Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072982.t1 | XP_024635056.1 | 97.1 | 276 | 8 | 0 | 1 | 276 | 1 | 276 | 1.20E-143 | 519.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072982.t1 | A0A396IYW3 | 97.1 | 276 | 8 | 0 | 1 | 276 | 1 | 276 | 8.3e-144 | 519.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060349 | MS.gene072982 | 0.80567 | 1.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072982.t1 | MTR_3g108360 | 90.602 | 266 | 10 | 1 | 11 | 276 | 1 | 251 | 4.21e-155 | 478 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072982.t1 | AT2G25730 | 43.860 | 285 | 134 | 6 | 11 | 276 | 1 | 278 | 1.09e-60 | 208 |
| MS.gene072982.t1 | AT2G25730 | 43.860 | 285 | 134 | 6 | 11 | 276 | 1 | 278 | 1.29e-60 | 207 |
| MS.gene072982.t1 | AT2G25730 | 43.860 | 285 | 134 | 6 | 11 | 276 | 1 | 278 | 1.29e-60 | 207 |
| MS.gene072982.t1 | AT2G25730 | 43.860 | 285 | 134 | 6 | 11 | 276 | 1 | 278 | 1.41e-60 | 207 |
Find 54 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTCATTTGAATGCTATT+AGG | 0.241804 | 3.3:+88346445 | MS.gene072982:CDS |
| GAAATTGTGTACAAGAGTTT+TGG | 0.285162 | 3.3:+88346128 | MS.gene072982:CDS |
| GTTGATGAGTTTGAGGAAAT+TGG | 0.291733 | 3.3:+88346251 | MS.gene072982:CDS |
| TGTTCATTTGAATGCTATTA+GGG | 0.308768 | 3.3:+88346446 | MS.gene072982:CDS |
| CACTCTCGAATTGCTTCAAT+TGG | 0.323680 | 3.3:+88345981 | MS.gene072982:CDS |
| GCTGAAGACCAAGCGATGTT+AGG | 0.331772 | 3.3:-88345925 | None:intergenic |
| GGTTTAGAGGTTAATGTTAA+GGG | 0.339442 | 3.3:+88346354 | MS.gene072982:CDS |
| GATCAAGTCCATAATCAAAA+TGG | 0.339646 | 3.3:-88346521 | None:intergenic |
| TGGTTTAGAGGTTAATGTTA+AGG | 0.350244 | 3.3:+88346353 | MS.gene072982:CDS |
| CAGTGTGATCTTGAGGATAA+TGG | 0.355872 | 3.3:+88346333 | MS.gene072982:CDS |
| CAACGATAGATTGAAGAATA+TGG | 0.360270 | 3.3:-88345885 | None:intergenic |
| ATTCTTCAATCTATCGTTGC+TGG | 0.361502 | 3.3:+88345889 | MS.gene072982:CDS |
| ATTGAAGAATATGGCGAGTT+AGG | 0.370380 | 3.3:-88345876 | None:intergenic |
| CATCACCGCGATGCGACAAT+GGG | 0.389270 | 3.3:+88345748 | MS.gene072982:CDS |
| TGGATGACGCTTCCTCCGTT+TGG | 0.389678 | 3.3:+88346001 | MS.gene072982:CDS |
| TCTTGAGGATAATGGTTTAG+AGG | 0.389844 | 3.3:+88346341 | MS.gene072982:CDS |
| CAAGAAACTCCGCACGTAAA+CGG | 0.400239 | 3.3:-88346044 | None:intergenic |
| AAATTGAGTTGATGAGTTTG+AGG | 0.414697 | 3.3:+88346244 | MS.gene072982:CDS |
| GATAGATAGGTGAGAAGAGC+GGG | 0.415218 | 3.3:-88345958 | None:intergenic |
| GAAGGAGGATGTTGATGTTA+AGG | 0.422080 | 3.3:+88346392 | MS.gene072982:CDS |
| TTAGGTCACGGTTTCGCGTT+CGG | 0.427044 | 3.3:-88345858 | None:intergenic |
| CAATTCGAGAGTGGATAGAT+AGG | 0.432777 | 3.3:-88345971 | None:intergenic |
| GCTATTAGGGAGAGTCTTGA+AGG | 0.433073 | 3.3:+88346459 | MS.gene072982:CDS |
| GCATCACCGCGATGCGACAA+TGG | 0.454654 | 3.3:+88345747 | MS.gene072982:CDS |
| CTCAATTCCCTAACATCGCT+TGG | 0.455664 | 3.3:+88345917 | MS.gene072982:CDS |
