Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071254.t1 | XP_013445572.1 | 99.1 | 106 | 1 | 0 | 1 | 106 | 4 | 109 | 6.10E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071254.t1 | Q39580 | 57.6 | 85 | 34 | 1 | 2 | 86 | 7 | 89 | 7.6e-20 | 97.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071254.t1 | A0A072TRW8 | 99.1 | 106 | 1 | 0 | 1 | 106 | 4 | 109 | 4.4e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052077 | MS.gene071254 | 0.812639 | 3.83E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene071254 | -0.801953 | 7.06E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene071254 | 0.814979 | 1.17E-51 | -1.69E-46 |
| MS.gene059894 | MS.gene071254 | 0.812569 | 3.97E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTAGGCACTGATTT+TGG | 0.221609 | 8.1:-48921495 | MS.gene071254:CDS |
| CAGGAATTTGATAGGGTTCA+TGG | 0.298316 | 8.1:-48921531 | MS.gene071254:CDS |
| CAGTTACATCATAGAAATCT+AGG | 0.300776 | 8.1:+48921676 | None:intergenic |
| AATTATTGCAGGAATTTGAT+AGG | 0.304941 | 8.1:-48921539 | MS.gene071254:intron |
| ATCATAGAAATCTAGGGCTT+TGG | 0.353606 | 8.1:+48921683 | None:intergenic |
| TTGCTCTTTAAGGGATCTGC+TGG | 0.420352 | 8.1:-48921411 | MS.gene071254:CDS |
| CCTGGTTGGCAATGCATTGT+AGG | 0.440795 | 8.1:-48921507 | MS.gene071254:CDS |
| CTAGAAGGCAAGGCAGTGAT+TGG | 0.481296 | 8.1:-48921756 | None:intergenic |
| CCTACAATGCATTGCCAACC+AGG | 0.504627 | 8.1:+48921507 | None:intergenic |
| AAATCAGTTCTCAGCAATAG+AGG | 0.512873 | 8.1:-48921373 | MS.gene071254:CDS |
| TATAGCTCGATTTATAAAGA+AGG | 0.514762 | 8.1:-48921643 | MS.gene071254:intron |
| TTTGATAGGGTTCATGGACC+TGG | 0.522418 | 8.1:-48921525 | MS.gene071254:CDS |
| ATTATTGCAGGAATTTGATA+GGG | 0.524356 | 8.1:-48921538 | MS.gene071254:intron |
| TATAAATCGAGCTATATCAA+TGG | 0.561045 | 8.1:+48921650 | None:intergenic |
| ATTCATCATGCTAGAAGGCA+AGG | 0.581622 | 8.1:-48921766 | None:intergenic |
| TCCTTTGTGACACATTGTAG+TGG | 0.583602 | 8.1:-48921471 | MS.gene071254:CDS |
| AGTTACATCATAGAAATCTA+GGG | 0.585718 | 8.1:+48921677 | None:intergenic |
| ATAGGGTTCATGGACCTGGT+TGG | 0.594187 | 8.1:-48921521 | MS.gene071254:CDS |
| GCCACTACAATGTGTCACAA+AGG | 0.659746 | 8.1:+48921470 | None:intergenic |
| AGATTTCTATGATGTAACTG+AGG | 0.680263 | 8.1:-48921673 | MS.gene071254:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTAATAAATTAAAACTAAG+AGG | - | chr8.1:48921489-48921508 | MS.gene071254:CDS | 20.0% |
| ! | AAACTAAGAGGATTGTTTAT+TGG | - | chr8.1:48921501-48921520 | MS.gene071254:CDS | 25.0% |
| ! | AATTATTGCAGGAATTTGAT+AGG | - | chr8.1:48921572-48921591 | MS.gene071254:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | + | chr8.1:48921437-48921456 | None:intergenic | 25.0% |
| ! | ATGTGTTAGTTAATTATTGC+AGG | - | chr8.1:48921561-48921580 | MS.gene071254:intron | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | - | chr8.1:48921573-48921592 | MS.gene071254:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | + | chr8.1:48921464-48921483 | None:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | - | chr8.1:48921468-48921487 | MS.gene071254:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | - | chr8.1:48921664-48921683 | MS.gene071254:CDS | 25.0% |
| AGATTTCTATGATGTAACTG+AGG | - | chr8.1:48921438-48921457 | MS.gene071254:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | + | chr8.1:48921438-48921457 | None:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | - | chr8.1:48921691-48921710 | MS.gene071254:CDS | 30.0% |
| !!! | CTATTTTTGTATAGGTAGCA+TGG | - | chr8.1:48921672-48921691 | MS.gene071254:CDS | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | - | chr8.1:48921738-48921757 | MS.gene071254:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | + | chr8.1:48921431-48921450 | None:intergenic | 35.0% |
| ! | CATGGCAATTTTGCTCTTTA+AGG | - | chr8.1:48921690-48921709 | MS.gene071254:CDS | 35.0% |
| !! | AACTGATTTTCTTCTGCTTG+TGG | + | chr8.1:48921727-48921746 | None:intergenic | 35.0% |
| CAGGAATTTGATAGGGTTCA+TGG | - | chr8.1:48921580-48921599 | MS.gene071254:intron | 40.0% | |
| TCCTTTGTGACACATTGTAG+TGG | - | chr8.1:48921640-48921659 | MS.gene071254:intron | 40.0% | |
| !!! | TGCATTGTAGGCACTGATTT+TGG | - | chr8.1:48921616-48921635 | MS.gene071254:intron | 40.0% |
| GCCACTACAATGTGTCACAA+AGG | + | chr8.1:48921644-48921663 | None:intergenic | 45.0% | |
| TTTGATAGGGTTCATGGACC+TGG | - | chr8.1:48921586-48921605 | MS.gene071254:intron | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | - | chr8.1:48921700-48921719 | MS.gene071254:CDS | 45.0% |
| !!! | TTTTAATGTAATTTTTTTTA+TGG | - | chr8.1:48921527-48921546 | MS.gene071254:CDS | 5.0% |
| ATAGGGTTCATGGACCTGGT+TGG | - | chr8.1:48921590-48921609 | MS.gene071254:intron | 50.0% | |
| CCTACAATGCATTGCCAACC+AGG | + | chr8.1:48921607-48921626 | None:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | - | chr8.1:48921604-48921623 | MS.gene071254:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 48921359 | 48921774 | 48921359 | ID=MS.gene071254 |
| chr8.1 | mRNA | 48921359 | 48921774 | 48921359 | ID=MS.gene071254.t1;Parent=MS.gene071254 |
| chr8.1 | exon | 48921644 | 48921774 | 48921644 | ID=MS.gene071254.t1.exon1;Parent=MS.gene071254.t1 |
| chr8.1 | CDS | 48921644 | 48921774 | 48921644 | ID=cds.MS.gene071254.t1;Parent=MS.gene071254.t1 |
| chr8.1 | exon | 48921359 | 48921550 | 48921359 | ID=MS.gene071254.t1.exon2;Parent=MS.gene071254.t1 |
| chr8.1 | CDS | 48921359 | 48921550 | 48921359 | ID=cds.MS.gene071254.t1;Parent=MS.gene071254.t1 |
| Gene Sequence |
| Protein sequence |
