Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07271.t1 | XP_003591424.1 | 87 | 1352 | 168 | 5 | 1 | 1350 | 1 | 1346 | 0.00E+00 | 2259.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07271.t1 | O04251 | 32.3 | 1379 | 632 | 30 | 9 | 1149 | 10 | 1324 | 4.6e-154 | 547.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07271.t1 | G7IDS4 | 87.0 | 1352 | 168 | 5 | 1 | 1350 | 1 | 1346 | 0.0e+00 | 2259.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07271.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04985 | MS.gene07271 | 0.82762 | 1.42E-54 | -1.69E-46 |
| MS.gene049989 | MS.gene07271 | 0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene050297 | MS.gene07271 | 0.823575 | 1.29E-53 | -1.69E-46 |
| MS.gene050648 | MS.gene07271 | 0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene054643 | MS.gene07271 | 0.812777 | 3.57E-51 | -1.69E-46 |
| MS.gene056169 | MS.gene07271 | 0.803416 | 3.52E-49 | -1.69E-46 |
| MS.gene059300 | MS.gene07271 | 0.810905 | 9.13E-51 | -1.69E-46 |
| MS.gene059778 | MS.gene07271 | 0.818057 | 2.39E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07271.t1 | MTR_1g087290 | 92.530 | 1352 | 93 | 5 | 1 | 1350 | 1 | 1346 | 0.0 | 2529 |
| MS.gene07271.t1 | MTR_1g087290 | 93.114 | 1249 | 78 | 5 | 1 | 1247 | 1 | 1243 | 0.0 | 2340 |
| MS.gene07271.t1 | MTR_1g087290 | 82.075 | 106 | 17 | 1 | 1247 | 1350 | 1138 | 1243 | 4.87e-49 | 191 |
| MS.gene07271.t1 | MTR_6g016895 | 37.500 | 96 | 57 | 3 | 111 | 205 | 4 | 97 | 2.95e-14 | 77.4 |
| MS.gene07271.t1 | MTR_6g016895 | 37.500 | 96 | 57 | 3 | 111 | 205 | 4 | 97 | 3.54e-14 | 77.0 |
| MS.gene07271.t1 | MTR_1g029630 | 26.316 | 190 | 126 | 7 | 7 | 191 | 554 | 734 | 4.78e-11 | 68.2 |
| MS.gene07271.t1 | MTR_1g029630 | 26.316 | 190 | 126 | 7 | 7 | 191 | 564 | 744 | 5.50e-11 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07271.t1 | AT4G02110 | 70.870 | 230 | 66 | 1 | 920 | 1149 | 967 | 1195 | 5.83e-114 | 387 |
| MS.gene07271.t1 | AT4G02110 | 67.290 | 107 | 30 | 1 | 129 | 235 | 1 | 102 | 1.35e-37 | 154 |
| MS.gene07271.t1 | AT4G02110 | 70.870 | 230 | 66 | 1 | 920 | 1149 | 1096 | 1324 | 1.14e-112 | 386 |
| MS.gene07271.t1 | AT4G02110 | 63.877 | 227 | 77 | 1 | 9 | 235 | 10 | 231 | 1.11e-89 | 319 |
| MS.gene07271.t1 | AT1G67180 | 36.792 | 106 | 57 | 4 | 109 | 206 | 2 | 105 | 1.36e-13 | 75.1 |
| MS.gene07271.t1 | AT1G67180 | 36.792 | 106 | 57 | 4 | 109 | 206 | 2 | 105 | 5.96e-13 | 73.2 |
| MS.gene07271.t1 | AT1G67180 | 36.792 | 106 | 57 | 4 | 109 | 206 | 2 | 105 | 5.96e-13 | 73.2 |
Find 310 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGAGTCTTTACCCTTAT+TGG | 0.075467 | 1.4:+69565230 | None:intergenic |
| GTTTGGGTGCTACTGCATTT+TGG | 0.133192 | 1.4:+69561967 | None:intergenic |
| GATTTCCTGGTCTTGATAAT+TGG | 0.143495 | 1.4:-69564210 | MS.gene07271:CDS |
| CTTTGCAGCTGTGGTCTTCT+TGG | 0.147073 | 1.4:+69563616 | None:intergenic |
| ACTTGTGGACCAGTAGAATT+TGG | 0.162831 | 1.4:+69565264 | None:intergenic |
| TCCATCTATATCAGAATTTA+AGG | 0.164801 | 1.4:-69563306 | MS.gene07271:CDS |
| AATTTCTCTGTCCTCCTTAT+AGG | 0.170617 | 1.4:+69563131 | None:intergenic |
| AAAATAAAACATGTGGTTTC+AGG | 0.179171 | 1.4:+69563272 | None:intergenic |
| AAATCGGAAAAGGCAGTATT+TGG | 0.201132 | 1.4:-69563584 | MS.gene07271:CDS |
| TATACTCGCTTTCTTGATAC+TGG | 0.214753 | 1.4:-69562402 | MS.gene07271:CDS |
| ATTTGTTAACATCAAGGATT+TGG | 0.217175 | 1.4:+69563966 | None:intergenic |
| AAAAGTCAATAAAAGTAAAA+AGG | 0.225243 | 1.4:-69561459 | MS.gene07271:CDS |
| ACAGGTCATGGTGCCTTCTA+TGG | 0.228805 | 1.4:-69562825 | MS.gene07271:CDS |
