AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22186

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22186.t1 MTR_3g046300 95.874 412 15 1 1 412 52 461 0.0 814
MS.gene22186.t1 MTR_5g091130 81.643 414 65 5 3 412 150 556 0.0 668
MS.gene22186.t1 MTR_1g111350 39.332 389 196 10 29 409 111 467 8.45e-77 246
MS.gene22186.t1 MTR_3g097150 38.519 405 208 12 16 412 78 449 1.22e-75 242
MS.gene22186.t1 MTR_7g117430 39.589 389 199 9 31 410 124 485 1.57e-75 243
MS.gene22186.t1 MTR_1g115210 33.738 412 232 8 6 409 108 486 9.90e-70 228
MS.gene22186.t1 MTR_4g007740 33.943 383 229 10 31 409 115 477 5.35e-65 215
MS.gene22186.t1 MTR_1g039270 34.921 378 210 7 40 409 120 469 1.47e-63 211
MS.gene22186.t1 MTR_8g103880 31.266 403 241 8 15 410 83 456 2.21e-61 205
MS.gene22186.t1 MTR_3g053710 35.040 371 219 9 44 409 113 466 1.54e-60 203
MS.gene22186.t1 MTR_2g028190 33.158 380 228 11 38 410 92 452 6.28e-60 201
MS.gene22186.t1 MTR_6g085010 36.188 362 205 8 48 409 156 491 7.14e-59 199
MS.gene22186.t1 MTR_5g038360 33.791 364 224 9 49 411 89 436 8.62e-58 195
MS.gene22186.t1 MTR_0036s0110 33.773 379 220 12 39 409 85 440 1.71e-57 195
MS.gene22186.t1 MTR_0036s0070 32.902 386 220 11 33 412 75 427 2.37e-57 194
MS.gene22186.t1 MTR_8g096990 32.292 384 240 9 30 412 68 432 2.94e-56 191
MS.gene22186.t1 MTR_5g044770 32.552 384 238 9 30 412 68 431 4.66e-56 191
MS.gene22186.t1 MTR_3g053980 36.096 374 215 9 39 409 133 485 1.63e-54 188
MS.gene22186.t1 MTR_8g038280 33.693 371 222 11 47 412 85 436 2.80e-54 186
MS.gene22186.t1 MTR_7g105850 32.178 404 210 14 47 411 89 467 3.00e-54 187
MS.gene22186.t1 MTR_2g059460 34.211 380 222 15 36 409 47 404 2.15e-53 183
MS.gene22186.t1 MTR_5g080890 33.773 379 231 11 36 412 74 434 3.12e-53 183
MS.gene22186.t1 MTR_5g080960 33.509 379 232 11 36 412 74 434 8.10e-52 179
MS.gene22186.t1 MTR_5g008150 32.162 370 227 10 47 411 62 412 2.71e-49 172
MS.gene22186.t1 MTR_3g028120 31.436 369 236 10 46 412 86 439 2.18e-48 171
MS.gene22186.t1 MTR_6g090290 32.518 409 253 10 8 411 46 436 6.09e-48 169
MS.gene22186.t1 MTR_2g059490 31.842 380 240 12 37 412 47 411 1.55e-47 168
MS.gene22186.t1 MTR_0036s0050 30.570 386 236 10 33 411 75 435 2.33e-47 168
MS.gene22186.t1 MTR_5g008110 31.367 373 225 13 47 411 66 415 8.56e-47 166
MS.gene22186.t1 MTR_1g037630 34.146 369 208 10 46 409 90 428 1.24e-45 163
MS.gene22186.t1 MTR_5g044570 31.720 372 207 10 43 411 84 411 1.93e-45 162
MS.gene22186.t1 MTR_8g038310 30.713 407 243 11 6 411 45 413 3.56e-45 161
MS.gene22186.t1 MTR_8g038250 30.412 388 226 9 30 412 68 416 3.63e-45 161
MS.gene22186.t1 MTR_3g014290 33.875 369 209 10 46 409 78 416 6.55e-45 160
MS.gene22186.t1 MTR_2g067830 31.139 395 237 15 25 409 35 404 9.25e-45 160
MS.gene22186.t1 MTR_0024s0040 32.609 368 227 11 47 411 61 410 2.55e-44 159
MS.gene22186.t1 MTR_8g039540 32.824 393 227 12 24 409 71 433 1.69e-42 155
MS.gene22186.t1 MTR_2g105300 30.919 359 204 16 63 406 50 379 3.43e-41 150
MS.gene22186.t1 MTR_4g045857 37.549 253 145 7 37 287 47 288 1.74e-40 145
MS.gene22186.t1 MTR_1g060750 29.268 369 213 11 46 409 49 374 1.21e-39 145
MS.gene22186.t1 MTR_5g008070 30.079 379 225 13 42 409 75 424 5.56e-39 145
MS.gene22186.t1 MTR_4g056420 30.077 389 220 16 46 409 75 436 1.99e-38 144
MS.gene22186.t1 MTR_2g063110 29.524 420 231 13 37 409 107 508 1.14e-37 143
