Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071484.t1 | XP_003607540.2 | 98.1 | 632 | 9 | 3 | 1 | 630 | 1 | 631 | 0.00E+00 | 1172.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071484.t1 | O25225 | 46.1 | 570 | 290 | 6 | 57 | 622 | 4 | 560 | 6.8e-140 | 499.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071484.t1 | G7JUZ7 | 98.1 | 632 | 9 | 3 | 1 | 630 | 1 | 631 | 0.0e+00 | 1172.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene89744 | MS.gene071484 | PPI |
| MS.gene018869 | MS.gene071484 | PPI |
| MS.gene061606 | MS.gene071484 | PPI |
| MS.gene040952 | MS.gene071484 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071484.t1 | MTR_4g079360 | 98.101 | 632 | 9 | 3 | 1 | 630 | 1 | 631 | 0.0 | 1259 |
| MS.gene071484.t1 | MTR_1g052535 | 42.254 | 568 | 310 | 7 | 57 | 619 | 82 | 636 | 1.96e-141 | 427 |
| MS.gene071484.t1 | MTR_1g052535 | 43.627 | 408 | 214 | 5 | 57 | 459 | 82 | 478 | 2.64e-104 | 325 |
| MS.gene071484.t1 | MTR_3g097250 | 28.820 | 517 | 286 | 20 | 57 | 534 | 83 | 556 | 1.68e-35 | 142 |
| MS.gene071484.t1 | MTR_7g009740 | 31.128 | 257 | 135 | 4 | 54 | 292 | 64 | 296 | 6.52e-28 | 119 |
| MS.gene071484.t1 | MTR_4g119340 | 41.045 | 134 | 73 | 1 | 53 | 180 | 10 | 143 | 6.47e-24 | 107 |
| MS.gene071484.t1 | MTR_7g044770 | 35.870 | 184 | 107 | 3 | 8 | 180 | 11 | 194 | 9.67e-24 | 107 |
| MS.gene071484.t1 | MTR_7g094460 | 29.180 | 305 | 181 | 10 | 4 | 295 | 17 | 299 | 3.30e-21 | 97.4 |
| MS.gene071484.t1 | MTR_2g069310 | 40.816 | 147 | 65 | 4 | 55 | 180 | 17 | 162 | 1.16e-20 | 97.4 |
| MS.gene071484.t1 | MTR_2g069050 | 40.816 | 147 | 65 | 4 | 55 | 180 | 17 | 162 | 1.26e-20 | 97.1 |
| MS.gene071484.t1 | MTR_1g048000 | 40.816 | 147 | 65 | 4 | 55 | 180 | 17 | 162 | 1.26e-20 | 97.1 |
| MS.gene071484.t1 | MTR_2g021300 | 29.368 | 269 | 171 | 8 | 36 | 295 | 28 | 286 | 3.86e-20 | 94.0 |
| MS.gene071484.t1 | MTR_2g069320 | 38.776 | 147 | 68 | 3 | 55 | 180 | 17 | 162 | 4.68e-20 | 95.5 |
| MS.gene071484.t1 | MTR_4g101750 | 40.310 | 129 | 71 | 2 | 58 | 180 | 93 | 221 | 7.40e-19 | 91.3 |
| MS.gene071484.t1 | MTR_4g101750 | 40.310 | 129 | 71 | 2 | 58 | 180 | 93 | 221 | 7.54e-19 | 91.3 |
| MS.gene071484.t1 | MTR_2g020660 | 35.185 | 162 | 92 | 3 | 33 | 181 | 27 | 188 | 2.50e-18 | 89.7 |
| MS.gene071484.t1 | MTR_3g076660 | 28.906 | 256 | 162 | 7 | 59 | 300 | 94 | 343 | 1.38e-17 | 86.7 |
| MS.gene071484.t1 | MTR_4g116430 | 35.714 | 140 | 78 | 2 | 53 | 180 | 135 | 274 | 3.19e-17 | 86.7 |
| MS.gene071484.t1 | MTR_8g087950 | 26.165 | 279 | 165 | 8 | 48 | 292 | 266 | 537 | 9.15e-13 | 72.0 |
| MS.gene071484.t1 | MTR_8g087950 | 26.165 | 279 | 165 | 8 | 48 | 292 | 339 | 610 | 9.16e-13 | 72.0 |
| MS.gene071484.t1 | MTR_1g085410 | 26.515 | 264 | 152 | 8 | 59 | 289 | 9 | 263 | 3.87e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071484.t1 | AT2G31060 | 80.442 | 634 | 119 | 3 | 1 | 630 | 1 | 633 | 0.0 | 1029 |
| MS.gene071484.t1 | AT2G31060 | 79.937 | 638 | 119 | 4 | 1 | 630 | 1 | 637 | 0.0 | 1023 |
| MS.gene071484.t1 | AT2G31060 | 81.744 | 493 | 89 | 1 | 139 | 630 | 1 | 493 | 0.0 | 846 |
