AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074488

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074488.t1 MTR_5g099010 95.377 584 22 2 1 584 2 580 0.0 1159
MS.gene074488.t1 MTR_3g115220 65.833 480 155 3 40 510 20 499 0.0 677
MS.gene074488.t1 MTR_4g100810 60.140 429 167 3 94 519 79 506 0.0 546
MS.gene074488.t1 MTR_5g012770 55.180 473 187 6 39 504 41 495 7.50e-180 518
MS.gene074488.t1 MTR_1g031590 36.863 510 289 12 20 504 46 547 6.68e-100 314
MS.gene074488.t1 MTR_5g042200 45.316 395 196 8 126 504 88 478 2.18e-98 308
MS.gene074488.t1 MTR_4g084570 36.595 511 291 12 19 504 46 548 4.89e-97 307
MS.gene074488.t1 MTR_4g082310 42.459 431 222 10 112 522 80 504 6.16e-97 305
MS.gene074488.t1 MTR_5g064620 42.202 436 219 13 95 505 86 513 1.41e-96 305
MS.gene074488.t1 MTR_3g099480 40.217 460 246 11 18 458 27 476 2.71e-95 302
MS.gene074488.t1 MTR_3g096020 38.230 531 283 13 6 513 85 593 5.78e-94 300
MS.gene074488.t1 MTR_6g016015 42.347 392 209 7 126 502 118 507 4.47e-93 295
MS.gene074488.t1 MTR_2g016470 37.013 462 233 12 95 504 160 615 3.86e-92 296
MS.gene074488.t1 MTR_4g019210 38.462 455 255 14 74 513 187 631 5.92e-92 296
MS.gene074488.t1 MTR_3g005340 38.979 431 235 9 96 505 123 546 1.05e-91 293
MS.gene074488.t1 MTR_5g059260 43.869 367 184 7 126 473 160 523 1.84e-89 288
MS.gene074488.t1 MTR_5g081690 35.542 498 293 13 20 504 29 511 2.31e-89 286
MS.gene074488.t1 MTR_4g125460 37.705 427 249 9 94 508 62 483 5.18e-89 283
MS.gene074488.t1 MTR_4g110030 41.944 391 212 7 126 502 119 508 2.66e-88 283
MS.gene074488.t1 MTR_4g125460 37.995 429 245 11 94 508 201 622 3.77e-87 283
MS.gene074488.t1 MTR_2g059350 37.986 437 238 10 94 502 76 507 1.45e-86 278
MS.gene074488.t1 MTR_3g086570 39.950 398 218 11 126 504 153 548 1.04e-85 277
MS.gene074488.t1 MTR_5g032100 36.504 452 237 13 94 504 60 502 1.37e-80 262
MS.gene074488.t1 MTR_4g132590 38.442 398 194 8 126 474 68 463 1.14e-78 257
MS.gene074488.t1 MTR_3g100310 33.855 511 268 17 47 504 23 516 1.49e-78 257
MS.gene074488.t1 MTR_4g132590 38.442 398 194 8 126 474 167 562 1.17e-77 257
MS.gene074488.t1 MTR_3g026650 35.945 434 259 9 88 509 136 562 6.52e-77 254
MS.gene074488.t1 MTR_1g102030 35.648 432 254 10 94 507 61 486 1.03e-74 247
MS.gene074488.t1 MTR_4g023030 35.963 431 257 9 88 506 196 619 1.47e-70 239
MS.gene074488.t1 MTR_3g026650 37.640 356 208 5 88 434 136 486 1.22e-65 222
MS.gene074488.t1 MTR_3g086570 40.647 278 148 7 126 386 153 430 6.38e-61 208
MS.gene074488.t1 MTR_1g101990 37.676 284 161 8 233 507 6 282 2.71e-53 184
MS.gene074488.t1 MTR_7g017330 31.152 382 231 11 39 398 19 390 6.25e-42 160
MS.gene074488.t1 MTR_4g010990 30.178 338 198 9 126 447 78 393 2.90e-38 147
MS.gene074488.t1 MTR_3g026650 37.387 222 135 3 88 307 136 355 3.42e-38 145
MS.gene074488.t1 MTR_7g064210 48.684 152 64 3 251 390 39 188 1.48e-34 135
MS.gene074488.t1 MTR_4g010990 29.538 325 191 9 139 447 2 304 1.84e-34 134
MS.gene074488.t1 MTR_3g092590 28.682 387 249 11 36 402 7 386 8.34e-34 137
MS.gene074488.t1 MTR_3g092590 28.831 385 251 11 36 402 7 386 8.40e-34 136
MS.gene074488.t1 MTR_1g007390 32.472 271 162 8 151 402 124 392 2.40e-29 123
MS.gene074488.t1 MTR_2g038320 24.518 363 222 10 126 470 95 423 1.14e-20 95.1
MS.gene074488.t1 MTR_4g008200 26.113 337 204 12 124 449 36 338 2.80e-19 90.1
MS.gene074488.t1 MTR_4g008200 26.113 337 204 12 124 449 87 389 5.20e-19 90.1
MS.gene074488.t1 MTR_1g101985 40.385 104 60 1 94 197 61 162 5.54e-19 85.5
MS.gene074488.t1 MTR_1g095680 43.182 88 39 2 245 321 7 94 4.01e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074488.t1 AT1G76270 58.645 561 220 5 33 584 15 572 0.0 677
MS.gene074488.t1 AT4G38390 63.158 494 176 3 40 530 42 532 0.0 670
MS.gene074488.t1 AT1G20550 57.793 571 218 7 29 584 2 564 0.0 663
MS.gene074488.t1 AT4G16650 62.708 421 154 2 94 512 93 512 0.0 538
MS.gene074488.t1 AT4G16650 62.708 421 154 2 94 512 93 512 0.0 538
MS.gene074488.t1 AT2G01480 44.321 361 183 7 126 469 157 516 2.86e-97 308
MS.gene074488.t1 AT2G01480 44.633 354 179 6 126 462 157 510 1.12e-96 305
MS.gene074488.t1 AT1G22460 43.765 409 201 10 126 511 163 565 1.05e-95 303
MS.gene074488.t1 AT5G64600 37.087 515 270 13 28 505 19 516 3.54e-95 301
MS.gene074488.t1 AT1G14970 40.955 398 214 9 126 504 12 407 1.48e-92 291
MS.gene074488.t1 AT1G14970 40.955 398 214 9 126 504 38 433 3.25e-92 291
MS.gene074488.t1 AT1G35510 36.106 529 291 14 21 513 49 566 3.34e-92 295
MS.gene074488.t1 AT1G38065 42.268 388 201 7 109 474 17 403 3.59e-92 291
MS.gene074488.t1 AT1G14020 42.640 394 210 7 127 506 95 486 9.89e-92 291
MS.gene074488.t1 AT1G38065 43.716 366 188 6 126 474 29 393 1.63e-91 289
MS.gene074488.t1 AT1G14970 40.955 398 214 9 126 504 158 553 7.02e-91 291
MS.gene074488.t1 AT2G03280 42.967 391 206 8 127 502 92 480 3.10e-90 287
MS.gene074488.t1 AT5G35570 40.654 428 235 11 95 509 225 646 3.37e-90 291
MS.gene074488.t1 AT1G38131 43.716 366 188 6 126 474 158 522 7.75e-90 289
MS.gene074488.t1 AT2G37980 39.450 436 231 12 94 509 199 621 4.67e-89 288
MS.gene074488.t1 AT5G15740 41.117 394 211 7 126 502 109 498 9.99e-89 284
MS.gene074488.t1 AT3G54100 34.630 514 293 14 20 509 123 617 2.70e-87 284
MS.gene074488.t1 AT3G02250 40.921 391 216 7 126 502 109 498 7.70e-87 279
MS.gene074488.t1 AT1G29200 39.480 423 209 8 97 474 44 464 1.14e-85 275
MS.gene074488.t1 AT1G29200 39.480 423 209 8 97 474 44 464 1.14e-85 275
MS.gene074488.t1 AT2G03280 40.191 418 206 9 127 502 92 507 2.08e-84 272
