Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07468.t1 | XP_030957732.1 | 47.3 | 55 | 25 | 1 | 1 | 55 | 81 | 131 | 6.80E-06 | 59.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07468.t1 | Q8W4D0 | 70.0 | 30 | 9 | 0 | 1 | 30 | 92 | 121 | 4.5e-06 | 51.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052609 | MS.gene07468 | 0.810182 | 1.31E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTGAGAATTGCAAAATTA+TGG | 0.264422 | 1.3:+55364282 | MS.gene07468:CDS |
| TTTCAGGGAATTTGAAGTTT+TGG | 0.308451 | 1.3:+55363226 | None:intergenic |
| TCTTGGTCCTATTGAAGGTC+TGG | 0.363083 | 1.3:+55363296 | MS.gene07468:CDS |
| TTTGCAAACTCAATATCAAT+TGG | 0.420851 | 1.3:-55363258 | None:intergenic |
| TCTTATCTTGGTCCTATTGA+AGG | 0.428550 | 1.3:+55363291 | MS.gene07468:CDS |
| AAACAATTCAGATCTTATCT+TGG | 0.434631 | 1.3:+55363279 | MS.gene07468:CDS |
| CTTACAACCAGACCTTCAAT+AGG | 0.468380 | 1.3:-55363303 | None:intergenic |
| ATTATGGCACTGTTTAGTCT+CGG | 0.582376 | 1.3:+55364298 | MS.gene07468:CDS |
| CATGTAAATGAGACTCATTG+TGG | 0.759595 | 1.3:+55364256 | MS.gene07468:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTTATATGATTTAATC+TGG | + | chr1.3:55363364-55363383 | MS.gene07468:intron | 10.0% |
| !!! | AATTTGAAATTTTGAATTTA+TGG | + | chr1.3:55363695-55363714 | MS.gene07468:intron | 10.0% |
| !! | TATTCTATTCATTAACATAG+CGG | - | chr1.3:55363486-55363505 | None:intergenic | 20.0% |
| !!! | TTTTACTGTTACACAAAAAA+GGG | - | chr1.3:55363347-55363366 | None:intergenic | 20.0% |
| !!! | TTTTTACTGTTACACAAAAA+AGG | - | chr1.3:55363348-55363367 | None:intergenic | 20.0% |
| ! | AAACAATTCAGATCTTATCT+TGG | + | chr1.3:55363279-55363298 | MS.gene07468:CDS | 25.0% |
| ! | AATATCCAAACTGAATGTAA+AGG | + | chr1.3:55363590-55363609 | MS.gene07468:intron | 25.0% |
| ! | AGATATGTTGTTAAGTGATT+AGG | + | chr1.3:55363858-55363877 | MS.gene07468:intron | 25.0% |
| ! | AGTTGAGAATTGCAAAATTA+TGG | + | chr1.3:55364282-55364301 | MS.gene07468:CDS | 25.0% |
| ! | ATATGTTGTTAAGTGATTAG+GGG | + | chr1.3:55363860-55363879 | MS.gene07468:intron | 25.0% |
| ! | GATATGTTGTTAAGTGATTA+GGG | + | chr1.3:55363859-55363878 | MS.gene07468:intron | 25.0% |
| ! | TATTTCCTTTACATTCAGTT+TGG | - | chr1.3:55363598-55363617 | None:intergenic | 25.0% |
| ! | TTTGCAAACTCAATATCAAT+TGG | - | chr1.3:55363261-55363280 | None:intergenic | 25.0% |
| !!! | AATCTTTTGTCTTTTGTTGT+TGG | + | chr1.3:55364083-55364102 | MS.gene07468:intron | 25.0% |
| !!! | ATTTGAAGTTTTGGAAGAAA+AGG | + | chr1.3:55363235-55363254 | MS.gene07468:CDS | 25.0% |
| !!! | TTCTAGTTTTCAAATCTCTA+TGG | + | chr1.3:55363893-55363912 | MS.gene07468:intron | 25.0% |
| AAATGCGTACTTACTTTCAA+TGG | - | chr1.3:55363971-55363990 | None:intergenic | 30.0% | |
| ACATTCAAAAGAAGGAATGA+AGG | - | chr1.3:55363999-55364018 | None:intergenic | 30.0% | |
| GATGTATGATAGTATCTATG+TGG | + | chr1.3:55363399-55363418 | MS.gene07468:intron | 30.0% | |
| TATTCGTTTCGTTATGCATT+TGG | + | chr1.3:55363658-55363677 | MS.gene07468:intron | 30.0% | |
| ! | TTTTGAATTTATGGTATCGC+AGG | + | chr1.3:55363704-55363723 | MS.gene07468:intron | 30.0% |