| AAAACCCAATAAAAGGAAAA+AGG | 0.231177 | 1.4:-69561929 | MS.gene07271:CDS |
| TCTTGTGTAACTTAACTTCT+TGG | 0.243171 | 1.4:+69564954 | None:intergenic |
| GCCTCATCTCTTGTCTCATT+TGG | 0.250185 | 1.4:+69563551 | None:intergenic |
| TCATTCTTTGAATTCTTTCC+TGG | 0.251009 | 1.4:+69564235 | None:intergenic |
| TTCAACAGCCGCCACTTTCT+TGG | 0.251038 | 1.4:+69562855 | None:intergenic |
| GGTGTTCATTTCGCTCTCTT+TGG | 0.264409 | 1.4:-69570239 | MS.gene07271:CDS |
| AGTCCCCTAGGTTCAAAATT+TGG | 0.268181 | 1.4:-69565345 | MS.gene07271:CDS |
| TAAAGATTTGGATGGAATTC+CGG | 0.270747 | 1.4:-69567451 | MS.gene07271:CDS |
| TCCAAAGGCACCTGGCTTCC+AGG | 0.273647 | 1.4:-69565046 | MS.gene07271:CDS |
| CAGACAGTGACATTTCTTTC+AGG | 0.274545 | 1.4:+69563516 | None:intergenic |
| TCCTTAGACTTGATATTTGC+TGG | 0.278398 | 1.4:+69563341 | None:intergenic |
| ATTCGATTCAAGCTTGTTAA+TGG | 0.292549 | 1.4:-69570094 | MS.gene07271:CDS |
| TTCTGCCTTACTTTGATGTT+TGG | 0.298597 | 1.4:+69561712 | None:intergenic |
| TGCTATTTCTTTGCTTCTGC+TGG | 0.302908 | 1.4:+69564021 | None:intergenic |
| GTACAGACCGCTTAAAGATT+TGG | 0.314245 | 1.4:-69567463 | MS.gene07271:CDS |
| CCTTGATGTTAACAAATTGA+TGG | 0.316106 | 1.4:-69563960 | MS.gene07271:CDS |
| TATTCTAGGAAGAACCAAAA+AGG | 0.316958 | 1.4:-69564871 | MS.gene07271:CDS |
| TTTGTTAACATCAAGGATTT+GGG | 0.318611 | 1.4:+69563967 | None:intergenic |
| GATTCCAAGCTGGGGCAATC+AGG | 0.318812 | 1.4:-69565387 | MS.gene07271:CDS |
| CCAGGATCGGGATGACATTA+TGG | 0.318910 | 1.4:-69567388 | MS.gene07271:intron |
| AAAGATTTGGATGGAATTCC+GGG | 0.320376 | 1.4:-69567450 | MS.gene07271:CDS |
| CTCTTGTTATATTTGTCTTC+AGG | 0.325234 | 1.4:+69566122 | None:intergenic |
| TTCCAGGATATTGACATTAC+TGG | 0.328872 | 1.4:-69565030 | MS.gene07271:CDS |
| TCTGTCCTCCTTATAGGATC+TGG | 0.341349 | 1.4:+69563137 | None:intergenic |
| TAATCTGCGTGATGACAATT+TGG | 0.341472 | 1.4:-69564980 | MS.gene07271:CDS |
| GTATCAAGAAAGCGAGTATA+AGG | 0.343349 | 1.4:+69562405 | None:intergenic |
| TCTTTACCCTTATTGGCATT+TGG | 0.346613 | 1.4:+69565237 | None:intergenic |
| GAGGATTGCAGCAATCGATA+TGG | 0.347128 | 1.4:+69561549 | None:intergenic |
| GCCAGCAAATATCAAGTCTA+AGG | 0.349651 | 1.4:-69563342 | MS.gene07271:CDS |
| TCCTGGAAGCCAGGTGCCTT+TGG | 0.349876 | 1.4:+69565045 | None:intergenic |
| GCCTTAAATTCTGATATAGA+TGG | 0.350253 | 1.4:+69563305 | None:intergenic |
| TCATAGCTCCAGATCCTATA+AGG | 0.358776 | 1.4:-69563145 | MS.gene07271:CDS |
| GCATGACATCTCCTCTTTCC+AGG | 0.359580 | 1.4:+69562094 | None:intergenic |
| TCTGCATGTGTTGAAGGTTC+TGG | 0.362025 | 1.4:-69564748 | MS.gene07271:CDS |
| GATTCTGCATTTCCTGAGAT+AGG | 0.362624 | 1.4:+69564421 | None:intergenic |
| AAACCATGCTCTTCATCATT+TGG | 0.365260 | 1.4:+69563413 | None:intergenic |
| CAGCTGTTCCTGAGGAGGAT+TGG | 0.366522 | 1.4:-69561996 | MS.gene07271:CDS |
| ATTTCCTGGTCTTGATAATT+GGG | 0.369870 | 1.4:-69564209 | MS.gene07271:CDS |
| TCAAAGAATGAAGGATTTCC+TGG | 0.374952 | 1.4:-69564223 | MS.gene07271:CDS |
| TCTTGGAGCCTCCATATTGA+TGG | 0.375100 | 1.4:+69562872 | None:intergenic |
| TGCAAAGAAGTTAGCGAAAT+CGG | 0.379072 | 1.4:-69563600 | MS.gene07271:CDS |
| TTGCGCGGTGACTTTCTAAT+CGG | 0.381507 | 1.4:+69564160 | None:intergenic |
| GTTGAGTACGTCTGCAAGCC+GGG | 0.382287 | 1.4:-69562279 | MS.gene07271:CDS |
| GATTTGGTTGTCTGCTTGAC+TGG | 0.387720 | 1.4:-69567420 | MS.gene07271:CDS |
| CTAATATGCTACAAATTTGA+AGG | 0.393357 | 1.4:-69567205 | MS.gene07271:intron |
| TGTTAATTTCTTCAGCTTGA+AGG | 0.393387 | 1.4:-69566159 | MS.gene07271:intron |
| TCAGCTGCCAAGAGAACTTT+GGG | 0.395676 | 1.4:-69564133 | MS.gene07271:CDS |
