Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08886.t1 | XP_013455967.1 | 84.4 | 135 | 19 | 1 | 1 | 133 | 1 | 135 | 1.20E-56 | 229.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08886.t1 | Q9ZUZ3 | 32.9 | 82 | 54 | 1 | 19 | 99 | 5 | 86 | 2.3e-08 | 60.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08886.t1 | A0A072ULQ3 | 84.4 | 135 | 19 | 1 | 1 | 133 | 1 | 135 | 8.5e-57 | 229.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08886 | MS.gene22418 | PPI |
| MS.gene007417 | MS.gene08886 | PPI |
| MS.gene08886 | MS.gene20078 | PPI |
| MS.gene08886 | MS.gene007530 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08886.t1 | MTR_4g057800 | 83.824 | 136 | 20 | 1 | 1 | 134 | 1 | 136 | 3.56e-81 | 235 |
| MS.gene08886.t1 | MTR_4g057810 | 86.111 | 108 | 15 | 0 | 1 | 108 | 1 | 108 | 1.91e-66 | 197 |
| MS.gene08886.t1 | MTR_2g095430 | 56.190 | 105 | 40 | 2 | 5 | 107 | 16 | 116 | 1.81e-36 | 122 |
| MS.gene08886.t1 | MTR_1g063300 | 46.591 | 88 | 47 | 0 | 13 | 100 | 14 | 101 | 7.02e-21 | 81.6 |
| MS.gene08886.t1 | MTR_1g063290 | 51.389 | 72 | 35 | 0 | 29 | 100 | 30 | 101 | 1.07e-20 | 81.3 |
| MS.gene08886.t1 | MTR_1g063280 | 54.545 | 66 | 30 | 0 | 35 | 100 | 39 | 104 | 3.73e-19 | 77.4 |
| MS.gene08886.t1 | MTR_1g063330 | 46.667 | 75 | 40 | 0 | 26 | 100 | 20 | 94 | 4.15e-19 | 76.6 |
| MS.gene08886.t1 | MTR_1g063340 | 46.667 | 75 | 40 | 0 | 26 | 100 | 20 | 94 | 4.47e-19 | 76.6 |
| MS.gene08886.t1 | MTR_1g063260 | 54.545 | 66 | 30 | 0 | 35 | 100 | 38 | 103 | 7.14e-19 | 76.6 |
| MS.gene08886.t1 | MTR_1g063310 | 45.882 | 85 | 43 | 2 | 16 | 100 | 7 | 88 | 1.00e-18 | 75.9 |
| MS.gene08886.t1 | MTR_7g101920 | 43.836 | 73 | 41 | 0 | 33 | 105 | 30 | 102 | 6.87e-17 | 71.6 |
| MS.gene08886.t1 | MTR_7g101890 | 46.377 | 69 | 37 | 0 | 38 | 106 | 52 | 120 | 1.16e-16 | 71.2 |
| MS.gene08886.t1 | MTR_7g118010 | 34.722 | 72 | 47 | 0 | 28 | 99 | 13 | 84 | 3.53e-12 | 59.3 |
| MS.gene08886.t1 | MTR_3g092220 | 30.137 | 73 | 51 | 0 | 27 | 99 | 22 | 94 | 6.24e-12 | 58.9 |
| MS.gene08886.t1 | MTR_3g117610 | 48.214 | 56 | 27 | 1 | 37 | 92 | 44 | 97 | 1.11e-11 | 58.2 |
| MS.gene08886.t1 | MTR_4g072910 | 35.632 | 87 | 46 | 3 | 6 | 91 | 10 | 87 | 1.47e-11 | 57.4 |
| MS.gene08886.t1 | MTR_4g072190 | 39.759 | 83 | 42 | 3 | 11 | 92 | 13 | 88 | 1.56e-11 | 57.4 |
| MS.gene08886.t1 | MTR_8g076040 | 40.909 | 66 | 37 | 1 | 26 | 91 | 29 | 92 | 1.74e-11 | 57.4 |
| MS.gene08886.t1 | MTR_5g021820 | 39.394 | 66 | 38 | 1 | 26 | 91 | 28 | 91 | 2.19e-11 | 57.0 |
| MS.gene08886.t1 | MTR_4g072160 | 38.144 | 97 | 54 | 3 | 4 | 100 | 7 | 97 | 2.29e-11 | 57.8 |
| MS.gene08886.t1 | MTR_0388s0020 | 40.625 | 64 | 37 | 1 | 37 | 99 | 74 | 137 | 7.43e-11 | 56.6 |
| MS.gene08886.t1 | MTR_4g072570 | 34.524 | 84 | 52 | 2 | 17 | 99 | 4 | 85 | 8.78e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08886.t1 | AT2G28085 | 46.296 | 108 | 51 | 3 | 4 | 104 | 16 | 123 | 1.10e-23 | 89.4 |
| MS.gene08886.t1 | AT3G09870 | 50.000 | 64 | 32 | 0 | 37 | 100 | 44 | 107 | 5.16e-16 | 69.3 |
| MS.gene08886.t1 | AT5G53590 | 37.662 | 77 | 43 | 2 | 37 | 110 | 45 | 119 | 3.56e-12 | 60.1 |
