AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071704

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071704.t1 MTR_7g118170 98.134 536 8 1 1 536 1 534 0.0 1085
MS.gene071704.t1 MTR_1g076430 64.741 502 166 5 30 531 17 507 0.0 672
MS.gene071704.t1 MTR_1g041085 65.139 502 164 5 30 531 23 513 0.0 664
MS.gene071704.t1 MTR_2g054940 65.269 501 163 4 31 531 17 506 0.0 663
MS.gene071704.t1 MTR_4g113280 62.874 501 164 4 31 531 6 484 0.0 637
MS.gene071704.t1 MTR_4g070270 60.792 505 184 5 27 530 11 502 0.0 631
MS.gene071704.t1 MTR_7g081695 62.800 500 157 7 31 527 17 490 0.0 619
MS.gene071704.t1 MTR_3g105550 55.664 512 212 7 27 530 32 536 0.0 596
MS.gene071704.t1 MTR_1g110930 57.800 500 197 6 33 531 5 491 0.0 592
MS.gene071704.t1 MTR_5g056510 57.058 503 196 6 33 531 5 491 0.0 576
MS.gene071704.t1 MTR_7g118160 61.822 461 128 4 70 528 3 417 0.0 570
MS.gene071704.t1 MTR_4g054630 54.390 467 205 4 67 532 9 468 0.0 521
MS.gene071704.t1 MTR_4g054415 57.882 425 173 3 109 532 8 427 0.0 520
MS.gene071704.t1 MTR_6g074880 56.481 432 177 4 100 530 47 468 1.61e-179 513
MS.gene071704.t1 MTR_6g032965 58.312 391 155 3 146 533 2 387 4.86e-166 476
MS.gene071704.t1 MTR_2g013740 49.293 495 228 5 27 519 27 500 7.53e-166 480
MS.gene071704.t1 MTR_2g007020 51.923 416 192 4 118 532 45 453 1.24e-155 452
MS.gene071704.t1 MTR_2g436480 48.605 430 209 5 105 530 30 451 9.88e-148 432
MS.gene071704.t1 MTR_1g102290 46.229 411 217 2 118 528 78 484 2.16e-133 397
MS.gene071704.t1 MTR_3g072940 45.476 420 218 5 114 530 74 485 2.94e-127 381
MS.gene071704.t1 MTR_3g072980 45.476 420 218 5 114 530 74 485 3.04e-127 381
MS.gene071704.t1 MTR_4g062270 43.256 430 225 7 106 527 15 433 3.53e-123 369
MS.gene071704.t1 MTR_2g096210 43.237 451 233 8 105 536 4 450 6.88e-123 369
MS.gene071704.t1 MTR_4g062170 42.661 436 227 6 106 527 15 441 9.87e-120 361
MS.gene071704.t1 MTR_4g062190 41.972 436 231 5 106 527 15 442 5.49e-119 359
MS.gene071704.t1 MTR_6g051440 45.631 412 213 5 121 529 80 483 1.48e-118 358
MS.gene071704.t1 MTR_1g115560 40.455 440 238 5 107 527 17 451 6.42e-118 357
MS.gene071704.t1 MTR_4g054260 53.992 263 98 4 271 532 87 327 4.10e-93 287
MS.gene071704.t1 MTR_4g053905 58.434 166 64 2 367 532 1 161 1.24e-63 205
MS.gene071704.t1 MTR_4g054265 40.708 113 67 0 107 219 6 118 1.26e-24 99.4
MS.gene071704.t1 MTR_4g078730 26.102 295 178 12 206 495 120 379 2.17e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071704.t1 AT1G01120 73.157 529 133 5 4 530 5 526 0.0 800
MS.gene071704.t1 AT2G26640 64.881 504 160 5 31 531 15 504 0.0 673
MS.gene071704.t1 AT1G04220 64.103 507 166 7 31 531 20 516 0.0 662
MS.gene071704.t1 AT1G19440 60.396 505 186 5 27 530 23 514 0.0 627
MS.gene071704.t1 AT5G43760 63.380 497 169 5 39 531 34 521 0.0 622
MS.gene071704.t1 AT2G16280 60.355 507 177 9 30 530 22 510 0.0 616
MS.gene071704.t1 AT2G26250 54.753 526 204 7 27 528 33 548 0.0 586
MS.gene071704.t1 AT1G68530 56.151 504 199 7 33 531 6 492 0.0 582
MS.gene071704.t1 AT4G34510 58.149 497 186 8 37 530 8 485 0.0 578
MS.gene071704.t1 AT2G46720 62.471 429 158 1 102 530 40 465 0.0 577
MS.gene071704.t1 AT1G25450 56.112 499 207 5 33 531 1 487 0.0 571
MS.gene071704.t1 AT3G10280 61.305 429 156 2 102 530 40 458 0.0 559
MS.gene071704.t1 AT2G15090 51.660 482 219 3 59 532 6 481 0.0 522
