Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09729.t1 | ALM55021.1 | 98.8 | 428 | 5 | 0 | 1 | 428 | 1 | 428 | 1.20E-245 | 858.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09729.t1 | Q8W5B1 | 54.3 | 433 | 161 | 8 | 1 | 428 | 1 | 401 | 1.8e-120 | 434.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09729.t1 | A0A141GTN5 | 98.8 | 428 | 5 | 0 | 1 | 428 | 1 | 428 | 8.9e-246 | 858.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052072 | MS.gene09729 | 0.802183 | 6.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09729.t1 | MTR_5g013150 | 98.598 | 428 | 6 | 0 | 1 | 428 | 51 | 478 | 0.0 | 883 |
| MS.gene09729.t1 | MTR_1g090240 | 41.520 | 171 | 95 | 1 | 254 | 419 | 496 | 666 | 1.91e-37 | 144 |
| MS.gene09729.t1 | MTR_1g090240 | 46.429 | 140 | 65 | 3 | 61 | 196 | 216 | 349 | 4.80e-27 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09729.t1 | AT4G16845 | 55.189 | 424 | 149 | 9 | 1 | 417 | 1 | 390 | 5.68e-144 | 418 |
| MS.gene09729.t1 | AT5G51230 | 55.525 | 362 | 130 | 4 | 58 | 419 | 295 | 625 | 2.40e-114 | 348 |
| MS.gene09729.t1 | AT5G51230 | 55.882 | 68 | 30 | 0 | 1 | 68 | 21 | 88 | 2.02e-12 | 69.7 |
| MS.gene09729.t1 | AT5G51230 | 55.525 | 362 | 130 | 4 | 58 | 419 | 295 | 625 | 2.40e-114 | 348 |
| MS.gene09729.t1 | AT5G51230 | 55.882 | 68 | 30 | 0 | 1 | 68 | 21 | 88 | 2.02e-12 | 69.7 |
| MS.gene09729.t1 | AT5G51230 | 55.125 | 361 | 132 | 5 | 61 | 419 | 298 | 630 | 2.64e-114 | 348 |
| MS.gene09729.t1 | AT5G51230 | 55.882 | 68 | 30 | 0 | 1 | 68 | 21 | 88 | 1.98e-12 | 69.7 |
| MS.gene09729.t1 | AT4G16845 | 50.000 | 364 | 136 | 10 | 66 | 417 | 1 | 330 | 2.63e-100 | 304 |
| MS.gene09729.t1 | AT2G35670 | 35.531 | 273 | 156 | 7 | 162 | 422 | 471 | 735 | 6.45e-44 | 164 |
| MS.gene09729.t1 | AT2G35670 | 41.089 | 202 | 106 | 7 | 20 | 212 | 65 | 262 | 4.90e-29 | 120 |
| MS.gene09729.t1 | AT4G16810 | 49.342 | 152 | 73 | 3 | 268 | 415 | 2 | 153 | 4.05e-42 | 150 |
Find 88 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGCAATGTGCGAGCTTTA+AGG | 0.189333 | 5.3:-6122322 | MS.gene09729:intron |
| ATTCATCCCGAACTTTATTT+TGG | 0.206594 | 5.3:-6116916 | MS.gene09729:intron |
| ATCTATTCAACTTTGAGTTC+TGG | 0.236833 | 5.3:-6121417 | MS.gene09729:CDS |
| TATATTGATTTGCTACATTC+AGG | 0.264863 | 5.3:+6119613 | None:intergenic |
| CCGAGAGGCTCCGTTCAAAA+TGG | 0.288773 | 5.3:-6120502 | MS.gene09729:CDS |
| TGACATATTCTCCCCTTTGC+TGG | 0.299644 | 5.3:+6119792 | None:intergenic |
| GGAAATGGTGGGGTAAAATT+TGG | 0.308662 | 5.3:-6119733 | MS.gene09729:CDS |
| GAACAACTGCAGTATAATAT+TGG | 0.316221 | 5.3:-6116493 | MS.gene09729:CDS |
| TCTATTCAACTTTGAGTTCT+GGG | 0.342392 | 5.3:-6121416 | MS.gene09729:intron |
| GCACATTGCATCAAGCAAAA+TGG | 0.350061 | 5.3:+6122333 | None:intergenic |
| TATTGGAAAACTACCAAAAC+GGG | 0.359120 | 5.3:-6116476 | MS.gene09729:CDS |
| CAGACCGTTCAGACTCAAAA+AGG | 0.365583 | 5.3:-6119546 | MS.gene09729:intron |