| TGAGGACGGTGCCATCAATA+TGG | 0.395743 | 1.4:-69562883 | MS.gene07271:CDS |
| AATTCCGGGTGCAAAAGATT+TGG | 0.397200 | 1.4:-69567436 | MS.gene07271:CDS |
| AAATCGACACGTGGCATGCC+AGG | 0.401546 | 1.4:+69562357 | None:intergenic |
| CTGATTTGTGGTGATGAAAC+TGG | 0.403537 | 1.4:-69561596 | MS.gene07271:CDS |
| AATCGGAAAAGGCAGTATTT+GGG | 0.404273 | 1.4:-69563583 | MS.gene07271:CDS |
| ATCACCGTTGGCTGGAAGAT+TGG | 0.404581 | 1.4:-69566304 | MS.gene07271:intron |
| TTCTCATCAGTGGTCATATC+AGG | 0.407810 | 1.4:-69563177 | MS.gene07271:CDS |
| ATGGTCCTAACATATGATTC+AGG | 0.408980 | 1.4:+69565090 | None:intergenic |
| ATTTCTTCAGCTTGAAGGAT+TGG | 0.411153 | 1.4:-69566154 | MS.gene07271:intron |
| ATTTGCAAAACTTCAAAGTA+AGG | 0.412007 | 1.4:-69562202 | MS.gene07271:CDS |
| CTTAAGCGTGCGGTCAAAGC+TGG | 0.412816 | 1.4:-69562456 | MS.gene07271:CDS |
| TTTCTTCAGCTTGAAGGATT+GGG | 0.414914 | 1.4:-69566153 | MS.gene07271:intron |
| GAGATTTACCATTTGTTAGT+GGG | 0.417900 | 1.4:+69564590 | None:intergenic |
| CTTGTGGACCAGTAGAATTT+GGG | 0.418686 | 1.4:+69565265 | None:intergenic |
| GGGATGCGCATTGTTGTATA+TGG | 0.419008 | 1.4:-69562804 | MS.gene07271:CDS |
| TGAGATTTACCATTTGTTAG+TGG | 0.420188 | 1.4:+69564589 | None:intergenic |
| CTTTATTGTGGCCAATCTCT+TGG | 0.421271 | 1.4:+69566334 | None:intergenic |
| AGCAAAGAAATAGCAACTTC+TGG | 0.422190 | 1.4:-69564013 | MS.gene07271:CDS |
| TCTACTTCACATTTGTCATC+TGG | 0.424117 | 1.4:+69563863 | None:intergenic |
| CACTCTTCGCGAGTCTTTCG+CGG | 0.424802 | 1.4:-69570260 | MS.gene07271:CDS |
| TGATGAAGAGCATGGTTTAG+AGG | 0.426899 | 1.4:-69563408 | MS.gene07271:CDS |
| TTCTGGTGTGAAGGAGCGTC+AGG | 0.432151 | 1.4:-69563996 | MS.gene07271:CDS |
| AACAATGCGCATCCCATAGA+AGG | 0.432187 | 1.4:+69562812 | None:intergenic |
| TGGCATGCCAGGGCTAACTA+TGG | 0.435261 | 1.4:+69562368 | None:intergenic |
| AATGGCTCCGGATGAAGAAT+TGG | 0.436222 | 1.4:-69563834 | MS.gene07271:CDS |
| ACTGGAAATACAAATTCATC+TGG | 0.437293 | 1.4:-69565012 | MS.gene07271:CDS |
| TTCAAGCTTGTTAATGGCGG+TGG | 0.438677 | 1.4:-69570088 | MS.gene07271:CDS |
| GTCAGCTGCCAAGAGAACTT+TGG | 0.438819 | 1.4:-69564134 | MS.gene07271:CDS |
| TAGAAAACTGTGCACCCATC+AGG | 0.438898 | 1.4:+69567257 | None:intergenic |
| TTTCTTTCTGTTGACAAAGA+TGG | 0.441291 | 1.4:-69564517 | MS.gene07271:CDS |
| TCAGACAATGGTTGGCCTGA+TGG | 0.442786 | 1.4:-69567272 | MS.gene07271:intron |
| CCCTTCCACCAAGGTAGAAA+AGG | 0.443209 | 1.4:-69564788 | MS.gene07271:CDS |
| TTAGAGGAGAGAAGAAATGT+TGG | 0.444297 | 1.4:-69563392 | MS.gene07271:CDS |
| CCATCAATTTGTTAACATCA+AGG | 0.444383 | 1.4:+69563960 | None:intergenic |
| TGCCACTCATAAGGCTCCTC+TGG | 0.446343 | 1.4:+69562921 | None:intergenic |
| CAGCTATTCCCGAGGAGGAT+TGG | 0.447918 | 1.4:-69561520 | MS.gene07271:CDS |
| TTCCCTCTTCAGACAATGGT+TGG | 0.449094 | 1.4:-69567280 | MS.gene07271:intron |
| ATCAGCATTCTTAGACTCAA+TGG | 0.450480 | 1.4:+69565071 | None:intergenic |
| ACTGTACGGCACTCATGCTT+TGG | 0.452603 | 1.4:-69562235 | MS.gene07271:CDS |
| ATACTCGCTTTCTTGATACT+GGG | 0.453049 | 1.4:-69562401 | MS.gene07271:CDS |
| GACAAATGTGAAGTAGAGTT+GGG | 0.453783 | 1.4:-69563857 | MS.gene07271:CDS |
| GCATGCCACGTGTCGATTTG+TGG | 0.463323 | 1.4:-69562353 | MS.gene07271:CDS |
| TCTTCTGCATACTCACTAAC+CGG | 0.463631 | 1.4:+69563929 | None:intergenic |
| CGCTAACTTCTTTGCAGCTG+TGG | 0.465086 | 1.4:+69563607 | None:intergenic |
| TCGGTGCTACGAGTTATTCT+AGG | 0.468156 | 1.4:-69564885 | MS.gene07271:CDS |
| GCATTTGATAAGCCTATCTC+AGG | 0.468199 | 1.4:-69564433 | MS.gene07271:CDS |
| CAGACAATGGTTGGCCTGAT+GGG | 0.468396 | 1.4:-69567271 | MS.gene07271:CDS |
