Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074592.t1 | XP_003629390.1 | 66.7 | 123 | 23 | 3 | 3 | 125 | 56 | 160 | 1.60E-31 | 145.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074592.t1 | P17093 | 65.9 | 123 | 23 | 4 | 3 | 125 | 56 | 159 | 2.1e-32 | 139.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074592.t1 | B7FGL2 | 66.7 | 123 | 23 | 3 | 3 | 125 | 56 | 160 | 1.1e-31 | 145.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056978 | MS.gene074592 | 0.813673 | 2.27E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074592.t1 | MTR_8g076830 | 66.667 | 123 | 23 | 3 | 3 | 125 | 56 | 160 | 1.10e-47 | 150 |
| MS.gene074592.t1 | MTR_4g116410 | 65.854 | 123 | 24 | 3 | 3 | 125 | 56 | 160 | 2.42e-47 | 150 |
| MS.gene074592.t1 | MTR_1g017780 | 62.097 | 124 | 28 | 4 | 3 | 125 | 56 | 161 | 1.19e-43 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074592.t1 | AT3G48930 | 62.602 | 123 | 28 | 3 | 3 | 125 | 56 | 160 | 3.19e-43 | 139 |
| MS.gene074592.t1 | AT4G30800 | 60.976 | 123 | 29 | 4 | 3 | 125 | 56 | 159 | 2.78e-41 | 134 |
| MS.gene074592.t1 | AT5G23740 | 60.163 | 123 | 30 | 4 | 3 | 125 | 56 | 159 | 3.69e-40 | 131 |
Find 0 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAAAATAATATCAA+TGG | - | 57562:1429-1448 | MS.gene074592:intergenic | 10.0% |
| !! | ATAAAAAAAAAAACAGAAAT+TGG | - | 57562:1523-1542 | MS.gene074592:intergenic | 10.0% |
| !! | TAAAAAAAAAAACAGAAATT+GGG | - | 57562:1522-1541 | MS.gene074592:intergenic | 10.0% |
| !! | TAAAAAAAAAATGTAGTCTT+TGG | + | 57562:1888-1907 | MS.gene074592:intron | 15.0% |
| !! | TGTTTGTAAATAAATAATAG+TGG | + | 57562:2224-2243 | MS.gene074592:intron | 15.0% |
| !!! | AAAGACTACATTTTTTTTTT+AGG | - | 57562:1889-1908 | MS.gene074592:intergenic | 15.0% |
| !! | TAAAGCAAAATTGAGTTAAA+TGG | + | 57562:1943-1962 | MS.gene074592:intron | 20.0% |
| !! | TAAGTGTGCAAATAAAAAAT+GGG | - | 57562:2976-2995 | MS.gene074592:intergenic | 20.0% |
| !! | TATTTATTTACAAACACAAC+AGG | - | 57562:2220-2239 | MS.gene074592:intergenic | 20.0% |
| !! | TGTCTGTTTATATGATAAAT+TGG | + | 57562:2190-2209 | MS.gene074592:intron | 20.0% |
| !! | TTAATGGTTATGTTATGTTA+CGG | + | 57562:2376-2395 | MS.gene074592:intron | 20.0% |
| !!! | ATGTTCTAAAACTTACATAT+AGG | + | 57562:1729-1748 | MS.gene074592:intron | 20.0% |
| !!! | ATTTATGTATGTTTTGATAC+TGG | + | 57562:2066-2085 | MS.gene074592:intron | 20.0% |
| !!! | GAAGGTAATGTTTAATTTTA+GGG | + | 57562:1693-1712 | MS.gene074592:intron | 20.0% |
| !!! | GGCATTTTTTTTTATAAAAC+AGG | + | 57562:2779-2798 | MS.gene074592:intron | 20.0% |
| !!! | TGAAGGTAATGTTTAATTTT+AGG | + | 57562:1692-1711 | MS.gene074592:intron | 20.0% |
