AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21361


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21361.t1 MTR_1g090190 89.444 180 13 2 1 175 1 179 1.99e-107 305
MS.gene21361.t1 MTR_3g099580 42.609 115 65 1 4 118 8 121 5.91e-28 103
MS.gene21361.t1 MTR_2g088990 39.474 114 64 2 3 115 9 118 8.68e-28 103
MS.gene21361.t1 MTR_8g007035 40.517 116 61 2 4 117 11 120 3.40e-27 101
MS.gene21361.t1 MTR_3g099980 40.171 117 65 3 5 119 11 124 6.05e-26 99.0
MS.gene21361.t1 MTR_3g099680 40.171 117 65 3 5 119 11 124 8.44e-26 98.6
MS.gene21361.t1 MTR_8g007020 32.022 178 101 4 4 167 11 182 1.14e-25 97.4
MS.gene21361.t1 MTR_3g099570 34.752 141 83 2 5 145 11 142 2.97e-25 96.3
MS.gene21361.t1 MTR_6g080660 36.441 118 71 1 1 118 10 123 2.61e-24 94.7
MS.gene21361.t1 MTR_5g006040 30.435 161 104 3 1 153 4 164 2.18e-23 91.7
MS.gene21361.t1 MTR_3g099540 35.938 128 79 1 1 128 8 132 5.41e-22 86.7
MS.gene21361.t1 MTR_4g114870 42.574 101 53 2 22 117 190 290 7.70e-22 90.9
MS.gene21361.t1 MTR_4g114870 33.824 136 83 3 4 134 10 143 2.29e-16 75.9
MS.gene21361.t1 MTR_6g013420 38.971 136 77 4 4 136 19 151 3.03e-21 85.9
MS.gene21361.t1 MTR_1g104800 34.167 120 75 3 1 117 4 122 5.78e-21 85.1
MS.gene21361.t1 MTR_6g013200 33.962 159 95 5 4 157 16 169 6.88e-21 84.7
MS.gene21361.t1 MTR_6g023760 39.394 99 59 1 20 117 23 121 6.21e-20 82.4
MS.gene21361.t1 MTR_1g112700 34.058 138 79 4 4 130 14 150 1.91e-18 78.6
MS.gene21361.t1 MTR_2g025580 28.994 169 113 5 4 170 8 171 2.40e-18 78.2
MS.gene21361.t1 MTR_8g099220 35.938 128 69 4 2 118 3 128 5.72e-17 73.2
MS.gene21361.t1 MTR_8g463180 36.364 121 73 3 1 117 1 121 6.81e-17 76.6
MS.gene21361.t1 MTR_8g086360 33.571 140 85 4 4 136 6 144 6.89e-17 74.7
MS.gene21361.t1 MTR_0334s0010 34.127 126 75 2 1 118 1 126 1.15e-16 74.3
MS.gene21361.t1 MTR_6g022170 38.462 104 58 2 19 117 30 132 1.79e-16 73.2
MS.gene21361.t1 MTR_8g089110 36.842 114 68 3 4 116 9 119 4.47e-16 70.9
MS.gene21361.t1 MTR_1g014120 32.867 143 83 5 4 135 9 149 1.02e-15 71.6
MS.gene21361.t1 MTR_1g090420 30.263 152 96 4 5 152 10 155 4.74e-15 70.5
MS.gene21361.t1 MTR_4g130780 31.061 132 83 4 5 128 9 140 9.55e-15 68.9
MS.gene21361.t1 MTR_8g094990 32.593 135 82 4 4 130 7 140 1.44e-14 70.1
MS.gene21361.t1 MTR_4g130800 34.454 119 70 4 5 115 9 127 1.75e-14 69.7
MS.gene21361.t1 MTR_2g090575 33.577 137 80 4 4 130 7 142 1.94e-14 70.1
