AlfalfaGEDB | MS.gene09654

M. sativa cultivar XinJiangDaYe / MS.gene09654

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	XP_003611481.1	96.7	180	5	1	1	180	73	251	7.80E-93	349.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	O80942	72.2	54	11	1	5	54	34	87	9.6e-15	81.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	D7R6G3	96.7	180	5	1	1	180	73	251	5.6e-93	349.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene09654.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059669	MS.gene09654	0.909963	3.18E-82	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	MTR_5g014400	96.667	180	5	1	1	180	73	251	2.42e-127	358
MS.gene09654.t1	MTR_2g076060	87.805	41	5	0	5	45	47	87	3.43e-22	89.7
MS.gene09654.t1	MTR_4g021270	37.805	164	69	6	5	140	56	214	5.88e-20	85.5
MS.gene09654.t1	MTR_3g068095	86.842	38	5	0	5	42	52	89	6.24e-19	81.6
MS.gene09654.t1	MTR_5g080660	74.419	43	11	0	3	45	42	84	9.36e-19	80.9
MS.gene09654.t1	MTR_1g070265	80.000	40	8	0	3	42	30	69	1.82e-18	79.0
MS.gene09654.t1	MTR_1g070250	31.977	172	97	5	8	177	38	191	1.19e-14	68.9
MS.gene09654.t1	MTR_7g063220	76.471	34	8	0	8	41	24	57	5.53e-14	65.9
MS.gene09654.t1	MTR_4g064873	32.749	171	85	4	7	177	20	160	6.48e-14	66.6
MS.gene09654.t1	MTR_1g075430	31.551	187	88	9	8	174	18	184	1.80e-12	63.2
MS.gene09654.t1	MTR_1g044225	43.548	62	26	1	8	60	26	87	8.62e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09654.t1	AT4G17810	41.885	191	71	7	2	177	38	203	4.52e-31	112
MS.gene09654.t1	AT4G17810	39.674	184	55	9	2	177	38	173	2.07e-23	91.7
MS.gene09654.t1	AT5G06070	37.306	193	81	8	5	177	50	222	2.27e-23	92.8
MS.gene09654.t1	AT2G37740	68.421	57	14	1	2	54	31	87	1.77e-20	86.7
MS.gene09654.t1	AT3G23130	79.070	43	9	0	1	43	38	80	9.95e-20	82.8
MS.gene09654.t1	AT2G42410	35.979	189	82	6	5	174	44	212	1.22e-19	82.8
MS.gene09654.t1	AT2G42410	35.979	189	82	6	5	174	44	212	1.22e-19	82.8
MS.gene09654.t1	AT2G42410	67.925	53	17	0	5	57	70	122	2.00e-19	82.8
MS.gene09654.t1	AT3G09290	31.792	173	70	7	8	174	33	163	3.45e-13	64.7
MS.gene09654.t1	AT3G53820	63.889	36	13	0	7	42	18	53	3.43e-11	58.5

