Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073761.t1 | XP_013468213.1 | 91 | 467 | 35 | 1 | 1 | 467 | 17 | 476 | 2.60E-241 | 844.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073761.t1 | A0A072VVY5 | 91.0 | 467 | 35 | 1 | 1 | 467 | 17 | 476 | 1.9e-241 | 844.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059865 | MS.gene073761 | -0.801214 | 1.00E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073761.t1 | MTR_1g064690 | 92.094 | 468 | 30 | 1 | 1 | 468 | 17 | 477 | 0.0 | 854 |
| MS.gene073761.t1 | MTR_7g101130 | 50.522 | 479 | 184 | 18 | 3 | 457 | 17 | 466 | 8.39e-136 | 399 |
| MS.gene073761.t1 | MTR_7g070530 | 25.056 | 443 | 245 | 17 | 79 | 457 | 90 | 509 | 2.67e-27 | 114 |
| MS.gene073761.t1 | MTR_4g013290 | 23.788 | 433 | 259 | 14 | 79 | 457 | 94 | 509 | 3.42e-21 | 96.7 |
| MS.gene073761.t1 | MTR_3g031200 | 24.371 | 517 | 296 | 22 | 1 | 456 | 11 | 493 | 1.06e-20 | 95.1 |
| MS.gene073761.t1 | MTR_3g100550 | 23.939 | 330 | 176 | 14 | 145 | 457 | 147 | 418 | 2.03e-13 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073761.t1 | AT3G53400 | 41.401 | 471 | 237 | 13 | 3 | 457 | 17 | 464 | 4.79e-105 | 321 |
| MS.gene073761.t1 | AT5G03190 | 39.703 | 471 | 230 | 15 | 3 | 457 | 17 | 449 | 1.14e-99 | 306 |
| MS.gene073761.t1 | AT5G03190 | 39.619 | 472 | 231 | 15 | 2 | 457 | 40 | 473 | 1.56e-99 | 307 |
| MS.gene073761.t1 | AT1G24480 | 29.670 | 182 | 110 | 6 | 63 | 236 | 62 | 233 | 3.46e-12 | 66.6 |
Find 62 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAATTCTCATTCAATTT+TGG | 0.100198 | 1.1:+48966905 | None:intergenic |
| CACTTATTTGCCGAAGTTTA+AGG | 0.149063 | 1.1:-48967107 | MS.gene073761:CDS |
| CTTTCAGATATATATGAAAA+AGG | 0.205406 | 1.1:-48966737 | MS.gene073761:CDS |
| TGGAATTGGTGCTTTGCTTT+TGG | 0.223055 | 1.1:-48967434 | MS.gene073761:CDS |
| TTCTTCAAGTAAGAAGAATT+TGG | 0.288457 | 1.1:-48967080 | MS.gene073761:CDS |
| AATTTGGTTTATGTTAATAT+TGG | 0.311724 | 1.1:-48967064 | MS.gene073761:CDS |
| TTGAAGTCTTTCTTATCAAT+TGG | 0.335179 | 1.1:+48967004 | None:intergenic |
| GAACGTATTCTTAAACCTAA+TGG | 0.351263 | 1.1:-48967454 | MS.gene073761:CDS |
| GCAGTCATGATCATAGGTTT+TGG | 0.351442 | 1.1:+48967867 | None:intergenic |
| ATTCTTAAACCTAATGGAAT+TGG | 0.357672 | 1.1:-48967448 | MS.gene073761:CDS |
| GAAGCAGAAACTCTTTGTAT+TGG | 0.379951 | 1.1:-48967670 | MS.gene073761:CDS |
| AGAGGACAAAGAGTTTGATT+TGG | 0.392534 | 1.1:-48966843 | MS.gene073761:CDS |
| TTTCGCAGCAACGCTGAAAT+CGG | 0.404649 | 1.1:+48967346 | None:intergenic |
| GATGATGCAGTCATGATCAT+AGG | 0.409066 | 1.1:+48967861 | None:intergenic |
| AGCAAAGCACCAATTCCATT+AGG | 0.409665 | 1.1:+48967439 | None:intergenic |
| ATGGTTAAGATGAATATTGC+AGG | 0.417602 | 1.1:-48966779 | MS.gene073761:CDS |
| TAAGAATACGTTCAACTTCA+AGG | 0.424190 | 1.1:+48967464 | None:intergenic |