| ! | AAACTTACATATAGGAATGT+AGG | + | 57562:1737-1756 | MS.gene074592:intron | 25.0% |
| ! | AAATTAAACATTACCTTCAG+TGG | - | 57562:1691-1710 | MS.gene074592:intergenic | 25.0% |
| ! | AACTAAAACAAATACACTCA+AGG | - | 57562:1781-1800 | MS.gene074592:intergenic | 25.0% |
| ! | AACTTACATATAGGAATGTA+GGG | + | 57562:1738-1757 | MS.gene074592:intron | 25.0% |
| ! | AAGTGTGCAAATAAAAAATG+GGG | - | 57562:2975-2994 | MS.gene074592:intergenic | 25.0% |
| ! | AATTTCACTTTCAAAGTAAC+TGG | - | 57562:2522-2541 | MS.gene074592:intergenic | 25.0% |
| ! | ATTGAATTCCTTTCATACTA+TGG | + | 57562:2948-2967 | MS.gene074592:intron | 25.0% |
| ! | CTAAGTGTGCAAATAAAAAA+TGG | - | 57562:2977-2996 | MS.gene074592:intergenic | 25.0% |
| ! | TTAGATAAGGATAGAAACAT+TGG | + | 57562:1984-2003 | MS.gene074592:intron | 25.0% |
| ! | TTCTATAGAAAGCAAAAGAA+AGG | - | 57562:3156-3175 | MS.gene074592:intergenic | 25.0% |
| ! | TTTCTATCCTTATCTAATTG+TGG | - | 57562:1981-2000 | MS.gene074592:intergenic | 25.0% |
| !! | GCAATACTTATTCTAGTTAT+TGG | + | 57562:2268-2287 | MS.gene074592:intron | 25.0% |
| !! | GTATTGAGACATGTAATTTT+TGG | + | 57562:2353-2372 | MS.gene074592:intron | 25.0% |
| !!! | ACTTTATTAAGAAGTATCAG+AGG | + | 57562:2924-2943 | MS.gene074592:CDS | 25.0% |
| !!! | ATTTTAGTCCTCAAATGTAT+TGG | + | 57562:1805-1824 | MS.gene074592:intron | 25.0% |
| !!! | GACATGTAATTTTTGGTTAA+TGG | + | 57562:2360-2379 | MS.gene074592:intron | 25.0% |
| !!! | TTCTGTTTCTAATACAGAAA+TGG | + | 57562:2758-2777 | MS.gene074592:intron | 25.0% |
| ACACTTGCAAGACATAATAT+AGG | - | 57562:2449-2468 | MS.gene074591:intergenic | 30.0% | |
| ACAGAGGAGTAACAAAAAAA+GGG | - | 57562:3017-3036 | MS.gene074591:intergenic | 30.0% | |
| ACATACCATAGAAACACTTT+GGG | - | 57562:1487-1506 | MS.gene074591:intergenic | 30.0% | |
| ATGTTATTATTGGACAATGC+AGG | + | 57562:3111-3130 | MS.gene074592:CDS | 30.0% | |
| TACATACCATAGAAACACTT+TGG | - | 57562:1488-1507 | MS.gene074592:intergenic | 30.0% | |
| TAGAGTAGTTGCACAAATAA+AGG | - | 57562:3233-3252 | MS.gene074592:intergenic | 30.0% | |
| TATAGACCAATTCTTTCAAG+CGG | - | 57562:2405-2424 | MS.gene074592:intergenic | 30.0% | |
| TCCTCAAATGTATTGGATTT+GGG | + | 57562:1812-1831 | MS.gene074592:intron | 30.0% | |
| TGAATAGGACTATTATTGTC+AGG | + | 57562:2888-2907 | MS.gene074592:CDS | 30.0% | |
| TGCAAACAGTATAATGTTCT+CGG | + | 57562:2494-2513 | MS.gene074592:intron | 30.0% | |
| TGCATTGTCCAATAATAACA+TGG | - | 57562:3112-3131 | MS.gene074592:intergenic | 30.0% | |
| TTTATTTGTGCAACTACTCT+AGG | + | 57562:3232-3251 | MS.gene074592:CDS | 30.0% | |
