Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073013.t1 | XP_003603581.1 | 99.3 | 140 | 1 | 0 | 1 | 140 | 40 | 179 | 1.60E-64 | 255.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073013.t1 | Q9SVG9 | 57.1 | 63 | 27 | 0 | 77 | 139 | 121 | 183 | 1.2e-12 | 74.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073013.t1 | G7J2W3 | 99.3 | 140 | 1 | 0 | 1 | 140 | 40 | 179 | 1.1e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052463 | MS.gene073013 | 0.800595 | 1.34E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073013.t1 | MTR_3g109320 | 99.286 | 140 | 1 | 0 | 1 | 140 | 40 | 179 | 7.56e-96 | 274 |
| MS.gene073013.t1 | MTR_3g109985 | 93.571 | 140 | 9 | 0 | 1 | 140 | 40 | 179 | 1.24e-76 | 225 |
| MS.gene073013.t1 | MTR_3g110008 | 93.571 | 140 | 9 | 0 | 1 | 140 | 40 | 179 | 1.56e-76 | 225 |
| MS.gene073013.t1 | MTR_7g074240 | 54.930 | 71 | 32 | 0 | 69 | 139 | 100 | 170 | 1.31e-16 | 72.4 |
| MS.gene073013.t1 | MTR_7g011010 | 37.931 | 116 | 63 | 2 | 21 | 135 | 86 | 193 | 4.73e-15 | 68.9 |
| MS.gene073013.t1 | MTR_5g025690 | 36.667 | 120 | 67 | 2 | 21 | 139 | 84 | 195 | 7.41e-14 | 65.9 |
| MS.gene073013.t1 | MTR_4g082050 | 39.316 | 117 | 61 | 2 | 23 | 139 | 32 | 138 | 1.51e-13 | 63.9 |
| MS.gene073013.t1 | MTR_4g127560 | 35.294 | 119 | 70 | 1 | 17 | 135 | 78 | 189 | 3.39e-12 | 61.2 |
| MS.gene073013.t1 | MTR_4g067270 | 32.394 | 142 | 89 | 3 | 4 | 139 | 1 | 141 | 8.24e-12 | 59.3 |
| MS.gene073013.t1 | MTR_7g089760 | 46.667 | 60 | 32 | 0 | 80 | 139 | 82 | 141 | 8.74e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073013.t1 | AT4G20780 | 57.143 | 63 | 27 | 0 | 77 | 139 | 121 | 183 | 2.46e-16 | 72.0 |
| MS.gene073013.t1 | AT5G44460 | 50.667 | 75 | 36 | 1 | 65 | 139 | 101 | 174 | 9.46e-16 | 70.5 |
| MS.gene073013.t1 | AT3G29000 | 38.182 | 110 | 59 | 1 | 26 | 135 | 85 | 185 | 9.83e-16 | 70.5 |
| MS.gene073013.t1 | AT2G43290 | 31.757 | 148 | 89 | 3 | 3 | 139 | 60 | 206 | 2.29e-13 | 64.7 |
| MS.gene073013.t1 | AT3G47480 | 41.732 | 127 | 60 | 6 | 20 | 135 | 51 | 174 | 3.84e-13 | 63.5 |
| MS.gene073013.t1 | AT1G05990 | 47.541 | 61 | 32 | 0 | 79 | 139 | 82 | 142 | 3.49e-12 | 60.5 |
| MS.gene073013.t1 | AT5G39670 | 34.545 | 110 | 65 | 2 | 26 | 135 | 93 | 195 | 7.45e-12 | 60.5 |
Find 33 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGCTCTTGTTACTGTCTT+TGG | 0.208211 | 3.4:-94271235 | MS.gene073013:CDS |
| TTTGATGGAAAAGTTGATTT+TGG | 0.233481 | 3.4:-94270887 | MS.gene073013:CDS |
| GAGAAGCTTGGTTTGATCTA+TGG | 0.288032 | 3.4:-94271163 | MS.gene073013:CDS |
| GATTGCTTGGGTTTGGATAA+AGG | 0.333333 | 3.4:-94270959 | MS.gene073013:CDS |
| GTTGTTGATTTGAACTTTGA+TGG | 0.338891 | 3.4:-94270902 | MS.gene073013:CDS |
| TGAAGAGGTGCTTGGTGAAT+TGG | 0.379686 | 3.4:-94271083 | MS.gene073013:CDS |