MS.gene071704.t1 AT4G34250 56.787 442 174 5 104 534 56 491 0.0 519
MS.gene071704.t1 AT4G34520 56.787 442 171 5 104 530 54 490 1.81e-179 515
MS.gene071704.t1 AT1G71160 50.116 431 206 3 106 535 31 453 5.12e-156 453
MS.gene071704.t1 AT5G49070 46.405 459 234 6 82 533 5 458 3.92e-147 431
MS.gene071704.t1 AT3G52160 45.763 413 188 3 108 520 74 450 2.78e-132 392
MS.gene071704.t1 AT3G52160 47.978 371 157 3 150 520 3 337 2.34e-123 365
MS.gene071704.t1 AT1G68530 47.244 381 185 5 33 408 6 375 4.19e-122 363
MS.gene071704.t1 AT2G28630 41.958 429 237 4 110 527 19 446 3.94e-121 365
MS.gene071704.t1 AT2G28630 41.958 429 237 4 110 527 19 446 3.94e-121 365
MS.gene071704.t1 AT5G04530 41.355 428 237 6 107 527 17 437 3.37e-117 354
MS.gene071704.t1 AT1G07720 42.991 428 223 6 116 527 25 447 2.66e-116 352
MS.gene071704.t1 AT1G07720 42.991 428 223 6 116 527 68 490 4.26e-116 353
MS.gene071704.t1 AT5G49070 42.071 309 173 3 82 384 5 313 1.63e-82 260

Find 135 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGTCTCGTCTTCTTTC+TGG 0.110532 7.3:-472739 None:intergenic
TCTTGTCATCAGCTCCTTTA+TGG 0.143442 7.3:-472700 None:intergenic
TGTATGAACCAGCTTGTATT+TGG 0.221689 7.3:-472669 None:intergenic
AAGACCACCGCGTAGGAATT+TGG 0.226830 7.3:-472518 None:intergenic
TTCATTGGCTAGAGAATTAA+TGG 0.254813 7.3:+472772 MS.gene071704:CDS
AACATGAACTGTTCAGAAAA+TGG 0.259600 7.3:-472851 None:intergenic
CAAAATCCGACGACGTTTAC+CGG 0.274993 7.3:+471773 MS.gene071704:CDS
GGTTCGATCAGAGGACTTAT+TGG 0.284693 7.3:-472642 None:intergenic
CTTGTCATCAGCTCCTTTAT+GGG 0.289862 7.3:-472699 None:intergenic
AATTTGAAATCTGGTATATA+AGG 0.292144 7.3:-472929 None:intergenic
ATTTGCTTTCAAAAGATCTT+TGG 0.295644 7.3:-472495 None:intergenic
GCAAAATCCACCAGGTAAAT+TGG 0.298744 7.3:-472053 None:intergenic
GGGATTGGCGGGGCAACCCC+TGG 0.302657 7.3:+473227 MS.gene071704:CDS
GAAGCAGAAGCTGTAATGTT+TGG 0.314657 7.3:+472275 MS.gene071704:CDS
TCAAAAGCTAATTTGAAATC+TGG 0.331655 7.3:-472938 None:intergenic
CGGGTCTGGCAGATTGCATT+CGG 0.336102 7.3:+473145 MS.gene071704:CDS
TTGCAAAAGGGGATCGGGTC+TGG 0.336451 7.3:+473131 MS.gene071704:CDS
TGCGCGACTTGCCCGTCGTC+GGG 0.341272 7.3:+473206 MS.gene071704:CDS
GCCCGTCGTCGGGGATTGGC+GGG 0.352548 7.3:+473216 MS.gene071704:CDS
TTAACCCTCAATTGGTACTT+TGG 0.358565 7.3:+472554 MS.gene071704:CDS
GGACTTGACATTATTCCATT+TGG 0.369284 7.3:-472221 None:intergenic
CGGATGACTCTGCATAGATT+TGG 0.389216 7.3:+473049 MS.gene071704:CDS
GCTTCTTTCATGCATAGATT+TGG 0.389782 7.3:-472245 None:intergenic
GCAAAGGGCCGGGTTGCAAA+AGG 0.390952 7.3:+473118 MS.gene071704:CDS
AGAGAATTAATGGCTGTTGC+AGG 0.392253 7.3:+472782 MS.gene071704:CDS
GTTAAAATGTTAGAAGATAG+TGG 0.394444 7.3:+472122 MS.gene071704:CDS
AGGGAAAGTTGGTGTTTCAT+TGG 0.396299 7.3:+472757 MS.gene071704:CDS
ACACTTCAAGCAGTTCTCTA+TGG 0.396346 7.3:+473074 MS.gene071704:CDS
ACTTGCCCGTCGTCGGGGAT+TGG 0.409147 7.3:+473212 MS.gene071704:CDS
AAGCTATAATCTCGGAGGTA+TGG 0.411775 7.3:+472439 MS.gene071704:CDS
AAAACAAACATAACAACGTT+GGG 0.411988 7.3:+472815 MS.gene071704:CDS
TTAGAAGATAGTGGCGAATT+CGG 0.414252 7.3:+472131 MS.gene071704:CDS
AAACTCAAGTACGTAAAGTT+AGG 0.423051 7.3:+471813 MS.gene071704:CDS
CATAACAACGTTGGGTCCAT+TGG 0.424394 7.3:+472823 MS.gene071704:CDS
CCAACTCAGTGAGTGGCATA+TGG 0.428746 7.3:+473018 MS.gene071704:CDS
TGGCAGATTGCATTCGGGTC+GGG 0.430711 7.3:+473151 MS.gene071704:CDS