MS.gene074488.t1 AT1G29200 39.480 423 209 8 97 474 160 580 2.26e-84 275
MS.gene074488.t1 AT3G26370 39.447 398 220 11 126 504 161 556 4.18e-84 273
MS.gene074488.t1 AT1G29200 41.071 392 184 8 128 474 1 390 7.83e-84 268
MS.gene074488.t1 AT5G65470 37.885 454 230 13 94 504 59 503 1.69e-83 270
MS.gene074488.t1 AT1G11990 40.872 367 199 6 126 474 193 559 1.08e-81 268
MS.gene074488.t1 AT1G62330 36.776 397 203 6 126 474 224 620 1.65e-81 269
MS.gene074488.t1 AT1G04910 36.277 419 255 7 94 504 61 475 4.36e-80 261
MS.gene074488.t1 AT4G24530 35.412 449 246 12 94 504 75 517 1.26e-79 260
MS.gene074488.t1 AT5G01100 31.407 519 303 15 38 518 120 623 7.68e-78 258
MS.gene074488.t1 AT1G11990 37.376 404 198 7 126 474 178 581 2.13e-75 251
MS.gene074488.t1 AT2G44500 35.714 420 260 6 94 505 146 563 5.13e-75 249
MS.gene074488.t1 AT5G63390 36.318 402 246 5 94 488 133 531 1.23e-74 248
MS.gene074488.t1 AT3G07900 36.131 429 258 8 93 510 156 579 1.41e-71 241
MS.gene074488.t1 AT1G52630 30.366 382 221 12 76 440 30 383 7.28e-43 159
MS.gene074488.t1 AT3G30300 31.500 400 228 14 22 399 18 393 1.87e-41 159
MS.gene074488.t1 AT3G30300 31.500 400 228 14 22 399 18 393 1.87e-41 159
MS.gene074488.t1 AT3G03810 34.769 325 177 9 97 402 77 385 1.77e-39 153
MS.gene074488.t1 AT3G03810 34.769 325 177 9 97 402 77 385 1.77e-39 153
MS.gene074488.t1 AT1G52630 31.962 316 179 9 139 440 2 295 5.11e-38 144
MS.gene074488.t1 AT3G26370 38.756 209 117 6 126 323 161 369 8.59e-38 144
MS.gene074488.t1 AT2G44500 33.846 260 165 3 94 347 146 404 7.38e-36 139

Find 154 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCATCGCGCTACATTTA+AGG 0.117490 5.4:-78279715 MS.gene074488:intron
GTTATTCTTGTTCATGAATA+AGG 0.127801 5.4:+78278774 None:intergenic
GCATTCTCTGGTTGCTATTA+TGG 0.145988 5.4:-78279461 MS.gene074488:CDS
TGAAGAGCATTTACAATAAA+AGG 0.154073 5.4:+78281647 None:intergenic
AATTGGATCATACATCTTTC+TGG 0.170645 5.4:-78280411 MS.gene074488:CDS
ACAAATTTCTCTCCCATATT+TGG 0.187446 5.4:+78280165 None:intergenic
CAAATTTACGGATTCGATTC+AGG 0.201483 5.4:-78279783 MS.gene074488:CDS
TGTAAAGCAGCTACCAAATA+TGG 0.220138 5.4:-78280178 MS.gene074488:CDS
CTTGGAACACGCACTGTATA+TGG 0.243424 5.4:+78280138 None:intergenic
ATGCTTAGAGCACTAGGTTT+TGG 0.277255 5.4:-78279223 MS.gene074488:CDS
CTACCATGCTCTCAAATTTA+CGG 0.290291 5.4:-78279795 MS.gene074488:CDS
CCATGTCTTCTTACTTTCTC+AGG 0.302140 5.4:+78279280 None:intergenic
TTATTCTTGTTCATGAATAA+GGG 0.305017 5.4:+78278775 None:intergenic
TTCTTTCTCTTCACTGATGA+TGG 0.313084 5.4:+78281821 None:intergenic
ACGCGACCCTCGTAGCATTT+GGG 0.325695 5.4:+78280105 None:intergenic
GAAAAGAACTTGGTGAAATT+AGG 0.326334 5.4:-78279421 MS.gene074488:CDS
TACTCTTGTTATTCCAGAAT+TGG 0.327689 5.4:-78280428 MS.gene074488:CDS
ACGCATTTGTATGTGGCTTC+TGG 0.336705 5.4:-78279193 MS.gene074488:CDS
TGTATTTCAGGAAAATGATT+TGG 0.337250 5.4:-78281540 MS.gene074488:intron
AACGCGACCCTCGTAGCATT+TGG 0.337594 5.4:+78280104 None:intergenic
CTACTTACAGGCATGATCTA+TGG 0.340612 5.4:-78281366 MS.gene074488:CDS
ATCTCGAGTTCGAACTTTCC+AGG 0.350555 5.4:-78278731 MS.gene074488:CDS
AAAGATGTATGATCCAATTC+TGG 0.354641 5.4:+78280415 None:intergenic
GTATCGATCCGGATGAGTTT+TGG 0.355722 5.4:+78281168 None:intergenic
AACTTTCCAGGTAGGCTTCA+TGG 0.360105 5.4:-78278719 MS.gene074488:CDS
GTCTGAATTAGATTATGATT+TGG 0.365270 5.4:-78278512 MS.gene074488:CDS
GAGACGACGGCGAAGGAAAC+TGG 0.366522 5.4:+78281748 None:intergenic
CTTAGCTACCAAGGAGGAAT+TGG 0.368531 5.4:-78279098 MS.gene074488:CDS
GTCTCCTTTGAATGGAAGTT+GGG 0.371214 5.4:+78279121 None:intergenic
CTTGGTCAGTACCCATTAGA+AGG 0.376857 5.4:+78278437 None:intergenic
AAGAGTAGCATTCAAAAGAT+AGG 0.384573 5.4:+78280444 None:intergenic
AGCATAAGACCAACTTCCTC+TGG 0.394026 5.4:+78279241 None:intergenic
CTCAATTATTTGTTGCCGAT+TGG 0.396141 5.4:-78280233 MS.gene074488:CDS
CCGGATATGCTTGCATTCTC+TGG 0.396269 5.4:-78279473 MS.gene074488:CDS
TCAAACGCATGAAGGCAATA+AGG 0.398688 5.4:-78278581 MS.gene074488:CDS
CCAGAGAATGCAAGCATATC+CGG 0.399860 5.4:+78279473 None:intergenic
GGCTTAAATCAACAGAGAAC+AGG 0.407017 5.4:-78281124 MS.gene074488:CDS
GCAATCGCCATTTCACATTC+CGG 0.408047 5.4:-78281489 MS.gene074488:intron
AGGCAAATGAAGCTACTTAC+AGG 0.416698 5.4:-78281378 MS.gene074488:CDS
TCAGGGAATGGGAAATTTGT+TGG 0.419141 5.4:-78279765 MS.gene074488:CDS
GTGGCTTCTGGAGAAATATA+TGG 0.419254 5.4:-78279181 MS.gene074488:CDS
ATCTACAAAAGCTGAGGTGC+AGG 0.420709 5.4:-78279823 MS.gene074488:CDS
CTGCCTGACCTCAACTCATT+AGG 0.423475 5.4:+78278691 None:intergenic
GAAGAGCATTTACAATAAAA+GGG 0.426667 5.4:+78281648 None:intergenic
CTACAGACTTTCTAATCTGC+TGG 0.431795 5.4:-78279852 MS.gene074488:CDS
AAATTTACGGATTCGATTCA+GGG 0.431803 5.4:-78279782 MS.gene074488:CDS
AGAGAGCTCTGAGGGGTGCT+AGG 0.437137 5.4:+78279146 None:intergenic
CTCTGGTTGCTATTATGGTG+GGG 0.437730 5.4:-78279456 MS.gene074488:CDS
CTGTTGATTTAAGCCTCCAC+TGG 0.440491 5.4:+78281132 None:intergenic
TACGGATTCGATTCAGGGAA+TGG 0.440840 5.4:-78279777 MS.gene074488:CDS
CTCTGTTGTAGGAGGTACTT+TGG 0.443197 5.4:-78278838 MS.gene074488:intron
GGTCTCCTTTGAATGGAAGT+TGG 0.455130 5.4:+78279120 None:intergenic
TCTCTGGTTGCTATTATGGT+GGG 0.455204 5.4:-78279457 MS.gene074488:CDS
ATTGCATTACACAGGTTGTT+CGG 0.456755 5.4:-78279889 MS.gene074488:intron
CGTGAAGAGAAAGACACAAA+TGG 0.458606 5.4:+78279073 None:intergenic
TTCTCTGCCTGTAGTAATGC+CGG 0.460147 5.4:-78281322 MS.gene074488:CDS
TGTTTCATATAGGTTTGAAC+CGG 0.461614 5.4:-78279492 MS.gene074488:intron