MS.gene21361.t1 MTR_6g083240 30.818 159 96 6 20 170 24 176 5.31e-14 66.6
MS.gene21361.t1 MTR_7g086100 29.268 123 84 2 2 121 5 127 5.60e-14 67.8
MS.gene21361.t1 MTR_3g089035 35.294 102 62 3 17 117 27 125 9.60e-14 64.7
MS.gene21361.t1 MTR_4g124280 33.333 138 73 4 20 143 22 154 1.14e-13 67.0
MS.gene21361.t1 MTR_7g086090 29.310 116 81 1 4 118 7 122 1.99e-13 66.2
MS.gene21361.t1 MTR_2g101300 29.787 141 89 4 9 142 11 148 2.79e-13 65.1
MS.gene21361.t1 MTR_1g077790 36.190 105 61 3 23 121 29 133 3.40e-13 65.1
MS.gene21361.t1 MTR_4g081100 32.710 107 67 2 19 120 28 134 4.01e-13 65.1
MS.gene21361.t1 MTR_1g105130 28.926 121 84 2 3 121 5 125 4.14e-13 63.9
MS.gene21361.t1 MTR_1g105120 28.926 121 84 2 3 121 5 125 4.14e-13 63.9
MS.gene21361.t1 MTR_7g086160 31.405 121 80 3 4 121 7 127 4.60e-13 63.9
MS.gene21361.t1 MTR_2g079030 33.333 132 83 4 2 130 5 134 4.94e-13 66.6
MS.gene21361.t1 MTR_3g105930 33.628 113 67 3 26 130 31 143 8.09e-13 63.5
MS.gene21361.t1 MTR_2g043730 36.207 116 68 4 4 116 11 123 8.60e-13 62.4
MS.gene21361.t1 MTR_4g077787 33.333 123 74 3 4 119 12 133 1.05e-12 63.5
MS.gene21361.t1 MTR_4g130800 36.364 99 57 3 23 115 28 126 1.18e-12 64.7
MS.gene21361.t1 MTR_4g078410 30.597 134 86 3 4 134 6 135 2.58e-12 62.4
MS.gene21361.t1 MTR_2g083250 34.259 108 64 3 20 120 23 130 3.07e-12 63.2
MS.gene21361.t1 MTR_4g111640 24.294 177 118 4 4 173 12 179 4.13e-12 61.6
MS.gene21361.t1 MTR_7g086280 28.448 116 82 1 4 118 7 122 9.94e-12 60.8
MS.gene21361.t1 MTR_7g086140 30.000 120 82 2 4 121 7 126 1.18e-11 60.1
MS.gene21361.t1 MTR_7g086190 30.000 120 82 2 4 121 7 126 1.18e-11 60.1
MS.gene21361.t1 MTR_3g089005 30.508 118 77 4 1 116 10 124 2.81e-11 58.5
MS.gene21361.t1 MTR_8g095013 31.356 118 76 3 4 117 7 123 4.20e-11 60.5
MS.gene21361.t1 MTR_8g088830 36.364 99 58 4 20 116 25 120 4.50e-11 57.8
MS.gene21361.t1 MTR_5g088000 27.007 137 93 4 4 137 2 134 5.76e-11 57.8
MS.gene21361.t1 MTR_7g086230 26.724 116 84 1 4 118 7 122 8.04e-11 58.2
MS.gene21361.t1 MTR_4g007250 30.693 101 68 2 19 118 25 124 8.06e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21361.t1 AT5G07475 50.314 159 75 3 1 158 7 162 2.31e-49 158
MS.gene21361.t1 AT2G32300 43.590 117 65 1 2 117 5 121 1.67e-30 112
MS.gene21361.t1 AT2G32300 43.590 117 65 1 2 117 35 151 5.46e-30 111
MS.gene21361.t1 AT2G31050 40.310 129 70 4 4 130 13 136 7.13e-24 93.2
MS.gene21361.t1 AT5G26330 39.604 101 58 2 20 117 21 121 3.68e-21 85.9
