AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08452


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08452.t1 MTR_7g109250 88.991 327 31 2 1 322 1 327 0.0 583
MS.gene08452.t1 MTR_7g109250 88.991 327 31 2 1 322 61 387 0.0 582
MS.gene08452.t1 MTR_7g108868 49.438 178 28 3 60 236 11 127 2.38e-47 158
MS.gene08452.t1 MTR_8g058593 49.438 178 28 3 60 236 11 127 2.38e-47 158
MS.gene08452.t1 MTR_8g058607 50.000 180 27 4 60 238 24 141 4.68e-47 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08452.t1 AT1G01350 68.790 314 92 4 13 322 25 336 4.27e-158 446
MS.gene08452.t1 AT5G06420 67.722 316 93 4 11 322 61 371 8.58e-157 444
MS.gene08452.t1 AT5G06420 67.722 316 93 4 11 322 61 371 8.58e-157 444
MS.gene08452.t1 AT4G01023 66.667 57 19 0 255 311 78 134 1.21e-26 105
MS.gene08452.t1 AT4G01023 66.667 57 19 0 255 311 97 153 3.42e-26 104
MS.gene08452.t1 AT4G01023 66.667 57 19 0 255 311 97 153 4.40e-26 104

Find 69 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGAAAGCTAGGTTTCT+TGG 0.236965 8.2:-72551477 None:intergenic
GTGGGTCACACGGTCCTCTT+CGG 0.314266 8.2:+72551741 MS.gene08452:CDS
TTGGTGATTTGCAGGAGATT+TGG 0.316183 8.2:-72551130 None:intergenic
CTCATATTCTTCTTATTAAG+AGG 0.321707 8.2:-72551305 None:intergenic
TGTTCAGTTTGGTGATTTGC+AGG 0.323435 8.2:-72551138 None:intergenic
GATCGAGGTGATTACAAATC+TGG 0.331028 8.2:+72551875 MS.gene08452:CDS
TATACATACCTTGTTCAGTT+TGG 0.352276 8.2:-72551149 None:intergenic
TGGGTCACACGGTCCTCTTC+GGG 0.377787 8.2:+72551742 MS.gene08452:CDS
GGATATATGCAAGGATTATA+AGG 0.382708 8.2:+72551808 MS.gene08452:CDS
TGCAAGGATTATAAGGAAAC+TGG 0.390916 8.2:+72551815 MS.gene08452:CDS
TTATAATCCTTGCATATATC+CGG 0.408034 8.2:-72551806 None:intergenic
GGTTTCTTGGATTCTTCGTT+CGG 0.408554 8.2:-72551464 None:intergenic
TAGCAAAGCAACCGCAACTT+TGG 0.426930 8.2:+72551532 MS.gene08452:CDS
CTTGCTTAAGAGCTCTTTCA+CGG 0.431115 8.2:-72551592 None:intergenic
CTGTGAGCATTGTGCATTGA+AGG 0.436410 8.2:+72552081 MS.gene08452:CDS
GTCTGCAACCAGCCTACGCT+TGG 0.437064 8.2:+72552225 MS.gene08452:CDS
TGCTTGCACTTGGTTGAGAC+AGG 0.443250 8.2:-72552052 None:intergenic
GAAGACTCAAAATGAAAGCT+AGG 0.455285 8.2:-72551485 None:intergenic
GAAACTGGTTACTGTGGGTA+TGG 0.457746 8.2:+72551830 MS.gene08452:CDS
CATTTGTAGAAATCCATTTG+TGG 0.461066 8.2:+72552027 MS.gene08452:CDS
GAAAGCAAGGAGGATGAGAT+TGG 0.475774 8.2:+72551931 MS.gene08452:CDS
