Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21491.t1 | XP_013453459.1 | 83.8 | 105 | 17 | 0 | 1 | 105 | 1 | 105 | 3.30E-35 | 157.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21491.t1 | A0A072UNS2 | 83.8 | 105 | 17 | 0 | 1 | 105 | 1 | 105 | 2.4e-35 | 157.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene21491 | 0.811011 | 8.67E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene21491 | -0.804239 | 2.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21491.t1 | MTR_5g013975 | 98.095 | 105 | 2 | 0 | 1 | 105 | 1 | 105 | 9.89e-72 | 208 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21491.t1 | AT3G59840 | 58.095 | 105 | 36 | 2 | 1 | 105 | 1 | 97 | 1.58e-34 | 114 |
Find 35 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGATAAGGTTACTCTTGC+TGG | 0.280094 | 5.3:+7131765 | MS.gene21491:CDS |
| GTCGTCGATGACTTCGCTTC+AGG | 0.338584 | 5.3:-7131603 | None:intergenic |
| GGTTCAAGATCTTCAGAAAC+TGG | 0.373697 | 5.3:-7131795 | None:intergenic |
| ATCCCTGGCGCCTCCGTGGT+TGG | 0.398567 | 5.3:-7131666 | None:intergenic |
| AAACCAACCACGGAGGCGCC+AGG | 0.442569 | 5.3:+7131663 | MS.gene21491:CDS |
| GGTTGCTGCGAGCAACGATA+AGG | 0.463185 | 5.3:+7131752 | MS.gene21491:CDS |
| GTTTACTGTGGTGGCGGAAG+GGG | 0.469288 | 5.3:+7131568 | MS.gene21491:CDS |
| AGATCTTGAACCTTGTCGTT+GGG | 0.475907 | 5.3:+7131806 | MS.gene21491:CDS |
| AGCAAAGAATGGCGGTGCCT+CGG | 0.478420 | 5.3:+7131635 | MS.gene21491:CDS |
| TCCACTGCACCGTTCATCCC+TGG | 0.487594 | 5.3:-7131681 | None:intergenic |
| GGGTTTACTGTGGTGGCGGA+AGG | 0.492850 | 5.3:+7131566 | MS.gene21491:CDS |
| ACTGAAACACTCGCGTTCAA+AGG | 0.493969 | 5.3:-7131714 | None:intergenic |
| AAGATCTTGAACCTTGTCGT+TGG | 0.501854 | 5.3:+7131805 | MS.gene21491:CDS |
| ACGGTGCAGTGGAGGTTAAA+CGG | 0.502142 | 5.3:+7131691 | MS.gene21491:CDS |
| GGTTTACTGTGGTGGCGGAA+GGG | 0.516623 | 5.3:+7131567 | MS.gene21491:CDS |
| ACGGAGAACGCAGCAAAGAA+TGG | 0.516724 | 5.3:+7131624 | MS.gene21491:CDS |
| TTGTCGTTGGGAAATTCTGC+CGG | 0.548386 | 5.3:+7131818 | MS.gene21491:CDS |
| GGCGCGTTTGACTCAACCGC+CGG | 0.563952 | 5.3:-7131837 | None:intergenic |
| GAATGGGTTTACTGTGGTGG+CGG | 0.565035 | 5.3:+7131562 | None:intergenic |
| ACGGAGGCGCCAGGGATGAA+CGG | 0.590223 | 5.3:+7131672 | MS.gene21491:CDS |
| AGGGATGAACGGTGCAGTGG+AGG | 0.592078 | 5.3:+7131683 | MS.gene21491:CDS |
| TAAGAATGGGTTTACTGTGG+TGG | 0.597545 | 5.3:+7131559 | None:intergenic |
| GGCAGAATTTCCCAACGACA+AGG | 0.605086 | 5.3:-7131816 | None:intergenic |
| GTTCATCCCTGGCGCCTCCG+TGG | 0.608954 | 5.3:-7131670 | None:intergenic |
| AACCAACCACGGAGGCGCCA+GGG | 0.609462 | 5.3:+7131664 | MS.gene21491:CDS |
| CAGAAAACAACGCGAAACTG+CGG | 0.621911 | 5.3:-7131858 | None:intergenic |
| GCCAGGGATGAACGGTGCAG+TGG | 0.624288 | 5.3:+7131680 | MS.gene21491:CDS |
| CGTGGTTGGTTTCACCGCCG+AGG | 0.625680 | 5.3:-7131652 | None:intergenic |
| AAAGAATGGCGGTGCCTCGG+CGG | 0.629398 | 5.3:+7131638 | MS.gene21491:CDS |
| GCATAAGAATGGGTTTACTG+TGG | 0.664009 | 5.3:+7131556 | None:intergenic |
| TCGTTGGGAAATTCTGCCGG+CGG | 0.672246 | 5.3:+7131821 | MS.gene21491:CDS |
| TGAAGCGAAGTCATCGACGA+CGG | 0.700439 | 5.3:+7131605 | MS.gene21491:CDS |
| GAGAACGCAGCAAAGAATGG+CGG | 0.729371 | 5.3:+7131627 | MS.gene21491:CDS |
| CTCGGCGGTGAAACCAACCA+CGG | 0.734856 | 5.3:+7131653 | MS.gene21491:CDS |
| GGCGGTGAAACCAACCACGG+AGG | 0.756389 | 5.3:+7131656 | MS.gene21491:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTGTTTCAGTTTTCGAGTT+TGG | + | chr5.3:7131726-7131745 | MS.gene21491:CDS | 35.0% |
