Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071485.t1 | XP_003607540.2 | 99.8 | 565 | 1 | 0 | 1 | 565 | 74 | 638 | 0.00E+00 | 1108.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071485.t1 | O25225 | 44.8 | 569 | 297 | 6 | 1 | 565 | 21 | 576 | 1.0e-134 | 481.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071485.t1 | G7JUZ7 | 99.8 | 565 | 1 | 0 | 1 | 565 | 74 | 638 | 0.0e+00 | 1108.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene071485 | MS.gene018869 | PPI |
| MS.gene071485 | MS.gene061606 | PPI |
| MS.gene071485 | MS.gene89744 | PPI |
| MS.gene071485 | MS.gene040952 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071485.t1 | MTR_4g079360 | 99.823 | 565 | 1 | 0 | 1 | 565 | 74 | 638 | 0.0 | 1156 |
| MS.gene071485.t1 | MTR_1g052535 | 41.772 | 553 | 308 | 6 | 14 | 565 | 116 | 655 | 4.76e-135 | 408 |
| MS.gene071485.t1 | MTR_1g052535 | 43.316 | 374 | 200 | 4 | 14 | 386 | 116 | 478 | 1.81e-92 | 292 |
| MS.gene071485.t1 | MTR_3g097250 | 27.655 | 499 | 279 | 20 | 2 | 461 | 101 | 556 | 3.23e-26 | 113 |
| MS.gene071485.t1 | MTR_7g009740 | 28.390 | 236 | 127 | 4 | 2 | 219 | 85 | 296 | 1.40e-18 | 90.1 |
| MS.gene071485.t1 | MTR_7g044770 | 44.444 | 90 | 50 | 0 | 18 | 107 | 105 | 194 | 3.49e-16 | 82.4 |
| MS.gene071485.t1 | MTR_4g119340 | 42.391 | 92 | 53 | 0 | 16 | 107 | 52 | 143 | 1.31e-14 | 77.8 |
| MS.gene071485.t1 | MTR_2g021300 | 28.155 | 206 | 134 | 5 | 18 | 219 | 88 | 283 | 2.29e-14 | 75.9 |
| MS.gene071485.t1 | MTR_7g094460 | 26.794 | 209 | 139 | 4 | 18 | 222 | 101 | 299 | 3.22e-13 | 72.4 |
| MS.gene071485.t1 | MTR_2g020660 | 41.758 | 91 | 53 | 0 | 18 | 108 | 98 | 188 | 1.55e-12 | 70.9 |
| MS.gene071485.t1 | MTR_4g101750 | 42.222 | 90 | 52 | 0 | 18 | 107 | 132 | 221 | 1.66e-12 | 70.9 |
| MS.gene071485.t1 | MTR_4g101750 | 42.222 | 90 | 52 | 0 | 18 | 107 | 132 | 221 | 1.67e-12 | 70.9 |
| MS.gene071485.t1 | MTR_2g069050 | 38.889 | 108 | 50 | 2 | 16 | 107 | 55 | 162 | 2.22e-12 | 70.5 |
| MS.gene071485.t1 | MTR_1g048000 | 38.889 | 108 | 50 | 2 | 16 | 107 | 55 | 162 | 2.23e-12 | 70.5 |
| MS.gene071485.t1 | MTR_2g069310 | 37.037 | 108 | 52 | 1 | 16 | 107 | 55 | 162 | 2.42e-12 | 70.5 |
| MS.gene071485.t1 | MTR_2g069320 | 36.111 | 108 | 53 | 1 | 16 | 107 | 55 | 162 | 8.61e-12 | 68.6 |
| MS.gene071485.t1 | MTR_3g076660 | 26.818 | 220 | 145 | 5 | 18 | 227 | 130 | 343 | 1.19e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071485.t1 | AT2G31060 | 85.133 | 565 | 84 | 0 | 1 | 565 | 76 | 640 | 0.0 | 1004 |
| MS.gene071485.t1 | AT2G31060 | 84.534 | 569 | 84 | 1 | 1 | 565 | 76 | 644 | 0.0 | 998 |
| MS.gene071485.t1 | AT2G31060 | 83.600 | 500 | 82 | 0 | 66 | 565 | 1 | 500 | 0.0 | 874 |
| MS.gene071485.t1 | AT5G13650 | 42.807 | 570 | 308 | 7 | 1 | 565 | 99 | 655 | 1.07e-139 | 420 |
| MS.gene071485.t1 | AT5G13650 | 42.807 | 570 | 308 | 7 | 1 | 565 | 100 | 656 | 1.24e-139 | 420 |
| MS.gene071485.t1 | AT5G08650 | 28.099 | 484 | 267 | 20 | 15 | 461 | 120 | 559 | 8.52e-30 | 124 |
| MS.gene071485.t1 | AT5G08650 | 28.099 | 484 | 267 | 20 | 15 | 461 | 120 | 559 | 1.38e-29 | 124 |
| MS.gene071485.t1 | AT5G39900 | 31.455 | 213 | 113 | 5 | 16 | 219 | 101 | 289 | 2.44e-20 | 95.5 |
| MS.gene071485.t1 | AT1G45332 | 44.444 | 90 | 50 | 0 | 18 | 107 | 108 | 197 | 1.71e-16 | 83.6 |
| MS.gene071485.t1 | AT2G45030 | 44.444 | 90 | 50 | 0 | 18 | 107 | 108 | 197 | 1.82e-16 | 83.6 |
| MS.gene071485.t1 | AT3G22980 | 40.179 | 112 | 61 | 1 | 2 | 107 | 27 | 138 | 5.23e-16 | 82.4 |
| MS.gene071485.t1 | AT3G22980 | 40.179 | 112 | 61 | 1 | 2 | 107 | 27 | 138 | 5.23e-16 | 82.4 |
| MS.gene071485.t1 | AT3G22980 | 40.179 | 112 | 61 | 1 | 2 | 107 | 27 | 138 | 5.23e-16 | 82.4 |
| MS.gene071485.t1 | AT4G20360 | 29.279 | 222 | 137 | 7 | 18 | 227 | 117 | 330 | 3.82e-14 | 75.5 |
| MS.gene071485.t1 | AT4G20360 | 29.279 | 222 | 137 | 7 | 18 | 227 | 117 | 330 | 3.82e-14 | 75.5 |
