AlfalfaGEDB | MS.gene09480

M. sativa cultivar XinJiangDaYe / MS.gene09480

KEGG: K03695
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00004.32

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09480.t1	RZC10590.1	72.6	179	44	2	6	179	130	308	1.90E-59	238.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09480.t1	P42730	71.5	179	46	3	6	179	129	307	1.2e-60	234.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09480.t1	A0A0R0K0U9	72.6	179	44	2	6	179	130	308	1.4e-59	238.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053266	MS.gene09480	0.814388	1.58E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09480.t1	MTR_4g109450	73.743	179	42	3	6	179	129	307	2.98e-76	244
MS.gene09480.t1	MTR_4g109450	73.743	179	42	3	6	179	129	307	6.91e-76	244
MS.gene09480.t1	MTR_7g028970	67.039	179	54	3	6	179	129	307	5.83e-69	225
MS.gene09480.t1	MTR_7g012820	59.060	149	55	3	37	179	240	388	8.09e-51	175
MS.gene09480.t1	MTR_3g106160	55.215	163	67	3	23	179	234	396	1.02e-50	174
MS.gene09480.t1	MTR_7g012820	59.060	149	55	3	37	179	169	317	1.47e-50	174
MS.gene09480.t1	MTR_6g013660	58.389	149	56	3	37	179	236	384	2.78e-50	173
MS.gene09480.t1	MTR_3g102830	47.205	161	78	4	25	179	245	404	2.37e-41	148
MS.gene09480.t1	MTR_8g100040	47.205	161	78	4	25	179	244	403	5.88e-41	147
MS.gene09480.t1	MTR_3g498725	47.468	158	76	4	28	179	243	399	4.23e-40	144
MS.gene09480.t1	MTR_6g048860	41.781	146	78	4	25	164	121	265	1.41e-32	119
MS.gene09480.t1	MTR_3g098310	43.421	152	74	6	39	179	297	447	5.98e-27	106
MS.gene09480.t1	MTR_3g098310	43.421	152	74	6	39	179	297	447	6.41e-27	106
MS.gene09480.t1	MTR_3g461000	38.356	146	82	5	40	179	172	315	5.50e-23	95.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09480.t1	AT1G74310	71.508	179	46	3	6	179	129	307	1.79e-75	241
MS.gene09480.t1	AT1G74310	71.508	179	46	3	6	179	129	307	6.38e-75	241
MS.gene09480.t1	AT4G14670	53.371	178	69	2	6	179	105	272	1.90e-53	179
MS.gene09480.t1	AT2G25140	54.601	163	68	3	23	179	225	387	3.65e-51	176
MS.gene09480.t1	AT5G15450	59.060	149	55	3	37	179	234	382	1.70e-50	174
MS.gene09480.t1	AT5G50920	43.169	183	93	5	7	179	220	401	5.33e-41	147
MS.gene09480.t1	AT3G48870	50.340	147	66	4	39	179	277	422	6.10e-41	147
MS.gene09480.t1	AT3G48870	50.340	147	66	4	39	179	277	422	6.10e-41	147
MS.gene09480.t1	AT3G48870	50.340	147	66	4	39	179	277	422	6.10e-41	147
MS.gene09480.t1	AT3G48870	50.340	147	66	4	39	179	246	391	7.04e-41	146
MS.gene09480.t1	AT5G51070	45.395	152	71	5	39	179	270	420	8.17e-32	120
MS.gene09480.t1	AT3G45450	41.353	133	67	4	50	178	149	274	2.70e-24	97.4