| ACCCCATTCTGTATGCCTTC+TGG | 0.369094 | 5.3:+6120442 | None:intergenic |
| AGATTACCCTTCTATCTTCC+AGG | 0.369139 | 5.3:+6117764 | None:intergenic |
| ATGGTCAAACTTTGGAATCA+TGG | 0.374400 | 5.3:-6116537 | MS.gene09729:CDS |
| TTCAGGGTCCTCAGAAGATT+GGG | 0.379052 | 5.3:+6119630 | None:intergenic |
| CAATGTAAATTTCTTGAAGT+TGG | 0.379544 | 5.3:-6120470 | MS.gene09729:CDS |
| AATTCACCGGTGCATAATTC+CGG | 0.383881 | 5.3:-6124917 | MS.gene09729:CDS |
| AAAAGCAGCTCATGCATCTC+TGG | 0.388906 | 5.3:-6117166 | MS.gene09729:CDS |
| TTTAACTTCTTTGCTTTAGT+AGG | 0.389750 | 5.3:+6119571 | None:intergenic |
| GATTATTCATGGTCAAACTT+TGG | 0.391223 | 5.3:-6116545 | MS.gene09729:CDS |
| ATGAGTACCAAAATAAAGTT+CGG | 0.391721 | 5.3:+6116909 | None:intergenic |
| AGAATATTGTAGAATTCAAC+AGG | 0.395051 | 5.3:+6124848 | None:intergenic |
| AATCACAAACCTTCTTCTTC+TGG | 0.397340 | 5.3:-6120636 | MS.gene09729:intron |
| CTTTCACTCACACAGAGTTC+AGG | 0.399813 | 5.3:-6118364 | MS.gene09729:intron |
| TTGCTAACCTGGAAGATAGA+AGG | 0.399917 | 5.3:-6117771 | MS.gene09729:CDS |
| TATTCTCCCCTTTGCTGGAC+AGG | 0.412431 | 5.3:+6119797 | None:intergenic |
| AAGAAGAAGGTTTGTGATTG+TGG | 0.419388 | 5.3:+6120640 | None:intergenic |
| CACCAGAAGGCATACAGAAT+GGG | 0.421835 | 5.3:-6120444 | MS.gene09729:CDS |
| GTGAGAAGAATTTGCAGAAT+GGG | 0.428493 | 5.3:-6119756 | MS.gene09729:CDS |
| ATTCAGGGTCCTCAGAAGAT+TGG | 0.434866 | 5.3:+6119629 | None:intergenic |
| CAGGTGTTGGAGATTATTCA+TGG | 0.435769 | 5.3:-6116556 | MS.gene09729:CDS |
| TGAGTACCAAAATAAAGTTC+GGG | 0.440705 | 5.3:+6116910 | None:intergenic |
| AGCTGTGATTTCTTCTTCAC+CGG | 0.443162 | 5.3:+6124898 | None:intergenic |
| TCCATGGCACTAGGATGATC+AGG | 0.457194 | 5.3:+6119709 | None:intergenic |
| GAAAGAACTGCCTCTTCTGC+AGG | 0.458363 | 5.3:+6118382 | None:intergenic |
| TGGTCAAACTTTGGAATCAT+GGG | 0.467268 | 5.3:-6116536 | MS.gene09729:CDS |
| CGAGAGGCTCCGTTCAAAAT+GGG | 0.471224 | 5.3:-6120501 | MS.gene09729:CDS |
| TTTGCTTTAGTAGGCAGAGC+AGG | 0.471731 | 5.3:+6119580 | None:intergenic |
| ATATTGATTTGCTACATTCA+GGG | 0.478951 | 5.3:+6119614 | None:intergenic |
| TTACAGAGACGTTCACTGCT+TGG | 0.482117 | 5.3:+6121297 | None:intergenic |
| ATATTGGAAAACTACCAAAA+CGG | 0.483344 | 5.3:-6116477 | MS.gene09729:CDS |
| AAACATCTCTTAAGAAAAGT+AGG | 0.489127 | 5.3:+6124212 | None:intergenic |
| TAGATATCCTGTCCAGCAAA+GGG | 0.491609 | 5.3:-6119804 | MS.gene09729:CDS |
| AACACTTGTTCCAGTGCCAT+GGG | 0.492904 | 5.3:+6117832 | None:intergenic |
| GGCTGATGGTCATGTTCCGT+GGG | 0.496542 | 5.3:-6116978 | MS.gene09729:CDS |
| TCACCAGAAGGCATACAGAA+TGG | 0.500922 | 5.3:-6120445 | MS.gene09729:CDS |
| CAACTTCAAGAAATTTACAT+TGG | 0.507439 | 5.3:+6120471 | None:intergenic |
| TAACACTTGTTCCAGTGCCA+TGG | 0.516004 | 5.3:+6117831 | None:intergenic |
| TCCTGATCATCCTAGTGCCA+TGG | 0.516526 | 5.3:-6119710 | MS.gene09729:CDS |
