Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21467.t1 | XP_003611481.1 | 96.9 | 193 | 5 | 1 | 19 | 211 | 60 | 251 | 4.10E-101 | 377.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21467.t1 | O80942 | 64.8 | 71 | 19 | 2 | 19 | 85 | 19 | 87 | 1.1e-17 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21467.t1 | D7R6G3 | 96.9 | 193 | 5 | 1 | 19 | 211 | 60 | 251 | 3.0e-101 | 377.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene21467.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059669 | MS.gene21467 | 0.863028 | 3.33E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21467.t1 | MTR_5g014400 | 95.735 | 211 | 5 | 2 | 1 | 211 | 45 | 251 | 5.35e-149 | 414 |
| MS.gene21467.t1 | MTR_5g080660 | 36.726 | 226 | 107 | 10 | 8 | 207 | 14 | 229 | 6.74e-26 | 100 |
| MS.gene21467.t1 | MTR_4g021270 | 38.378 | 185 | 79 | 7 | 17 | 171 | 35 | 214 | 1.82e-24 | 99.0 |
| MS.gene21467.t1 | MTR_3g068095 | 73.684 | 57 | 14 | 1 | 18 | 73 | 33 | 89 | 3.71e-24 | 96.7 |
| MS.gene21467.t1 | MTR_2g076060 | 87.805 | 41 | 5 | 0 | 36 | 76 | 47 | 87 | 8.37e-22 | 89.4 |
| MS.gene21467.t1 | MTR_1g070265 | 80.000 | 40 | 8 | 0 | 34 | 73 | 30 | 69 | 4.74e-18 | 78.6 |
| MS.gene21467.t1 | MTR_1g070250 | 31.977 | 172 | 97 | 5 | 39 | 208 | 38 | 191 | 1.47e-14 | 69.3 |
| MS.gene21467.t1 | MTR_7g063220 | 76.471 | 34 | 8 | 0 | 39 | 72 | 24 | 57 | 8.94e-14 | 66.2 |
| MS.gene21467.t1 | MTR_4g064873 | 32.749 | 171 | 85 | 4 | 38 | 208 | 20 | 160 | 1.02e-13 | 67.0 |
| MS.gene21467.t1 | MTR_1g075430 | 31.551 | 187 | 88 | 9 | 39 | 205 | 18 | 184 | 3.36e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21467.t1 | AT4G17810 | 42.411 | 224 | 86 | 9 | 1 | 208 | 7 | 203 | 2.27e-42 | 142 |
| MS.gene21467.t1 | AT4G17810 | 40.553 | 217 | 70 | 11 | 1 | 208 | 7 | 173 | 2.80e-34 | 120 |
| MS.gene21467.t1 | AT2G37740 | 57.303 | 89 | 26 | 3 | 1 | 85 | 7 | 87 | 2.86e-25 | 100 |
| MS.gene21467.t1 | AT5G06070 | 36.493 | 211 | 92 | 8 | 20 | 208 | 32 | 222 | 1.01e-23 | 94.7 |
| MS.gene21467.t1 | AT2G42410 | 34.667 | 225 | 93 | 8 | 9 | 205 | 14 | 212 | 1.76e-20 | 85.9 |
| MS.gene21467.t1 | AT2G42410 | 34.667 | 225 | 93 | 8 | 9 | 205 | 14 | 212 | 1.76e-20 | 85.9 |
| MS.gene21467.t1 | AT2G42410 | 34.667 | 225 | 93 | 8 | 9 | 205 | 40 | 238 | 3.29e-20 | 85.5 |
| MS.gene21467.t1 | AT3G23130 | 71.429 | 49 | 14 | 0 | 26 | 74 | 32 | 80 | 7.74e-20 | 84.0 |
| MS.gene21467.t1 | AT3G09290 | 73.529 | 34 | 9 | 0 | 39 | 72 | 33 | 66 | 7.39e-13 | 64.7 |
| MS.gene21467.t1 | AT3G53820 | 63.889 | 36 | 13 | 0 | 38 | 73 | 18 | 53 | 5.79e-11 | 58.9 |
Find 47 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAAGCATTATTATTATT+AGG | 0.154031 | 5.3:-7538679 | None:intergenic |
| TATTATTAGGGTTTGGTAAA+TGG | 0.178618 | 5.3:-7538666 | None:intergenic |
| ATTTGCATCATCAAATTATT+TGG | 0.195764 | 5.3:-7538778 | None:intergenic |
