Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08404.t1 | XP_013457403.1 | 95.7 | 115 | 5 | 0 | 1 | 115 | 1 | 115 | 3.20E-47 | 197.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08404.t1 | A0A072UNP9 | 95.7 | 115 | 5 | 0 | 1 | 115 | 1 | 115 | 2.3e-47 | 197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060429 | MS.gene08404 | 0.810241 | 1.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08404.t1 | MTR_4g094782 | 95.652 | 115 | 5 | 0 | 1 | 115 | 1 | 115 | 8.28e-57 | 172 |
| MS.gene08404.t1 | MTR_4g094782 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.46e-28 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGCTGCTCCTAAGCTTC+AGG | 0.303181 | 4.1:+17607800 | MS.gene08404:CDS |
| TTGAAGCCTGTTGGTAGAAT+AGG | 0.330969 | 4.1:+17608807 | MS.gene08404:CDS |
| TTCTTCCCTATTCTACCAAC+AGG | 0.374239 | 4.1:-17608813 | None:intergenic |
| CATATGTGACGCTTTCTACT+TGG | 0.404049 | 4.1:-17608891 | None:intergenic |
| TGAAGCCTGTTGGTAGAATA+GGG | 0.429770 | 4.1:+17608808 | MS.gene08404:CDS |
| TATTCTACCAACAGGCTTCA+AGG | 0.434559 | 4.1:-17608805 | None:intergenic |
| CATGGCGTTGTTGTTGATAG+TGG | 0.439825 | 4.1:-17607862 | None:intergenic |
| GCTTCTTTCTTGTCATAGAA+AGG | 0.440776 | 4.1:-17607750 | None:intergenic |
| ACTTCCATGGAAGTGGTTGA+TGG | 0.472045 | 4.1:-17607831 | None:intergenic |
| CGTCCCATCAACCACTTCCA+TGG | 0.478820 | 4.1:+17607827 | MS.gene08404:CDS |
| GGGACGGAAACCTGAAGCTT+AGG | 0.481913 | 4.1:-17607810 | None:intergenic |
| CTTCCATGGAAGTGGTTGAT+GGG | 0.520177 | 4.1:-17607830 | None:intergenic |
| AAGAAGTTTAAAATGGCTAA+AGG | 0.520775 | 4.1:+17608843 | MS.gene08404:CDS |
| AGTGGAAGTGGAAACTTCCA+TGG | 0.536646 | 4.1:-17607844 | None:intergenic |
| TGAGATCGAAGAGGGAGAAG+AGG | 0.542016 | 4.1:+17607697 | MS.gene08404:CDS |
| CAAGATGTGGAGATGGCTGA+TGG | 0.544852 | 4.1:+17608765 | MS.gene08404:intron |
| AAGACTTCCTTGAAGCCTGT+TGG | 0.560642 | 4.1:+17608798 | MS.gene08404:CDS |
| CACTATCAACAACAACGCCA+TGG | 0.564112 | 4.1:+17607863 | MS.gene08404:CDS |
| CATGGAAGTGGTTGATGGGA+CGG | 0.567913 | 4.1:-17607826 | None:intergenic |
| GTTGTTGTTGATAGTGGAAG+TGG | 0.580441 | 4.1:-17607856 | None:intergenic |
| GCTAAAGGCAACCGCCGCAA+TGG | 0.582224 | 4.1:+17608858 | MS.gene08404:CDS |
| AGTGGAAACTTCCATGGAAG+TGG | 0.602761 | 4.1:-17607838 | None:intergenic |
| GAAGTCAATGAGATCGAAGA+GGG | 0.602910 | 4.1:+17607689 | MS.gene08404:CDS |
| ACTATCAACAACAACGCCAT+GGG | 0.608842 | 4.1:+17607864 | MS.gene08404:CDS |
| CGAAGTCAATGAGATCGAAG+AGG | 0.658126 | 4.1:+17607688 | MS.gene08404:CDS |
| TTGGCTTTGAATTACCATTG+CGG | 0.667230 | 4.1:-17608872 | None:intergenic |
| GCTTTGAATTACCATTGCGG+CGG | 0.670430 | 4.1:-17608869 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAAATAAATATAAAAT+TGG | - | chr4.1:17608679-17608698 | None:intergenic | 0.0% |
| !! | ATTAAAATAAATATAAAATT+GGG | - | chr4.1:17608678-17608697 | None:intergenic | 0.0% |
| !!! | ACATTTACAATATTTTAAAT+AGG | + | chr4.1:17608012-17608031 | MS.gene08404:intron | 10.0% |