GGAGGTATGGGTTGTAGTGC+TGG 0.432856 7.3:+472452 MS.gene071704:CDS
CGGAGAATTTAACCCTCAAT+TGG 0.433036 7.3:+472546 MS.gene071704:CDS
ATTGTTCTACTCTTCCTCTT+CGG 0.433972 7.3:+472005 MS.gene071704:CDS
TTGCTGCTGAAGAAAGGTTC+AGG 0.437231 7.3:+472899 MS.gene071704:CDS
CCGGGTTGCAAAAGGGGATC+GGG 0.444082 7.3:+473126 MS.gene071704:CDS
CTGGCAGATTGCATTCGGGT+CGG 0.444799 7.3:+473150 MS.gene071704:CDS
GCCGGGTTGCAAAAGGGGAT+CGG 0.445235 7.3:+473125 MS.gene071704:CDS
CAGTTCTCTATGGTATGAAC+TGG 0.451448 7.3:+473084 MS.gene071704:CDS
CATACAGTTAGAACCCATAA+AGG 0.451862 7.3:+472686 MS.gene071704:CDS
CAAAGGGCCGGGTTGCAAAA+GGG 0.466708 7.3:+473119 MS.gene071704:CDS
ACAAGAATGTCAATGTCTCT+TGG 0.469200 7.3:-472335 None:intergenic
GTATAGCGGCGCCACCCATT+CGG 0.470845 7.3:-472613 None:intergenic
TCTTTCATGCATAGATTTGG+TGG 0.474801 7.3:-472242 None:intergenic
TTGCAGAAAGTGAAGGAGTT+GGG 0.476301 7.3:-472379 None:intergenic
GTGCGCGACTTGCCCGTCGT+CGG 0.478086 7.3:+473205 MS.gene071704:CDS
CATTTCCAAAGTACCAATTG+AGG 0.478571 7.3:-472559 None:intergenic
GGATTGGCGGGGCAACCCCT+GGG 0.483271 7.3:+473228 MS.gene071704:CDS
TGCCCGTCGTCGGGGATTGG+CGG 0.485832 7.3:+473215 MS.gene071704:CDS
AGCTATAATCTCGGAGGTAT+GGG 0.488082 7.3:+472440 MS.gene071704:CDS
GAACCAGCTTGTATTTGGAC+CGG 0.488419 7.3:-472664 None:intergenic
AAGTACGTAAAGTTAGGTTA+TGG 0.495016 7.3:+471819 MS.gene071704:CDS
GAACATTTCTGTATTCATGC+TGG 0.495485 7.3:+472959 MS.gene071704:CDS
CCCCGCCAATCCCCGACGAC+GGG 0.497979 7.3:-473217 None:intergenic
TTCACCGTCTGTAGAAAATC+CGG 0.498288 7.3:-471792 None:intergenic
AGGAAGTTGCTGCTGAAGAA+AGG 0.501320 7.3:+472893 MS.gene071704:CDS
AAAGGGCCGGGTTGCAAAAG+GGG 0.505317 7.3:+473120 MS.gene071704:CDS
ATTTCCAAAGTACCAATTGA+GGG 0.506557 7.3:-472558 None:intergenic
GGGTCTGGCAGATTGCATTC+GGG 0.514216 7.3:+473146 MS.gene071704:CDS
AATTGAACCGATGGCTGTAT+CGG 0.514947 7.3:-471985 None:intergenic
GACGAAACTTCCTTGCCAAA+TGG 0.515853 7.3:+472206 MS.gene071704:CDS
TTATACTGAGGCAAAGGGCC+GGG 0.516750 7.3:+473108 MS.gene071704:CDS
GAAAACAAACATAACAACGT+TGG 0.519196 7.3:+472814 MS.gene071704:CDS
TGTTTGTTCTTAGCTTGTAG+TGG 0.521220 7.3:-472412 None:intergenic
ACAGAAACAGGGACGCTGAC+GGG 0.521485 7.3:-473271 None:intergenic
AACATTAAAAGCTATAATCT+CGG 0.522267 7.3:+472431 MS.gene071704:CDS
CTTATACTGAGGCAAAGGGC+CGG 0.522593 7.3:+473107 MS.gene071704:CDS
CCCGTCGTCGGGGATTGGCG+GGG 0.523629 7.3:+473217 MS.gene071704:CDS
TACCAGAAAGAAGACGAGAC+AGG 0.524700 7.3:+472737 MS.gene071704:CDS
AATCCACCAGGTAAATTGGT+GGG 0.541242 7.3:-472049 None:intergenic
ATTGCAGAAAGTGAAGGAGT+TGG 0.552174 7.3:-472380 None:intergenic
GAAGACGAGACAGGGAAAGT+TGG 0.555142 7.3:+472746 MS.gene071704:CDS
TCAGAAAATGGCAAAACCAA+TGG 0.557047 7.3:-472839 None:intergenic
GCCCCGCCAATCCCCGACGA+CGG 0.559697 7.3:-473218 None:intergenic
ACGATCCCCACCAATTTACC+TGG 0.560276 7.3:+472043 MS.gene071704:CDS
TTTGGACCGGGTTCGATCAG+AGG 0.562259 7.3:-472651 None:intergenic
CCATATGCCACTCACTGAGT+TGG 0.569030 7.3:-473018 None:intergenic
GCAAGAGTTGTGAGAGACGG+TGG 0.571620 7.3:-471938 None:intergenic
TGGCTGTATCGGTGTCAAGT+TGG 0.573119 7.3:-471974 None:intergenic
GACGACGGGCAAGTCGCGCA+CGG 0.573305 7.3:-473203 None:intergenic
ACTGGCTTATACTGAGGCAA+AGG 0.574125 7.3:+473102 MS.gene071704:CDS
AACCAGCTTGTATTTGGACC+GGG 0.576851 7.3:-472663 None:intergenic
CTTATCAACAGAGTCATCCC+AGG 0.582184 7.3:-473245 None:intergenic