| ATCAAATCTGCATGTGTTGA+AGG | 0.468581 | 1.4:-69564754 | MS.gene07271:CDS |
| AAATAACTTAAATACTTGCT+TGG | 0.468731 | 1.4:-69564491 | MS.gene07271:CDS |
| ATTCCAAGCTGGGGCAATCA+GGG | 0.469861 | 1.4:-69565386 | MS.gene07271:CDS |
| AGCTACCTGAATCATATGTT+AGG | 0.473090 | 1.4:-69565095 | MS.gene07271:CDS |
| TTTCCAGGGATCCACATACT+TGG | 0.474283 | 1.4:+69562109 | None:intergenic |
| TTGTGTTGTAGCGGACTACT+TGG | 0.476668 | 1.4:-69562301 | MS.gene07271:CDS |
| CGATCATCTCACAGAAAACA+AGG | 0.477136 | 1.4:-69570213 | MS.gene07271:intron |
| CCATAATGTCATCCCGATCC+TGG | 0.479361 | 1.4:+69567388 | None:intergenic |
| CATGCCACGTGTCGATTTGT+GGG | 0.481317 | 1.4:-69562352 | MS.gene07271:CDS |
| CTGCATGTGTTGAAGGTTCT+GGG | 0.482604 | 1.4:-69564747 | MS.gene07271:CDS |
| TGACAAATGTGAAGTAGAGT+TGG | 0.483171 | 1.4:-69563858 | MS.gene07271:CDS |
| TAATGGCGGTGGAGTTGATG+TGG | 0.483230 | 1.4:-69570077 | MS.gene07271:CDS |
| GGGCAAAACCAATCCTCCTC+GGG | 0.485220 | 1.4:+69561512 | None:intergenic |
| TTGATCTACTACCCTGGAAA+AGG | 0.486609 | 1.4:+69564843 | None:intergenic |
| GATGTTGGTGACTGCATTGT+GGG | 0.486729 | 1.4:-69569880 | MS.gene07271:CDS |
| TTCCAGTAATGTCAATATCC+TGG | 0.487192 | 1.4:+69565028 | None:intergenic |
| TGGCTATCTACGCCAGGATC+GGG | 0.488311 | 1.4:-69567400 | MS.gene07271:CDS |
| GAACCTGACAGCTTACCTTC+AGG | 0.489934 | 1.4:+69565148 | None:intergenic |
| GGGACAGACTCACTTTGCCT+TGG | 0.490392 | 1.4:+69561891 | None:intergenic |
| ACTAGAAATGATGGCGGAAG+AGG | 0.490800 | 1.4:-69565436 | MS.gene07271:CDS |
| GCTAGTTAATCACCGTTGGC+TGG | 0.491106 | 1.4:-69566312 | MS.gene07271:CDS |
| AGATGAACCAATTCTTCATC+CGG | 0.491955 | 1.4:+69563827 | None:intergenic |
| TTTCCCGCGATCCACATACT+TGG | 0.492525 | 1.4:+69561633 | None:intergenic |
| GTACGAGCTTGCCAAGAGAT+TGG | 0.493559 | 1.4:-69566345 | MS.gene07271:CDS |
| TCTAGAACCCAAAGTTCTCT+TGG | 0.493789 | 1.4:+69564126 | None:intergenic |
| TAGAGGAGAGAAGAAATGTT+GGG | 0.493960 | 1.4:-69563391 | MS.gene07271:CDS |
| GGTTGAGTACGTCTGCAAGC+CGG | 0.501698 | 1.4:-69562280 | MS.gene07271:CDS |
| CAACCTGAAGGTAAGCTGTC+AGG | 0.502452 | 1.4:-69565151 | MS.gene07271:CDS |
| GAAGGTTCTGGGAAAGAAAC+TGG | 0.504396 | 1.4:-69564736 | MS.gene07271:CDS |
| CAAACCGAGGTAGCATCAAC+AGG | 0.505536 | 1.4:+69569831 | None:intergenic |
| TGGCCATCGCCTACAGAGAA+AGG | 0.506713 | 1.4:-69563246 | MS.gene07271:CDS |
| GCACTCATGCTTTGGCAGAT+AGG | 0.508633 | 1.4:-69562227 | MS.gene07271:CDS |
| AATGGCGGTGGAGTTGATGT+GGG | 0.509620 | 1.4:-69570076 | MS.gene07271:CDS |
| GAGGCGGAAGATTCCAAGCT+GGG | 0.509729 | 1.4:-69565396 | MS.gene07271:CDS |
| ATTTACCAAACATCAAAGTA+AGG | 0.510575 | 1.4:-69561717 | MS.gene07271:CDS |
| TTTCTTAAGTGCTAGTAGCC+AGG | 0.510576 | 1.4:-69562958 | MS.gene07271:CDS |
| GGGCAAAACCAATCCTCCTC+AGG | 0.510620 | 1.4:+69561988 | None:intergenic |
| AAAACTACTCAGCGAGATGA+AGG | 0.510675 | 1.4:-69564055 | MS.gene07271:CDS |
| GATGAGCCACCTGTGTCTGA+TGG | 0.510751 | 1.4:-69564541 | MS.gene07271:CDS |
| CAGGTCATGGTGCCTTCTAT+GGG | 0.513908 | 1.4:-69562824 | MS.gene07271:CDS |
| TGATCAAGCGTTTGAAAGGA+AGG | 0.513959 | 1.4:-69563211 | MS.gene07271:CDS |
| GTTCTTATCACAGGACGATC+CGG | 0.515315 | 1.4:-69569931 | MS.gene07271:intron |
| ATAATTCATTGCCTCAAAAG+AGG | 0.515513 | 1.4:-69564690 | MS.gene07271:CDS |
| GGAGATTTGATGCTGATTTG+TGG | 0.515683 | 1.4:-69561608 | MS.gene07271:CDS |
| GAAGTTAGCGAAATCGGAAA+AGG | 0.517733 | 1.4:-69563594 | MS.gene07271:CDS |
| TTAATCAAGTCTAAAGATAG+TGG | 0.518083 | 1.4:-69564346 | MS.gene07271:CDS |
| GGCAGAAGAGATTATTGAGG+AGG | 0.521403 | 1.4:-69562181 | MS.gene07271:CDS |