| ATGTAGAAAACAACCATCCA+TGG | - | chr1.3:55363937-55363956 | None:intergenic | 35.0% | |
| ATTATGGCACTGTTTAGTCT+CGG | + | chr1.3:55364298-55364317 | MS.gene07468:CDS | 35.0% | |
| CATGTAAATGAGACTCATTG+TGG | + | chr1.3:55364256-55364275 | MS.gene07468:CDS | 35.0% | |
| CTAGGACAACATTCAAAAGA+AGG | - | chr1.3:55364007-55364026 | None:intergenic | 35.0% | |
| GATAGTATCTATGTGGTCAT+TGG | + | chr1.3:55363406-55363425 | MS.gene07468:intron | 35.0% | |
| TCTTATCTTGGTCCTATTGA+AGG | + | chr1.3:55363291-55363310 | MS.gene07468:CDS | 35.0% | |
| TGACCTTCAAAACACTATAG+CGG | + | chr1.3:55363440-55363459 | MS.gene07468:intron | 35.0% | |
| TGTAGAAAACAACCATCCAT+GGG | - | chr1.3:55363936-55363955 | None:intergenic | 35.0% | |
| TTTAGTCTCGGTGAAACTTA+GGG | + | chr1.3:55364310-55364329 | MS.gene07468:CDS | 35.0% | |
| ! | TTGTTGTTGGTTGTGAAAGT+AGG | + | chr1.3:55364096-55364115 | MS.gene07468:intron | 35.0% |
| AAAACACTATAGCGGCATAG+AGG | + | chr1.3:55363448-55363467 | MS.gene07468:intron | 40.0% | |
| AAACACTATAGCGGCATAGA+GGG | + | chr1.3:55363449-55363468 | MS.gene07468:intron | 40.0% | |
| ACACAAAAAAGGGTTGTGTC+AGG | - | chr1.3:55363337-55363356 | None:intergenic | 40.0% | |
| AGTATCTATGTGGTCATTGG+CGG | + | chr1.3:55363409-55363428 | MS.gene07468:intron | 40.0% | |
| ATCTCTATGGATTTCGCTGA+TGG | + | chr1.3:55363906-55363925 | MS.gene07468:intron | 40.0% | |
| CATTTGCAGTTGCAACAATG+TGG | - | chr1.3:55364047-55364066 | None:intergenic | 40.0% | |
| CTTACAACCAGACCTTCAAT+AGG | - | chr1.3:55363306-55363325 | None:intergenic | 40.0% | |
| GAAGAAGAAGTAGAGAGTGA+AGG | + | chr1.3:55363793-55363812 | MS.gene07468:intron | 40.0% | |
| GAGGAGACAACAATAAAGCT+AGG | - | chr1.3:55364025-55364044 | None:intergenic | 40.0% | |
| GCGTACTTACTTTCAATGGA+AGG | - | chr1.3:55363967-55363986 | None:intergenic | 40.0% | |
| GTTTAGTCTCGGTGAAACTT+AGG | + | chr1.3:55364309-55364328 | MS.gene07468:CDS | 40.0% | |
| TTTATGGTATCGCAGGATGA+AGG | + | chr1.3:55363711-55363730 | MS.gene07468:intron | 40.0% | |
| !! | ATGCCGCTATAGTGTTTTGA+AGG | - | chr1.3:55363446-55363465 | None:intergenic | 40.0% |
| AAGTAGAGAGTGAAGGTGAG+AGG | + | chr1.3:55363800-55363819 | MS.gene07468:intron | 45.0% | |
| AGAAGTTGAAGGACAAGGAG+AGG | + | chr1.3:55363768-55363787 | MS.gene07468:intron | 45.0% | |
| AGTAGAGAGTGAAGGTGAGA+GGG | + | chr1.3:55363801-55363820 | MS.gene07468:intron | 45.0% | |
| CAGTTCCACTTCCACTAAAC+CGG | - | chr1.3:55364123-55364142 | None:intergenic | 45.0% | |
| GTTGGTTGTGAAAGTAGGAC+CGG | + | chr1.3:55364101-55364120 | MS.gene07468:intron | 45.0% | |
| TAAGGAGAGGGAGAAGTTGA+AGG | + | chr1.3:55363757-55363776 | MS.gene07468:intron | 45.0% | |
| TCTTGGTCCTATTGAAGGTC+TGG | + | chr1.3:55363296-55363315 | MS.gene07468:CDS | 45.0% | |
| TTGCAGTTGCAACAATGTGG+AGG | - | chr1.3:55364044-55364063 | None:intergenic | 45.0% | |
| ! | TGAAAGTAGGACCGGTTTAG+TGG | + | chr1.3:55364109-55364128 | MS.gene07468:intron | 45.0% |
| AGGGCAAGAAGGTTAAGGAG+AGG | + | chr1.3:55363744-55363763 | MS.gene07468:intron | 50.0% | |
