Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071607.t1 | XP_024629057.1 | 74.6 | 59 | 12 | 1 | 87 | 142 | 118 | 176 | 1.00E-10 | 76.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071607.t1 | G7LB63 | 85.7 | 49 | 4 | 1 | 97 | 142 | 130 | 178 | 2.1e-10 | 75.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052519 | MS.gene071607 | 0.829643 | 4.59E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071607.t1 | MTR_8g075130 | 85.714 | 49 | 4 | 1 | 97 | 142 | 130 | 178 | 4.57e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATATTTTTTTTTTTTTA+GGG | - | 54942:9231-9250 | MS.gene071607:CDS | 0.0% |
| !!! | TATTTTATTAAGTATATGTT+TGG | - | 54942:8862-8881 | MS.gene071607:intron | 10.0% |
| !!! | ACTTTTAGTTTGTTAATTAA+TGG | + | 54942:7791-7810 | MS.gene071607:intergenic | 15.0% |
| !! | AATATTAACCAAAGTACATA+TGG | + | 54942:9219-9238 | MS.gene071607:intergenic | 20.0% |
| !! | GATTAAGAAATTGTATAGTT+TGG | + | 54942:7907-7926 | MS.gene071607:intergenic | 20.0% |
| !! | GGATTGAACTATAAATTTAT+GGG | - | 54942:8996-9015 | MS.gene071607:intron | 20.0% |
| !! | TGGATTGAACTATAAATTTA+TGG | - | 54942:8995-9014 | MS.gene071607:intron | 20.0% |
| !!! | AGAAGCTTTAAAATTGATTT+TGG | + | 54942:9119-9138 | MS.gene071607:intergenic | 20.0% |
| !!! | GCTTTATAACTAATTTTCAT+AGG | + | 54942:7643-7662 | MS.gene071607:intergenic | 20.0% |
| ! | AGAAACTTTACCAAGATAAA+GGG | - | 54942:7675-7694 | MS.gene071607:intron | 25.0% |
| ! | AGTTAAGATAAAGGAAAACT+AGG | + | 54942:7375-7394 | MS.gene071607:intergenic | 25.0% |
| ! | ATTAAGTATATGTTTGGTAC+TGG | - | 54942:8868-8887 | MS.gene071607:intron | 25.0% |
| ! | ATTATGTGTGAGATTATCAT+GGG | - | 54942:7208-7227 | MS.gene071607:intron | 25.0% |
| ! | CTAAATCCTTCTTTGTTAAA+TGG | + | 54942:7709-7728 | MS.gene071607:intergenic | 25.0% |
| ! | GTACTTTGGTTAATATTATC+AGG | - | 54942:9255-9274 | MS.gene071607:CDS | 25.0% |
| ! | GTATAGAATTACTAGTAACA+GGG | + | 54942:8770-8789 | MS.gene071607:intergenic | 25.0% |
| ! | TAGAAACTTTACCAAGATAA+AGG | - | 54942:7674-7693 | MS.gene071607:intron | 25.0% |
| ! | TGTATAGAATTACTAGTAAC+AGG | + | 54942:8771-8790 | MS.gene071607:intergenic | 25.0% |
| ! | TTAAGCCTAAGTTAAGATAA+AGG | + | 54942:7384-7403 | MS.gene071607:intergenic | 25.0% |
| ! | TTTGTTTGGCTGATAATTAA+TGG | + | 54942:8571-8590 | MS.gene071607:intergenic | 25.0% |
| !! | AAAAAGAGATACATGCTTTT+TGG | + | 54942:8043-8062 | MS.gene071607:intergenic | 25.0% |
| !! | CATGGTACTTAATTTTTGTT+TGG | + | 54942:8585-8604 | MS.gene071607:intergenic | 25.0% |
| !! | GTTTTCCTTTATCTTAACTT+AGG | - | 54942:7376-7395 | MS.gene071607:intron | 25.0% |
| !! | TATAGATTTTCGTCAAAACA+TGG | + | 54942:9028-9047 | MS.gene071607:intergenic | 25.0% |