| AGGATTGCAGCAATCGATAT+GGG | 0.522430 | 1.4:+69561550 | None:intergenic |
| GGAAAGAATTCAAAGAATGA+AGG | 0.523294 | 1.4:-69564232 | MS.gene07271:CDS |
| TCAACTTCTACAAGTGCTGA+TGG | 0.523391 | 1.4:-69564931 | MS.gene07271:CDS |
| CTGGCTATCTACGCCAGGAT+CGG | 0.524351 | 1.4:-69567401 | MS.gene07271:CDS |
| TTTGGCACAGCTGTGACTCA+TGG | 0.524555 | 1.4:-69565327 | MS.gene07271:CDS |
| CGTGCGGTCAAAGCTGGAGA+TGG | 0.526398 | 1.4:-69562450 | MS.gene07271:CDS |
| ATCAAATGCCAATGTGTTGG+AGG | 0.526782 | 1.4:+69564447 | None:intergenic |
| GTGATTAACTAGCTTTATTG+TGG | 0.528417 | 1.4:+69566322 | None:intergenic |
| GGCTAAGGATTCTGAGGATG+AGG | 0.530245 | 1.4:-69565415 | MS.gene07271:CDS |
| AATGAGACAAGAGATGAGGC+AGG | 0.530559 | 1.4:-69563548 | MS.gene07271:CDS |
| AATGATGGCGGAAGAGGCTA+AGG | 0.533098 | 1.4:-69565430 | MS.gene07271:CDS |
| AAAGCCATCCCAAATTCTAC+TGG | 0.533698 | 1.4:-69565273 | MS.gene07271:CDS |
| AGATTTACCATTTGTTAGTG+GGG | 0.534103 | 1.4:+69564591 | None:intergenic |
| CGTACAGTACGTGTCTCTCA+AGG | 0.534451 | 1.4:+69562250 | None:intergenic |
| GTATGTGGATCGCGGGAAAG+AGG | 0.535687 | 1.4:-69561629 | MS.gene07271:CDS |
| AAAGACTCTTCGATTACTAC+AGG | 0.536651 | 1.4:-69565219 | MS.gene07271:CDS |
| GTAGAATGCAAAGAAACGTC+AGG | 0.538051 | 1.4:+69565126 | None:intergenic |
| CTTGACTGGCTATCTACGCC+AGG | 0.538587 | 1.4:-69567406 | MS.gene07271:CDS |
| GGTTCCATTGGCTGTGGAGT+GGG | 0.539135 | 1.4:-69561575 | MS.gene07271:CDS |
| TCCGCTGGCAGCTGTTCCTG+AGG | 0.539542 | 1.4:-69562004 | MS.gene07271:CDS |
| GGTGATGAAACTGGTTCCAT+TGG | 0.539624 | 1.4:-69561587 | MS.gene07271:CDS |
| TGGGCAAAACCAATCCTCCT+CGG | 0.539703 | 1.4:+69561511 | None:intergenic |
| GAAGTTAGAAGCAGTTGACC+AGG | 0.539770 | 1.4:-69563774 | MS.gene07271:CDS |
| CAGGTGATCAAGCGTTTGAA+AGG | 0.545429 | 1.4:-69563215 | MS.gene07271:CDS |
| AGTAGAGTTGGGAATGGCTC+CGG | 0.546272 | 1.4:-69563846 | MS.gene07271:CDS |
| TTAAGTGCTAGTAGCCAGGC+AGG | 0.547195 | 1.4:-69562954 | MS.gene07271:CDS |
| TTCACATTGATCTACTACCC+TGG | 0.547336 | 1.4:+69564837 | None:intergenic |
| GAAAGTGGTTCCGTTGGCTG+TGG | 0.549655 | 1.4:-69562057 | MS.gene07271:CDS |
| TGTGTTGCTGCGAGAGAGGA+TGG | 0.552898 | 1.4:-69569906 | MS.gene07271:CDS |
| TTGCCAAGTATGTGGATCCC+TGG | 0.553257 | 1.4:-69562112 | MS.gene07271:CDS |
| TAAGGCAGAAGAGATTATTG+AGG | 0.553441 | 1.4:-69562184 | MS.gene07271:CDS |
| ACAGAGAAAGGAGTTTCAGC+AGG | 0.553933 | 1.4:-69563234 | MS.gene07271:CDS |
| CTTATCAAATGCCAATGTGT+TGG | 0.554180 | 1.4:+69564444 | None:intergenic |
| GTTGATGTGGGGAAGAACAC+TGG | 0.554212 | 1.4:-69570064 | MS.gene07271:CDS |
| TTGGGTTCTAGACCCAGAAA+AGG | 0.554581 | 1.4:-69564115 | MS.gene07271:CDS |
| GGAGATGTCATGCTGATTTG+TGG | 0.554732 | 1.4:-69562084 | MS.gene07271:CDS |
| CGACTGCTGCGATCCTCCGC+TGG | 0.556838 | 1.4:-69562019 | MS.gene07271:CDS |
| ATGTCAATATCCTGGAAGCC+AGG | 0.557352 | 1.4:+69565036 | None:intergenic |
| TAAGCCGTTGGTCGCAAACA+AGG | 0.557591 | 1.4:-69567236 | MS.gene07271:CDS |
| GTAACCTTGTTTGCGACCAA+CGG | 0.557635 | 1.4:+69567232 | None:intergenic |
| GACTATGCCATAGTTAGCCC+TGG | 0.558059 | 1.4:-69562375 | MS.gene07271:CDS |
| AAATTGTCATCACGCAGATT+AGG | 0.560313 | 1.4:+69564982 | None:intergenic |
| GGTACCCACTCCACAGCCAA+TGG | 0.560665 | 1.4:+69561571 | None:intergenic |
| CATGACATCTCCTCTTTCCA+GGG | 0.561920 | 1.4:+69562095 | None:intergenic |
| TTGCCAAGTATGTGGATCGC+GGG | 0.563072 | 1.4:-69561636 | MS.gene07271:CDS |
| TCCTCAGGAACAGCTGCCAG+CGG | 0.564075 | 1.4:+69562003 | None:intergenic |
| GCTGAAACTCCTTTCTCTGT+AGG | 0.564808 | 1.4:+69563237 | None:intergenic |
