Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21036.t1 | XP_013469076.1 | 98 | 449 | 9 | 0 | 1 | 449 | 1 | 449 | 1.10E-261 | 912.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21036.t1 | F4HZG9 | 68.8 | 449 | 137 | 2 | 1 | 449 | 1 | 446 | 3.2e-185 | 649.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21036.t1 | A0A072VM73 | 98.0 | 449 | 9 | 0 | 1 | 449 | 1 | 449 | 7.8e-262 | 912.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene02843 | MS.gene21036 | PPI |
| MS.gene048313 | MS.gene21036 | PPI |
| MS.gene21036 | MS.gene003186 | PPI |
| MS.gene006974 | MS.gene21036 | PPI |
| MS.gene21036 | MS.gene02160 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21036.t1 | MTR_1g084190 | 97.561 | 451 | 11 | 0 | 1 | 451 | 1 | 451 | 0.0 | 925 |
| MS.gene21036.t1 | MTR_8g101730 | 27.033 | 455 | 270 | 15 | 51 | 445 | 8 | 460 | 1.17e-35 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21036.t1 | AT1G09300 | 68.820 | 449 | 137 | 2 | 1 | 449 | 1 | 446 | 0.0 | 660 |
| MS.gene21036.t1 | AT1G09300 | 71.078 | 408 | 116 | 1 | 42 | 449 | 10 | 415 | 0.0 | 623 |
| MS.gene21036.t1 | AT4G29490 | 25.862 | 406 | 243 | 14 | 93 | 445 | 59 | 459 | 4.80e-35 | 137 |
Find 125 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCACATAAGGTTTGGCTTC+TGG | 0.259772 | 1.4:+68825031 | None:intergenic |
| TGAATTTGCTTCACTTCTTT+CGG | 0.281191 | 1.4:+68817281 | None:intergenic |
| ACCAATGTCATGCCCTAAAA+TGG | 0.283309 | 1.4:+68825069 | None:intergenic |
| TAGAAGTTGGGTTCAGCTTA+TGG | 0.298215 | 1.4:+68821147 | None:intergenic |
| GAGGAACCCCTTATCATATC+TGG | 0.302925 | 1.4:+68823888 | None:intergenic |
| ACATCTGTCATCATCTTTAC+TGG | 0.311255 | 1.4:+68825163 | None:intergenic |
| AATTAGCATTTGCAGGTTCT+AGG | 0.321480 | 1.4:-68826248 | MS.gene21036:intron |
| GCCGATTACTCATATATTAC+AGG | 0.336669 | 1.4:-68825115 | MS.gene21036:CDS |
| ACCTCGTTCAACTTTCTCAT+TGG | 0.343552 | 1.4:+68824135 | None:intergenic |
| GCATCTTGTCGAAATGTATA+AGG | 0.350875 | 1.4:+68825136 | None:intergenic |
| ATTGATCCAGGTCACTATCT+AGG | 0.354401 | 1.4:-68821000 | MS.gene21036:intron |
| AATTTGTCTAGGATGTGATT+TGG | 0.363924 | 1.4:-68824218 | MS.gene21036:intron |
| CTTTCGGAATTGATGCTGTT+AGG | 0.367090 | 1.4:+68817297 | None:intergenic |
| GAAGTGTTATACATTATTCT+AGG | 0.368162 | 1.4:-68823189 | MS.gene21036:CDS |
| ATCTGCATCAATTGCTTGTC+AGG | 0.376334 | 1.4:-68823709 | MS.gene21036:intron |
| ATGATAGCCAAACTCTTCTC+TGG | 0.383373 | 1.4:+68825196 | None:intergenic |
| GAGATTCTCATTACAGAAAC+AGG | 0.389989 | 1.4:-68818408 | MS.gene21036:CDS |
| CTGTTTGTGTGAAAATGTTT+TGG | 0.397576 | 1.4:+68826374 | None:intergenic |
| TTCAATCCCGTGGTTGGTGG+TGG | 0.398757 | 1.4:-68823220 | MS.gene21036:CDS |
| CATGTTTGGCAGATTAAAGA+TGG | 0.406377 | 1.4:-68823095 | MS.gene21036:intron |