| GTTGCTGTTGGTGTTGTTTA+GGG | 0.261033 | 5.3:-7538363 | None:intergenic |
| TGTTGCTGTTGGTGTTGTTT+AGG | 0.267605 | 5.3:-7538364 | None:intergenic |
| CAAAAGCATTATTATTATTA+GGG | 0.268434 | 5.3:-7538678 | None:intergenic |
| TTAACGGTGGTTGGCTTTGA+TGG | 0.294750 | 5.3:-7538564 | None:intergenic |
| CTTGATCTTGAGCTCCGTTT+AGG | 0.296503 | 5.3:+7538946 | MS.gene21467:CDS |
| CTTCTTGTTCTTGTTGCTGT+TGG | 0.353443 | 5.3:-7538375 | None:intergenic |
| TACAATAGTTCTAGTCCTAA+TGG | 0.356996 | 5.3:+7538706 | MS.gene21467:CDS |
| TTGATCTTGAGCTCCGTTTA+GGG | 0.364351 | 5.3:+7538947 | MS.gene21467:CDS |
| GAGATTGAGAGAAAAGTAGA+AGG | 0.386084 | 5.3:-7538736 | None:intergenic |
| GTAGAAGGAGATTCTCCATT+AGG | 0.392764 | 5.3:-7538721 | None:intergenic |
| TTCATGACCGTTATCATCAA+TGG | 0.401936 | 5.3:-7538914 | None:intergenic |
| GCATTATTATTATTAGGGTT+TGG | 0.403821 | 5.3:-7538673 | None:intergenic |
| AACAAGAAGATGAAGATTCA+TGG | 0.412727 | 5.3:+7538389 | MS.gene21467:CDS |
| CAAGAGCTTGTTGCTAATGC+TGG | 0.417258 | 5.3:+7538628 | MS.gene21467:CDS |
| GTGTTGGCTTGTTCCCTAAA+CGG | 0.435644 | 5.3:-7538960 | None:intergenic |
| TCCACTTGAAAATTAAAAGA+TGG | 0.441538 | 5.3:-7538814 | None:intergenic |
| ATTAGGACTAGAACTATTGT+AGG | 0.449818 | 5.3:-7538704 | None:intergenic |
| ATGAAGATTCATGGGAGGTA+AGG | 0.450992 | 5.3:+7538398 | MS.gene21467:CDS |
| TTCCGGTCAGCTCAAGCTCT+TGG | 0.471669 | 5.3:+7538502 | MS.gene21467:CDS |
| GAGATGAATTTAACGGTGGT+TGG | 0.484111 | 5.3:-7538573 | None:intergenic |
| TGCATCATCAAATTATTTGG+AGG | 0.491401 | 5.3:-7538775 | None:intergenic |
| GGTTTGGTAAATGGTAAAGA+AGG | 0.506848 | 5.3:-7538657 | None:intergenic |
| CGGTCAGCTCAAGCTCTTGG+TGG | 0.515963 | 5.3:+7538505 | MS.gene21467:CDS |
| GATGGAGACGAGCACGGTCG+CGG | 0.516231 | 5.3:-7538546 | None:intergenic |
| ACAAGAAGATGAAGATTCAT+GGG | 0.528846 | 5.3:+7538390 | MS.gene21467:CDS |
| CACCAAGAGCTTGAGCTGAC+CGG | 0.533757 | 5.3:-7538504 | None:intergenic |
| TGAAGATTCATGGGAGGTAA+GGG | 0.539677 | 5.3:+7538399 | MS.gene21467:CDS |
| TGTGGAGGAATATTTATGAA+TGG | 0.545539 | 5.3:-7538607 | None:intergenic |
| GAAGGATGAGATGAATTTAA+CGG | 0.547030 | 5.3:-7538580 | None:intergenic |
| AGGTGCAAGTAGCATGTTGA+TGG | 0.551205 | 5.3:-7538891 | None:intergenic |
| TGCACCTCCATTGATGATAA+CGG | 0.570866 | 5.3:+7538907 | MS.gene21467:CDS |
| GCATTAGCAACAAGCTCTTG+TGG | 0.586992 | 5.3:-7538625 | None:intergenic |
| GGCTTTGATGGAGACGAGCA+CGG | 0.589738 | 5.3:-7538552 | None:intergenic |
| ATATTTATGAATGGTGATGA+AGG | 0.596989 | 5.3:-7538598 | None:intergenic |
| GTGCAGGTATAGGACCTTGG+TGG | 0.606792 | 5.3:-7538463 | None:intergenic |
| AAAGTGCAGGTATAGGACCT+TGG | 0.620732 | 5.3:-7538466 | None:intergenic |
| TTCAAGTGGATAATTCAGCT+AGG | 0.628424 | 5.3:+7538827 | MS.gene21467:CDS |