| !!! | TTCAGTTTTCGAGTTTGGTT+CGG | + | chr5.3:7131731-7131750 | MS.gene21491:CDS | 35.0% |
| AACGATAAGGTTACTCTTGC+TGG | + | chr5.3:7131765-7131784 | MS.gene21491:CDS | 40.0% | |
| AAGATCTTGAACCTTGTCGT+TGG | + | chr5.3:7131805-7131824 | MS.gene21491:CDS | 40.0% | |
| AGATCTTGAACCTTGTCGTT+GGG | + | chr5.3:7131806-7131825 | MS.gene21491:CDS | 40.0% | |
| GGTTCAAGATCTTCAGAAAC+TGG | - | chr5.3:7131798-7131817 | None:intergenic | 40.0% | |
| ACTGAAACACTCGCGTTCAA+AGG | - | chr5.3:7131717-7131736 | None:intergenic | 45.0% | |
| CAGAAAACAACGCGAAACTG+CGG | - | chr5.3:7131861-7131880 | None:intergenic | 45.0% | |
| TTGTCGTTGGGAAATTCTGC+CGG | + | chr5.3:7131818-7131837 | MS.gene21491:CDS | 45.0% | |
| ACGGAGAACGCAGCAAAGAA+TGG | + | chr5.3:7131624-7131643 | MS.gene21491:CDS | 50.0% | |
| ACGGTGCAGTGGAGGTTAAA+CGG | + | chr5.3:7131691-7131710 | MS.gene21491:CDS | 50.0% | |
| GAGAACGCAGCAAAGAATGG+CGG | + | chr5.3:7131627-7131646 | MS.gene21491:CDS | 50.0% | |
| GGCAGAATTTCCCAACGACA+AGG | - | chr5.3:7131819-7131838 | None:intergenic | 50.0% | |
| TGAAGCGAAGTCATCGACGA+CGG | + | chr5.3:7131605-7131624 | MS.gene21491:CDS | 50.0% | |
| AGCAAAGAATGGCGGTGCCT+CGG | + | chr5.3:7131635-7131654 | MS.gene21491:CDS | 55.0% | |
| GGTTGCTGCGAGCAACGATA+AGG | + | chr5.3:7131752-7131771 | MS.gene21491:CDS | 55.0% | |
| GTCGTCGATGACTTCGCTTC+AGG | - | chr5.3:7131606-7131625 | None:intergenic | 55.0% | |
| TCGTTGGGAAATTCTGCCGG+CGG | + | chr5.3:7131821-7131840 | MS.gene21491:CDS | 55.0% | |
| !! | GGTTTACTGTGGTGGCGGAA+GGG | + | chr5.3:7131567-7131586 | MS.gene21491:CDS | 55.0% |
| !! | GTTTACTGTGGTGGCGGAAG+GGG | + | chr5.3:7131568-7131587 | MS.gene21491:CDS | 55.0% |
| AAAGAATGGCGGTGCCTCGG+CGG | + | chr5.3:7131638-7131657 | MS.gene21491:CDS | 60.0% | |
| AGGGATGAACGGTGCAGTGG+AGG | + | chr5.3:7131683-7131702 | MS.gene21491:CDS | 60.0% | |
| CTCGGCGGTGAAACCAACCA+CGG | + | chr5.3:7131653-7131672 | MS.gene21491:CDS | 60.0% | |
| TCCACTGCACCGTTCATCCC+TGG | - | chr5.3:7131684-7131703 | None:intergenic | 60.0% | |
| !! | GGGTTTACTGTGGTGGCGGA+AGG | + | chr5.3:7131566-7131585 | MS.gene21491:CDS | 60.0% |
| GCCAGGGATGAACGGTGCAG+TGG | + | chr5.3:7131680-7131699 | MS.gene21491:CDS | 65.0% | |
| GGCGCGTTTGACTCAACCGC+CGG | - | chr5.3:7131840-7131859 | None:intergenic | 65.0% | |
| GGCGGTGAAACCAACCACGG+AGG | + | chr5.3:7131656-7131675 | MS.gene21491:CDS | 65.0% | |
| !! | AAACCAACCACGGAGGCGCC+AGG | + | chr5.3:7131663-7131682 | MS.gene21491:CDS | 65.0% |
| !! | AACCAACCACGGAGGCGCCA+GGG | + | chr5.3:7131664-7131683 | MS.gene21491:CDS | 65.0% |
| !! | ACGGAGGCGCCAGGGATGAA+CGG | + | chr5.3:7131672-7131691 | MS.gene21491:CDS | 65.0% |
| !! | CGTGGTTGGTTTCACCGCCG+AGG | - | chr5.3:7131655-7131674 | None:intergenic | 65.0% |
| ATCCCTGGCGCCTCCGTGGT+TGG | - | chr5.3:7131669-7131688 | None:intergenic | 70.0% | |
| GTTCATCCCTGGCGCCTCCG+TGG | - | chr5.3:7131673-7131692 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 7131564 | 7131881 | 7131564 | ID=MS.gene21491 |
| chr5.3 | mRNA | 7131564 | 7131881 | 7131564 | ID=MS.gene21491.t1;Parent=MS.gene21491 |
| chr5.3 | exon | 7131564 | 7131881 | 7131564 | ID=MS.gene21491.t1.exon1;Parent=MS.gene21491.t1 |
| chr5.3 | CDS | 7131564 | 7131881 | 7131564 | ID=cds.MS.gene21491.t1;Parent=MS.gene21491.t1 |
| Gene Sequence |
| Protein sequence |