| AGAAACTGGTGTTATTCAAG+AGG | 0.565350 | 1.4:-69564722 | MS.gene07271:CDS |
| AGACCGCTTAAAGATTTGGA+TGG | 0.566779 | 1.4:-69567459 | MS.gene07271:CDS |
| GGTTCCGTTGGCTGTGGAGT+GGG | 0.567218 | 1.4:-69562051 | MS.gene07271:CDS |
| CGAGGAGGTCATTCCTCATG+AGG | 0.570925 | 1.4:-69561681 | MS.gene07271:CDS |
| TCAGGAACAGCTGCCAGCGG+AGG | 0.571501 | 1.4:+69562006 | None:intergenic |
| TGAGGCGGAAGATTCCAAGC+TGG | 0.572135 | 1.4:-69565397 | MS.gene07271:CDS |
| GGGTGCATTGTCTTCATCCA+AGG | 0.576286 | 1.4:-69561908 | MS.gene07271:CDS |
| ACTATAAGCAAAAGTCCCCT+AGG | 0.576594 | 1.4:-69565357 | MS.gene07271:CDS |
| AATAGCAACTTCTGGTGTGA+AGG | 0.576721 | 1.4:-69564005 | MS.gene07271:CDS |
| CATCTTGAGTGTTCATCACC+AGG | 0.578167 | 1.4:-69564253 | MS.gene07271:CDS |
| GCCACTTAAAATAAAACATG+TGG | 0.579555 | 1.4:+69563265 | None:intergenic |
| CTGAAGACAAATATAACAAG+AGG | 0.579867 | 1.4:-69566121 | MS.gene07271:intron |
| CTGATTTGTGGTGATGAAAG+TGG | 0.580324 | 1.4:-69562072 | MS.gene07271:CDS |
| GTATGTGGATCCCTGGAAAG+AGG | 0.581862 | 1.4:-69562105 | MS.gene07271:CDS |
| TTCTAACTTCTCTTCTGCCG+AGG | 0.582112 | 1.4:+69563787 | None:intergenic |
| TAAAGCTAGTTAATCACCGT+TGG | 0.584487 | 1.4:-69566316 | MS.gene07271:CDS |
| GAAACTGGTTCCATTGGCTG+TGG | 0.584504 | 1.4:-69561581 | MS.gene07271:CDS |
| CTTGAGAGACACGTACTGTA+CGG | 0.584559 | 1.4:-69562249 | MS.gene07271:CDS |
| CGGCTGTTGAAAGAAAGAAC+AGG | 0.584901 | 1.4:-69562843 | MS.gene07271:CDS |
| AGTGAGTATGCAGAAGATGC+TGG | 0.586456 | 1.4:-69563923 | MS.gene07271:CDS |
| AATGCCTGTTGATGCTACCT+CGG | 0.587295 | 1.4:-69569835 | MS.gene07271:intron |
| TTACCTTCCTGATGCAGTAG+CGG | 0.587895 | 1.4:+69563103 | None:intergenic |
| CTAACAAATGGTAAATCTCA+AGG | 0.589314 | 1.4:-69564586 | MS.gene07271:CDS |
| GATATAGCTTGCCAAGTATG+TGG | 0.590227 | 1.4:-69562120 | MS.gene07271:CDS |
| TCCAAGCTGGGGCAATCAGG+GGG | 0.590436 | 1.4:-69565384 | MS.gene07271:CDS |
| GAGTTCTCACTTATATCAGA+AGG | 0.591107 | 1.4:+69563491 | None:intergenic |
| CGATTCAAGCTTGTTAATGG+CGG | 0.592256 | 1.4:-69570091 | MS.gene07271:CDS |
| ACTCCTTTCTCTGTAGGCGA+TGG | 0.592552 | 1.4:+69563243 | None:intergenic |
| GCTCAAGGGACAGACTCACT+TGG | 0.593309 | 1.4:+69561415 | None:intergenic |
| GGGTGCATTATCTTCATCAA+AGG | 0.593538 | 1.4:-69561438 | MS.gene07271:CDS |
| TTGAAAGAAAGAACAGGTCA+TGG | 0.594190 | 1.4:-69562837 | MS.gene07271:CDS |
| TACTCGCTTTCTTGATACTG+GGG | 0.595273 | 1.4:-69562400 | MS.gene07271:CDS |
| ATATGGAGGCTCCAAGAAAG+TGG | 0.595831 | 1.4:-69562866 | MS.gene07271:CDS |
| AGCTTCTGCCTCCAACACAT+TGG | 0.597432 | 1.4:-69564455 | MS.gene07271:CDS |
| GATGTAGCTTGCCAAGTATG+TGG | 0.597538 | 1.4:-69561644 | MS.gene07271:CDS |
| GAGGTTGAGTCTAACTCTGA+AGG | 0.599018 | 1.4:+69563686 | None:intergenic |
| TTCCAAGCTGGGGCAATCAG+GGG | 0.599814 | 1.4:-69565385 | MS.gene07271:CDS |
| AGATGTTGGTGACTGCATTG+TGG | 0.600644 | 1.4:-69569881 | MS.gene07271:CDS |
| GCTGGCAGCTGTTCCTGAGG+AGG | 0.600700 | 1.4:-69562001 | MS.gene07271:CDS |
| GTCAAAATCTCTAAGAGAGG+AGG | 0.602945 | 1.4:-69565301 | MS.gene07271:CDS |
| TGGACCAGTAGAATTTGGGA+TGG | 0.603738 | 1.4:+69565269 | None:intergenic |
| TAGTGGTGAGCCTGATGCAA+CGG | 0.604279 | 1.4:-69564329 | MS.gene07271:CDS |
| TGGTTCCGTTGGCTGTGGAG+TGG | 0.604568 | 1.4:-69562052 | MS.gene07271:CDS |
| CAAGGTAGAAAAGGCAAGTG+AGG | 0.606134 | 1.4:-69564779 | MS.gene07271:CDS |
| GATCAAGCGTTTGAAAGGAA+GGG | 0.612539 | 1.4:-69563210 | MS.gene07271:CDS |
| GTTGAACATAGTGCTGATGT+TGG | 0.612914 | 1.4:-69569858 | MS.gene07271:CDS |
| AAATCCCAATTATCAAGACC+AGG | 0.613763 | 1.4:+69564205 | None:intergenic |