| !! | TATTTACATTTTGTCTGCTT+TGG | - | 54942:7025-7044 | MS.gene071607:CDS | 25.0% |
| !! | TCTTACATATCTTTTTATGC+AGG | - | 54942:7525-7544 | MS.gene071607:intron | 25.0% |
| !! | TTTTTGTGTTTATTTCGTGT+AGG | - | 54942:8652-8671 | MS.gene071607:intron | 25.0% |
| !!! | AAAAGAGATACATGCTTTTT+GGG | + | 54942:8042-8061 | MS.gene071607:intergenic | 25.0% |
| !!! | AATTTGACTTGTCTCTATTT+CGG | - | 54942:8131-8150 | MS.gene071607:intron | 25.0% |
| !!! | AGCTGCTTTTTATAATCTTT+GGG | - | 54942:7161-7180 | MS.gene071607:intron | 25.0% |
| !!! | ATAAGTGTTTTAGAGTTCAA+AGG | - | 54942:7958-7977 | MS.gene071607:intron | 25.0% |
| !!! | CTTTAAAATTGATTTTGGCA+TGG | + | 54942:9114-9133 | MS.gene071607:intergenic | 25.0% |
| !!! | TAAGTGTTTTAGAGTTCAAA+GGG | - | 54942:7959-7978 | MS.gene071607:intron | 25.0% |
| !!! | TTTTTTTTTAGGGTGTACTT+TGG | - | 54942:9241-9260 | MS.gene071607:CDS | 25.0% |
| AAAAAGAATGCAACTTGAAC+TGG | - | 54942:7457-7476 | MS.gene071607:intron | 30.0% | |
| AAAAGGCCATTTAACAAAGA+AGG | - | 54942:7700-7719 | MS.gene071607:intron | 30.0% | |
| AAAATACCATGGAAGATGTT+TGG | - | 54942:8250-8269 | MS.gene071607:intron | 30.0% | |
| AAGGTGATAGTTAACACAAT+TGG | + | 54942:9189-9208 | MS.gene071607:intergenic | 30.0% | |
| AGATTATAAAAAGCAGCTGA+AGG | + | 54942:7159-7178 | MS.gene071607:intergenic | 30.0% | |
| CATAAACTATACACTCAACA+TGG | + | 54942:8603-8622 | MS.gene071607:intergenic | 30.0% | |
| CATTATGTGTGAGATTATCA+TGG | - | 54942:7207-7226 | MS.gene071607:intron | 30.0% | |
| GAAACTTTACCAAGATAAAG+GGG | - | 54942:7676-7695 | MS.gene071607:intron | 30.0% | |
| TATTAAGCAACTCAATAGAG+AGG | - | 54942:8472-8491 | MS.gene071607:intron | 30.0% | |
| TGGATACGTTCATATAAAAG+TGG | - | 54942:8975-8994 | MS.gene071607:intron | 30.0% | |
| TTGTTAGTCCATATGTACTT+TGG | - | 54942:9208-9227 | MS.gene071607:CDS | 30.0% | |
| TTTCAGTTGTAATGTTGAAC+TGG | - | 54942:7931-7950 | MS.gene071607:intron | 30.0% | |
| ! | AACATCTTCCATGGTATTTT+TGG | + | 54942:8250-8269 | MS.gene071607:intergenic | 30.0% |
| ! | AAGTACATATGGACTAACAA+AGG | + | 54942:9208-9227 | MS.gene071607:intergenic | 30.0% |
| ! | ACATCTTCCATGGTATTTTT+GGG | + | 54942:8249-8268 | MS.gene071607:intergenic | 30.0% |
| ! | GTGGTTACTGTTGTTTATAT+GGG | - | 54942:7053-7072 | MS.gene071607:intron | 30.0% |
| ! | TGGTTACTGTTGTTTATATG+GGG | - | 54942:7054-7073 | MS.gene071607:intron | 30.0% |
| !! | AAAATTGATTTTGGCATGGT+TGG | + | 54942:9110-9129 | MS.gene071607:intergenic | 30.0% |
| !! | AAATTGATTTTGGCATGGTT+GGG | + | 54942:9109-9128 | MS.gene071607:intergenic | 30.0% |
| !! | AAGCACTCTTTTATTTGCTA+TGG | + | 54942:8078-8097 | MS.gene071607:intergenic | 30.0% |
| !!! | AAGTGTTTTAGAGTTCAAAG+GGG | - | 54942:7960-7979 | MS.gene071607:intron | 30.0% |