ATAAGTCCTCTGATCGAACC+CGG 0.585371 7.3:+472645 MS.gene071704:CDS
ATCCACCAGGTAAATTGGTG+GGG 0.585932 7.3:-472048 None:intergenic
AACCCGGTCCAAATACAAGC+TGG 0.588921 7.3:+472661 MS.gene071704:CDS
TGACATTATTCCATTTGGCA+AGG 0.590067 7.3:-472216 None:intergenic
CTGGCTTATACTGAGGCAAA+GGG 0.590480 7.3:+473103 MS.gene071704:CDS
CATTTCTGTATTCATGCTGG+TGG 0.590993 7.3:+472962 MS.gene071704:CDS
AACAGAAACAGGGACGCTGA+CGG 0.594758 7.3:-473272 None:intergenic
GAGTAGAACAATTGAACCGA+TGG 0.597177 7.3:-471994 None:intergenic
AAATCCACCAGGTAAATTGG+TGG 0.601519 7.3:-472050 None:intergenic
AACCTGCTCTTGACAAAATG+CGG 0.602486 7.3:-472178 None:intergenic
GTATGAACTGGCTTATACTG+AGG 0.604352 7.3:+473096 MS.gene071704:CDS
TGTGAAAATGAGAATGGTTG+TGG 0.605218 7.3:-471856 None:intergenic
ATCCCCACCAATTTACCTGG+TGG 0.607799 7.3:+472046 MS.gene071704:CDS
ATCGGTGAGTTGGAACTGAA+CGG 0.608509 7.3:-471907 None:intergenic
ACAATCATTGCAGAAAGTGA+AGG 0.609013 7.3:-472386 None:intergenic
ACCAGAAAGAAGACGAGACA+GGG 0.610150 7.3:+472738 MS.gene071704:CDS
GTGTAACAGTGCTGTGTGGA+AGG 0.610162 7.3:+473180 MS.gene071704:CDS
TTTAAGAAGCAACAGAAACA+GGG 0.614888 7.3:-473282 None:intergenic
CGGTGAGGAAGAGTAAGAGA+GGG 0.614974 7.3:-471887 None:intergenic
GCGCGACTTGCCCGTCGTCG+GGG 0.623964 7.3:+473207 MS.gene071704:CDS
CGAGCAAGAGTTGTGAGAGA+CGG 0.627180 7.3:-471941 None:intergenic
GACGAGTGTGAAAATGAGAA+TGG 0.628164 7.3:-471862 None:intergenic
ACGGTGAGGAAGAGTAAGAG+AGG 0.633167 7.3:-471888 None:intergenic
CTACGCGGTGGTCTTGAGCA+CGG 0.635511 7.3:+472526 MS.gene071704:CDS
GAAAATCCGGTAAACGTCGT+CGG 0.635960 7.3:-471779 None:intergenic
GCTACAAACCGGAGAAAGAG+CGG 0.638965 7.3:+472078 MS.gene071704:CDS
AGTGGCATATGGAACCGTCT+CGG 0.641136 7.3:+473029 MS.gene071704:CDS
TGTAGCACGCAAAATCCACC+AGG 0.643428 7.3:-472061 None:intergenic
CTTGACACCGATACAGCCAT+CGG 0.644102 7.3:+471978 MS.gene071704:CDS
GGGTCGCAACAGCATAGACA+TGG 0.648812 7.3:+471695 MS.gene071704:CDS
TTATCAACAGAGTCATCCCA+GGG 0.649243 7.3:-473244 None:intergenic
AGAATCTCCAACTCAGTGAG+TGG 0.655119 7.3:+473011 MS.gene071704:CDS
TGTATCGGTGTCAAGTTGGA+CGG 0.658526 7.3:-471970 None:intergenic
CTATGCAGAGTCATCCGAGA+CGG 0.659543 7.3:-473043 None:intergenic
CTTATTGGAAAGAAGTATAG+CGG 0.661996 7.3:-472627 None:intergenic
CATGGATAAAGAAAGACTAA+CGG 0.662335 7.3:+471713 MS.gene071704:CDS
AAATCCAAATTCCTACGCGG+TGG 0.667104 7.3:+472514 MS.gene071704:CDS
GAAAGAGCGGAAAATAGCAG+TGG 0.668978 7.3:+472091 MS.gene071704:CDS
CGTGCTCAAGACCACCGCGT+AGG 0.672026 7.3:-472525 None:intergenic
ATTAAAAGCTATAATCTCGG+AGG 0.675727 7.3:+472434 MS.gene071704:CDS
TCGCGTAGTACAGACCGAAG+AGG 0.689821 7.3:-472019 None:intergenic
TTTCAAAGATTCAACCTCCG+CGG 0.705846 7.3:+471746 MS.gene071704:CDS
TGAGTTGGAACTGAACGGTG+AGG 0.720930 7.3:-471902 None:intergenic
TTAAGTGTAACAGTGCTGTG+TGG 0.723760 7.3:+473176 MS.gene071704:CDS
GGATAAAGAAAGACTAACGG+CGG 0.724763 7.3:+471716 MS.gene071704:CDS
AGCAAATCCAAATTCCTACG+CGG 0.758527 7.3:+472511 MS.gene071704:CDS
TATCAACAGAGTCATCCCAG+GGG 0.818112 7.3:-473243 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATTAAAAGCTATAATCT+CGG + chr7.3:472431-472450 MS.gene071704:CDS 20.0%
!!! AATTTGAAATCTGGTATATA+AGG - chr7.3:472932-472951 None:intergenic 20.0%
! AAAACAAACATAACAACGTT+GGG + chr7.3:472815-472834 MS.gene071704:CDS 25.0%