| ! | ATTGTTAATAGCTCTGTGTA+TGG | + | 57562:2563-2582 | MS.gene074592:intron | 30.0% |
| AAGATACAGAGATAGAACGA+AGG | - | 57562:1398-1417 | MS.gene074591:intergenic | 35.0% | |
| AGTTCGAAGAAATCTGGTAA+GGG | + | 57562:1593-1612 | MS.gene074592:intron | 35.0% | |
| ATAGGACTATTATTGTCAGG+AGG | + | 57562:2891-2910 | MS.gene074592:CDS | 35.0% | |
| CCTCAAATGTATTGGATTTG+GGG | + | 57562:1813-1832 | MS.gene074592:intron | 35.0% | |
| GAAGGAGACCATGTTATTAT+TGG | + | 57562:3101-3120 | MS.gene074592:CDS | 35.0% | |
| GACAGAGGAGTAACAAAAAA+AGG | - | 57562:3018-3037 | MS.gene074592:intergenic | 35.0% | |
| GTCCTCAAATGTATTGGATT+TGG | + | 57562:1811-1830 | MS.gene074592:intron | 35.0% | |
| TCGTTCTATCTCTGTATCTT+TGG | + | 57562:1398-1417 | MS.gene074592:intron | 35.0% | |
| TGCAAAGCCACAATTAGATA+AGG | + | 57562:1971-1990 | MS.gene074592:intron | 35.0% | |
| TTCTTTGTTACAGACTGAGA+AGG | + | 57562:1447-1466 | MS.gene074592:intron | 35.0% | |
| ! | AATAGCTCTGTGTATGGTTA+CGG | + | 57562:2569-2588 | MS.gene074592:intron | 35.0% |
| ! | AATCGTTTCTGGAAATCAGT+TGG | + | 57562:1639-1658 | MS.gene074592:intron | 35.0% |
| ! | TATGTCTTGCAAGTGTTGTT+GGG | + | 57562:2453-2472 | MS.gene074592:intron | 35.0% |
| ! | TGTAGGGATAAACAGCATTT+TGG | + | 57562:1754-1773 | MS.gene074592:intron | 35.0% |
| ! | TTATGTCTTGCAAGTGTTGT+TGG | + | 57562:2452-2471 | MS.gene074592:intron | 35.0% |
| ! | TTTTTGTTACTCCTCTGTCT+AGG | + | 57562:3019-3038 | MS.gene074592:intron | 35.0% |
| !!! | ACGCTATTTCTTTTGCATAG+TGG | + | 57562:2023-2042 | MS.gene074592:intron | 35.0% |
| AATGGGGACCATAGTATGAA+AGG | - | 57562:2959-2978 | MS.gene074591:intergenic | 40.0% | |
| CAATGCAGGTGAGACTTTAT+AGG | + | 57562:3125-3144 | MS.gene074592:intron | 40.0% | |
| CAGTTCGAAGAAATCTGGTA+AGG | + | 57562:1592-1611 | MS.gene074592:intron | 40.0% | |
| CCCCAAATCCAATACATTTG+AGG | - | 57562:1816-1835 | MS.gene074592:intergenic | 40.0% | |
| GAAAAGGTGGAAATCGTTTC+TGG | + | 57562:1628-1647 | MS.gene074592:intron | 40.0% | |
| GTTCGAAGAAATCTGGTAAG+GGG | + | 57562:1594-1613 | MS.gene074592:intron | 40.0% | |
| TAATAGAGCCTCACATTCGA+GGG | + | 57562:1847-1866 | MS.gene074592:intron | 40.0% | |
| TCCTATTCATCTTAGCACTG+TGG | - | 57562:2877-2896 | MS.gene074592:intergenic | 40.0% | |
| TCTGTCTAGGTATGAGAAGA+GGG | + | 57562:3032-3051 | MS.gene074592:intron | 40.0% | |
| TGGACACTTCTTGTCAATGT+AGG | - | 57562:2807-2826 | MS.gene074592:intergenic | 40.0% | |
| TGTTGCAGTTCGAAGAAATC+TGG | + | 57562:1587-1606 | MS.gene074592:intron | 40.0% | |
| TTCGAAGAAATCTGGTAAGG+GGG | + | 57562:1595-1614 | MS.gene074592:intron | 40.0% | |
| ! | AAACGATTTCCACCTTTTCC+AGG | - | 57562:1627-1646 | MS.gene074592:intergenic | 40.0% |
