Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072459.t1 | PNY10175.1 | 96.2 | 80 | 3 | 0 | 20 | 99 | 129 | 208 | 1.60E-34 | 155.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072459.t1 | P46086 | 71.2 | 80 | 21 | 1 | 20 | 99 | 129 | 206 | 1.8e-23 | 109.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072459.t1 | A0A2K3P4F9 | 96.2 | 80 | 3 | 0 | 20 | 99 | 129 | 208 | 1.1e-34 | 155.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene072459 | 0.823666 | 1.22E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene072459 | 0.804291 | 2.32E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene072459 | 0.826508 | 2.61E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072459.t1 | MTR_7g113660 | 95.062 | 81 | 4 | 0 | 19 | 99 | 18 | 98 | 5.89e-41 | 136 |
| MS.gene072459.t1 | MTR_7g113660 | 95.000 | 80 | 4 | 0 | 20 | 99 | 128 | 207 | 5.78e-39 | 133 |
| MS.gene072459.t1 | MTR_2g026860 | 64.103 | 39 | 14 | 0 | 54 | 92 | 42 | 80 | 2.70e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072459.t1 | AT5G27450 | 71.250 | 80 | 21 | 1 | 20 | 99 | 129 | 206 | 7.28e-32 | 114 |
| MS.gene072459.t1 | AT5G27450 | 71.250 | 80 | 21 | 1 | 20 | 99 | 129 | 206 | 7.28e-32 | 114 |
| MS.gene072459.t1 | AT5G27450 | 71.250 | 80 | 21 | 1 | 20 | 99 | 129 | 206 | 7.28e-32 | 114 |
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTGATTTGGTAAATAAA+TGG | 0.186049 | 7.3:-3357829 | MS.gene072459:CDS |
| CTTCCTTTGGGATCGGGTTT+GGG | 0.252521 | 7.3:-3357959 | MS.gene072459:CDS |
| TCTTCCTTTGGGATCGGGTT+TGG | 0.254236 | 7.3:-3357960 | MS.gene072459:CDS |
| TATTAATTCTGATCTTCCTT+TGG | 0.266647 | 7.3:-3357972 | MS.gene072459:CDS |
| CAACAAGGGTGGCATTCTTT+TGG | 0.277254 | 7.3:-3357860 | MS.gene072459:CDS |
| ATTAATTCTGATCTTCCTTT+GGG | 0.302065 | 7.3:-3357971 | MS.gene072459:CDS |
| TGGAGAGAAGGAACTTGATT+TGG | 0.330091 | 7.3:-3357840 | MS.gene072459:CDS |
| TCTGATCTTCCTTTGGGATC+GGG | 0.381978 | 7.3:-3357965 | MS.gene072459:CDS |
| TTCTGATCTTCCTTTGGGAT+CGG | 0.387010 | 7.3:-3357966 | MS.gene072459:CDS |
| ACTTGATTTGGTAAATAAAT+GGG | 0.390031 | 7.3:-3357828 | MS.gene072459:CDS |
| CGCGGCATTCTGTGTGGCAC+TGG | 0.408652 | 7.3:-3357930 | MS.gene072459:CDS |
| ACTAACTGTGTTGTCAATTC+CGG | 0.449491 | 7.3:+3357769 | None:intergenic |
| ATCATTCATGGAAAGCCATC+CGG | 0.472952 | 7.3:-3357788 | MS.gene072459:CDS |
| GAATTAATAACCACAATAGC+AGG | 0.540525 | 7.3:+3357986 | None:intergenic |
| TAGATTCAAGCCTGCTATTG+TGG | 0.551090 | 7.3:-3357996 | MS.gene072459:CDS |
| AGCAACAGAATCAAAACATA+CGG | 0.559069 | 7.3:+3358028 | None:intergenic |
| TGTTACTGATTCTGTTTCTG+TGG | 0.559785 | 7.3:-3357891 | MS.gene072459:CDS |
| ATCGGGTTTGGGTTCATCCG+CGG | 0.562556 | 7.3:-3357948 | MS.gene072459:CDS |
| GGCATTCTGTGTGGCACTGG+CGG | 0.602276 | 7.3:-3357927 | MS.gene072459:CDS |
| CTGTGGATGTGATGCAACAA+GGG | 0.621460 | 7.3:-3357874 | MS.gene072459:CDS |
| TCTGTGGATGTGATGCAACA+AGG | 0.639120 | 7.3:-3357875 | MS.gene072459:CDS |
| TTCATCCGCGGCATTCTGTG+TGG | 0.639325 | 7.3:-3357936 | MS.gene072459:CDS |
| ACTGTGTTGTCAATTCCGGA+TGG | 0.645767 | 7.3:+3357773 | None:intergenic |
| GAACCCAAACCCGATCCCAA+AGG | 0.654625 | 7.3:+3357956 | None:intergenic |