MS.gene22186.t1 MTR_2g084550 29.921 381 236 13 44 409 73 437 1.26e-37 142
MS.gene22186.t1 MTR_6g088760 32.641 337 198 14 46 373 86 402 6.52e-37 141
MS.gene22186.t1 MTR_4g109760 32.861 353 212 12 63 412 69 399 7.01e-37 139
MS.gene22186.t1 MTR_7g062360 29.367 395 218 15 51 412 62 428 1.56e-35 135
MS.gene22186.t1 MTR_4g100990 28.710 411 230 18 48 409 74 470 4.81e-35 135
MS.gene22186.t1 MTR_7g102750 28.647 377 239 13 47 409 80 440 3.93e-34 133
MS.gene22186.t1 MTR_3g100500 30.709 381 231 10 36 410 85 438 6.34e-34 131
MS.gene22186.t1 MTR_5g012490 26.570 414 234 15 48 409 74 469 5.64e-33 129
MS.gene22186.t1 MTR_2g055840 27.807 374 243 9 49 409 14 373 1.30e-32 126
MS.gene22186.t1 MTR_2g070430 28.238 386 232 12 46 406 85 450 5.06e-32 126
MS.gene22186.t1 MTR_3g068000 26.992 389 233 15 51 410 76 442 5.82e-32 126
MS.gene22186.t1 MTR_1g061940 27.532 385 243 13 43 409 78 444 1.43e-31 125
MS.gene22186.t1 MTR_4g095270 25.323 387 231 12 46 412 78 426 3.49e-31 125
MS.gene22186.t1 MTR_7g102750 29.969 327 201 12 47 361 80 390 4.56e-31 123
MS.gene22186.t1 MTR_4g095270 25.323 387 231 12 46 412 78 426 8.58e-31 124
MS.gene22186.t1 MTR_3g065160 27.273 396 221 15 46 412 72 429 1.56e-29 119
MS.gene22186.t1 MTR_5g077850 27.749 382 223 15 55 412 88 440 3.06e-29 118
MS.gene22186.t1 MTR_5g022880 28.682 387 212 17 49 409 113 461 3.79e-29 119
MS.gene22186.t1 MTR_1g078240 29.925 401 219 20 40 409 61 430 4.99e-29 118
MS.gene22186.t1 MTR_5g016260 25.330 379 241 12 46 412 86 434 5.54e-29 119
MS.gene22186.t1 MTR_5g016260 25.330 379 241 12 46 412 86 434 1.28e-28 118
MS.gene22186.t1 MTR_1g078240 29.925 401 218 21 40 409 61 429 5.11e-28 115
MS.gene22186.t1 MTR_1g078240 33.228 316 164 17 40 332 61 352 3.78e-26 108
MS.gene22186.t1 MTR_7g080230 28.750 400 223 19 40 409 63 430 4.21e-26 110
MS.gene22186.t1 MTR_8g075090 29.016 386 214 16 49 411 113 461 1.20e-25 109
MS.gene22186.t1 MTR_8g075090 28.682 387 216 16 49 412 113 462 2.46e-25 107
MS.gene22186.t1 MTR_1g093100 26.396 394 219 15 57 407 55 420 9.42e-25 105
MS.gene22186.t1 MTR_7g021510 24.409 381 252 13 47 409 71 433 1.01e-24 106
MS.gene22186.t1 MTR_5g061690 27.820 399 240 19 30 411 84 451 3.61e-24 104
MS.gene22186.t1 MTR_6g087710 59.756 82 28 1 336 412 1 82 4.13e-24 95.5
MS.gene22186.t1 MTR_1g072380 23.515 404 236 13 48 405 56 432 1.11e-23 102
MS.gene22186.t1 MTR_1g072450 24.485 437 250 18 8 407 24 417 3.09e-23 101
MS.gene22186.t1 MTR_8g075010 24.880 418 237 18 33 412 74 452 1.73e-22 99.8
MS.gene22186.t1 MTR_3g087780 28.148 405 236 19 30 412 82 453 2.18e-22 99.8
MS.gene22186.t1 MTR_1g072420 23.744 438 253 16 8 407 24 418 3.88e-22 98.2
MS.gene22186.t1 MTR_8g075090 29.526 359 193 16 49 384 113 434 7.38e-22 97.8
MS.gene22186.t1 MTR_4g023650 24.146 410 249 15 32 412 46 422 1.61e-21 96.3
MS.gene22186.t1 MTR_5g077920 26.142 394 214 14 55 411 75 428 2.40e-20 92.8
MS.gene22186.t1 MTR_6g084710 26.065 399 235 17 40 409 183 550 2.69e-20 93.6
MS.gene22186.t1 MTR_3g096930 25.065 387 237 15 47 412 83 437 2.94e-20 92.8
MS.gene22186.t1 MTR_5g008090 26.935 323 212 11 94 411 11 314 5.53e-20 90.5
MS.gene22186.t1 MTR_2g022490 25.130 386 226 13 49 396 44 404 7.32e-20 91.3
MS.gene22186.t1 MTR_4g023630 24.257 404 256 16 32 412 46 422 9.08e-20 91.3
