AlfalfaGEDB | MS.gene072391

M. sativa cultivar XinJiangDaYe / MS.gene072391

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072391.t1	XP_003625243.2	92.9	140	4	2	1	138	185	320	2.30E-60	241.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072391.t1	Q0WVU3	45.5	101	53	1	29	127	186	286	1.1e-13	77.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072391.t1	G7KVB9	92.9	140	4	2	1	138	185	320	1.7e-60	241.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene072391.t1	TF	GARP-G2-like

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058055	MS.gene072391	0.801917	7.18E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072391.t1	MTR_7g093010	92.857	140	4	2	1	138	185	320	3.32e-87	258
MS.gene072391.t1	MTR_1g053800	55.882	136	60	0	3	138	147	282	4.79e-41	139
MS.gene072391.t1	MTR_1g053835	41.667	144	76	2	2	138	220	362	3.15e-24	96.7
MS.gene072391.t1	MTR_7g093030	47.170	106	56	0	33	138	212	317	2.31e-23	94.0
MS.gene072391.t1	MTR_1g080330	41.026	117	68	1	23	138	165	281	5.43e-21	86.3
MS.gene072391.t1	MTR_1g080330	41.026	117	68	1	23	138	165	281	1.02e-20	86.7
MS.gene072391.t1	MTR_7g098250	39.474	152	66	6	1	138	229	368	1.87e-20	86.3
MS.gene072391.t1	MTR_7g115530	41.441	111	56	2	33	138	218	324	9.89e-19	80.5
MS.gene072391.t1	MTR_7g093030	50.000	82	41	0	33	114	212	293	1.62e-18	79.7
MS.gene072391.t1	MTR_7g088070	33.333	132	76	2	15	138	32	159	1.65e-18	79.3
MS.gene072391.t1	MTR_7g088070	32.258	124	84	0	15	138	32	155	1.85e-18	79.3
MS.gene072391.t1	MTR_7g115530	41.441	111	56	2	33	138	218	324	2.10e-18	80.5
MS.gene072391.t1	MTR_2g027860	41.593	113	55	3	33	138	49	157	2.16e-18	79.7
MS.gene072391.t1	MTR_5g027440	36.667	120	61	2	33	138	25	143	7.02e-15	69.7
MS.gene072391.t1	MTR_7g115530	42.105	95	42	2	33	127	218	299	7.09e-15	69.7
MS.gene072391.t1	MTR_2g027800	37.736	106	65	1	33	138	39	143	1.63e-14	68.9
MS.gene072391.t1	MTR_1g053830	42.391	92	47	3	17	107	1	87	2.82e-14	64.3
MS.gene072391.t1	MTR_8g086410	51.667	60	29	0	35	94	113	172	3.36e-14	68.6
MS.gene072391.t1	MTR_7g115530	54.545	55	25	0	33	87	218	272	4.67e-14	67.8
MS.gene072391.t1	MTR_2g027800	48.571	70	36	0	33	102	39	108	5.07e-14	67.4
MS.gene072391.t1	MTR_8g086410	60.377	53	21	0	35	87	111	163	5.37e-14	67.8
MS.gene072391.t1	MTR_8g086410	60.377	53	21	0	35	87	113	165	5.48e-14	67.8
MS.gene072391.t1	MTR_6g444980	38.017	121	59	4	33	138	35	154	1.27e-13	66.6
MS.gene072391.t1	MTR_7g115530	55.769	52	23	0	33	84	218	269	1.35e-13	65.9
MS.gene072391.t1	MTR_6g444980	38.017	121	59	4	33	138	35	154	1.71e-13	66.2
MS.gene072391.t1	MTR_1g090670	47.143	70	37	0	33	102	44	113	2.08e-13	65.9
MS.gene072391.t1	MTR_6g032990	33.871	124	65	2	32	138	41	164	4.10e-13	65.5
MS.gene072391.t1	MTR_3g450310	53.704	54	25	0	35	88	21	74	6.50e-13	63.5
MS.gene072391.t1	MTR_7g115530	54.902	51	23	0	33	83	218	268	9.27e-13	63.9
MS.gene072391.t1	MTR_2g023580	43.590	78	40	1	10	87	36	109	1.11e-12	63.2
MS.gene072391.t1	MTR_2g084230	56.604	53	23	0	35	87	99	151	2.32e-12	63.2
MS.gene072391.t1	MTR_2g084230	56.604	53	23	0	35	87	99	151	2.44e-12	62.8
MS.gene072391.t1	MTR_0223s0040	42.500	80	34	2	35	102	20	99	2.94e-12	62.4
MS.gene072391.t1	MTR_7g069660	56.604	53	23	0	35	87	73	125	3.02e-12	62.8
MS.gene072391.t1	MTR_7g068600	36.364	121	65	4	26	138	6	122	4.35e-12	61.6
MS.gene072391.t1	MTR_2g016220	54.717	53	24	0	35	87	91	143	5.00e-12	62.0
MS.gene072391.t1	MTR_2g086450	31.343	134	59	3	32	138	44	171	5.03e-12	62.0
MS.gene072391.t1	MTR_2g016220	54.717	53	24	0	35	87	91	143	5.08e-12	62.0
MS.gene072391.t1	MTR_1g112370	46.479	71	37	1	35	105	24	93	3.53e-11	59.7
MS.gene072391.t1	MTR_2g016220	55.102	49	22	0	35	83	91	139	4.12e-11	59.7
MS.gene072391.t1	MTR_8g066310	50.943	53	26	0	35	87	175	227	5.94e-11	58.9
MS.gene072391.t1	MTR_6g071625	49.057	53	27	0	35	87	204	256	6.48e-11	58.9
