Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08702.t1 | XP_013456005.1 | 96.2 | 79 | 1 | 1 | 1 | 79 | 1 | 77 | 8.40E-31 | 142.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08702.t1 | Q9SKN3 | 64.6 | 79 | 20 | 1 | 1 | 79 | 1 | 71 | 2.4e-18 | 92.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08702.t1 | I3SCI9 | 96.2 | 79 | 1 | 1 | 1 | 79 | 1 | 77 | 6.1e-31 | 142.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056097 | MS.gene08702 | 0.806087 | 9.75E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08702.t1 | MTR_4g058950 | 96.203 | 79 | 1 | 1 | 1 | 79 | 1 | 77 | 5.65e-48 | 150 |
| MS.gene08702.t1 | MTR_7g078440 | 56.579 | 76 | 17 | 1 | 1 | 76 | 1 | 60 | 1.50e-22 | 82.8 |
| MS.gene08702.t1 | MTR_5g033270 | 53.947 | 76 | 12 | 1 | 1 | 76 | 36 | 88 | 2.93e-18 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08702.t1 | AT2G37200 | 54.430 | 79 | 29 | 1 | 1 | 79 | 2 | 73 | 7.20e-21 | 81.3 |
| MS.gene08702.t1 | AT2G37200 | 54.430 | 79 | 29 | 1 | 1 | 79 | 31 | 102 | 1.35e-20 | 81.3 |
| MS.gene08702.t1 | AT2G28370 | 64.557 | 79 | 20 | 1 | 1 | 79 | 1 | 71 | 2.48e-20 | 79.7 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTCTGTCACTGCCTTC+TGG | 0.281533 | 4.2:-44502775 | MS.gene08702:CDS |
| GGCTGCTGCACTATCTGTTA+TGG | 0.379001 | 4.2:-44502813 | MS.gene08702:CDS |
| GTAGTTGGAACTTCTTCAAC+TGG | 0.379154 | 4.2:+44502965 | None:intergenic |
| CAAGGCATGCCTGGTACTAT+TGG | 0.395458 | 4.2:-44502872 | MS.gene08702:CDS |
| GGCATGCCTGGTACTATTGG+TGG | 0.484167 | 4.2:-44502869 | MS.gene08702:CDS |
| ACTGCGAAACACGCAGAGAA+AGG | 0.497789 | 4.2:+44502846 | None:intergenic |
| CCACCACCACCATCAGTAGT+TGG | 0.528851 | 4.2:+44502950 | None:intergenic |
| ACAAAATGTAAATGTTCCTA+TGG | 0.529828 | 4.2:-44502912 | MS.gene08702:CDS |
| TGTTCCTATGGTGAGGATGA+AGG | 0.538780 | 4.2:-44502900 | MS.gene08702:CDS |
| AAGGATATTCAAGGCATGCC+TGG | 0.544386 | 4.2:-44502881 | MS.gene08702:CDS |
| CCAACTACTGATGGTGGTGG+TGG | 0.546534 | 4.2:-44502950 | MS.gene08702:CDS |
| GTGAGGATGAAGGATATTCA+AGG | 0.554642 | 4.2:-44502890 | MS.gene08702:CDS |
| TACCAGAAGGCAGTGACAGA+AGG | 0.577487 | 4.2:+44502773 | None:intergenic |
| GAAGAAGTTCCAACTACTGA+TGG | 0.579056 | 4.2:-44502959 | MS.gene08702:CDS |
| GAAAGGCCACCAATAGTACC+AGG | 0.589357 | 4.2:+44502863 | None:intergenic |
| ATATCCTTCATCCTCACCAT+AGG | 0.600777 | 4.2:+44502896 | None:intergenic |
| AGTGACAGAAGGAAAATCAC+TGG | 0.602419 | 4.2:+44502784 | None:intergenic |
| CAACTGGGTGAATTGAAGCA+TGG | 0.610931 | 4.2:+44502981 | None:intergenic |
| GACAGAAGGAAAATCACTGG+TGG | 0.621848 | 4.2:+44502787 | None:intergenic |
| AAACAAAACGAATTACCAGA+AGG | 0.640862 | 4.2:+44502760 | None:intergenic |
| TAGTTGGAACTTCTTCAACT+GGG | 0.665313 | 4.2:+44502966 | None:intergenic |
| ATGTAAATGTTCCTATGGTG+AGG | 0.675797 | 4.2:-44502907 | MS.gene08702:CDS |
