Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074129.t1 | RHN56897.1 | 71.1 | 90 | 26 | 0 | 1 | 90 | 87 | 176 | 3.60E-30 | 140.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074129.t1 | A0A072TKK1 | 71.1 | 90 | 26 | 0 | 1 | 90 | 311 | 400 | 2.6e-30 | 140.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056050 | MS.gene074129 | 0.804562 | 2.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074129.t1 | MTR_5g077900 | 71.111 | 90 | 26 | 0 | 1 | 90 | 137 | 226 | 3.47e-44 | 142 |
| MS.gene074129.t1 | MTR_0002s1270 | 71.111 | 90 | 26 | 0 | 1 | 90 | 311 | 400 | 1.11e-42 | 143 |
| MS.gene074129.t1 | MTR_7g046380 | 70.513 | 78 | 23 | 0 | 1 | 78 | 319 | 396 | 3.00e-34 | 120 |
| MS.gene074129.t1 | MTR_0057s0200 | 78.947 | 57 | 12 | 0 | 32 | 88 | 1 | 57 | 4.28e-30 | 101 |
| MS.gene074129.t1 | MTR_4g066320 | 54.118 | 85 | 38 | 1 | 6 | 89 | 36 | 120 | 1.43e-25 | 92.0 |
| MS.gene074129.t1 | MTR_2g086200 | 80.000 | 50 | 10 | 0 | 41 | 90 | 185 | 234 | 2.38e-21 | 84.0 |
| MS.gene074129.t1 | MTR_3g028430 | 60.377 | 53 | 12 | 1 | 37 | 89 | 15 | 58 | 7.31e-16 | 65.5 |
| MS.gene074129.t1 | MTR_2g092960 | 43.373 | 83 | 46 | 1 | 5 | 86 | 1133 | 1215 | 1.81e-14 | 67.4 |
| MS.gene074129.t1 | MTR_0462s0020 | 38.372 | 86 | 51 | 1 | 3 | 86 | 206 | 291 | 1.86e-14 | 66.6 |
| MS.gene074129.t1 | MTR_2g037270 | 36.782 | 87 | 53 | 1 | 5 | 89 | 602 | 688 | 3.41e-13 | 63.5 |
| MS.gene074129.t1 | MTR_8g017000 | 36.471 | 85 | 52 | 1 | 5 | 87 | 602 | 686 | 8.20e-13 | 62.4 |
| MS.gene074129.t1 | MTR_4g066300 | 67.647 | 34 | 11 | 0 | 28 | 61 | 5 | 38 | 3.94e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCCACAATCAGTTGTGTT+AGG | 0.390652 | 6.1:+6619717 | None:intergenic |
| TCTTGAAGTATATGAAACTT+AGG | 0.430557 | 6.1:-6619453 | None:intergenic |
| GTATGCTTTGCGAAACGATT+GGG | 0.447565 | 6.1:-6619486 | MS.gene074129:CDS |
| CTGCTAATATTACTACCTCT+TGG | 0.458064 | 6.1:-6619662 | MS.gene074129:CDS |
| GGTTCGTTGGCTTCGTCCTA+AGG | 0.471145 | 6.1:-6619835 | MS.gene074129:intron |
| CAACCTAACACAACTGATTG+TGG | 0.514100 | 6.1:-6619720 | MS.gene074129:CDS |
| TATTACTACCTCTTGGATGA+AGG | 0.535487 | 6.1:-6619655 | MS.gene074129:intron |
| TGTATGCTTTGCGAAACGAT+TGG | 0.544311 | 6.1:-6619487 | MS.gene074129:CDS |
| GAGGTTCATCAATTAGCAAA+CGG | 0.566698 | 6.1:-6619870 | MS.gene074129:CDS |
| ATGGTATACCTTCATCCAAG+AGG | 0.683941 | 6.1:+6619647 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAGTAAAATAACAATA+AGG | + | chr6.1:6619873-6619892 | None:intergenic | 10.0% |
| !! | TAAAAATTAAATTCATTTGT+AGG | - | chr6.1:6619688-6619707 | MS.gene074129:CDS | 10.0% |
| !!! | TAATCTATTTTACAAACTTT+AGG | - | chr6.1:6619893-6619912 | MS.gene074129:intron | 15.0% |
| !! | TATGTCTATCTATCTTAATT+TGG | + | chr6.1:6619849-6619868 | None:intergenic | 20.0% |
