Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071775.t1 | RHN51462.1 | 92.3 | 130 | 9 | 1 | 1 | 130 | 286 | 414 | 1.10E-62 | 249.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071775.t1 | A0A072U9Z1 | 92.3 | 130 | 9 | 1 | 1 | 130 | 286 | 414 | 7.6e-63 | 249.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049910 | MS.gene071775 | 0.80208 | 6.65E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071775.t1 | MTR_6g048350 | 91.603 | 131 | 10 | 1 | 1 | 131 | 286 | 415 | 4.63e-80 | 242 |
| MS.gene071775.t1 | MTR_4g094560 | 89.231 | 130 | 13 | 1 | 2 | 131 | 277 | 405 | 3.32e-78 | 237 |
| MS.gene071775.t1 | MTR_4g094570 | 84.733 | 131 | 19 | 1 | 1 | 131 | 286 | 415 | 3.05e-75 | 229 |
| MS.gene071775.t1 | MTR_4g094548 | 85.496 | 131 | 18 | 1 | 1 | 131 | 288 | 417 | 8.50e-75 | 229 |
| MS.gene071775.t1 | MTR_6g048310 | 87.023 | 131 | 12 | 2 | 1 | 131 | 284 | 409 | 1.26e-73 | 225 |
| MS.gene071775.t1 | MTR_4g094555 | 86.923 | 130 | 16 | 1 | 2 | 131 | 287 | 415 | 1.91e-73 | 225 |
| MS.gene071775.t1 | MTR_4g094562 | 86.508 | 126 | 16 | 1 | 1 | 126 | 286 | 410 | 5.38e-72 | 221 |
| MS.gene071775.t1 | MTR_4g094550 | 81.679 | 131 | 23 | 1 | 1 | 131 | 287 | 416 | 4.42e-71 | 219 |
| MS.gene071775.t1 | MTR_2g030480 | 69.767 | 129 | 38 | 1 | 1 | 129 | 261 | 388 | 4.79e-61 | 192 |
| MS.gene071775.t1 | MTR_1g052640 | 67.692 | 130 | 41 | 1 | 1 | 130 | 282 | 410 | 5.31e-60 | 190 |
| MS.gene071775.t1 | MTR_2g030470 | 70.543 | 129 | 37 | 1 | 1 | 129 | 284 | 411 | 8.31e-59 | 194 |
| MS.gene071775.t1 | MTR_2g030470 | 66.406 | 128 | 42 | 1 | 1 | 128 | 696 | 822 | 4.03e-54 | 181 |
| MS.gene071775.t1 | MTR_0083s0100 | 67.692 | 130 | 41 | 1 | 1 | 130 | 282 | 410 | 5.62e-58 | 185 |
| MS.gene071775.t1 | MTR_2g030460 | 64.122 | 131 | 46 | 1 | 1 | 131 | 282 | 411 | 2.03e-55 | 178 |
| MS.gene071775.t1 | MTR_4g094565 | 80.899 | 89 | 16 | 1 | 43 | 131 | 247 | 334 | 1.43e-42 | 143 |
| MS.gene071775.t1 | MTR_3g035040 | 54.386 | 114 | 50 | 1 | 17 | 130 | 297 | 408 | 1.62e-38 | 134 |
| MS.gene071775.t1 | MTR_8g039300 | 54.464 | 112 | 49 | 1 | 17 | 128 | 297 | 406 | 5.49e-38 | 132 |
| MS.gene071775.t1 | MTR_6g089930 | 52.632 | 114 | 52 | 1 | 17 | 130 | 297 | 408 | 9.59e-38 | 132 |
| MS.gene071775.t1 | MTR_6g090090 | 56.190 | 105 | 44 | 1 | 23 | 127 | 319 | 421 | 5.47e-36 | 127 |
| MS.gene071775.t1 | MTR_1g035220 | 61.728 | 81 | 31 | 0 | 19 | 99 | 278 | 358 | 2.62e-32 | 117 |
| MS.gene071775.t1 | MTR_2g030460 | 58.491 | 53 | 22 | 0 | 1 | 53 | 282 | 334 | 1.98e-16 | 73.9 |
| MS.gene071775.t1 | MTR_1g052640 | 60.377 | 53 | 21 | 0 | 1 | 53 | 282 | 334 | 8.77e-16 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071775.t1 | AT5G61820 | 60.156 | 128 | 46 | 3 | 1 | 128 | 297 | 419 | 6.29e-45 | 152 |
| MS.gene071775.t1 | AT5G61820 | 60.156 | 128 | 46 | 3 | 1 | 128 | 297 | 419 | 6.29e-45 | 152 |
Find 0 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTAATTAACAAGAAAA+TGG | - | 42984:5243-5262 | MS.gene071775:intron | 15.0% |
| !! | AATTTGCAATAGTTACATAT+AGG | - | 42984:4959-4978 | MS.gene071775:CDS | 20.0% |