CAATAATTACACAGATCATA+AGG 0.476447 8.2:+72551676 MS.gene08452:CDS
GTGGCAGATGGAGAAGGAAT+GGG 0.495492 8.2:+72551898 MS.gene08452:CDS
GCAAACAATTGCCAGTGAAA+AGG 0.499122 8.2:+72551715 MS.gene08452:CDS
AAACTGGTTACTGTGGGTAT+GGG 0.503271 8.2:+72551831 MS.gene08452:CDS
GTTGAGACAGGATCCACAAA+TGG 0.505798 8.2:-72552040 None:intergenic
CTACAAATGAAACACGCAAA+TGG 0.511076 8.2:-72552013 None:intergenic
ATCGAGGTGATTACAAATCT+GGG 0.520829 8.2:+72551876 MS.gene08452:CDS
AGCAAGATTTGATTATCAGC+CGG 0.527438 8.2:+72551787 MS.gene08452:CDS
TTCTTCGTTCGGTTTAGCAT+AGG 0.529758 8.2:-72551453 None:intergenic
TGCAGGTGAGGATGCAGAAG+AGG 0.532110 8.2:+72551955 MS.gene08452:CDS
GGTGGCAGATGGAGAAGGAA+TGG 0.534084 8.2:+72551897 MS.gene08452:CDS
CTCTTAATAAGAAGAATATG+AGG 0.536262 8.2:+72551306 MS.gene08452:CDS
CTTAAGAGCTCTTTCACGGA+TGG 0.538525 8.2:-72551588 None:intergenic
AATTACACAGATCATAAGGC+TGG 0.540860 8.2:+72551680 MS.gene08452:CDS
GCGAAGCACTATTGAGAATG+AGG 0.543921 8.2:+72551331 MS.gene08452:CDS
TTACAAATCTGGGTGGCAGA+TGG 0.553483 8.2:+72551886 MS.gene08452:CDS
TATTAGTATGGAAGATTCTG+AGG 0.556688 8.2:+72551107 None:intergenic
ACAGAAGTAGTGCTTGCACT+TGG 0.557721 8.2:-72552062 None:intergenic
TCATGAGATTCGAAAGAAGA+TGG 0.559186 8.2:+72552263 MS.gene08452:CDS
CAAGCGACAGAGAGTCTCAA+AGG 0.560300 8.2:+72551611 MS.gene08452:CDS
AACTGGTTACTGTGGGTATG+GGG 0.562522 8.2:+72551832 MS.gene08452:CDS
AGGTGAGGATGCAGAAGAGG+AGG 0.575195 8.2:+72551958 MS.gene08452:CDS
ATCTGGGTGGCAGATGGAGA+AGG 0.575589 8.2:+72551892 MS.gene08452:CDS
GGGATGAAGCTGAGAAAGCA+AGG 0.584805 8.2:+72551918 MS.gene08452:CDS
TATAAGGAAACTGGTTACTG+TGG 0.586008 8.2:+72551824 MS.gene08452:CDS
ACAATTGCCAGTGAAAAGGC+TGG 0.588175 8.2:+72551719 MS.gene08452:CDS
GATGAGATTGGCTGCAGGTG+AGG 0.590274 8.2:+72551943 MS.gene08452:CDS
GGCAGAGCTCTTTGAAGAGG+CGG 0.591989 8.2:-72551432 None:intergenic
GCAAATCACCAAACTGAACA+AGG 0.596043 8.2:+72551141 MS.gene08452:CDS
AGAGCTCTTTGAAGAGGCGG+TGG 0.597520 8.2:-72551429 None:intergenic
ATAGGCAGAGCTCTTTGAAG+AGG 0.600392 8.2:-72551435 None:intergenic
GAAAAGGCTGGTGGGTCACA+CGG 0.600903 8.2:+72551731 MS.gene08452:CDS
AGGAGGATGAGATTGGCTGC+AGG 0.603143 8.2:+72551938 MS.gene08452:CDS
ATTGCCAGTGAAAAGGCTGG+TGG 0.607353 8.2:+72551722 MS.gene08452:CDS
AGCACTATTGAGAATGAGGA+TGG 0.610986 8.2:+72551335 MS.gene08452:CDS