MS.gene22186.t1 MTR_1g029810 25.065 387 239 17 47 412 56 412 3.94e-19 89.0
MS.gene22186.t1 MTR_1g029810 25.065 387 239 17 47 412 56 412 6.22e-19 88.6
MS.gene22186.t1 MTR_2g022450 23.291 395 251 14 44 405 36 411 9.82e-19 88.2
MS.gene22186.t1 MTR_2g022380 25.063 395 225 15 53 405 44 409 1.95e-18 87.4
MS.gene22186.t1 MTR_2g022430 24.390 410 222 18 49 407 40 412 8.13e-18 85.1
MS.gene22186.t1 MTR_2g022460 28.195 266 165 11 156 405 139 394 8.30e-18 85.1
MS.gene22186.t1 MTR_5g061690 26.510 298 189 14 121 411 38 312 1.11e-17 84.3
MS.gene22186.t1 MTR_2g022350 25.000 396 224 16 53 405 44 409 2.08e-17 84.0
MS.gene22186.t1 MTR_6g084710 27.586 290 173 12 40 319 183 445 4.39e-17 83.6
MS.gene22186.t1 MTR_2g022480 23.575 386 232 13 49 396 50 410 2.34e-16 80.9
MS.gene22186.t1 MTR_7g113790 24.000 400 254 19 46 412 48 430 3.57e-16 80.5
MS.gene22186.t1 MTR_4g117090 22.906 406 249 16 48 405 49 438 9.55e-16 79.3
MS.gene22186.t1 MTR_7g080230 27.778 360 198 19 80 409 33 360 1.25e-15 78.6
MS.gene22186.t1 MTR_2g022320 23.116 398 236 12 49 405 37 405 2.60e-15 77.8
MS.gene22186.t1 MTR_2g022370 24.021 383 240 14 53 405 44 405 2.67e-15 77.8
MS.gene22186.t1 MTR_3g087780 26.176 340 208 15 85 412 3 311 1.61e-14 75.1
MS.gene22186.t1 MTR_3g087780 26.346 353 214 16 75 412 26 347 1.62e-14 75.1
MS.gene22186.t1 MTR_0252s0030 26.351 148 105 3 228 373 182 327 2.30e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22186.t1 AT3G59080 68.434 415 119 5 2 412 129 535 0.0 565
MS.gene22186.t1 AT2G42980 67.146 417 125 6 2 412 117 527 0.0 546
MS.gene22186.t1 AT3G59080 62.651 415 107 5 2 412 129 499 1.07e-173 495
MS.gene22186.t1 AT3G25700 40.731 383 206 12 40 409 75 449 4.04e-82 259
MS.gene22186.t1 AT1G01300 40.519 385 189 10 36 409 129 484 3.11e-76 245
MS.gene22186.t1 AT1G25510 40.420 381 188 8 36 409 135 483 3.58e-74 239
MS.gene22186.t1 AT3G61820 37.767 421 226 8 1 409 87 483 1.07e-73 238
MS.gene22186.t1 AT2G03200 35.733 389 199 11 45 412 103 461 1.17e-70 230
MS.gene22186.t1 AT3G18490 38.860 386 205 11 29 409 141 500 6.99e-70 229
MS.gene22186.t1 AT5G10770 36.761 389 219 11 24 409 109 473 1.51e-66 219
MS.gene22186.t1 AT3G20015 33.947 380 214 6 38 409 120 470 2.77e-62 208
MS.gene22186.t1 AT1G79720 34.474 380 220 10 38 409 124 482 6.14e-58 197
MS.gene22186.t1 AT5G33340 35.598 368 219 8 46 412 87 437 2.70e-57 194
MS.gene22186.t1 AT2G35615 35.294 391 230 13 30 409 65 443 3.09e-56 191
MS.gene22186.t1 AT1G64830 34.133 375 227 11 39 412 76 431 2.01e-53 184
MS.gene22186.t1 AT3G12700 31.920 401 227 13 29 409 86 460 4.60e-53 184
MS.gene22186.t1 AT1G31450 34.908 381 226 12 38 409 74 441 5.81e-53 183
MS.gene22186.t1 AT3G54400 30.905 398 222 12 30 409 62 424 9.09e-50 174
MS.gene22186.t1 AT5G10760 34.474 380 217 11 31 409 116 464 9.55e-49 172
MS.gene22186.t1 AT3G52500 30.717 446 237 19 5 409 53 467 1.01e-48 172
MS.gene22186.t1 AT2G28010 29.650 371 223 9 44 412 60 394 5.66e-46 163
MS.gene22186.t1 AT2G28040 29.793 386 229 9 30 412 38 384 2.64e-45 161
MS.gene22186.t1 AT1G09750 31.429 385 224 12 38 410 93 449 6.10e-45 162
MS.gene22186.t1 AT2G28040 29.793 386 229 9 30 412 47 393 6.65e-45 160
MS.gene22186.t1 AT4G30030 30.769 377 220 13 47 412 76 422 1.28e-44 160
MS.gene22186.t1 AT4G30040 30.807 409 245 12 11 404 42 427 3.24e-43 156