| !! | ATTAAAACATTTATTACCCT+CGG | + | 42984:5232-5251 | None:intergenic | 20.0% |
| !!! | TTTGTTTAAAACTGTAATCA+TGG | - | 42984:5006-5025 | MS.gene071775:CDS | 20.0% |
| ! | CATTAGACCAATAGTAAATA+AGG | - | 42984:5416-5435 | MS.gene071775:intron | 25.0% |
| ! | TTAAAACTGTAATCATGGAA+GGG | - | 42984:5011-5030 | MS.gene071775:CDS | 25.0% |
| !! | AAAATCATGATTTTGTGCTA+GGG | - | 42984:5151-5170 | MS.gene071775:intron | 25.0% |
| !! | TGTGAATTATGTATGTTTTC+AGG | - | 42984:5588-5607 | MS.gene071775:intron | 25.0% |
| !! | TTTAAAACTGTAATCATGGA+AGG | - | 42984:5010-5029 | MS.gene071775:CDS | 25.0% |
| !!! | AAACTAGCCTTATTTACTAT+TGG | + | 42984:5426-5445 | None:intergenic | 25.0% |
| !!! | AATTATGTATGTTTTCAGGA+TGG | - | 42984:5592-5611 | MS.gene071775:intron | 25.0% |
| !!! | ATTATGTATGTTTTCAGGAT+GGG | - | 42984:5593-5612 | MS.gene071775:intron | 25.0% |
| !!! | TTTAAAATGAGTGTCGTTTA+AGG | + | 42984:5504-5523 | None:intergenic | 25.0% |
| TAGAAGCTAATATCTTGAGT+CGG | + | 42984:5099-5118 | None:intergenic | 30.0% | |
| TCATTTCCATTTCCATAACT+TGG | + | 42984:5640-5659 | None:intergenic | 30.0% | |
| ! | AATTAATTGGAGGGTACAAT+TGG | + | 42984:5372-5391 | None:intergenic | 30.0% |
| ! | CAAAATCATGATTTTGTGCT+AGG | - | 42984:5150-5169 | MS.gene071775:intron | 30.0% |
| ! | CTAGCACAAAATCATGATTT+TGG | + | 42984:5152-5171 | None:intergenic | 30.0% |
| !! | ATCATGATTTTGGTCTGAAT+TGG | + | 42984:5142-5161 | None:intergenic | 30.0% |
| !! | CTAATGCTTTGAAGAAAGTA+GGG | + | 42984:5402-5421 | None:intergenic | 30.0% |
| !! | TCATGATTTTGGTCTGAATT+GGG | + | 42984:5141-5160 | None:intergenic | 30.0% |
| !! | TCTAATGCTTTGAAGAAAGT+AGG | + | 42984:5403-5422 | None:intergenic | 30.0% |
| !!! | TTTTGTTTTTTTTCAGCCGA+GGG | - | 42984:5213-5232 | MS.gene071775:intron | 30.0% |
| AGAAGCTAATATCTTGAGTC+GGG | + | 42984:5098-5117 | None:intergenic | 35.0% | |
| ATGATTGTGTTCATGTGAAG+AGG | - | 42984:4844-4863 | MS.gene071775:CDS | 35.0% | |
| CTCGGCTGAAAAAAAACAAA+AGG | + | 42984:5214-5233 | None:intergenic | 35.0% | |
| GGAATTGGATCAACTCTATA+TGG | - | 42984:4930-4949 | MS.gene071775:CDS | 35.0% | |
| GGTACACTCAATTAATTGGA+GGG | + | 42984:5381-5400 | None:intergenic | 35.0% | |
| GGTTCTGTCAAGATAAATGA+TGG | - | 42984:5715-5734 | MS.gene071775:CDS | 35.0% | |
| GTAGGGTACACTCAATTAAT+TGG | + | 42984:5385-5404 | None:intergenic | 35.0% | |
| TATGGAAATGGAAATGAAGC+AGG | - | 42984:5643-5662 | MS.gene071775:intron | 35.0% | |
| TCATTTATCTTGACAGAACC+TGG | + | 42984:5715-5734 | None:intergenic | 35.0% | |
| TCTGTACCAAGTTATGGAAA+TGG | - | 42984:5631-5650 | MS.gene071775:intron | 35.0% | |
| TGTTCTTCTGTACCAAGTTA+TGG | - | 42984:5625-5644 | MS.gene071775:intron | 35.0% | |
| ! | AATGGGACTTTTCTACCTAT+TGG | - | 42984:5795-5814 | MS.gene071775:CDS | 35.0% |
| ! | ACGACACTCATTTTAAACAG+AGG | - | 42984:5507-5526 | MS.gene071775:intron | 35.0% |
| ! | CGACACTCATTTTAAACAGA+GGG | - | 42984:5508-5527 | MS.gene071775:intron | 35.0% |
| ! | GAGAGTTTTACCAATCATTG+TGG | - | 42984:5067-5086 | MS.gene071775:intron | 35.0% |