MS.gene21361.t1 AT3G60270 38.776 98 60 0 20 117 23 120 4.36e-21 85.5
MS.gene21361.t1 AT3G27200 35.833 120 73 3 1 117 3 121 4.52e-21 85.1
MS.gene21361.t1 AT2G25060 38.411 151 71 5 4 136 16 162 8.75e-21 84.7
MS.gene21361.t1 AT2G26720 36.207 116 69 3 4 117 13 125 9.19e-21 85.1
MS.gene21361.t1 AT5G25090 38.596 114 65 2 9 117 13 126 3.01e-20 83.6
MS.gene21361.t1 AT4G31840 34.752 141 82 5 5 135 6 146 1.12e-17 76.6
MS.gene21361.t1 AT3G60280 37.755 98 61 0 20 117 21 118 1.14e-17 77.4
MS.gene21361.t1 AT3G20570 40.000 105 59 2 20 120 27 131 2.31e-17 76.3
MS.gene21361.t1 AT1G72230 30.508 118 80 1 31 146 31 148 9.21e-17 74.3
MS.gene21361.t1 AT3G17675 30.769 117 78 2 4 117 11 127 1.02e-16 72.8
MS.gene21361.t1 AT5G53870 33.333 120 69 3 5 113 4 123 1.93e-16 75.9
MS.gene21361.t1 AT5G53870 33.333 120 69 3 5 113 4 123 1.95e-16 75.9
MS.gene21361.t1 AT2G44790 38.000 100 56 2 20 117 29 124 1.67e-15 71.2
MS.gene21361.t1 AT2G23990 36.029 136 74 5 9 131 13 148 2.69e-15 70.9
MS.gene21361.t1 AT4G28365 28.571 154 101 3 5 149 11 164 3.60e-15 70.5
MS.gene21361.t1 AT1G45063 33.333 126 76 4 1 120 6 129 3.94e-15 71.6
MS.gene21361.t1 AT1G45063 33.333 120 72 5 16 130 142 258 1.31e-11 61.6
MS.gene21361.t1 AT1G45063 33.333 126 76 4 1 120 6 129 6.06e-15 71.6
MS.gene21361.t1 AT1G45063 33.333 120 72 5 16 130 142 258 1.89e-11 61.6
MS.gene21361.t1 AT1G79800 34.454 119 66 4 23 130 34 151 6.28e-15 69.7
MS.gene21361.t1 AT4G27520 35.897 117 68 3 4 117 16 128 2.20e-14 70.1
MS.gene21361.t1 AT4G12880 32.479 117 75 3 5 118 11 126 2.89e-14 66.6
MS.gene21361.t1 AT1G64640 36.364 121 70 3 19 132 29 149 3.33e-14 67.8
MS.gene21361.t1 AT3G53330 33.607 122 76 4 23 139 188 309 5.76e-14 68.6
MS.gene21361.t1 AT3G18590 30.894 123 77 3 2 117 5 126 2.63e-13 65.1
MS.gene21361.t1 AT4G30590 34.188 117 70 4 4 113 8 124 6.16e-13 64.3
MS.gene21361.t1 AT1G22480 32.231 121 76 2 1 120 1 116 8.92e-13 63.5
MS.gene21361.t1 AT5G57920 34.783 92 53 2 37 127 42 127 1.58e-12 63.2
MS.gene21361.t1 AT4G32490 33.058 121 76 3 4 121 14 132 1.90e-12 63.5
MS.gene21361.t1 AT5G57920 34.783 92 53 2 37 127 41 126 3.24e-12 62.0
MS.gene21361.t1 AT5G57920 32.710 107 61 3 26 127 59 159 3.76e-12 62.4
MS.gene21361.t1 AT3G01070 38.938 113 66 2 9 118 13 125 4.95e-12 61.2
MS.gene21361.t1 AT1G48940 31.707 123 76 4 2 117 4 125 5.85e-11 58.5