| AAGCTGAACCCAACTTCTAT+AGG | 0.407794 | 1.4:-68821143 | MS.gene21036:intron |
| ATGAGAGGTGCGCAGCGAAT+GGG | 0.410718 | 1.4:-68823451 | MS.gene21036:CDS |
| AGTTTATTTGTGACAGATAG+AGG | 0.419351 | 1.4:-68818339 | MS.gene21036:intron |
| GTGGTTGGTGGTGGGCCTAA+TGG | 0.419819 | 1.4:-68823211 | MS.gene21036:CDS |
| ACAGTAAGCTAGTTTCTTGA+AGG | 0.421447 | 1.4:+68823812 | None:intergenic |
| AACAACTTCAGCCATGGAAT+GGG | 0.421682 | 1.4:-68817248 | MS.gene21036:CDS |
| GCCTGTAATATATGAGTAAT+CGG | 0.422629 | 1.4:+68825114 | None:intergenic |
| GTTGGAATATTGAAAGATGT+TGG | 0.424223 | 1.4:-68821182 | MS.gene21036:CDS |
| AGTACCTGCGGGTGAGATGC+AGG | 0.425788 | 1.4:+68826173 | None:intergenic |
| GCAGCTAGCATACCTTCAAA+AGG | 0.428825 | 1.4:+68823496 | None:intergenic |
| GATCCTTCCAGATATGATAA+GGG | 0.429047 | 1.4:-68823895 | MS.gene21036:CDS |
| GCATTGTAGCTTCTAAAAGA+TGG | 0.435409 | 1.4:-68825286 | MS.gene21036:intron |
| TATCAGACTCACATAAGGTT+TGG | 0.437944 | 1.4:+68825024 | None:intergenic |
| AGATCCTTCCAGATATGATA+AGG | 0.439022 | 1.4:-68823896 | MS.gene21036:CDS |
| GCTTCTCCCAGAGAAGAGTT+TGG | 0.441988 | 1.4:-68825203 | MS.gene21036:CDS |
| CATTACAGAAACAGGTTATG+AGG | 0.448108 | 1.4:-68818400 | MS.gene21036:CDS |
| CATCTGTCATCATCTTTACT+GGG | 0.449826 | 1.4:+68825164 | None:intergenic |
| AACTTTAGTAAGTACCTCTC+TGG | 0.452530 | 1.4:+68819864 | None:intergenic |
| CATTGTAGCTTCTAAAAGAT+GGG | 0.460019 | 1.4:-68825285 | MS.gene21036:intron |
| CCTAAAATGGCCACACCACC+AGG | 0.460454 | 1.4:+68825082 | None:intergenic |
| TGGTAGCTTCTCTTCTGCCC+AGG | 0.468113 | 1.4:-68822997 | MS.gene21036:intron |
| GGTACCAAGGCATTGGGATA+AGG | 0.470535 | 1.4:-68818440 | MS.gene21036:CDS |
| CCGAATGGTTGTGTATTTGT+CGG | 0.470570 | 1.4:+68821997 | None:intergenic |
| TGCAGCATTGAATACATTCA+AGG | 0.471710 | 1.4:-68824172 | MS.gene21036:CDS |
| CACAAGGTTTCCGTTGATGT+TGG | 0.475869 | 1.4:-68826206 | MS.gene21036:CDS |
| TAATGCAGGTACCAAGGCAT+TGG | 0.480015 | 1.4:-68818447 | MS.gene21036:intron |
| TTCATCAGCTTGAGCTCTGA+AGG | 0.481437 | 1.4:+68823735 | None:intergenic |
| TGTGTGGAACTTTGTAAGCC+TGG | 0.488945 | 1.4:-68822029 | MS.gene21036:CDS |
| TTTGACTGTCCTCTGAAGCC+AGG | 0.489111 | 1.4:-68820946 | MS.gene21036:CDS |
| ACCAGGCTACCTATAGAAGT+TGG | 0.492131 | 1.4:+68821134 | None:intergenic |
| GTCTAGGATGTGATTTGGCA+AGG | 0.493497 | 1.4:-68824213 | MS.gene21036:intron |
| AAACAACTTCAGCCATGGAA+TGG | 0.496327 | 1.4:-68817249 | MS.gene21036:CDS |
| AACATTGTGATACAATTTCG+AGG | 0.498748 | 1.4:+68823869 | None:intergenic |
| AATGAGAGGTGCGCAGCGAA+TGG | 0.501064 | 1.4:-68823452 | MS.gene21036:CDS |
| TGGCCACACCACCAGGTTGT+TGG | 0.502948 | 1.4:+68825089 | None:intergenic |
| TAATGTATAACACTTCCATT+AGG | 0.503380 | 1.4:+68823196 | None:intergenic |