| ! | AGCAACCCAAAGTGTTTCTA+TGG | + | 57562:1479-1498 | MS.gene074592:intron | 40.0% |
| ! | GTTTGTCTGAGATTCCTCTT+GGG | + | 57562:2115-2134 | MS.gene074592:intron | 40.0% |
| ! | TGTTTGTCTGAGATTCCTCT+TGG | + | 57562:2114-2133 | MS.gene074592:intron | 40.0% |
| ! | TTCTGGAAATCAGTTGGTCT+TGG | + | 57562:1645-1664 | MS.gene074592:intron | 40.0% |
| ATCACCTTGCTTCCGTGTTA+AGG | + | 57562:3079-3098 | MS.gene074592:CDS | 45.0% | |
| ATCTAGTGGTGCAGGAAAGA+AGG | + | 57562:3308-3327 | MS.gene074592:CDS | 45.0% | |
| ATGGAAACATTGCCGGTGAA+TGG | - | 57562:2827-2846 | MS.gene074592:intergenic | 45.0% | |
| ATTACCTTCAGTGGCTTCTC+TGG | - | 57562:1682-1701 | MS.gene074592:intergenic | 45.0% | |
| CATGGTCTCCTTCCTTAACA+CGG | - | 57562:3094-3113 | MS.gene074592:intergenic | 45.0% | |
| CGGTATCCGCTTGAAAGAAT+TGG | + | 57562:2396-2415 | MS.gene074592:intron | 45.0% | |
| CTCTGTCTAGGTATGAGAAG+AGG | + | 57562:3031-3050 | MS.gene074592:intron | 45.0% | |
| CTCTTCTCATACCTAGACAG+AGG | - | 57562:3033-3052 | MS.gene074592:intergenic | 45.0% | |
| GATTCCTCTTGGGCAATATC+AGG | + | 57562:2125-2144 | MS.gene074592:intron | 45.0% | |
| GCCACAGTGCTAAGATGAAT+AGG | + | 57562:2873-2892 | MS.gene074592:CDS | 45.0% | |
| GTAATAGAGCCTCACATTCG+AGG | + | 57562:1846-1865 | MS.gene074592:intron | 45.0% | |
| TTACCTTCAGTGGCTTCTCT+GGG | - | 57562:1681-1700 | MS.gene074592:intergenic | 45.0% | |
| ! | GCAGGAAAGAAGGCTTTTAC+TGG | + | 57562:3318-3337 | MS.gene074592:CDS | 45.0% |
| ! | TCTTGCAAGTGTTGTTGGGA+AGG | + | 57562:2457-2476 | MS.gene074592:intron | 45.0% |
| !! | CTGAGCGTCTAGCTTTGTAT+TGG | + | 57562:2089-2108 | MS.gene074592:intron | 45.0% |
| !! | GACAAGAAGTGTCCATTCAC+CGG | + | 57562:2812-2831 | MS.gene074592:intron | 45.0% |
| AACCTCACAGTCTTGGAGAG+TGG | - | 57562:3258-3277 | MS.gene074591:intergenic | 50.0% | |
| AAGCAAGGTGATACGTGAGC+GGG | - | 57562:3071-3090 | MS.gene074591:intergenic | 50.0% | |
| ATCCCAGCTGGATCATCTAG+TGG | + | 57562:3294-3313 | MS.gene074592:CDS | 50.0% | |
| CACCACTAGATGATCCAGCT+GGG | - | 57562:3299-3318 | MS.gene074592:intergenic | 50.0% | |
| CACGTTGAACCTCACAGTCT+TGG | - | 57562:3265-3284 | MS.gene074592:intergenic | 50.0% | |
| CCTTCCTTAACACGGAAGCA+AGG | - | 57562:3086-3105 | MS.gene074592:intergenic | 50.0% | |
| CCTTGCTTCCGTGTTAAGGA+AGG | + | 57562:3083-3102 | MS.gene074592:CDS | 50.0% | |
| CTTAGCACTGTGGCAAGTTC+CGG | - | 57562:2867-2886 | MS.gene074592:intergenic | 50.0% | |
| GAAATCTGGTAAGGGGGAAG+AGG | + | 57562:1601-1620 | MS.gene074592:intron | 50.0% | |
| GAAGCAAGGTGATACGTGAG+CGG | - | 57562:3072-3091 | MS.gene074592:intergenic | 50.0% | |
