Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09723.t1 | RHN53792.1 | 90.7 | 366 | 27 | 3 | 1 | 365 | 1 | 360 | 7.80E-185 | 657.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09723.t1 | G0LD36 | 33.3 | 126 | 78 | 4 | 75 | 200 | 59 | 178 | 3.3e-06 | 55.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09723.t1 | A0A396HKC3 | 90.7 | 366 | 27 | 3 | 1 | 365 | 1 | 360 | 5.6e-185 | 657.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene09723 | 0.807496 | 4.91E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09723.t1 | MTR_5g013270 | 90.217 | 368 | 27 | 4 | 1 | 365 | 1 | 362 | 0.0 | 676 |
| MS.gene09723.t1 | MTR_5g013280 | 94.758 | 248 | 13 | 0 | 366 | 613 | 45 | 292 | 2.98e-152 | 440 |
| MS.gene09723.t1 | MTR_5g013280 | 94.323 | 229 | 13 | 0 | 366 | 594 | 45 | 273 | 4.11e-136 | 399 |
| MS.gene09723.t1 | MTR_5g013280 | 94.248 | 226 | 13 | 0 | 369 | 594 | 1 | 226 | 1.50e-134 | 393 |
| MS.gene09723.t1 | MTR_1g011660 | 28.750 | 240 | 158 | 5 | 17 | 252 | 7 | 237 | 6.75e-16 | 80.9 |
| MS.gene09723.t1 | MTR_7g114960 | 27.094 | 203 | 137 | 6 | 54 | 250 | 44 | 241 | 1.13e-13 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09723.t1 | AT5G02890 | 40.984 | 244 | 118 | 9 | 15 | 244 | 8 | 239 | 4.69e-48 | 172 |
| MS.gene09723.t1 | AT4G17540 | 41.102 | 236 | 108 | 4 | 366 | 597 | 63 | 271 | 8.75e-36 | 136 |
Find 118 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACATCATGGAATGATTT+TGG | 0.185254 | 5.3:+6304562 | MS.gene09723:CDS |
| CCTCCTCTTGGCAGAGGTTT+TGG | 0.203119 | 5.3:+6304147 | MS.gene09723:CDS |
| GTATTTGTGCCCATTTATTA+AGG | 0.255802 | 5.3:-6304183 | None:intergenic |
| GAAATTGTGTCAAATGATTC+TGG | 0.265793 | 5.3:+6303698 | MS.gene09723:CDS |
| TAGACGAGGGTAGTTAAATA+TGG | 0.288777 | 5.3:-6304248 | None:intergenic |
| TCTCGTAGTACAATACTATT+TGG | 0.296543 | 5.3:-6303553 | None:intergenic |
| GATCATGATTGGTCTTTAAC+TGG | 0.302060 | 5.3:+6303590 | MS.gene09723:CDS |
| GCCACAGGTGCAGTCAGTTC+TGG | 0.319464 | 5.3:+6307507 | MS.gene09723:CDS |
| TTCATATAGTTGAGGAAGAT+TGG | 0.320215 | 5.3:+6306419 | MS.gene09723:intron |
| CAAGTTCTTCAATCATTTGC+TGG | 0.323313 | 5.3:-6309237 | None:intergenic |
| TGGATACACTTTGAATGAAT+TGG | 0.325702 | 5.3:+6304516 | MS.gene09723:CDS |
| GGGGAGAGAATTGAGGAAAT+TGG | 0.331279 | 5.3:-6303841 | None:intergenic |
| CAGTTATCTCAAGGTCTAGT+AGG | 0.335039 | 5.3:+6307564 | MS.gene09723:CDS |
| TTCTGGAATTCGACTCTTAG+AGG | 0.337713 | 5.3:+6303715 | MS.gene09723:CDS |