! ATTTGCTTTCAAAAGATCTT+TGG - chr7.3:472498-472517 None:intergenic 25.0%
! GTTAAAATGTTAGAAGATAG+TGG + chr7.3:472122-472141 MS.gene071704:CDS 25.0%
!! TCAAAAGCTAATTTGAAATC+TGG - chr7.3:472941-472960 None:intergenic 25.0%
!! TTTTTGTGTCTTTGGTTAAA+AGG + chr7.3:472873-472892 MS.gene071704:CDS 25.0%
AAACTCAAGTACGTAAAGTT+AGG + chr7.3:471813-471832 MS.gene071704:CDS 30.0%
AACATGAACTGTTCAGAAAA+TGG - chr7.3:472854-472873 None:intergenic 30.0%
AAGTACGTAAAGTTAGGTTA+TGG + chr7.3:471819-471838 MS.gene071704:CDS 30.0%
ATTAAAAGCTATAATCTCGG+AGG + chr7.3:472434-472453 MS.gene071704:CDS 30.0%
CATGGATAAAGAAAGACTAA+CGG + chr7.3:471713-471732 MS.gene071704:CDS 30.0%
GAAAACAAACATAACAACGT+TGG + chr7.3:472814-472833 MS.gene071704:CDS 30.0%
! ATTTCCAAAGTACCAATTGA+GGG - chr7.3:472561-472580 None:intergenic 30.0%
! CTTATTGGAAAGAAGTATAG+CGG - chr7.3:472630-472649 None:intergenic 30.0%
! TCTAATTGCATTTTCCGAAT+GGG + chr7.3:472599-472618 MS.gene071704:CDS 30.0%
! TTCATTGGCTAGAGAATTAA+TGG + chr7.3:472772-472791 MS.gene071704:CDS 30.0%
! TTCTAATTGCATTTTCCGAA+TGG + chr7.3:472598-472617 MS.gene071704:CDS 30.0%
!!! GTTCATGTTTTTTGTGTCTT+TGG + chr7.3:472865-472884 MS.gene071704:CDS 30.0%
ACAAGAATGTCAATGTCTCT+TGG - chr7.3:472338-472357 None:intergenic 35.0%
ACAATCATTGCAGAAAGTGA+AGG - chr7.3:472389-472408 None:intergenic 35.0%
ATTGTTCTACTCTTCCTCTT+CGG + chr7.3:472005-472024 MS.gene071704:CDS 35.0%
CATACAGTTAGAACCCATAA+AGG + chr7.3:472686-472705 MS.gene071704:CDS 35.0%
GAACATTTCTGTATTCATGC+TGG + chr7.3:472959-472978 MS.gene071704:CDS 35.0%
GCTTCTTTCATGCATAGATT+TGG - chr7.3:472248-472267 None:intergenic 35.0%
GGACTTGACATTATTCCATT+TGG - chr7.3:472224-472243 None:intergenic 35.0%
TCAGAAAATGGCAAAACCAA+TGG - chr7.3:472842-472861 None:intergenic 35.0%
TCTTTCATGCATAGATTTGG+TGG - chr7.3:472245-472264 None:intergenic 35.0%
TGACATTATTCCATTTGGCA+AGG - chr7.3:472219-472238 None:intergenic 35.0%
TGTATGAACCAGCTTGTATT+TGG - chr7.3:472672-472691 None:intergenic 35.0%
TGTGAAAATGAGAATGGTTG+TGG - chr7.3:471859-471878 None:intergenic 35.0%
TTAACCCTCAATTGGTACTT+TGG + chr7.3:472554-472573 MS.gene071704:CDS 35.0%
! ATTTTGTCAAGAGCAGGTTT+AGG + chr7.3:472182-472201 MS.gene071704:CDS 35.0%
! CATTTCCAAAGTACCAATTG+AGG - chr7.3:472562-472581 None:intergenic 35.0%
! TTAGAAGATAGTGGCGAATT+CGG + chr7.3:472131-472150 MS.gene071704:CDS 35.0%
! TTTACCGGATTTTCTACAGA+CGG + chr7.3:471788-471807 MS.gene071704:CDS 35.0%
!! TGTTTGTTCTTAGCTTGTAG+TGG - chr7.3:472415-472434 None:intergenic 35.0%
AAATCCACCAGGTAAATTGG+TGG - chr7.3:472053-472072 None:intergenic 40.0%
AACCTGCTCTTGACAAAATG+CGG - chr7.3:472181-472200 None:intergenic 40.0%
AAGCTATAATCTCGGAGGTA+TGG + chr7.3:472439-472458 MS.gene071704:CDS 40.0%
AATCCACCAGGTAAATTGGT+GGG - chr7.3:472052-472071 None:intergenic 40.0%
AATTGAACCGATGGCTGTAT+CGG - chr7.3:471988-472007 None:intergenic 40.0%
ACACTTCAAGCAGTTCTCTA+TGG + chr7.3:473074-473093 MS.gene071704:CDS 40.0%
AGAGAATTAATGGCTGTTGC+AGG + chr7.3:472782-472801 MS.gene071704:CDS 40.0%
AGCAAATCCAAATTCCTACG+CGG + chr7.3:472511-472530 MS.gene071704:CDS 40.0%
ATTGCAGAAAGTGAAGGAGT+TGG - chr7.3:472383-472402 None:intergenic 40.0%
CAGTTCTCTATGGTATGAAC+TGG + chr7.3:473084-473103 MS.gene071704:CDS 40.0%
CATTTCTGTATTCATGCTGG+TGG + chr7.3:472962-472981 MS.gene071704:CDS 40.0%
CGGAGAATTTAACCCTCAAT+TGG + chr7.3:472546-472565 MS.gene071704:CDS 40.0%