| TGATGATGGAGTTGCTAACC+TGG | 0.518682 | 5.3:-6117782 | MS.gene09729:CDS |
| TGGCTGATGGTCATGTTCCG+TGG | 0.518749 | 5.3:-6116979 | MS.gene09729:CDS |
| TTGAAGTTGGATTCACCAGA+AGG | 0.532834 | 5.3:-6120457 | MS.gene09729:CDS |
| GTCGTATGCTCCTGCAGAAG+AGG | 0.539504 | 5.3:-6118392 | MS.gene09729:CDS |
| GTTAATGAAGCCCATGGCAC+TGG | 0.543354 | 5.3:-6117842 | MS.gene09729:intron |
| TTGAAGAATTCAACATGTGC+CGG | 0.544153 | 5.3:-6124943 | None:intergenic |
| AACATGTTCCGCGTAATCCA+TGG | 0.551776 | 5.3:+6119693 | None:intergenic |
| CATGTGCCGGCAAAATTCAC+CGG | 0.559627 | 5.3:-6124930 | MS.gene09729:CDS |
| ATCTATGCAGAATGTTGCTG+AGG | 0.563089 | 5.3:-6120671 | MS.gene09729:intron |
| TGCTAACCTGGAAGATAGAA+GGG | 0.563120 | 5.3:-6117770 | MS.gene09729:intron |
| AGTGAGAAGAATTTGCAGAA+TGG | 0.567688 | 5.3:-6119757 | MS.gene09729:CDS |
| TGAGAAGAATTTGCAGAATG+GGG | 0.569266 | 5.3:-6119755 | MS.gene09729:CDS |
| AGTGAAGATGAAGTTGATGA+TGG | 0.571990 | 5.3:-6117796 | MS.gene09729:CDS |
| TCTTCACCGGAATTATGCAC+CGG | 0.580245 | 5.3:+6124911 | None:intergenic |
| GTAGATATCCTGTCCAGCAA+AGG | 0.581784 | 5.3:-6119805 | MS.gene09729:intron |
| GGCATGGCAATTGAAGCACC+TGG | 0.583904 | 5.3:+6119652 | None:intergenic |
| GACAACCAACCAGAAGAAGA+AGG | 0.585205 | 5.3:+6120627 | None:intergenic |
| TGTGGTAGGGTACTGGCTGA+TGG | 0.593400 | 5.3:-6116992 | MS.gene09729:intron |
| GTTCACTGCTTGGTAATCAT+CGG | 0.601850 | 5.3:+6121307 | None:intergenic |
| TCATGTTCCGTGGGCTTGTG+AGG | 0.609215 | 5.3:-6116969 | MS.gene09729:CDS |
| AATGTGCTTCGAAAGACTGA+AGG | 0.611487 | 5.3:-6122761 | MS.gene09729:intron |
| TCTATGCAGAATGTTGCTGA+GGG | 0.614958 | 5.3:-6120670 | MS.gene09729:intron |
| CAACTTCATCTTCACTATCA+CGG | 0.618030 | 5.3:+6117803 | None:intergenic |
| GAGAAGAATTTGCAGAATGG+GGG | 0.621231 | 5.3:-6119754 | MS.gene09729:CDS |
| TCCGCGTAATCCATGGCACT+AGG | 0.623801 | 5.3:+6119700 | None:intergenic |
| TCCTAGTGCCATGGATTACG+CGG | 0.624999 | 5.3:-6119701 | MS.gene09729:CDS |
| TGCCATGCCCCAATCTTCTG+AGG | 0.626073 | 5.3:-6119638 | MS.gene09729:CDS |
| GTCCTCAGAAGATTGGGGCA+TGG | 0.626934 | 5.3:+6119636 | None:intergenic |
| AACTGACATATTGAGATCAG+AGG | 0.631752 | 5.3:-6121273 | MS.gene09729:intron |
| ACCAGAAGGCATACAGAATG+GGG | 0.639865 | 5.3:-6120443 | MS.gene09729:CDS |
| AAGGTTGCGAGCAGGAATTG+TGG | 0.646352 | 5.3:-6122807 | MS.gene09729:CDS |
| TCTCGAAAGCGTAGACCGAG+AGG | 0.648729 | 5.3:-6120517 | MS.gene09729:CDS |
| GAAAATGCCTCACAAGCCCA+CGG | 0.656006 | 5.3:+6116962 | None:intergenic |
| TCAGGGTCCTCAGAAGATTG+GGG | 0.658194 | 5.3:+6119631 | None:intergenic |
| TTGGAAAACTACCAAAACGG+GGG | 0.690298 | 5.3:-6116474 | MS.gene09729:CDS |
| AGATATCCTGTCCAGCAAAG+GGG | 0.705599 | 5.3:-6119803 | MS.gene09729:CDS |
| ATTGGAAAACTACCAAAACG+GGG | 0.706807 | 5.3:-6116475 | MS.gene09729:CDS |
| GGGGTTCTACAGAAAGACAA+CGG | 0.726373 | 5.3:-6120424 | MS.gene09729:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6116452 | 6124951 | 6116452 | ID=MS.gene09729 |