| !!! | CAGCTGCTTTTTATAATCTT+TGG | - | 54942:7160-7179 | MS.gene071607:intron | 30.0% |
| !!! | GCTGCTTTTTATAATCTTTG+GGG | - | 54942:7162-7181 | MS.gene071607:intron | 30.0% |
| AATCAGCAACAAAAGAGGAA+GGG | - | 54942:9429-9448 | MS.gene071607:CDS | 35.0% | |
| ATTACTAGTAACAGGGAAAG+AGG | + | 54942:8763-8782 | MS.gene071607:intergenic | 35.0% | |
| CTTACTTCACACTCACTTTA+GGG | - | 54942:7870-7889 | MS.gene071607:intron | 35.0% | |
| GAAGAAAAAAGTCAAGAGCT+TGG | - | 54942:8691-8710 | MS.gene071607:intron | 35.0% | |
| GTGATAGTTAACACAATTGG+TGG | + | 54942:9186-9205 | MS.gene071607:intergenic | 35.0% | |
| TACCAAGATAAAGGGGTAAA+AGG | - | 54942:7683-7702 | MS.gene071607:intron | 35.0% | |
| TACTGAATGCAACTTGAACT+GGG | - | 54942:7421-7440 | MS.gene071607:intron | 35.0% | |
| TCACACATAATGTTCCAAAG+CGG | + | 54942:7199-7218 | MS.gene071607:intergenic | 35.0% | |
| TCTTACTTCACACTCACTTT+AGG | - | 54942:7869-7888 | MS.gene071607:intron | 35.0% | |
| TTACTTCACACTCACTTTAG+GGG | - | 54942:7871-7890 | MS.gene071607:intron | 35.0% | |
| ! | AACACAATTGGTGGATGTTT+GGG | + | 54942:9177-9196 | MS.gene071607:intergenic | 35.0% |
| ! | AAGGCTGTTTGCTTTAAACT+GGG | - | 54942:7103-7122 | MS.gene071607:intron | 35.0% |
| ! | GGTGGTTACTGTTGTTTATA+TGG | - | 54942:7052-7071 | MS.gene071607:intron | 35.0% |
| ! | TAACACAATTGGTGGATGTT+TGG | + | 54942:9178-9197 | MS.gene071607:intergenic | 35.0% |
| ! | TCAAGTCTTGTCATTTGTGT+TGG | + | 54942:8111-8130 | MS.gene071607:intergenic | 35.0% |
| !! | AAGAGAAGATGGTGTCATTA+AGG | + | 54942:8334-8353 | MS.gene071607:intergenic | 35.0% |
| !! | CATCTTCCATGGTATTTTTG+GGG | + | 54942:8248-8267 | MS.gene071607:intergenic | 35.0% |
| !!! | ATCTTCCATGGTATTTTTGG+GGG | + | 54942:8247-8266 | MS.gene071607:intergenic | 35.0% |
| !!! | TGGATGTTTGGGTTACTTTT+GGG | + | 54942:9166-9185 | MS.gene071607:intergenic | 35.0% |
| AAGAGACTGTGATACTCATG+TGG | + | 54942:8305-8324 | MS.gene071607:intergenic | 40.0% | |
| AAGAGGGTCGAAATGAAACT+CGG | + | 54942:8385-8404 | MS.gene071607:intergenic | 40.0% | |
| ACGATCAAGAGCAAGAAAAC+AGG | - | 54942:8533-8552 | MS.gene071607:intron | 40.0% | |
| ACTGTGATACTCATGTGGTT+TGG | + | 54942:8300-8319 | MS.gene071607:intergenic | 40.0% | |
| CAAGCAATCAGCAACAAAAG+AGG | - | 54942:9424-9443 | MS.gene071607:CDS | 40.0% | |
| CAATCAGCAACAAAAGAGGA+AGG | - | 54942:9428-9447 | MS.gene071607:CDS | 40.0% | |
| GTACTGAATGCAACTTGAAC+TGG | - | 54942:7420-7439 | MS.gene071607:intron | 40.0% | |
| GTCTTTCCAAACATCTTCCA+TGG | + | 54942:8259-8278 | MS.gene071607:intergenic | 40.0% | |
| ! | AGGCTGTTTGCTTTAAACTG+GGG | - | 54942:7104-7123 | MS.gene071607:intron | 40.0% |
| ! | GAAGGCTGTTTGCTTTAAAC+TGG | - | 54942:7102-7121 | MS.gene071607:intron | 40.0% |