CTTGTCATCAGCTCCTTTAT+GGG - chr7.3:472702-472721 None:intergenic 40.0%
GAAGCAGAAGCTGTAATGTT+TGG + chr7.3:472275-472294 MS.gene071704:CDS 40.0%
GACGAGTGTGAAAATGAGAA+TGG - chr7.3:471865-471884 None:intergenic 40.0%
GAGTAGAACAATTGAACCGA+TGG - chr7.3:471997-472016 None:intergenic 40.0%
GCAAAATCCACCAGGTAAAT+TGG - chr7.3:472056-472075 None:intergenic 40.0%
GGATAAAGAAAGACTAACGG+CGG + chr7.3:471716-471735 MS.gene071704:CDS 40.0%
TCTTGTCATCAGCTCCTTTA+TGG - chr7.3:472703-472722 None:intergenic 40.0%
TTAAGTGTAACAGTGCTGTG+TGG + chr7.3:473176-473195 MS.gene071704:CDS 40.0%
TTATCAACAGAGTCATCCCA+GGG - chr7.3:473247-473266 None:intergenic 40.0%
TTCACCGTCTGTAGAAAATC+CGG - chr7.3:471795-471814 None:intergenic 40.0%
TTGCAGAAAGTGAAGGAGTT+GGG - chr7.3:472382-472401 None:intergenic 40.0%
TTTCAAAGATTCAACCTCCG+CGG + chr7.3:471746-471765 MS.gene071704:CDS 40.0%
! AATTGCATTTTCCGAATGGG+TGG + chr7.3:472602-472621 MS.gene071704:CDS 40.0%
! AGCTATAATCTCGGAGGTAT+GGG + chr7.3:472440-472459 MS.gene071704:CDS 40.0%
! GTATGAACTGGCTTATACTG+AGG + chr7.3:473096-473115 MS.gene071704:CDS 40.0%
!! AGGGAAAGTTGGTGTTTCAT+TGG + chr7.3:472757-472776 MS.gene071704:CDS 40.0%
!! GACGCACTTTTTGCTAAAAC+AGG + chr7.3:472305-472324 MS.gene071704:CDS 40.0%
AAATCCAAATTCCTACGCGG+TGG + chr7.3:472514-472533 MS.gene071704:CDS 45.0%
AACCAGCTTGTATTTGGACC+GGG - chr7.3:472666-472685 None:intergenic 45.0%
ACCAGAAAGAAGACGAGACA+GGG + chr7.3:472738-472757 MS.gene071704:CDS 45.0%
AGAATCTCCAACTCAGTGAG+TGG + chr7.3:473011-473030 MS.gene071704:CDS 45.0%
AGGAAGTTGCTGCTGAAGAA+AGG + chr7.3:472893-472912 MS.gene071704:CDS 45.0%
ATAAGTCCTCTGATCGAACC+CGG + chr7.3:472645-472664 MS.gene071704:CDS 45.0%
ATCCACCAGGTAAATTGGTG+GGG - chr7.3:472051-472070 None:intergenic 45.0%
CAAAATCCGACGACGTTTAC+CGG + chr7.3:471773-471792 MS.gene071704:CDS 45.0%
CATAACAACGTTGGGTCCAT+TGG + chr7.3:472823-472842 MS.gene071704:CDS 45.0%
CGGATGACTCTGCATAGATT+TGG + chr7.3:473049-473068 MS.gene071704:CDS 45.0%
CTGGCTTATACTGAGGCAAA+GGG + chr7.3:473103-473122 MS.gene071704:CDS 45.0%
CTTATCAACAGAGTCATCCC+AGG - chr7.3:473248-473267 None:intergenic 45.0%
GAAAATCCGGTAAACGTCGT+CGG - chr7.3:471782-471801 None:intergenic 45.0%
GAAAGAGCGGAAAATAGCAG+TGG + chr7.3:472091-472110 MS.gene071704:CDS 45.0%
GAACCAGCTTGTATTTGGAC+CGG - chr7.3:472667-472686 None:intergenic 45.0%
GACGAAACTTCCTTGCCAAA+TGG + chr7.3:472206-472225 MS.gene071704:CDS 45.0%
GGTTCGATCAGAGGACTTAT+TGG - chr7.3:472645-472664 None:intergenic 45.0%
TACCAGAAAGAAGACGAGAC+AGG + chr7.3:472737-472756 MS.gene071704:CDS 45.0%
TATCAACAGAGTCATCCCAG+GGG - chr7.3:473246-473265 None:intergenic 45.0%
TTGCTGCTGAAGAAAGGTTC+AGG + chr7.3:472899-472918 MS.gene071704:CDS 45.0%
! ACTGGCTTATACTGAGGCAA+AGG + chr7.3:473102-473121 MS.gene071704:CDS 45.0%
! ATCGGTGAGTTGGAACTGAA+CGG - chr7.3:471910-471929 None:intergenic 45.0%
! GGATTTTGCGTGCTACAAAC+CGG + chr7.3:472067-472086 MS.gene071704:CDS 45.0%
!! GCTATTTTCCGCTCTTTCTC+CGG - chr7.3:472089-472108 None:intergenic 45.0%
!! TCGTCGGATTTTGATAACCG+CGG - chr7.3:471766-471785 None:intergenic 45.0%
!! TGTATCGGTGTCAAGTTGGA+CGG - chr7.3:471973-471992 None:intergenic 45.0%
!!! GAGACGGTGGAGTTTTAGAT+CGG - chr7.3:471928-471947 None:intergenic 45.0%
!!! GGAGTTTTAGATCGGTGAGT+TGG - chr7.3:471920-471939 None:intergenic 45.0%