| TTCAAATAAAGTAGACACCT+CGG | 0.614340 | 1.4:-69563804 | MS.gene07271:CDS |
| TTGGCAGCTGACTTCTTGCG+CGG | 0.614710 | 1.4:+69564145 | None:intergenic |
| AGTACCCACTCCACAGCCAA+CGG | 0.616922 | 1.4:+69562047 | None:intergenic |
| TCATCGTCGCGATCCTCATG+AGG | 0.617058 | 1.4:+69562150 | None:intergenic |
| ATGTGAAGTAGAGTTGGGAA+TGG | 0.617643 | 1.4:-69563852 | MS.gene07271:CDS |
| AATCGACACGTGGCATGCCA+GGG | 0.619056 | 1.4:+69562358 | None:intergenic |
| TGTGTTGGAGGCAGAAGCTG+CGG | 0.620092 | 1.4:+69564459 | None:intergenic |
| TTGAGTACGTCTGCAAGCCG+GGG | 0.621599 | 1.4:-69562278 | MS.gene07271:CDS |
| GTACAGTACGTGTCTCTCAA+GGG | 0.622673 | 1.4:+69562251 | None:intergenic |
| GGTGGCTCATCAACCACAGA+TGG | 0.622968 | 1.4:+69564553 | None:intergenic |
| GGTGATGAAAGTGGTTCCGT+TGG | 0.623291 | 1.4:-69562063 | MS.gene07271:CDS |
| GGTGTGTGTTGCTGCGAGAG+AGG | 0.626024 | 1.4:-69569910 | MS.gene07271:CDS |
| TCATCATTGCAACCCTCATG+AGG | 0.626300 | 1.4:+69561668 | None:intergenic |
| AGACCAAATGATGAAGAGCA+TGG | 0.627980 | 1.4:-69563416 | MS.gene07271:CDS |
| TGGTTCCATTGGCTGTGGAG+TGG | 0.628638 | 1.4:-69561576 | MS.gene07271:CDS |
| GCATAATATTAGCAACCTGA+AGG | 0.628689 | 1.4:-69565163 | MS.gene07271:CDS |
| AAGTAGTCCGCTACAACACA+AGG | 0.628899 | 1.4:+69562303 | None:intergenic |
| TCAAGAAAGCGAGTATAAGG+AGG | 0.629991 | 1.4:+69562408 | None:intergenic |
| TCCAAATGAGACAAGAGATG+AGG | 0.630653 | 1.4:-69563552 | MS.gene07271:CDS |
| TTGCCGCTACTGCATCAGGA+AGG | 0.631958 | 1.4:-69563106 | MS.gene07271:intron |
| ATCGGAAAAGGCAGTATTTG+GGG | 0.632673 | 1.4:-69563582 | MS.gene07271:CDS |
| TTGTTAACATCAAGGATTTG+GGG | 0.635516 | 1.4:+69563968 | None:intergenic |
| GCACATTCAGATGAAAAGCT+CGG | 0.636983 | 1.4:-69564904 | MS.gene07271:CDS |
| ATCGTCAAAATCTCTAAGAG+AGG | 0.640134 | 1.4:-69565304 | MS.gene07271:CDS |
| GGAAGAGGCTAAGGATTCTG+AGG | 0.640663 | 1.4:-69565421 | MS.gene07271:CDS |
| GGCAGAAGAGATTATCGAGG+AGG | 0.641305 | 1.4:-69561696 | MS.gene07271:CDS |
| TCCTCGGGAATAGCTGTCAG+CGG | 0.641403 | 1.4:+69561527 | None:intergenic |
| TTTGCCGTGATTCTCATCAG+TGG | 0.642588 | 1.4:-69563187 | MS.gene07271:CDS |
| CTTGCCAAGTATGTGGATCG+CGG | 0.642622 | 1.4:-69561637 | MS.gene07271:CDS |
| GGCAGGAAAATTATTGCCAG+AGG | 0.644253 | 1.4:-69562937 | MS.gene07271:CDS |
| ATGAGACAAGAGATGAGGCA+GGG | 0.645233 | 1.4:-69563547 | MS.gene07271:CDS |
| TCGTCGCGATCCTCATGAGG+AGG | 0.645323 | 1.4:+69562153 | None:intergenic |
| GAAAGAAAGCCATCAGACAC+AGG | 0.645551 | 1.4:+69564532 | None:intergenic |
| ATGGCGGTGGAGTTGATGTG+GGG | 0.646548 | 1.4:-69570075 | MS.gene07271:CDS |
| ACAGGATACTCTTAAGCGTG+CGG | 0.647401 | 1.4:-69562466 | MS.gene07271:intron |
| ATTCCATCCAAATCTTTAAG+CGG | 0.647811 | 1.4:+69567456 | None:intergenic |
| GAGAGAGGATGGAAAGATGT+TGG | 0.648494 | 1.4:-69569895 | MS.gene07271:CDS |
| GGAGGTTATTCCTCCTCATG+AGG | 0.650168 | 1.4:-69562163 | MS.gene07271:CDS |
| TGAGATACCTTGTGTTGTAG+CGG | 0.650742 | 1.4:-69562310 | MS.gene07271:CDS |
| ATACAAATTCATCTGGTCTG+CGG | 0.652892 | 1.4:-69565005 | MS.gene07271:CDS |
| TAAGGATTCTGAGGATGAGG+CGG | 0.653839 | 1.4:-69565412 | MS.gene07271:CDS |
| TCTCTCGCAGCAACACACAC+CGG | 0.655994 | 1.4:+69569912 | None:intergenic |
| TCCGCTGACAGCTATTCCCG+AGG | 0.660558 | 1.4:-69561528 | MS.gene07271:CDS |
| GATCAATGTGAAGACAGAGA+AGG | 0.661317 | 1.4:-69564826 | MS.gene07271:CDS |
| TCGGGAATAGCTGTCAGCGG+AGG | 0.663496 | 1.4:+69561530 | None:intergenic |
| ATGACCACTGATGAGAATCA+CGG | 0.663566 | 1.4:+69563183 | None:intergenic |
| GAGGAGGTCATTCCTCATGA+GGG | 0.666729 | 1.4:-69561680 | MS.gene07271:CDS |