| MS.gene08886.t1 | AT2G21220 | 50.909 | 55 | 25 | 1 | 37 | 91 | 39 | 91 | 4.77e-12 | 58.9 |
| MS.gene08886.t1 | AT3G61900 | 38.710 | 62 | 37 | 1 | 31 | 91 | 23 | 84 | 1.40e-11 | 58.5 |
| MS.gene08886.t1 | AT4G34800 | 44.118 | 68 | 36 | 2 | 27 | 92 | 10 | 77 | 3.46e-11 | 56.2 |
| MS.gene08886.t1 | AT1G19830 | 33.654 | 104 | 66 | 2 | 2 | 105 | 13 | 113 | 4.74e-11 | 56.6 |
| MS.gene08886.t1 | AT4G34790 | 41.429 | 70 | 39 | 2 | 38 | 105 | 39 | 108 | 6.84e-11 | 55.8 |
| MS.gene08886.t1 | AT2G46690 | 32.927 | 82 | 54 | 1 | 19 | 99 | 5 | 86 | 7.49e-11 | 56.2 |
| MS.gene08886.t1 | AT4G12410 | 32.927 | 82 | 51 | 2 | 19 | 99 | 60 | 138 | 7.76e-11 | 57.0 |
| MS.gene08886.t1 | AT4G34780 | 43.860 | 57 | 30 | 2 | 37 | 91 | 27 | 83 | 8.63e-11 | 55.8 |
Find 0 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATGGATTTAATGGAAA+TGG | - | 33208:12080-12099 | MS.gene08886:CDS | 20.0% |
| !! | TTTAAATTGAAATATCCACT+AGG | + | 33208:12221-12240 | MS.gene08886:intergenic | 20.0% |
| !!! | AATATCATTTGAAGTTCATT+AGG | + | 33208:12128-12147 | MS.gene08886:intergenic | 20.0% |
| ! | AACATTTCTTCCCATTAATA+GGG | + | 33208:11913-11932 | MS.gene08886:intergenic | 25.0% |
| ! | GAAGAAATGTTCATAAATCA+AGG | - | 33208:11922-11941 | MS.gene08886:CDS | 25.0% |
| ! | GCTGAAAAATATGGATTTAA+TGG | - | 33208:12074-12093 | MS.gene08886:CDS | 25.0% |
| ! | TCTAATAGACTCACAAATAT+TGG | + | 33208:12047-12066 | MS.gene08886:intergenic | 25.0% |
| CTCCAAAAAACCCTATTAAT+GGG | - | 33208:11900-11919 | MS.gene08886:CDS | 30.0% | |
| GAACATTTCTTCCCATTAAT+AGG | + | 33208:11914-11933 | MS.gene08886:intergenic | 30.0% | |
| TGTTCATAAATCAAGGTATG+CGG | - | 33208:11929-11948 | MS.gene08886:CDS | 30.0% | |
| !! | ACATTTTGTTGTGATTGCTA+AGG | - | 33208:11971-11990 | MS.gene08886:CDS | 30.0% |
| !! | GGATTTGTTAAACAACTCAA+TGG | + | 33208:12026-12045 | MS.gene08886:intergenic | 30.0% |
| !!! | TTCCCATTAATAGGGTTTTT+TGG | + | 33208:11905-11924 | MS.gene08886:intergenic | 30.0% |
| GAACAAGCAGCTGAAAAATA+TGG | - | 33208:12065-12084 | MS.gene08886:CDS | 35.0% | |
| GCTCCAAAAAACCCTATTAA+TGG | - | 33208:11899-11918 | MS.gene08886:CDS | 35.0% | |
| AATATCCACTAGGTCGTCTT+TGG | + | 33208:12211-12230 | MS.gene08885:intergenic | 40.0% | |
| GATATTAGTGCAGCAATTGC+AGG | - | 33208:12142-12161 | MS.gene08886:CDS | 40.0% | |
| TGAAGTTCATTAGGTCGACA+AGG | + | 33208:12119-12138 | MS.gene08886:intergenic | 40.0% | |
| GCGGCACAAGATGTGAAAAA+GGG | - | 33208:11948-11967 | MS.gene08886:CDS | 45.0% | |
| TGCGGCACAAGATGTGAAAA+AGG | - | 33208:11947-11966 | MS.gene08886:CDS | 45.0% | |
| TGTTACCAAAGACGACCTAG+TGG | - | 33208:12203-12222 | MS.gene08886:CDS | 45.0% | |
| GTGCAGCAATTGCAGGATGA+AGG | - | 33208:12149-12168 | MS.gene08886:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33208 | gene | 11849 | 12265 | 11849 | ID=MS.gene08886 |
| 33208 | mRNA | 11849 | 12265 | 11849 | ID=MS.gene08886.t1;Parent=MS.gene08886 |
| 33208 | exon | 11849 | 12265 | 11849 | ID=MS.gene08886.t1.exon1;Parent=MS.gene08886.t1 |
| 33208 | CDS | 11849 | 12265 | 11849 | ID=cds.MS.gene08886.t1;Parent=MS.gene08886.t1 |
| Gene Sequence |
| Protein sequence |