| !! | TCTACTTGTTATCCTAAACC+AGG | - | 42984:5694-5713 | MS.gene071775:CDS | 35.0% |
| !!! | CTTTTGTTTTTTTTCAGCCG+AGG | - | 42984:5212-5231 | MS.gene071775:intron | 35.0% |
| ACATAACCACCAGTTTGTAG+TGG | + | 42984:4879-4898 | None:intergenic | 40.0% | |
| ACTACAACAACACACCAAAG+AGG | - | 42984:5761-5780 | MS.gene071775:CDS | 40.0% | |
| ATCTTGACAGAACCTGGTTT+AGG | + | 42984:5709-5728 | None:intergenic | 40.0% | |
| CACAATCATTTCCCTCATCA+TGG | + | 42984:4833-4852 | None:intergenic | 40.0% | |
| CATAACCACCAGTTTGTAGT+GGG | + | 42984:4878-4897 | None:intergenic | 40.0% | |
| CATCAACATTCAGGTGGAAT+TGG | - | 42984:4915-4934 | MS.gene071775:CDS | 40.0% | |
| GGGTACACTCAATTAATTGG+AGG | + | 42984:5382-5401 | None:intergenic | 40.0% | |
| TGGAAATGAAGCAGGAAATG+AGG | - | 42984:5651-5670 | MS.gene071775:intron | 40.0% | |
| TGGATCAACTCTATATGGAC+AGG | - | 42984:4935-4954 | MS.gene071775:CDS | 40.0% | |
| TTTGTGCTAGGGTTCAAACT+TGG | - | 42984:5162-5181 | MS.gene071775:intron | 40.0% | |
| AATGAAGCAGGAAATGAGGC+AGG | - | 42984:5655-5674 | MS.gene071775:intron | 45.0% | |
| AATGAGGCAGGGTACATTGT+AGG | - | 42984:5667-5686 | MS.gene071775:intron | 45.0% | |
| ACAAGTCTCCCACTACAAAC+TGG | - | 42984:4867-4886 | MS.gene071775:CDS | 45.0% | |
| AGCTAATATCTTGAGTCGGG+TGG | + | 42984:5095-5114 | None:intergenic | 45.0% | |
| ATGAAGCAGGAAATGAGGCA+GGG | - | 42984:5656-5675 | MS.gene071775:intron | 45.0% | |
| GCTGTTGCTCATCAACATTC+AGG | - | 42984:4906-4925 | MS.gene071775:CDS | 45.0% | |
| GTTGCTCATCAACATTCAGG+TGG | - | 42984:4909-4928 | MS.gene071775:CDS | 45.0% | |
| TGTAGCACAGACCATGATGA+GGG | - | 42984:4819-4838 | MS.gene071775:CDS | 45.0% | |
| TTGTAGCACAGACCATGATG+AGG | - | 42984:4818-4837 | MS.gene071775:CDS | 45.0% | |
| ! | GGGACTTTTCTACCTATTGG+TGG | - | 42984:5798-5817 | MS.gene071775:CDS | 45.0% |
| AGTCTCCCACTACAAACTGG+TGG | - | 42984:4870-4889 | MS.gene071775:CDS | 50.0% | |
| ATTACTCCAGCGTGCCTCTT+TGG | + | 42984:5778-5797 | None:intergenic | 50.0% | |
| GGAGTTGTTCTGCCACCAAT+AGG | + | 42984:5813-5832 | None:intergenic | 50.0% | |
| TCGGGTGGAACCACAATGAT+TGG | + | 42984:5080-5099 | None:intergenic | 50.0% | |
| ! | AAAGAGGCACGCTGGAGTAA+TGG | - | 42984:5777-5796 | MS.gene071775:CDS | 50.0% |
| ! | AAGAGGCACGCTGGAGTAAT+GGG | - | 42984:5778-5797 | MS.gene071775:CDS | 50.0% |
| GCTTCTACAGTTGCGCGTAG+AGG | - | 42984:5112-5131 | MS.gene071775:intron | 55.0% | |
| ! | AACACACCAAAGAGGCACGC+TGG | - | 42984:5769-5788 | MS.gene071775:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42984 | gene | 4798 | 5849 | 4798 | ID=MS.gene071775 |
| 42984 | mRNA | 4798 | 5849 | 4798 | ID=MS.gene071775.t1;Parent=MS.gene071775 |
| 42984 | exon | 5691 | 5849 | 5691 | ID=MS.gene071775.t1.exon1;Parent=MS.gene071775.t1 |
| 42984 | CDS | 5691 | 5849 | 5691 | ID=cds.MS.gene071775.t1;Parent=MS.gene071775.t1 |
| 42984 | exon | 4798 | 5037 | 4798 | ID=MS.gene071775.t1.exon2;Parent=MS.gene071775.t1 |
| 42984 | CDS | 4798 | 5037 | 4798 | ID=cds.MS.gene071775.t1;Parent=MS.gene071775.t1 |
| Gene Sequence |
| Protein sequence |
