AlfalfaGEDB | MS.gene09341

M. sativa cultivar XinJiangDaYe / MS.gene09341

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09341.t1	XP_013451947.1	80.5	154	6	1	2	131	208	361	4.40E-61	243.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09341.t1	Q9SZ83	54.5	145	42	1	9	129	215	359	2.6e-38	159.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09341.t1	A0A072UJH2	80.5	154	6	1	2	131	208	361	3.2e-61	243.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055133	MS.gene09341	0.800357	1.50E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09341.t1	MTR_6g038640	80.519	154	6	1	2	131	208	361	6.39e-83	247
MS.gene09341.t1	MTR_6g038650	76.667	150	12	2	3	131	209	356	9.66e-73	221
MS.gene09341.t1	MTR_1g097250	64.935	154	30	1	2	131	207	360	4.31e-69	212
MS.gene09341.t1	MTR_6g038350	48.993	149	52	1	6	130	214	362	2.88e-43	145
MS.gene09341.t1	MTR_6g038370	48.993	149	52	1	6	130	214	362	2.88e-43	145
MS.gene09341.t1	MTR_6g038390	47.651	149	54	1	6	130	214	362	4.47e-43	145
MS.gene09341.t1	MTR_6g066230	42.308	156	61	3	2	129	208	362	1.93e-32	117
MS.gene09341.t1	MTR_3g064900	37.931	145	64	2	11	129	219	363	7.29e-26	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09341.t1	AT4G09670	54.483	145	42	1	9	129	215	359	3.04e-49	161
MS.gene09341.t1	AT1G34200	47.482	139	60	2	2	127	209	347	7.87e-36	126
MS.gene09341.t1	AT1G66130	36.000	150	63	3	11	129	217	364	1.16e-22	91.3