MS.gene22186.t1 AT5G07030 32.891 377 208 13 46 410 112 455 3.47e-43 157
MS.gene22186.t1 AT2G28030 28.767 365 224 8 49 412 61 390 1.03e-39 146
MS.gene22186.t1 AT2G28225 27.371 369 228 7 49 412 67 400 6.25e-37 139
MS.gene22186.t1 AT5G22850 29.275 386 232 15 44 409 76 440 2.40e-36 139
MS.gene22186.t1 AT2G28220 28.495 372 220 10 49 412 67 400 4.50e-36 137
MS.gene22186.t1 AT5G02190 27.604 384 225 15 58 410 82 443 4.04e-33 129
MS.gene22186.t1 AT2G36670 27.632 380 238 13 47 409 98 457 1.36e-32 129
MS.gene22186.t1 AT1G08210 28.792 389 227 15 44 409 79 440 1.49e-32 128
MS.gene22186.t1 AT5G36260 27.034 381 237 15 47 409 76 433 1.54e-32 128
MS.gene22186.t1 AT1G08210 28.792 389 227 15 44 409 113 474 2.35e-32 128
MS.gene22186.t1 AT1G08210 28.792 389 227 15 44 409 127 488 2.53e-32 128
MS.gene22186.t1 AT2G23945 28.947 380 230 14 49 412 96 451 3.40e-32 127
MS.gene22186.t1 AT2G36670 27.513 378 237 13 49 409 105 462 4.21e-32 127
MS.gene22186.t1 AT2G36670 27.513 378 237 13 49 409 34 391 5.53e-32 126
MS.gene22186.t1 AT2G36670 27.513 378 237 13 49 409 34 391 5.53e-32 126
MS.gene22186.t1 AT4G12920 28.962 366 221 14 49 410 58 388 1.49e-31 124
MS.gene22186.t1 AT5G43100 26.509 381 237 14 44 412 71 420 5.36e-31 124
MS.gene22186.t1 AT5G45120 28.537 410 230 19 49 409 83 478 1.42e-30 123
MS.gene22186.t1 AT2G39710 26.463 393 229 19 51 410 67 432 1.96e-30 122
MS.gene22186.t1 AT5G43100 26.509 381 237 14 44 412 71 420 2.11e-30 124
MS.gene22186.t1 AT1G65240 25.722 381 239 13 47 409 72 426 7.52e-30 120
MS.gene22186.t1 AT4G33490 28.426 394 217 18 47 412 58 414 8.22e-30 120
MS.gene22186.t1 AT5G37540 26.402 428 257 18 15 409 36 438 1.74e-29 119
MS.gene22186.t1 AT1G66180 28.109 402 213 18 46 412 69 429 3.12e-29 118
MS.gene22186.t1 AT1G05840 27.390 387 231 15 47 409 78 438 3.30e-29 119
MS.gene22186.t1 AT3G02740 25.916 382 239 16 47 409 83 439 2.45e-28 116
MS.gene22186.t1 AT3G02740 25.840 387 243 16 42 409 3 364 2.76e-28 115
MS.gene22186.t1 AT1G77480 27.089 395 236 15 40 412 58 422 2.83e-28 115
MS.gene22186.t1 AT1G77480 27.848 395 233 16 40 412 58 422 3.04e-28 116
MS.gene22186.t1 AT1G08210 27.427 412 230 17 44 409 79 467 1.75e-27 114
MS.gene22186.t1 AT3G50050 24.227 388 235 14 46 412 90 439 2.75e-25 108
MS.gene22186.t1 AT3G25700 41.606 137 74 4 40 174 75 207 2.09e-23 100
MS.gene22186.t1 AT3G25700 42.105 133 71 5 281 409 217 347 1.75e-22 98.2
MS.gene22186.t1 AT3G51350 26.234 385 230 15 49 411 102 454 4.95e-23 101
MS.gene22186.t1 AT2G17760 26.649 379 227 17 49 409 104 449 2.10e-22 99.8
MS.gene22186.t1 AT1G03230 25.385 390 223 16 57 405 53 415 4.35e-22 98.2
MS.gene22186.t1 AT4G35880 26.396 394 214 16 49 412 107 454 4.62e-22 98.6
MS.gene22186.t1 AT4G33490 28.125 352 191 17 47 373 55 369 7.30e-22 97.1
MS.gene22186.t1 AT1G44130 25.441 397 238 18 40 412 40 402 3.53e-20 92.0
MS.gene22186.t1 AT3G51360 25.459 381 234 15 49 411 5 353 1.43e-19 90.5
MS.gene22186.t1 AT3G51360 25.063 399 241 16 39 411 81 447 1.94e-19 90.5
MS.gene22186.t1 AT3G51360 25.063 399 241 16 39 411 71 437 2.48e-19 90.1
MS.gene22186.t1 AT5G10080 26.434 401 235 17 37 411 89 455 4.58e-19 89.7
MS.gene22186.t1 AT4G35880 27.248 367 191 16 49 385 107 427 7.37e-19 88.6
MS.gene22186.t1 AT4G16563 26.764 411 215 17 66 409 100 491 3.44e-18 87.0
MS.gene22186.t1 AT1G49050 22.785 395 258 16 41 409 195 568 3.60e-18 87.0