| !! | AGAAGATGGTGTCATTAAGG+AGG | + | 54942:8331-8350 | MS.gene071607:intergenic | 40.0% |
| !! | GAAGATGGTGTCATTAAGGA+GGG | + | 54942:8330-8349 | MS.gene071607:intergenic | 40.0% |
| !! | GATACTCATGTGGTTTGGTA+AGG | + | 54942:8295-8314 | MS.gene071607:intergenic | 40.0% |
| !! | GTGGATGTTTGGGTTACTTT+TGG | + | 54942:9167-9186 | MS.gene071607:intergenic | 40.0% |
| !! | GTTGAAGATGGTGTTGTTGT+GGG | + | 54942:8215-8234 | MS.gene071607:intergenic | 40.0% |
| !! | TAGAGAGTTAAGGTTGATGG+TGG | + | 54942:8361-8380 | MS.gene071607:intergenic | 40.0% |
| !! | TCATGTGGTTTGGTAAGGAA+GGG | + | 54942:8290-8309 | MS.gene071607:intergenic | 40.0% |
| !! | TTGAAGATGGTGTTGTTGTG+GGG | + | 54942:8214-8233 | MS.gene071607:intergenic | 40.0% |
| !!! | CTTTTTGGGTATGTGCATTG+TGG | + | 54942:8028-8047 | MS.gene071607:intergenic | 40.0% |
| !!! | GTATTTTTGGGGGGTTTGTT+TGG | + | 54942:8237-8256 | MS.gene071607:intergenic | 40.0% |
| !!! | TCTTCCATGGTATTTTTGGG+GGG | + | 54942:8246-8265 | MS.gene071607:intergenic | 40.0% |
| AAACAGCCTTCCCACAATCA+GGG | + | 54942:7093-7112 | MS.gene071607:intergenic | 45.0% | |
| AAAGCAGCTGAAGGCAATTC+AGG | + | 54942:7150-7169 | MS.gene071607:intergenic | 45.0% | |
| AAGCAGCTGAAGGCAATTCA+GGG | + | 54942:7149-7168 | MS.gene071607:intergenic | 45.0% | |
| AATGTCCCTACCCTGATTGT+GGG | - | 54942:7080-7099 | MS.gene071607:intron | 45.0% | |
| ACTAGTTCACTGAGTGAAGG+TGG | - | 54942:7259-7278 | MS.gene071607:intron | 45.0% | |
| AGAGGGTCGAAATGAAACTC+GGG | + | 54942:8384-8403 | MS.gene071607:intergenic | 45.0% | |
| CAAACCCCCCAAAAATACCA+TGG | - | 54942:8239-8258 | MS.gene071607:intron | 45.0% | |
| CCACACTACTACTGTCTCAA+AGG | - | 54942:8440-8459 | MS.gene071607:intron | 45.0% | |
| GAAACTCGGGTAGAGAGTTA+AGG | + | 54942:8371-8390 | MS.gene071607:intergenic | 45.0% | |
| GCAAGTCATTGTGTGCAAGA+GGG | + | 54942:8401-8420 | MS.gene071607:intergenic | 45.0% | |
| GCTACTAGTTCACTGAGTGA+AGG | - | 54942:7256-7275 | MS.gene071607:intron | 45.0% | |
| GGGTAGAGAGTTAAGGTTGA+TGG | + | 54942:8364-8383 | MS.gene071607:intergenic | 45.0% | |
| TGCAAGTCATTGTGTGCAAG+AGG | + | 54942:8402-8421 | MS.gene071607:intergenic | 45.0% | |
| TTGAGACAGTAGTAGTGTGG+TGG | + | 54942:8440-8459 | MS.gene071607:intergenic | 45.0% | |
| TTTGGGTATGTGCATTGTGG+TGG | + | 54942:8025-8044 | MS.gene071607:intergenic | 45.0% | |
| ! | ATGGTGGAGTGAAGAGAAGA+TGG | + | 54942:8345-8364 | MS.gene071607:intergenic | 45.0% |
| ! | GGCCTTTTACCCCTTTATCT+TGG | + | 54942:7688-7707 | MS.gene071607:intergenic | 45.0% |
| ! | GGGGTTTGTTTGGTTGAAGA+TGG | + | 54942:8227-8246 | MS.gene071607:intergenic | 45.0% |
| ! | TGTGGGGTTTGTTCTTGTAG+AGG | + | 54942:8198-8217 | MS.gene071607:intergenic | 45.0% |