Find 26 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTATCACTCCAGCTTTATTA+AGG	0.288328	6.2:+33253765	None:intergenic
TTGTGGTGCTTCTTTATATT+GGG	0.306468	6.2:-33253738	MS.gene09341:CDS
CTTGTGGTGCTTCTTTATAT+TGG	0.315248	6.2:-33253739	MS.gene09341:CDS
CAGCAGTTATATCCATTGTT+AGG	0.316283	6.2:+33253672	None:intergenic
TAGGAAAACACAACTGATAT+TGG	0.320047	6.2:-33253402	MS.gene09341:CDS
TTGATGATCTTGTCACTAGA+TGG	0.323932	6.2:-33253559	MS.gene09341:CDS
TGGCCATTTCTTCTCTGGTT+TGG	0.348763	6.2:+33253433	None:intergenic
ATCGTTGGCCATTTCTTCTC+TGG	0.367938	6.2:+33253428	None:intergenic
CGTGAAGGCTTCGATTGATA+AGG	0.397262	6.2:-33253375	MS.gene09341:CDS
ATAACTGCTGTTGGGACAAA+AGG	0.403721	6.2:-33253659	MS.gene09341:CDS
ACGCCAAACCAGAGAAGAAA+TGG	0.420502	6.2:-33253436	MS.gene09341:CDS
TGATGATCTTGTCACTAGAT+GGG	0.427776	6.2:-33253558	MS.gene09341:CDS
GTGAAGGCTTCGATTGATAA+GGG	0.430595	6.2:-33253374	MS.gene09341:CDS
GCTTCTTTATATTGGGAAGA+TGG	0.456272	6.2:-33253731	MS.gene09341:CDS
CTTATCAATCGAAGCCTTCA+CGG	0.476737	6.2:+33253376	None:intergenic
AGAAATGGCCAACGATTAGT+AGG	0.477786	6.2:-33253421	MS.gene09341:CDS
GATGATCTTGTCACTAGATG+GGG	0.507725	6.2:-33253557	MS.gene09341:CDS
ACAATGGATATAACTGCTGT+TGG	0.509647	6.2:-33253668	MS.gene09341:CDS
CAATGGATATAACTGCTGTT+GGG	0.519696	6.2:-33253667	MS.gene09341:CDS
ACTCCAGCTTTATTAAGGAC+TGG	0.534007	6.2:+33253770	None:intergenic
ACTCATTCCTGAATCTGTAC+AGG	0.557692	6.2:+33253344	None:intergenic
TTTCTTATCTAACCTAACAA+TGG	0.559985	6.2:-33253684	MS.gene09341:CDS
GAGCCAGTCCTTAATAAAGC+TGG	0.570962	6.2:-33253773	MS.gene09341:CDS
ATTAAGGACTGGCTCGCGCG+TGG	0.571573	6.2:+33253781	None:intergenic
ACTGATATTGGATGCCGTGA+AGG	0.586470	6.2:-33253390	MS.gene09341:CDS
GCTGGAGTGATAGTATCTTG+TGG	0.737909	6.2:-33253755	MS.gene09341:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTCTTATCTAACCTAACAA+TGG	-	chr6.2:33253453-33253472	MS.gene09341:CDS	25.0%
!!	ATGCTTTCTTTTCTTCATAA+GGG	+	chr6.2:33253531-33253550	None:intergenic	25.0%
!!	TTTTCTATTACATGCTTACT+CGG	+	chr6.2:33253613-33253632	None:intergenic	25.0%
	TAGGAAAACACAACTGATAT+TGG	-	chr6.2:33253735-33253754	MS.gene09341:CDS	30.0%
	TTGTGGTGCTTCTTTATATT+GGG	-	chr6.2:33253399-33253418	MS.gene09341:CDS	30.0%
!	GATGCTTTCTTTTCTTCATA+AGG	+	chr6.2:33253532-33253551	None:intergenic	30.0%
	AATAGAAAACGATCTACCTC+AGG	-	chr6.2:33253624-33253643	MS.gene09341:CDS	35.0%
	ACAATGGATATAACTGCTGT+TGG	-	chr6.2:33253469-33253488	MS.gene09341:intron	35.0%
	CAATGGATATAACTGCTGTT+GGG	-	chr6.2:33253470-33253489	MS.gene09341:intron	35.0%
	CAGCAGTTATATCCATTGTT+AGG	+	chr6.2:33253468-33253487	None:intergenic	35.0%
	CTTGTGGTGCTTCTTTATAT+TGG	-	chr6.2:33253398-33253417	MS.gene09341:CDS	35.0%
	GCTTCTTTATATTGGGAAGA+TGG	-	chr6.2:33253406-33253425	MS.gene09341:CDS	35.0%
	TGATGATCTTGTCACTAGAT+GGG	-	chr6.2:33253579-33253598	MS.gene09341:CDS	35.0%
!	AACCTAATTTTCTCGTTTGG+TGG	-	chr6.2:33253660-33253679	MS.gene09341:CDS	35.0%
!	GGTAACCTAATTTTCTCGTT+TGG	-	chr6.2:33253657-33253676	MS.gene09341:CDS	35.0%
!	TGTGTTTTCCTACTAATCGT+TGG	+	chr6.2:33253727-33253746	None:intergenic	35.0%
!	TTGATGATCTTGTCACTAGA+TGG	-	chr6.2:33253578-33253597	MS.gene09341:CDS	35.0%
!!	CTATCACTCCAGCTTTATTA+AGG	+	chr6.2:33253375-33253394	None:intergenic	35.0%
	AGAAATGGCCAACGATTAGT+AGG	-	chr6.2:33253716-33253735	MS.gene09341:CDS	40.0%
	ATAACTGCTGTTGGGACAAA+AGG	-	chr6.2:33253478-33253497	MS.gene09341:intron	40.0%
	CTTATCAATCGAAGCCTTCA+CGG	+	chr6.2:33253764-33253783	None:intergenic	40.0%
	GATGATCTTGTCACTAGATG+GGG	-	chr6.2:33253580-33253599	MS.gene09341:CDS	40.0%
	GTGAAGGCTTCGATTGATAA+GGG	-	chr6.2:33253763-33253782	MS.gene09341:CDS	40.0%
	TGCCACCAAACGAGAAAATT+AGG	+	chr6.2:33253665-33253684	None:intergenic	40.0%
!	TTTTGAGCCTGTACAGATTC+AGG	-	chr6.2:33253786-33253805	MS.gene09341:CDS	40.0%
!!	ACTCCAGCTTTATTAAGGAC+TGG	+	chr6.2:33253370-33253389	None:intergenic	40.0%
	ACGCCAAACCAGAGAAGAAA+TGG	-	chr6.2:33253701-33253720	MS.gene09341:CDS	45.0%
	ACTGATATTGGATGCCGTGA+AGG	-	chr6.2:33253747-33253766	MS.gene09341:CDS	45.0%
	AGAAAACGATCTACCTCAGG+AGG	-	chr6.2:33253627-33253646	MS.gene09341:CDS	45.0%
	ATCGTTGGCCATTTCTTCTC+TGG	+	chr6.2:33253712-33253731	None:intergenic	45.0%
	CGTGAAGGCTTCGATTGATA+AGG	-	chr6.2:33253762-33253781	MS.gene09341:CDS	45.0%
	GAGCCAGTCCTTAATAAAGC+TGG	-	chr6.2:33253364-33253383	MS.gene09341:CDS	45.0%
	GCTGGAGTGATAGTATCTTG+TGG	-	chr6.2:33253382-33253401	MS.gene09341:CDS	45.0%
	TGGCCATTTCTTCTCTGGTT+TGG	+	chr6.2:33253707-33253726	None:intergenic	45.0%
	GTTACCATAAGAGCCTCCTG+AGG	+	chr6.2:33253643-33253662	None:intergenic	50.0%
	TCTACCTCAGGAGGCTCTTA+TGG	-	chr6.2:33253636-33253655	MS.gene09341:CDS	50.0%
!!	ATTAAGGACTGGCTCGCGCG+TGG	+	chr6.2:33253359-33253378	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	33253346	33253813	33253346	ID=MS.gene09341
chr6.2	mRNA	33253346	33253813	33253346	ID=MS.gene09341.t1;Parent=MS.gene09341
chr6.2	exon	33253546	33253813	33253546	ID=MS.gene09341.t1.exon1;Parent=MS.gene09341.t1
chr6.2	CDS	33253546	33253813	33253546	ID=cds.MS.gene09341.t1;Parent=MS.gene09341.t1
chr6.2	exon	33253346	33253473	33253346	ID=MS.gene09341.t1.exon2;Parent=MS.gene09341.t1
chr6.2	CDS	33253346	33253473	33253346	ID=cds.MS.gene09341.t1;Parent=MS.gene09341.t1