Find 28 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTGCTCTTCTTTGTTGA+TGG 0.310086 1.4:+71510248 None:intergenic
AATCTGTAGCATCCCATAAA+AGG 0.320012 1.4:+71510169 None:intergenic
TATAGTTTATATTGCTGTTA+TGG 0.320304 1.4:-71510195 MS.gene21361:CDS
TCTGTTCCAGCTACTGCTTT+TGG 0.352278 1.4:+71510284 None:intergenic
ACAAAAGTGTTGGCAAATTA+CGG 0.407043 1.4:-71510446 MS.gene21361:CDS
GTGTTGGCAAATTACGGAAA+TGG 0.435345 1.4:-71510440 MS.gene21361:CDS
CATTGACAAGAGCCGGAGAC+AGG 0.497365 1.4:-71510403 MS.gene21361:CDS
CTGTCTCCGGCTCTTGTCAA+TGG 0.513474 1.4:+71510404 None:intergenic
CTGTTGGAGATAGCTCTGGC+TGG 0.524284 1.4:-71510912 MS.gene21361:CDS
CTAGCACCAAAAGCAGTAGC+TGG 0.525300 1.4:-71510290 MS.gene21361:CDS
TACACTGTTGGAGATAGCTC+TGG 0.540204 1.4:-71510916 MS.gene21361:CDS
AACAAACTGTATTGCCTTGG+TGG 0.541279 1.4:-71510365 MS.gene21361:CDS
ACATACAAAATACCTGTCTC+CGG 0.555623 1.4:+71510391 None:intergenic
ACGGTGCCATTGACAAGAGC+CGG 0.574751 1.4:-71510410 MS.gene21361:CDS
ATGCAACACAACAAAAGTGT+TGG 0.577530 1.4:-71510456 MS.gene21361:CDS
TCAGCAACTACCTACACTGT+TGG 0.586019 1.4:-71510928 MS.gene21361:CDS
TGTTGGAGATAGCTCTGGCT+GGG 0.608785 1.4:-71510911 MS.gene21361:CDS
AGAGCTATCTCCAACAGTGT+AGG 0.611707 1.4:+71510918 None:intergenic
CATGTTCATGTAGAAGATGA+CGG 0.616290 1.4:-71510332 MS.gene21361:CDS
CAGTAGCTGGAACAGATCAA+AGG 0.619028 1.4:-71510277 MS.gene21361:CDS
ATCGACATACAGTGTGGACG+AGG 0.624451 1.4:-71510501 MS.gene21361:CDS
TTAGCTCTAATCTAGAAACA+TGG 0.627015 1.4:-71510885 MS.gene21361:CDS
TAGCTCTAATCTAGAAACAT+GGG 0.636260 1.4:-71510884 MS.gene21361:CDS
GGAAACAAACTGTATTGCCT+TGG 0.642701 1.4:-71510368 MS.gene21361:CDS
ACAAACTGTATTGCCTTGGT+GGG 0.646382 1.4:-71510364 MS.gene21361:CDS
CATGAAGTTTCATCCCACCA+AGG 0.728162 1.4:+71510351 None:intergenic
ATATTCATCGACATACAGTG+TGG 0.739211 1.4:-71510507 MS.gene21361:CDS
TTACGGAAATGGAAACACGA+CGG 0.772125 1.4:-71510429 MS.gene21361:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATATTTGTATTTTTGA+GGG - chr1.4:71510460-71510479 MS.gene21361:CDS 10.0%
!!! TATTTATATTTGTATTTTTG+AGG - chr1.4:71510459-71510478 MS.gene21361:CDS 10.0%
!! AATTATTGAATGAAAGATGA+AGG + chr1.4:71510333-71510352 None:intergenic 20.0%
!! ATGAATATTGAAAAACTGAA+AGG + chr1.4:71510629-71510648 None:intergenic 20.0%
!!! AACAGATTTTATGCATTTAT+AGG - chr1.4:71510562-71510581 MS.gene21361:intron 20.0%
!!! TATAGTTTATATTGCTGTTA+TGG - chr1.4:71510953-71510972 MS.gene21361:CDS 20.0%
