Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071253.t1 | XP_013445572.1 | 99.1 | 106 | 1 | 0 | 1 | 106 | 4 | 109 | 6.10E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071253.t1 | Q39580 | 57.6 | 85 | 34 | 1 | 2 | 86 | 7 | 89 | 7.6e-20 | 97.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071253.t1 | A0A072TRW8 | 99.1 | 106 | 1 | 0 | 1 | 106 | 4 | 109 | 4.4e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059894 | MS.gene071253 | 0.80832 | 3.28E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTAATAAATTAAAACTAAG+AGG | - | 54861:2257-2276 | MS.gene071253:CDS | 20.0% |
| ! | AAACTAAGAGGATTGTTTAT+TGG | - | 54861:2269-2288 | MS.gene071253:CDS | 25.0% |
| ! | AATTATTGCAGGAATTTGAT+AGG | - | 54861:2340-2359 | MS.gene071253:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | + | 54861:2205-2224 | MS.gene071253:intergenic | 25.0% |
| ! | ATGTGTTAGTTAATTATTGC+AGG | - | 54861:2329-2348 | MS.gene071253:intron | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | - | 54861:2341-2360 | MS.gene071253:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | + | 54861:2232-2251 | MS.gene071253:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | - | 54861:2236-2255 | MS.gene071253:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | - | 54861:2432-2451 | MS.gene071253:CDS | 25.0% |
| AGATTTCTATGATGTAACTG+AGG | - | 54861:2206-2225 | MS.gene071253:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | + | 54861:2206-2225 | MS.gene071253:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | - | 54861:2459-2478 | MS.gene071253:CDS | 30.0% |
| !!! | CTATTTTTGTATAGGTAGCA+TGG | - | 54861:2440-2459 | MS.gene071253:CDS | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | - | 54861:2506-2525 | MS.gene071253:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | + | 54861:2199-2218 | MS.gene071253:intergenic | 35.0% |
| ! | CATGGCAATTTTGCTCTTTA+AGG | - | 54861:2458-2477 | MS.gene071253:CDS | 35.0% |
| !! | AACTGATTTTCTTCTGCTTG+TGG | + | 54861:2495-2514 | MS.gene071253:intergenic | 35.0% |
| CAGGAATTTGATAGGGTTCA+TGG | - | 54861:2348-2367 | MS.gene071253:intron | 40.0% | |
| TCCTTTGTGACACATTGTAG+TGG | - | 54861:2408-2427 | MS.gene071253:intron | 40.0% | |
| !!! | TGCATTGTAGGCACTGATTT+TGG | - | 54861:2384-2403 | MS.gene071253:intron | 40.0% |
| GCCACTACAATGTGTCACAA+AGG | + | 54861:2412-2431 | MS.gene071253:intergenic | 45.0% | |
| TTTGATAGGGTTCATGGACC+TGG | - | 54861:2354-2373 | MS.gene071253:intron | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | - | 54861:2468-2487 | MS.gene071253:CDS | 45.0% |
| !!! | TTTTAATGTAATTTTTTTTA+TGG | - | 54861:2295-2314 | MS.gene071253:CDS | 5.0% |
| ATAGGGTTCATGGACCTGGT+TGG | - | 54861:2358-2377 | MS.gene071253:intron | 50.0% | |
| CCTACAATGCATTGCCAACC+AGG | + | 54861:2375-2394 | MS.gene071253:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | - | 54861:2372-2391 | MS.gene071253:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 54861 | gene | 2127 | 2542 | 2127 | ID=MS.gene071253 |
| 54861 | mRNA | 2127 | 2542 | 2127 | ID=MS.gene071253.t1;Parent=MS.gene071253 |
| 54861 | exon | 2412 | 2542 | 2412 | ID=MS.gene071253.t1.exon1;Parent=MS.gene071253.t1 |
| 54861 | CDS | 2412 | 2542 | 2412 | ID=cds.MS.gene071253.t1;Parent=MS.gene071253.t1 |
| 54861 | exon | 2127 | 2318 | 2127 | ID=MS.gene071253.t1.exon2;Parent=MS.gene071253.t1 |
| 54861 | CDS | 2127 | 2318 | 2127 | ID=cds.MS.gene071253.t1;Parent=MS.gene071253.t1 |
| Gene Sequence |
| Protein sequence |