| ATGACCGTTATCATCAATGG+AGG | 0.643461 | 5.3:-7538911 | None:intergenic |
| TGAACACAACATGGCCACCA+AGG | 0.646071 | 5.3:+7538449 | MS.gene21467:CDS |
| GGAGACGAGCACGGTCGCGG+CGG | 0.650175 | 5.3:-7538543 | None:intergenic |
| AGAAGATGAAGATTCATGGG+AGG | 0.682315 | 5.3:+7538393 | MS.gene21467:CDS |
| TTAGCAACAAGCTCTTGTGG+AGG | 0.690594 | 5.3:-7538622 | None:intergenic |
| GGAATATTATGAACACAACA+TGG | 0.697427 | 5.3:+7538440 | MS.gene21467:CDS |
| GGATGAGATGAATTTAACGG+TGG | 0.714956 | 5.3:-7538577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAAAAGCATTATTATTATTA+GGG | - | chr5.3:7538681-7538700 | None:intergenic | 15.0% |
| !! | ATTTGCATCATCAAATTATT+TGG | - | chr5.3:7538781-7538800 | None:intergenic | 20.0% |
| !!! | GCAAAAGCATTATTATTATT+AGG | - | chr5.3:7538682-7538701 | None:intergenic | 20.0% |
| ! | GCATTATTATTATTAGGGTT+TGG | - | chr5.3:7538676-7538695 | None:intergenic | 25.0% |
| ! | TCCACTTGAAAATTAAAAGA+TGG | - | chr5.3:7538817-7538836 | None:intergenic | 25.0% |
| !! | ATATTTATGAATGGTGATGA+AGG | - | chr5.3:7538601-7538620 | None:intergenic | 25.0% |
| !! | TATTATTAGGGTTTGGTAAA+TGG | - | chr5.3:7538669-7538688 | None:intergenic | 25.0% |
| !!! | TCCATCTTTTAATTTTCAAG+TGG | + | chr5.3:7538813-7538832 | MS.gene21467:CDS | 25.0% |
| AACAAGAAGATGAAGATTCA+TGG | + | chr5.3:7538389-7538408 | MS.gene21467:CDS | 30.0% | |
| ACAAGAAGATGAAGATTCAT+GGG | + | chr5.3:7538390-7538409 | MS.gene21467:CDS | 30.0% | |
| ATTAGGACTAGAACTATTGT+AGG | - | chr5.3:7538707-7538726 | None:intergenic | 30.0% | |
| GAAGGATGAGATGAATTTAA+CGG | - | chr5.3:7538583-7538602 | None:intergenic | 30.0% | |
| GGAATATTATGAACACAACA+TGG | + | chr5.3:7538440-7538459 | MS.gene21467:CDS | 30.0% | |
| TGCATCATCAAATTATTTGG+AGG | - | chr5.3:7538778-7538797 | None:intergenic | 30.0% | |
| TGTGGAGGAATATTTATGAA+TGG | - | chr5.3:7538610-7538629 | None:intergenic | 30.0% | |
| !! | TACAATAGTTCTAGTCCTAA+TGG | + | chr5.3:7538706-7538725 | MS.gene21467:CDS | 30.0% |
| !! | TTCTTCTTTTTCTAGCAAAG+TGG | + | chr5.3:7538867-7538886 | MS.gene21467:CDS | 30.0% |
| GAGATTGAGAGAAAAGTAGA+AGG | - | chr5.3:7538739-7538758 | None:intergenic | 35.0% | |
| TTCAAGTGGATAATTCAGCT+AGG | + | chr5.3:7538827-7538846 | MS.gene21467:CDS | 35.0% | |
| TTCATGACCGTTATCATCAA+TGG | - | chr5.3:7538917-7538936 | None:intergenic | 35.0% | |
| ! | GGTTTGGTAAATGGTAAAGA+AGG | - | chr5.3:7538660-7538679 | None:intergenic | 35.0% |
| AGAAGATGAAGATTCATGGG+AGG | + | chr5.3:7538393-7538412 | MS.gene21467:CDS | 40.0% | |
| ATGAAGATTCATGGGAGGTA+AGG | + | chr5.3:7538398-7538417 | MS.gene21467:CDS | 40.0% | |
| ATGACCGTTATCATCAATGG+AGG | - | chr5.3:7538914-7538933 | None:intergenic | 40.0% | |
| CTTCTTGTTCTTGTTGCTGT+TGG | - | chr5.3:7538378-7538397 | None:intergenic | 40.0% | |
| GAGATGAATTTAACGGTGGT+TGG | - | chr5.3:7538576-7538595 | None:intergenic | 40.0% | |
