Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21006.t1 | XP_013469076.1 | 98.3 | 484 | 8 | 0 | 1 | 484 | 1 | 484 | 1.40E-282 | 981.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21006.t1 | F4HZG9 | 68.7 | 469 | 144 | 2 | 1 | 469 | 1 | 466 | 2.8e-194 | 679.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21006.t1 | A0A072VM73 | 98.3 | 484 | 8 | 0 | 1 | 484 | 1 | 484 | 1.0e-282 | 981.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene21006 | MS.gene02843 | PPI |
| MS.gene21006 | MS.gene048313 | PPI |
| MS.gene21006 | MS.gene006974 | PPI |
| MS.gene21006 | MS.gene003186 | PPI |
| MS.gene21006 | MS.gene02160 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21006.t1 | MTR_1g084190 | 98.347 | 484 | 8 | 0 | 1 | 484 | 1 | 484 | 0.0 | 997 |
| MS.gene21006.t1 | MTR_8g101730 | 26.371 | 474 | 286 | 16 | 51 | 464 | 8 | 478 | 3.83e-37 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21006.t1 | AT1G09300 | 68.657 | 469 | 144 | 2 | 1 | 469 | 1 | 466 | 0.0 | 691 |
| MS.gene21006.t1 | AT1G09300 | 70.794 | 428 | 123 | 1 | 42 | 469 | 10 | 435 | 0.0 | 653 |
| MS.gene21006.t1 | AT4G29490 | 25.176 | 425 | 259 | 14 | 93 | 464 | 59 | 477 | 3.05e-37 | 143 |
Find 127 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTAGCTCTCAAGTTATTT+TGG | 0.237616 | 1.3:-59961206 | None:intergenic |
| CTCACATAAGGTTTGGCTTC+TGG | 0.259772 | 1.3:-59953199 | None:intergenic |
| TGAATTTGCTTCACTTCTTT+CGG | 0.281191 | 1.3:-59961141 | None:intergenic |
| ACCAATGTCATGCCCTAAAA+TGG | 0.283309 | 1.3:-59953161 | None:intergenic |
| GAGGAACCCCTTACCATATC+TGG | 0.297990 | 1.3:-59954336 | None:intergenic |
| TAGAAGTTGGGTTCAGCTTA+TGG | 0.298215 | 1.3:-59957276 | None:intergenic |
| ACATCTGTCATCATCTTTAC+TGG | 0.311255 | 1.3:-59953067 | None:intergenic |
| AATTAGCATTTGCAGGTTCT+AGG | 0.321480 | 1.3:+59951972 | MS.gene21006:intron |
| GCCGATTACTCATATATTAC+AGG | 0.336669 | 1.3:+59953115 | MS.gene21006:CDS |
| ACAACTTCAGCCATGGAATA+GGG | 0.337029 | 1.3:+59961175 | MS.gene21006:CDS |
| ACCTCGTTCAACTTTCTCAT+TGG | 0.343552 | 1.3:-59954089 | None:intergenic |
| TATTCAGATCCTTCCAGATA+TGG | 0.348584 | 1.3:+59954323 | MS.gene21006:intron |
| GCATCTTGTCGAAATGTATA+AGG | 0.350875 | 1.3:-59953094 | None:intergenic |
| ATTGATCCAGGTCACTATCT+AGG | 0.354401 | 1.3:+59957423 | MS.gene21006:intron |
| GACTGCTACATCAGCATATA+CGG | 0.360739 | 1.3:+59954380 | MS.gene21006:CDS |
| AATTTGTCTAGGATGTGATT+TGG | 0.363924 | 1.3:+59954006 | MS.gene21006:intron |
| CTTTCGGAATTGATGCTGTT+AGG | 0.367090 | 1.3:-59961125 | None:intergenic |
| GAAGTGTTATACATTATTCT+AGG | 0.368162 | 1.3:+59955035 | MS.gene21006:CDS |
| ATCTGCATCAATTGCTTGTC+AGG | 0.376334 | 1.3:+59954515 | MS.gene21006:CDS |
| ATGATAGCCAAACTCTTCTC+TGG | 0.383373 | 1.3:-59953034 | None:intergenic |