AACAGAAACAGGGACGCTGA+CGG - chr7.3:473275-473294 None:intergenic 50.0%
AACCCGGTCCAAATACAAGC+TGG + chr7.3:472661-472680 MS.gene071704:CDS 50.0%
AAGACCACCGCGTAGGAATT+TGG - chr7.3:472521-472540 None:intergenic 50.0%
ACGATCCCCACCAATTTACC+TGG + chr7.3:472043-472062 MS.gene071704:CDS 50.0%
ACGGTGAGGAAGAGTAAGAG+AGG - chr7.3:471891-471910 None:intergenic 50.0%
AGTGGCATATGGAACCGTCT+CGG + chr7.3:473029-473048 MS.gene071704:CDS 50.0%
ATCCCCACCAATTTACCTGG+TGG + chr7.3:472046-472065 MS.gene071704:CDS 50.0%
CCAACTCAGTGAGTGGCATA+TGG + chr7.3:473018-473037 MS.gene071704:CDS 50.0%
CCATATGCCACTCACTGAGT+TGG - chr7.3:473021-473040 None:intergenic 50.0%
CGAGCAAGAGTTGTGAGAGA+CGG - chr7.3:471944-471963 None:intergenic 50.0%
CGGTGAGGAAGAGTAAGAGA+GGG - chr7.3:471890-471909 None:intergenic 50.0%
CTATGCAGAGTCATCCGAGA+CGG - chr7.3:473046-473065 None:intergenic 50.0%
CTTATACTGAGGCAAAGGGC+CGG + chr7.3:473107-473126 MS.gene071704:CDS 50.0%
CTTGACACCGATACAGCCAT+CGG + chr7.3:471978-471997 MS.gene071704:CDS 50.0%
GAAGACGAGACAGGGAAAGT+TGG + chr7.3:472746-472765 MS.gene071704:CDS 50.0%
GCTACAAACCGGAGAAAGAG+CGG + chr7.3:472078-472097 MS.gene071704:CDS 50.0%
GTGTAACAGTGCTGTGTGGA+AGG + chr7.3:473180-473199 MS.gene071704:CDS 50.0%
TCCCTGTCTCGTCTTCTTTC+TGG - chr7.3:472742-472761 None:intergenic 50.0%
TGTAGCACGCAAAATCCACC+AGG - chr7.3:472064-472083 None:intergenic 50.0%
TTATACTGAGGCAAAGGGCC+GGG + chr7.3:473108-473127 MS.gene071704:CDS 50.0%
! TGAGTTGGAACTGAACGGTG+AGG - chr7.3:471905-471924 None:intergenic 50.0%
!! TCGGATTTTGATAACCGCGG+AGG - chr7.3:471763-471782 None:intergenic 50.0%
!! TGGCTGTATCGGTGTCAAGT+TGG - chr7.3:471977-471996 None:intergenic 50.0%
AAAGGGCCGGGTTGCAAAAG+GGG + chr7.3:473120-473139 MS.gene071704:CDS 55.0%
ACAGAAACAGGGACGCTGAC+GGG - chr7.3:473274-473293 None:intergenic 55.0%
CAAAGGGCCGGGTTGCAAAA+GGG + chr7.3:473119-473138 MS.gene071704:CDS 55.0%
CGGGTCTGGCAGATTGCATT+CGG + chr7.3:473145-473164 MS.gene071704:CDS 55.0%
CTGGCAGATTGCATTCGGGT+CGG + chr7.3:473150-473169 MS.gene071704:CDS 55.0%
GCAAGAGTTGTGAGAGACGG+TGG - chr7.3:471941-471960 None:intergenic 55.0%
GGGTCGCAACAGCATAGACA+TGG + chr7.3:471695-471714 MS.gene071704:CDS 55.0%
GGGTCTGGCAGATTGCATTC+GGG + chr7.3:473146-473165 MS.gene071704:CDS 55.0%
TCGCGTAGTACAGACCGAAG+AGG - chr7.3:472022-472041 None:intergenic 55.0%
TGGCAGATTGCATTCGGGTC+GGG + chr7.3:473151-473170 MS.gene071704:CDS 55.0%
TTGCAAAAGGGGATCGGGTC+TGG + chr7.3:473131-473150 MS.gene071704:CDS 55.0%
TTTGGACCGGGTTCGATCAG+AGG - chr7.3:472654-472673 None:intergenic 55.0%
! CGCCGCATTTTGTCAAGAGC+AGG + chr7.3:472176-472195 MS.gene071704:CDS 55.0%
! GGAGGTATGGGTTGTAGTGC+TGG + chr7.3:472452-472471 MS.gene071704:CDS 55.0%
CCGGGTTGCAAAAGGGGATC+GGG + chr7.3:473126-473145 MS.gene071704:CDS 60.0%
GCAAAGGGCCGGGTTGCAAA+AGG + chr7.3:473118-473137 MS.gene071704:CDS 60.0%
GCCGGGTTGCAAAAGGGGAT+CGG + chr7.3:473125-473144 MS.gene071704:CDS 60.0%
GTATAGCGGCGCCACCCATT+CGG - chr7.3:472616-472635 None:intergenic 60.0%
! CCCGATCCCCTTTTGCAACC+CGG - chr7.3:473129-473148 None:intergenic 60.0%
! CTACGCGGTGGTCTTGAGCA+CGG + chr7.3:472526-472545 MS.gene071704:CDS 60.0%
ACTTGCCCGTCGTCGGGGAT+TGG + chr7.3:473212-473231 MS.gene071704:CDS 65.0%
CGTGCTCAAGACCACCGCGT+AGG - chr7.3:472528-472547 None:intergenic 65.0%
GACGACGGGCAAGTCGCGCA+CGG - chr7.3:473206-473225 None:intergenic 70.0%
