Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08885.t1 | XP_013455967.1 | 89.7 | 136 | 12 | 1 | 1 | 134 | 1 | 136 | 2.70E-61 | 244.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08885.t1 | P0DKL1 | 42.1 | 76 | 41 | 2 | 16 | 91 | 18 | 90 | 3.5e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08885.t1 | A0A072ULQ3 | 89.7 | 136 | 12 | 1 | 1 | 134 | 1 | 136 | 1.9e-61 | 244.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08885 | MS.gene22418 | PPI |
| MS.gene08885 | MS.gene20078 | PPI |
| MS.gene08885 | MS.gene007530 | PPI |
| MS.gene08885 | MS.gene007417 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08885.t1 | MTR_4g057800 | 89.706 | 136 | 12 | 1 | 1 | 134 | 1 | 136 | 6.84e-87 | 249 |
| MS.gene08885.t1 | MTR_4g057810 | 83.333 | 108 | 18 | 0 | 1 | 108 | 1 | 108 | 1.61e-64 | 192 |
| MS.gene08885.t1 | MTR_2g095430 | 56.190 | 105 | 40 | 2 | 5 | 107 | 16 | 116 | 1.69e-36 | 122 |
| MS.gene08885.t1 | MTR_1g063330 | 46.667 | 75 | 40 | 0 | 26 | 100 | 20 | 94 | 1.52e-20 | 80.5 |
| MS.gene08885.t1 | MTR_1g063340 | 45.333 | 75 | 41 | 0 | 26 | 100 | 20 | 94 | 9.20e-20 | 78.6 |
| MS.gene08885.t1 | MTR_1g063300 | 44.318 | 88 | 49 | 0 | 13 | 100 | 14 | 101 | 1.01e-19 | 78.6 |
| MS.gene08885.t1 | MTR_1g063290 | 42.045 | 88 | 51 | 0 | 13 | 100 | 14 | 101 | 3.08e-19 | 77.4 |
| MS.gene08885.t1 | MTR_1g063310 | 41.772 | 79 | 46 | 0 | 33 | 111 | 21 | 99 | 5.10e-18 | 73.9 |
| MS.gene08885.t1 | MTR_1g063280 | 50.746 | 67 | 33 | 0 | 34 | 100 | 38 | 104 | 2.90e-17 | 72.4 |
| MS.gene08885.t1 | MTR_1g063260 | 50.746 | 67 | 33 | 0 | 34 | 100 | 37 | 103 | 5.62e-17 | 71.6 |
| MS.gene08885.t1 | MTR_7g101920 | 43.836 | 73 | 41 | 0 | 33 | 105 | 30 | 102 | 1.83e-16 | 70.5 |
| MS.gene08885.t1 | MTR_7g101890 | 44.444 | 63 | 35 | 0 | 38 | 100 | 52 | 114 | 1.18e-14 | 65.9 |
| MS.gene08885.t1 | MTR_8g076040 | 43.243 | 74 | 40 | 1 | 26 | 99 | 29 | 100 | 1.83e-14 | 65.1 |
| MS.gene08885.t1 | MTR_5g021820 | 45.455 | 66 | 34 | 1 | 26 | 91 | 28 | 91 | 2.90e-14 | 64.7 |
| MS.gene08885.t1 | MTR_3g117610 | 54.545 | 55 | 23 | 1 | 37 | 91 | 44 | 96 | 3.30e-13 | 62.0 |
| MS.gene08885.t1 | MTR_4g072190 | 43.902 | 82 | 38 | 3 | 11 | 91 | 13 | 87 | 3.94e-13 | 61.6 |
| MS.gene08885.t1 | MTR_3g113310 | 46.032 | 63 | 32 | 1 | 37 | 99 | 41 | 101 | 2.08e-12 | 59.7 |
| MS.gene08885.t1 | MTR_4g072910 | 36.364 | 88 | 46 | 3 | 6 | 92 | 10 | 88 | 3.30e-12 | 59.3 |
| MS.gene08885.t1 | MTR_3g117640 | 51.786 | 56 | 25 | 2 | 37 | 91 | 33 | 87 | 6.97e-11 | 55.8 |
| MS.gene08885.t1 | MTR_4g124750 | 36.458 | 96 | 58 | 2 | 5 | 100 | 37 | 129 | 9.35e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08885.t1 | AT2G28085 | 46.296 | 108 | 51 | 3 | 4 | 104 | 16 | 123 | 1.53e-24 | 91.7 |
| MS.gene08885.t1 | AT3G09870 | 46.269 | 67 | 36 | 0 | 34 | 100 | 41 | 107 | 1.19e-15 | 68.6 |
| MS.gene08885.t1 | AT2G21220 | 56.364 | 55 | 22 | 1 | 37 | 91 | 39 | 91 | 1.20e-13 | 63.2 |
| MS.gene08885.t1 | AT4G34760 | 45.070 | 71 | 37 | 1 | 29 | 99 | 34 | 102 | 2.14e-12 | 59.7 |
| MS.gene08885.t1 | AT4G34780 | 46.667 | 60 | 30 | 2 | 37 | 94 | 27 | 86 | 4.28e-12 | 58.9 |
| MS.gene08885.t1 | AT4G38860 | 48.438 | 64 | 30 | 2 | 29 | 91 | 31 | 92 | 5.70e-12 | 58.5 |
| MS.gene08885.t1 | AT5G53590 | 37.662 | 77 | 43 | 2 | 37 | 110 | 45 | 119 | 7.50e-12 | 59.3 |
| MS.gene08885.t1 | AT3G61900 | 43.548 | 62 | 34 | 1 | 31 | 91 | 23 | 84 | 9.74e-12 | 58.9 |
| MS.gene08885.t1 | AT1G19830 | 43.478 | 69 | 37 | 1 | 37 | 105 | 47 | 113 | 1.70e-11 | 57.8 |