| MS.gene071484.t1 | AT5G13650 | 43.140 | 605 | 317 | 10 | 27 | 619 | 47 | 636 | 8.88e-148 | 444 |
| MS.gene071484.t1 | AT5G13650 | 43.234 | 606 | 316 | 11 | 27 | 619 | 47 | 637 | 9.27e-147 | 441 |
| MS.gene071484.t1 | AT5G08650 | 29.367 | 521 | 282 | 22 | 55 | 534 | 84 | 559 | 3.99e-39 | 153 |
| MS.gene071484.t1 | AT5G08650 | 29.367 | 521 | 282 | 22 | 55 | 534 | 84 | 559 | 8.31e-39 | 152 |
| MS.gene071484.t1 | AT5G39900 | 31.148 | 305 | 166 | 9 | 8 | 292 | 9 | 289 | 1.18e-29 | 125 |
| MS.gene071484.t1 | AT2G45030 | 36.702 | 188 | 108 | 3 | 4 | 180 | 10 | 197 | 3.28e-26 | 114 |
| MS.gene071484.t1 | AT3G22980 | 43.511 | 131 | 68 | 1 | 56 | 180 | 8 | 138 | 3.00e-25 | 112 |
| MS.gene071484.t1 | AT3G22980 | 43.511 | 131 | 68 | 1 | 56 | 180 | 8 | 138 | 3.00e-25 | 112 |
| MS.gene071484.t1 | AT3G22980 | 43.511 | 131 | 68 | 1 | 56 | 180 | 8 | 138 | 3.00e-25 | 112 |
| MS.gene071484.t1 | AT1G45332 | 37.097 | 186 | 105 | 4 | 6 | 180 | 13 | 197 | 4.10e-25 | 111 |
| MS.gene071484.t1 | AT1G56070 | 40.816 | 147 | 65 | 4 | 55 | 180 | 17 | 162 | 1.64e-20 | 97.1 |
| MS.gene071484.t1 | AT1G56070 | 40.816 | 147 | 65 | 4 | 55 | 180 | 17 | 162 | 1.64e-20 | 97.1 |
| MS.gene071484.t1 | AT1G56070 | 40.816 | 147 | 65 | 4 | 55 | 180 | 17 | 162 | 1.64e-20 | 97.1 |
| MS.gene071484.t1 | AT3G12915 | 40.136 | 147 | 66 | 4 | 55 | 180 | 17 | 162 | 3.82e-20 | 95.5 |
| MS.gene071484.t1 | AT4G20360 | 30.620 | 258 | 155 | 9 | 59 | 300 | 81 | 330 | 5.08e-20 | 94.0 |
| MS.gene071484.t1 | AT4G20360 | 30.620 | 258 | 155 | 9 | 59 | 300 | 81 | 330 | 5.08e-20 | 94.0 |
| MS.gene071484.t1 | AT1G62750 | 41.085 | 129 | 70 | 2 | 58 | 180 | 97 | 225 | 6.27e-19 | 91.7 |
| MS.gene071484.t1 | AT4G02930 | 28.287 | 251 | 162 | 6 | 59 | 301 | 69 | 309 | 2.96e-17 | 85.5 |
| MS.gene071484.t1 | AT1G06220 | 34.286 | 140 | 80 | 2 | 53 | 180 | 134 | 273 | 3.90e-17 | 86.3 |
| MS.gene071484.t1 | AT1G06220 | 34.286 | 140 | 80 | 2 | 53 | 180 | 134 | 273 | 3.90e-17 | 86.3 |
| MS.gene071484.t1 | AT1G06220 | 34.286 | 140 | 80 | 2 | 53 | 180 | 134 | 273 | 3.90e-17 | 86.3 |
| MS.gene071484.t1 | AT5G25230 | 32.857 | 140 | 82 | 2 | 53 | 180 | 120 | 259 | 6.29e-16 | 82.4 |
| MS.gene071484.t1 | AT5G25230 | 32.857 | 140 | 82 | 2 | 53 | 180 | 120 | 259 | 6.29e-16 | 82.4 |
| MS.gene071484.t1 | AT5G10630 | 25.540 | 278 | 164 | 9 | 59 | 301 | 317 | 586 | 7.86e-13 | 72.0 |
| MS.gene071484.t1 | AT5G10630 | 25.540 | 278 | 164 | 9 | 59 | 301 | 241 | 510 | 8.53e-13 | 72.0 |
| MS.gene071484.t1 | AT5G10630 | 25.540 | 278 | 164 | 9 | 59 | 301 | 242 | 511 | 8.54e-13 | 72.0 |
| MS.gene071484.t1 | AT5G10630 | 25.540 | 278 | 164 | 9 | 59 | 301 | 242 | 511 | 8.54e-13 | 72.0 |
| MS.gene071484.t1 | AT5G10630 | 25.540 | 278 | 164 | 9 | 59 | 301 | 312 | 581 | 9.63e-13 | 72.0 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 31018 | gene | 2328 | 14363 | 2328 | ID=MS.gene071484 |
| 31018 | mRNA | 2328 | 14363 | 2328 | ID=MS.gene071484.t1;Parent=MS.gene071484 |