| GTTCCAACTACTGATGGTGG+TGG | 0.688480 | 4.2:-44502953 | MS.gene08702:CDS |
| GAAGTTCCAACTACTGATGG+TGG | 0.707650 | 4.2:-44502956 | MS.gene08702:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAATGTAAATGTTCCTA+TGG | - | chr4.2:44502852-44502871 | MS.gene08702:CDS | 25.0% |
| ATGTAAATGTTCCTATGGTG+AGG | - | chr4.2:44502857-44502876 | MS.gene08702:CDS | 35.0% | |
| TAGTTGGAACTTCTTCAACT+GGG | + | chr4.2:44502801-44502820 | None:intergenic | 35.0% | |
| !!! | TGTTTCGCAGTTTGTTTTTG+CGG | - | chr4.2:44502930-44502949 | MS.gene08702:CDS | 35.0% |
| AGTGACAGAAGGAAAATCAC+TGG | + | chr4.2:44502983-44503002 | None:intergenic | 40.0% | |
| ATATCCTTCATCCTCACCAT+AGG | + | chr4.2:44502871-44502890 | None:intergenic | 40.0% | |
| GAAGAAGTTCCAACTACTGA+TGG | - | chr4.2:44502805-44502824 | MS.gene08702:CDS | 40.0% | |
| GTAGTTGGAACTTCTTCAAC+TGG | + | chr4.2:44502802-44502821 | None:intergenic | 40.0% | |
| GTGAGGATGAAGGATATTCA+AGG | - | chr4.2:44502874-44502893 | MS.gene08702:CDS | 40.0% | |
| AAGGATATTCAAGGCATGCC+TGG | - | chr4.2:44502883-44502902 | MS.gene08702:CDS | 45.0% | |
| CAACTGGGTGAATTGAAGCA+TGG | + | chr4.2:44502786-44502805 | None:intergenic | 45.0% | |
| GAAGTTCCAACTACTGATGG+TGG | - | chr4.2:44502808-44502827 | MS.gene08702:CDS | 45.0% | |
| GACAGAAGGAAAATCACTGG+TGG | + | chr4.2:44502980-44502999 | None:intergenic | 45.0% | |
| TGTTCCTATGGTGAGGATGA+AGG | - | chr4.2:44502864-44502883 | MS.gene08702:CDS | 45.0% | |
| ACTGCGAAACACGCAGAGAA+AGG | + | chr4.2:44502921-44502940 | None:intergenic | 50.0% | |
| CAAGGCATGCCTGGTACTAT+TGG | - | chr4.2:44502892-44502911 | MS.gene08702:CDS | 50.0% | |
| GAAAGGCCACCAATAGTACC+AGG | + | chr4.2:44502904-44502923 | None:intergenic | 50.0% | |
| GGCTGCTGCACTATCTGTTA+TGG | - | chr4.2:44502951-44502970 | MS.gene08702:CDS | 50.0% | |
| TACCAGAAGGCAGTGACAGA+AGG | + | chr4.2:44502994-44503013 | None:intergenic | 50.0% | |
| TTCCTTCTGTCACTGCCTTC+TGG | - | chr4.2:44502989-44503008 | MS.gene08702:CDS | 50.0% | |
| !! | GTTCCAACTACTGATGGTGG+TGG | - | chr4.2:44502811-44502830 | MS.gene08702:CDS | 50.0% |
| CCACCACCACCATCAGTAGT+TGG | + | chr4.2:44502817-44502836 | None:intergenic | 55.0% | |
| GGCATGCCTGGTACTATTGG+TGG | - | chr4.2:44502895-44502914 | MS.gene08702:CDS | 55.0% | |
| !! | CCAACTACTGATGGTGGTGG+TGG | - | chr4.2:44502814-44502833 | MS.gene08702:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 44502772 | 44503014 | 44502772 | ID=MS.gene08702 |
| chr4.2 | mRNA | 44502772 | 44503014 | 44502772 | ID=MS.gene08702.t1;Parent=MS.gene08702 |
| chr4.2 | exon | 44502772 | 44503014 | 44502772 | ID=MS.gene08702.t1.exon1;Parent=MS.gene08702.t1 |
| chr4.2 | CDS | 44502772 | 44503014 | 44502772 | ID=cds.MS.gene08702.t1;Parent=MS.gene08702.t1 |
| Gene Sequence |
| Protein sequence |