| TTCAGAGCTCAAGCTGATGA+AGG | 0.504671 | 1.4:-68823733 | MS.gene21036:CDS |
| ATTTGGCAAGGGCATGTAGC+AGG | 0.507631 | 1.4:-68824201 | MS.gene21036:CDS |
| TCAGCGATCTCACGCGTACC+TGG | 0.508919 | 1.4:-68823028 | MS.gene21036:CDS |
| ATCTATCTGTTTATACCCAT+CGG | 0.510938 | 1.4:-68823776 | MS.gene21036:CDS |
| TACATCAGCATATACTGAAC+TGG | 0.512568 | 1.4:-68823838 | MS.gene21036:CDS |
| CAGAAAGGACTCAAGGAGGT+TGG | 0.513664 | 1.4:-68821200 | MS.gene21036:CDS |
| AGATTCAATCCCGTGGTTGG+TGG | 0.514269 | 1.4:-68823223 | MS.gene21036:CDS |
| AATGCAGGTACCAAGGCATT+GGG | 0.515702 | 1.4:-68818446 | MS.gene21036:intron |
| TGAAGGTATGCTAGCTGCAA+AGG | 0.521850 | 1.4:-68823491 | MS.gene21036:CDS |
| GAAGCTACCACAAGGTGGCC+AGG | 0.525969 | 1.4:+68823010 | None:intergenic |
| TTTATACCCATCGGCTGCGA+TGG | 0.528638 | 1.4:-68823767 | MS.gene21036:CDS |
| ATCAACGGAAACCTTGTGAC+TGG | 0.532664 | 1.4:+68826211 | None:intergenic |
| CCAGGCTACCTATAGAAGTT+GGG | 0.533663 | 1.4:+68821135 | None:intergenic |
| CTTACAACACCTGGCTTCAG+AGG | 0.541034 | 1.4:+68820937 | None:intergenic |
| CAGCTAGCATACCTTCAAAA+GGG | 0.544862 | 1.4:+68823497 | None:intergenic |
| AAAGATGGGATGTACACTCC+TGG | 0.554857 | 1.4:+68819897 | None:intergenic |
| TATTCCTCACTAGATATGCC+TGG | 0.555430 | 1.4:+68825253 | None:intergenic |
| TGCGGGTGAGATGCAGGGGT+TGG | 0.558374 | 1.4:+68826179 | None:intergenic |
| TCTAGGATGTGATTTGGCAA+GGG | 0.563003 | 1.4:-68824212 | MS.gene21036:intron |
| AGGCATTGGGATAAGGATTG+AGG | 0.564277 | 1.4:-68818433 | MS.gene21036:CDS |
| CCGACAAATACACAACCATT+CGG | 0.567790 | 1.4:-68821997 | MS.gene21036:intron |
| AGTTGGGTTCAGCTTATGGT+AGG | 0.568189 | 1.4:+68821151 | None:intergenic |
| ATGGCTGAAGTTGTTTAGCA+AGG | 0.569030 | 1.4:+68817256 | None:intergenic |
| GGGAAAGCACTTACAACACC+TGG | 0.570813 | 1.4:+68820928 | None:intergenic |
| AACCCCTGCATCTCACCCGC+AGG | 0.573533 | 1.4:-68826177 | MS.gene21036:intron |
| ATTAGGCCCACCACCAACCA+CGG | 0.575070 | 1.4:+68823213 | None:intergenic |
| CAATCCTTATCCCAATGCCT+TGG | 0.576534 | 1.4:+68818436 | None:intergenic |
| TTGCTAAACAACTTCAGCCA+TGG | 0.577584 | 1.4:-68817254 | MS.gene21036:CDS |
| TCAATCCCGTGGTTGGTGGT+GGG | 0.577700 | 1.4:-68823219 | MS.gene21036:CDS |
| TGAGAGGTGCGCAGCGAATG+GGG | 0.579429 | 1.4:-68823450 | MS.gene21036:intron |
| GTACCTGCGGGTGAGATGCA+GGG | 0.580749 | 1.4:+68826174 | None:intergenic |
| TACCTGCGGGTGAGATGCAG+GGG | 0.581809 | 1.4:+68826175 | None:intergenic |
| GACATTGGATGTGAGTTACA+TGG | 0.582230 | 1.4:-68823056 | MS.gene21036:CDS |
| TGATAGCCAAACTCTTCTCT+GGG | 0.583126 | 1.4:+68825197 | None:intergenic |
| TCTCATTGGATACGCCTCGT+TGG | 0.585869 | 1.4:+68824149 | None:intergenic |
| AAAGATGGGGAGATCACACC+AGG | 0.587344 | 1.4:-68825271 | MS.gene21036:CDS |
