AlfalfaGEDB | MS.gene09654

M. sativa cultivar XinJiangDaYe / MS.gene09654

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	XP_003611481.1	96.7	180	5	1	1	180	73	251	7.80E-93	349.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	O80942	72.2	54	11	1	5	54	34	87	9.6e-15	81.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	D7R6G3	96.7	180	5	1	1	180	73	251	5.6e-93	349.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene09654.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059669	MS.gene09654	0.909963	3.18E-82	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	MTR_5g014400	96.667	180	5	1	1	180	73	251	2.42e-127	358
MS.gene09654.t1	MTR_2g076060	87.805	41	5	0	5	45	47	87	3.43e-22	89.7
MS.gene09654.t1	MTR_4g021270	37.805	164	69	6	5	140	56	214	5.88e-20	85.5
MS.gene09654.t1	MTR_3g068095	86.842	38	5	0	5	42	52	89	6.24e-19	81.6
MS.gene09654.t1	MTR_5g080660	74.419	43	11	0	3	45	42	84	9.36e-19	80.9
MS.gene09654.t1	MTR_1g070265	80.000	40	8	0	3	42	30	69	1.82e-18	79.0
MS.gene09654.t1	MTR_1g070250	31.977	172	97	5	8	177	38	191	1.19e-14	68.9
MS.gene09654.t1	MTR_7g063220	76.471	34	8	0	8	41	24	57	5.53e-14	65.9
MS.gene09654.t1	MTR_4g064873	32.749	171	85	4	7	177	20	160	6.48e-14	66.6
MS.gene09654.t1	MTR_1g075430	31.551	187	88	9	8	174	18	184	1.80e-12	63.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	AT4G17810	41.885	191	71	7	2	177	38	203	4.52e-31	112
MS.gene09654.t1	AT4G17810	39.674	184	55	9	2	177	38	173	2.07e-23	91.7
MS.gene09654.t1	AT5G06070	37.306	193	81	8	5	177	50	222	2.27e-23	92.8
MS.gene09654.t1	AT2G37740	68.421	57	14	1	2	54	31	87	1.77e-20	86.7
MS.gene09654.t1	AT3G23130	79.070	43	9	0	1	43	38	80	9.95e-20	82.8
MS.gene09654.t1	AT2G42410	35.979	189	82	6	5	174	44	212	1.22e-19	82.8
MS.gene09654.t1	AT2G42410	35.979	189	82	6	5	174	44	212	1.22e-19	82.8
MS.gene09654.t1	AT2G42410	67.925	53	17	0	5	57	70	122	2.00e-19	82.8
MS.gene09654.t1	AT3G09290	31.792	173	70	7	8	174	33	163	3.45e-13	64.7
MS.gene09654.t1	AT3G53820	63.889	36	13	0	7	42	18	53	3.43e-11	58.5

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Find 39 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGCAGGTATAGGACCT+TGG	0.620732	5.2:-6355222	None:intergenic
AGGTGCAAGTAGCATGTTGA+TGG	0.551205	5.2:-6355647	None:intergenic
ATATTTATGAATGGTGATGA+AGG	0.596989	5.2:-6355354	None:intergenic
ATGACCGTTATCATCAATGG+AGG	0.643461	5.2:-6355667	None:intergenic
ATTAGGACTAGAACTATTGT+AGG	0.449818	5.2:-6355460	None:intergenic
ATTTGCATCATCAAATTATT+TGG	0.195764	5.2:-6355534	None:intergenic
CAAAAGCATTATTATTATTA+GGG	0.268434	5.2:-6355434	None:intergenic
CAAGAGCTTGTTGCTAATGC+TGG	0.417258	5.2:+6355384	MS.gene09654:CDS
CACCAAGAGCTTGAGCTGAC+CGG	0.533757	5.2:-6355260	None:intergenic
CGGTCAGCTCAAGCTCTTGG+TGG	0.515963	5.2:+6355261	MS.gene09654:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAGGACTAGAACTATTGT+AGG	-	chr5.2:6355463-6355482	None:intergenic	30.0%
GAAGGATGAGATGAATTTAA+CGG	-	chr5.2:6355339-6355358	None:intergenic	30.0%
TGCATCATCAAATTATTTGG+AGG	-	chr5.2:6355534-6355553	None:intergenic	30.0%
TGTGGAGGAATATTTATGAA+TGG	-	chr5.2:6355366-6355385	None:intergenic	30.0%
GAGATTGAGAGAAAAGTAGA+AGG	-	chr5.2:6355495-6355514	None:intergenic	35.0%
TTCAAGTGGATAATTCAGCT+AGG	+	chr5.2:6355583-6355602	MS.gene09654:CDS	35.0%
TTCATGACCGTTATCATCAA+TGG	-	chr5.2:6355673-6355692	None:intergenic	35.0%
ATGACCGTTATCATCAATGG+AGG	-	chr5.2:6355670-6355689	None:intergenic	40.0%
GAGATGAATTTAACGGTGGT+TGG	-	chr5.2:6355332-6355351	None:intergenic	40.0%
GGATGAGATGAATTTAACGG+TGG	-	chr5.2:6355336-6355355	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	6355204	6355746	6355204	ID=MS.gene09654
chr5.2	mRNA	6355204	6355746	6355204	ID=MS.gene09654.t1;Parent=MS.gene09654
chr5.2	exon	6355204	6355746	6355204	ID=MS.gene09654.t1.exon1;Parent=MS.gene09654.t1
chr5.2	CDS	6355204	6355746	6355204	ID=cds.MS.gene09654.t1;Parent=MS.gene09654.t1

Gene Sequence

>MS.gene09654

ATGAACACAACATGGCCACCAAGGTCCTATACCTGCACTTTTTGCAGAAGAGAGTTCCGGTCAGCTCAAGCTCTTGGTGGTCACATGAATGTTCACCGCCGCGACCGTGCTCGTCTCCATCAAAGCCAACCACCGTTAAATTCATCTCATCCTTCATCACCATTCATAAATATTCCTCCACAAGAGCTTGTTGCTAATGCTGGATTGTGCCTTCTTTACCATTTACCAAACCCTAATAATAATAATGCTTTTGCTTCCTACAATAGTTCTAGTCCTAATGGAGAATCTCCTTCTACTTTTCTCTCAATCTCATCATCAACATCTTATCCTCCAAATAATTTGATGATGCAAATGCAAACTTGTTCTCCATCTTTTAATTTTCAAGTGGATAATTCAGCTAGGTTGATCAATAATAGCATTTCTTCTTTTTCTAGCAAAGTGGACCATCAACATGCTACTTGCACCTCCATTGATGATAACGGTCATGAAATTGAAGAGCTTGATCTTGAGCTCCGTTTAGGGAACAAGCCAACACCAACTTGA

Protein sequence

>MS.gene09654.t1

MNTTWPPRSYTCTFCRREFRSAQALGGHMNVHRRDRARLHQSQPPLNSSHPSSPFINIPPQELVANAGLCLLYHLPNPNNNNAFASYNSSSPNGESPSTFLSISSSTSYPPNNLMMQMQTCSPSFNFQVDNSARLINNSISSFSSKVDHQHATCTSIDDNGHEIEELDLELRLGNKPTPT