| GTGTTGAAAGTGATCCCAGC+TGG | + | 57562:3282-3301 | MS.gene074592:CDS | 50.0% | |
| GTTATGCTCCCTCGAATGTG+AGG | - | 57562:1858-1877 | MS.gene074592:intergenic | 50.0% | |
| TACCTTCAGTGGCTTCTCTG+GGG | - | 57562:1680-1699 | MS.gene074592:intergenic | 50.0% | |
| TCACCGGCAATGTTTCCATC+CGG | + | 57562:2828-2847 | MS.gene074592:CDS | 50.0% | |
| TCCTCTTGGGCAATATCAGG+TGG | + | 57562:2128-2147 | MS.gene074592:intron | 50.0% | |
| ACCCCGGATGGAAACATTGC+CGG | - | 57562:2834-2853 | MS.gene074592:intergenic | 55.0% | |
| ACCGGCAATGTTTCCATCCG+GGG | + | 57562:2830-2849 | MS.gene074592:CDS | 55.0% | |
| ACCTTCAGTGGCTTCTCTGG+GGG | - | 57562:1679-1698 | MS.gene074592:intergenic | 55.0% | |
| ATCCGGGGTCGTATCTTAGC+CGG | + | 57562:2845-2864 | MS.gene074592:CDS | 55.0% | |
| CACCGGCAATGTTTCCATCC+GGG | + | 57562:2829-2848 | MS.gene074592:CDS | 55.0% | |
| GCACCACTAGATGATCCAGC+TGG | - | 57562:3300-3319 | MS.gene074592:intergenic | 55.0% | |
| GCCACCTGATATTGCCCAAG+AGG | - | 57562:2132-2151 | MS.gene074592:intergenic | 55.0% | |
| GCTGGATCATCTAGTGGTGC+AGG | + | 57562:3300-3319 | MS.gene074592:CDS | 55.0% | |
| TTCCGGCTAAGATACGACCC+CGG | - | 57562:2850-2869 | MS.gene074592:intergenic | 55.0% | |
| ACCCCCAGAGAAGCCACTGA+AGG | + | 57562:1675-1694 | MS.gene074592:intron | 60.0% | |
| AGGGGGAAGAGGCCTGGAAA+AGG | + | 57562:1612-1631 | MS.gene074592:intron | 60.0% | |
| GGCCACTCTCCAAGACTGTG+AGG | + | 57562:3253-3272 | MS.gene074592:CDS | 60.0% | |
| GGCTAAGATACGACCCCGGA+TGG | - | 57562:2846-2865 | MS.gene074592:intergenic | 60.0% | |
| GGGAAGAGGCCTGGAAAAGG+TGG | + | 57562:1615-1634 | MS.gene074592:intron | 60.0% | |
| CTGGTAAGGGGGAAGAGGCC+TGG | + | 57562:1606-1625 | MS.gene074592:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57562 | gene | 1363 | 3347 | 1363 | ID=MS.gene074592 |
| 57562 | mRNA | 1363 | 3347 | 1363 | ID=MS.gene074592.t1;Parent=MS.gene074592 |
| 57562 | exon | 1363 | 1374 | 1363 | ID=MS.gene074592.t1.exon1;Parent=MS.gene074592.t1 |
| 57562 | CDS | 1363 | 1374 | 1363 | ID=cds.MS.gene074592.t1;Parent=MS.gene074592.t1 |
| 57562 | exon | 2818 | 2945 | 2818 | ID=MS.gene074592.t1.exon2;Parent=MS.gene074592.t1 |
| 57562 | CDS | 2818 | 2945 | 2818 | ID=cds.MS.gene074592.t1;Parent=MS.gene074592.t1 |
| 57562 | exon | 3041 | 3132 | 3041 | ID=MS.gene074592.t1.exon3;Parent=MS.gene074592.t1 |
| 57562 | CDS | 3041 | 3132 | 3041 | ID=cds.MS.gene074592.t1;Parent=MS.gene074592.t1 |
| 57562 | exon | 3202 | 3347 | 3202 | ID=MS.gene074592.t1.exon4;Parent=MS.gene074592.t1 |
| 57562 | CDS | 3202 | 3347 | 3202 | ID=cds.MS.gene074592.t1;Parent=MS.gene074592.t1 |
| Gene Sequence |
| Protein sequence |