| GAGATTCTCATTACAGAAAC+AGG | 0.389989 | 1.3:+59960014 | MS.gene21006:CDS |
| CTGTTTGTGTGAAAATGTTT+TGG | 0.397576 | 1.3:-59951846 | None:intergenic |
| TTCAATCCCGTGGTTGGTGG+TGG | 0.398757 | 1.3:+59955004 | MS.gene21006:CDS |
| CATGTTTGGCAGATTAAAGA+TGG | 0.406377 | 1.3:+59955129 | MS.gene21006:intron |
| AAGCTGAACCCAACTTCTAT+AGG | 0.407794 | 1.3:+59957280 | MS.gene21006:CDS |
| TCTGGACAATTAAAAGAAGA+TGG | 0.413497 | 1.3:-59958541 | None:intergenic |
| AACAACTTCAGCCATGGAAT+AGG | 0.413497 | 1.3:+59961174 | MS.gene21006:CDS |
| GTGGTTGGTGGTGGGCCTAA+TGG | 0.419819 | 1.3:+59955013 | MS.gene21006:CDS |
| ACAGTAAGCTAGTTTCTTGA+AGG | 0.421447 | 1.3:-59954412 | None:intergenic |
| GCCTGTAATATATGAGTAAT+CGG | 0.422629 | 1.3:-59953116 | None:intergenic |
| AGTACCTGCGGGTGAGATGC+AGG | 0.425788 | 1.3:-59952047 | None:intergenic |
| GCAGCTAGCATACCTTCAAA+AGG | 0.428825 | 1.3:-59954728 | None:intergenic |
| GCATTGTAGCTTCTAAAAGA+TGG | 0.435409 | 1.3:+59952944 | MS.gene21006:intron |
| TATCAGACTCACATAAGGTT+TGG | 0.437944 | 1.3:-59953206 | None:intergenic |
| GCTTCTCCCAGAGAAGAGTT+TGG | 0.441988 | 1.3:+59953027 | MS.gene21006:CDS |
| CACAAGGTTTCTGTTGATGT+TGG | 0.444626 | 1.3:+59952014 | MS.gene21006:CDS |
| CATTACAGAAACAGGTTATG+AGG | 0.448108 | 1.3:+59960022 | MS.gene21006:CDS |
| ATGAGAGGTGCACAGCGAAT+GGG | 0.448546 | 1.3:+59954773 | MS.gene21006:CDS |
| CATCTGTCATCATCTTTACT+GGG | 0.449826 | 1.3:-59953066 | None:intergenic |
| AACTTTAGTAAGTACCTCTC+TGG | 0.452530 | 1.3:-59958559 | None:intergenic |
| GGCTATCAACCCCTATTCCA+TGG | 0.458885 | 1.3:-59961185 | None:intergenic |
| CATTGTAGCTTCTAAAAGAT+GGG | 0.460019 | 1.3:+59952945 | MS.gene21006:intron |
| CCTAAAATGGCCACACCACC+AGG | 0.460454 | 1.3:-59953148 | None:intergenic |
| TGGTAGCTTCTCTTCTGCCC+AGG | 0.468113 | 1.3:+59955227 | MS.gene21006:CDS |
| GGTACCAAGGCATTGGGATA+AGG | 0.470535 | 1.3:+59959982 | MS.gene21006:intron |
| CCGAATGGTTGTGTATTTGT+CGG | 0.470570 | 1.3:-59956226 | None:intergenic |
| TGCAGCATTGAATACATTCA+AGG | 0.471710 | 1.3:+59954052 | MS.gene21006:CDS |
| ACCAGGCTACCTATAGAAGT+TGG | 0.472649 | 1.3:-59957289 | None:intergenic |
| TAATGCAGGTACCAAGGCAT+TGG | 0.480015 | 1.3:+59959975 | MS.gene21006:intron |
| TTCATCAGCTTGAGCTCTGA+AGG | 0.481437 | 1.3:-59954489 | None:intergenic |
| TGTGTGGAACTTTGTAAGCC+TGG | 0.488945 | 1.3:+59956194 | MS.gene21006:CDS |
| TTTGACTGTCCTCTGAAGCC+AGG | 0.489111 | 1.3:+59957477 | MS.gene21006:CDS |
| AGATCCTTCCAGATATGGTA+AGG | 0.492996 | 1.3:+59954328 | MS.gene21006:intron |
| GTCTAGGATGTGATTTGGCA+AGG | 0.493497 | 1.3:+59954011 | MS.gene21006:intron |
| AACATTGTGATACAATTTCG+AGG | 0.498748 | 1.3:-59954355 | None:intergenic |
| TGGCCACACCACCAGGTTGT+TGG | 0.502948 | 1.3:-59953141 | None:intergenic |
| AATGAGAGGTGCACAGCGAA+TGG | 0.502962 | 1.3:+59954772 | MS.gene21006:CDS |