MS.gene22186.t1 AT1G49050 22.739 387 252 16 49 409 30 395 4.83e-18 85.9
MS.gene22186.t1 AT3G51330 24.869 382 240 13 49 411 102 455 5.33e-16 80.1
MS.gene22186.t1 AT3G51330 24.737 380 239 13 49 409 102 453 5.45e-16 80.1
MS.gene22186.t1 AT3G51360 25.000 388 226 17 39 393 81 436 4.24e-15 77.0
MS.gene22186.t1 AT3G51360 25.000 388 226 17 39 393 71 426 4.43e-15 77.0
MS.gene22186.t1 AT1G49050 24.074 270 176 11 41 301 195 444 9.83e-15 76.3
MS.gene22186.t1 AT3G42550 38.776 98 56 1 46 143 75 168 1.07e-14 75.9
MS.gene22186.t1 AT3G51330 25.000 356 220 13 49 385 102 429 8.09e-13 70.1
MS.gene22186.t1 AT3G51360 23.939 330 210 13 91 411 14 311 1.39e-12 68.9
MS.gene22186.t1 AT3G51360 23.939 330 210 13 91 411 14 311 1.39e-12 68.9
MS.gene22186.t1 AT3G12700 32.673 101 59 2 29 121 86 185 2.03e-12 67.4
MS.gene22186.t1 AT3G12700 32.673 101 59 2 29 121 86 185 2.20e-12 67.0
MS.gene22186.t1 AT5G24820 23.958 384 237 14 50 409 47 399 7.51e-12 67.0
MS.gene22186.t1 AT3G51360 24.556 338 199 15 39 352 71 376 8.97e-12 66.6
MS.gene22186.t1 AT3G51360 24.556 338 199 15 39 352 81 386 9.45e-12 66.6

Find 71 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTATAACATGGCTTTAA+TGG 0.170426 3.4:-40855581 None:intergenic
AAAGATGTGAAATTCAAATT+AGG 0.176046 3.4:-40855326 None:intergenic
AAGTCTGCACTTTCAATAAT+TGG 0.182806 3.4:+40855743 MS.gene22186:CDS
GAAATGGAATTGCCTGAATT+TGG 0.207197 3.4:+40855617 MS.gene22186:CDS
AGCATTCATGAAGAAAATTA+AGG 0.230380 3.4:+40855532 MS.gene22186:CDS
TTAAAAGAAGTGGAATCTTT+AGG 0.256516 3.4:-40854891 None:intergenic
GTTAGTAGCAAATTGTTATT+TGG 0.258060 3.4:+40855278 MS.gene22186:CDS
TTTAATTTCCAAGTTTCTTC+TGG 0.286628 3.4:-40855425 None:intergenic
ACACCGGTAGTGATCTTAAC+TGG 0.316215 3.4:+40854814 MS.gene22186:CDS
GGTGGTACAATAATTGATTC+TGG 0.324176 3.4:+40855461 MS.gene22186:CDS
AAGATGTGAAATTCAAATTA+GGG 0.331531 3.4:-40855325 None:intergenic
TAAACTTCATCACACTTCAT+TGG 0.337151 3.4:-40855830 None:intergenic
CGCGGCGCTTCCGGTTTGTT+AGG 0.341387 3.4:+40855158 MS.gene22186:CDS
ATCTGATGATGTTGTTTGTT+TGG 0.342174 3.4:+40855703 MS.gene22186:CDS
AAACTATTCAACTCAGCTTA+TGG 0.349947 3.4:+40854698 MS.gene22186:CDS
AGCAACAGCCGGCGGCTTAT+AGG 0.352574 3.4:-40854646 None:intergenic
TTGGAATCAGGAGTCAGTTT+TGG 0.353601 3.4:+40854726 MS.gene22186:CDS
AAGCTGAGTTGAATAGTTTC+CGG 0.354869 3.4:-40854694 None:intergenic
ATCGTCGAAAATGTTATGTT+TGG 0.362495 3.4:+40855107 MS.gene22186:CDS
GAAATAAAATCTATTATAGT+TGG 0.366850 3.4:+40855383 MS.gene22186:CDS
TTGAACACGGATTTCCCATT+TGG 0.368704 3.4:-40855083 None:intergenic
TAGTTTCCGGTGAAATATTC+CGG 0.373975 3.4:-40854681 None:intergenic
GAAAACAATATTCCAAATTC+AGG 0.374885 3.4:-40855629 None:intergenic
GGACTATTTCGCGGCGCTTC+CGG 0.376570 3.4:+40855149 MS.gene22186:CDS
TCACAGCTTCAAAATCTCTA+TGG 0.389061 3.4:+40855209 MS.gene22186:CDS
TTATGTTTGGTTGTGGTCAT+TGG 0.389748 3.4:+40855120 MS.gene22186:CDS
CTTATGGCCACCTTGGAATC+AGG 0.409948 3.4:+40854714 MS.gene22186:CDS
ATTTCTATCCACAAGGCAAT+AGG 0.414216 3.4:-40855243 None:intergenic
TGAATAAAATAATTCTCAAC+AGG 0.421496 3.4:-40855674 None:intergenic
GTGAATCTTACAAAACCAAA+TGG 0.427826 3.4:+40855068 MS.gene22186:CDS
AAATTAAATGAAGATGGTTC+TGG 0.430404 3.4:+40855440 MS.gene22186:CDS
GAATCTTTAGGATCAAAATA+TGG 0.439973 3.4:-40854879 None:intergenic
