr7.1:5186372-5186391 | None:intergenic | 40.0% |
| ! | AAAACGATTTGGCCAAGTCT+GGG | + | chr7.1:5186371-5186390 | None:intergenic | 40.0% |
| ! | ATATATCCTCGGGGATCTTT+TGG | - | chr7.1:5186522-5186541 | MS.gene07118:intron | 40.0% |
| ! | CCTTGACATGGTATGAGTTT+TGG | - | chr7.1:5186846-5186865 | MS.gene07118:intron | 40.0% |
| ! | GTTGGCTCCATATATATCCT+CGG | - | chr7.1:5186511-5186530 | MS.gene07118:intron | 40.0% |
| ! | TATATCCTCGGGGATCTTTT+GGG | - | chr7.1:5186523-5186542 | MS.gene07118:intron | 40.0% |
| ! | TTACTCCTTTTACCATCCAG+CGG | + | chr7.1:5186743-5186762 | None:intergenic | 40.0% |
| !! | TGACGTCCCTTTGTTATTTG+AGG | - | chr7.1:5186589-5186608 | MS.gene07118:intron | 40.0% |
| AAAAGTTGTTGCCGCGTTTG+GGG | - | chr7.1:5186310-5186329 | MS.gene07118:CDS | 45.0% | |
| AAGAGATAAGTCTAGCGAGG+AGG | - | chr7.1:5186688-5186707 | MS.gene07118:CDS | 45.0% | |
| AATAACCACCGACCATGAGA+GGG | + | chr7.1:5186241-5186260 | None:intergenic | 45.0% | |
| ATGAGGATTGTTGGACTGAC+TGG | - | chr7.1:5186069-5186088 | MS.gene07118:CDS | 45.0% | |
| ATTTGGGGAAAACCCTCTCA+TGG | - | chr7.1:5186226-5186245 | MS.gene07118:CDS | 45.0% | |
| CGAAGCCCATAATTGTTGTG+TGG | - | chr7.1:5186630-5186649 | MS.gene07118:CDS | 45.0% | |
| GAAGTTAACAGACCCAGACT+TGG | - | chr7.1:5186356-5186375 | MS.gene07118:CDS | 45.0% | |
| GCTGTGGTTGAAAGGTTACA+AGG | - | chr7.1:5186556-5186575 | MS.gene07118:intron | 45.0% | |
| GTTGTGAAGCTGTGGTTGAA+AGG | - | chr7.1:5186548-5186567 | MS.gene07118:intron | 45.0% | |
| TAGAATTTCCTCCCCAAACG+CGG | + | chr7.1:5186324-5186343 | None:intergenic | 45.0% | |
| TCTCATGGTCGGTGGTTATT+GGG | - | chr7.1:5186241-5186260 | MS.gene07118:CDS | 45.0% | |
| TGGCTCCATATATATCCTCG+GGG | - | chr7.1:5186513-5186532 | MS.gene07118:intron | 45.0% | |
| TTTGGGAAGTTGTGAAGCTG+TGG | - | chr7.1:5186540-5186559 | MS.gene07118:intron | 45.0% | |
| !! | GGTATGAGTTTTGGCGTTCT+AGG | - | chr7.1:5186855-5186874 | MS.gene07118:CDS | 45.0% |
| AACTTCCCAAAAGATCCCCG+AGG | + | chr7.1:5186531-5186550 | None:intergenic | 50.0% | |
| AGATGCCGCTGGATGGTAAA+AGG | - | chr7.1:5186735-5186754 | MS.gene07118:intron | 50.0% | |
| AGGATTGTTGGACTGACTGG+TGG | - | chr7.1:5186072-5186091 | MS.gene07118:CDS | 50.0% | |
| CAATAACCACCGACCATGAG+AGG | + | chr7.1:5186242-5186261 | None:intergenic | 50.0% | |
| CTCATACCATGTCAAGGGTC+CGG | + | chr7.1:5186843-5186862 | None:intergenic | 50.0% | |
| CTCTCATGGTCGGTGGTTAT+TGG | - | chr7.1:5186240-5186259 | MS.gene07118:CDS | 50.0% | |