| TAATGTATAACACTTCCATT+AGG | 0.503380 | 1.3:-59955028 | None:intergenic |
| TTCAGAGCTCAAGCTGATGA+AGG | 0.504671 | 1.3:+59954491 | MS.gene21006:CDS |
| ATTTGGCAAGGGCATGTAGC+AGG | 0.507631 | 1.3:+59954023 | MS.gene21006:CDS |
| TCAGCGATCTCACGCGTACC+TGG | 0.508919 | 1.3:+59955196 | MS.gene21006:CDS |
| CAGAGAGGACTCAAGGAGGT+TGG | 0.513664 | 1.3:+59957223 | MS.gene21006:CDS |
| AGATTCAATCCCGTGGTTGG+TGG | 0.514269 | 1.3:+59955001 | MS.gene21006:intron |
| ATCAACAGAAACCTTGTGAC+TGG | 0.514653 | 1.3:-59952009 | None:intergenic |
| AATGCAGGTACCAAGGCATT+GGG | 0.515702 | 1.3:+59959976 | MS.gene21006:intron |
| CTGGACAATTAAAAGAAGAT+GGG | 0.518907 | 1.3:-59958540 | None:intergenic |
| TACATCAGCATATACGGAAC+TGG | 0.519360 | 1.3:+59954386 | MS.gene21006:CDS |
| TGAAGGTATGCTAGCTGCAA+AGG | 0.521850 | 1.3:+59954733 | MS.gene21006:CDS |
| GAAGCTACCACAAGGTGGCC+AGG | 0.525969 | 1.3:-59955214 | None:intergenic |
| CCAGGCTACCTATAGAAGTT+GGG | 0.533663 | 1.3:-59957288 | None:intergenic |
| CTTACAACACCTGGCTTCAG+AGG | 0.541034 | 1.3:-59957486 | None:intergenic |
| CAGCTAGCATACCTTCAAAA+GGG | 0.544862 | 1.3:-59954727 | None:intergenic |
| TGCACATGGACAGTATCAGC+TGG | 0.550753 | 1.3:-59961229 | None:intergenic |
| TTTATACCCATCAGCTGCGA+TGG | 0.553980 | 1.3:+59954457 | MS.gene21006:CDS |
| TATTCCTCACTAGATATGCC+TGG | 0.555430 | 1.3:-59952977 | None:intergenic |
| TGCGGGTGAGATGCAGGGGT+TGG | 0.558374 | 1.3:-59952041 | None:intergenic |
| TCTAGGATGTGATTTGGCAA+GGG | 0.563003 | 1.3:+59954012 | MS.gene21006:intron |
| AGGCATTGGGATAAGGATTG+AGG | 0.564277 | 1.3:+59959989 | MS.gene21006:CDS |
| GAAGATGGGATGTACACTCC+TGG | 0.565328 | 1.3:-59958526 | None:intergenic |
| CCGACAAATACACAACCATT+CGG | 0.567790 | 1.3:+59956226 | MS.gene21006:CDS |
| AGTTGGGTTCAGCTTATGGT+AGG | 0.568189 | 1.3:-59957272 | None:intergenic |
| ATGGCTGAAGTTGTTTAGCA+AGG | 0.569030 | 1.3:-59961166 | None:intergenic |
| GGGAAAGCACTTACAACACC+TGG | 0.570813 | 1.3:-59957495 | None:intergenic |
| AACCCCTGCATCTCACCCGC+AGG | 0.573533 | 1.3:+59952043 | MS.gene21006:CDS |
| ATTAGGCCCACCACCAACCA+CGG | 0.575070 | 1.3:-59955011 | None:intergenic |
| CAATCCTTATCCCAATGCCT+TGG | 0.576534 | 1.3:-59959986 | None:intergenic |
| TTGCTAAACAACTTCAGCCA+TGG | 0.577584 | 1.3:+59961168 | MS.gene21006:CDS |
| TCAATCCCGTGGTTGGTGGT+GGG | 0.577700 | 1.3:+59955005 | MS.gene21006:CDS |
| GTACCTGCGGGTGAGATGCA+GGG | 0.580749 | 1.3:-59952046 | None:intergenic |
| CAACTTCAGCCATGGAATAG+GGG | 0.581494 | 1.3:+59961176 | MS.gene21006:CDS |
| TACCTGCGGGTGAGATGCAG+GGG | 0.581809 | 1.3:-59952045 | None:intergenic |
| GACATTGGATGTGAGTTACA+TGG | 0.582230 | 1.3:+59955168 | MS.gene21006:CDS |
| TGATAGCCAAACTCTTCTCT+GGG | 0.583126 | 1.3:-59953033 | None:intergenic |
| TCTCATTGGATACGCCTCGT+TGG | 0.585869 | 1.3:-59954075 | None:intergenic |