GGTGAGGATGCAGAAGAGGA+GGG 0.615274 8.2:+72551959 MS.gene08452:CDS
GATTCGAAAGAAGATGGCTG+AGG 0.618322 8.2:+72552269 MS.gene08452:CDS
ATAAGGAAACTGGTTACTGT+GGG 0.628079 8.2:+72551825 MS.gene08452:CDS
TTATCAGCCGGATATATGCA+AGG 0.629970 8.2:+72551799 MS.gene08452:CDS
TTGCCAGTGAAAAGGCTGGT+GGG 0.630138 8.2:+72551723 MS.gene08452:CDS
AGTCTCAGTCTCCAAAGTTG+CGG 0.639796 8.2:-72551543 None:intergenic
ATGTGAGCTGAAGCCCGAAG+AGG 0.647511 8.2:-72551755 None:intergenic
TGTAAGTTTATGCATGATCG+AGG 0.650874 8.2:+72551860 MS.gene08452:CDS
GAGGATGTAAAATTATACAA+AGG 0.658301 8.2:+72551650 MS.gene08452:CDS
TGAGGATGGCTCGAACAATG+AGG 0.664875 8.2:+72551349 MS.gene08452:CDS
AGGAAAGAGTAATCGTTCCG+AGG 0.677145 8.2:+72551631 MS.gene08452:CDS
ATGAAGCTGAGAAAGCAAGG+AGG 0.730792 8.2:+72551921 MS.gene08452:CDS
GAGGTGATTACAAATCTGGG+TGG 0.758440 8.2:+72551879 MS.gene08452:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTATAATTTTACATCCT+CGG - chr8.2:72551651-72551670 None:intergenic 20.0%
! CAATAATTACACAGATCATA+AGG + chr8.2:72551676-72551695 MS.gene08452:CDS 25.0%
! CTCATATTCTTCTTATTAAG+AGG - chr8.2:72551308-72551327 None:intergenic 25.0%
! CTCTTAATAAGAAGAATATG+AGG + chr8.2:72551306-72551325 MS.gene08452:CDS 25.0%
! GAGGATGTAAAATTATACAA+AGG + chr8.2:72551650-72551669 MS.gene08452:CDS 25.0%
! TTATAATCCTTGCATATATC+CGG - chr8.2:72551809-72551828 None:intergenic 25.0%
!!! TGAATTTGCTTTGAAGTTAA+GGG - chr8.2:72552111-72552130 None:intergenic 25.0%
!!! TTGAATTTGCTTTGAAGTTA+AGG - chr8.2:72552112-72552131 None:intergenic 25.0%
AAAATGAAAGCTAGGTTTCT+TGG - chr8.2:72551480-72551499 None:intergenic 30.0%
AGGAGTTACGCATAATTAAA+AGG - chr8.2:72552164-72552183 None:intergenic 30.0%
AGTTACGCATAATTAAAAGG+GGG - chr8.2:72552161-72552180 None:intergenic 30.0%
CATTTGTAGAAATCCATTTG+TGG + chr8.2:72552027-72552046 MS.gene08452:CDS 30.0%
GAGTTACGCATAATTAAAAG+GGG - chr8.2:72552162-72552181 None:intergenic 30.0%
GGAGTTACGCATAATTAAAA+GGG - chr8.2:72552163-72552182 None:intergenic 30.0%
GGATATATGCAAGGATTATA+AGG + chr8.2:72551808-72551827 MS.gene08452:CDS 30.0%
GTACAAAGAAATAAATGCAG+AGG - chr8.2:72551223-72551242 None:intergenic 30.0%
TATACATACCTTGTTCAGTT+TGG - chr8.2:72551152-72551171 None:intergenic 30.0%
AATTACACAGATCATAAGGC+TGG + chr8.2:72551680-72551699 MS.gene08452:CDS 35.0%
ACATTAAAAATGCCAAGCGT+AGG - chr8.2:72552240-72552259 None:intergenic 35.0%