Find 30 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGAAATCCATCTTGTTCT+TGG	0.253712	6.4:-24413335	MS.gene09480:CDS
GGGAAGCTTGACTCTGTTAT+TGG	0.260259	6.4:-24413667	MS.gene09480:CDS
AAGAACAATCTTATTCTTGT+AGG	0.288251	6.4:-24413595	MS.gene09480:CDS
ACAGATATTTGCTTTAGATA+TGG	0.312597	6.4:-24413462	MS.gene09480:intron
AAGAAAGTTGAAAGTGCTTT+TGG	0.322493	6.4:-24413745	MS.gene09480:CDS
GGTAGAAAAGATCTTGAAAA+TGG	0.347072	6.4:-24413515	MS.gene09480:intron
CAGATATTTGCTTTAGATAT+GGG	0.353602	6.4:-24413461	MS.gene09480:CDS
TTGACAAGCTTTATCGTAAA+GGG	0.387520	6.4:-24413768	MS.gene09480:CDS
GATATGGGCGCATTAGTTGC+TGG	0.417982	6.4:-24413446	MS.gene09480:CDS
AGCGATTTGGTTGAACGAGC+AGG	0.436560	6.4:-24413688	MS.gene09480:CDS
CTTATTCTTGTAGGAGAGCC+AGG	0.440113	6.4:-24413586	MS.gene09480:CDS
CTTGGTGTTGGTAGAATTGA+AGG	0.450460	6.4:-24413317	MS.gene09480:CDS
AAGTGGAAGAAGCTGAAGGA+AGG	0.467536	6.4:-24413373	MS.gene09480:CDS
ATCCATCTTGTTCTTGGTGT+TGG	0.467674	6.4:-24413329	MS.gene09480:CDS
GTTGACAAGCTTTATCGTAA+AGG	0.476986	6.4:-24413769	MS.gene09480:CDS
CTTGTAGGAGAGCCAGGTGT+TGG	0.507596	6.4:-24413580	MS.gene09480:CDS
TGGTAGAATTGAAGGATCAA+TGG	0.532319	6.4:-24413309	MS.gene09480:CDS
AGTGGAAGAAGCTGAAGGAA+GGG	0.534741	6.4:-24413372	MS.gene09480:CDS
TTGTTGAAGGGTTGTCTCAA+AGG	0.538062	6.4:-24413546	MS.gene09480:CDS
TATCCACCACGATCAAGCAT+TGG	0.546166	6.4:+24413266	None:intergenic
ATGTACAAAAGCTTTGAAGT+TGG	0.555488	6.4:-24413820	MS.gene09480:CDS
GCGATTTGGTTGAACGAGCA+GGG	0.569358	6.4:-24413687	MS.gene09480:CDS
AAAGAAGTGGAAGAAGCTGA+AGG	0.582184	6.4:-24413377	MS.gene09480:CDS
GTTGCTGGTGCTAAGAGCAA+TGG	0.583595	6.4:-24413431	MS.gene09480:CDS
TGAAGGGTTGTCTCAAAGGA+TGG	0.595540	6.4:-24413542	MS.gene09480:CDS
GTTGTCTCAAAGGATGGTAG+AGG	0.597830	6.4:-24413536	MS.gene09480:CDS
TACCAACACCAAGAACAAGA+TGG	0.602841	6.4:+24413327	None:intergenic
GACGTGATCAAGAGATAGAA+CGG	0.608823	6.4:-24413645	MS.gene09480:CDS
AAACCAATGCTTGATCGTGG+TGG	0.617029	6.4:-24413269	MS.gene09480:CDS
CTCAAACCAATGCTTGATCG+TGG	0.625491	6.4:-24413272	MS.gene09480:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTCAAGTTTTGAAAAAATA+TGG	-	chr6.4:24413373-24413392	MS.gene09480:CDS	15.0%
!	AAGAACAATCTTATTCTTGT+AGG	-	chr6.4:24413490-24413509	MS.gene09480:intron	25.0%
!!	ACAGATATTTGCTTTAGATA+TGG	-	chr6.4:24413623-24413642	MS.gene09480:CDS	25.0%
!!	CAGATATTTGCTTTAGATAT+GGG	-	chr6.4:24413624-24413643	MS.gene09480:CDS	25.0%
!!	TTGTCGTGATTTTATCAAAA+AGG	-	chr6.4:24413464-24413483	MS.gene09480:intron	25.0%
	AATGAAATCCATCTTGTTCT+TGG	-	chr6.4:24413750-24413769	MS.gene09480:CDS	30.0%
	GAAAATGGTAAAACAGATCT+TGG	-	chr6.4:24413585-24413604	MS.gene09480:CDS	30.0%
	GGTAGAAAAGATCTTGAAAA+TGG	-	chr6.4:24413570-24413589	MS.gene09480:CDS	30.0%
	TAAAGCAAATATCTGTGCAT+TGG	+	chr6.4:24413619-24413638	None:intergenic	30.0%
!	GAAAAAATATGGTAGCGATT+TGG	-	chr6.4:24413384-24413403	MS.gene09480:CDS	30.0%