| GTTGGATGTGGAGTTGGTTT+CGG | 0.345001 | 5.3:+6306464 | MS.gene09723:CDS |
| GTCACTTGCTAGGGCCTTGT+TGG | 0.347732 | 5.3:+6303625 | MS.gene09723:CDS |
| ACAAAGATGATGATGAAGTT+TGG | 0.354809 | 5.3:+6307429 | MS.gene09723:CDS |
| ACCCGGATTAAACGGTAAAC+TGG | 0.357165 | 5.3:-6307460 | None:intergenic |
| AGAACTAGCTTCAAGTTTGC+TGG | 0.358017 | 5.3:-6309314 | None:intergenic |
| TCCCAGTTTACCGTTTAATC+CGG | 0.367717 | 5.3:+6307458 | MS.gene09723:CDS |
| GTTAATGTTTCTTGTTGGAT+TGG | 0.387714 | 5.3:+6304622 | MS.gene09723:CDS |
| ATCCAACAAGAAACATTAAC+AGG | 0.394612 | 5.3:-6304619 | None:intergenic |
| TTCTACCTCAATGACCTTCA+AGG | 0.395416 | 5.3:-6304479 | None:intergenic |
| AAACTTGAAGCTAGTTCTTC+AGG | 0.400339 | 5.3:+6309319 | MS.gene09723:CDS |
| TCAACCAATGAACATTGGTT+TGG | 0.414979 | 5.3:+6307590 | MS.gene09723:CDS |
| TCTGCAAAATCCACTGTTGA+AGG | 0.419732 | 5.3:+6307692 | MS.gene09723:CDS |
| AAAGCCAAACCAATGTTCAT+TGG | 0.422194 | 5.3:-6307594 | None:intergenic |
| TGCACCTGTGGCACTTTGTA+AGG | 0.422373 | 5.3:-6307496 | None:intergenic |
| GTGGGTGTTGGATGTGGAGT+TGG | 0.433318 | 5.3:+6306458 | MS.gene09723:CDS |
| TTGACAAATTTGTCGTGGTT+CGG | 0.435141 | 5.3:-6303486 | None:intergenic |
| GGCAATGCACCCGGATTAAA+CGG | 0.440473 | 5.3:-6307468 | None:intergenic |
| AGCCTGTTAATGTTTCTTGT+TGG | 0.444146 | 5.3:+6304617 | MS.gene09723:CDS |
| ATCAAGTTATGAGTGCTACT+AGG | 0.451482 | 5.3:+6306275 | MS.gene09723:CDS |
| AAATTATTTGCATTACATCA+TGG | 0.460101 | 5.3:+6304551 | MS.gene09723:CDS |
| ATGAGAGCTTACTGATTCGC+TGG | 0.460415 | 5.3:-6307730 | None:intergenic |
| ATGATTGGTCTTTAACTGGT+TGG | 0.465585 | 5.3:+6303594 | MS.gene09723:CDS |
| CATTAGTTGTAGCCTCCTCT+TGG | 0.466773 | 5.3:+6304135 | MS.gene09723:CDS |
| TCAAGTTATGAGTGCTACTA+GGG | 0.471040 | 5.3:+6306276 | MS.gene09723:CDS |
| TGTGGAGTTGGTTTCGGTCA+TGG | 0.472934 | 5.3:+6306470 | MS.gene09723:CDS |
| ACAGGACTTGCTGTGAAACC+TGG | 0.477065 | 5.3:+6307280 | MS.gene09723:intron |
| GGTTGTTAAAGAATCCTTGA+AGG | 0.479617 | 5.3:+6304465 | MS.gene09723:CDS |
| GGATTGTGGAGTCACTTGCT+AGG | 0.481051 | 5.3:+6303615 | MS.gene09723:CDS |
| GTCTTTAACTGGTTGGATTG+TGG | 0.481596 | 5.3:+6303601 | MS.gene09723:CDS |
| GTTGGTTTCGGTCATGGTGT+TGG | 0.481887 | 5.3:+6306476 | MS.gene09723:CDS |
| TTGTAGCCTCCTCTTGGCAG+AGG | 0.485455 | 5.3:+6304141 | MS.gene09723:CDS |
| ATTGAAGTGGGTGTTGGATG+TGG | 0.485704 | 5.3:+6306452 | MS.gene09723:CDS |