| ! | ATATATATGGTGCAAACATA+TGG | + | chr6.1:6619814-6619833 | None:intergenic | 25.0% |
| ! | ATTAAATTCATTTGTAGGCA+AGG | - | chr6.1:6619693-6619712 | MS.gene074129:CDS | 25.0% |
| AGCAAACGGTATTAAAAAGA+AGG | - | chr6.1:6619583-6619602 | MS.gene074129:intron | 30.0% | |
| GTATTAAAAAGAAGGTTCGT+TGG | - | chr6.1:6619591-6619610 | MS.gene074129:intron | 30.0% | |
| !!! | ATTTTTTTAACCTAGTGCTC+TGG | - | chr6.1:6619547-6619566 | MS.gene074129:intron | 30.0% |
| !!! | GTTCTATGTTTTAGTGTTGT+AGG | + | chr6.1:6619516-6619535 | None:intergenic | 30.0% |
| ATCTTGTCTACACATACCTT+AGG | + | chr6.1:6619623-6619642 | None:intergenic | 35.0% | |
| CTGCTAATATTACTACCTCT+TGG | - | chr6.1:6619777-6619796 | MS.gene074129:intron | 35.0% | |
| GAGGTTCATCAATTAGCAAA+CGG | - | chr6.1:6619569-6619588 | MS.gene074129:intron | 35.0% | |
| TAAGGTATGTGTAGACAAGA+TGG | - | chr6.1:6619622-6619641 | MS.gene074129:intron | 35.0% | |
| TATCCACAATCAGTTGTGTT+AGG | + | chr6.1:6619725-6619744 | None:intergenic | 35.0% | |
| TATTACTACCTCTTGGATGA+AGG | - | chr6.1:6619784-6619803 | MS.gene074129:intron | 35.0% | |
| ATGGTATACCTTCATCCAAG+AGG | + | chr6.1:6619795-6619814 | None:intergenic | 40.0% | |
| CAACCTAACACAACTGATTG+TGG | - | chr6.1:6619719-6619738 | MS.gene074129:CDS | 40.0% | |
| GTATGCTTTGCGAAACGATT+GGG | - | chr6.1:6619953-6619972 | MS.gene074129:intron | 40.0% | |
| TGTATGCTTTGCGAAACGAT+TGG | - | chr6.1:6619952-6619971 | MS.gene074129:intron | 40.0% | |
| ! | TTTTTAACCTAGTGCTCTGG+AGG | - | chr6.1:6619550-6619569 | MS.gene074129:intron | 40.0% |
| !! | GAAATATATATATATATATA+TGG | + | chr6.1:6619827-6619846 | None:intergenic | 5.0% |
| ! | TGATGAACCTCCAGAGCACT+AGG | + | chr6.1:6619560-6619579 | None:intergenic | 50.0% |
| !! | GGTTCGTTGGCTTCGTCCTA+AGG | - | chr6.1:6619604-6619623 | MS.gene074129:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 6619454 | 6620007 | 6619454 | ID=MS.gene074129 |
| chr6.1 | mRNA | 6619454 | 6620007 | 6619454 | ID=MS.gene074129.t1;Parent=MS.gene074129 |
| chr6.1 | exon | 6619988 | 6620007 | 6619988 | ID=MS.gene074129.t1.exon1;Parent=MS.gene074129.t1 |
| chr6.1 | CDS | 6619988 | 6620007 | 6619988 | ID=cds.MS.gene074129.t1;Parent=MS.gene074129.t1 |
| chr6.1 | exon | 6619836 | 6619899 | 6619836 | ID=MS.gene074129.t1.exon2;Parent=MS.gene074129.t1 |
| chr6.1 | CDS | 6619836 | 6619899 | 6619836 | ID=cds.MS.gene074129.t1;Parent=MS.gene074129.t1 |
| chr6.1 | exon | 6619656 | 6619751 | 6619656 | ID=MS.gene074129.t1.exon3;Parent=MS.gene074129.t1 |
| chr6.1 | CDS | 6619656 | 6619751 | 6619656 | ID=cds.MS.gene074129.t1;Parent=MS.gene074129.t1 |
| chr6.1 | exon | 6619454 | 6619546 | 6619454 | ID=MS.gene074129.t1.exon4;Parent=MS.gene074129.t1 |
| chr6.1 | CDS | 6619454 | 6619546 | 6619454 | ID=cds.MS.gene074129.t1;Parent=MS.gene074129.t1 |
| Gene Sequence |
| Protein sequence |