!	GAAAAACTGTTGTTGTTGAA+GGG	-	chr6.4:24413527-24413546	MS.gene09480:CDS	30.0%
!	TTGACAAGCTTTATCGTAAA+GGG	-	chr6.4:24413317-24413336	MS.gene09480:CDS	30.0%
!!	ATGTACAAAAGCTTTGAAGT+TGG	-	chr6.4:24413265-24413284	MS.gene09480:CDS	30.0%
!!!	AAGAAAGTTGAAAGTGCTTT+TGG	-	chr6.4:24413340-24413359	MS.gene09480:CDS	30.0%
	TGGTAGAATTGAAGGATCAA+TGG	-	chr6.4:24413776-24413795	MS.gene09480:CDS	35.0%
!	GGAAAAACTGTTGTTGTTGA+AGG	-	chr6.4:24413526-24413545	MS.gene09480:CDS	35.0%
!	GTTGACAAGCTTTATCGTAA+AGG	-	chr6.4:24413316-24413335	MS.gene09480:CDS	35.0%
!!!	GAAGGAAGTTTTGAAAGAAG+TGG	-	chr6.4:24413695-24413714	MS.gene09480:CDS	35.0%
	AAAGAAGTGGAAGAAGCTGA+AGG	-	chr6.4:24413708-24413727	MS.gene09480:CDS	40.0%
	GACGTGATCAAGAGATAGAA+CGG	-	chr6.4:24413440-24413459	MS.gene09480:CDS	40.0%
	TACCAACACCAAGAACAAGA+TGG	+	chr6.4:24413761-24413780	None:intergenic	40.0%
	TTGTTGAAGGGTTGTCTCAA+AGG	-	chr6.4:24413539-24413558	MS.gene09480:CDS	40.0%
!	ACAACAGTTTTTCCAACACC+TGG	+	chr6.4:24413520-24413539	None:intergenic	40.0%
!	ATCCATCTTGTTCTTGGTGT+TGG	-	chr6.4:24413756-24413775	MS.gene09480:CDS	40.0%
!!	AGATTTTGAAGAGAGGCTGA+AGG	-	chr6.4:24413677-24413696	MS.gene09480:CDS	40.0%
!!	CTTGGTGTTGGTAGAATTGA+AGG	-	chr6.4:24413768-24413787	MS.gene09480:CDS	40.0%
!!	GCAATGGAGATTTTGAAGAG+AGG	-	chr6.4:24413670-24413689	MS.gene09480:CDS	40.0%
	AAACCAATGCTTGATCGTGG+TGG	-	chr6.4:24413816-24413835	MS.gene09480:CDS	45.0%
	AAGTGGAAGAAGCTGAAGGA+AGG	-	chr6.4:24413712-24413731	MS.gene09480:CDS	45.0%
	AGTGGAAGAAGCTGAAGGAA+GGG	-	chr6.4:24413713-24413732	MS.gene09480:CDS	45.0%
	CTCAAACCAATGCTTGATCG+TGG	-	chr6.4:24413813-24413832	MS.gene09480:CDS	45.0%
	CTTATTCTTGTAGGAGAGCC+AGG	-	chr6.4:24413499-24413518	MS.gene09480:intron	45.0%
	GGGAAGCTTGACTCTGTTAT+TGG	-	chr6.4:24413418-24413437	MS.gene09480:CDS	45.0%
	GTTGTCTCAAAGGATGGTAG+AGG	-	chr6.4:24413549-24413568	MS.gene09480:CDS	45.0%
	TATCCACCACGATCAAGCAT+TGG	+	chr6.4:24413822-24413841	None:intergenic	45.0%
	TGAAGGGTTGTCTCAAAGGA+TGG	-	chr6.4:24413543-24413562	MS.gene09480:CDS	45.0%
	GATATGGGCGCATTAGTTGC+TGG	-	chr6.4:24413639-24413658	MS.gene09480:CDS	50.0%
!	GTTGCTGGTGCTAAGAGCAA+TGG	-	chr6.4:24413654-24413673	MS.gene09480:CDS	50.0%
!!	AGCGATTTGGTTGAACGAGC+AGG	-	chr6.4:24413397-24413416	MS.gene09480:CDS	50.0%
!!	GCGATTTGGTTGAACGAGCA+GGG	-	chr6.4:24413398-24413417	MS.gene09480:CDS	50.0%
	CTTGTAGGAGAGCCAGGTGT+TGG	-	chr6.4:24413505-24413524	MS.gene09480:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	24413265	24413842	24413265	ID=MS.gene09480
chr6.4	mRNA	24413265	24413842	24413265	ID=MS.gene09480.t1;Parent=MS.gene09480
chr6.4	exon	24413516	24413842	24413516	ID=MS.gene09480.t1.exon1;Parent=MS.gene09480.t1
chr6.4	CDS	24413516	24413842	24413516	ID=cds.MS.gene09480.t1;Parent=MS.gene09480.t1
chr6.4	exon	24413265	24413480	24413265	ID=MS.gene09480.t1.exon2;Parent=MS.gene09480.t1
chr6.4	CDS	24413265	24413480	24413265	ID=cds.MS.gene09480.t1;Parent=MS.gene09480.t1