TAGCTAAGGTCTCCTTTGAA+TGG 0.465721 5.4:+78279113 None:intergenic
ACGGATTCGATTCAGGGAAT+GGG 0.465732 5.4:-78279776 MS.gene074488:CDS
TTCTCTGGTTGCTATTATGG+TGG 0.468408 5.4:-78279458 MS.gene074488:CDS
CATCTTTCTGGAAAGACAAC+AGG 0.468808 5.4:-78280399 MS.gene074488:intron
GTGAAGAGAAAGACACAAAT+GGG 0.470665 5.4:+78279074 None:intergenic
GCTCTCCCATGAAGCCTACC+TGG 0.479446 5.4:+78278713 None:intergenic
CAATTCCTCCTTGGTAGCTA+AGG 0.492942 5.4:+78279099 None:intergenic
CCAACTTCCTCTGGGGTAAG+TGG 0.493118 5.4:+78279250 None:intergenic
TTTGGACAATTATAGAAAGC+AGG 0.493179 5.4:-78278494 MS.gene074488:CDS
TCTGGTTGCTATTATGGTGG+GGG 0.493967 5.4:-78279455 MS.gene074488:CDS
GGTCTTATGCTTAGAGCACT+AGG 0.494580 5.4:-78279229 MS.gene074488:CDS
CTAAAATTCTAGCTGGAAGA+AGG 0.494617 5.4:-78278979 MS.gene074488:intron
ACTTTCCAGGTAGGCTTCAT+GGG 0.496124 5.4:-78278718 MS.gene074488:CDS
GGCTAATACCAAAACTCATC+CGG 0.498226 5.4:-78281176 MS.gene074488:CDS
CTCGTACTTCCTCAGCAGGC+TGG 0.501222 5.4:+78278539 None:intergenic
ACACGCTTCTTGACCAGCAC+TGG 0.503067 5.4:+78280078 None:intergenic
TGTGTCTTTCTCTTCACGCA+TGG 0.504664 5.4:-78279068 MS.gene074488:CDS
AAGGCAATAAGGTAGAAAAC+AGG 0.505309 5.4:-78278570 MS.gene074488:CDS
GAGCCTAATGAGTTGAGGTC+AGG 0.509212 5.4:-78278694 MS.gene074488:CDS
AAAGAGAAGGCAAACGGAGA+TGG 0.510159 5.4:+78281703 None:intergenic
CGCGACCCTCGTAGCATTTG+GGG 0.512807 5.4:+78280106 None:intergenic
GTAAAGCAGCTACCAAATAT+GGG 0.514532 5.4:-78280177 MS.gene074488:CDS
TTGATTACCGGAATGTGAAA+TGG 0.514677 5.4:+78281482 None:intergenic
AGAATACTTGCACTTCAGCT+TGG 0.514937 5.4:+78278419 None:intergenic
CTTCCCCAACTTCCATTCAA+AGG 0.524406 5.4:-78279125 MS.gene074488:CDS
AAGGGATGTTATAAACCAAT+CGG 0.525670 5.4:+78280218 None:intergenic
CATATACAGTGCGTGTTCCA+AGG 0.527953 5.4:-78280137 MS.gene074488:CDS
CCTGAGAAAGTAAGAAGACA+TGG 0.528230 5.4:-78279280 MS.gene074488:CDS
GTTGGGGAAGAGAGCTCTGA+GGG 0.529842 5.4:+78279138 None:intergenic
CACTCTCGTACTTCCTCAGC+AGG 0.530274 5.4:+78278535 None:intergenic
ATTGGTTTATAACATCCCTT+CGG 0.534376 5.4:-78280215 MS.gene074488:CDS
GAAAAGGAAAGAAAAGAACT+TGG 0.534475 5.4:-78279431 MS.gene074488:CDS
GCATAAGACCAACTTCCTCT+GGG 0.536685 5.4:+78279242 None:intergenic
GAAAAGAAAGAGAAGGCAAA+CGG 0.539093 5.4:+78281697 None:intergenic
CCTCAAAATCAAACGCATGA+AGG 0.539206 5.4:-78278589 MS.gene074488:CDS
CAACTCTTATGTCATTCCGA+AGG 0.542501 5.4:+78280199 None:intergenic
AACATGGCTAAAATTCTAGC+TGG 0.543477 5.4:-78278986 MS.gene074488:CDS
GTTGACACCGGCATTACTAC+AGG 0.543830 5.4:+78281315 None:intergenic
GCAGATAATTGATGCTGTTG+TGG 0.547639 5.4:-78280470 MS.gene074488:intron
AAACGGAGATGGCGGAAATG+AGG 0.548034 5.4:+78281714 None:intergenic
GAGAGCTCTGAGGGGTGCTA+GGG 0.550613 5.4:+78279147 None:intergenic
TGGCGGAAATGAGGAGGCGA+TGG 0.552680 5.4:+78281723 None:intergenic
GCGATTAACAAGTATCGATC+CGG 0.554337 5.4:+78281157 None:intergenic
AGAAAGCAGGTTCAGCTCAA+AGG 0.555946 5.4:-78278481 MS.gene074488:CDS
AACAAACAACAATGGGAACA+TGG 0.556243 5.4:-78279002 MS.gene074488:CDS
CGAGTTCGAACTTTCCAGGT+AGG 0.556717 5.4:-78278727 MS.gene074488:CDS
AACTCTTATGTCATTCCGAA+GGG 0.557571 5.4:+78280200 None:intergenic
AAATGGGGCCAATTCCTCCT+TGG 0.558531 5.4:+78279090 None:intergenic
TTTCCGTTAGGGCGGATGGT+AGG 0.563392 5.4:+78278808 None:intergenic
AATCCGTAAATTTGAGAGCA+TGG 0.564183 5.4:+78279792 None:intergenic
TGGGAAATTTGTTGGTTGAG+CGG 0.564822 5.4:-78279757 MS.gene074488:CDS
TTGTTAATCGCAACCAGTGG+AGG 0.567512 5.4:-78281145 MS.gene074488:CDS
AATTGAAACGCATTTGTATG+TGG 0.568739 5.4:-78279200 MS.gene074488:CDS
TTGGTCAGTACCCATTAGAA+GGG 0.570383 5.4:+78278438 None:intergenic
AAACCTACCATCCGCCCTAA+CGG 0.572836 5.4:-78278811 MS.gene074488:CDS
TATTCTTGTTCATGAATAAG+GGG 0.577205 5.4:+78278776 None:intergenic
GGCGAAGGAAACTGGCAAGA+AGG 0.588540 5.4:+78281756 None:intergenic
TCTTGCATATGTCAAAACTC+AGG 0.590986 5.4:-78278643 MS.gene074488:CDS
GATGGATGAGACGACGGCGA+AGG 0.593369 5.4:+78281741 None:intergenic
GAGAAGGCAAACGGAGATGG+CGG 0.595462 5.4:+78281706 None:intergenic
CCACTTACCCCAGAGGAAGT+TGG 0.600004 5.4:-78279250 MS.gene074488:CDS
GCATGATGAAAAGAAAGAGA+AGG 0.602057 5.4:+78281690 None:intergenic
GCTTAAATCAACAGAGAACA+GGG 0.602960 5.4:-78281123 MS.gene074488:CDS
AAGAGCATTTACAATAAAAG+GGG 0.610292 5.4:+78281649 None:intergenic
AATGAGTTGAGGTCAGGCAG+TGG 0.610497 5.4:-78278688 MS.gene074488:CDS
TCTCCTTTGAATGGAAGTTG+GGG 0.613782 5.4:+78279122 None:intergenic
CTTGCATATGTCAAAACTCA+GGG 0.615011 5.4:-78278642 MS.gene074488:CDS
CGGAGATGGCGGAAATGAGG+AGG 0.616366 5.4:+78281717 None:intergenic
GACAAGTCAAGAAGACTCAT+CGG 0.616470 5.4:-78281782 MS.gene074488:CDS
GCATTTACAATAAAAGGGGA+AGG 0.618091 5.4:+78281653 None:intergenic
AAAGGAGACCTTAGCTACCA+AGG 0.620774 5.4:-78279107 MS.gene074488:CDS
AACTTGGTGAAATTAGGAAG+CGG 0.626657 5.4:-78279415 MS.gene074488:CDS
TGGAGAAATATATGGAGGCG+AGG 0.630114 5.4:-78279173 MS.gene074488:CDS
ATGTCAAAACTCAGGGTCGG+AGG 0.632035 5.4:-78278635 MS.gene074488:CDS
TACTTGTTAATCGCAACCAG+TGG 0.633957 5.4:-78281148 MS.gene074488:CDS
TTTACTTGCAAAGTTGACAC+CGG 0.634644 5.4:+78281303 None:intergenic
GACAGAAAACCAGCCTGCTG+AGG 0.636575 5.4:-78278548 MS.gene074488:CDS
TGGGAGAGCCTAATGAGTTG+AGG 0.637626 5.4:-78278699 MS.gene074488:CDS
TGGTCAGTACCCATTAGAAG+GGG 0.642645 5.4:+78278439 None:intergenic
TTGGTGAAATTAGGAAGCGG+TGG 0.644603 5.4:-78279412 MS.gene074488:CDS
AGAAAGTAAGAAGACATGGA+AGG 0.646390 5.4:-78279276 MS.gene074488:CDS
CATATGTCAAAACTCAGGGT+CGG 0.649933 5.4:-78278638 MS.gene074488:CDS