!!! TGTTTTTATTAATCCATAGA+TGG + chr1.4:71510430-71510449 None:intergenic 20.0%
! GCTGATAAAAATTTCAAGAT+AGG - chr1.4:71510289-71510308 MS.gene21361:CDS 25.0%
! TAACAAAAAAAGTTCCCAAA+TGG + chr1.4:71510601-71510620 None:intergenic 25.0%
GACAACATTTACAACCATTT+GGG - chr1.4:71510584-71510603 MS.gene21361:intron 30.0%
! TCTTTCTCTTCTGTTTTCTT+TGG - chr1.4:71510183-71510202 MS.gene21361:CDS 30.0%
!! AAGAGCACAGAATTTTCAAA+GGG - chr1.4:71510912-71510931 MS.gene21361:CDS 30.0%
!! ACAAAAGTGTTGGCAAATTA+CGG - chr1.4:71510702-71510721 MS.gene21361:intron 30.0%
!! TAGCTCTAATCTAGAAACAT+GGG - chr1.4:71510264-71510283 MS.gene21361:CDS 30.0%
!! TTAGCTCTAATCTAGAAACA+TGG - chr1.4:71510263-71510282 MS.gene21361:CDS 30.0%
AATCTGTAGCATCCCATAAA+AGG + chr1.4:71510982-71511001 None:intergenic 35.0%
ACATACAAAATACCTGTCTC+CGG + chr1.4:71510760-71510779 None:intergenic 35.0%
ATATTCATCGACATACAGTG+TGG - chr1.4:71510641-71510660 MS.gene21361:intron 35.0%
CATGTTCATGTAGAAGATGA+CGG - chr1.4:71510816-71510835 MS.gene21361:intron 35.0%
GGACAACATTTACAACCATT+TGG - chr1.4:71510583-71510602 MS.gene21361:intron 35.0%
! ATGCAACACAACAAAAGTGT+TGG - chr1.4:71510692-71510711 MS.gene21361:intron 35.0%
! CTGTTATGGTAGCCTTTTAT+GGG - chr1.4:71510967-71510986 MS.gene21361:CDS 35.0%
!! GAAGAGCACAGAATTTTCAA+AGG - chr1.4:71510911-71510930 MS.gene21361:CDS 35.0%
AACAAACTGTATTGCCTTGG+TGG - chr1.4:71510783-71510802 MS.gene21361:intron 40.0%
ACAAACTGTATTGCCTTGGT+GGG - chr1.4:71510784-71510803 MS.gene21361:intron 40.0%
ATTCTCTCTGTGACCATCTA+TGG - chr1.4:71510414-71510433 MS.gene21361:CDS 40.0%
GGAAACAAACTGTATTGCCT+TGG - chr1.4:71510780-71510799 MS.gene21361:intron 40.0%
TCTGTGCTCTTCTTTGTTGA+TGG + chr1.4:71510903-71510922 None:intergenic 40.0%
TTACGGAAATGGAAACACGA+CGG - chr1.4:71510719-71510738 MS.gene21361:intron 40.0%
! GCTGTTATGGTAGCCTTTTA+TGG - chr1.4:71510966-71510985 MS.gene21361:CDS 40.0%
! GGAGACAGGTATTTTGTATG+TGG - chr1.4:71510759-71510778 MS.gene21361:intron 40.0%
! GTGTTGGCAAATTACGGAAA+TGG - chr1.4:71510708-71510727 MS.gene21361:intron 40.0%
!!! ACTGCTTTTGGTGCTAGATT+TGG + chr1.4:71510855-71510874 None:intergenic 40.0%
AGAGCTATCTCCAACAGTGT+AGG + chr1.4:71510233-71510252 None:intergenic 45.0%
CAGTAGCTGGAACAGATCAA+AGG - chr1.4:71510871-71510890 MS.gene21361:CDS 45.0%
CATGAAGTTTCATCCCACCA+AGG + chr1.4:71510800-71510819 None:intergenic 45.0%