| CTGAAGACCAAGCGATGTTA+GGG | 0.458529 | 3.3:-88345924 | None:intergenic |
| GACGAGACGACAGAGGGTTT+CGG | 0.463794 | 3.3:-88345779 | None:intergenic |
| GGGTGGTGATGATGATTTGA+AGG | 0.481711 | 3.3:+88346374 | MS.gene072982:CDS |
| AGCGAGAAGAACGCCGCGTA+AGG | 0.487890 | 3.3:-88345833 | None:intergenic |
| CTTGAAGGTGGTGATGTTGA+AGG | 0.510651 | 3.3:+88346474 | MS.gene072982:CDS |
| CGCACGTAAACGGAGCGATT+CGG | 0.513464 | 3.3:-88346034 | None:intergenic |
| AGGTTGAGAGTTGATGAAAA+TGG | 0.518397 | 3.3:+88346177 | MS.gene072982:CDS |
| TGGGATACAGAAGATGGCTC+AGG | 0.523615 | 3.3:+88346422 | MS.gene072982:CDS |
| GACTTGATCAATCTGAATAC+CGG | 0.529690 | 3.3:+88346535 | MS.gene072982:CDS |
| TTAGAGGTTAATGTTAAGGG+TGG | 0.548119 | 3.3:+88346357 | MS.gene072982:CDS |
| GGATAGATAGGTGAGAAGAG+CGG | 0.548120 | 3.3:-88345959 | None:intergenic |
| GATTAGACAGTGTGATCTTG+AGG | 0.561517 | 3.3:+88346326 | MS.gene072982:CDS |
| TTCGGAATCAAAATTCCAAA+CGG | 0.572661 | 3.3:-88346016 | None:intergenic |
| GCGAGAAGAACGCCGCGTAA+GGG | 0.583308 | 3.3:-88345832 | None:intergenic |
| TTGAAGGTGGTGATGTTGAA+GGG | 0.595760 | 3.3:+88346475 | MS.gene072982:CDS |
| ATTCGCGACGAGACGACAGA+GGG | 0.610207 | 3.3:-88345785 | None:intergenic |
| GAATATGGCGAGTTAGGTCA+CGG | 0.617138 | 3.3:-88345870 | None:intergenic |
| CAATTGAAGCAATTCGAGAG+TGG | 0.626205 | 3.3:-88345980 | None:intergenic |
| GGAGGAAAATGATGATGATG+AGG | 0.633633 | 3.3:+88346104 | MS.gene072982:CDS |
| CGAATCGCTCCGTTTACGTG+CGG | 0.643134 | 3.3:+88346035 | MS.gene072982:CDS |
| CTTTCCCCATTGTCGCATCG+CGG | 0.645259 | 3.3:-88345753 | None:intergenic |
| TGAAGGTGGTGATGTTGAAG+GGG | 0.657124 | 3.3:+88346476 | MS.gene072982:CDS |
| GGAATCAAAATTCCAAACGG+AGG | 0.660291 | 3.3:-88346013 | None:intergenic |
| GATTCGCGACGAGACGACAG+AGG | 0.687705 | 3.3:-88345786 | None:intergenic |
| TGGTGATGATGATTTGAAGG+AGG | 0.687782 | 3.3:+88346377 | MS.gene072982:CDS |
| ATCACCGCGATGCGACAATG+GGG | 0.697114 | 3.3:+88345749 | MS.gene072982:CDS |
| AGCGATGTTAGGGAATTGAG+CGG | 0.711377 | 3.3:-88345914 | None:intergenic |
| GCTCAATTCGAACCCTTACG+CGG | 0.712099 | 3.3:+88345820 | MS.gene072982:CDS |
| ATTAGGGAGAGTCTTGAAGG+TGG | 0.713638 | 3.3:+88346462 | MS.gene072982:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTTCATTTGAATGCTATTA+GGG | + | chr3.3:88346446-88346465 | MS.gene072982:CDS | 25.0% |
| ! | TTGTTCATTTGAATGCTATT+AGG | + | chr3.3:88346445-88346464 | MS.gene072982:CDS | 25.0% |
| CAACGATAGATTGAAGAATA+TGG | - | chr3.3:88345888-88345907 | None:intergenic | 30.0% | |
| GATCAAGTCCATAATCAAAA+TGG | - | chr3.3:88346524-88346543 | None:intergenic | 30.0% | |
| GGTTTAGAGGTTAATGTTAA+GGG | + | chr3.3:88346354-88346373 | MS.gene072982:CDS | 30.0% | |
| TGGTTTAGAGGTTAATGTTA+AGG | + | chr3.3:88346353-88346372 | MS.gene072982:CDS | 30.0% | |
| TTCGGAATCAAAATTCCAAA+CGG | - | chr3.3:88346019-88346038 | None:intergenic | 30.0% | |