AGCAAGATTTGATTATCAGC+CGG + chr8.2:72551787-72551806 MS.gene08452:CDS 35.0%
ATAAGGAAACTGGTTACTGT+GGG + chr8.2:72551825-72551844 MS.gene08452:CDS 35.0%
ATCGAGGTGATTACAAATCT+GGG + chr8.2:72551876-72551895 MS.gene08452:CDS 35.0%
ATGATGCTGCAAGTAAAATG+AGG - chr8.2:72552184-72552203 None:intergenic 35.0%
CTACAAATGAAACACGCAAA+TGG - chr8.2:72552016-72552035 None:intergenic 35.0%
GAAGACTCAAAATGAAAGCT+AGG - chr8.2:72551488-72551507 None:intergenic 35.0%
TATAAGGAAACTGGTTACTG+TGG + chr8.2:72551824-72551843 MS.gene08452:CDS 35.0%
TCATGAGATTCGAAAGAAGA+TGG + chr8.2:72552263-72552282 MS.gene08452:CDS 35.0%
TGCAAGGATTATAAGGAAAC+TGG + chr8.2:72551815-72551834 MS.gene08452:CDS 35.0%
TGTAAGTTTATGCATGATCG+AGG + chr8.2:72551860-72551879 MS.gene08452:CDS 35.0%
! TACGCTTGGCATTTTTAATG+TGG + chr8.2:72552239-72552258 MS.gene08452:CDS 35.0%
AAACTGGTTACTGTGGGTAT+GGG + chr8.2:72551831-72551850 MS.gene08452:CDS 40.0%
AGCACTATTGAGAATGAGGA+TGG + chr8.2:72551335-72551354 MS.gene08452:CDS 40.0%
CTTGCTTAAGAGCTCTTTCA+CGG - chr8.2:72551595-72551614 None:intergenic 40.0%
GATCGAGGTGATTACAAATC+TGG + chr8.2:72551875-72551894 MS.gene08452:CDS 40.0%
GCAAACAATTGCCAGTGAAA+AGG + chr8.2:72551715-72551734 MS.gene08452:CDS 40.0%
GCAAATCACCAAACTGAACA+AGG + chr8.2:72551141-72551160 MS.gene08452:CDS 40.0%
GGTTTCTTGGATTCTTCGTT+CGG - chr8.2:72551467-72551486 None:intergenic 40.0%
TTATCAGCCGGATATATGCA+AGG + chr8.2:72551799-72551818 MS.gene08452:CDS 40.0%
TTCTTCGTTCGGTTTAGCAT+AGG - chr8.2:72551456-72551475 None:intergenic 40.0%
! TGTTCAGTTTGGTGATTTGC+AGG - chr8.2:72551141-72551160 None:intergenic 40.0%
! TTGGTGATTTGCAGGAGATT+TGG - chr8.2:72551133-72551152 None:intergenic 40.0%
AACTGGTTACTGTGGGTATG+GGG + chr8.2:72551832-72551851 MS.gene08452:CDS 45.0%
ACAATTGCCAGTGAAAAGGC+TGG + chr8.2:72551719-72551738 MS.gene08452:CDS 45.0%
ACAGAAGTAGTGCTTGCACT+TGG - chr8.2:72552065-72552084 None:intergenic 45.0%
AGGAAAGAGTAATCGTTCCG+AGG + chr8.2:72551631-72551650 MS.gene08452:CDS 45.0%
AGTCTCAGTCTCCAAAGTTG+CGG - chr8.2:72551546-72551565 None:intergenic 45.0%
ATGAAGCTGAGAAAGCAAGG+AGG + chr8.2:72551921-72551940 MS.gene08452:CDS 45.0%
CTTAAGAGCTCTTTCACGGA+TGG - chr8.2:72551591-72551610 None:intergenic 45.0%
GAAACTGGTTACTGTGGGTA+TGG + chr8.2:72551830-72551849 MS.gene08452:CDS 45.0%
GAAAGCAAGGAGGATGAGAT+TGG + chr8.2:72551931-72551950 MS.gene08452:CDS 45.0%