| GGATGAGATGAATTTAACGG+TGG | - | chr5.3:7538580-7538599 | None:intergenic | 40.0% | |
| GTAGAAGGAGATTCTCCATT+AGG | - | chr5.3:7538724-7538743 | None:intergenic | 40.0% | |
| TGAAGATTCATGGGAGGTAA+GGG | + | chr5.3:7538399-7538418 | MS.gene21467:CDS | 40.0% | |
| TTGATCTTGAGCTCCGTTTA+GGG | + | chr5.3:7538947-7538966 | MS.gene21467:CDS | 40.0% | |
| ! | CTTTTTGCAGAAGAGAGTTC+CGG | + | chr5.3:7538485-7538504 | MS.gene21467:CDS | 40.0% |
| ! | TCTGCAAAAAGTGCAGGTAT+AGG | - | chr5.3:7538476-7538495 | None:intergenic | 40.0% |
| ! | TGCACCTCCATTGATGATAA+CGG | + | chr5.3:7538907-7538926 | MS.gene21467:CDS | 40.0% |
| !! | GTTGCTGTTGGTGTTGTTTA+GGG | - | chr5.3:7538366-7538385 | None:intergenic | 40.0% |
| !! | TGTTGCTGTTGGTGTTGTTT+AGG | - | chr5.3:7538367-7538386 | None:intergenic | 40.0% |
| AAAGTGCAGGTATAGGACCT+TGG | - | chr5.3:7538469-7538488 | None:intergenic | 45.0% | |
| AGGTGCAAGTAGCATGTTGA+TGG | - | chr5.3:7538894-7538913 | None:intergenic | 45.0% | |
| CTTGATCTTGAGCTCCGTTT+AGG | + | chr5.3:7538946-7538965 | MS.gene21467:CDS | 45.0% | |
| GCATTAGCAACAAGCTCTTG+TGG | - | chr5.3:7538628-7538647 | None:intergenic | 45.0% | |
| TTAGCAACAAGCTCTTGTGG+AGG | - | chr5.3:7538625-7538644 | None:intergenic | 45.0% | |
| ! | CTCTCTTCTGCAAAAAGTGC+AGG | - | chr5.3:7538482-7538501 | None:intergenic | 45.0% |
| !! | CAAGAGCTTGTTGCTAATGC+TGG | + | chr5.3:7538628-7538647 | MS.gene21467:CDS | 45.0% |
| !! | GTGTTGGCTTGTTCCCTAAA+CGG | - | chr5.3:7538963-7538982 | None:intergenic | 45.0% |
| !! | TTAACGGTGGTTGGCTTTGA+TGG | - | chr5.3:7538567-7538586 | None:intergenic | 45.0% |
| TGAACACAACATGGCCACCA+AGG | + | chr5.3:7538449-7538468 | MS.gene21467:CDS | 50.0% | |
| ! | GGGCTTTTGCTGAAGACACA+AGG | + | chr5.3:7538419-7538438 | MS.gene21467:CDS | 50.0% |
| CACCAAGAGCTTGAGCTGAC+CGG | - | chr5.3:7538507-7538526 | None:intergenic | 55.0% | |
| GTGCAGGTATAGGACCTTGG+TGG | - | chr5.3:7538466-7538485 | None:intergenic | 55.0% | |
| TTCCGGTCAGCTCAAGCTCT+TGG | + | chr5.3:7538502-7538521 | MS.gene21467:CDS | 55.0% | |
| ! | GGCTTTGATGGAGACGAGCA+CGG | - | chr5.3:7538555-7538574 | None:intergenic | 55.0% |
| CGGTCAGCTCAAGCTCTTGG+TGG | + | chr5.3:7538505-7538524 | MS.gene21467:CDS | 60.0% | |
| GATGGAGACGAGCACGGTCG+CGG | - | chr5.3:7538549-7538568 | None:intergenic | 65.0% | |
| GGAGACGAGCACGGTCGCGG+CGG | - | chr5.3:7538546-7538565 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 7538355 | 7538990 | 7538355 | ID=MS.gene21467 |
| chr5.3 | mRNA | 7538355 | 7538990 | 7538355 | ID=MS.gene21467.t1;Parent=MS.gene21467 |
| chr5.3 | exon | 7538355 | 7538990 | 7538355 | ID=MS.gene21467.t1.exon1;Parent=MS.gene21467.t1 |
| chr5.3 | CDS | 7538355 | 7538990 | 7538355 | ID=cds.MS.gene21467.t1;Parent=MS.gene21467.t1 |
| Gene Sequence |
| Protein sequence |
