Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08412.t1 | XP_013457395.1 | 92.7 | 331 | 24 | 0 | 1 | 331 | 1 | 331 | 4.20E-177 | 630.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08412.t1 | Q3TUH1 | 32.6 | 319 | 199 | 6 | 18 | 330 | 24 | 332 | 1.5e-42 | 174.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08412.t1 | G8A1V5 | 92.7 | 331 | 24 | 0 | 1 | 331 | 1 | 331 | 3.0e-177 | 630.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053883 | MS.gene08412 | 0.804536 | 2.06E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene08412 | 0.806012 | 1.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08412.t1 | MTR_4g094760 | 92.749 | 331 | 24 | 0 | 1 | 331 | 1 | 331 | 0.0 | 622 |
| MS.gene08412.t1 | MTR_4g094760 | 92.517 | 294 | 22 | 0 | 38 | 331 | 1 | 294 | 0.0 | 545 |
| MS.gene08412.t1 | MTR_4g094760 | 92.520 | 254 | 19 | 0 | 78 | 331 | 10 | 263 | 2.51e-165 | 461 |
| MS.gene08412.t1 | MTR_4g094760 | 93.562 | 233 | 15 | 0 | 1 | 233 | 1 | 233 | 7.69e-157 | 439 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08412.t1 | AT3G47630 | 65.741 | 324 | 111 | 0 | 6 | 329 | 7 | 330 | 1.97e-156 | 441 |
| MS.gene08412.t1 | AT3G47630 | 65.741 | 324 | 111 | 0 | 6 | 329 | 7 | 330 | 1.97e-156 | 441 |
| MS.gene08412.t1 | AT3G47630 | 65.741 | 324 | 111 | 0 | 6 | 329 | 7 | 330 | 1.97e-156 | 441 |
| MS.gene08412.t1 | AT3G47630 | 65.763 | 295 | 101 | 0 | 35 | 329 | 24 | 318 | 2.41e-138 | 395 |
| MS.gene08412.t1 | AT3G47630 | 66.045 | 268 | 91 | 0 | 62 | 329 | 6 | 273 | 1.13e-123 | 356 |
| MS.gene08412.t1 | AT3G47630 | 62.780 | 223 | 83 | 0 | 107 | 329 | 1 | 223 | 3.71e-92 | 274 |
Find 64 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAGTTTGCTTGCGTTTA+TGG | 0.096576 | 4.1:-17735503 | MS.gene08412:CDS |
| TCAAATTGTTGTTGATAATT+TGG | 0.122916 | 4.1:-17730975 | MS.gene08412:CDS |
| ACCATGATTGATTATATTCT+TGG | 0.194064 | 4.1:-17735364 | MS.gene08412:CDS |
| CTCCTTGCTTCATGCTCAAT+TGG | 0.235122 | 4.1:+17729001 | None:intergenic |
| AATTTGTAATCTCTCATATA+TGG | 0.240141 | 4.1:-17730774 | MS.gene08412:CDS |
| AGACCTGAGATACTTGTATT+TGG | 0.288139 | 4.1:+17730133 | None:intergenic |
| ACCTCAGAATGCCATTGTTT+GGG | 0.293822 | 4.1:+17735331 | None:intergenic |
| TATATGCAGATGTTCAAGTA+TGG | 0.294312 | 4.1:-17734760 | MS.gene08412:intron |
| ACCGCGGTTGCAGATAAAAT+TGG | 0.296872 | 4.1:-17735007 | MS.gene08412:CDS |
| CTCAATTGGCTTCTGATTGA+TGG | 0.298730 | 4.1:+17729015 | None:intergenic |
| ACTTCAAGATGTGCAGTATT+GGG | 0.344897 | 4.1:-17734716 | MS.gene08412:CDS |
| CAGGTGAAGAAGATTGTAAC+TGG | 0.350465 | 4.1:-17730246 | MS.gene08412:CDS |
| TACCTCAGAATGCCATTGTT+TGG | 0.352393 | 4.1:+17735330 | None:intergenic |
| AGTGCAAGGCAGGCCGTATC+TGG | 0.358772 | 4.1:-17728644 | MS.gene08412:CDS |
| GCCTCGTATTCTTCAAGAAA+TGG | 0.368181 | 4.1:+17730192 | None:intergenic |
| TAAACGCAAGCAAACTCAAC+AGG | 0.400684 | 4.1:+17735506 | None:intergenic |