| TGGAGGAAAATGAGAAGCTT+CGG | 0.486885 | 5.3:+6309260 | MS.gene09723:CDS |
| CAGCTACAATTTGTGAGCTA+TGG | 0.487162 | 5.3:-6303436 | None:intergenic |
| CCTCCTTCTCCCTTCAACAG+TGG | 0.488432 | 5.3:-6307702 | None:intergenic |
| TGTATTCAAAATCACTACTA+AGG | 0.493290 | 5.3:+6304342 | MS.gene09723:CDS |
| ACTTCAGCCGACTCTACATA+TGG | 0.500649 | 5.3:+6307639 | MS.gene09723:CDS |
| GAAACAAAGAGGACAATTGA+GGG | 0.502828 | 5.3:+6303524 | MS.gene09723:CDS |
| TTCGGTCATGGTGTTGGAGT+TGG | 0.502955 | 5.3:+6306482 | MS.gene09723:CDS |
| CCCAGTTTACCGTTTAATCC+GGG | 0.504429 | 5.3:+6307459 | MS.gene09723:CDS |
| TGAATTTGATTCAACACCCC+AGG | 0.508485 | 5.3:-6307298 | None:intergenic |
| CAAAACAAACATTCTCCTCT+AGG | 0.508628 | 5.3:-6304681 | None:intergenic |
| TAAAAGAGGGGAGAGAATTG+AGG | 0.510849 | 5.3:-6303848 | None:intergenic |
| TTGGATCATCCTCTACTAGC+TGG | 0.512145 | 5.3:+6303644 | MS.gene09723:CDS |
| CACTTCTTCAATGCAAAACA+TGG | 0.518960 | 5.3:-6304401 | None:intergenic |
| GATTGTGGAGTCACTTGCTA+GGG | 0.520946 | 5.3:+6303616 | MS.gene09723:CDS |
| GATCATGTGCATTGCAATGT+TGG | 0.525215 | 5.3:+6303404 | MS.gene09723:CDS |
| TGAAACAAAGAGGACAATTG+AGG | 0.527404 | 5.3:+6303523 | MS.gene09723:CDS |
| CTGCAAAATCCACTGTTGAA+GGG | 0.528070 | 5.3:+6307693 | MS.gene09723:CDS |
| AAGAATATTGAAGTGGGTGT+TGG | 0.528611 | 5.3:+6306446 | MS.gene09723:CDS |
| TTCAAGAGGAGGATCATGAT+TGG | 0.535806 | 5.3:+6303579 | MS.gene09723:CDS |
| AGAATCCTTGAAGGTCATTG+AGG | 0.548805 | 5.3:+6304474 | MS.gene09723:CDS |
| TGTTTCTTGTTGGATTGGAT+CGG | 0.549812 | 5.3:+6304627 | MS.gene09723:CDS |
| TATTTGCCTGAACATAAAAG+AGG | 0.554835 | 5.3:-6303862 | None:intergenic |
| CCCGGATTAAACGGTAAACT+GGG | 0.556288 | 5.3:-6307459 | None:intergenic |
| GCAAATGATTGAAGAACTTG+TGG | 0.559081 | 5.3:+6309240 | MS.gene09723:CDS |
| AAGGATGCTGGCAATGCACC+CGG | 0.560291 | 5.3:-6307477 | None:intergenic |
| CAGGACTTGCTGTGAAACCT+GGG | 0.561209 | 5.3:+6307281 | MS.gene09723:intron |
| AGAGAACAAAATACTTCAGA+TGG | 0.561726 | 5.3:+6309066 | MS.gene09723:CDS |
| AGTTATCTCAAGGTCTAGTA+GGG | 0.564091 | 5.3:+6307565 | MS.gene09723:CDS |
| ATTTGCCTGAACATAAAAGA+GGG | 0.568320 | 5.3:-6303861 | None:intergenic |
| CAAACATTCTCCTCTAGGCA+TGG | 0.572622 | 5.3:-6304676 | None:intergenic |
| AGAATTTGACAAATTTGTCG+TGG | 0.577657 | 5.3:-6303491 | None:intergenic |
| TGGTTGATTAAGTCCTCTGA+TGG | 0.577721 | 5.3:-6304289 | None:intergenic |
| TCCAGAACTGACTGCACCTG+TGG | 0.578803 | 5.3:-6307508 | None:intergenic |