| AGTGTTGTCAAAGAACTAAA+AGG | 0.425355 | 1.1:-48967712 | MS.gene073761:CDS |
| GAAGAAACATCCTTAAACTT+CGG | 0.466732 | 1.1:+48967097 | None:intergenic |
| AAGAGTTTGATTTGGTTGCT+TGG | 0.475847 | 1.1:-48966835 | MS.gene073761:CDS |
| GTTAGTCTTGTCAATAATCA+TGG | 0.484833 | 1.1:-48967307 | MS.gene073761:CDS |
| AGTGAAGGAAACACCATATG+AGG | 0.490263 | 1.1:-48967137 | MS.gene073761:CDS |
| GTTTCCACACTGAAGAAACT+TGG | 0.492186 | 1.1:-48967628 | MS.gene073761:CDS |
| GTGAAGATGAGGTTGGTGAT+TGG | 0.495931 | 1.1:-48967042 | MS.gene073761:CDS |
| ACTTCAAGGACAACCAATGC+AGG | 0.500181 | 1.1:+48967478 | None:intergenic |
| AGCTCTTTGTTCTATCATCA+AGG | 0.501727 | 1.1:-48967226 | MS.gene073761:CDS |
| TTATCAATTGGATAAGATTG+TGG | 0.505859 | 1.1:+48967016 | None:intergenic |
| GTGGAGATGGTGAAAGAGAG+AGG | 0.514391 | 1.1:-48966670 | MS.gene073761:CDS |
| AAAAGTTGCTGTCCCTGCAT+TGG | 0.518228 | 1.1:-48967491 | MS.gene073761:CDS |
| CTCTGGAACAAATAAGAACC+TGG | 0.520480 | 1.1:+48967805 | None:intergenic |
| TACAAGGGTCTTAAAAGCAA+TGG | 0.520701 | 1.1:-48966635 | MS.gene073761:CDS |
| ATGGTGTCTTTGTTCATCAA+TGG | 0.529795 | 1.1:-48966616 | MS.gene073761:CDS |
| TGGTGAAAGAGAGAGGTGCA+TGG | 0.537641 | 1.1:-48966663 | MS.gene073761:CDS |
| ATTATGTTGTCGCACGTGAA+AGG | 0.538596 | 1.1:-48966953 | MS.gene073761:CDS |
| TTTCTTAGCTGCTCAGAGAG+TGG | 0.552627 | 1.1:-48966689 | MS.gene073761:CDS |
| AGCTGCTCAGAGAGTGGAGA+TGG | 0.561662 | 1.1:-48966683 | MS.gene073761:CDS |
| AATCGAGCCGCCACTAGTGA+AGG | 0.565391 | 1.1:-48967152 | MS.gene073761:CDS |
| GAAACCAAGTTTCTTCAGTG+TGG | 0.567718 | 1.1:+48967624 | None:intergenic |
| GGTGTTTCCTTCACTAGTGG+CGG | 0.572347 | 1.1:+48967145 | None:intergenic |
| AACTGTTTAAGACTCTACTC+TGG | 0.577491 | 1.1:+48967788 | None:intergenic |
| TATGGTGTTTCCTTCACTAG+TGG | 0.578954 | 1.1:+48967142 | None:intergenic |
| TCTTCACTCACTACCTACCA+TGG | 0.580333 | 1.1:-48967899 | MS.gene073761:CDS |
| GAGGTGCATGGATATTTACA+AGG | 0.586229 | 1.1:-48966651 | MS.gene073761:CDS |
| GGATGAAACTAAGGTTAATG+TGG | 0.587970 | 1.1:-48966882 | MS.gene073761:CDS |
| TCAAACCTCACTTTAACACC+AGG | 0.590536 | 1.1:-48967823 | MS.gene073761:CDS |
| TGAGTGAAGAAGAGAAACGA+TGG | 0.592739 | 1.1:+48967912 | None:intergenic |
| AATATTGGTGAAGATGAGGT+TGG | 0.595070 | 1.1:-48967049 | MS.gene073761:CDS |
| AAGAACCTGGTGTTAAAGTG+AGG | 0.599372 | 1.1:+48967818 | None:intergenic |
| GATGAAACTAAGGTTAATGT+GGG | 0.601801 | 1.1:-48966881 | MS.gene073761:CDS |
| GTGTCTTTGTTCATCAATGG+TGG | 0.604469 | 1.1:-48966613 | MS.gene073761:CDS |
| TTGGGAGCTAAATCCATGGT+AGG | 0.606124 | 1.1:+48967886 | None:intergenic |
| TAATGTGGGCGATGCAGATG+TGG | 0.610999 | 1.1:-48966867 | MS.gene073761:CDS |
| TGTTAATATTGGTGAAGATG+AGG | 0.620594 | 1.1:-48967053 | MS.gene073761:CDS |
| GGGCGATGCAGATGTGGAAG+AGG | 0.632072 | 1.1:-48966861 | MS.gene073761:CDS |