| ATTTGGTGATTGACTGTTGA+TGG | 0.403147 | 4.1:+17730150 | None:intergenic |
| TACTTCAAGATGTGCAGTAT+TGG | 0.416483 | 4.1:-17734717 | MS.gene08412:CDS |
| CTGGCCTACTGGCTGCTGGT+GGG | 0.456512 | 4.1:-17728625 | MS.gene08412:CDS |
| GTATCTGGCCTACTGGCTGC+TGG | 0.457813 | 4.1:-17728629 | MS.gene08412:CDS |
| GACGTGAAATCGAGTCGAAC+TGG | 0.464437 | 4.1:-17728976 | MS.gene08412:intron |
| ATTTGTAATCTCTCATATAT+GGG | 0.464476 | 4.1:-17730773 | MS.gene08412:CDS |
| GCAGGCCGTATCTGGCCTAC+TGG | 0.467270 | 4.1:-17728636 | MS.gene08412:CDS |
| TCAATAAAGCTTGGAAGTCA+TGG | 0.467413 | 4.1:-17728571 | MS.gene08412:CDS |
| TTTCCGTTCCACGTAACAAA+AGG | 0.473223 | 4.1:+17734965 | None:intergenic |
| GCACCTTGGCGGAGAGAGGC+TGG | 0.473894 | 4.1:-17735144 | MS.gene08412:intron |
| TCACCAAATACAAGTATCTC+AGG | 0.479485 | 4.1:-17730136 | MS.gene08412:intron |
| TATGGTTTCAAGTGCAAGGC+AGG | 0.490103 | 4.1:-17728654 | MS.gene08412:CDS |
| TAAACCTCTGTGAATTCTGA+TGG | 0.503955 | 4.1:+17730890 | None:intergenic |
| AGCAGCCAGTAGGCCAGATA+CGG | 0.507076 | 4.1:+17728631 | None:intergenic |
| TGGCCTACTGGCTGCTGGTG+GGG | 0.511323 | 4.1:-17728624 | MS.gene08412:CDS |
| ACCAAGAATATAATCAATCA+TGG | 0.514721 | 4.1:+17735363 | None:intergenic |
| CCAACAAAATCAATAAAGCT+TGG | 0.517401 | 4.1:-17728580 | MS.gene08412:CDS |
| TCTGGCCTACTGGCTGCTGG+TGG | 0.520815 | 4.1:-17728626 | MS.gene08412:CDS |
| ATATGCAGATGTTCAAGTAT+GGG | 0.527202 | 4.1:-17734759 | MS.gene08412:intron |
| AAAGAAGACCTTGTCATGGT+TGG | 0.546678 | 4.1:+17735463 | None:intergenic |
| ACAAGCATCACTATGCATCA+TGG | 0.560182 | 4.1:-17735172 | MS.gene08412:CDS |
| GGGTTATGGTTTCAAGTGCA+AGG | 0.565532 | 4.1:-17728658 | MS.gene08412:CDS |
| TCATGGATGGTGCACCTTGG+CGG | 0.566477 | 4.1:-17735155 | MS.gene08412:CDS |
| TGGTGCACCTTGGCGGAGAG+AGG | 0.568336 | 4.1:-17735148 | MS.gene08412:CDS |
| AAGACCTTGTCATGGTTGGT+TGG | 0.581679 | 4.1:+17735467 | None:intergenic |
| GTACCAGCCTCTCTCCGCCA+AGG | 0.582816 | 4.1:+17735141 | None:intergenic |
| TGGGATAAATTCTACTTGTG+TGG | 0.584350 | 4.1:-17734697 | MS.gene08412:CDS |
| AAACGCAAGCAAACTCAACA+GGG | 0.592632 | 4.1:+17735507 | None:intergenic |
| GAAATGGCTTATACGTCGAG+TGG | 0.597222 | 4.1:+17730208 | None:intergenic |
| AGCCAATTGAGCATGAAGCA+AGG | 0.610932 | 4.1:-17729003 | MS.gene08412:CDS |
| AATTGGCTTCTGATTGATGG+AGG | 0.612804 | 4.1:+17729018 | None:intergenic |
| GTGTTTCTGACCCCAAACAA+TGG | 0.614293 | 4.1:-17735342 | MS.gene08412:CDS |
| GTTGCAGATAAAATTGGCGT+AGG | 0.619343 | 4.1:-17735001 | MS.gene08412:CDS |
| TCATCCAACCAACCATGACA+AGG | 0.620663 | 4.1:-17735471 | MS.gene08412:intron |
| TATGCAGATGTTCAAGTATG+GGG | 0.622561 | 4.1:-17734758 | MS.gene08412:intron |
| CCTCAGAATGCCATTGTTTG+GGG | 0.623734 | 4.1:+17735332 | None:intergenic |
| GCATCACTATGCATCATGGA+TGG | 0.628377 | 4.1:-17735168 | MS.gene08412:CDS |
| TTACCCCACCAGCAGCCAGT+AGG | 0.636171 | 4.1:+17728621 | None:intergenic |
| CCCCAAACAATGGCATTCTG+AGG | 0.637750 | 4.1:-17735332 | MS.gene08412:intron |