CTGCTGAGGAAGTACGAGAG+TGG 0.653675 5.4:-78278534 MS.gene074488:CDS
TCTACAAAAGCTGAGGTGCA+GGG 0.657185 5.4:-78279822 MS.gene074488:CDS
TGAACAAGAATAACATGACA+TGG 0.657343 5.4:-78278765 MS.gene074488:CDS
AGTTGGGGAAGAGAGCTCTG+AGG 0.685910 5.4:+78279137 None:intergenic
GGAGGCGATGGATGAGACGA+CGG 0.693598 5.4:+78281735 None:intergenic
GCTTCTGGAGAAATATATGG+AGG 0.698383 5.4:-78279178 MS.gene074488:CDS
GCGACCCTCGTAGCATTTGG+GGG 0.700748 5.4:+78280107 None:intergenic
TGAAGAGAAAGACACAAATG+GGG 0.704290 5.4:+78279075 None:intergenic
GGTCAGTACCCATTAGAAGG+GGG 0.707285 5.4:+78278440 None:intergenic
ATGATGATCTACAAAAGCTG+AGG 0.712332 5.4:-78279829 MS.gene074488:CDS
CATAAGACCAACTTCCTCTG+GGG 0.724014 5.4:+78279243 None:intergenic
CTTAAATCAACAGAGAACAG+GGG 0.728232 5.4:-78281122 MS.gene074488:intron
AAGGTGTCCACTTACCCCAG+AGG 0.746305 5.4:-78279257 MS.gene074488:CDS
TTGGGGAAGAGAGCTCTGAG+GGG 0.774845 5.4:+78279139 None:intergenic
GGAGACCTTAGCTACCAAGG+AGG 0.785211 5.4:-78279104 MS.gene074488:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAGTTATAATTATCAAA+TGG + chr5.4:78280556-78280575 None:intergenic 10.0%
!! TATATTATAAATGAAATAGA+AGG + chr5.4:78278634-78278653 None:intergenic 10.0%
!!! AAAATGTAAATTATTGTTTT+AGG + chr5.4:78280632-78280651 None:intergenic 10.0%
!! AAAAATTATAGATTGTTTGT+GGG - chr5.4:78279590-78279609 MS.gene074488:intron 15.0%
!! AATCAAAAGCAATTAAATAT+AGG + chr5.4:78279008-78279027 None:intergenic 15.0%
!! CAAAATGCATAAAAAAAAAA+AGG + chr5.4:78278794-78278813 None:intergenic 15.0%
!! CAAATATTACATACAAATTA+AGG + chr5.4:78279959-78279978 None:intergenic 15.0%
!! TTAAATGTTATACAATCATA+TGG + chr5.4:78280330-78280349 None:intergenic 15.0%
!!! ACTATTTTATATAGATATAG+TGG - chr5.4:78279353-78279372 MS.gene074488:intron 15.0%
!!! ATCACATATAATATTTTCTT+TGG - chr5.4:78279907-78279926 MS.gene074488:intron 15.0%
!!! ATGAAAATAATTTTGATGAA+AGG + chr5.4:78280216-78280235 None:intergenic 15.0%
!! AAAAAATACAGATTGTTTGT+GGG - chr5.4:78279744-78279763 MS.gene074488:CDS 20.0%
!! AAAGAAAATGAAATTGAAGT+TGG + chr5.4:78280679-78280698 None:intergenic 20.0%
!! ATAAAAAAAAAAAGGTGAGA+AGG + chr5.4:78278786-78278805 None:intergenic 20.0%
!! GAAAAATTATAGATTGTTTG+TGG - chr5.4:78279589-78279608 MS.gene074488:intron 20.0%
!! TTATTCTTGTTCATGAATAA+GGG + chr5.4:78281466-78281485 None:intergenic 20.0%
!!! TTGTTTTTTCTGTTTCATAT+AGG - chr5.4:78280736-78280755 MS.gene074488:intron 20.0%
!!! TTTTGTTTATTGGAATTTGA+AGG - chr5.4:78278840-78278859 MS.gene074488:intron 20.0%
!!! TTTTTGTAACAAACAACAAT+GGG - chr5.4:78281229-78281248 MS.gene074488:intron 20.0%
!!! TTTTTTATGCATTTTGTGTT+AGG - chr5.4:78278798-78278817 MS.gene074488:CDS 20.0%
! AACATTTAATTGCATTACAC+AGG - chr5.4:78280341-78280360 MS.gene074488:intron 25.0%
! AAGGAGGATAACTTATTAAT+CGG - chr5.4:78279235-78279254 MS.gene074488:CDS 25.0%
! AATCTCTACCCAATAATTAT+AGG - chr5.4:78279409-78279428 MS.gene074488:CDS 25.0%
! ACAATTATCACATTGAACTT+CGG + chr5.4:78280703-78280722 None:intergenic 25.0%
! ACAATTCATGAGATGAAAAA+AGG + chr5.4:78278981-78279000 None:intergenic 25.0%
! CTTAATTTATTCCAAGTAGT+TGG - chr5.4:78281329-78281348 MS.gene074488:CDS 25.0%
! GAAAAAATACAGATTGTTTG+TGG - chr5.4:78279743-78279762 MS.gene074488:CDS 25.0%
! GAATGATATATTACGTTATG+TGG - chr5.4:78279544-78279563 MS.gene074488:intron 25.0%
! GTCTGAATTAGATTATGATT+TGG - chr5.4:78281726-78281745 MS.gene074488:CDS 25.0%
! GTTATTCTTGTTCATGAATA+AGG + chr5.4:78281467-78281486 None:intergenic 25.0%
! TATAACTAAATATTTGTCCC+TGG - chr5.4:78280565-78280584 MS.gene074488:intron 25.0%
! TATTCTTGTTCATGAATAAG+GGG + chr5.4:78281465-78281484 None:intergenic 25.0%
! TGTAACTTTGATTGTATTTC+AGG - chr5.4:78278686-78278705 MS.gene074488:CDS 25.0%
! TGTATTTCAGGAAAATGATT+TGG - chr5.4:78278698-78278717 MS.gene074488:CDS 25.0%
!! GTTTTTGTAACAAACAACAA+TGG - chr5.4:78281228-78281247 MS.gene074488:intron 25.0%
!! TGGTCATTTACAAAAAGTAT+AGG - chr5.4:78279382-78279401 MS.gene074488:intron 25.0%
!!! AAGAGCATTTACAATAAAAG+GGG + chr5.4:78278592-78278611 None:intergenic 25.0%
!!! ATACTTTTTGTAAATGACCA+CGG + chr5.4:78279382-78279401 None:intergenic 25.0%
!!! GAAGAGCATTTACAATAAAA+GGG + chr5.4:78278593-78278612 None:intergenic 25.0%
!!! TCCTAATTGTGTTTTTTTAG+CGG + chr5.4:78279482-78279501 None:intergenic 25.0%
!!! TCGATCTTCTTTTTGTTTAT+TGG - chr5.4:78278830-78278849 MS.gene074488:CDS 25.0%
!!! TGAAGAGCATTTACAATAAA+AGG + chr5.4:78278594-78278613 None:intergenic 25.0%
AAAGATGTATGATCCAATTC+TGG + chr5.4:78279826-78279845 None:intergenic 30.0%
AAATTTACGGATTCGATTCA+GGG - chr5.4:78280456-78280475 MS.gene074488:CDS 30.0%
AACAGAAAAAACAAGCAGTT+TGG + chr5.4:78280730-78280749 None:intergenic 30.0%
AAGAGTAGCATTCAAAAGAT+AGG + chr5.4:78279797-78279816 None:intergenic 30.0%
AAGGGATGTTATAAACCAAT+CGG + chr5.4:78280023-78280042 None:intergenic 30.0%
AATTGAAACGCATTTGTATG+TGG - chr5.4:78281038-78281057 MS.gene074488:intron 30.0%
AATTGGATCATACATCTTTC+TGG - chr5.4:78279827-78279846 MS.gene074488:CDS 30.0%
ACAAATTTCTCTCCCATATT+TGG + chr5.4:78280076-78280095 None:intergenic 30.0%
ATTATAGGTCTGCAACAAAT+TGG - chr5.4:78279424-78279443 MS.gene074488:CDS 30.0%
ATTTCACTTCTATCATATCC+AGG - chr5.4:78279873-78279892 MS.gene074488:CDS 30.0%
CTAGATCAAATTTGATGAAG+TGG - chr5.4:78279701-78279720 MS.gene074488:intron 30.0%
GAAAAGGAAAGAAAAGAACT+TGG - chr5.4:78280807-78280826 MS.gene074488:intron 30.0%
GATTAATAAGTTATCCTCCT+TGG + chr5.4:78279236-78279255 None:intergenic 30.0%