TGATCCTCCTCAGCCTTGTA+AGG 0.451019 3.4:+40854959 MS.gene22186:CDS
TTACAAGGCTGAGGAGGATC+AGG 0.451589 3.4:-40854957 None:intergenic
GCTTCCGGTTTGTTAGGATT+AGG 0.454470 3.4:+40855164 MS.gene22186:CDS
TGATTCTCCTCAGCCTTACA+AGG 0.456690 3.4:-40854972 None:intergenic
GGTTGTGGTCATTGGAATAG+AGG 0.457397 3.4:+40855128 MS.gene22186:CDS
ATTATTGAAAGTGCAGACTT+AGG 0.458899 3.4:-40855740 None:intergenic
GCATTCATGAAGAAAATTAA+GGG 0.463799 3.4:+40855533 MS.gene22186:CDS
AACTCAGCTTATGGCCACCT+TGG 0.470142 3.4:+40854707 MS.gene22186:CDS
ACTTGGAAATTAAATGAAGA+TGG 0.473531 3.4:+40855434 MS.gene22186:CDS
TGTGAGTGGAATTGAAGAAA+TGG 0.475079 3.4:+40855601 MS.gene22186:CDS
TCTTCCTAATCCTAACAAAC+CGG 0.491064 3.4:-40855168 None:intergenic
ACTTATGTTCTTAAAAGAAG+TGG 0.498876 3.4:-40854901 None:intergenic
GGTGATAGTTCTAACACAAC+TGG 0.500373 3.4:+40855020 MS.gene22186:CDS
TGTTGCTCTCGCAGCATCAC+CGG 0.501414 3.4:+40854662 MS.gene22186:CDS
GAAAATGTTATGTTTGGTTG+TGG 0.526829 3.4:+40855113 MS.gene22186:CDS
ATAAAATCTATTATAGTTGG+TGG 0.527088 3.4:+40855386 MS.gene22186:CDS
TGAATCTTACAAAACCAAAT+GGG 0.529298 3.4:+40855069 MS.gene22186:CDS
TATTTCATCGATGTATTCAT+CGG 0.530587 3.4:+40854759 MS.gene22186:CDS
TCCTCAGCCTTGTAAGGCTG+AGG 0.538561 3.4:+40854965 MS.gene22186:CDS
AGCTGTGAAGCAAATGATAA+TGG 0.540080 3.4:-40855194 None:intergenic
TCTTGAATAATCTCATAAGC+TGG 0.545661 3.4:-40855509 None:intergenic
TCCTCAGCCTTACAAGGCTG+AGG 0.548640 3.4:-40854966 None:intergenic
AAAACTGACTCCTGATTCCA+AGG 0.558968 3.4:-40854724 None:intergenic
GATGCTGCGAGAGCAACAGC+CGG 0.577927 3.4:-40854657 None:intergenic
TGGAATAGAGGACTATTTCG+CGG 0.580688 3.4:+40855140 MS.gene22186:CDS
AATCCAGTTAAGATCACTAC+CGG 0.581468 3.4:-40854817 None:intergenic
AAGCCATGTTATAATGTGAG+TGG 0.592391 3.4:+40855587 MS.gene22186:CDS
GGTTTGTTAGGATTAGGAAG+AGG 0.594942 3.4:+40855170 MS.gene22186:CDS
GCTGCGAGAGCAACAGCCGG+CGG 0.598841 3.4:-40854654 None:intergenic
TCAGCCTTACAAGGCTGAGG+AGG 0.598858 3.4:-40854963 None:intergenic
AATTGCTATTTCTATCCACA+AGG 0.603446 3.4:-40855250 None:intergenic
GCATCACCGGAATATTTCAC+CGG 0.610906 3.4:+40854675 MS.gene22186:CDS
AAAGAAGTCCTATAAGCCGC+CGG 0.612023 3.4:+40854638 MS.gene22186:CDS
TTAAATGAAGATGGTTCTGG+TGG 0.619428 3.4:+40855443 MS.gene22186:CDS
ATTCCACTCACATTATAACA+TGG 0.620105 3.4:-40855590 None:intergenic
TGATCAAAACAAGCATAACA+AGG 0.620119 3.4:-40854849 None:intergenic
TACATCGATGAAATACTCAC+CGG 0.634881 3.4:-40854751 None:intergenic
GATGTGAAGAAATCTAGACT+TGG 0.686532 3.4:+40855800 MS.gene22186:CDS
ACTGACTCCTGATTCCAAGG+TGG 0.742727 3.4:-40854721 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAAAATCTATTATAGT+TGG + chr3.4:40855383-40855402 MS.gene22186:CDS 15.0%
!! AAAGATGTGAAATTCAAATT+AGG - chr3.4:40855329-40855348 None:intergenic 20.0%
!! AAGATGTGAAATTCAAATTA+GGG - chr3.4:40855328-40855347 None:intergenic 20.0%
!! ATAAAATCTATTATAGTTGG+TGG + chr3.4:40855386-40855405 MS.gene22186:CDS 20.0%
!! TGAATAAAATAATTCTCAAC+AGG - chr3.4:40855677-40855696 None:intergenic 20.0%
! AAATTAAATGAAGATGGTTC+TGG + chr3.4:40855440-40855459 MS.gene22186:CDS 25.0%
! ACTTATGTTCTTAAAAGAAG+TGG - chr3.4:40854904-40854923 None:intergenic 25.0%
! ACTTGGAAATTAAATGAAGA+TGG + chr3.4:40855434-40855453 MS.gene22186:CDS 25.0%
! AGCATTCATGAAGAAAATTA+AGG + chr3.4:40855532-40855551 MS.gene22186:CDS 25.0%