| ATCAGATTCAATCCCGTGGT+TGG | 0.587531 | 1.4:-68823226 | MS.gene21036:intron |
| TTAGGCCCACCACCAACCAC+GGG | 0.589559 | 1.4:+68823214 | None:intergenic |
| AGCTTACTGTAACAACGTAA+AGG | 0.590674 | 1.4:-68823799 | MS.gene21036:CDS |
| ACAACTTCAGCCATGGAATG+GGG | 0.593125 | 1.4:-68817247 | MS.gene21036:CDS |
| CTGCCAACAACCTGGTGGTG+TGG | 0.593769 | 1.4:-68825092 | MS.gene21036:CDS |
| TCGTTCTTATTCCAGTCACA+AGG | 0.594752 | 1.4:-68826222 | MS.gene21036:CDS |
| ATTACAGGCTGCCAACAACC+TGG | 0.596169 | 1.4:-68825100 | MS.gene21036:CDS |
| CAGGTTGAAATGCTGCAGAA+AGG | 0.597743 | 1.4:-68821215 | MS.gene21036:CDS |
| ACGCGTACCTGGCCACCTTG+TGG | 0.599158 | 1.4:-68823017 | MS.gene21036:CDS |
| CAGGTAATAACTATTGAACC+AGG | 0.600635 | 1.4:-68819915 | MS.gene21036:CDS |
| TCCAGGTCACTATCTAGGAA+TGG | 0.601316 | 1.4:-68820995 | MS.gene21036:intron |
| AGAAACTAACAAGCATTGTG+TGG | 0.607256 | 1.4:-68822045 | MS.gene21036:CDS |
| GGTTGGTTGTCCAACATCAA+CGG | 0.613022 | 1.4:+68826196 | None:intergenic |
| ATCCTTCCAGATATGATAAG+GGG | 0.625213 | 1.4:-68823894 | MS.gene21036:CDS |
| AATGCTGCAGAAAGGACTCA+AGG | 0.627439 | 1.4:-68821207 | MS.gene21036:CDS |
| TTATTCTAGGAACGATCAGA+AGG | 0.629437 | 1.4:-68823176 | MS.gene21036:intron |
| CTGTATCAGATTCAATCCCG+TGG | 0.633552 | 1.4:-68823230 | MS.gene21036:intron |
| CAACTTCAGCCATGGAATGG+GGG | 0.635710 | 1.4:-68817246 | MS.gene21036:CDS |
| ATCAGCATATACTGAACTGG+AGG | 0.641525 | 1.4:-68823835 | MS.gene21036:CDS |
| ATTGTAGCTTCTAAAAGATG+GGG | 0.641791 | 1.4:-68825284 | MS.gene21036:intron |
| ATTTGTCGGATACTAGCACC+AGG | 0.642966 | 1.4:+68822011 | None:intergenic |
| AACCCCTTATCATATCTGGA+AGG | 0.645748 | 1.4:+68823892 | None:intergenic |
| GATCTCTAATGCAGGTACCA+AGG | 0.651356 | 1.4:-68818453 | MS.gene21036:intron |
| CACACCAGGCATATCTAGTG+AGG | 0.673963 | 1.4:-68825257 | MS.gene21036:CDS |
| ATCTGTCATCATCTTTACTG+GGG | 0.686632 | 1.4:+68825165 | None:intergenic |
| ACATTGGATGTGAGTTACAT+GGG | 0.693361 | 1.4:-68823055 | MS.gene21036:CDS |
| GAATACGAATGCAAAATGAG+AGG | 0.696219 | 1.4:-68823466 | MS.gene21036:CDS |
| GCAGAAGAGAAGCTACCACA+AGG | 0.709712 | 1.4:+68823002 | None:intergenic |
| GAAGAGAAGCTACCACAAGG+TGG | 0.733869 | 1.4:+68823005 | None:intergenic |
| TCCAATGAGAAAGTTGAACG+AGG | 0.743293 | 1.4:-68824136 | MS.gene21036:intron |
| ACAGGCTGCCAACAACCTGG+TGG | 0.744058 | 1.4:-68825097 | MS.gene21036:CDS |
| GCTGCAGAAAGGACTCAAGG+AGG | 0.745589 | 1.4:-68821204 | MS.gene21036:CDS |
| GAATACATTCAAGGCCAACG+AGG | 0.787952 | 1.4:-68824163 | MS.gene21036:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 68817242 | 68826421 | 68817242 | ID=MS.gene21036 |
| chr1.4 | mRNA | 68817242 | 68826421 | 68817242 | ID=MS.gene21036.t1;Parent=MS.gene21036 |