| MS.gene071485.t1 | AT1G62750 | 43.333 | 90 | 51 | 0 | 18 | 107 | 136 | 225 | 1.77e-12 | 70.9 |
| MS.gene071485.t1 | AT1G56070 | 38.889 | 108 | 50 | 2 | 16 | 107 | 55 | 162 | 3.22e-12 | 70.1 |
| MS.gene071485.t1 | AT1G56070 | 38.889 | 108 | 50 | 2 | 16 | 107 | 55 | 162 | 3.22e-12 | 70.1 |
| MS.gene071485.t1 | AT1G56070 | 38.889 | 108 | 50 | 2 | 16 | 107 | 55 | 162 | 3.22e-12 | 70.1 |
| MS.gene071485.t1 | AT4G02930 | 26.887 | 212 | 139 | 5 | 18 | 223 | 105 | 306 | 4.46e-12 | 68.9 |
| MS.gene071485.t1 | AT3G12915 | 37.963 | 108 | 51 | 2 | 16 | 107 | 55 | 162 | 8.73e-12 | 68.6 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 31016 | gene | 733 | 13538 | 733 | ID=MS.gene071485 |
| 31016 | mRNA | 733 | 13538 | 733 | ID=MS.gene071485.t1;Parent=MS.gene071485 |
| 31016 | exon | 733 | 834 | 733 | ID=MS.gene071485.t1.exon1;Parent=MS.gene071485.t1 |
| 31016 | CDS | 733 | 834 | 733 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 919 | 993 | 919 | ID=MS.gene071485.t1.exon2;Parent=MS.gene071485.t1 |
| 31016 | CDS | 919 | 993 | 919 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 2893 | 3043 | 2893 | ID=MS.gene071485.t1.exon3;Parent=MS.gene071485.t1 |
| 31016 | CDS | 2893 | 3043 | 2893 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 5180 | 5248 | 5180 | ID=MS.gene071485.t1.exon4;Parent=MS.gene071485.t1 |
| 31016 | CDS | 5180 | 5248 | 5180 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 5341 | 5513 | 5341 | ID=MS.gene071485.t1.exon5;Parent=MS.gene071485.t1 |
| 31016 | CDS | 5341 | 5513 | 5341 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 5894 | 6031 | 5894 | ID=MS.gene071485.t1.exon6;Parent=MS.gene071485.t1 |
| 31016 | CDS | 5894 | 6031 | 5894 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 6427 | 6552 | 6427 | ID=MS.gene071485.t1.exon7;Parent=MS.gene071485.t1 |
| 31016 | CDS | 6427 | 6552 | 6427 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 7201 | 7299 | 7201 | ID=MS.gene071485.t1.exon8;Parent=MS.gene071485.t1 |
| 31016 | CDS | 7201 | 7299 | 7201 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 7509 | 7629 | 7509 | ID=MS.gene071485.t1.exon9;Parent=MS.gene071485.t1 |
| 31016 | CDS | 7509 | 7629 | 7509 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 7720 | 7783 | 7720 | ID=MS.gene071485.t1.exon10;Parent=MS.gene071485.t1 |
| 31016 | CDS | 7720 | 7783 | 7720 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 8605 | 8659 | 8605 | ID=MS.gene071485.t1.exon11;Parent=MS.gene071485.t1 |
| 31016 | CDS | 8605 | 8659 | 8605 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 8737 | 8861 | 8737 | ID=MS.gene071485.t1.exon12;Parent=MS.gene071485.t1 |
| 31016 | CDS | 8737 | 8861 | 8737 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 10937 | 11010 | 10937 | ID=MS.gene071485.t1.exon13;Parent=MS.gene071485.t1 |
| 31016 | CDS | 10937 | 11010 | 10937 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 11353 | 11402 | 11353 | ID=MS.gene071485.t1.exon14;Parent=MS.gene071485.t1 |
| 31016 | CDS | 11353 | 11402 | 11353 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 11554 | 11640 | 11554 | ID=MS.gene071485.t1.exon15;Parent=MS.gene071485.t1 |
| 31016 | CDS | 11554 | 11640 | 11554 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 12131 | 12181 | 12131 | ID=MS.gene071485.t1.exon16;Parent=MS.gene071485.t1 |
| 31016 | CDS | 12131 | 12181 | 12131 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 12259 | 12342 | 12259 | ID=MS.gene071485.t1.exon17;Parent=MS.gene071485.t1 |
| 31016 | CDS | 12259 | 12342 | 12259 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| 31016 | exon | 13488 | 13538 | 13488 | ID=MS.gene071485.t1.exon18;Parent=MS.gene071485.t1 |
| 31016 | CDS | 13488 | 13538 | 13488 | ID=cds.MS.gene071485.t1;Parent=MS.gene071485.t1 |
| Gene Sequence |
| Protein sequence |