GAGGTGATTACAAATCTGGG+TGG + chr8.2:72551879-72551898 MS.gene08452:CDS 45.0%
GATTCGAAAGAAGATGGCTG+AGG + chr8.2:72552269-72552288 MS.gene08452:CDS 45.0%
GTTGAGACAGGATCCACAAA+TGG - chr8.2:72552043-72552062 None:intergenic 45.0%
TAAAAATGCCAAGCGTAGGC+TGG - chr8.2:72552236-72552255 None:intergenic 45.0%
TAGCAAAGCAACCGCAACTT+TGG + chr8.2:72551532-72551551 MS.gene08452:CDS 45.0%
TTACAAATCTGGGTGGCAGA+TGG + chr8.2:72551886-72551905 MS.gene08452:CDS 45.0%
! ATAGGCAGAGCTCTTTGAAG+AGG - chr8.2:72551438-72551457 None:intergenic 45.0%
! CTGTGAGCATTGTGCATTGA+AGG + chr8.2:72552081-72552100 MS.gene08452:CDS 45.0%
! GCGAAGCACTATTGAGAATG+AGG + chr8.2:72551331-72551350 MS.gene08452:CDS 45.0%
ATTGCCAGTGAAAAGGCTGG+TGG + chr8.2:72551722-72551741 MS.gene08452:CDS 50.0%
CAAGCGACAGAGAGTCTCAA+AGG + chr8.2:72551611-72551630 MS.gene08452:CDS 50.0%
GGGATGAAGCTGAGAAAGCA+AGG + chr8.2:72551918-72551937 MS.gene08452:CDS 50.0%
GTGGCAGATGGAGAAGGAAT+GGG + chr8.2:72551898-72551917 MS.gene08452:CDS 50.0%
TGAGGATGGCTCGAACAATG+AGG + chr8.2:72551349-72551368 MS.gene08452:CDS 50.0%
TGCTTGCACTTGGTTGAGAC+AGG - chr8.2:72552055-72552074 None:intergenic 50.0%
TTGCCAGTGAAAAGGCTGGT+GGG + chr8.2:72551723-72551742 MS.gene08452:CDS 50.0%
AGGTGAGGATGCAGAAGAGG+AGG + chr8.2:72551958-72551977 MS.gene08452:CDS 55.0%
ATCTGGGTGGCAGATGGAGA+AGG + chr8.2:72551892-72551911 MS.gene08452:CDS 55.0%
ATGTGAGCTGAAGCCCGAAG+AGG - chr8.2:72551758-72551777 None:intergenic 55.0%
GGTGAGGATGCAGAAGAGGA+GGG + chr8.2:72551959-72551978 MS.gene08452:CDS 55.0%
GGTGGCAGATGGAGAAGGAA+TGG + chr8.2:72551897-72551916 MS.gene08452:CDS 55.0%
TGCAGGTGAGGATGCAGAAG+AGG + chr8.2:72551955-72551974 MS.gene08452:CDS 55.0%
! AGAGCTCTTTGAAGAGGCGG+TGG - chr8.2:72551432-72551451 None:intergenic 55.0%
! AGGAGGATGAGATTGGCTGC+AGG + chr8.2:72551938-72551957 MS.gene08452:CDS 55.0%
! GAAAAGGCTGGTGGGTCACA+CGG + chr8.2:72551731-72551750 MS.gene08452:CDS 55.0%
! GATGAGATTGGCTGCAGGTG+AGG + chr8.2:72551943-72551962 MS.gene08452:CDS 55.0%
! GGCAGAGCTCTTTGAAGAGG+CGG - chr8.2:72551435-72551454 None:intergenic 55.0%
! TGACCCACCAGCCTTTTCAC+TGG - chr8.2:72551729-72551748 None:intergenic 55.0%
GTCTGCAACCAGCCTACGCT+TGG + chr8.2:72552225-72552244 MS.gene08452:CDS 60.0%
GTGGGTCACACGGTCCTCTT+CGG + chr8.2:72551741-72551760 MS.gene08452:CDS 60.0%