| CTGACTGGTGGAATAGCAAG+TGG | - | chr7.1:5186084-5186103 | MS.gene07118:CDS | 50.0% | |
| GATAAGTCTAGCGAGGAGGA+TGG | - | chr7.1:5186692-5186711 | MS.gene07118:CDS | 50.0% | |
| GATGCGTTGAAGAAAGGGAG+CGG | - | chr7.1:5186278-5186297 | MS.gene07118:CDS | 50.0% | |
| GGCAAGAGATAAGTCTAGCG+AGG | - | chr7.1:5186685-5186704 | MS.gene07118:CDS | 50.0% | |
| !! | ACTACATCAGCGTCGACAAC+AGG | + | chr7.1:5186150-5186169 | None:intergenic | 50.0% |
| !! | CTACATCAGCGTCGACAACA+GGG | + | chr7.1:5186149-5186168 | None:intergenic | 50.0% |
| !! | GAGTTTTGGCGTTCTAGGCA+GGG | - | chr7.1:5186860-5186879 | MS.gene07118:CDS | 50.0% |
| !! | TGAGTTTTGGCGTTCTAGGC+AGG | - | chr7.1:5186859-5186878 | MS.gene07118:CDS | 50.0% |
| !!! | AACGTTTTGGTTCGGGTCAC+CGG | - | chr7.1:5186821-5186840 | MS.gene07118:intron | 50.0% |
| AATGCTCAGATGCCGCTGGA+TGG | - | chr7.1:5186728-5186747 | MS.gene07118:CDS | 55.0% | |
| AGTCTAGCGAGGAGGATGGT+CGG | - | chr7.1:5186696-5186715 | MS.gene07118:CDS | 55.0% | |
| GAAAACCCTCTCATGGTCGG+TGG | - | chr7.1:5186233-5186252 | MS.gene07118:CDS | 55.0% | |
| GGGGAAAACCCTCTCATGGT+CGG | - | chr7.1:5186230-5186249 | MS.gene07118:CDS | 55.0% | |
| AGTTGTTGCCGCGTTTGGGG+AGG | - | chr7.1:5186313-5186332 | MS.gene07118:CDS | 60.0% | |
| CGAGGAGGATGGTCGGAACA+AGG | - | chr7.1:5186703-5186722 | MS.gene07118:CDS | 60.0% | |
| GCGTTGAAGAAAGGGAGCGG+CGG | - | chr7.1:5186281-5186300 | MS.gene07118:CDS | 60.0% | |
| GGTCAATGCTCAGATGCCGC+TGG | - | chr7.1:5186724-5186743 | MS.gene07118:CDS | 60.0% | |
| TGAAGAAAGGGAGCGGCGGA+TGG | - | chr7.1:5186285-5186304 | MS.gene07118:CDS | 60.0% | |
| GGGTCACCGGACCCTTGACA+TGG | - | chr7.1:5186834-5186853 | MS.gene07118:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 5186069 | 5186958 | 5186069 | ID=MS.gene07118 |
| chr7.1 | mRNA | 5186069 | 5186958 | 5186069 | ID=MS.gene07118.t1;Parent=MS.gene07118 |
| chr7.1 | exon | 5186851 | 5186958 | 5186851 | ID=MS.gene07118.t1.exon1;Parent=MS.gene07118.t1 |
| chr7.1 | CDS | 5186851 | 5186958 | 5186851 | ID=cds.MS.gene07118.t1;Parent=MS.gene07118.t1 |
| chr7.1 | exon | 5186620 | 5186749 | 5186620 | ID=MS.gene07118.t1.exon2;Parent=MS.gene07118.t1 |
| chr7.1 | CDS | 5186620 | 5186749 | 5186620 | ID=cds.MS.gene07118.t1;Parent=MS.gene07118.t1 |
| chr7.1 | exon | 5186069 | 5186478 | 5186069 | ID=MS.gene07118.t1.exon3;Parent=MS.gene07118.t1 |
| chr7.1 | CDS | 5186069 | 5186478 | 5186069 | ID=cds.MS.gene07118.t1;Parent=MS.gene07118.t1 |
| Gene Sequence |
| Protein sequence |