! GAAAACAATATTCCAAATTC+AGG - chr3.4:40855632-40855651 None:intergenic 25.0%
! GCATTCATGAAGAAAATTAA+GGG + chr3.4:40855533-40855552 MS.gene22186:CDS 25.0%
! TAAAAATACCAGAAGAAACT+TGG + chr3.4:40855417-40855436 MS.gene22186:CDS 25.0%
! TATTTCATCGATGTATTCAT+CGG + chr3.4:40854759-40854778 MS.gene22186:CDS 25.0%
! TGAATCTTACAAAACCAAAT+GGG + chr3.4:40855069-40855088 MS.gene22186:CDS 25.0%
! TTAAAAGAAGTGGAATCTTT+AGG - chr3.4:40854894-40854913 None:intergenic 25.0%
!! AGTATCAGAGAAAAATGTTT+TGG - chr3.4:40854792-40854811 None:intergenic 25.0%
!! GTTAGTAGCAAATTGTTATT+TGG + chr3.4:40855278-40855297 MS.gene22186:CDS 25.0%
!! TTGAATTTCACATCTTTTGT+TGG + chr3.4:40855332-40855351 MS.gene22186:CDS 25.0%
!! TTTAATTTCCAAGTTTCTTC+TGG - chr3.4:40855428-40855447 None:intergenic 25.0%
!!! AAACTTGTCCGTATTTTTAT+TGG + chr3.4:40854994-40855013 MS.gene22186:CDS 25.0%
!!! ACATTATAACATGGCTTTAA+TGG - chr3.4:40855584-40855603 None:intergenic 25.0%
!!! GAATCTTTAGGATCAAAATA+TGG - chr3.4:40854882-40854901 None:intergenic 25.0%
!!! TATGCTTGTTTTGATCAAAA+TGG + chr3.4:40854855-40854874 MS.gene22186:CDS 25.0%
!!! TTGTTTGTTTGGCTATTTTA+GGG + chr3.4:40855714-40855733 MS.gene22186:CDS 25.0%
!!! TTTGGAATATTGTTTTCAGA+TGG + chr3.4:40855635-40855654 MS.gene22186:CDS 25.0%
AAACTATTCAACTCAGCTTA+TGG + chr3.4:40854698-40854717 MS.gene22186:CDS 30.0%
AAGTCTGCACTTTCAATAAT+TGG + chr3.4:40855743-40855762 MS.gene22186:CDS 30.0%
ATCGTCGAAAATGTTATGTT+TGG + chr3.4:40855107-40855126 MS.gene22186:CDS 30.0%
ATTCCACTCACATTATAACA+TGG - chr3.4:40855593-40855612 None:intergenic 30.0%
GAAAATGTTATGTTTGGTTG+TGG + chr3.4:40855113-40855132 MS.gene22186:CDS 30.0%
GTGAATCTTACAAAACCAAA+TGG + chr3.4:40855068-40855087 MS.gene22186:CDS 30.0%
TAAACTTCATCACACTTCAT+TGG - chr3.4:40855833-40855852 None:intergenic 30.0%
TCACCGTACCAATAAAAATA+CGG - chr3.4:40855005-40855024 None:intergenic 30.0%
TCTTGAATAATCTCATAAGC+TGG - chr3.4:40855512-40855531 None:intergenic 30.0%
TGATCAAAACAAGCATAACA+AGG - chr3.4:40854852-40854871 None:intergenic 30.0%
! AATTTCACATCTTTTGTTGG+TGG + chr3.4:40855335-40855354 MS.gene22186:CDS 30.0%
! ATCTGATGATGTTGTTTGTT+TGG + chr3.4:40855703-40855722 MS.gene22186:CDS 30.0%
! ATTATTGAAAGTGCAGACTT+AGG - chr3.4:40855743-40855762 None:intergenic 30.0%
! ATTTTCGACGATTTTGAACA+CGG - chr3.4:40855099-40855118 None:intergenic 30.0%
!! AATTGCTATTTCTATCCACA+AGG - chr3.4:40855253-40855272 None:intergenic 30.0%
!! ATCAGAGAAAAATGTTTTGG+TGG - chr3.4:40854789-40854808 None:intergenic 30.0%
!! GTTGTTTGTTTGGCTATTTT+AGG + chr3.4:40855713-40855732 MS.gene22186:CDS 30.0%
!! TGTCCGTATTTTTATTGGTA+CGG + chr3.4:40854999-40855018 MS.gene22186:CDS 30.0%
AAGCCATGTTATAATGTGAG+TGG + chr3.4:40855587-40855606 MS.gene22186:CDS 35.0%
AATCCAGTTAAGATCACTAC+CGG - chr3.4:40854820-40854839 None:intergenic 35.0%
AGCTGTGAAGCAAATGATAA+TGG - chr3.4:40855197-40855216 None:intergenic 35.0%
ATTTCTATCCACAAGGCAAT+AGG - chr3.4:40855246-40855265 None:intergenic 35.0%
GAAATGGAATTGCCTGAATT+TGG + chr3.4:40855617-40855636 MS.gene22186:CDS 35.0%
GATGTGAAGAAATCTAGACT+TGG + chr3.4:40855800-40855819 MS.gene22186:CDS 35.0%
TACATCGATGAAATACTCAC+CGG - chr3.4:40854754-40854773 None:intergenic 35.0%
TAGTTTCCGGTGAAATATTC+CGG - chr3.4:40854684-40854703 None:intergenic 35.0%
TCACAGCTTCAAAATCTCTA+TGG + chr3.4:40855209-40855228 MS.gene22186:CDS 35.0%