MS.gene072391.t1	MTR_5g041350	46.296	54	29	0	33	86	15	68	8.74e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072391.t1	AT5G06800	44.643	112	60	1	29	138	186	297	7.98e-22	89.7
MS.gene072391.t1	AT5G06800	45.299	117	57	2	29	138	186	302	2.36e-21	88.2
MS.gene072391.t1	AT2G01060	38.710	124	66	2	21	138	3	122	1.54e-20	85.1
MS.gene072391.t1	AT5G29000	42.149	121	63	4	23	138	179	297	3.43e-19	82.4
MS.gene072391.t1	AT5G29000	42.149	121	63	4	23	138	179	297	3.43e-19	82.4
MS.gene072391.t1	AT5G29000	42.149	121	63	4	23	138	222	340	3.54e-19	82.4
MS.gene072391.t1	AT3G04450	40.708	113	61	2	31	138	236	347	6.15e-19	82.0
MS.gene072391.t1	AT3G04450	40.708	113	61	2	31	138	196	307	6.78e-19	81.6
MS.gene072391.t1	AT3G04450	40.708	113	61	2	31	138	196	307	6.78e-19	81.6
MS.gene072391.t1	AT4G28610	40.602	133	64	4	13	138	208	332	1.67e-18	80.5
MS.gene072391.t1	AT3G13040	37.241	145	75	3	5	138	213	352	8.57e-18	78.6
MS.gene072391.t1	AT3G13040	37.241	145	75	3	5	138	213	352	8.57e-18	78.6
MS.gene072391.t1	AT3G13040	37.241	145	75	3	5	138	213	352	8.57e-18	78.6
MS.gene072391.t1	AT5G06800	61.667	60	21	1	29	86	186	245	3.15e-17	75.9
MS.gene072391.t1	AT5G29000	53.846	65	29	1	23	87	222	285	1.07e-15	72.0
MS.gene072391.t1	AT3G04450	52.632	57	27	0	31	87	236	292	3.93e-15	70.5
MS.gene072391.t1	AT2G42660	60.377	53	21	0	35	87	52	104	8.22e-15	69.3
MS.gene072391.t1	AT3G12730	54.098	61	28	0	33	93	23	83	1.07e-14	68.6
MS.gene072391.t1	AT1G79430	36.937	111	64	3	33	138	34	143	6.47e-14	67.4
MS.gene072391.t1	AT4G13640	35.294	119	64	3	33	138	37	155	1.16e-13	66.6
MS.gene072391.t1	AT4G13640	35.294	119	64	3	33	138	37	155	1.29e-13	66.2
MS.gene072391.t1	AT4G04605	47.619	63	32	1	35	97	16	77	1.63e-13	65.1
MS.gene072391.t1	AT2G20400	38.393	112	58	2	33	138	231	337	1.64e-13	66.6
MS.gene072391.t1	AT3G04030	33.333	126	62	3	32	138	44	166	3.11e-13	65.9
MS.gene072391.t1	AT3G04030	33.333	126	62	3	32	138	44	166	3.14e-13	65.9
MS.gene072391.t1	AT3G24120	35.294	119	64	2	33	138	41	159	3.85e-13	65.1
MS.gene072391.t1	AT3G24120	35.294	119	64	2	33	138	41	159	4.01e-13	65.1
MS.gene072391.t1	AT2G20400	38.393	112	59	2	33	138	231	338	4.52e-13	65.1
MS.gene072391.t1	AT5G18240	32.520	123	67	2	32	138	44	166	5.42e-13	65.1
MS.gene072391.t1	AT5G18240	32.520	123	67	2	32	138	44	166	5.42e-13	65.1
MS.gene072391.t1	AT2G06020	43.662	71	33	1	23	86	69	139	5.96e-13	64.7
MS.gene072391.t1	AT5G18240	32.520	123	67	2	32	138	44	166	6.20e-13	64.7
MS.gene072391.t1	AT5G18240	32.520	123	67	2	32	138	44	166	6.20e-13	64.7
MS.gene072391.t1	AT5G18240	32.520	123	67	2	32	138	44	166	6.69e-13	64.7
MS.gene072391.t1	AT2G40260	54.717	53	24	0	35	87	84	136	9.98e-13	64.3
MS.gene072391.t1	AT4G04555	54.902	51	23	0	37	87	18	68	1.51e-12	60.1
MS.gene072391.t1	AT5G45580	51.852	54	26	0	33	86	23	76	2.10e-12	62.8
MS.gene072391.t1	AT5G45580	51.852	54	26	0	33	86	7	60	2.17e-12	62.8
MS.gene072391.t1	AT1G14600	45.455	77	38	1	16	88	2	78	2.32e-12	62.4
MS.gene072391.t1	AT2G38300	56.604	53	23	0	35	87	56	108	6.84e-12	61.6
MS.gene072391.t1	AT1G69580	50.000	54	27	0	33	86	34	87	1.33e-11	60.8
MS.gene072391.t1	AT1G69580	50.000	54	27	0	33	86	34	87	1.40e-11	60.8
MS.gene072391.t1	AT4G04580	50.943	53	26	0	35	87	16	68	1.48e-11	58.9
MS.gene072391.t1	AT2G02060	50.877	57	28	0	35	91	31	87	1.63e-11	60.1
MS.gene072391.t1	AT2G02060	50.877	57	28	0	35	91	31	87	1.63e-11	60.1
MS.gene072391.t1	AT4G37180	48.333	60	31	0	33	92	210	269	2.73e-11	60.1
MS.gene072391.t1	AT4G37180	48.333	60	31	0	33	92	217	276	3.25e-11	59.7
MS.gene072391.t1	AT1G32240	44.928	69	34	1	26	94	210	274	6.27e-11	58.9
MS.gene072391.t1	AT1G32240	44.928	69	34	1	26	94	210	274	6.31e-11	58.9
MS.gene072391.t1	AT5G16560	46.032	63	30	1	26	88	216	274	9.05e-11	58.5
MS.gene072391.t1	AT5G42630	48.148	54	28	0	35	88	107	160	9.49e-11	57.8