| 31018 | exon | 2328 | 2648 | 2328 | ID=MS.gene071484.t1.exon1;Parent=MS.gene071484.t1 |
| 31018 | CDS | 2328 | 2648 | 2328 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 2733 | 2807 | 2733 | ID=MS.gene071484.t1.exon2;Parent=MS.gene071484.t1 |
| 31018 | CDS | 2733 | 2807 | 2733 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 4706 | 4856 | 4706 | ID=MS.gene071484.t1.exon3;Parent=MS.gene071484.t1 |
| 31018 | CDS | 4706 | 4856 | 4706 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 6992 | 7060 | 6992 | ID=MS.gene071484.t1.exon4;Parent=MS.gene071484.t1 |
| 31018 | CDS | 6992 | 7060 | 6992 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 7153 | 7325 | 7153 | ID=MS.gene071484.t1.exon5;Parent=MS.gene071484.t1 |
| 31018 | CDS | 7153 | 7325 | 7153 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 7706 | 7843 | 7706 | ID=MS.gene071484.t1.exon6;Parent=MS.gene071484.t1 |
| 31018 | CDS | 7706 | 7843 | 7706 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 8239 | 8364 | 8239 | ID=MS.gene071484.t1.exon7;Parent=MS.gene071484.t1 |
| 31018 | CDS | 8239 | 8364 | 8239 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 9014 | 9112 | 9014 | ID=MS.gene071484.t1.exon8;Parent=MS.gene071484.t1 |
| 31018 | CDS | 9014 | 9112 | 9014 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 9322 | 9442 | 9322 | ID=MS.gene071484.t1.exon9;Parent=MS.gene071484.t1 |
| 31018 | CDS | 9322 | 9442 | 9322 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 9533 | 9596 | 9533 | ID=MS.gene071484.t1.exon10;Parent=MS.gene071484.t1 |
| 31018 | CDS | 9533 | 9596 | 9533 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 10417 | 10471 | 10417 | ID=MS.gene071484.t1.exon11;Parent=MS.gene071484.t1 |
| 31018 | CDS | 10417 | 10471 | 10417 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 10549 | 10673 | 10549 | ID=MS.gene071484.t1.exon12;Parent=MS.gene071484.t1 |
| 31018 | CDS | 10549 | 10673 | 10549 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 12748 | 12821 | 12748 | ID=MS.gene071484.t1.exon13;Parent=MS.gene071484.t1 |
| 31018 | CDS | 12748 | 12821 | 12748 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 13164 | 13213 | 13164 | ID=MS.gene071484.t1.exon14;Parent=MS.gene071484.t1 |
| 31018 | CDS | 13164 | 13213 | 13164 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 13365 | 13451 | 13365 | ID=MS.gene071484.t1.exon15;Parent=MS.gene071484.t1 |
| 31018 | CDS | 13365 | 13451 | 13365 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 13942 | 13992 | 13942 | ID=MS.gene071484.t1.exon16;Parent=MS.gene071484.t1 |
| 31018 | CDS | 13942 | 13992 | 13942 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 14070 | 14153 | 14070 | ID=MS.gene071484.t1.exon17;Parent=MS.gene071484.t1 |
| 31018 | CDS | 14070 | 14153 | 14070 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| 31018 | exon | 14305 | 14363 | 14305 | ID=MS.gene071484.t1.exon18;Parent=MS.gene071484.t1 |
| 31018 | CDS | 14305 | 14363 | 14305 | ID=cds.MS.gene071484.t1;Parent=MS.gene071484.t1 |
| Gene Sequence |
| Protein sequence |