| CTAGCTTCAAGTTTGCTGGA+TGG | 0.583563 | 5.3:-6309310 | None:intergenic |
| TGAGAGCTTACTGATTCGCT+GGG | 0.586043 | 5.3:-6307729 | None:intergenic |
| GTGGCACTTTGTAAGGATGC+TGG | 0.588800 | 5.3:-6307489 | None:intergenic |
| AATCCACTGTTGAAGGGAGA+AGG | 0.589188 | 5.3:+6307699 | MS.gene09723:CDS |
| GCATCCTTACAAAGTGCCAC+AGG | 0.589278 | 5.3:+6307492 | MS.gene09723:CDS |
| TCAGCTGATCAGTTATCTCA+AGG | 0.590242 | 5.3:+6307555 | MS.gene09723:CDS |
| TGAACTCGACCAGCTAGTAG+AGG | 0.590621 | 5.3:-6303653 | None:intergenic |
| ATATGGCAGTCGAACTGAGA+AGG | 0.597072 | 5.3:+6307656 | MS.gene09723:CDS |
| TGGAGCTAAGAATATTGAAG+TGG | 0.597204 | 5.3:+6306439 | MS.gene09723:CDS |
| GTAGAAAGTGTCACAAAGAG+TGG | 0.602074 | 5.3:+6304496 | MS.gene09723:CDS |
| CCACTGTTGAAGGGAGAAGG+AGG | 0.603974 | 5.3:+6307702 | MS.gene09723:CDS |
| GGAGCTAAGAATATTGAAGT+GGG | 0.605534 | 5.3:+6306440 | MS.gene09723:CDS |
| AAATGTGGAGGATACTCGAT+CGG | 0.614492 | 5.3:+6304112 | MS.gene09723:CDS |
| TAAAATTCAGCAAAGAATCG+TGG | 0.615035 | 5.3:+6304368 | MS.gene09723:CDS |
| AATGATTGAAGAACTTGTGG+AGG | 0.616671 | 5.3:+6309243 | MS.gene09723:CDS |
| AATAGTATTGTACTACGAGA+AGG | 0.621193 | 5.3:+6303556 | MS.gene09723:CDS |
| CTGTTGAAGGGAGAAGGAGG+AGG | 0.623525 | 5.3:+6307705 | MS.gene09723:CDS |
| TCGACTGCCATATGTAGAGT+CGG | 0.624499 | 5.3:-6307646 | None:intergenic |
| GTGACAAACTTCAAATGTGG+AGG | 0.625328 | 5.3:+6304100 | MS.gene09723:CDS |
| TGTTTGCAGATCTTTACACC+CGG | 0.625864 | 5.3:+6311001 | MS.gene09723:intron |
| GGGTCTCAACCAATGAACAT+TGG | 0.632984 | 5.3:+6307585 | MS.gene09723:CDS |
| CAGGTGACAAACTTCAAATG+TGG | 0.633991 | 5.3:+6304097 | MS.gene09723:intron |
| TATTCAATGATGAAACAAAG+AGG | 0.639806 | 5.3:+6303513 | MS.gene09723:CDS |
| CCAAAACCTCTGCCAAGAGG+AGG | 0.639902 | 5.3:-6304147 | None:intergenic |
| GCTTCGGCAGATACTAGTGA+AGG | 0.640377 | 5.3:+6309276 | MS.gene09723:CDS |
| AATTCAGCAAAGAATCGTGG+AGG | 0.649632 | 5.3:+6304371 | MS.gene09723:CDS |
| GTACTACGAGAAGGTTCAAG+AGG | 0.651137 | 5.3:+6303565 | MS.gene09723:CDS |
| CTCATAACTTGATTCAACAT+CGG | 0.655826 | 5.3:-6306265 | None:intergenic |
| CATGATAGTTCCATGCCTAG+AGG | 0.656402 | 5.3:+6304666 | MS.gene09723:CDS |
| CTACGAGAAGGTTCAAGAGG+AGG | 0.659531 | 5.3:+6303568 | MS.gene09723:CDS |
| ATTCAGCAAAGAATCGTGGA+GGG | 0.660581 | 5.3:+6304372 | MS.gene09723:CDS |
| ACGAGGGTAGTTAAATATGG+AGG | 0.667410 | 5.3:-6304245 | None:intergenic |
| GGTTGATTAAGTCCTCTGAT+GGG | 0.688090 | 5.3:-6304288 | None:intergenic |