GGTGGAAAAATTTACATGTA+AGG - chr5.4:78280844-78280863 MS.gene074488:intron 30.0%
GTTGCAGACCTATAATTATT+GGG + chr5.4:78279420-78279439 None:intergenic 30.0%
TCCGCTAAAAAAACACAATT+AGG - chr5.4:78279478-78279497 MS.gene074488:CDS 30.0%
TCGGCTAAAATAACTCAATT+AGG - chr5.4:78279673-78279692 MS.gene074488:intron 30.0%
TCTAATTAAAGGTCCCTAAT+CGG - chr5.4:78280188-78280207 MS.gene074488:CDS 30.0%
TGAACAAGAATAACATGACA+TGG - chr5.4:78281473-78281492 MS.gene074488:intron 30.0%
TGATAATTAATGTGACTGAC+AGG - chr5.4:78280927-78280946 MS.gene074488:intron 30.0%
TGCTACAATAACTAGTATGT+TGG + chr5.4:78280294-78280313 None:intergenic 30.0%
TGTTGCAGACCTATAATTAT+TGG + chr5.4:78279421-78279440 None:intergenic 30.0%
TGTTTCATATAGGTTTGAAC+CGG - chr5.4:78280746-78280765 MS.gene074488:intron 30.0%
TTTGGACAATTATAGAAAGC+AGG - chr5.4:78281744-78281763 MS.gene074488:CDS 30.0%
! AGAAGGTAAGCTAGTTTATT+AGG - chr5.4:78281276-78281295 MS.gene074488:intron 30.0%
! ATTGGTTTATAACATCCCTT+CGG - chr5.4:78280023-78280042 MS.gene074488:intron 30.0%
! GAAAAGAACTTGGTGAAATT+AGG - chr5.4:78280817-78280836 MS.gene074488:intron 30.0%
! GAAGGTAAGCTAGTTTATTA+GGG - chr5.4:78281277-78281296 MS.gene074488:intron 30.0%
!! CAAATTTTGTATGTGCCATT+CGG - chr5.4:78279654-78279673 MS.gene074488:intron 30.0%
!! CATATTTCTAACTTTTGCAG+AGG - chr5.4:78279041-78279060 MS.gene074488:CDS 30.0%
!! TACTCTTGTTATTCCAGAAT+TGG - chr5.4:78279810-78279829 MS.gene074488:CDS 30.0%
!!! ATTGAGTTATTTTAGCCGAA+TGG + chr5.4:78279672-78279691 None:intergenic 30.0%
!!! ATTGTGTTTTTTTAGCGGAA+TGG + chr5.4:78279477-78279496 None:intergenic 30.0%
!!! TTGTGTTTTTTTAGCGGAAT+GGG + chr5.4:78279476-78279495 None:intergenic 30.0%
AACAAACAACAATGGGAACA+TGG - chr5.4:78281236-78281255 MS.gene074488:intron 35.0%
AACATGGCTAAAATTCTAGC+TGG - chr5.4:78281252-78281271 MS.gene074488:intron 35.0%
AACTCTTATGTCATTCCGAA+GGG + chr5.4:78280041-78280060 None:intergenic 35.0%
AAGGCAATAAGGTAGAAAAC+AGG - chr5.4:78281668-78281687 MS.gene074488:CDS 35.0%
AATCCGTAAATTTGAGAGCA+TGG + chr5.4:78280449-78280468 None:intergenic 35.0%
AGAAAGTAAGAAGACATGGA+AGG - chr5.4:78280962-78280981 MS.gene074488:intron 35.0%
ATGATGATCTACAAAAGCTG+AGG - chr5.4:78280409-78280428 MS.gene074488:CDS 35.0%
ATGTGATTTAAGAGACATCC+TGG + chr5.4:78279894-78279913 None:intergenic 35.0%
ATTCAGACTTTCTCTGTTGT+AGG - chr5.4:78281389-78281408 MS.gene074488:intron 35.0%
ATTGCATTACACAGGTTGTT+CGG - chr5.4:78280349-78280368 MS.gene074488:intron 35.0%
CAAATTGATACGAAAGCTCT+AGG - chr5.4:78280653-78280672 MS.gene074488:intron 35.0%
CAAATTTACGGATTCGATTC+AGG - chr5.4:78280455-78280474 MS.gene074488:CDS 35.0%
CATGAGATGAAAAAAGGACT+GGG + chr5.4:78278975-78278994 None:intergenic 35.0%
CCAAATAGAAACCAACTACT+TGG + chr5.4:78281343-78281362 None:intergenic 35.0%
CTACCATGCTCTCAAATTTA+CGG - chr5.4:78280443-78280462 MS.gene074488:CDS 35.0%
CTTAAATCAACAGAGAACAG+GGG - chr5.4:78279116-78279135 MS.gene074488:CDS 35.0%
CTTGCATATGTCAAAACTCA+GGG - chr5.4:78281596-78281615 MS.gene074488:intron 35.0%
GAAAAGAAAGAGAAGGCAAA+CGG + chr5.4:78278544-78278563 None:intergenic 35.0%
GCATGATGAAAAGAAAGAGA+AGG + chr5.4:78278551-78278570 None:intergenic 35.0%
GCATTTACAATAAAAGGGGA+AGG + chr5.4:78278588-78278607 None:intergenic 35.0%
GCGTGTAAGTGTCTAATTAA+AGG - chr5.4:78280177-78280196 MS.gene074488:CDS 35.0%
GCTTAAATCAACAGAGAACA+GGG - chr5.4:78279115-78279134 MS.gene074488:CDS 35.0%
GGATACCTAACTTACAATGT+TGG + chr5.4:78280273-78280292 None:intergenic 35.0%
GTAAAGCAGCTACCAAATAT+GGG - chr5.4:78280061-78280080 MS.gene074488:intron 35.0%
GTCTGAATATCAGTATGTGT+AGG + chr5.4:78281377-78281396 None:intergenic 35.0%
GTGAAGAGAAAGACACAAAT+GGG + chr5.4:78281167-78281186 None:intergenic 35.0%
TAGAAGGTAGTTGAAGTAGA+AGG + chr5.4:78278618-78278637 None:intergenic 35.0%
TCATGAGATGAAAAAAGGAC+TGG + chr5.4:78278976-78278995 None:intergenic 35.0%
TCTTGCATATGTCAAAACTC+AGG - chr5.4:78281595-78281614 MS.gene074488:intron 35.0%
TGAAGAGAAAGACACAAATG+GGG + chr5.4:78281166-78281185 None:intergenic 35.0%
TGAAGTGGATGCAATATTTC+TGG - chr5.4:78279716-78279735 MS.gene074488:CDS 35.0%
TGAGAAGGTTGTTTGATTAC+CGG + chr5.4:78278771-78278790 None:intergenic 35.0%
TGGTTCCAACATTGTAAGTT+AGG - chr5.4:78280265-78280284 MS.gene074488:intron 35.0%
TGTAAAGCAGCTACCAAATA+TGG - chr5.4:78280060-78280079 MS.gene074488:intron 35.0%
TGTGGATGATGCAATATTTC+TGG - chr5.4:78279562-78279581 MS.gene074488:intron 35.0%
TTCTTTCTCTTCACTGATGA+TGG + chr5.4:78278420-78278439 None:intergenic 35.0%
TTGATGAAAGGTTCCGATTA+GGG + chr5.4:78280204-78280223 None:intergenic 35.0%
TTGATTACCGGAATGTGAAA+TGG + chr5.4:78278759-78278778 None:intergenic 35.0%
TTTACTTGCAAAGTTGACAC+CGG + chr5.4:78278938-78278957 None:intergenic 35.0%
TTTGATGAAAGGTTCCGATT+AGG + chr5.4:78280205-78280224 None:intergenic 35.0%
TTTGTGGGTGCAAGAATTAT+AGG - chr5.4:78279605-78279624 MS.gene074488:intron 35.0%
! AACTTGGTGAAATTAGGAAG+CGG - chr5.4:78280823-78280842 MS.gene074488:intron 35.0%
! CCAAGTAGTTGGTTTCTATT+TGG - chr5.4:78281340-78281359 MS.gene074488:CDS 35.0%
! CTAAAATTCTAGCTGGAAGA+AGG - chr5.4:78281259-78281278 MS.gene074488:intron 35.0%
! CTCAATTATTTGTTGCCGAT+TGG - chr5.4:78280005-78280024 MS.gene074488:intron 35.0%
! TTTTGACATATGCAAGACGT+TGG + chr5.4:78281592-78281611 None:intergenic 35.0%
!! ATGCGTTTGATTTTGAGGAT+TGG + chr5.4:78281647-78281666 None:intergenic 35.0%
!!! GGTTCAACTTTTTTCCGTTA+GGG + chr5.4:78281444-78281463 None:intergenic 35.0%