Find 39 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAAGCATTATTATTATT+AGG	0.154031	5.2:-6355435	None:intergenic
TATTATTAGGGTTTGGTAAA+TGG	0.178618	5.2:-6355422	None:intergenic
ATTTGCATCATCAAATTATT+TGG	0.195764	5.2:-6355534	None:intergenic
CAAAAGCATTATTATTATTA+GGG	0.268434	5.2:-6355434	None:intergenic
TTAACGGTGGTTGGCTTTGA+TGG	0.294750	5.2:-6355320	None:intergenic
CTTGATCTTGAGCTCCGTTT+AGG	0.296503	5.2:+6355702	MS.gene09654:CDS
TACAATAGTTCTAGTCCTAA+TGG	0.356996	5.2:+6355462	MS.gene09654:CDS
TTGATCTTGAGCTCCGTTTA+GGG	0.364351	5.2:+6355703	MS.gene09654:CDS
GAGATTGAGAGAAAAGTAGA+AGG	0.386084	5.2:-6355492	None:intergenic
GTAGAAGGAGATTCTCCATT+AGG	0.392764	5.2:-6355477	None:intergenic
TTCATGACCGTTATCATCAA+TGG	0.401936	5.2:-6355670	None:intergenic
GCATTATTATTATTAGGGTT+TGG	0.403821	5.2:-6355429	None:intergenic
CAAGAGCTTGTTGCTAATGC+TGG	0.417258	5.2:+6355384	MS.gene09654:CDS
GTGTTGGCTTGTTCCCTAAA+CGG	0.435644	5.2:-6355716	None:intergenic
TCCACTTGAAAATTAAAAGA+TGG	0.441538	5.2:-6355570	None:intergenic
ATTAGGACTAGAACTATTGT+AGG	0.449818	5.2:-6355460	None:intergenic
TTCCGGTCAGCTCAAGCTCT+TGG	0.471669	5.2:+6355258	MS.gene09654:CDS
GAGATGAATTTAACGGTGGT+TGG	0.484111	5.2:-6355329	None:intergenic
TGCATCATCAAATTATTTGG+AGG	0.491401	5.2:-6355531	None:intergenic
GGTTTGGTAAATGGTAAAGA+AGG	0.506848	5.2:-6355413	None:intergenic
CGGTCAGCTCAAGCTCTTGG+TGG	0.515963	5.2:+6355261	MS.gene09654:CDS
GATGGAGACGAGCACGGTCG+CGG	0.516231	5.2:-6355302	None:intergenic
CACCAAGAGCTTGAGCTGAC+CGG	0.533757	5.2:-6355260	None:intergenic
TGTGGAGGAATATTTATGAA+TGG	0.545539	5.2:-6355363	None:intergenic
GAAGGATGAGATGAATTTAA+CGG	0.547030	5.2:-6355336	None:intergenic
AGGTGCAAGTAGCATGTTGA+TGG	0.551205	5.2:-6355647	None:intergenic
TGCACCTCCATTGATGATAA+CGG	0.570866	5.2:+6355663	MS.gene09654:CDS
GCATTAGCAACAAGCTCTTG+TGG	0.586992	5.2:-6355381	None:intergenic
GGCTTTGATGGAGACGAGCA+CGG	0.589738	5.2:-6355308	None:intergenic
ATATTTATGAATGGTGATGA+AGG	0.596989	5.2:-6355354	None:intergenic
GTGCAGGTATAGGACCTTGG+TGG	0.606792	5.2:-6355219	None:intergenic
AAAGTGCAGGTATAGGACCT+TGG	0.620732	5.2:-6355222	None:intergenic
TTCAAGTGGATAATTCAGCT+AGG	0.628424	5.2:+6355583	MS.gene09654:CDS
ATGACCGTTATCATCAATGG+AGG	0.643461	5.2:-6355667	None:intergenic
TGAACACAACATGGCCACCA+AGG	0.646071	5.2:+6355205	MS.gene09654:CDS
GGAGACGAGCACGGTCGCGG+CGG	0.650175	5.2:-6355299	None:intergenic
TTAGCAACAAGCTCTTGTGG+AGG	0.690594	5.2:-6355378	None:intergenic
GGAATATTATGAACACAACA+TGG	0.697427	5.2:+6355196	None:intergenic
GGATGAGATGAATTTAACGG+TGG	0.714956	5.2:-6355333	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CAAAAGCATTATTATTATTA+GGG	-	chr5.2:6355437-6355456	None:intergenic	15.0%
!!	ATTTGCATCATCAAATTATT+TGG	-	chr5.2:6355537-6355556	None:intergenic	20.0%
!!!	GCAAAAGCATTATTATTATT+AGG	-	chr5.2:6355438-6355457	None:intergenic	20.0%
!	GCATTATTATTATTAGGGTT+TGG	-	chr5.2:6355432-6355451	None:intergenic	25.0%
!	TCCACTTGAAAATTAAAAGA+TGG	-	chr5.2:6355573-6355592	None:intergenic	25.0%
!!	ATATTTATGAATGGTGATGA+AGG	-	chr5.2:6355357-6355376	None:intergenic	25.0%
!!	TATTATTAGGGTTTGGTAAA+TGG	-	chr5.2:6355425-6355444	None:intergenic	25.0%
!!!	TCCATCTTTTAATTTTCAAG+TGG	+	chr5.2:6355569-6355588	MS.gene09654:CDS	25.0%
	ATTAGGACTAGAACTATTGT+AGG	-	chr5.2:6355463-6355482	None:intergenic	30.0%
	GAAGGATGAGATGAATTTAA+CGG	-	chr5.2:6355339-6355358	None:intergenic	30.0%
	TGCATCATCAAATTATTTGG+AGG	-	chr5.2:6355534-6355553	None:intergenic	30.0%
	TGTGGAGGAATATTTATGAA+TGG	-	chr5.2:6355366-6355385	None:intergenic	30.0%
!!	TACAATAGTTCTAGTCCTAA+TGG	+	chr5.2:6355462-6355481	MS.gene09654:CDS	30.0%
!!	TTCTTCTTTTTCTAGCAAAG+TGG	+	chr5.2:6355623-6355642	MS.gene09654:CDS	30.0%
	GAGATTGAGAGAAAAGTAGA+AGG	-	chr5.2:6355495-6355514	None:intergenic	35.0%
	TTCAAGTGGATAATTCAGCT+AGG	+	chr5.2:6355583-6355602	MS.gene09654:CDS	35.0%
	TTCATGACCGTTATCATCAA+TGG	-	chr5.2:6355673-6355692	None:intergenic	35.0%
!	GGTTTGGTAAATGGTAAAGA+AGG	-	chr5.2:6355416-6355435	None:intergenic	35.0%
	ATGACCGTTATCATCAATGG+AGG	-	chr5.2:6355670-6355689	None:intergenic	40.0%
	GAGATGAATTTAACGGTGGT+TGG	-	chr5.2:6355332-6355351	None:intergenic	40.0%
	GGATGAGATGAATTTAACGG+TGG	-	chr5.2:6355336-6355355	None:intergenic	40.0%
	GTAGAAGGAGATTCTCCATT+AGG	-	chr5.2:6355480-6355499	None:intergenic	40.0%
	TTGATCTTGAGCTCCGTTTA+GGG	+	chr5.2:6355703-6355722	MS.gene09654:CDS	40.0%
!	CTTTTTGCAGAAGAGAGTTC+CGG	+	chr5.2:6355241-6355260	MS.gene09654:CDS	40.0%
!	TCTGCAAAAAGTGCAGGTAT+AGG	-	chr5.2:6355232-6355251	None:intergenic	40.0%
!	TGCACCTCCATTGATGATAA+CGG	+	chr5.2:6355663-6355682	MS.gene09654:CDS	40.0%
	AAAGTGCAGGTATAGGACCT+TGG	-	chr5.2:6355225-6355244	None:intergenic	45.0%
	AGGTGCAAGTAGCATGTTGA+TGG	-	chr5.2:6355650-6355669	None:intergenic	45.0%
	CTTGATCTTGAGCTCCGTTT+AGG	+	chr5.2:6355702-6355721	MS.gene09654:CDS	45.0%
	GCATTAGCAACAAGCTCTTG+TGG	-	chr5.2:6355384-6355403	None:intergenic	45.0%
	TTAGCAACAAGCTCTTGTGG+AGG	-	chr5.2:6355381-6355400	None:intergenic	45.0%
!	CTCTCTTCTGCAAAAAGTGC+AGG	-	chr5.2:6355238-6355257	None:intergenic	45.0%
!!	CAAGAGCTTGTTGCTAATGC+TGG	+	chr5.2:6355384-6355403	MS.gene09654:CDS	45.0%
!!	GTGTTGGCTTGTTCCCTAAA+CGG	-	chr5.2:6355719-6355738	None:intergenic	45.0%
!!	TTAACGGTGGTTGGCTTTGA+TGG	-	chr5.2:6355323-6355342	None:intergenic	45.0%
	TGAACACAACATGGCCACCA+AGG	+	chr5.2:6355205-6355224	MS.gene09654:CDS	50.0%
	CACCAAGAGCTTGAGCTGAC+CGG	-	chr5.2:6355263-6355282	None:intergenic	55.0%
	GTGCAGGTATAGGACCTTGG+TGG	-	chr5.2:6355222-6355241	None:intergenic	55.0%
	TTCCGGTCAGCTCAAGCTCT+TGG	+	chr5.2:6355258-6355277	MS.gene09654:CDS	55.0%
!	GGCTTTGATGGAGACGAGCA+CGG	-	chr5.2:6355311-6355330	None:intergenic	55.0%
	CGGTCAGCTCAAGCTCTTGG+TGG	+	chr5.2:6355261-6355280	MS.gene09654:CDS	60.0%
	GATGGAGACGAGCACGGTCG+CGG	-	chr5.2:6355305-6355324	None:intergenic	65.0%
	GGAGACGAGCACGGTCGCGG+CGG	-	chr5.2:6355302-6355321	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	6355204	6355746	6355204	ID=MS.gene09654
chr5.2	mRNA	6355204	6355746	6355204	ID=MS.gene09654.t1;Parent=MS.gene09654
chr5.2	exon	6355204	6355746	6355204	ID=MS.gene09654.t1.exon1;Parent=MS.gene09654.t1
chr5.2	CDS	6355204	6355746	6355204	ID=cds.MS.gene09654.t1;Parent=MS.gene09654.t1