| AACAAAAGAAGACCTTGTCA+TGG | 0.645360 | 4.1:+17735459 | None:intergenic |
| TGTGCGCATGTTATTTGCTG+AGG | 0.646332 | 4.1:-17730747 | MS.gene08412:CDS |
| GCATCATGGATGGTGCACCT+TGG | 0.647771 | 4.1:-17735158 | MS.gene08412:CDS |
| GCCATTTCTTGAAGAATACG+AGG | 0.652695 | 4.1:-17730193 | MS.gene08412:CDS |
| AGGTGAAGAAGATTGTAACT+GGG | 0.678071 | 4.1:-17730245 | MS.gene08412:CDS |
| ACGCAAGCAAACTCAACAGG+GGG | 0.689789 | 4.1:+17735509 | None:intergenic |
| AACGCAAGCAAACTCAACAG+GGG | 0.716547 | 4.1:+17735508 | None:intergenic |
| TGTAACATGTAAGATTACCG+CGG | 0.723354 | 4.1:-17735023 | MS.gene08412:intron |
| GCTTCCATCAGAATTCACAG+AGG | 0.776503 | 4.1:-17730894 | MS.gene08412:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17728565 | 17735576 | 17728565 | ID=MS.gene08412 |
| chr4.1 | mRNA | 17728565 | 17735576 | 17728565 | ID=MS.gene08412.t1;Parent=MS.gene08412 |
| chr4.1 | exon | 17735472 | 17735576 | 17735472 | ID=MS.gene08412.t1.exon1;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17735472 | 17735576 | 17735472 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17735333 | 17735389 | 17735333 | ID=MS.gene08412.t1.exon2;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17735333 | 17735389 | 17735333 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17735145 | 17735207 | 17735145 | ID=MS.gene08412.t1.exon3;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17735145 | 17735207 | 17735145 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17734964 | 17735032 | 17734964 | ID=MS.gene08412.t1.exon4;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17734964 | 17735032 | 17734964 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17734681 | 17734773 | 17734681 | ID=MS.gene08412.t1.exon5;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17734681 | 17734773 | 17734681 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17730895 | 17730999 | 17730895 | ID=MS.gene08412.t1.exon6;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17730895 | 17730999 | 17730895 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17730736 | 17730813 | 17730736 | ID=MS.gene08412.t1.exon7;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17730736 | 17730813 | 17730736 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17730137 | 17730265 | 17730137 | ID=MS.gene08412.t1.exon8;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17730137 | 17730265 | 17730137 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17728977 | 17729082 | 17728977 | ID=MS.gene08412.t1.exon9;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17728977 | 17729082 | 17728977 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| chr4.1 | exon | 17728565 | 17728755 | 17728565 | ID=MS.gene08412.t1.exon10;Parent=MS.gene08412.t1 |
| chr4.1 | CDS | 17728565 | 17728755 | 17728565 | ID=cds.MS.gene08412.t1;Parent=MS.gene08412.t1 |
| Gene Sequence |
| Protein sequence |
