Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072230.t1 | AET01507.1 | 91.7 | 181 | 15 | 0 | 1 | 181 | 179 | 359 | 1.70E-89 | 339 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072230.t1 | G7LGM7 | 91.7 | 181 | 15 | 0 | 1 | 181 | 179 | 359 | 1.2e-89 | 339.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene072230 | 0.812572 | 3.96E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene072230 | 0.808977 | 2.38E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072230.t1 | MTR_8g014330 | 89.362 | 188 | 19 | 1 | 1 | 187 | 179 | 366 | 9.13e-121 | 347 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072230.t1 | AT1G79760 | 38.806 | 134 | 64 | 6 | 19 | 150 | 140 | 257 | 2.43e-18 | 81.6 |
| MS.gene072230.t1 | AT1G79760 | 38.806 | 134 | 64 | 6 | 19 | 150 | 160 | 277 | 2.50e-18 | 82.0 |
Find 37 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATTTGTTTGAAGTTTCT+TGG | 0.181857 | 8.1:-83093374 | None:intergenic |
| GAAGGTGGCTGAGAAAATTT+TGG | 0.236901 | 8.1:+83092694 | MS.gene072230:CDS |
| ATTGGAAAGGCAATATTTAA+AGG | 0.263472 | 8.1:+83093301 | MS.gene072230:CDS |
| ATTACTAGGGCACCTTGTTC+AGG | 0.285696 | 8.1:-83092980 | None:intergenic |
| GGGCATTACAAGGATGTAAT+TGG | 0.300085 | 8.1:+83093283 | MS.gene072230:CDS |
| GATCTCCAGCAGTGATTACT+AGG | 0.312300 | 8.1:-83092994 | None:intergenic |
| CAAGAAACTTCAAACAAATA+TGG | 0.338970 | 8.1:+83093375 | MS.gene072230:CDS |
| AGCATCATCAAGATATTAAT+AGG | 0.354575 | 8.1:+83092810 | MS.gene072230:CDS |
| ATGTGATTAGAATGCTGATT+AGG | 0.357036 | 8.1:+83092864 | MS.gene072230:CDS |
| GCAAGTGATGGTGATATAAT+TGG | 0.358719 | 8.1:+83092749 | MS.gene072230:CDS |
| GCTTGTTGGCCAAGTGAAAT+AGG | 0.379497 | 8.1:-83093403 | None:intergenic |
| TGTGATTAGAATGCTGATTA+GGG | 0.384993 | 8.1:+83092865 | MS.gene072230:CDS |
| ACTTGGCCAACAAGCCATCT+TGG | 0.392357 | 8.1:+83093411 | MS.gene072230:CDS |
| TTATCATATAATTGTTTAAG+TGG | 0.400439 | 8.1:-83093564 | None:intergenic |
| ATATTAATAGGAATGATCAT+TGG | 0.434233 | 8.1:+83092822 | MS.gene072230:CDS |
| AAGAGAAGACAACATTTCAA+TGG | 0.463032 | 8.1:+83093327 | MS.gene072230:CDS |
| GTTCAAAATTACAGCCAAGA+TGG | 0.463235 | 8.1:-83093425 | None:intergenic |
| TTACAGCCAAGATGGCTTGT+TGG | 0.474725 | 8.1:-83093417 | None:intergenic |
| TATTAATAGGAATGATCATT+GGG | 0.475240 | 8.1:+83092823 | MS.gene072230:CDS |
| ATTGGTCATAGAAATGAAGT+TGG | 0.490467 | 8.1:+83092767 | MS.gene072230:CDS |
| ATGTTTACTCAAAGAAAAGT+TGG | 0.492603 | 8.1:+83092945 | MS.gene072230:CDS |
| GGCTATAGGCATTGAGTGAA+GGG | 0.498659 | 8.1:+83093263 | MS.gene072230:intron |
| TTACTAGGGCACCTTGTTCA+GGG | 0.500069 | 8.1:-83092979 | None:intergenic |
| TTATGTTTGCATGTGTGTGA+TGG | 0.501762 | 8.1:+83092908 | MS.gene072230:CDS |
| TTGTCTTTCTGCCCTGAACA+AGG | 0.507702 | 8.1:+83092968 | MS.gene072230:CDS |
| TGGCTATAGGCATTGAGTGA+AGG | 0.534467 | 8.1:+83093262 | MS.gene072230:intron |
| TTGGTCATAGAAATGAAGTT+GGG | 0.535395 | 8.1:+83092768 | MS.gene072230:CDS |
| GGTGCCCTAGTAATCACTGC+TGG | 0.539493 | 8.1:+83092989 | MS.gene072230:CDS |
| CACTGCTGGAGATCAAATCC+AGG | 0.554273 | 8.1:+83093003 | MS.gene072230:CDS |
| ATTGAGTGAAGGGCATTACA+AGG | 0.566481 | 8.1:+83093273 | MS.gene072230:CDS |