| TACAATTGAAAGGATGGTTA+AGG | 0.418076 | 3.4:-94270924 | MS.gene073013:CDS |
| AAGAGAGTGCTTGATTGCTT+GGG | 0.427610 | 3.4:-94270971 | MS.gene073013:CDS |
| CTTGGGTTTGGATAAAGGTT+GGG | 0.431362 | 3.4:-94270954 | MS.gene073013:CDS |
| AGTGCTTGATTGCTTGGGTT+TGG | 0.431577 | 3.4:-94270966 | MS.gene073013:CDS |
| GCTTGGGTTTGGATAAAGGT+TGG | 0.438195 | 3.4:-94270955 | MS.gene073013:CDS |
| GAAGAGAGTGCTTGATTGCT+TGG | 0.450516 | 3.4:-94270972 | MS.gene073013:CDS |
| TTCTTCATCATCATCTTGAA+AGG | 0.470906 | 3.4:+94271117 | None:intergenic |
| GTCTTTGGAATGGAAACAAA+TGG | 0.472415 | 3.4:-94271220 | MS.gene073013:CDS |
| GATCTATGGATCTGATCAAA+AGG | 0.484438 | 3.4:-94271149 | MS.gene073013:CDS |
| TATGAATACAATTGAAAGGA+TGG | 0.486100 | 3.4:-94270930 | MS.gene073013:CDS |
| TGGTGAGTTTGAATTGATGA+TGG | 0.492217 | 3.4:-94270867 | MS.gene073013:CDS |
| TTCAAAATATTTGATGAGGA+TGG | 0.511001 | 3.4:-94271022 | MS.gene073013:CDS |
| AGGCAAGTGGTGGAGAAGCT+TGG | 0.515598 | 3.4:-94271175 | MS.gene073013:CDS |
| TCTTGTTACTGTCTTTGGAA+TGG | 0.529567 | 3.4:-94271230 | MS.gene073013:CDS |
| GGGATATGAATACAATTGAA+AGG | 0.561378 | 3.4:-94270934 | MS.gene073013:CDS |
| GAATCAAGAAGGAAAATGCT+AGG | 0.572252 | 3.4:-94271195 | MS.gene073013:CDS |
| GTGGTTGTTGAAGAGGTGCT+TGG | 0.573531 | 3.4:-94271091 | MS.gene073013:CDS |
| AGAAGAAGTGGTTGTTGAAG+AGG | 0.574658 | 3.4:-94271098 | MS.gene073013:CDS |
| GGTGAGTTTGAATTGATGAT+GGG | 0.583460 | 3.4:-94270866 | MS.gene073013:CDS |
| AACAAATGGAAGAATCAAGA+AGG | 0.586852 | 3.4:-94271206 | MS.gene073013:CDS |
| AGCTTTCAAAATATTTGATG+AGG | 0.590504 | 3.4:-94271026 | MS.gene073013:CDS |
| ATATTTGATGAGGATGGTGA+TGG | 0.599032 | 3.4:-94271016 | MS.gene073013:CDS |
| AGATGATGATGAAGAAGAAG+TGG | 0.599126 | 3.4:-94271110 | MS.gene073013:CDS |
| ATATGCTCAAGCTCGACGAA+AGG | 0.626001 | 3.4:-94271264 | MS.gene073013:CDS |
| GGAAAATGCTAGGCAAGTGG+TGG | 0.637309 | 3.4:-94271185 | MS.gene073013:CDS |
| TGATGGATATATAGATGCAA+TGG | 0.640545 | 3.4:-94270999 | MS.gene073013:CDS |
| GAAGGAAAATGCTAGGCAAG+TGG | 0.650638 | 3.4:-94271188 | MS.gene073013:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGCTTTCAAAATATTTGATG+AGG | - | chr3.4:94271098-94271117 | MS.gene073013:CDS | 25.0% |
| ! | TATGAATACAATTGAAAGGA+TGG | - | chr3.4:94271194-94271213 | MS.gene073013:CDS | 25.0% |
| ! | TTCAAAATATTTGATGAGGA+TGG | - | chr3.4:94271102-94271121 | MS.gene073013:CDS | 25.0% |
| !!! | TTTGATGGAAAAGTTGATTT+TGG | - | chr3.4:94271237-94271256 | MS.gene073013:CDS | 25.0% |
| AACAAATGGAAGAATCAAGA+AGG | - | chr3.4:94270918-94270937 | MS.gene073013:CDS | 30.0% | |
| GGGATATGAATACAATTGAA+AGG | - | chr3.4:94271190-94271209 | MS.gene073013:CDS | 30.0% | |
| TACAATTGAAAGGATGGTTA+AGG | - | chr3.4:94271200-94271219 | MS.gene073013:CDS | 30.0% | |