| chr5.3 | mRNA | 6116452 | 6124951 | 6116452 | ID=MS.gene09729.t1;Parent=MS.gene09729 |
| chr5.3 | exon | 6124826 | 6124951 | 6124826 | ID=MS.gene09729.t1.exon1;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6124826 | 6124951 | 6124826 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6124179 | 6124234 | 6124179 | ID=MS.gene09729.t1.exon2;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6124179 | 6124234 | 6124179 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6122762 | 6122826 | 6122762 | ID=MS.gene09729.t1.exon3;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6122762 | 6122826 | 6122762 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6122323 | 6122375 | 6122323 | ID=MS.gene09729.t1.exon4;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6122323 | 6122375 | 6122323 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6121417 | 6121470 | 6121417 | ID=MS.gene09729.t1.exon5;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6121417 | 6121470 | 6121417 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6121274 | 6121333 | 6121274 | ID=MS.gene09729.t1.exon6;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6121274 | 6121333 | 6121274 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6120637 | 6120683 | 6120637 | ID=MS.gene09729.t1.exon7;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6120637 | 6120683 | 6120637 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6120425 | 6120546 | 6120425 | ID=MS.gene09729.t1.exon8;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6120425 | 6120546 | 6120425 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6119547 | 6119823 | 6119547 | ID=MS.gene09729.t1.exon9;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6119547 | 6119823 | 6119547 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6118365 | 6118413 | 6118365 | ID=MS.gene09729.t1.exon10;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6118365 | 6118413 | 6118365 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6117771 | 6117854 | 6117771 | ID=MS.gene09729.t1.exon11;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6117771 | 6117854 | 6117771 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6117143 | 6117222 | 6117143 | ID=MS.gene09729.t1.exon12;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6117143 | 6117222 | 6117143 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6116917 | 6117006 | 6116917 | ID=MS.gene09729.t1.exon13;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6116917 | 6117006 | 6116917 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| chr5.3 | exon | 6116452 | 6116575 | 6116452 | ID=MS.gene09729.t1.exon14;Parent=MS.gene09729.t1 |
| chr5.3 | CDS | 6116452 | 6116575 | 6116452 | ID=cds.MS.gene09729.t1;Parent=MS.gene09729.t1 |
| Gene Sequence |
| Protein sequence |