| TCTACAATCACGTGTGGAGA+AGG | 0.568223 | 8.1:+83092676 | MS.gene072230:CDS |
| ATGGAAAGACCTATTTCACT+TGG | 0.570120 | 8.1:+83093394 | MS.gene072230:CDS |
| TTACAAGGATGTAATTGGAA+AGG | 0.584583 | 8.1:+83093288 | MS.gene072230:CDS |
| ATCTCCAGCAGTGATTACTA+GGG | 0.624399 | 8.1:-83092993 | None:intergenic |
| ACAATCACGTGTGGAGAAGG+TGG | 0.625775 | 8.1:+83092679 | MS.gene072230:CDS |
| GGAAACTCTACAATCACGTG+TGG | 0.665148 | 8.1:+83092670 | MS.gene072230:CDS |
| GTGATTAGAATGCTGATTAG+GGG | 0.740101 | 8.1:+83092866 | MS.gene072230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCATATAATTGTTTAAG+TGG | - | chr8.1:83093567-83093586 | None:intergenic | 15.0% |
| !! | ATATTAATAGGAATGATCAT+TGG | + | chr8.1:83092822-83092841 | MS.gene072230:CDS | 20.0% |
| !! | TATTAATAGGAATGATCATT+GGG | + | chr8.1:83092823-83092842 | MS.gene072230:CDS | 20.0% |
| ! | AAAAAGAAATTAGCAAGTGA+TGG | + | chr8.1:83092737-83092756 | MS.gene072230:CDS | 25.0% |
| ! | AGCAAAAATGTAAAATAACC+TGG | - | chr8.1:83093024-83093043 | None:intergenic | 25.0% |
| ! | AGCATCATCAAGATATTAAT+AGG | + | chr8.1:83092810-83092829 | MS.gene072230:CDS | 25.0% |
| ! | ATGTTTACTCAAAGAAAAGT+TGG | + | chr8.1:83092945-83092964 | MS.gene072230:CDS | 25.0% |
| ! | ATTGGAAAGGCAATATTTAA+AGG | + | chr8.1:83093301-83093320 | MS.gene072230:CDS | 25.0% |
| ! | CAAGAAACTTCAAACAAATA+TGG | + | chr8.1:83093375-83093394 | MS.gene072230:CDS | 25.0% |
| ! | TAAAGACAAATATTAGAACG+TGG | - | chr8.1:83093459-83093478 | None:intergenic | 25.0% |
| !! | CATATTTGTTTGAAGTTTCT+TGG | - | chr8.1:83093377-83093396 | None:intergenic | 25.0% |
| !! | TGAAGGTATAGTCAAAATAT+TGG | - | chr8.1:83093171-83093190 | None:intergenic | 25.0% |
| AAGAGAAGACAACATTTCAA+TGG | + | chr8.1:83093327-83093346 | MS.gene072230:CDS | 30.0% | |
| ATATTAGAACGTGGTACATA+AGG | - | chr8.1:83093450-83093469 | None:intergenic | 30.0% | |
| ATTGGTCATAGAAATGAAGT+TGG | + | chr8.1:83092767-83092786 | MS.gene072230:CDS | 30.0% | |
| TTAACGAGTTTGATATTGCT+TGG | + | chr8.1:83093242-83093261 | MS.gene072230:intron | 30.0% | |
| TTACAAGGATGTAATTGGAA+AGG | + | chr8.1:83093288-83093307 | MS.gene072230:CDS | 30.0% | |
| TTGGTCATAGAAATGAAGTT+GGG | + | chr8.1:83092768-83092787 | MS.gene072230:CDS | 30.0% | |
| ! | ATGTGATTAGAATGCTGATT+AGG | + | chr8.1:83092864-83092883 | MS.gene072230:CDS | 30.0% |
| ! | TGTGATTAGAATGCTGATTA+GGG | + | chr8.1:83092865-83092884 | MS.gene072230:CDS | 30.0% |
| AAAAAACTCCAACTCCACTT+TGG | + | chr8.1:83093083-83093102 | MS.gene072230:intron | 35.0% | |
| AATATTGGCACACTAGCATT+AGG | - | chr8.1:83093156-83093175 | None:intergenic | 35.0% | |
| ATCAAACTCGTTAAGTGAGA+TGG | - | chr8.1:83093236-83093255 | None:intergenic | 35.0% | |
| ATGGAAAGACCTATTTCACT+TGG | + | chr8.1:83093394-83093413 | MS.gene072230:CDS | 35.0% | |
| ATTTCAAACCAAAGTGGAGT+TGG | - | chr8.1:83093094-83093113 | None:intergenic | 35.0% | |
| GTTCAAAATTACAGCCAAGA+TGG | - | chr8.1:83093428-83093447 | None:intergenic | 35.0% | |
| GTTCGTATTTCAAACCAAAG+TGG | - | chr8.1:83093100-83093119 | None:intergenic | 35.0% | |
| GTTTGATATTGCTTGGCTAT+AGG | + | chr8.1:83093249-83093268 | MS.gene072230:intron | 35.0% | |
| TCAAACTCGTTAAGTGAGAT+GGG | - | chr8.1:83093235-83093254 | None:intergenic | 35.0% | |