| GATCCTTCCAGATATGGTAA+GGG | 0.586115 | 1.3:+59954329 | MS.gene21006:intron |
| AAAGATGGGGAGATCACACC+AGG | 0.587344 | 1.3:+59952959 | MS.gene21006:CDS |
| ATCAGATTCAATCCCGTGGT+TGG | 0.587531 | 1.3:+59954998 | MS.gene21006:intron |
| TTAGGCCCACCACCAACCAC+GGG | 0.589559 | 1.3:-59955010 | None:intergenic |
| TGAGAGGTGCACAGCGAATG+GGG | 0.590182 | 1.3:+59954774 | MS.gene21006:CDS |
| AGCTTACTGTAACAACGTAA+AGG | 0.590674 | 1.3:+59954425 | MS.gene21006:CDS |
| CTGCCAACAACCTGGTGGTG+TGG | 0.593769 | 1.3:+59953138 | MS.gene21006:CDS |
| TCGTTCTTATTCCAGTCACA+AGG | 0.594752 | 1.3:+59951998 | MS.gene21006:CDS |
| ATTACAGGCTGCCAACAACC+TGG | 0.596169 | 1.3:+59953130 | MS.gene21006:CDS |
| ACGCGTACCTGGCCACCTTG+TGG | 0.599158 | 1.3:+59955207 | MS.gene21006:CDS |
| CAGGTAATAACTATTGAACC+AGG | 0.600635 | 1.3:+59958508 | MS.gene21006:intron |
| TCCAGGTCACTATCTAGGAA+TGG | 0.601316 | 1.3:+59957428 | MS.gene21006:intron |
| AGAAACTAACAAGCATTGTG+TGG | 0.607256 | 1.3:+59956178 | MS.gene21006:CDS |
| AATGCTGCAGAGAGGACTCA+AGG | 0.621857 | 1.3:+59957216 | MS.gene21006:CDS |
| TTATTCTAGGAACGATCAGA+AGG | 0.629437 | 1.3:+59955048 | MS.gene21006:CDS |
| ATCCTTCCAGATATGGTAAG+GGG | 0.630528 | 1.3:+59954330 | MS.gene21006:CDS |
| CTGTATCAGATTCAATCCCG+TGG | 0.633552 | 1.3:+59954994 | MS.gene21006:intron |
| ATTGTAGCTTCTAAAAGATG+GGG | 0.641791 | 1.3:+59952946 | MS.gene21006:intron |
| ATTTGTCGGATACTAGCACC+AGG | 0.642966 | 1.3:-59956212 | None:intergenic |
| GATCTCTAATGCAGGTACCA+AGG | 0.651356 | 1.3:+59959969 | MS.gene21006:intron |
| AACCCCTTACCATATCTGGA+AGG | 0.655197 | 1.3:-59954332 | None:intergenic |
| ATCAGCATATACGGAACTGG+AGG | 0.655539 | 1.3:+59954389 | MS.gene21006:CDS |
| CACACCAGGCATATCTAGTG+AGG | 0.673963 | 1.3:+59952973 | MS.gene21006:CDS |
| ATCTGTCATCATCTTTACTG+GGG | 0.686632 | 1.3:-59953065 | None:intergenic |
| ACATTGGATGTGAGTTACAT+GGG | 0.693361 | 1.3:+59955169 | MS.gene21006:CDS |
| GAATACGAATGCAAAATGAG+AGG | 0.696219 | 1.3:+59954758 | MS.gene21006:CDS |
| GCAGAAGAGAAGCTACCACA+AGG | 0.709712 | 1.3:-59955222 | None:intergenic |
| CAGGTTGAAATGCTGCAGAG+AGG | 0.718249 | 1.3:+59957208 | MS.gene21006:intron |
| GCTGCAGAGAGGACTCAAGG+AGG | 0.732510 | 1.3:+59957219 | MS.gene21006:CDS |
| GAAGAGAAGCTACCACAAGG+TGG | 0.733869 | 1.3:-59955219 | None:intergenic |
| TCCAATGAGAAAGTTGAACG+AGG | 0.743293 | 1.3:+59954088 | MS.gene21006:CDS |
| ACAGGCTGCCAACAACCTGG+TGG | 0.744058 | 1.3:+59953133 | MS.gene21006:CDS |
| GAATACATTCAAGGCCAACG+AGG | 0.787952 | 1.3:+59954061 | MS.gene21006:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 59951821 | 59961236 | 59951821 | ID=MS.gene21006 |
| chr1.3 | mRNA | 59951821 | 59961236 | 59951821 | ID=MS.gene21006.t1;Parent=MS.gene21006 |
