Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072398.t1 | XP_003625255.1 | 95.2 | 105 | 5 | 0 | 1 | 105 | 1 | 105 | 9.70E-43 | 182.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072398.t1 | Q01417 | 74.4 | 86 | 16 | 2 | 1 | 81 | 1 | 85 | 3.3e-23 | 109.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072398.t1 | G7KVD1 | 95.2 | 105 | 5 | 0 | 1 | 105 | 1 | 105 | 7.0e-43 | 182.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05316 | MS.gene072398 | 0.809524 | 1.81E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072398.t1 | MTR_7g093140 | 95.238 | 105 | 5 | 0 | 1 | 105 | 1 | 105 | 6.81e-58 | 174 |
| MS.gene072398.t1 | MTR_7g093160 | 93.069 | 101 | 7 | 0 | 1 | 101 | 1 | 101 | 7.90e-52 | 158 |
| MS.gene072398.t1 | MTR_7g093170 | 83.784 | 74 | 12 | 0 | 4 | 77 | 3 | 76 | 4.31e-36 | 121 |
| MS.gene072398.t1 | MTR_1g053970 | 68.750 | 80 | 22 | 1 | 5 | 81 | 4 | 83 | 5.85e-31 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072398.t1 | AT5G06760 | 65.672 | 67 | 23 | 0 | 10 | 76 | 2 | 68 | 4.67e-25 | 92.4 |
Find 0 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACAAATTTTAATTTAACA+GGG | - | 51337:67202-67221 | MS.gene072398:intron | 10.0% |
| !!! | AAATGTGATTTATTTTTATA+AGG | + | 51337:66716-66735 | None:intergenic | 10.0% |
| !!! | AAATTTTCATTAATTTTGTA+GGG | + | 51337:66851-66870 | None:intergenic | 10.0% |
| !!! | ATAATTTGAATTTTTTTTGT+AGG | + | 51337:67031-67050 | None:intergenic | 10.0% |
| !!! | CTTTCATTTTATATAAATAA+GGG | - | 51337:66642-66661 | MS.gene072398:CDS | 10.0% |
| !!! | TAAACAAATTTTAATTTAAC+AGG | - | 51337:67201-67220 | MS.gene072398:intron | 10.0% |
| !!! | TAATTTGAATTTTTTTTGTA+GGG | + | 51337:67030-67049 | None:intergenic | 10.0% |
| !! | CTACAAAATTAATGAAAATT+TGG | - | 51337:66850-66869 | MS.gene072398:intron | 15.0% |
| !! | TTTCAAAAATTTAGAAGTTT+AGG | - | 51337:67172-67191 | MS.gene072398:intron | 15.0% |
| !!! | CAAATTTTCATTAATTTTGT+AGG | + | 51337:66852-66871 | None:intergenic | 15.0% |
| !!! | CCTTATTTATATAAAATGAA+AGG | + | 51337:66644-66663 | None:intergenic | 15.0% |
| !!! | CCTTTCATTTTATATAAATA+AGG | - | 51337:66641-66660 | MS.gene072398:CDS | 15.0% |
| !!! | TAGTTAACATTTTTTTTTGT+TGG | - | 51337:67523-67542 | MS.gene072398:intron | 15.0% |
| !!! | TATGTTTTATCTTGAAATTT+TGG | - | 51337:66884-66903 | MS.gene072398:intron | 15.0% |
| !! | ACAAAACTGCAAATATTTAT+AGG | - | 51337:67292-67311 | MS.gene072398:intron | 20.0% |
| !! | ATTATTTACCAAAAAGATTG+CGG | + | 51337:67350-67369 | None:intergenic | 20.0% |
| !! | CAAAACTGCAAATATTTATA+GGG | - | 51337:67293-67312 | MS.gene072398:intron | 20.0% |
| !!! | ATAAGGGTTAATTAAGTTTT+TGG | - | 51337:66658-66677 | MS.gene072398:CDS | 20.0% |
| !!! | TATAGGGTTAATTAAGTTTT+TGG | + | 51337:67320-67339 | None:intergenic | 20.0% |
| ! | AAGATTGCGGAATTATTTAT+AGG | + | 51337:67337-67356 | None:intergenic | 25.0% |