| !!! | TATTTATTTTAATTACGAAT+TGG | + | chr4.1:17608685-17608704 | MS.gene08404:intron | 10.0% |
| !! | AGTAATAATATATCTATCTA+TGG | - | chr4.1:17608206-17608225 | None:intergenic | 15.0% |
| !! | ATTGAAAAAGAAGTTTAAAA+TGG | + | chr4.1:17608836-17608855 | MS.gene08404:CDS | 15.0% |
| !!! | TTTTTCTTTAATTTCATAGT+TGG | + | chr4.1:17608536-17608555 | MS.gene08404:intron | 15.0% |
| !! | AACTACTAAAACAATAAAAG+TGG | - | chr4.1:17608118-17608137 | None:intergenic | 20.0% |
| !! | AATATATCTATCTATGGAAT+TGG | - | chr4.1:17608200-17608219 | None:intergenic | 20.0% |
| !! | ACCACAAAATGAAATAAAAA+AGG | - | chr4.1:17607901-17607920 | None:intergenic | 20.0% |
| !! | ATATATCTATCTATGGAATT+GGG | - | chr4.1:17608199-17608218 | None:intergenic | 20.0% |
| !! | TGAAATAAAAAAGGAAAAAG+TGG | - | chr4.1:17607892-17607911 | None:intergenic | 20.0% |
| !!! | TCCTTTTTTATTTCATTTTG+TGG | + | chr4.1:17607897-17607916 | MS.gene08404:intron | 20.0% |
| ! | AAAAACCCAATTCAATTTGA+AGG | - | chr4.1:17608382-17608401 | None:intergenic | 25.0% |
| ! | AAAACCCAATTCAATTTGAA+GGG | - | chr4.1:17608381-17608400 | None:intergenic | 25.0% |
| ! | ATCATAACAATAGCTTCAAA+AGG | - | chr4.1:17608482-17608501 | None:intergenic | 25.0% |
| ! | ATTTACCTAGTACAAATGTA+GGG | + | chr4.1:17607977-17607996 | MS.gene08404:intron | 25.0% |
| ! | TATTTACCTAGTACAAATGT+AGG | + | chr4.1:17607976-17607995 | MS.gene08404:intron | 25.0% |
| ! | TTCAAAAGGAAAAACAACAA+AGG | - | chr4.1:17608602-17608621 | None:intergenic | 25.0% |
| ! | TTTAATTACGAATTGGCATT+TGG | + | chr4.1:17608692-17608711 | MS.gene08404:intron | 25.0% |
| !! | AAGAAGTTTAAAATGGCTAA+AGG | + | chr4.1:17608843-17608862 | MS.gene08404:CDS | 25.0% |
| !!! | AAAACAGAAATGGGTTTTTA+GGG | - | chr4.1:17608451-17608470 | None:intergenic | 25.0% |
| !!! | GTTTTTGAATTTTCAAGATG+TGG | + | chr4.1:17608752-17608771 | MS.gene08404:intron | 25.0% |
| ACAATAAAAGTGGAGAACAA+AGG | - | chr4.1:17608108-17608127 | None:intergenic | 30.0% | |
| GAAAGAGAAAGAGAAAAAGA+AGG | - | chr4.1:17608345-17608364 | None:intergenic | 30.0% | |
| ! | GTGTTATGTCTTTAGTTTAG+CGG | + | chr4.1:17607919-17607938 | MS.gene08404:intron | 30.0% |
| ! | TGCATCACTCATTTTCTATT+GGG | - | chr4.1:17608062-17608081 | None:intergenic | 30.0% |
| ! | TTGCATCACTCATTTTCTAT+TGG | - | chr4.1:17608063-17608082 | None:intergenic | 30.0% |
| ! | TTTCATAGTTGGAGTTTTCT+TGG | + | chr4.1:17608547-17608566 | MS.gene08404:intron | 30.0% |
| !! | GAAAACAGAAATGGGTTTTT+AGG | - | chr4.1:17608452-17608471 | None:intergenic | 30.0% |
| !!! | CTTGTTTGTGTTGTTTGTTT+TGG | - | chr4.1:17608241-17608260 | None:intergenic | 30.0% |
| !!! | TTGTGTTGTTTGTTTTGGAA+GGG | - | chr4.1:17608236-17608255 | None:intergenic | 30.0% |
| !!! | TTTGTGTTGTTTGTTTTGGA+AGG | - | chr4.1:17608237-17608256 | None:intergenic | 30.0% |
| AACAACACAAACAAGAAGCT+TGG | + | chr4.1:17608246-17608265 | MS.gene08404:intron | 35.0% | |
| AACAATAGCTTCAAAAGGCT+TGG | - | chr4.1:17608477-17608496 | None:intergenic | 35.0% | |