AATGGCATATCAAAGGAACC+AGG + chr5.4:78280586-78280605 None:intergenic 40.0%
AGAATACTTGCACTTCAGCT+TGG + chr5.4:78281822-78281841 None:intergenic 40.0%
AGGCAAATGAAGCTACTTAC+AGG - chr5.4:78278860-78278879 MS.gene074488:intron 40.0%
ATGGCATATCAAAGGAACCA+GGG + chr5.4:78280585-78280604 None:intergenic 40.0%
CAACTCTTATGTCATTCCGA+AGG + chr5.4:78280042-78280061 None:intergenic 40.0%
CATATGTCAAAACTCAGGGT+CGG - chr5.4:78281600-78281619 MS.gene074488:intron 40.0%
CCATGTCTTCTTACTTTCTC+AGG + chr5.4:78280961-78280980 None:intergenic 40.0%
CCTCAAAATCAAACGCATGA+AGG - chr5.4:78281649-78281668 MS.gene074488:intron 40.0%
CCTGAGAAAGTAAGAAGACA+TGG - chr5.4:78280958-78280977 MS.gene074488:intron 40.0%
CGTGAAGAGAAAGACACAAA+TGG + chr5.4:78281168-78281187 None:intergenic 40.0%
CTACAGACTTTCTAATCTGC+TGG - chr5.4:78280386-78280405 MS.gene074488:intron 40.0%
CTACTTACAGGCATGATCTA+TGG - chr5.4:78278872-78278891 MS.gene074488:intron 40.0%
GACAAGTCAAGAAGACTCAT+CGG - chr5.4:78278456-78278475 MS.gene074488:CDS 40.0%
GATCGAATTTGACGAATGAG+TGG - chr5.4:78279509-78279528 MS.gene074488:intron 40.0%
GCAGATAATTGATGCTGTTG+TGG - chr5.4:78279768-78279787 MS.gene074488:CDS 40.0%
GCTGAAGAATGGCATATCAA+AGG + chr5.4:78280593-78280612 None:intergenic 40.0%
GGCTAATACCAAAACTCATC+CGG - chr5.4:78279062-78279081 MS.gene074488:CDS 40.0%
GGCTTAAATCAACAGAGAAC+AGG - chr5.4:78279114-78279133 MS.gene074488:CDS 40.0%
GTCTCCTTTGAATGGAAGTT+GGG + chr5.4:78281120-78281139 None:intergenic 40.0%
GTTTCATCGCGCTACATTTA+AGG - chr5.4:78280523-78280542 MS.gene074488:intron 40.0%
TAGCTAAGGTCTCCTTTGAA+TGG + chr5.4:78281128-78281147 None:intergenic 40.0%
TCAAACGCATGAAGGCAATA+AGG - chr5.4:78281657-78281676 MS.gene074488:intron 40.0%
TCAGGGAATGGGAAATTTGT+TGG - chr5.4:78280473-78280492 MS.gene074488:intron 40.0%
TCTCCTTTGAATGGAAGTTG+GGG + chr5.4:78281119-78281138 None:intergenic 40.0%
TGAATGTATAGAGCAGTCCA+AGG - chr5.4:78279216-78279235 MS.gene074488:CDS 40.0%
TTGGTCAGTACCCATTAGAA+GGG + chr5.4:78281803-78281822 None:intergenic 40.0%
! CATCTTTCTGGAAAGACAAC+AGG - chr5.4:78279839-78279858 MS.gene074488:CDS 40.0%
! GCATTCTCTGGTTGCTATTA+TGG - chr5.4:78280777-78280796 MS.gene074488:intron 40.0%
! GCTTCTGGAGAAATATATGG+AGG - chr5.4:78281060-78281079 MS.gene074488:intron 40.0%
! GTGGCTTCTGGAGAAATATA+TGG - chr5.4:78281057-78281076 MS.gene074488:intron 40.0%
! TACTTGTTAATCGCAACCAG+TGG - chr5.4:78279090-78279109 MS.gene074488:CDS 40.0%
! TCTCTGGTTGCTATTATGGT+GGG - chr5.4:78280781-78280800 MS.gene074488:intron 40.0%
! TTCTCTGGTTGCTATTATGG+TGG - chr5.4:78280780-78280799 MS.gene074488:intron 40.0%
!! ATGCTTAGAGCACTAGGTTT+TGG - chr5.4:78281015-78281034 MS.gene074488:intron 40.0%
!! CCTTCATGCGTTTGATTTTG+AGG + chr5.4:78281652-78281671 None:intergenic 40.0%
!! GCGATTAACAAGTATCGATC+CGG + chr5.4:78279084-78279103 None:intergenic 40.0%
!! TGGGAAATTTGTTGGTTGAG+CGG - chr5.4:78280481-78280500 MS.gene074488:intron 40.0%
!!! GGGTTCAACTTTTTTCCGTT+AGG + chr5.4:78281445-78281464 None:intergenic 40.0%
!!! TCAACTTTTTTCCGTTAGGG+CGG + chr5.4:78281441-78281460 None:intergenic 40.0%
AAAGAGAAGGCAAACGGAGA+TGG + chr5.4:78278538-78278557 None:intergenic 45.0%
AAAGGAGACCTTAGCTACCA+AGG - chr5.4:78281131-78281150 MS.gene074488:CDS 45.0%
AACTTTCCAGGTAGGCTTCA+TGG - chr5.4:78281519-78281538 MS.gene074488:CDS 45.0%
ACGCATTTGTATGTGGCTTC+TGG - chr5.4:78281045-78281064 MS.gene074488:intron 45.0%
ACGGATTCGATTCAGGGAAT+GGG - chr5.4:78280462-78280481 MS.gene074488:CDS 45.0%
ACTTTCCAGGTAGGCTTCAT+GGG - chr5.4:78281520-78281539 MS.gene074488:CDS 45.0%
AGAAAGCAGGTTCAGCTCAA+AGG - chr5.4:78281757-78281776 MS.gene074488:CDS 45.0%
AGCATAAGACCAACTTCCTC+TGG + chr5.4:78281000-78281019 None:intergenic 45.0%
ATCTACAAAAGCTGAGGTGC+AGG - chr5.4:78280415-78280434 MS.gene074488:CDS 45.0%
ATCTCGAGTTCGAACTTTCC+AGG - chr5.4:78281507-78281526 MS.gene074488:CDS 45.0%
ATGTATAGAGCAGTCCAAGG+AGG - chr5.4:78279219-78279238 MS.gene074488:CDS 45.0%
CAGACTTTCTCTGTTGTAGG+AGG - chr5.4:78281392-78281411 MS.gene074488:intron 45.0%
CATAAGACCAACTTCCTCTG+GGG + chr5.4:78280998-78281017 None:intergenic 45.0%
CATATACAGTGCGTGTTCCA+AGG - chr5.4:78280101-78280120 MS.gene074488:CDS 45.0%
CCAGAGAATGCAAGCATATC+CGG + chr5.4:78280768-78280787 None:intergenic 45.0%
CTTAGCTACCAAGGAGGAAT+TGG - chr5.4:78281140-78281159 MS.gene074488:CDS 45.0%
CTTCCCCAACTTCCATTCAA+AGG - chr5.4:78281113-78281132 MS.gene074488:intron 45.0%
CTTGGAACACGCACTGTATA+TGG + chr5.4:78280103-78280122 None:intergenic 45.0%
CTTGGTCAGTACCCATTAGA+AGG + chr5.4:78281804-78281823 None:intergenic 45.0%
GAAAAAAGGACTGGGAGTCT+AGG + chr5.4:78278967-78278986 None:intergenic 45.0%
GCAATCGCCATTTCACATTC+CGG - chr5.4:78278749-78278768 MS.gene074488:CDS 45.0%
GCATAAGACCAACTTCCTCT+GGG + chr5.4:78280999-78281018 None:intergenic 45.0%
GCTGATCACATGCTGAAGAA+TGG + chr5.4:78280604-78280623 None:intergenic 45.0%
GGTCTCCTTTGAATGGAAGT+TGG + chr5.4:78281121-78281140 None:intergenic 45.0%
TACGGATTCGATTCAGGGAA+TGG - chr5.4:78280461-78280480 MS.gene074488:CDS 45.0%
TATAGATATAGTGGCGACCG+TGG - chr5.4:78279362-78279381 MS.gene074488:intron 45.0%
TCTACAAAAGCTGAGGTGCA+GGG - chr5.4:78280416-78280435 MS.gene074488:CDS 45.0%
TGGAGAAATATATGGAGGCG+AGG - chr5.4:78281065-78281084 MS.gene074488:intron 45.0%
TGGTCAGTACCCATTAGAAG+GGG + chr5.4:78281802-78281821 None:intergenic 45.0%
TGTGTCTTTCTCTTCACGCA+TGG - chr5.4:78281170-78281189 MS.gene074488:CDS 45.0%
TTCTCTGCCTGTAGTAATGC+CGG - chr5.4:78278916-78278935 MS.gene074488:intron 45.0%