| !! | CCTTTGAGACAGTAGTAGTG+TGG | + | 54942:8443-8462 | MS.gene071607:intergenic | 45.0% |
| !! | CTCATGTGGTTTGGTAAGGA+AGG | + | 54942:8291-8310 | MS.gene071607:intergenic | 45.0% |
| !! | GGTTGAAGATGGTGTTGTTG+TGG | + | 54942:8216-8235 | MS.gene071607:intergenic | 45.0% |
| !!! | CATTTTGTCTGCTTTGGAGC+TGG | - | 54942:7031-7050 | MS.gene071607:CDS | 45.0% |
| !! | AAAACAAAATAATTTATATT+TGG | - | 54942:8955-8974 | MS.gene071607:intron | 5.0% |
| !! | ATAAATAATATTTATAGAAA+TGG | + | 54942:8833-8852 | MS.gene071607:intergenic | 5.0% |
| !! | ATTAATTCTAATTTAAATTT+AGG | + | 54942:9054-9073 | MS.gene071607:intergenic | 5.0% |
| !!! | GATATAATAAATATTTTTAA+CGG | + | 54942:9306-9325 | MS.gene071607:intergenic | 5.0% |
| !!! | GTTAATATTTTTTTTTTTTT+AGG | - | 54942:9230-9249 | MS.gene071607:CDS | 5.0% |
| AGCAGCTGAAGGCAATTCAG+GGG | + | 54942:7148-7167 | MS.gene071607:intergenic | 50.0% | |
| CAAACAGCCTTCCCACAATC+AGG | + | 54942:7094-7113 | MS.gene071607:intergenic | 50.0% | |
| CAATGTCCCTACCCTGATTG+TGG | - | 54942:7079-7098 | MS.gene071607:intron | 50.0% | |
| CACTGAGTGAAGGTGGAAGA+TGG | - | 54942:7266-7285 | MS.gene071607:intron | 50.0% | |
| CAGGGGAATGCCAAAACACA+GGG | + | 54942:7131-7150 | MS.gene071607:intergenic | 50.0% | |
| TCAGGGGAATGCCAAAACAC+AGG | + | 54942:7132-7151 | MS.gene071607:intergenic | 50.0% | |
| ! | AAACTGGGGACCCTGTGTTT+TGG | - | 54942:7118-7137 | MS.gene071607:intron | 50.0% |
| !! | TGTGGTTTGGTAAGGAAGGG+AGG | + | 54942:8287-8306 | MS.gene071607:intergenic | 50.0% |
| !! | TTTGTCTGCTTTGGAGCTGG+TGG | - | 54942:7034-7053 | MS.gene071607:CDS | 50.0% |
| AGCCTTCCCACAATCAGGGT+AGG | + | 54942:7089-7108 | MS.gene071607:intergenic | 55.0% | |
| GCCTTCCCACAATCAGGGTA+GGG | + | 54942:7088-7107 | MS.gene071607:intergenic | 55.0% | |
| GGGTCATCTTCTCACCGCTT+TGG | - | 54942:7182-7201 | MS.gene071607:intron | 55.0% | |
| TCCCTACCCTGATTGTGGGA+AGG | - | 54942:7084-7103 | MS.gene071607:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 54942 | gene | 7004 | 9485 | 7004 | ID=MS.gene071607 |
| 54942 | mRNA | 7004 | 9485 | 7004 | ID=MS.gene071607.t1;Parent=MS.gene071607 |
| 54942 | exon | 9198 | 9485 | 9198 | ID=MS.gene071607.t1.exon1;Parent=MS.gene071607.t1 |
| 54942 | CDS | 9198 | 9485 | 9198 | ID=cds.MS.gene071607.t1;Parent=MS.gene071607.t1 |
| 54942 | exon | 7741 | 7815 | 7741 | ID=MS.gene071607.t1.exon2;Parent=MS.gene071607.t1 |
| 54942 | CDS | 7741 | 7815 | 7741 | ID=cds.MS.gene071607.t1;Parent=MS.gene071607.t1 |
| 54942 | exon | 7004 | 7069 | 7004 | ID=MS.gene071607.t1.exon3;Parent=MS.gene071607.t1 |
| 54942 | CDS | 7004 | 7069 | 7004 | ID=cds.MS.gene071607.t1;Parent=MS.gene071607.t1 |
| Gene Sequence |
| Protein sequence |