| MS.gene08885.t1 | AT4G34800 | 43.421 | 76 | 41 | 2 | 27 | 100 | 10 | 85 | 1.77e-11 | 57.0 |
| MS.gene08885.t1 | AT1G75580 | 50.909 | 55 | 25 | 1 | 37 | 91 | 43 | 95 | 2.40e-11 | 57.0 |
| MS.gene08885.t1 | AT5G66260 | 44.444 | 63 | 33 | 1 | 38 | 100 | 34 | 94 | 2.55e-11 | 57.0 |
| MS.gene08885.t1 | AT2G37030 | 41.270 | 63 | 35 | 1 | 30 | 92 | 41 | 101 | 3.15e-11 | 57.4 |
| MS.gene08885.t1 | AT4G12410 | 39.189 | 74 | 41 | 2 | 19 | 91 | 60 | 130 | 6.26e-11 | 57.0 |
Find 0 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGAAATGATTATAAATCA+AGG | - | 33208:4769-4788 | MS.gene08885:CDS | 20.0% |
| !! | TAATAAACTTCAGATGTTAT+TGG | - | 33208:4974-4993 | MS.gene08885:CDS | 20.0% |
| !!! | AATAACATCTGAAGTTTATT+AGG | + | 33208:4975-4994 | MS.gene08885:intergenic | 20.0% |
| !!! | ATAACATCTGAAGTTTATTA+GGG | + | 33208:4974-4993 | MS.gene08885:intergenic | 20.0% |
| ! | AATCATTTCTTCCCATTAAT+AGG | + | 33208:4761-4780 | MS.gene08885:intergenic | 25.0% |
| ! | ATCATTTCTTCCCATTAATA+GGG | + | 33208:4760-4779 | MS.gene08885:intergenic | 25.0% |
| ! | TGATTATAAATCAAGGTATG+TGG | - | 33208:4776-4795 | MS.gene08885:CDS | 25.0% |
| !!! | TTTAACTTGAAATATCCACT+CGG | + | 33208:5068-5087 | MS.gene08885:intergenic | 25.0% |
| AAGTATGGATTTAATGGAGA+TGG | - | 33208:4927-4946 | MS.gene08885:CDS | 30.0% | |
| CCAATAGACTCACAAATATT+GGG | + | 33208:4893-4912 | MS.gene08885:intergenic | 30.0% | |
| CTCCAAAAAACCCTATTAAT+GGG | - | 33208:4747-4766 | MS.gene08885:CDS | 30.0% | |
| TCCAATAGACTCACAAATAT+TGG | + | 33208:4894-4913 | MS.gene08885:intergenic | 30.0% | |
| ! | GCTGAAAAGTATGGATTTAA+TGG | - | 33208:4921-4940 | MS.gene08885:CDS | 30.0% |
| !!! | TTCCCATTAATAGGGTTTTT+TGG | + | 33208:4752-4771 | MS.gene08885:intergenic | 30.0% |
| CCCAATATTTGTGAGTCTAT+TGG | - | 33208:4890-4909 | MS.gene08885:CDS | 35.0% | |
| CCTCCAAAAAACCCTATTAA+TGG | - | 33208:4746-4765 | MS.gene08885:CDS | 35.0% | |
| TAAATCAAGGTATGTGGCAA+AGG | - | 33208:4782-4801 | MS.gene08885:CDS | 35.0% | |
| TGAAGTTTATTAGGGCTACA+AGG | + | 33208:4966-4985 | MS.gene08885:intergenic | 35.0% | |
| ! | GAAAAAGGGACATTTTGTTG+TGG | - | 33208:4809-4828 | MS.gene08885:CDS | 35.0% |
| !! | ACATTTTGTTGTGGTTGCTA+AGG | - | 33208:4818-4837 | MS.gene08885:CDS | 35.0% |
| !! | GGGTTTGTTAAACAACTCAA+TGG | + | 33208:4873-4892 | MS.gene08885:intergenic | 35.0% |
| !!! | CCATTAATAGGGTTTTTTGG+AGG | + | 33208:4749-4768 | MS.gene08885:intergenic | 35.0% |
| GAACAAGCAGCTGAAAAGTA+TGG | - | 33208:4912-4931 | MS.gene08885:CDS | 40.0% | |
| GTGGCAAAGGATGTGAAAAA+GGG | - | 33208:4795-4814 | MS.gene08885:CDS | 40.0% | |
| TGTGGCAAAGGATGTGAAAA+AGG | - | 33208:4794-4813 | MS.gene08885:CDS | 40.0% | |
| AATATCCACTCGGTCGTCTT+TGG | + | 33208:5058-5077 | MS.gene08885:intergenic | 45.0% | |
| GTTATTGGTGCAGCAATTGC+AGG | - | 33208:4989-5008 | MS.gene08885:CDS | 45.0% | |
| GTGCAGCAATTGCAGGAAGA+AGG | - | 33208:4996-5015 | MS.gene08885:CDS | 50.0% | |
| TGTTACCAAAGACGACCGAG+TGG | - | 33208:5050-5069 | MS.gene08885:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33208 | gene | 4696 | 5109 | 4696 | ID=MS.gene08885 |
| 33208 | mRNA | 4696 | 5109 | 4696 | ID=MS.gene08885.t1;Parent=MS.gene08885 |
| 33208 | exon | 4696 | 5109 | 4696 | ID=MS.gene08885.t1.exon1;Parent=MS.gene08885.t1 |
| 33208 | CDS | 4696 | 5109 | 4696 | ID=cds.MS.gene08885.t1;Parent=MS.gene08885.t1 |
| Gene Sequence |
| Protein sequence |