GTGCGCGACTTGCCCGTCGT+CGG + chr7.3:473205-473224 MS.gene071704:CDS 70.0%
TGCCCGTCGTCGGGGATTGG+CGG + chr7.3:473215-473234 MS.gene071704:CDS 70.0%
TGCGCGACTTGCCCGTCGTC+GGG + chr7.3:473206-473225 MS.gene071704:CDS 70.0%
! GGATTGGCGGGGCAACCCCT+GGG + chr7.3:473228-473247 MS.gene071704:CDS 70.0%
CCCCGCCAATCCCCGACGAC+GGG - chr7.3:473220-473239 None:intergenic 75.0%
GCCCCGCCAATCCCCGACGA+CGG - chr7.3:473221-473240 None:intergenic 75.0%
GCGCGACTTGCCCGTCGTCG+GGG + chr7.3:473207-473226 MS.gene071704:CDS 75.0%
! CCCGTCGTCGGGGATTGGCG+GGG + chr7.3:473217-473236 MS.gene071704:CDS 75.0%
! GCCCGTCGTCGGGGATTGGC+GGG + chr7.3:473216-473235 MS.gene071704:CDS 75.0%
! GGGATTGGCGGGGCAACCCC+TGG + chr7.3:473227-473246 MS.gene071704:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 471693 473303 471693 ID=MS.gene071704
chr7.3 mRNA 471693 473303 471693 ID=MS.gene071704.t1;Parent=MS.gene071704
chr7.3 exon 471693 473303 471693 ID=MS.gene071704.t1.exon1;Parent=MS.gene071704.t1
chr7.3 CDS 471693 473303 471693 ID=cds.MS.gene071704.t1;Parent=MS.gene071704.t1
Gene Sequence

>MS.gene071704

ATGGGTCGCAACAGCATAGACATGGATAAAGAAAGACTAACGGCGGAGATGAATTTCAAAGATTCAACCTCCGCGGTTATCAAAATCCGACGACGTTTACCGGATTTTCTACAGACGGTGAAACTCAAGTACGTAAAGTTAGGTTATGGCTATTCATGCAACGCCACAACCATTCTCATTTTCACACTCGTCGTCCCTCTCTTACTCTTCCTCACCGTTCAGTTCCAACTCACCGATCTAAAACTCCACCGTCTCTCACAACTCTTGCTCGAACACACCGTCCAACTTGACACCGATACAGCCATCGGTTCAATTGTTCTACTCTTCCTCTTCGGTCTGTACTACGCGAAACGATCCCCACCAATTTACCTGGTGGATTTTGCGTGCTACAAACCGGAGAAAGAGCGGAAAATAGCAGTGGAATCTTTTGTTAAAATGTTAGAAGATAGTGGCGAATTCGGAGAAGAAACACTTCAATTTCAACGCCGCATTTTGTCAAGAGCAGGTTTAGGCGACGAAACTTCCTTGCCAAATGGAATAATGTCAAGTCCACCAAATCTATGCATGAAAGAAGCGCGTTTAGAAGCAGAAGCTGTAATGTTTGGATCATTAGACGCACTTTTTGCTAAAACAGGCGTTAATCCAAGAGACATTGACATTCTTGTTGTAAATTGTAGTTTGTTTAACCCAACTCCTTCACTTTCTGCAATGATTGTGAACCACTACAAGCTAAGAACAAACATTAAAAGCTATAATCTCGGAGGTATGGGTTGTAGTGCTGGTCTTATTTCAATTGATCTTGCCAAAGATCTTTTGAAAGCAAATCCAAATTCCTACGCGGTGGTCTTGAGCACGGAGAATTTAACCCTCAATTGGTACTTTGGAAATGATCGTTCAATGCTTCTTTCTAATTGCATTTTCCGAATGGGTGGCGCCGCTATACTTCTTTCCAATAAGTCCTCTGATCGAACCCGGTCCAAATACAAGCTGGTTCATACAGTTAGAACCCATAAAGGAGCTGATGACAAGAACTATAACTGTGTTTACCAGAAAGAAGACGAGACAGGGAAAGTTGGTGTTTCATTGGCTAGAGAATTAATGGCTGTTGCAGGAGATGCTTTGAAAACAAACATAACAACGTTGGGTCCATTGGTTTTGCCATTTTCTGAACAGTTCATGTTTTTTGTGTCTTTGGTTAAAAGGAAGTTGCTGCTGAAGAAAGGTTCAGGTGTGAAACCTTATATACCAGATTTCAAATTAGCTTTTGAACATTTCTGTATTCATGCTGGTGGAAGAGCTGTTCTTGATGAGTTGCAGAAGAATCTCCAACTCAGTGAGTGGCATATGGAACCGTCTCGGATGACTCTGCATAGATTTGGAAACACTTCAAGCAGTTCTCTATGGTATGAACTGGCTTATACTGAGGCAAAGGGCCGGGTTGCAAAAGGGGATCGGGTCTGGCAGATTGCATTCGGGTCGGGCTTTAAGTGTAACAGTGCTGTGTGGAAGGCCGTGCGCGACTTGCCCGTCGTCGGGGATTGGCGGGGCAACCCCTGGGATGACTCTGTTGATAAGTATCCCGTCAGCGTCCCTGTTTCTGTTGCTTCTTAA
Protein sequence

>MS.gene071704.t1

MGRNSIDMDKERLTAEMNFKDSTSAVIKIRRRLPDFLQTVKLKYVKLGYGYSCNATTILIFTLVVPLLLFLTVQFQLTDLKLHRLSQLLLEHTVQLDTDTAIGSIVLLFLFGLYYAKRSPPIYLVDFACYKPEKERKIAVESFVKMLEDSGEFGEETLQFQRRILSRAGLGDETSLPNGIMSSPPNLCMKEARLEAEAVMFGSLDALFAKTGVNPRDIDILVVNCSLFNPTPSLSAMIVNHYKLRTNIKSYNLGGMGCSAGLISIDLAKDLLKANPNSYAVVLSTENLTLNWYFGNDRSMLLSNCIFRMGGAAILLSNKSSDRTRSKYKLVHTVRTHKGADDKNYNCVYQKEDETGKVGVSLARELMAVAGDALKTNITTLGPLVLPFSEQFMFFVSLVKRKLLLKKGSGVKPYIPDFKLAFEHFCIHAGGRAVLDELQKNLQLSEWHMEPSRMTLHRFGNTSSSSLWYELAYTEAKGRVAKGDRVWQIAFGSGFKCNSAVWKAVRDLPVVGDWRGNPWDDSVDKYPVSVPVSVAS