TGGGTCACACGGTCCTCTTC+GGG + chr8.2:72551742-72551761 MS.gene08452:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 72551114 72552299 72551114 ID=MS.gene08452
chr8.2 mRNA 72551114 72552299 72551114 ID=MS.gene08452.t1;Parent=MS.gene08452
chr8.2 exon 72551114 72551162 72551114 ID=MS.gene08452.t1.exon1;Parent=MS.gene08452.t1
chr8.2 CDS 72551114 72551162 72551114 ID=cds.MS.gene08452.t1;Parent=MS.gene08452.t1
chr8.2 exon 72551285 72552102 72551285 ID=MS.gene08452.t1.exon2;Parent=MS.gene08452.t1
chr8.2 CDS 72551285 72552102 72551285 ID=cds.MS.gene08452.t1;Parent=MS.gene08452.t1
chr8.2 exon 72552198 72552299 72552198 ID=MS.gene08452.t1.exon3;Parent=MS.gene08452.t1
chr8.2 CDS 72552198 72552299 72552198 ID=cds.MS.gene08452.t1;Parent=MS.gene08452.t1
Gene Sequence

>MS.gene08452

ATGGAAGATTCTGAGGCCAAATCTCCTGCAAATCACCAAACTGAACAAGGTATGTATAATTAATTTCTTGCATTACTAGACTCACAATTGTTATCTTATATATGCACCTCTGCATTTATTTCTTTGTACTGTCATTAACTTTTCTTTTTACGTGTTTGTTGATCTTCACAGTGTGTAGTTTCTTTAGAAAACCTCTTAATAAGAAGAATATGAGGAAGCGAAGCACTATTGAGAATGAGGATGGCTCGAACAATGAGGAGTCTTTGATGCATGTTCAGAAAAAGATCATGAAAGCTGACAATAACTTATTCTTTTCCACCGCCTCTTCAAAGAGCTCTGCCTATGCTAAACCGAACGAAGAATCCAAGAAACCTAGCTTTCATTTTGAGTCTTCTAAAGAGATTCAAGTTCAACATGATAGCAAAGCAACCGCAACTTTGGAGACTGAGACTGACTTTTCAAGAGATGCTCGTGCCATCCGTGAAAGAGCTCTTAAGCAAGCGACAGAGAGTCTCAAAGGAAAGAGTAATCGTTCCGAGGATGTAAAATTATACAAAGGAATCAATAATTACACAGATCATAAGGCTGGATTTCGTAGAGAGCAAACAATTGCCAGTGAAAAGGCTGGTGGGTCACACGGTCCTCTTCGGGCTTCAGCTCACATAAGAGTTTCAGCAAGATTTGATTATCAGCCGGATATATGCAAGGATTATAAGGAAACTGGTTACTGTGGGTATGGGGATTCGTGTAAGTTTATGCATGATCGAGGTGATTACAAATCTGGGTGGCAGATGGAGAAGGAATGGGATGAAGCTGAGAAAGCAAGGAGGATGAGATTGGCTGCAGGTGAGGATGCAGAAGAGGAGGGTGCTAGTTTGAATAATGATGAAGACTCGTTACCATTTGCGTGTTTCATTTGTAGAAATCCATTTGTGGATCCTGTCTCAACCAAGTGCAAGCACTACTTCTGTGAGCATTGTGCATTGAAGGTACACCCTTAACTTCAAAGCAAATTCAAGTTATCTAGTAATGCTTAATTTTTTTCCCCCTTTTAATTATGCGTAACTCCTCATTTTACTTGCAGCATCATGCAAAGAATAAGAAGTGTTTTGTCTGCAACCAGCCTACGCTTGGCATTTTTAATGTGGCTCATGAGATTCGAAAGAAGATGGCTGAGGATAAATAA

Protein sequence

>MS.gene08452.t1

MEDSEAKSPANHQTEQVCSFFRKPLNKKNMRKRSTIENEDGSNNEESLMHVQKKIMKADNNLFFSTASSKSSAYAKPNEESKKPSFHFESSKEIQVQHDSKATATLETETDFSRDARAIRERALKQATESLKGKSNRSEDVKLYKGINNYTDHKAGFRREQTIASEKAGGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFMHDRGDYKSGWQMEKEWDEAEKARRMRLAAGEDAEEEGASLNNDEDSLPFACFICRNPFVDPVSTKCKHYFCEHCALKHHAKNKKCFVCNQPTLGIFNVAHEIRKKMAEDK