| TTATGTTTGCATGTGTGTGA+TGG | + | chr8.1:83092908-83092927 | MS.gene072230:CDS | 35.0% | |
| TTGCTGTCATGAAAGATTGA+AGG | - | chr8.1:83093188-83093207 | None:intergenic | 35.0% | |
| ! | GCAAGTGATGGTGATATAAT+TGG | + | chr8.1:83092749-83092768 | MS.gene072230:CDS | 35.0% |
| ! | GTGATTAGAATGCTGATTAG+GGG | + | chr8.1:83092866-83092885 | MS.gene072230:CDS | 35.0% |
| ! | TGAAGTTTCTTGGCATTTTG+AGG | - | chr8.1:83093367-83093386 | None:intergenic | 35.0% |
| !!! | TAAGATTTTGAGGTGCAAAC+AGG | - | chr8.1:83093606-83093625 | None:intergenic | 35.0% |
| ATCTCCAGCAGTGATTACTA+GGG | - | chr8.1:83092996-83093015 | None:intergenic | 40.0% | |
| GGGCATTACAAGGATGTAAT+TGG | + | chr8.1:83093283-83093302 | MS.gene072230:CDS | 40.0% | |
| ! | GAAGGTGGCTGAGAAAATTT+TGG | + | chr8.1:83092694-83092713 | MS.gene072230:CDS | 40.0% |
| !! | ATTGAGTGAAGGGCATTACA+AGG | + | chr8.1:83093273-83093292 | MS.gene072230:CDS | 40.0% |
| !! | TGGCATTTTGAGGAGAATAG+AGG | - | chr8.1:83093357-83093376 | None:intergenic | 40.0% |
| ATTGGCACACTAGCATTAGG+AGG | - | chr8.1:83093153-83093172 | None:intergenic | 45.0% | |
| GATCTCCAGCAGTGATTACT+AGG | - | chr8.1:83092997-83093016 | None:intergenic | 45.0% | |
| GGAAACTCTACAATCACGTG+TGG | + | chr8.1:83092670-83092689 | MS.gene072230:CDS | 45.0% | |
| TCTACAATCACGTGTGGAGA+AGG | + | chr8.1:83092676-83092695 | MS.gene072230:CDS | 45.0% | |
| TTGTCTTTCTGCCCTGAACA+AGG | + | chr8.1:83092968-83092987 | MS.gene072230:CDS | 45.0% | |
| ! | ATTACTAGGGCACCTTGTTC+AGG | - | chr8.1:83092983-83093002 | None:intergenic | 45.0% |
| ! | GCTTGTTGGCCAAGTGAAAT+AGG | - | chr8.1:83093406-83093425 | None:intergenic | 45.0% |
| ! | TTACAGCCAAGATGGCTTGT+TGG | - | chr8.1:83093420-83093439 | None:intergenic | 45.0% |
| ! | TTACTAGGGCACCTTGTTCA+GGG | - | chr8.1:83092982-83093001 | None:intergenic | 45.0% |
| !! | GGCTATAGGCATTGAGTGAA+GGG | + | chr8.1:83093263-83093282 | MS.gene072230:intron | 45.0% |
| !! | TGGCTATAGGCATTGAGTGA+AGG | + | chr8.1:83093262-83093281 | MS.gene072230:intron | 45.0% |
| ACAATCACGTGTGGAGAAGG+TGG | + | chr8.1:83092679-83092698 | MS.gene072230:CDS | 50.0% | |
| ACTTGGCCAACAAGCCATCT+TGG | + | chr8.1:83093411-83093430 | MS.gene072230:CDS | 50.0% | |
| CACTGCTGGAGATCAAATCC+AGG | + | chr8.1:83093003-83093022 | MS.gene072230:CDS | 50.0% | |
| GGTGCCCTAGTAATCACTGC+TGG | + | chr8.1:83092989-83093008 | MS.gene072230:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 83092668 | 83093632 | 83092668 | ID=MS.gene072230 |
| chr8.1 | mRNA | 83092668 | 83093632 | 83092668 | ID=MS.gene072230.t1;Parent=MS.gene072230 |
| chr8.1 | exon | 83092668 | 83093024 | 83092668 | ID=MS.gene072230.t1.exon1;Parent=MS.gene072230.t1 |
| chr8.1 | CDS | 83092668 | 83093024 | 83092668 | ID=cds.MS.gene072230.t1;Parent=MS.gene072230.t1 |
| chr8.1 | exon | 83093271 | 83093435 | 83093271 | ID=MS.gene072230.t1.exon2;Parent=MS.gene072230.t1 |
| chr8.1 | CDS | 83093271 | 83093435 | 83093271 | ID=cds.MS.gene072230.t1;Parent=MS.gene072230.t1 |
| chr8.1 | exon | 83093501 | 83093632 | 83093501 | ID=MS.gene072230.t1.exon3;Parent=MS.gene072230.t1 |
| chr8.1 | CDS | 83093501 | 83093632 | 83093501 | ID=cds.MS.gene072230.t1;Parent=MS.gene072230.t1 |
| Gene Sequence |
| Protein sequence |
