Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20342.t1 | RHN81205.1 | 98.1 | 52 | 1 | 0 | 1 | 52 | 680 | 731 | 3.00E-21 | 110.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20342.t1 | A0A396JYX6 | 98.1 | 52 | 1 | 0 | 1 | 52 | 680 | 731 | 2.2e-21 | 110.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052519 | MS.gene20342 | 0.834958 | 2.22E-56 | -1.69E-46 |
| MS.gene057693 | MS.gene20342 | 0.800465 | 1.42E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCAGAACAGGAAATATTC+TGG | 0.287164 | 1.1:-65826997 | None:intergenic |
| TTTATGATTTGAGGACAATA+TGG | 0.324306 | 1.1:-65826974 | None:intergenic |
| AGGATGGTTGATCATTCTTA+TGG | 0.354581 | 1.1:-65827122 | None:intergenic |
| TCATCAGTTTCTTGAAGAAT+AGG | 0.369535 | 1.1:-65826834 | None:intergenic |
| ATATTCTGGTTTATGATTTG+AGG | 0.444118 | 1.1:-65826983 | None:intergenic |
| TACCTGTAAAAGTTCAGAAC+AGG | 0.463097 | 1.1:-65827008 | None:intergenic |
| GGTCTATGTCTTTACGAGGA+TGG | 0.493066 | 1.1:-65827138 | None:intergenic |
| ACATCTTAGTGATGTAAACC+AGG | 0.599828 | 1.1:+65826863 | MS.gene20342:CDS |
| GGTTGGTCTATGTCTTTACG+AGG | 0.660233 | 1.1:-65827142 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATTTGATTATTTCTTG+TGG | + | chr1.1:65827030-65827049 | MS.gene20342:intron | 15.0% |
| !! | TTATTTGAACATTTACATTG+TGG | + | chr1.1:65826919-65826938 | MS.gene20342:intron | 20.0% |
| ! | TTTATGATTTGAGGACAATA+TGG | - | chr1.1:65826977-65826996 | None:intergenic | 25.0% |
| !!! | AAATAAAAATAAAGAGCACC+AGG | - | chr1.1:65826905-65826924 | None:intergenic | 25.0% |
| !!! | ATATTCTGGTTTATGATTTG+AGG | - | chr1.1:65826986-65827005 | None:intergenic | 25.0% |
| TCATCAGTTTCTTGAAGAAT+AGG | - | chr1.1:65826837-65826856 | None:intergenic | 30.0% | |
| TGATAAAACATCTGTTTCGT+AGG | + | chr1.1:65826949-65826968 | MS.gene20342:intron | 30.0% | |
| ! | GATTATTTCTTGTGGTAACA+TGG | + | chr1.1:65827038-65827057 | MS.gene20342:intron | 30.0% |
| ACATCTTAGTGATGTAAACC+AGG | + | chr1.1:65826863-65826882 | MS.gene20342:CDS | 35.0% | |
| AGGATGGTTGATCATTCTTA+TGG | - | chr1.1:65827125-65827144 | None:intergenic | 35.0% | |
| GGTGATGAAAATATAGAACC+TGG | + | chr1.1:65826884-65826903 | MS.gene20342:intron | 35.0% | |
| GTTCAGAACAGGAAATATTC+TGG | - | chr1.1:65827000-65827019 | None:intergenic | 35.0% | |
| TACCTGTAAAAGTTCAGAAC+AGG | - | chr1.1:65827011-65827030 | None:intergenic | 35.0% | |
| ! | GGTTCTATATTTTCATCACC+TGG | - | chr1.1:65826884-65826903 | None:intergenic | 35.0% |
| !! | TTCCTGTTCTGAACTTTTAC+AGG | + | chr1.1:65827006-65827025 | MS.gene20342:CDS | 35.0% |
| AATGATCAACCATCCTCGTA+AGG | + | chr1.1:65827129-65827148 | MS.gene20342:CDS | 40.0% | |
| TGCCTGCAAAAAGAACACAT+GGG | - | chr1.1:65827097-65827116 | None:intergenic | 40.0% | |
| GTGCCTGCAAAAAGAACACA+TGG | - | chr1.1:65827098-65827117 | None:intergenic | 45.0% | |
| ! | TGCCCATGTGTTCTTTTTGC+AGG | + | chr1.1:65827092-65827111 | MS.gene20342:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 65826819 | 65827149 | 65826819 | ID=MS.gene20342 |
| chr1.1 | mRNA | 65826819 | 65827149 | 65826819 | ID=MS.gene20342.t1;Parent=MS.gene20342 |
| chr1.1 | exon | 65826819 | 65826884 | 65826819 | ID=MS.gene20342.t1.exon1;Parent=MS.gene20342.t1 |
| chr1.1 | CDS | 65826819 | 65826884 | 65826819 | ID=cds.MS.gene20342.t1;Parent=MS.gene20342.t1 |
| chr1.1 | exon | 65826971 | 65827027 | 65826971 | ID=MS.gene20342.t1.exon2;Parent=MS.gene20342.t1 |
| chr1.1 | CDS | 65826971 | 65827027 | 65826971 | ID=cds.MS.gene20342.t1;Parent=MS.gene20342.t1 |
| chr1.1 | exon | 65827114 | 65827149 | 65827114 | ID=MS.gene20342.t1.exon3;Parent=MS.gene20342.t1 |
| chr1.1 | CDS | 65827114 | 65827149 | 65827114 | ID=cds.MS.gene20342.t1;Parent=MS.gene20342.t1 |
| Gene Sequence |
| Protein sequence |