Gene Sequence

>MS.gene09341

ATGGTATTAGCCACGCGCGAGCCAGTCCTTAATAAAGCTGGAGTGATAGTATCTTGTGGTGCTTCTTTATATTGGGAAGATGGAAGAGTAGCAACATTCTATTGCTCTTTCTTATCTAACCTAACAATGGATATAACTGCTGTTGGGACAAAAGGAACACTTCATGTTCATGATTTTATCATCCCTTATGAAGAAAAGAAAGCATCATTTTATGCAGCTTCAGAATCAAGCTTTGATGATCTTGTCACTAGATGGGGTTCGCAGCCGAGTAAGCATGTAATAGAAAACGATCTACCTCAGGAGGCTCTTATGGTAACCTAATTTTCTCGTTTGGTGGCAGAAATCAAATTCAAAAACGCCAAACCAGAGAAGAAATGGCCAACGATTAGTAGGAAAACACAACTGATATTGGATGCCGTGAAGGCTTCGATTGATAAGGGTTTTGAGCCTGTACAGATTCAGGAATGA

Protein sequence

>MS.gene09341.t1

MVLATREPVLNKAGVIVSCGASLYWEDGRVATFYCSFLSNLTMDITAVGTKGTLHVHDFIIPYEEKKASFYAASESSFDDLVTRWGSQPKIKFKNAKPEKKWPTISRKTQLILDAVKASIDKGFEPVQIQE