| ! | ACAAAACTGCAGATATTTAT+AGG | + | 51337:66686-66705 | None:intergenic | 25.0% |
| ! | AGATTGCGGAATTATTTATA+GGG | + | 51337:67336-67355 | None:intergenic | 25.0% |
| ! | CAAAACTGCAGATATTTATA+GGG | + | 51337:66685-66704 | None:intergenic | 25.0% |
| !! | AAATAATTCCGCAATCTTTT+TGG | - | 51337:67339-67358 | MS.gene072398:intron | 25.0% |
| !! | GTTTTGTCACTAAAATTATG+AGG | + | 51337:67448-67467 | None:intergenic | 25.0% |
| AACCCAAAAGAAAGAAGAAA+GGG | - | 51337:67856-67875 | MS.gene072398:intron | 30.0% | |
| ACAGAGACTAAAAACACTAA+CGG | + | 51337:66759-66778 | None:intergenic | 30.0% | |
| TATATGCACACGTTTCAAAT+TGG | - | 51337:67707-67726 | MS.gene072398:intron | 30.0% | |
| ! | AACCCTTTCTTCTTTCTTTT+GGG | + | 51337:67861-67880 | None:intergenic | 30.0% |
| ! | ACACTAACGGAAAATTTTGT+AGG | - | 51337:67232-67251 | MS.gene072398:intron | 30.0% |
| ! | ACACTAACGGAAAATTTTGT+AGG | + | 51337:67251-67232 | None:intergenic | 30.0% |
| ! | CACTAACGGAAAATTTTGTA+GGG | - | 51337:67233-67252 | MS.gene072398:intron | 30.0% |
| ! | CACTAACGGAAAATTTTGTA+GGG | + | 51337:67252-67233 | None:intergenic | 30.0% |
| ! | TAACCCTTTCTTCTTTCTTT+TGG | + | 51337:67862-67881 | None:intergenic | 30.0% |
| AAAAACAGGAGAGTCCATTA+AGG | - | 51337:66541-66560 | MS.gene072398:CDS | 35.0% | |
| AATTACCTTCTCTTGAACAG+TGG | + | 51337:66621-66640 | None:intergenic | 35.0% | |
| ACAGGGACTAAAAACACTAA+CGG | - | 51337:67219-67238 | MS.gene072398:intron | 35.0% | |
| CAACCCAAAAGAAAGAAGAA+AGG | - | 51337:67855-67874 | MS.gene072398:intron | 35.0% | |
| CACGAGGCTAATTATTATTG+AGG | - | 51337:67476-67495 | MS.gene072398:intron | 35.0% | |
| ATCACATGATCCCTTGCAAA+AGG | - | 51337:67826-67845 | MS.gene072398:intron | 40.0% | |
| GAAAGAAGAAAGGGTTAACC+AGG | - | 51337:67865-67884 | MS.gene072398:intron | 40.0% | |
| TCAACAACTCTTCACTCATG+TGG | - | 51337:67499-67518 | MS.gene072398:intron | 40.0% | |
| ATCTGGCATGGAAAAGACCA+AGG | - | 51337:66595-66614 | MS.gene072398:CDS | 45.0% | |
| CAAGGAGGAGCGAAAAAAAC+AGG | - | 51337:66527-66546 | MS.gene072398:CDS | 45.0% | |
| CTCAAACGAGGAGTCAGTTT+GGG | - | 51337:67990-68009 | MS.gene072398:CDS | 45.0% | |
| GACAAAACTCTTTGCTCACG+AGG | - | 51337:67460-67479 | MS.gene072398:intron | 45.0% | |
| TGATCCCTTGCAAAAGGAGA+TGG | - | 51337:67832-67851 | MS.gene072398:intron | 45.0% | |
| ! | ATTGGTGCTTCAGCCAAATC+TGG | - | 51337:66578-66597 | MS.gene072398:CDS | 45.0% |
| ! | GGTCTTTTCCATGCCAGATT+TGG | + | 51337:66594-66613 | None:intergenic | 45.0% |
| ! | GTTGCCATCTCCTTTTGCAA+GGG | + | 51337:67839-67858 | None:intergenic | 45.0% |
| ! | TTTGGCTGAAGCACCAATGT+TGG | + | 51337:66576-66595 | None:intergenic | 45.0% |
| AAGGAGACAGCTGCCAACAT+TGG | - | 51337:66560-66579 | MS.gene072398:CDS | 50.0% | |
| CAAGGCCACTGTTCAAGAGA+AGG | - | 51337:66613-66632 | MS.gene072398:CDS | 50.0% | |
| CCAGGCTGAGCTTGATAAAG+AGG | - | 51337:67883-67902 | MS.gene072398:intron | 50.0% | |