| TTTGCCTGAACATAAAAGAG+GGG | 0.694331 | 5.3:-6303860 | None:intergenic |
| TTACCAAAACCTCTGCCAAG+AGG | 0.696421 | 5.3:-6304150 | None:intergenic |
| ACGTGATGATGATGATCATG+AGG | 0.697950 | 5.3:+6303787 | MS.gene09723:CDS |
| AGGACTTGCTGTGAAACCTG+GGG | 0.707838 | 5.3:+6307282 | MS.gene09723:intron |
| TAGTAGAGGATGATCCAACA+AGG | 0.710994 | 5.3:-6303639 | None:intergenic |
| CAAGTTATGAGTGCTACTAG+GGG | 0.719794 | 5.3:+6306277 | MS.gene09723:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6303401 | 6311068 | 6303401 | ID=MS.gene09723 |
| chr5.3 | mRNA | 6303401 | 6311068 | 6303401 | ID=MS.gene09723.t1;Parent=MS.gene09723 |
| chr5.3 | exon | 6303401 | 6303871 | 6303401 | ID=MS.gene09723.t1.exon1;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6303401 | 6303871 | 6303401 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6304100 | 6304721 | 6304100 | ID=MS.gene09723.t1.exon2;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6304100 | 6304721 | 6304100 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6306257 | 6306345 | 6306257 | ID=MS.gene09723.t1.exon3;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6306257 | 6306345 | 6306257 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6306428 | 6306503 | 6306428 | ID=MS.gene09723.t1.exon4;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6306428 | 6306503 | 6306428 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6307284 | 6307333 | 6307284 | ID=MS.gene09723.t1.exon5;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6307284 | 6307333 | 6307284 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6307420 | 6307740 | 6307420 | ID=MS.gene09723.t1.exon6;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6307420 | 6307740 | 6307420 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6309049 | 6309087 | 6309049 | ID=MS.gene09723.t1.exon7;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6309049 | 6309087 | 6309049 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6309226 | 6309340 | 6309226 | ID=MS.gene09723.t1.exon8;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6309226 | 6309340 | 6309226 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| chr5.3 | exon | 6311010 | 6311068 | 6311010 | ID=MS.gene09723.t1.exon9;Parent=MS.gene09723.t1 |
| chr5.3 | CDS | 6311010 | 6311068 | 6311010 | ID=cds.MS.gene09723.t1;Parent=MS.gene09723.t1 |
| Gene Sequence |
| Protein sequence |