| TCAAAGAACTAAAAGGTGAA+CGG | 0.636645 | 1.1:-48967705 | MS.gene073761:CDS |
| TGCTTGGTTTAAAGAAACTG+TGG | 0.641228 | 1.1:-48966819 | MS.gene073761:CDS |
| CTTCAAGGACAACCAATGCA+GGG | 0.669621 | 1.1:+48967479 | None:intergenic |
| AGAAACGATGGAAATAGCAG+AGG | 0.677899 | 1.1:+48967924 | None:intergenic |
| AGGTGCATGGATATTTACAA+GGG | 0.710220 | 1.1:-48966650 | MS.gene073761:CDS |
| CAAAGTCAAACGATTCATCG+TGG | 0.713449 | 1.1:+48967533 | None:intergenic |
| TGTTGAATGAGAAAACACGA+TGG | 0.736414 | 1.1:+48967587 | None:intergenic |
| TGATAAACAAATGTAACACG+CGG | 0.737525 | 1.1:+48966929 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCAGATATATATGAAAAA+GGG | - | chr1.1:48967802-48967821 | MS.gene073761:CDS | 15.0% |
| !!! | AATTTGGTTTATGTTAATAT+TGG | - | chr1.1:48967474-48967493 | MS.gene073761:CDS | 15.0% |
| !! | CTTTCAGATATATATGAAAA+AGG | - | chr1.1:48967801-48967820 | MS.gene073761:CDS | 20.0% |
| !!! | AAAATTGAATGAGAATTTTG+AGG | - | chr1.1:48967635-48967654 | MS.gene073761:CDS | 20.0% |
| !!! | ATTTTTTCTTGAAAACAACA+AGG | + | chr1.1:48967257-48967276 | None:intergenic | 20.0% |
| !!! | TCAAAATTCTCATTCAATTT+TGG | + | chr1.1:48967636-48967655 | None:intergenic | 20.0% |
| ! | ATTCTTAAACCTAATGGAAT+TGG | - | chr1.1:48967090-48967109 | MS.gene073761:CDS | 25.0% |
| ! | TTATCAATTGGATAAGATTG+TGG | + | chr1.1:48967525-48967544 | None:intergenic | 25.0% |
| ! | TTCTTCAAGTAAGAAGAATT+TGG | - | chr1.1:48967458-48967477 | MS.gene073761:CDS | 25.0% |
| ! | TTGAAGTCTTTCTTATCAAT+TGG | + | chr1.1:48967537-48967556 | None:intergenic | 25.0% |
| ! | TTGCAAAAAAGAAATCAACT+TGG | - | chr1.1:48966798-48966817 | MS.gene073761:CDS | 25.0% |
| !! | TAGAGTCTTAAACAGTTTTT+GGG | - | chr1.1:48966756-48966775 | MS.gene073761:CDS | 25.0% |
| !! | TTAACACTTTTACAATCTTC+TGG | + | chr1.1:48967342-48967361 | None:intergenic | 25.0% |
| !!! | TAAGTGATTTTTTCCTCATA+TGG | + | chr1.1:48967417-48967436 | None:intergenic | 25.0% |
| !!! | TTTGCTTTTGGATTTTGATA+GGG | - | chr1.1:48967116-48967135 | MS.gene073761:CDS | 25.0% |
| !!! | TTTGTTTTGTCTAAGGATTT+GGG | - | chr1.1:48967024-48967043 | MS.gene073761:CDS | 25.0% |
| AGTGTTGTCAAAGAACTAAA+AGG | - | chr1.1:48966826-48966845 | MS.gene073761:CDS | 30.0% | |
| ATGGTTAAGATGAATATTGC+AGG | - | chr1.1:48967759-48967778 | MS.gene073761:CDS | 30.0% | |
| GAACGTATTCTTAAACCTAA+TGG | - | chr1.1:48967084-48967103 | MS.gene073761:CDS | 30.0% | |
| GAAGAAACATCCTTAAACTT+CGG | + | chr1.1:48967444-48967463 | None:intergenic | 30.0% | |
| GATGAAACTAAGGTTAATGT+GGG | - | chr1.1:48967657-48967676 | MS.gene073761:CDS | 30.0% | |
| TAAGAATACGTTCAACTTCA+AGG | + | chr1.1:48967077-48967096 | None:intergenic | 30.0% | |
| TCAAAGAACTAAAAGGTGAA+CGG | - | chr1.1:48966833-48966852 | MS.gene073761:CDS | 30.0% | |