| GCTTCTTTCTTGTCATAGAA+AGG | - | chr4.1:17607753-17607772 | None:intergenic | 35.0% | |
| GCTTGGAAGAAAACAGAAAT+GGG | - | chr4.1:17608460-17608479 | None:intergenic | 35.0% | |
| !! | GAATTTTCAAGATGTGGAGA+TGG | + | chr4.1:17608758-17608777 | MS.gene08404:intron | 35.0% |
| !! | TTGGCTTTGAATTACCATTG+CGG | - | chr4.1:17608875-17608894 | None:intergenic | 35.0% |
| !! | TTTTGAAAGATCTGTCACAG+TGG | - | chr4.1:17608411-17608430 | None:intergenic | 35.0% |
| !!! | CACTGATTTTGTTTTTGCTG+GGG | + | chr4.1:17608570-17608589 | MS.gene08404:intron | 35.0% |
| !!! | GCACTGATTTTGTTTTTGCT+GGG | + | chr4.1:17608569-17608588 | MS.gene08404:intron | 35.0% |
| AAAGGAAAAAGTGGTACCCA+TGG | - | chr4.1:17607883-17607902 | None:intergenic | 40.0% | |
| ACAAGAAGCTTGGAACTTGA+AGG | + | chr4.1:17608256-17608275 | MS.gene08404:intron | 40.0% | |
| ACCCAATTCAATTTGAAGGG+AGG | - | chr4.1:17608378-17608397 | None:intergenic | 40.0% | |
| ACTATCAACAACAACGCCAT+GGG | + | chr4.1:17607864-17607883 | MS.gene08404:CDS | 40.0% | |
| CATATGTGACGCTTTCTACT+TGG | - | chr4.1:17608894-17608913 | None:intergenic | 40.0% | |
| CCCAATTCAATTTGAAGGGA+GGG | - | chr4.1:17608377-17608396 | None:intergenic | 40.0% | |
| CCCTCCCTTCAAATTGAATT+GGG | + | chr4.1:17608374-17608393 | MS.gene08404:intron | 40.0% | |
| CTTTGATGAAAAGGAGTTGG+CGG | - | chr4.1:17608284-17608303 | None:intergenic | 40.0% | |
| GAAGTCAATGAGATCGAAGA+GGG | + | chr4.1:17607689-17607708 | MS.gene08404:CDS | 40.0% | |
| GGCTTGGAAGAAAACAGAAA+TGG | - | chr4.1:17608461-17608480 | None:intergenic | 40.0% | |
| TAATCACTGCCCTACTCTTT+CGG | - | chr4.1:17608149-17608168 | None:intergenic | 40.0% | |
| TATTCTACCAACAGGCTTCA+AGG | - | chr4.1:17608808-17608827 | None:intergenic | 40.0% | |
| TCCCTCCCTTCAAATTGAAT+TGG | + | chr4.1:17608373-17608392 | MS.gene08404:intron | 40.0% | |
| TGCTACCCTACATTTGTACT+AGG | - | chr4.1:17607985-17608004 | None:intergenic | 40.0% | |
| TTCTTCCCTATTCTACCAAC+AGG | - | chr4.1:17608816-17608835 | None:intergenic | 40.0% | |
| ! | CCAACTCCTTTTCATCAAAG+GGG | + | chr4.1:17608284-17608303 | MS.gene08404:intron | 40.0% |
| ! | GCCAACTCCTTTTCATCAAA+GGG | + | chr4.1:17608283-17608302 | MS.gene08404:intron | 40.0% |
| ! | TCCTTTTCATCAAAGGGGTT+TGG | + | chr4.1:17608289-17608308 | MS.gene08404:intron | 40.0% |
| ! | TGAAGCCTGTTGGTAGAATA+GGG | + | chr4.1:17608808-17608827 | MS.gene08404:CDS | 40.0% |
| ! | TTGAAGCCTGTTGGTAGAAT+AGG | + | chr4.1:17608807-17608826 | MS.gene08404:CDS | 40.0% |
| !! | GTTGTTGTTGATAGTGGAAG+TGG | - | chr4.1:17607859-17607878 | None:intergenic | 40.0% |
| !! | TTCCTTTTGAAGATTCAGCG+CGG | + | chr4.1:17608611-17608630 | MS.gene08404:intron | 40.0% |
| !!! | GGCACTGATTTTGTTTTTGC+TGG | + | chr4.1:17608568-17608587 | MS.gene08404:intron | 40.0% |
| AAGACTTCCTTGAAGCCTGT+TGG | + | chr4.1:17608798-17608817 | MS.gene08404:CDS | 45.0% | |
| ACTTCCATGGAAGTGGTTGA+TGG | - | chr4.1:17607834-17607853 | None:intergenic | 45.0% | |
| AGTGGAAACTTCCATGGAAG+TGG | - | chr4.1:17607841-17607860 | None:intergenic | 45.0% | |