TTGGTGAAATTAGGAAGCGG+TGG - chr5.4:78280826-78280845 MS.gene074488:intron 45.0%
TTGTTAATCGCAACCAGTGG+AGG - chr5.4:78279093-78279112 MS.gene074488:CDS 45.0%
! CAATTCCTCCTTGGTAGCTA+AGG + chr5.4:78281142-78281161 None:intergenic 45.0%
! CTATTATGGTGGGGGAGAAA+AGG - chr5.4:78280791-78280810 MS.gene074488:intron 45.0%
! CTCTGGTTGCTATTATGGTG+GGG - chr5.4:78280782-78280801 MS.gene074488:intron 45.0%
! GGTCTTATGCTTAGAGCACT+AGG - chr5.4:78281009-78281028 MS.gene074488:intron 45.0%
! TCTGGTTGCTATTATGGTGG+GGG - chr5.4:78280783-78280802 MS.gene074488:intron 45.0%
!! CTCTGTTGTAGGAGGTACTT+TGG - chr5.4:78281400-78281419 MS.gene074488:intron 45.0%
!! CTGTTGATTTAAGCCTCCAC+TGG + chr5.4:78279109-78279128 None:intergenic 45.0%
!! GTATCGATCCGGATGAGTTT+TGG + chr5.4:78279073-78279092 None:intergenic 45.0%
!! AAAATTTATTAATAAAATTG+AGG + chr5.4:78280244-78280263 None:intergenic 5.0%
!! AAATTTATTAATAAAATTGA+GGG + chr5.4:78280243-78280262 None:intergenic 5.0%
!!! AATTTTATTAATAAATTTTG+TGG - chr5.4:78280245-78280264 MS.gene074488:intron 5.0%
AAACCTACCATCCGCCCTAA+CGG - chr5.4:78281427-78281446 MS.gene074488:intron 50.0%
AAATGGGGCCAATTCCTCCT+TGG + chr5.4:78281151-78281170 None:intergenic 50.0%
AGGGTCGGAGGTAAAAACTG+AGG - chr5.4:78281615-78281634 MS.gene074488:intron 50.0%
ATGTCAAAACTCAGGGTCGG+AGG - chr5.4:78281603-78281622 MS.gene074488:intron 50.0%
CCGGATATGCTTGCATTCTC+TGG - chr5.4:78280765-78280784 MS.gene074488:intron 50.0%
CGAGTTCGAACTTTCCAGGT+AGG - chr5.4:78281511-78281530 MS.gene074488:CDS 50.0%
CTGCCTGACCTCAACTCATT+AGG + chr5.4:78281550-78281569 None:intergenic 50.0%
GGGTCGGAGGTAAAAACTGA+GGG - chr5.4:78281616-78281635 MS.gene074488:intron 50.0%
GGTCAGTACCCATTAGAAGG+GGG + chr5.4:78281801-78281820 None:intergenic 50.0%
GTTGACACCGGCATTACTAC+AGG + chr5.4:78278926-78278945 None:intergenic 50.0%
TGGGAGAGCCTAATGAGTTG+AGG - chr5.4:78281539-78281558 MS.gene074488:intron 50.0%
! AATGAGTTGAGGTCAGGCAG+TGG - chr5.4:78281550-78281569 MS.gene074488:intron 50.0%
! CATTTGGGGGTGCATTTCCT+TGG + chr5.4:78280121-78280140 None:intergenic 50.0%
! GAGCCTAATGAGTTGAGGTC+AGG - chr5.4:78281544-78281563 MS.gene074488:intron 50.0%
!! AAACGGAGATGGCGGAAATG+AGG + chr5.4:78278527-78278546 None:intergenic 50.0%
!!! CTTTTTTCCGTTAGGGCGGA+TGG + chr5.4:78281437-78281456 None:intergenic 50.0%
AACGCGACCCTCGTAGCATT+TGG + chr5.4:78280137-78280156 None:intergenic 55.0%
ACACGCTTCTTGACCAGCAC+TGG + chr5.4:78280163-78280182 None:intergenic 55.0%
ACGCGACCCTCGTAGCATTT+GGG + chr5.4:78280136-78280155 None:intergenic 55.0%
AGTTGGGGAAGAGAGCTCTG+AGG + chr5.4:78281104-78281123 None:intergenic 55.0%
ATGCACCCCCAAATGCTACG+AGG - chr5.4:78280126-78280145 MS.gene074488:CDS 55.0%
CACTCTCGTACTTCCTCAGC+AGG + chr5.4:78281706-78281725 None:intergenic 55.0%
CCACTTACCCCAGAGGAAGT+TGG - chr5.4:78280988-78281007 MS.gene074488:intron 55.0%
GACAGAAAACCAGCCTGCTG+AGG - chr5.4:78281690-78281709 MS.gene074488:CDS 55.0%
GAGAAGGCAAACGGAGATGG+CGG + chr5.4:78278535-78278554 None:intergenic 55.0%
GAGTCGCTCCCCCTTCTAAT+GGG - chr5.4:78281790-78281809 MS.gene074488:CDS 55.0%
GGAGACCTTAGCTACCAAGG+AGG - chr5.4:78281134-78281153 MS.gene074488:CDS 55.0%
GGCGAAGGAAACTGGCAAGA+AGG + chr5.4:78278485-78278504 None:intergenic 55.0%
TGAGTCGCTCCCCCTTCTAA+TGG - chr5.4:78281789-78281808 MS.gene074488:CDS 55.0%
TGCACCCCCAAATGCTACGA+GGG - chr5.4:78280127-78280146 MS.gene074488:CDS 55.0%
TTTCCGTTAGGGCGGATGGT+AGG + chr5.4:78281433-78281452 None:intergenic 55.0%
! AAGGTGTCCACTTACCCCAG+AGG - chr5.4:78280981-78281000 MS.gene074488:intron 55.0%
! CTGCTGAGGAAGTACGAGAG+TGG - chr5.4:78281704-78281723 MS.gene074488:CDS 55.0%
! GTTGGGGAAGAGAGCTCTGA+GGG + chr5.4:78281103-78281122 None:intergenic 55.0%
!! CCAACTTCCTCTGGGGTAAG+TGG + chr5.4:78280991-78281010 None:intergenic 55.0%
!! TTGGGGAAGAGAGCTCTGAG+GGG + chr5.4:78281102-78281121 None:intergenic 55.0%
CGCGACCCTCGTAGCATTTG+GGG + chr5.4:78280135-78280154 None:intergenic 60.0%
CTCGTACTTCCTCAGCAGGC+TGG + chr5.4:78281702-78281721 None:intergenic 60.0%
GAGACGACGGCGAAGGAAAC+TGG + chr5.4:78278493-78278512 None:intergenic 60.0%
GATGGATGAGACGACGGCGA+AGG + chr5.4:78278500-78278519 None:intergenic 60.0%
GCGACCCTCGTAGCATTTGG+GGG + chr5.4:78280134-78280153 None:intergenic 60.0%
GCTCTCCCATGAAGCCTACC+TGG + chr5.4:78281528-78281547 None:intergenic 60.0%
GGAGGCGATGGATGAGACGA+CGG + chr5.4:78278506-78278525 None:intergenic 60.0%
TGGCGGAAATGAGGAGGCGA+TGG + chr5.4:78278518-78278537 None:intergenic 60.0%
! GGGTCGCGTTTTACCAGTGC+TGG - chr5.4:78280147-78280166 MS.gene074488:CDS 60.0%
!! AGAGAGCTCTGAGGGGTGCT+AGG + chr5.4:78281095-78281114 None:intergenic 60.0%
!! CGGAGATGGCGGAAATGAGG+AGG + chr5.4:78278524-78278543 None:intergenic 60.0%
!! GAGAGCTCTGAGGGGTGCTA+GGG + chr5.4:78281094-78281113 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 78278408 78281852 78278408 ID=MS.gene074488
chr5.4 mRNA 78278408 78281852 78278408 ID=MS.gene074488.t1;Parent=MS.gene074488
chr5.4 exon 78281661 78281852 78281661 ID=MS.gene074488.t1.exon1;Parent=MS.gene074488.t1
chr5.4 CDS 78281661 78281852 78281661 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78281490 78281552 78281490 ID=MS.gene074488.t1.exon2;Parent=MS.gene074488.t1
chr5.4 CDS 78281490 78281552 78281490 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78281308 78281398 78281308 ID=MS.gene074488.t1.exon3;Parent=MS.gene074488.t1