| TGATAAACAAATGTAACACG+CGG | + | chr1.1:48967612-48967631 | None:intergenic | 30.0% | |
| TGTTAATATTGGTGAAGATG+AGG | - | chr1.1:48967485-48967504 | MS.gene073761:CDS | 30.0% | |
| ! | AGAGTCTTAAACAGTTTTTG+GGG | - | chr1.1:48966757-48966776 | MS.gene073761:CDS | 30.0% |
| ! | GTAGAGTCTTAAACAGTTTT+TGG | - | chr1.1:48966755-48966774 | MS.gene073761:CDS | 30.0% |
| !! | CTTTGCTTTTGGATTTTGAT+AGG | - | chr1.1:48967115-48967134 | MS.gene073761:CDS | 30.0% |
| !! | GAATTTTGAGGATGAAACTA+AGG | - | chr1.1:48967647-48967666 | MS.gene073761:CDS | 30.0% |
| !! | GTTAGTCTTGTCAATAATCA+TGG | - | chr1.1:48967231-48967250 | MS.gene073761:CDS | 30.0% |
| !!! | CTTTGTTTTGTCTAAGGATT+TGG | - | chr1.1:48967023-48967042 | MS.gene073761:CDS | 30.0% |
| !!! | GTTTGACTTTGTTTTGTCTA+AGG | - | chr1.1:48967017-48967036 | MS.gene073761:CDS | 30.0% |
| AATATTGGTGAAGATGAGGT+TGG | - | chr1.1:48967489-48967508 | MS.gene073761:CDS | 35.0% | |
| AGAGGACAAAGAGTTTGATT+TGG | - | chr1.1:48967695-48967714 | MS.gene073761:CDS | 35.0% | |
| AGCTCTTTGTTCTATCATCA+AGG | - | chr1.1:48967312-48967331 | MS.gene073761:CDS | 35.0% | |
| AGGTGCATGGATATTTACAA+GGG | - | chr1.1:48967888-48967907 | MS.gene073761:CDS | 35.0% | |
| ATGGTGTCTTTGTTCATCAA+TGG | - | chr1.1:48967922-48967941 | MS.gene073761:CDS | 35.0% | |
| CACTTATTTGCCGAAGTTTA+AGG | - | chr1.1:48967431-48967450 | MS.gene073761:CDS | 35.0% | |
| GAAGCAGAAACTCTTTGTAT+TGG | - | chr1.1:48966868-48966887 | MS.gene073761:CDS | 35.0% | |
| GGATGAAACTAAGGTTAATG+TGG | - | chr1.1:48967656-48967675 | MS.gene073761:CDS | 35.0% | |
| TACAAGGGTCTTAAAAGCAA+TGG | - | chr1.1:48967903-48967922 | MS.gene073761:CDS | 35.0% | |
| TGCTTGGTTTAAAGAAACTG+TGG | - | chr1.1:48967719-48967738 | MS.gene073761:CDS | 35.0% | |
| TGTTGAATGAGAAAACACGA+TGG | + | chr1.1:48966954-48966973 | None:intergenic | 35.0% | |
| ! | AACTGTTTAAGACTCTACTC+TGG | + | chr1.1:48966753-48966772 | None:intergenic | 35.0% |
| ! | CAGTCATGATCATAGGTTTT+GGG | + | chr1.1:48966673-48966692 | None:intergenic | 35.0% |
| !! | AAGAGTTTGATTTGGTTGCT+TGG | - | chr1.1:48967703-48967722 | MS.gene073761:CDS | 35.0% |
| !!! | GGAGAATGCTGATTTTGTTA+TGG | - | chr1.1:48967740-48967759 | MS.gene073761:CDS | 35.0% |
| AGAAACGATGGAAATAGCAG+AGG | + | chr1.1:48966617-48966636 | None:intergenic | 40.0% | |
| AGTGAAGGAAACACCATATG+AGG | - | chr1.1:48967401-48967420 | MS.gene073761:CDS | 40.0% | |
| ATTATGTTGTCGCACGTGAA+AGG | - | chr1.1:48967585-48967604 | MS.gene073761:CDS | 40.0% | |
| CAAAGTCAAACGATTCATCG+TGG | + | chr1.1:48967008-48967027 | None:intergenic | 40.0% | |
| GAAACCAAGTTTCTTCAGTG+TGG | + | chr1.1:48966917-48966936 | None:intergenic | 40.0% | |
| GAGGTGCATGGATATTTACA+AGG | - | chr1.1:48967887-48967906 | MS.gene073761:CDS | 40.0% | |
| GATGATGCAGTCATGATCAT+AGG | + | chr1.1:48966680-48966699 | None:intergenic | 40.0% | |