Find 22 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCTAACAATTCTGCTTCT+AGG	0.211425	7.4:+15914249	MS.gene072391:CDS
AAGCAGACTCCTCAACTTAA+TGG	0.254270	7.4:+15914215	MS.gene072391:CDS
AAGTATTTCGCAACAGTTAG+AGG	0.311657	7.4:+15914986	MS.gene072391:CDS
TCATGTTAAAAGCCATTTGC+AGG	0.345777	7.4:+15914491	MS.gene072391:CDS
ACGCATGCATAAAGCTTTGC+AGG	0.361361	7.4:+15914638	MS.gene072391:CDS
GTGATTCCTCAATTTGCTTA+AGG	0.361437	7.4:-15914938	None:intergenic
TCTGTTCTATGTCCTTCCTC+AGG	0.498706	7.4:-15914760	None:intergenic
TAGCAGCCTTAAGCAAATTG+AGG	0.522396	7.4:+15914932	MS.gene072391:intron
CATGTAAAAGAAGAGTAAGA+TGG	0.531937	7.4:+15914282	MS.gene072391:CDS
AATTGCAGAGGCAAAGCCTA+AGG	0.561046	7.4:+15914428	MS.gene072391:intron
ATGATAATCGTTGATCACCT+TGG	0.567335	7.4:+15914329	MS.gene072391:CDS
ATCATATCTAGTATAGCCTT+AGG	0.576046	7.4:-15914444	None:intergenic
AGAAGCAGAATTGTTAGAAG+TGG	0.580769	7.4:-15914246	None:intergenic
ATAATCGTTGATCACCTTGG+TGG	0.592004	7.4:+15914332	MS.gene072391:CDS
TTAAGCAAATTGAGGAATCA+CGG	0.605256	7.4:+15914940	MS.gene072391:CDS
GCACACATTCCATTAAGTTG+AGG	0.605770	7.4:-15914224	None:intergenic
CGCATGCATAAAGCTTTGCA+GGG	0.606639	7.4:+15914639	MS.gene072391:CDS
AAGAAGAGTAAGATGGACAG+AGG	0.623246	7.4:+15914289	MS.gene072391:CDS
ATCACGGCAGTTGCATCTAG+AGG	0.629262	7.4:+15914956	MS.gene072391:CDS
CTTTCAGAAAGACCTGAGGA+AGG	0.638784	7.4:+15914748	MS.gene072391:intron
TTATCTTTCAGAAAGACCTG+AGG	0.675030	7.4:+15914744	MS.gene072391:intron
ACTTACTTTCTGGACCACCA+AGG	0.699661	7.4:-15914346	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTATTGAAATTGTTGAA+TGG	-	chr7.4:15914528-15914547	None:intergenic	15.0%
!!	TTAGTTAATTACTTACTTTC+TGG	-	chr7.4:15914359-15914378	None:intergenic	20.0%
!!!	TTTTTTTCTCTTCTGATTAC+TGG	+	chr7.4:15914874-15914893	MS.gene072391:intron	25.0%
	ACAACACTGCTTACTTATTA+AGG	+	chr7.4:15914690-15914709	MS.gene072391:intron	30.0%
	ACAATGTTAGAGTCAATTAC+CGG	-	chr7.4:15914580-15914599	None:intergenic	30.0%
	ATCATATCTAGTATAGCCTT+AGG	-	chr7.4:15914447-15914466	None:intergenic	30.0%
	CATGTAAAAGAAGAGTAAGA+TGG	+	chr7.4:15914282-15914301	MS.gene072391:CDS	30.0%
	TTAAGCAAATTGAGGAATCA+CGG	+	chr7.4:15914940-15914959	MS.gene072391:CDS	30.0%
!	ATGGCTTTTAACATGAGAAA+TGG	-	chr7.4:15914487-15914506	None:intergenic	30.0%
!	CTTACTCTTCTTTTACATGT+TGG	-	chr7.4:15914281-15914300	None:intergenic	30.0%
!	TTCACTGATTATAATTGCAG+AGG	+	chr7.4:15914416-15914435	MS.gene072391:intron	30.0%
!!	GAAATAGTTTTACCTGCAAA+TGG	-	chr7.4:15914506-15914525	None:intergenic	30.0%
	ACACTGCTTACTTATTAAGG+AGG	+	chr7.4:15914693-15914712	MS.gene072391:intron	35.0%
	AGAAGCAGAATTGTTAGAAG+TGG	-	chr7.4:15914249-15914268	None:intergenic	35.0%
	ATGATAATCGTTGATCACCT+TGG	+	chr7.4:15914329-15914348	MS.gene072391:CDS	35.0%