TACACTGTTGGAGATAGCTC+TGG - chr1.4:71510232-71510251 MS.gene21361:CDS 45.0%
TCAGCAACTACCTACACTGT+TGG - chr1.4:71510220-71510239 MS.gene21361:CDS 45.0%
! TCTGTTCCAGCTACTGCTTT+TGG + chr1.4:71510867-71510886 None:intergenic 45.0%
ATCGACATACAGTGTGGACG+AGG - chr1.4:71510647-71510666 MS.gene21361:intron 50.0%
CTAGCACCAAAAGCAGTAGC+TGG - chr1.4:71510858-71510877 MS.gene21361:CDS 50.0%
!! TGTTGGAGATAGCTCTGGCT+GGG - chr1.4:71510237-71510256 MS.gene21361:CDS 50.0%
CATTGACAAGAGCCGGAGAC+AGG - chr1.4:71510745-71510764 MS.gene21361:intron 55.0%
CTGTCTCCGGCTCTTGTCAA+TGG + chr1.4:71510747-71510766 None:intergenic 55.0%
!! ACGGTGCCATTGACAAGAGC+CGG - chr1.4:71510738-71510757 MS.gene21361:intron 55.0%
!! CTGTTGGAGATAGCTCTGGC+TGG - chr1.4:71510236-71510255 MS.gene21361:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 71510166 71511004 71510166 ID=MS.gene21361
chr1.4 mRNA 71510166 71511004 71510166 ID=MS.gene21361.t1;Parent=MS.gene21361
chr1.4 exon 71510848 71511004 71510848 ID=MS.gene21361.t1.exon1;Parent=MS.gene21361.t1
chr1.4 CDS 71510848 71511004 71510848 ID=cds.MS.gene21361.t1;Parent=MS.gene21361.t1
chr1.4 exon 71510166 71510536 71510166 ID=MS.gene21361.t1.exon2;Parent=MS.gene21361.t1
chr1.4 CDS 71510166 71510536 71510166 ID=cds.MS.gene21361.t1;Parent=MS.gene21361.t1
Gene Sequence

>MS.gene21361

ATGGAAAAGTTGCTTCTTCTTTCTCTTCTGTTTTCTTTGGCAACTATCACATGTTCAGCAACTACCTACACTGTTGGAGATAGCTCTGGCTGGGACATTAGCTCTAATCTAGAAACATGGGTTGCTGATAAAAATTTCAAGATAGGTGATGCTCTTCGTAAGTACCTTCATCTTTCATTCAATAATTCATTTTCTATAAGCTATTCAAATCATACATATATACTTACAAGACATATCAAAAATCAATCATTCTCTCTGTGACCATCTATGGATTAATAAAAACATATTTTATGTATTTATATTTGTATTTTTGAGGGTTAAAATTCAATAGATAATGAAACTTGAAATGTCAAGTTGATTATCGTTTATAGCTTTTGATAACAAGATTTATGATAGAACAGATTTTATGCATTTATAGGACAACATTTACAACCATTTGGGAACTTTTTTTGTTATTTTTCCTTTCAGTTTTTCAATATTCATCGACATACAGTGTGGACGAGGTTACAAAAGAAAACTTCGACACATGCAACACAACAAAAGTGTTGGCAAATTACGGAAATGGAAACACGACGGTGCCATTGACAAGAGCCGGAGACAGGTATTTTGTATGTGGAAACAAACTGTATTGCCTTGGTGGGATGAAACTTCATGTTCATGTAGAAGATGACGGCAAATCAATTTCGCCAAATCTAGCACCAAAAGCAGTAGCTGGAACAGATCAAAGGACTTCTCCATCAACAAAGAAGAGCACAGAATTTTCAAAGGGAGTTGCAAATTGTGCACATATAGTTTATATTGCTGTTATGGTAGCCTTTTATGGGATGCTACAGATTTGA

Protein sequence

>MS.gene21361.t1

MEKLLLLSLLFSLATITCSATTYTVGDSSGWDISSNLETWVADKNFKIGDALLFQYSSTYSVDEVTKENFDTCNTTKVLANYGNGNTTVPLTRAGDRYFVCGNKLYCLGGMKLHVHVEDDGKSISPNLAPKAVAGTDQRTSPSTKKSTEFSKGVANCAHIVYIAVMVAFYGMLQI