| ! | GAAATTGTGTACAAGAGTTT+TGG | + | chr3.3:88346128-88346147 | MS.gene072982:CDS | 30.0% |
| !! | AAATTGAGTTGATGAGTTTG+AGG | + | chr3.3:88346244-88346263 | MS.gene072982:CDS | 30.0% |
| !! | GCATTTGATTGATTTTGTGT+CGG | + | chr3.3:88346077-88346096 | MS.gene072982:CDS | 30.0% |
| !! | TTGATGTTAAGGTGCTTTTT+GGG | + | chr3.3:88346403-88346422 | MS.gene072982:CDS | 30.0% |
| !!! | CGTTTTCTCCATTTTGATTA+TGG | + | chr3.3:88346513-88346532 | MS.gene072982:CDS | 30.0% |
| !!! | TTTTGGAGTTAGGTTTTAAG+AGG | + | chr3.3:88346157-88346176 | MS.gene072982:CDS | 30.0% |
| ATTCTTCAATCTATCGTTGC+TGG | + | chr3.3:88345889-88345908 | MS.gene072982:CDS | 35.0% | |
| ATTGAAGAATATGGCGAGTT+AGG | - | chr3.3:88345879-88345898 | None:intergenic | 35.0% | |
| GACTTGATCAATCTGAATAC+CGG | + | chr3.3:88346535-88346554 | MS.gene072982:CDS | 35.0% | |
| TTAGAGGTTAATGTTAAGGG+TGG | + | chr3.3:88346357-88346376 | MS.gene072982:CDS | 35.0% | |
| ! | GTTGATGAGTTTGAGGAAAT+TGG | + | chr3.3:88346251-88346270 | MS.gene072982:CDS | 35.0% |
| ! | TCTTGAGGATAATGGTTTAG+AGG | + | chr3.3:88346341-88346360 | MS.gene072982:CDS | 35.0% |
| !! | AGGTTGAGAGTTGATGAAAA+TGG | + | chr3.3:88346177-88346196 | MS.gene072982:CDS | 35.0% |
| !! | GTTGATGTTAAGGTGCTTTT+TGG | + | chr3.3:88346402-88346421 | MS.gene072982:CDS | 35.0% |
| !!! | AAGAGTTTTGGATAGGGTTT+TGG | + | chr3.3:88346140-88346159 | MS.gene072982:CDS | 35.0% |
| !!! | GTGTACAAGAGTTTTGGATA+GGG | + | chr3.3:88346134-88346153 | MS.gene072982:CDS | 35.0% |
| !!! | TGTGTACAAGAGTTTTGGAT+AGG | + | chr3.3:88346133-88346152 | MS.gene072982:CDS | 35.0% |
| CAATTCGAGAGTGGATAGAT+AGG | - | chr3.3:88345974-88345993 | None:intergenic | 40.0% | |
| CAATTGAAGCAATTCGAGAG+TGG | - | chr3.3:88345983-88346002 | None:intergenic | 40.0% | |
| CACTCTCGAATTGCTTCAAT+TGG | + | chr3.3:88345981-88346000 | MS.gene072982:CDS | 40.0% | |
| CAGTGTGATCTTGAGGATAA+TGG | + | chr3.3:88346333-88346352 | MS.gene072982:CDS | 40.0% | |
| GAAGGAGGATGTTGATGTTA+AGG | + | chr3.3:88346392-88346411 | MS.gene072982:CDS | 40.0% | |
| GATTAGACAGTGTGATCTTG+AGG | + | chr3.3:88346326-88346345 | MS.gene072982:CDS | 40.0% | |
| GGAATCAAAATTCCAAACGG+AGG | - | chr3.3:88346016-88346035 | None:intergenic | 40.0% | |
| ! | GGAGGAAAATGATGATGATG+AGG | + | chr3.3:88346104-88346123 | MS.gene072982:CDS | 40.0% |
| ! | TGGTGATGATGATTTGAAGG+AGG | + | chr3.3:88346377-88346396 | MS.gene072982:CDS | 40.0% |
| ! | TTGAAGGTGGTGATGTTGAA+GGG | + | chr3.3:88346475-88346494 | MS.gene072982:CDS | 40.0% |
| !! | GATTGATTTTGTGTCGGAGA+AGG | + | chr3.3:88346083-88346102 | MS.gene072982:CDS | 40.0% |
| !!! | GCTTTTTGGGATACAGAAGA+TGG | + | chr3.3:88346416-88346435 | MS.gene072982:CDS | 40.0% |
| !!! | TTGGATAGGGTTTTGGAGTT+AGG | + | chr3.3:88346147-88346166 | MS.gene072982:CDS | 40.0% |
| AGCGATGTTAGGGAATTGAG+CGG | - | chr3.3:88345917-88345936 | None:intergenic | 45.0% | |
| ATTAGGGAGAGTCTTGAAGG+TGG | + | chr3.3:88346462-88346481 | MS.gene072982:CDS | 45.0% | |