Gene Sequence

>MS.gene09654

ATGAACACAACATGGCCACCAAGGTCCTATACCTGCACTTTTTGCAGAAGAGAGTTCCGGTCAGCTCAAGCTCTTGGTGGTCACATGAATGTTCACCGCCGCGACCGTGCTCGTCTCCATCAAAGCCAACCACCGTTAAATTCATCTCATCCTTCATCACCATTCATAAATATTCCTCCACAAGAGCTTGTTGCTAATGCTGGATTGTGCCTTCTTTACCATTTACCAAACCCTAATAATAATAATGCTTTTGCTTCCTACAATAGTTCTAGTCCTAATGGAGAATCTCCTTCTACTTTTCTCTCAATCTCATCATCAACATCTTATCCTCCAAATAATTTGATGATGCAAATGCAAACTTGTTCTCCATCTTTTAATTTTCAAGTGGATAATTCAGCTAGGTTGATCAATAATAGCATTTCTTCTTTTTCTAGCAAAGTGGACCATCAACATGCTACTTGCACCTCCATTGATGATAACGGTCATGAAATTGAAGAGCTTGATCTTGAGCTCCGTTTAGGGAACAAGCCAACACCAACTTGA

Protein sequence

>MS.gene09654.t1

MNTTWPPRSYTCTFCRREFRSAQALGGHMNVHRRDRARLHQSQPPLNSSHPSSPFINIPPQELVANAGLCLLYHLPNPNNNNAFASYNSSSPNGESPSTFLSISSSTSYPPNNLMMQMQTCSPSFNFQVDNSARLINNSISSFSSKVDHQHATCTSIDDNGHEIEELDLELRLGNKPTPT