| TAAGGCAGAAGAGATTATCG+AGG | 0.671246 | 1.4:-69561699 | MS.gene07271:CDS |
| AGGCGGAAGATTCCAAGCTG+GGG | 0.675147 | 1.4:-69565395 | MS.gene07271:CDS |
| AGGATCGAAAGTGCCATCTG+TGG | 0.676967 | 1.4:-69564566 | MS.gene07271:CDS |
| GGACGGTGCCATCAATATGG+AGG | 0.679086 | 1.4:-69562880 | MS.gene07271:CDS |
| TGGAGGCTCCAAGAAAGTGG+CGG | 0.683408 | 1.4:-69562863 | MS.gene07271:CDS |
| TGCCAGAGGAGCCTTATGAG+TGG | 0.683644 | 1.4:-69562923 | MS.gene07271:CDS |
| TAGCTCCAGATCCTATAAGG+AGG | 0.687705 | 1.4:-69563142 | MS.gene07271:CDS |
| CATACCAATCTTCCAGCCAA+CGG | 0.689524 | 1.4:+69566300 | None:intergenic |
| TCTTGAAACCCCTTCCACCA+AGG | 0.691814 | 1.4:-69564797 | MS.gene07271:CDS |
| AGAAAGCCATCAGACACAGG+TGG | 0.698252 | 1.4:+69564535 | None:intergenic |
| GCTGACAGCTATTCCCGAGG+AGG | 0.706393 | 1.4:-69561525 | MS.gene07271:CDS |
| TGGACCCACAAATCGACACG+TGG | 0.713655 | 1.4:+69562348 | None:intergenic |
| TGATGAACACTCAAGATGTG+TGG | 0.746588 | 1.4:+69564258 | None:intergenic |
| TGTCTCTCAAGGGAGAACCC+CGG | 0.757252 | 1.4:+69562261 | None:intergenic |
| GAGTATGCAGAAGATGCTGG+TGG | 0.768912 | 1.4:-69563920 | MS.gene07271:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 69561430 | 69570297 | 69561430 | ID=MS.gene07271 |
| chr1.4 | mRNA | 69561430 | 69570297 | 69561430 | ID=MS.gene07271.t1;Parent=MS.gene07271 |
| chr1.4 | exon | 69570214 | 69570297 | 69570214 | ID=MS.gene07271.t1.exon1;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69570214 | 69570297 | 69570214 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69570027 | 69570116 | 69570027 | ID=MS.gene07271.t1.exon2;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69570027 | 69570116 | 69570027 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69569836 | 69569940 | 69569836 | ID=MS.gene07271.t1.exon3;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69569836 | 69569940 | 69569836 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69567389 | 69567490 | 69567389 | ID=MS.gene07271.t1.exon4;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69567389 | 69567490 | 69567389 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69567206 | 69567290 | 69567206 | ID=MS.gene07271.t1.exon5;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69567206 | 69567290 | 69567206 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69566305 | 69566374 | 69566305 | ID=MS.gene07271.t1.exon6;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69566305 | 69566374 | 69566305 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69566122 | 69566166 | 69566122 | ID=MS.gene07271.t1.exon7;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69566122 | 69566166 | 69566122 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69563107 | 69565467 | 69563107 | ID=MS.gene07271.t1.exon8;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69563107 | 69565467 | 69563107 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69562782 | 69562998 | 69562782 | ID=MS.gene07271.t1.exon9;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69562782 | 69562998 | 69562782 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69561904 | 69562484 | 69561904 | ID=MS.gene07271.t1.exon10;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69561904 | 69562484 | 69561904 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| chr1.4 | exon | 69561430 | 69561742 | 69561430 | ID=MS.gene07271.t1.exon11;Parent=MS.gene07271.t1 |
| chr1.4 | CDS | 69561430 | 69561742 | 69561430 | ID=cds.MS.gene07271.t1;Parent=MS.gene07271.t1 |
| Gene Sequence |
| Protein sequence |