| CAAGAAACTCCGCACGTAAA+CGG | - | chr3.3:88346047-88346066 | None:intergenic | 45.0% | |
| CTCAATTCCCTAACATCGCT+TGG | + | chr3.3:88345917-88345936 | MS.gene072982:CDS | 45.0% | |
| CTGAAGACCAAGCGATGTTA+GGG | - | chr3.3:88345927-88345946 | None:intergenic | 45.0% | |
| GAATATGGCGAGTTAGGTCA+CGG | - | chr3.3:88345873-88345892 | None:intergenic | 45.0% | |
| GATAGATAGGTGAGAAGAGC+GGG | - | chr3.3:88345961-88345980 | None:intergenic | 45.0% | |
| GCTATTAGGGAGAGTCTTGA+AGG | + | chr3.3:88346459-88346478 | MS.gene072982:CDS | 45.0% | |
| GGATAGATAGGTGAGAAGAG+CGG | - | chr3.3:88345962-88345981 | None:intergenic | 45.0% | |
| ! | CTTGAAGGTGGTGATGTTGA+AGG | + | chr3.3:88346474-88346493 | MS.gene072982:CDS | 45.0% |
| ! | GGGTGGTGATGATGATTTGA+AGG | + | chr3.3:88346374-88346393 | MS.gene072982:CDS | 45.0% |
| ! | TGAAGGTGGTGATGTTGAAG+GGG | + | chr3.3:88346476-88346495 | MS.gene072982:CDS | 45.0% |
| ! | TGATTTTGTGTCGGAGAAGG+AGG | + | chr3.3:88346086-88346105 | MS.gene072982:CDS | 45.0% |
| GCTCAATTCGAACCCTTACG+CGG | + | chr3.3:88345820-88345839 | MS.gene072982:CDS | 50.0% | |
| GCTGAAGACCAAGCGATGTT+AGG | - | chr3.3:88345928-88345947 | None:intergenic | 50.0% | |
| TGGGATACAGAAGATGGCTC+AGG | + | chr3.3:88346422-88346441 | MS.gene072982:CDS | 50.0% | |
| TTAGGTCACGGTTTCGCGTT+CGG | - | chr3.3:88345861-88345880 | None:intergenic | 50.0% | |
| ATCACCGCGATGCGACAATG+GGG | + | chr3.3:88345749-88345768 | MS.gene072982:CDS | 55.0% | |
| ATTCGCGACGAGACGACAGA+GGG | - | chr3.3:88345788-88345807 | None:intergenic | 55.0% | |
| CATCACCGCGATGCGACAAT+GGG | + | chr3.3:88345748-88345767 | MS.gene072982:CDS | 55.0% | |
| CGAATCGCTCCGTTTACGTG+CGG | + | chr3.3:88346035-88346054 | MS.gene072982:CDS | 55.0% | |
| CGCACGTAAACGGAGCGATT+CGG | - | chr3.3:88346037-88346056 | None:intergenic | 55.0% | |
| CTTTCCCCATTGTCGCATCG+CGG | - | chr3.3:88345756-88345775 | None:intergenic | 55.0% | |
| GACGAGACGACAGAGGGTTT+CGG | - | chr3.3:88345782-88345801 | None:intergenic | 55.0% | |
| TGGATGACGCTTCCTCCGTT+TGG | + | chr3.3:88346001-88346020 | MS.gene072982:CDS | 55.0% | |
| AGCGAGAAGAACGCCGCGTA+AGG | - | chr3.3:88345836-88345855 | None:intergenic | 60.0% | |
| GATTCGCGACGAGACGACAG+AGG | - | chr3.3:88345789-88345808 | None:intergenic | 60.0% | |
| GCATCACCGCGATGCGACAA+TGG | + | chr3.3:88345747-88345766 | MS.gene072982:CDS | 60.0% | |
| GCGAGAAGAACGCCGCGTAA+GGG | - | chr3.3:88345835-88345854 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 88345736 | 88346572 | 88345736 | ID=MS.gene072982 |
| chr3.3 | mRNA | 88345736 | 88346572 | 88345736 | ID=MS.gene072982.t1;Parent=MS.gene072982 |
| chr3.3 | exon | 88345736 | 88346572 | 88345736 | ID=MS.gene072982.t1.exon1;Parent=MS.gene072982.t1 |
| chr3.3 | CDS | 88345736 | 88346572 | 88345736 | ID=cds.MS.gene072982.t1;Parent=MS.gene072982.t1 |
| Gene Sequence |
| Protein sequence |