| GTGTCTTTGTTCATCAATGG+TGG | - | chr1.1:48967925-48967944 | MS.gene073761:CDS | 40.0% | |
| GTTTCCACACTGAAGAAACT+TGG | - | chr1.1:48966910-48966929 | MS.gene073761:CDS | 40.0% | |
| TATGGTGTTTCCTTCACTAG+TGG | + | chr1.1:48967399-48967418 | None:intergenic | 40.0% | |
| TCAAACCTCACTTTAACACC+AGG | - | chr1.1:48966715-48966734 | MS.gene073761:CDS | 40.0% | |
| TGAGTGAAGAAGAGAAACGA+TGG | + | chr1.1:48966629-48966648 | None:intergenic | 40.0% | |
| ! | AAGAACCTGGTGTTAAAGTG+AGG | + | chr1.1:48966723-48966742 | None:intergenic | 40.0% |
| ! | AGCAAAGCACCAATTCCATT+AGG | + | chr1.1:48967102-48967121 | None:intergenic | 40.0% |
| ! | GCAGTCATGATCATAGGTTT+TGG | + | chr1.1:48966674-48966693 | None:intergenic | 40.0% |
| !! | CTCTGGAACAAATAAGAACC+TGG | + | chr1.1:48966736-48966755 | None:intergenic | 40.0% |
| !! | TGGAATTGGTGCTTTGCTTT+TGG | - | chr1.1:48967104-48967123 | MS.gene073761:CDS | 40.0% |
| AAAAGTTGCTGTCCCTGCAT+TGG | - | chr1.1:48967047-48967066 | MS.gene073761:CDS | 45.0% | |
| ACTTCAAGGACAACCAATGC+AGG | + | chr1.1:48967063-48967082 | None:intergenic | 45.0% | |
| CTTCAAGGACAACCAATGCA+GGG | + | chr1.1:48967062-48967081 | None:intergenic | 45.0% | |
| TCTTCACTCACTACCTACCA+TGG | - | chr1.1:48966639-48966658 | MS.gene073761:CDS | 45.0% | |
| TTGGGAGCTAAATCCATGGT+AGG | + | chr1.1:48966655-48966674 | None:intergenic | 45.0% | |
| TTTCGCAGCAACGCTGAAAT+CGG | + | chr1.1:48967195-48967214 | None:intergenic | 45.0% | |
| TTTCTTAGCTGCTCAGAGAG+TGG | - | chr1.1:48967849-48967868 | MS.gene073761:CDS | 45.0% | |
| !! | GTGAAGATGAGGTTGGTGAT+TGG | - | chr1.1:48967496-48967515 | MS.gene073761:CDS | 45.0% |
| !!! | GGTTTTGGGAGCTAAATCCA+TGG | + | chr1.1:48966659-48966678 | None:intergenic | 45.0% |
| GGTGTTTCCTTCACTAGTGG+CGG | + | chr1.1:48967396-48967415 | None:intergenic | 50.0% | |
| GTGGAGATGGTGAAAGAGAG+AGG | - | chr1.1:48967868-48967887 | MS.gene073761:CDS | 50.0% | |
| TAATGTGGGCGATGCAGATG+TGG | - | chr1.1:48967671-48967690 | MS.gene073761:CDS | 50.0% | |
| ! | TGGTGAAAGAGAGAGGTGCA+TGG | - | chr1.1:48967875-48967894 | MS.gene073761:CDS | 50.0% |
| AATCGAGCCGCCACTAGTGA+AGG | - | chr1.1:48967386-48967405 | MS.gene073761:CDS | 55.0% | |
| AGCTGCTCAGAGAGTGGAGA+TGG | - | chr1.1:48967855-48967874 | MS.gene073761:CDS | 55.0% | |
| GGGCGATGCAGATGTGGAAG+AGG | - | chr1.1:48967677-48967696 | MS.gene073761:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 48966577 | 48967983 | 48966577 | ID=MS.gene073761 |
| chr1.1 | mRNA | 48966577 | 48967983 | 48966577 | ID=MS.gene073761.t1;Parent=MS.gene073761 |
| chr1.1 | exon | 48966577 | 48967983 | 48966577 | ID=MS.gene073761.t1.exon1;Parent=MS.gene073761.t1 |
| chr1.1 | CDS | 48966577 | 48967983 | 48966577 | ID=cds.MS.gene073761.t1;Parent=MS.gene073761.t1 |
| Gene Sequence |
| Protein sequence |