| chr1.4 | exon | 68826374 | 68826421 | 68826374 | ID=MS.gene21036.t1.exon1;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68826374 | 68826421 | 68826374 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68826178 | 68826255 | 68826178 | ID=MS.gene21036.t1.exon2;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68826178 | 68826255 | 68826178 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68825036 | 68825299 | 68825036 | ID=MS.gene21036.t1.exon3;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68825036 | 68825299 | 68825036 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68824137 | 68824229 | 68824137 | ID=MS.gene21036.t1.exon4;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68824137 | 68824229 | 68824137 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68823710 | 68823916 | 68823710 | ID=MS.gene21036.t1.exon5;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68823710 | 68823916 | 68823710 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68823451 | 68823554 | 68823451 | ID=MS.gene21036.t1.exon6;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68823451 | 68823554 | 68823451 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68823177 | 68823243 | 68823177 | ID=MS.gene21036.t1.exon7;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68823177 | 68823243 | 68823177 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68822998 | 68823105 | 68822998 | ID=MS.gene21036.t1.exon8;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68822998 | 68823105 | 68822998 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68821998 | 68822090 | 68821998 | ID=MS.gene21036.t1.exon9;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68821998 | 68822090 | 68821998 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68821144 | 68821234 | 68821144 | ID=MS.gene21036.t1.exon10;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68821144 | 68821234 | 68821144 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68820942 | 68821012 | 68820942 | ID=MS.gene21036.t1.exon11;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68820942 | 68821012 | 68820942 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68819879 | 68819934 | 68819879 | ID=MS.gene21036.t1.exon12;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68819879 | 68819934 | 68819879 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68818340 | 68818461 | 68818340 | ID=MS.gene21036.t1.exon13;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68818340 | 68818461 | 68818340 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| chr1.4 | exon | 68817242 | 68817321 | 68817242 | ID=MS.gene21036.t1.exon14;Parent=MS.gene21036.t1 |
| chr1.4 | CDS | 68817242 | 68817321 | 68817242 | ID=cds.MS.gene21036.t1;Parent=MS.gene21036.t1 |
| Gene Sequence |
| Protein sequence |