chr5.4 CDS 78281308 78281398 78281308 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78281123 78281199 78281123 ID=MS.gene074488.t1.exon4;Parent=MS.gene074488.t1
chr5.4 CDS 78281123 78281199 78281123 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78280400 78280488 78280400 ID=MS.gene074488.t1.exon5;Parent=MS.gene074488.t1
chr5.4 CDS 78280400 78280488 78280400 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78280080 78280263 78280080 ID=MS.gene074488.t1.exon6;Parent=MS.gene074488.t1
chr5.4 CDS 78280080 78280263 78280080 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78279716 78279897 78279716 ID=MS.gene074488.t1.exon7;Parent=MS.gene074488.t1
chr5.4 CDS 78279716 78279897 78279716 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78279400 78279502 78279400 ID=MS.gene074488.t1.exon8;Parent=MS.gene074488.t1
chr5.4 CDS 78279400 78279502 78279400 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78278980 78279311 78278980 ID=MS.gene074488.t1.exon9;Parent=MS.gene074488.t1
chr5.4 CDS 78278980 78279311 78278980 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
chr5.4 exon 78278408 78278849 78278408 ID=MS.gene074488.t1.exon10;Parent=MS.gene074488.t1
chr5.4 CDS 78278408 78278849 78278408 ID=cds.MS.gene074488.t1;Parent=MS.gene074488.t1
Gene Sequence

>MS.gene074488

ATGCATACACCATCATCAGTGAAGAGAAAGAAGAATCACACACACACAGACAAGTCAAGAAGACTCATCGGACTCCTTCTTGCCAGTTTCCTTCGCCGTCGTCTCATCCATCGCCTCCTCATTTCCGCCATCTCCGTTTGCCTTCTCTTTCTTTTCATCATGCTCTCACTCTTCGCTCCTTCCCCTTTTATTGTAAATGCTCTTCATCCTTCTACTTCAACTACCTTCTATTTCATTTATAATATATGCTTTTCGTCATTGCATTCTTCTGTTCTGTGTGTAACTTTGATTGTATTTCAGGAAAATGATTTGGTTAAATCTCGTTTAGATTCTCATCAGCAGCAATCGCCATTTCACATTCCGGTAATCAAACAACCTTCTCACCTTTTTTTTTTTATGCATTTTGTGTTAGGTTGCTAATATCGATCTTCTTTTTGTTTATTGGAATTTGAAGGCAAATGAAGCTACTTACAGGCATGATCTATGGAATTCAAGAAACTCACAGCTTTTCTCTGCCTGTAGTAATGCCGGTGTCAACTTTGCAAGTAAACTTCATCCTAGACTCCCAGTCCTTTTTTCATCTCATGAATTGTAATTCCTATATTTAATTGCTTTTGATTGAGCGTATTTATTCATATTTCTAACTTTTGCAGAGGCTAATACCAAAACTCATCCGGATCGATACTTGTTAATCGCAACCAGTGGAGGCTTAAATCAACAGAGAACAGGGGTAATTAAGCTAAGCTTCTTTCTTAATGAACTGTGTCTGTGCTGTTGCTTTGTTTATGCAATCTTTTTAGTCAAGTAATGAATGTATAGAGCAGTCCAAGGAGGATAACTTATTAATCGGAATATGTACTTCTTTCTACATGTAACTAATTGTTTTATTTGAGTATGAATATTTCTTTCATTTGTTCAATTAGTAAACTTAATTGCATTCAAAATACTATTTTATATAGATATAGTGGCGACCGTGGTCATTTACAAAAAGTATAGGTTGAAATCTCTACCCAATAATTATAGGTCTGCAACAAATTGGTGATTTGCTATACAGATTTTGTGTGTCCCATTCCGCTAAAAAAACACAATTAGGATTTTCTTGATCGAATTTGACGAATGAGTGGTGATACGAATTTGAATGATATATTACGTTATGTGGATGATGCAATATTTCTGGTATTGAAAAATTATAGATTGTTTGTGGGTGCAAGAATTATAGGTCTGCAAAAATTAGTGATTTGATATACAAATTTTGTATGTGCCATTCGGCTAAAATAACTCAATTAGGATTTTCTAGATCAAATTTGATGAAGTGGATGCAATATTTCTGGCATTGAAAAAATACAGATTGTTTGTGGGTGCAGATAATTGATGCTGTTGTGGCTGCCTATCTTTTGAATGCTACTCTTGTTATTCCAGAATTGGATCATACATCTTTCTGGAAAGACAACAGGTCTGTGTTTACATTTCACTTCTATCATATCCAGGATGTCTCTTAAATCACATATAATATTTTCTTTGGTAGCAGAACACATTTGTCATTGTACTCCTTAATTTGTATGTAATATTTGATTATTATTTTTTTGCAGCAATTTTTCTCAATTATTTGTTGCCGATTGGTTTATAACATCCCTTCGGAATGACATAAGAGTTGTAAAGCAGCTACCAAATATGGGAGAGAAATTTGTGACTCCATATACAGTGCGTGTTCCAAGGAAATGCACCCCCAAATGCTACGAGGGTCGCGTTTTACCAGTGCTGGTCAAGAAGCGTGTAAGTGTCTAATTAAAGGTCCCTAATCGGAACCTTTCATCAAAATTATTTTCATGTATCCCTCAATTTTATTAATAAATTTTGTGGTTCCAACATTGTAAGTTAGGTATCCAACATACTAGTTATTGTAGCAAGTTGTGTTGATGCCATATGATTGTATAACATTTAATTGCATTACACAGGTTGTTCGGCTCACGAAGTTTGACTACAGACTTTCTAATCTGCTGGATGATGATCTACAAAAGCTGAGGTGCAGGGTAAACTACCATGCTCTCAAATTTACGGATTCGATTCAGGGAATGGGAAATTTGTTGGTTGAGCGGATGAGAATGAAAAGCATGCGTTTCATCGCGCTACATTTAAGGTAAGATTCCATTTGATAATTATAACTAAATATTTGTCCCTGGTTCCTTTGATATGCCATTCTTCAGCATGTGATCAGCTAAATCCTAAAACAATAATTTACATTTTTCAAATTGATACGAAAGCTCTAGGCCAACTTCAATTTCATTTTCTTTTCCGAAGTTCAATGTGATAATTGTTGATCCAAACTGCTTGTTTTTTCTGTTTCATATAGGTTTGAACCGGATATGCTTGCATTCTCTGGTTGCTATTATGGTGGGGGAGAAAAGGAAAGAAAAGAACTTGGTGAAATTAGGAAGCGGTGGAAAAATTTACATGTAAGGTTCTATTGACATTCAAGATATCTTTATTAACTGTATGAAAATTTGCATAATCTTACTATTTGATAATTAATGTGACTGACAGGCAAGCAATCCTGAGAAAGTAAGAAGACATGGAAGGTGTCCACTTACCCCAGAGGAAGTTGGTCTTATGCTTAGAGCACTAGGTTTTGGAATTGAAACGCATTTGTATGTGGCTTCTGGAGAAATATATGGAGGCGAGGAAACCCTAGCACCCCTCAGAGCTCTCTTCCCCAACTTCCATTCAAAGGAGACCTTAGCTACCAAGGAGGAATTGGCCCCATTTGTGTCTTTCTCTTCACGCATGGCTGCGCTTGATTACATAGTTTGTGATGACAGCGACGTTTTTGTAACAAACAACAATGGGAACATGGCTAAAATTCTAGCTGGAAGAAGGTAAGCTAGTTTATTAGGGCGTGAGATTCTTCTAGCTAGTCTCATGTGCTTAATTTATTCCAAGTAGTTGGTTTCTATTTGGACAGTACTTTTCCTACACATACTGATATTCAGACTTTCTCTGTTGTAGGAGGTACTTTGGTCATAAACCTACCATCCGCCCTAACGGAAAAAAGTTGAACCCCTTATTCATGAACAAGAATAACATGACATGGCAAGAATTTGCATCTCGAGTTCGAACTTTCCAGGTAGGCTTCATGGGAGAGCCTAATGAGTTGAGGTCAGGCAGTGGTGAGTTTCACGAGAATCCAACGTCTTGCATATGTCAAAACTCAGGGTCGGAGGTAAAAACTGAGGGATTTTCCAATCCTCAAAATCAAACGCATGAAGGCAATAAGGTAGAAAACAGGACAGAAAACCAGCCTGCTGAGGAAGTACGAGAGTGGTCTGAATTAGATTATGATTTGGACAATTATAGAAAGCAGGTTCAGCTCAAAGGAACTAATATTGAGTCGCTCCCCCTTCTAATGGGTACTGACCAAGCTGAAGTGCAAGTATTCTTCTCAGATTAG
Protein sequence

>MS.gene074488.t1

MHTPSSVKRKKNHTHTDKSRRLIGLLLASFLRRRLIHRLLISAISVCLLFLFIMLSLFAPSPFIENDLVKSRLDSHQQQSPFHIPANEATYRHDLWNSRNSQLFSACSNAGVNFAKANTKTHPDRYLLIATSGGLNQQRTGIIDAVVAAYLLNATLVIPELDHTSFWKDNSNFSQLFVADWFITSLRNDIRVVKQLPNMGEKFVTPYTVRVPRKCTPKCYEGRVLPVLVKKRVVRLTKFDYRLSNLLDDDLQKLRCRVNYHALKFTDSIQGMGNLLVERMRMKSMRFIALHLRFEPDMLAFSGCYYGGGEKERKELGEIRKRWKNLHASNPEKVRRHGRCPLTPEEVGLMLRALGFGIETHLYVASGEIYGGEETLAPLRALFPNFHSKETLATKEELAPFVSFSSRMAALDYIVCDDSDVFVTNNNGNMAKILAGRRRYFGHKPTIRPNGKKLNPLFMNKNNMTWQEFASRVRTFQVGFMGEPNELRSGSGEFHENPTSCICQNSGSEVKTEGFSNPQNQTHEGNKVENRTENQPAEEVREWSELDYDLDNYRKQVQLKGTNIESLPLLMGTDQAEVQVFFSD