Gene Sequence

>MS.gene09480

ATGTACAAAAGCTTTGAAGTTGGTGTCGCTGTTTCTAGAGTAAAATCAGAAGTTGACAAGCTTTATCGTAAAGGGAAGAAAGTTGAAAGTGCTTTTGGTGATACAAATTTTCAAGTTTTGAAAAAATATGGTAGCGATTTGGTTGAACGAGCAGGGAAGCTTGACTCTGTTATTGGACGTGATCAAGAGATAGAACGGCTTGTCGTGATTTTATCAAAAAGGAATAAGAACAATCTTATTCTTGTAGGAGAGCCAGGTGTTGGAAAAACTGTTGTTGTTGAAGGGTTGTCTCAAAGGATGGTAGAGGTAGAAAAGATCTTGAAAATGGTAAAACAGATCTTGGAAATCTTGCCAATGCACAGATATTTGCTTTAGATATGGGCGCATTAGTTGCTGGTGCTAAGAGCAATGGAGATTTTGAAGAGAGGCTGAAGGAAGTTTTGAAAGAAGTGGAAGAAGCTGAAGGAAGGGTAATTGTTTTCATCAATGAAATCCATCTTGTTCTTGGTGTTGGTAGAATTGAAGGATCAATGGATGCTGCTAATCTTCTCAAACCAATGCTTGATCGTGGTGGATAA

Protein sequence

>MS.gene09480.t1

MYKSFEVGVAVSRVKSEVDKLYRKGKKVESAFGDTNFQVLKKYGSDLVERAGKLDSVIGRDQEIERLVVILSKRNKNNLILVGEPGVGKTVVVEGLSQRMVEVEKILKMIFALDMGALVAGAKSNGDFEERLKEVLKEVEEAEGRVIVFINEIHLVLGVGRIEGSMDAANLLKPMLDRGG