	CTTCTAACAATTCTGCTTCT+AGG	+	chr7.4:15914249-15914268	MS.gene072391:CDS	35.0%
	TCATGTTAAAAGCCATTTGC+AGG	+	chr7.4:15914491-15914510	MS.gene072391:CDS	35.0%
	TTATCTTTCAGAAAGACCTG+AGG	+	chr7.4:15914744-15914763	MS.gene072391:intron	35.0%
!	GTGATTCCTCAATTTGCTTA+AGG	-	chr7.4:15914941-15914960	None:intergenic	35.0%
	AAGAAGAGTAAGATGGACAG+AGG	+	chr7.4:15914289-15914308	MS.gene072391:CDS	40.0%
	AAGCAGACTCCTCAACTTAA+TGG	+	chr7.4:15914215-15914234	MS.gene072391:CDS	40.0%
	ATAATCGTTGATCACCTTGG+TGG	+	chr7.4:15914332-15914351	MS.gene072391:CDS	40.0%
	TAGCAGCCTTAAGCAAATTG+AGG	+	chr7.4:15914932-15914951	MS.gene072391:intron	40.0%
	TGTAGATCGACATTTCTGCA+CGG	-	chr7.4:15914616-15914635	None:intergenic	40.0%
!	GCACACATTCCATTAAGTTG+AGG	-	chr7.4:15914227-15914246	None:intergenic	40.0%
!	TCAATTTGCTTAAGGCTGCT+AGG	-	chr7.4:15914933-15914952	None:intergenic	40.0%
	AATTGCAGAGGCAAAGCCTA+AGG	+	chr7.4:15914428-15914447	MS.gene072391:intron	45.0%
	ACGCATGCATAAAGCTTTGC+AGG	+	chr7.4:15914638-15914657	MS.gene072391:CDS	45.0%
	ACTTACTTTCTGGACCACCA+AGG	-	chr7.4:15914349-15914368	None:intergenic	45.0%
	CGCATGCATAAAGCTTTGCA+GGG	+	chr7.4:15914639-15914658	MS.gene072391:CDS	45.0%
	CTTTCAGAAAGACCTGAGGA+AGG	+	chr7.4:15914748-15914767	MS.gene072391:intron	45.0%
	GAGTCAATTACCGGCAAATG+AGG	-	chr7.4:15914571-15914590	None:intergenic	45.0%
	GCTTAAGGCTGCTAGGAAAT+AGG	-	chr7.4:15914926-15914945	None:intergenic	45.0%
	TCTGTTCTATGTCCTTCCTC+AGG	-	chr7.4:15914763-15914782	None:intergenic	45.0%
	TTCTGTGAAGCCTCATTTGC+CGG	+	chr7.4:15914558-15914577	MS.gene072391:intron	45.0%
	ATCACGGCAGTTGCATCTAG+AGG	+	chr7.4:15914956-15914975	MS.gene072391:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	15914191	15915007	15914191	ID=MS.gene072391
chr7.4	mRNA	15914191	15915007	15914191	ID=MS.gene072391.t1;Parent=MS.gene072391
chr7.4	exon	15914191	15914362	15914191	ID=MS.gene072391.t1.exon1;Parent=MS.gene072391.t1
chr7.4	CDS	15914191	15914362	15914191	ID=cds.MS.gene072391.t1;Parent=MS.gene072391.t1
chr7.4	exon	15914436	15914512	15914436	ID=MS.gene072391.t1.exon2;Parent=MS.gene072391.t1
chr7.4	CDS	15914436	15914512	15914436	ID=cds.MS.gene072391.t1;Parent=MS.gene072391.t1
chr7.4	exon	15914621	15914660	15914621	ID=MS.gene072391.t1.exon3;Parent=MS.gene072391.t1
chr7.4	CDS	15914621	15914660	15914621	ID=cds.MS.gene072391.t1;Parent=MS.gene072391.t1
chr7.4	exon	15914755	15914809	15914755	ID=MS.gene072391.t1.exon4;Parent=MS.gene072391.t1
chr7.4	CDS	15914755	15914809	15914755	ID=cds.MS.gene072391.t1;Parent=MS.gene072391.t1
chr7.4	exon	15914938	15915007	15914938	ID=MS.gene072391.t1.exon5;Parent=MS.gene072391.t1
chr7.4	CDS	15914938	15915007	15914938	ID=cds.MS.gene072391.t1;Parent=MS.gene072391.t1