| CCTCTTTATCAAGCTCAGCC+TGG | + | 51337:67886-67905 | None:intergenic | 50.0% | |
| CTTCTCTTGAACAGTGGCCT+TGG | + | 51337:66615-66634 | None:intergenic | 50.0% | |
| GCTCAAACGAGGAGTCAGTT+TGG | - | 51337:67989-68008 | MS.gene072398:CDS | 50.0% | |
| GGCTGAGCTTGATAAAGAGG+CGG | - | 51337:67886-67905 | MS.gene072398:intron | 50.0% | |
| GTTGGCAGCTGTCTCCTTAA+TGG | + | 51337:66558-66577 | None:intergenic | 50.0% | |
| TGCTTCAGCCAAATCTGGCA+TGG | - | 51337:66583-66602 | MS.gene072398:CDS | 50.0% | |
| ! | GGTTGCCATCTCCTTTTGCA+AGG | + | 51337:67840-67859 | None:intergenic | 50.0% |
| ACCACACCACGGGAACTGGT+GGG | - | 51337:67963-67982 | MS.gene072398:CDS | 60.0% | |
| ACAACGCTGCAGCCACCACC+GGG | - | 51337:67918-67937 | MS.gene072398:CDS | 65.0% | |
| ACCGGGCACCAATTGGGGCA+GGG | - | 51337:67935-67954 | MS.gene072398:CDS | 65.0% | |
| AGCCACCACCGGGCACCAAT+TGG | - | 51337:67928-67947 | MS.gene072398:CDS | 65.0% | |
| CACAACGCTGCAGCCACCAC+CGG | - | 51337:67917-67936 | MS.gene072398:CDS | 65.0% | |
| CACCACACCACGGGAACTGG+TGG | - | 51337:67962-67981 | MS.gene072398:CDS | 65.0% | |
| CAGGGTGGACACCACACCAC+GGG | - | 51337:67953-67972 | MS.gene072398:CDS | 65.0% | |
| CCACACCACGGGAACTGGTG+GGG | - | 51337:67964-67983 | MS.gene072398:CDS | 65.0% | |
| CCACCACCGGGCACCAATTG+GGG | - | 51337:67930-67949 | MS.gene072398:CDS | 65.0% | |
| CTGGTGGGGCTGCTCAAACG+AGG | - | 51337:67978-67997 | MS.gene072398:CDS | 65.0% | |
| GCAGGGTGGACACCACACCA+CGG | - | 51337:67952-67971 | MS.gene072398:CDS | 65.0% | |
| GCCACCACCGGGCACCAATT+GGG | - | 51337:67929-67948 | MS.gene072398:CDS | 65.0% | |
| GGACACCACACCACGGGAAC+TGG | - | 51337:67959-67978 | MS.gene072398:CDS | 65.0% | |
| GGTGTCCACCCTGCCCCAAT+TGG | + | 51337:67946-67965 | None:intergenic | 65.0% | |
| ! | ACCCTGCCCCAATTGGTGCC+CGG | + | 51337:67939-67958 | None:intergenic | 65.0% |
| AGCAGCCCCACCAGTTCCCG+TGG | + | 51337:67972-67991 | None:intergenic | 70.0% | |
| CACCGGGCACCAATTGGGGC+AGG | - | 51337:67934-67953 | MS.gene072398:CDS | 70.0% | |
| CCCCACCAGTTCCCGTGGTG+TGG | + | 51337:67967-67986 | None:intergenic | 70.0% | |
| GGGCACCAATTGGGGCAGGG+TGG | - | 51337:67938-67957 | MS.gene072398:CDS | 70.0% | |
| ! | CCCCAATTGGTGCCCGGTGG+TGG | + | 51337:67933-67952 | None:intergenic | 70.0% |
| ! | CTGCCCCAATTGGTGCCCGG+TGG | + | 51337:67936-67955 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51337 | gene | 66524 | 68018 | 66524 | ID=MS.gene072398 |
| 51337 | mRNA | 66524 | 68018 | 66524 | ID=MS.gene072398.t1;Parent=MS.gene072398 |
| 51337 | exon | 67908 | 68018 | 67908 | ID=MS.gene072398.t1.exon1;Parent=MS.gene072398.t1 |
| 51337 | CDS | 67908 | 68018 | 67908 | ID=cds.MS.gene072398.t1;Parent=MS.gene072398.t1 |
| 51337 | exon | 66524 | 66730 | 66524 | ID=MS.gene072398.t1.exon2;Parent=MS.gene072398.t1 |
| 51337 | CDS | 66524 | 66730 | 66524 | ID=cds.MS.gene072398.t1;Parent=MS.gene072398.t1 |
| Gene Sequence |
| Protein sequence |