| chr1.3 | exon | 59951821 | 59951868 | 59951821 | ID=MS.gene21006.t1.exon1;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59951821 | 59951868 | 59951821 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59951987 | 59952064 | 59951987 | ID=MS.gene21006.t1.exon2;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59951987 | 59952064 | 59951987 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59952953 | 59953216 | 59952953 | ID=MS.gene21006.t1.exon3;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59952953 | 59953216 | 59952953 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59954017 | 59954109 | 59954017 | ID=MS.gene21006.t1.exon4;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59954017 | 59954109 | 59954017 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59954330 | 59954536 | 59954330 | ID=MS.gene21006.t1.exon5;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59954330 | 59954536 | 59954330 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59954692 | 59954795 | 59954692 | ID=MS.gene21006.t1.exon6;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59954692 | 59954795 | 59954692 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59955003 | 59955069 | 59955003 | ID=MS.gene21006.t1.exon7;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59955003 | 59955069 | 59955003 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59955141 | 59955248 | 59955141 | ID=MS.gene21006.t1.exon8;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59955141 | 59955248 | 59955141 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59956155 | 59956247 | 59956155 | ID=MS.gene21006.t1.exon9;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59956155 | 59956247 | 59956155 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59957211 | 59957301 | 59957211 | ID=MS.gene21006.t1.exon10;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59957211 | 59957301 | 59957211 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59957433 | 59957503 | 59957433 | ID=MS.gene21006.t1.exon11;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59957433 | 59957503 | 59957433 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59958511 | 59958566 | 59958511 | ID=MS.gene21006.t1.exon12;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59958511 | 59958566 | 59958511 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59959983 | 59960043 | 59959983 | ID=MS.gene21006.t1.exon13;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59959983 | 59960043 | 59959983 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| chr1.3 | exon | 59961123 | 59961236 | 59961123 | ID=MS.gene21006.t1.exon14;Parent=MS.gene21006.t1 |
| chr1.3 | CDS | 59961123 | 59961236 | 59961123 | ID=cds.MS.gene21006.t1;Parent=MS.gene21006.t1 |
| Gene Sequence |
| Protein sequence |