Gene Sequence

>MS.gene072391

TCAGGCTCATCTACAAAGTCACAAAAGCAGACTCCTCAACTTAATGGAATGTGTGCCACTTCTAACAATTCTGCTTCTAGGAAAGCTCCAACATGTAAAAGAAGAGTAAGATGGACAGAGGATCTGCATGAATCATTCATGATAATCGTTGATCACCTTGGTGGTCCAGAAAGTAAGTAATTAACTAAGTAATAGCATTAGTTACACATAAGCAAATTCAATTAATTCACTGATTATAATTGCAGAGGCAAAGCCTAAGGCTATACTAGATATGATGAAATCAAATTTGCTATCCATTTCTCATGTTAAAAGCCATTTGCAGGTAAAACTATTTCCATTCAACAATTTCAATAATTTGTAAATGTTGTTCTGTGAAGCCTCATTTGCCGGTAATTGACTCTAACATTGTTCTTATATGATTGCCGTGCAGAAATGTCGATCTACAATACGCATGCATAAAGCTTTGCAGGGTAAATTAATTTTTTCATTGCATTCTATTACAACACTGCTTACTTATTAAGGAGGCTATGCGTATATTGTGACACGTTTATCTTTATCTTTCAGAAAGACCTGAGGAAGGACATAGAACAGATAGAGTCGCAGATCTTCAACTCAAAATGTAAATATTTTAGTGTTACGAATGATACTTATTGATTCTTTATGTAGTAATTGCAAGTTTTTTTTTTTTTTCTCTTCTGATTACTGGTTTTTTAAATTTATACACTTTGTTTTCCTATTTCCTAGCAGCCTTAAGCAAATTGAGGAATCACGGCAGTTGCATCTAGAGGTTCGAAAAAGTATTTCGCAACAGTTAGAG

Protein sequence

>MS.gene072391.t1

SGSSTKSQKQTPQLNGMCATSNNSASRKAPTCKRRVRWTEDLHESFMIIVDHLGGPEKAKPKAILDMMKSNLLSISHVKSHLQKCRSTIRMHKALQERPEEGHRTDRVADLQLKILKQIEESRQLHLEVRKSISQQLE