| AGTGGAAGTGGAAACTTCCA+TGG | - | chr4.1:17607847-17607866 | None:intergenic | 45.0% | |
| CACTATCAACAACAACGCCA+TGG | + | chr4.1:17607863-17607882 | MS.gene08404:CDS | 45.0% | |
| CCCCTTTGATGAAAAGGAGT+TGG | - | chr4.1:17608287-17608306 | None:intergenic | 45.0% | |
| CGAAGTCAATGAGATCGAAG+AGG | + | chr4.1:17607688-17607707 | MS.gene08404:CDS | 45.0% | |
| CGAATTGGCATTTGGTTGCT+TGG | + | chr4.1:17608700-17608719 | MS.gene08404:intron | 45.0% | |
| CTTCCATGGAAGTGGTTGAT+GGG | - | chr4.1:17607833-17607852 | None:intergenic | 45.0% | |
| GAAAGAGTAGGGCAGTGATT+AGG | + | chr4.1:17608148-17608167 | MS.gene08404:intron | 45.0% | |
| GCCAAACCCCTTTGATGAAA+AGG | - | chr4.1:17608293-17608312 | None:intergenic | 45.0% | |
| ! | CATGGCGTTGTTGTTGATAG+TGG | - | chr4.1:17607865-17607884 | None:intergenic | 45.0% |
| ! | CGCCAACTCCTTTTCATCAA+AGG | + | chr4.1:17608282-17608301 | MS.gene08404:intron | 45.0% |
| ! | GCTTTGAATTACCATTGCGG+CGG | - | chr4.1:17608872-17608891 | None:intergenic | 45.0% |
| ! | TCATCAAAGGGGTTTGGCTT+TGG | + | chr4.1:17608295-17608314 | MS.gene08404:intron | 45.0% |
| CAAGATGTGGAGATGGCTGA+TGG | + | chr4.1:17608765-17608784 | MS.gene08404:intron | 50.0% | |
| GGCAGTGATTAGGAAGAGAG+AGG | + | chr4.1:17608158-17608177 | MS.gene08404:intron | 50.0% | |
| GTCCGCGCTGAATCTTCAAA+AGG | - | chr4.1:17608616-17608635 | None:intergenic | 50.0% | |
| TCTTGCTGCTCCTAAGCTTC+AGG | + | chr4.1:17607800-17607819 | MS.gene08404:CDS | 50.0% | |
| TGAGATCGAAGAGGGAGAAG+AGG | + | chr4.1:17607697-17607716 | MS.gene08404:CDS | 50.0% | |
| TTTGCCGAGCCGAAAGAGTA+GGG | + | chr4.1:17608137-17608156 | MS.gene08404:intron | 50.0% | |
| ! | TTTTGCCGAGCCGAAAGAGT+AGG | + | chr4.1:17608136-17608155 | MS.gene08404:intron | 50.0% |
| !! | CATGGAAGTGGTTGATGGGA+CGG | - | chr4.1:17607829-17607848 | None:intergenic | 50.0% |
| ACTGCCCTACTCTTTCGGCT+CGG | - | chr4.1:17608144-17608163 | None:intergenic | 55.0% | |
| AGGAAGAGAGAGGACTCGAG+TGG | + | chr4.1:17608168-17608187 | MS.gene08404:intron | 55.0% | |
| CGTCCCATCAACCACTTCCA+TGG | + | chr4.1:17607827-17607846 | MS.gene08404:CDS | 55.0% | |
| GGAAGAGAGAGGACTCGAGT+GGG | + | chr4.1:17608169-17608188 | MS.gene08404:intron | 55.0% | |
| GGGACGGAAACCTGAAGCTT+AGG | - | chr4.1:17607813-17607832 | None:intergenic | 55.0% | |
| GCTAAAGGCAACCGCCGCAA+TGG | + | chr4.1:17608858-17608877 | MS.gene08404:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17607684 | 17608914 | 17607684 | ID=MS.gene08404 |
| chr4.1 | mRNA | 17607684 | 17608914 | 17607684 | ID=MS.gene08404.t1;Parent=MS.gene08404 |
| chr4.1 | exon | 17607684 | 17607885 | 17607684 | ID=MS.gene08404.t1.exon1;Parent=MS.gene08404.t1 |
| chr4.1 | CDS | 17607684 | 17607885 | 17607684 | ID=cds.MS.gene08404.t1;Parent=MS.gene08404.t1 |
| chr4.1 | exon | 17608769 | 17608914 | 17608769 | ID=MS.gene08404.t1.exon2;Parent=MS.gene08404.t1 |
| chr4.1 | CDS | 17608769 | 17608914 | 17608769 | ID=cds.MS.gene08404.t1;Parent=MS.gene08404.t1 |
| Gene Sequence |
| Protein sequence |