| CAGTGCCACACAGAATGCCG+CGG | 0.676093 | 7.3:+3357931 | None:intergenic |
| TGGATGTGATGCAACAAGGG+TGG | 0.717984 | 7.3:-3357871 | MS.gene072459:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTTGATTTGGTAAATAAA+TGG | - | chr7.3:3357995-3358014 | MS.gene072459:CDS | 20.0% |
| !!! | ACTTGATTTGGTAAATAAAT+GGG | - | chr7.3:3357996-3358015 | MS.gene072459:CDS | 20.0% |
| ! | ATTAATTCTGATCTTCCTTT+GGG | - | chr7.3:3357853-3357872 | MS.gene072459:CDS | 25.0% |
| ! | TATTAATTCTGATCTTCCTT+TGG | - | chr7.3:3357852-3357871 | MS.gene072459:CDS | 25.0% |
| AGCAACAGAATCAAAACATA+CGG | + | chr7.3:3357799-3357818 | None:intergenic | 30.0% | |
| GAATTAATAACCACAATAGC+AGG | + | chr7.3:3357841-3357860 | None:intergenic | 30.0% | |
| !! | GTAAATAAATGGGCTTTTGA+GGG | - | chr7.3:3358006-3358025 | MS.gene072459:CDS | 30.0% |
| GAGGGTGAAAAAATCATTCA+TGG | - | chr7.3:3358024-3358043 | MS.gene072459:CDS | 35.0% | |
| !! | GGTAAATAAATGGGCTTTTG+AGG | - | chr7.3:3358005-3358024 | MS.gene072459:CDS | 35.0% |
| !! | TGTTACTGATTCTGTTTCTG+TGG | - | chr7.3:3357933-3357952 | MS.gene072459:CDS | 35.0% |
| ATCATTCATGGAAAGCCATC+CGG | - | chr7.3:3358036-3358055 | MS.gene072459:CDS | 40.0% | |
| TAGATTCAAGCCTGCTATTG+TGG | - | chr7.3:3357828-3357847 | MS.gene072459:CDS | 40.0% | |
| TGGAGAGAAGGAACTTGATT+TGG | - | chr7.3:3357984-3358003 | MS.gene072459:CDS | 40.0% | |
| !! | TTCTGATCTTCCTTTGGGAT+CGG | - | chr7.3:3357858-3357877 | MS.gene072459:CDS | 40.0% |
| CTGTGGATGTGATGCAACAA+GGG | - | chr7.3:3357950-3357969 | MS.gene072459:CDS | 45.0% | |
| TCTGTGGATGTGATGCAACA+AGG | - | chr7.3:3357949-3357968 | MS.gene072459:CDS | 45.0% | |
| ! | CAACAAGGGTGGCATTCTTT+TGG | - | chr7.3:3357964-3357983 | MS.gene072459:CDS | 45.0% |
| !! | GTGGCATTCTTTTGGAGAGA+AGG | - | chr7.3:3357972-3357991 | MS.gene072459:CDS | 45.0% |
| !! | TCTGATCTTCCTTTGGGATC+GGG | - | chr7.3:3357859-3357878 | MS.gene072459:CDS | 45.0% |
| TGGATGTGATGCAACAAGGG+TGG | - | chr7.3:3357953-3357972 | MS.gene072459:CDS | 50.0% | |
| !! | CTTCCTTTGGGATCGGGTTT+GGG | - | chr7.3:3357865-3357884 | MS.gene072459:CDS | 50.0% |
| !! | TCTTCCTTTGGGATCGGGTT+TGG | - | chr7.3:3357864-3357883 | MS.gene072459:CDS | 50.0% |
| GAACCCAAACCCGATCCCAA+AGG | + | chr7.3:3357871-3357890 | None:intergenic | 55.0% | |
| TTCATCCGCGGCATTCTGTG+TGG | - | chr7.3:3357888-3357907 | MS.gene072459:CDS | 55.0% | |
| ! | ATCGGGTTTGGGTTCATCCG+CGG | - | chr7.3:3357876-3357895 | MS.gene072459:CDS | 55.0% |
| CAGTGCCACACAGAATGCCG+CGG | + | chr7.3:3357896-3357915 | None:intergenic | 60.0% | |
| ! | GGCATTCTGTGTGGCACTGG+CGG | - | chr7.3:3357897-3357916 | MS.gene072459:CDS | 60.0% |
| CGCGGCATTCTGTGTGGCAC+TGG | - | chr7.3:3357894-3357913 | MS.gene072459:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 3357775 | 3358071 | 3357775 | ID=MS.gene072459 |
| chr7.3 | mRNA | 3357775 | 3358071 | 3357775 | ID=MS.gene072459.t1;Parent=MS.gene072459 |
| chr7.3 | exon | 3357775 | 3358071 | 3357775 | ID=MS.gene072459.t1.exon1;Parent=MS.gene072459.t1 |
| chr7.3 | CDS | 3357775 | 3358071 | 3357775 | ID=cds.MS.gene072459.t1;Parent=MS.gene072459.t1 |
| Gene Sequence |
| Protein sequence |