| TTCTTCATCATCATCTTGAA+AGG | + | chr3.4:94271010-94271029 | None:intergenic | 30.0% | |
| ! | TGATGGATATATAGATGCAA+TGG | - | chr3.4:94271125-94271144 | MS.gene073013:CDS | 30.0% |
| !! | GTTGTTGATTTGAACTTTGA+TGG | - | chr3.4:94271222-94271241 | MS.gene073013:CDS | 30.0% |
| ATATTTGATGAGGATGGTGA+TGG | - | chr3.4:94271108-94271127 | MS.gene073013:CDS | 35.0% | |
| GAATCAAGAAGGAAAATGCT+AGG | - | chr3.4:94270929-94270948 | MS.gene073013:CDS | 35.0% | |
| GATCTATGGATCTGATCAAA+AGG | - | chr3.4:94270975-94270994 | MS.gene073013:CDS | 35.0% | |
| TGGTGAGTTTGAATTGATGA+TGG | - | chr3.4:94271257-94271276 | MS.gene073013:CDS | 35.0% | |
| ! | AGATGATGATGAAGAAGAAG+TGG | - | chr3.4:94271014-94271033 | MS.gene073013:CDS | 35.0% |
| ! | GGTGAGTTTGAATTGATGAT+GGG | - | chr3.4:94271258-94271277 | MS.gene073013:CDS | 35.0% |
| ! | TCTTGTTACTGTCTTTGGAA+TGG | - | chr3.4:94270894-94270913 | MS.gene073013:CDS | 35.0% |
| !! | GTCTTTGGAATGGAAACAAA+TGG | - | chr3.4:94270904-94270923 | MS.gene073013:CDS | 35.0% |
| AGAAGAAGTGGTTGTTGAAG+AGG | - | chr3.4:94271026-94271045 | MS.gene073013:CDS | 40.0% | |
| CTTGGGTTTGGATAAAGGTT+GGG | - | chr3.4:94271170-94271189 | MS.gene073013:CDS | 40.0% | |
| GATTGCTTGGGTTTGGATAA+AGG | - | chr3.4:94271165-94271184 | MS.gene073013:CDS | 40.0% | |
| ! | GAGAAGCTTGGTTTGATCTA+TGG | - | chr3.4:94270961-94270980 | MS.gene073013:CDS | 40.0% |
| !! | AAGAGAGTGCTTGATTGCTT+GGG | - | chr3.4:94271153-94271172 | MS.gene073013:CDS | 40.0% |
| AGTGCTTGATTGCTTGGGTT+TGG | - | chr3.4:94271158-94271177 | MS.gene073013:CDS | 45.0% | |
| CGAGCTCTTGTTACTGTCTT+TGG | - | chr3.4:94270889-94270908 | MS.gene073013:CDS | 45.0% | |
| GAAGGAAAATGCTAGGCAAG+TGG | - | chr3.4:94270936-94270955 | MS.gene073013:CDS | 45.0% | |
| GCTTGGGTTTGGATAAAGGT+TGG | - | chr3.4:94271169-94271188 | MS.gene073013:CDS | 45.0% | |
| !! | GAAGAGAGTGCTTGATTGCT+TGG | - | chr3.4:94271152-94271171 | MS.gene073013:CDS | 45.0% |
| !! | TGAAGAGGTGCTTGGTGAAT+TGG | - | chr3.4:94271041-94271060 | MS.gene073013:CDS | 45.0% |
| GGAAAATGCTAGGCAAGTGG+TGG | - | chr3.4:94270939-94270958 | MS.gene073013:CDS | 50.0% | |
| !! | GTGGTTGTTGAAGAGGTGCT+TGG | - | chr3.4:94271033-94271052 | MS.gene073013:CDS | 50.0% |
| ! | AGGCAAGTGGTGGAGAAGCT+TGG | - | chr3.4:94270949-94270968 | MS.gene073013:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 94270862 | 94271284 | 94270862 | ID=MS.gene073013 |
| chr3.4 | mRNA | 94270862 | 94271284 | 94270862 | ID=MS.gene073013.t1;Parent=MS.gene073013 |
| chr3.4 | exon | 94270862 | 94271284 | 94270862 | ID=MS.gene073013.t1.exon1;Parent=MS.gene073013.t1 |
| chr3.4 | CDS | 94270862 | 94271284 | 94270862 | ID=cds.MS.gene073013.t1;Parent=MS.gene073013.t1 |
| Gene Sequence |
| Protein sequence |