| ATGGTATCGCAGGATGAAGG+AGG | + | chr1.3:55363714-55363733 | MS.gene07468:intron | 50.0% | |
| CGGTTTAGTGGAAGTGGAAC+TGG | + | chr1.3:55364121-55364140 | MS.gene07468:intron | 50.0% | |
| GAGGGAGAAGTTGAAGGACA+AGG | + | chr1.3:55363763-55363782 | MS.gene07468:intron | 50.0% | |
| GGGCAAGAAGGTTAAGGAGA+GGG | + | chr1.3:55363745-55363764 | MS.gene07468:intron | 50.0% | |
| TATAGCGGCATAGAGGGTTG+TGG | + | chr1.3:55363455-55363474 | MS.gene07468:intron | 50.0% | |
| TGGTATCGCAGGATGAAGGA+GGG | + | chr1.3:55363715-55363734 | MS.gene07468:intron | 50.0% | |
| ! | ATGTGGTCATTGGCGGATAG+TGG | + | chr1.3:55363416-55363435 | MS.gene07468:intron | 50.0% |
| ! | TAGGACCGGTTTAGTGGAAG+TGG | + | chr1.3:55364115-55364134 | MS.gene07468:intron | 50.0% |
| ! | TTTCGCTGATGGTGAACCCA+TGG | + | chr1.3:55363917-55363936 | MS.gene07468:intron | 50.0% |
| AGAGAGTGAAGGTGAGAGGG+AGG | + | chr1.3:55363804-55363823 | MS.gene07468:intron | 55.0% | |
| AGTGAAGGTGAGAGGGAGGT+TGG | + | chr1.3:55363808-55363827 | MS.gene07468:intron | 55.0% | |
| ATCGCAGGATGAAGGAGGGT+GGG | + | chr1.3:55363719-55363738 | MS.gene07468:intron | 55.0% | |
| ATCGGAGAGGGGAAGAAGAG+AGG | + | chr1.3:55363833-55363852 | MS.gene07468:intron | 55.0% | |
| CGGCATAGAGGGTTGTGGTT+TGG | + | chr1.3:55363460-55363479 | MS.gene07468:intron | 55.0% | |
| GGGGTAGGGCAAGAAGGTTA+AGG | + | chr1.3:55363739-55363758 | MS.gene07468:intron | 55.0% | |
| TATCGCAGGATGAAGGAGGG+TGG | + | chr1.3:55363718-55363737 | MS.gene07468:intron | 55.0% | |
| TCGGAGAGGGGAAGAAGAGA+GGG | + | chr1.3:55363834-55363853 | MS.gene07468:intron | 55.0% | |
| ! | GCTGATGGTGAACCCATGGA+TGG | + | chr1.3:55363921-55363940 | MS.gene07468:intron | 55.0% |
| !! | GTGAGAGGGAGGTTGGTGAT+CGG | + | chr1.3:55363815-55363834 | MS.gene07468:intron | 55.0% |
| AGGATGAAGGAGGGTGGGGT+AGG | + | chr1.3:55363724-55363743 | MS.gene07468:intron | 60.0% | |
| GGATGAAGGAGGGTGGGGTA+GGG | + | chr1.3:55363725-55363744 | MS.gene07468:intron | 60.0% | |
| TCGCAGGATGAAGGAGGGTG+GGG | + | chr1.3:55363720-55363739 | MS.gene07468:intron | 60.0% | |
| !! | AGGGAGGTTGGTGATCGGAG+AGG | + | chr1.3:55363820-55363839 | MS.gene07468:intron | 60.0% |
| !! | GGAGGTTGGTGATCGGAGAG+GGG | + | chr1.3:55363822-55363841 | MS.gene07468:intron | 60.0% |
| !! | GGGAGGTTGGTGATCGGAGA+GGG | + | chr1.3:55363821-55363840 | MS.gene07468:intron | 60.0% |
| GAGGGTGGGGTAGGGCAAGA+AGG | + | chr1.3:55363733-55363752 | MS.gene07468:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 55363232 | 55364330 | 55363232 | ID=MS.gene07468 |
| chr1.3 | mRNA | 55363232 | 55364330 | 55363232 | ID=MS.gene07468.t1;Parent=MS.gene07468 |
| chr1.3 | exon | 55363232 | 55363320 | 55363232 | ID=MS.gene07468.t1.exon1;Parent=MS.gene07468.t1 |
| chr1.3 | CDS | 55363232 | 55363320 | 55363232 | ID=cds.MS.gene07468.t1;Parent=MS.gene07468.t1 |
| chr1.3 | exon | 55364241 | 55364330 | 55364241 | ID=MS.gene07468.t1.exon2;Parent=MS.gene07468.t1 |
| chr1.3 | CDS | 55364241 | 55364330 | 55364241 | ID=cds.MS.gene07468.t1;Parent=MS.gene07468.t1 |
| Gene Sequence |
| Protein sequence |