TCTTCCTAATCCTAACAAAC+CGG - chr3.4:40855171-40855190 None:intergenic 35.0%
TGTGAGTGGAATTGAAGAAA+TGG + chr3.4:40855601-40855620 MS.gene22186:CDS 35.0%
TTATGTTTGGTTGTGGTCAT+TGG + chr3.4:40855120-40855139 MS.gene22186:CDS 35.0%
! AAGCTGAGTTGAATAGTTTC+CGG - chr3.4:40854697-40854716 None:intergenic 35.0%
! GGTGGTACAATAATTGATTC+TGG + chr3.4:40855461-40855480 MS.gene22186:CDS 35.0%
! TTCATTTTCCTATTGCCTTG+TGG + chr3.4:40855235-40855254 MS.gene22186:CDS 35.0%
! TTTTCTCTGATACTTGACAC+CGG + chr3.4:40854798-40854817 MS.gene22186:CDS 35.0%
!! TTAAATGAAGATGGTTCTGG+TGG + chr3.4:40855443-40855462 MS.gene22186:CDS 35.0%
TGGAATAGAGGACTATTTCG+CGG + chr3.4:40855140-40855159 MS.gene22186:CDS 40.0%
TTGAACACGGATTTCCCATT+TGG - chr3.4:40855086-40855105 None:intergenic 40.0%
! AAAACTGACTCCTGATTCCA+AGG - chr3.4:40854727-40854746 None:intergenic 40.0%
! GTTTTCAGATGGAGCTGTTT+GGG + chr3.4:40855646-40855665 MS.gene22186:CDS 40.0%
! TGTTTTCAGATGGAGCTGTT+TGG + chr3.4:40855645-40855664 MS.gene22186:CDS 40.0%
! TTGGAATCAGGAGTCAGTTT+TGG + chr3.4:40854726-40854745 MS.gene22186:CDS 40.0%
!! GGTGATAGTTCTAACACAAC+TGG + chr3.4:40855020-40855039 MS.gene22186:CDS 40.0%
!! GGTTTGTTAGGATTAGGAAG+AGG + chr3.4:40855170-40855189 MS.gene22186:CDS 40.0%
AAAGAAGTCCTATAAGCCGC+CGG + chr3.4:40854638-40854657 MS.gene22186:CDS 45.0%
ACACCGGTAGTGATCTTAAC+TGG + chr3.4:40854814-40854833 MS.gene22186:CDS 45.0%
GCATCACCGGAATATTTCAC+CGG + chr3.4:40854675-40854694 MS.gene22186:CDS 45.0%
GGTTGTGGTCATTGGAATAG+AGG + chr3.4:40855128-40855147 MS.gene22186:CDS 45.0%
TGATTCTCCTCAGCCTTACA+AGG - chr3.4:40854975-40854994 None:intergenic 45.0%
!! GCTTCCGGTTTGTTAGGATT+AGG + chr3.4:40855164-40855183 MS.gene22186:CDS 45.0%
!!! TCAGGAGTCAGTTTTGGTTC+CGG + chr3.4:40854732-40854751 MS.gene22186:CDS 45.0%
AACTCAGCTTATGGCCACCT+TGG + chr3.4:40854707-40854726 MS.gene22186:CDS 50.0%
CTTATGGCCACCTTGGAATC+AGG + chr3.4:40854714-40854733 MS.gene22186:CDS 50.0%
TTACAAGGCTGAGGAGGATC+AGG - chr3.4:40854960-40854979 None:intergenic 50.0%
! ACTGACTCCTGATTCCAAGG+TGG - chr3.4:40854724-40854743 None:intergenic 50.0%
! TGATCCTCCTCAGCCTTGTA+AGG + chr3.4:40854959-40854978 MS.gene22186:CDS 50.0%
TCAGCCTTACAAGGCTGAGG+AGG - chr3.4:40854966-40854985 None:intergenic 55.0%
TCCTCAGCCTTACAAGGCTG+AGG - chr3.4:40854969-40854988 None:intergenic 55.0%
TGTTGCTCTCGCAGCATCAC+CGG + chr3.4:40854662-40854681 MS.gene22186:CDS 55.0%
! TCCTCAGCCTTGTAAGGCTG+AGG + chr3.4:40854965-40854984 MS.gene22186:CDS 55.0%
AGCAACAGCCGGCGGCTTAT+AGG - chr3.4:40854649-40854668 None:intergenic 60.0%
GATGCTGCGAGAGCAACAGC+CGG - chr3.4:40854660-40854679 None:intergenic 60.0%
GGACTATTTCGCGGCGCTTC+CGG + chr3.4:40855149-40855168 MS.gene22186:CDS 60.0%
! CGCGGCGCTTCCGGTTTGTT+AGG + chr3.4:40855158-40855177 MS.gene22186:CDS 65.0%
GCTGCGAGAGCAACAGCCGG+CGG - chr3.4:40854657-40854676 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 40854615 40855853 40854615 ID=MS.gene22186
chr3.4 mRNA 40854615 40855853 40854615 ID=MS.gene22186.t1;Parent=MS.gene22186
chr3.4 exon 40854615 40855853 40854615 ID=MS.gene22186.t1.exon1;Parent=MS.gene22186.t1
chr3.4 CDS 40854615 40855853 40854615 ID=cds.MS.gene22186.t1;Parent=MS.gene22186.t1
Gene Sequence

>MS.gene22186

ATGCAAAAAACAAAGGAACAATCAAAGAAGTCCTATAAGCCGCCGGCTGTTGCTCTCGCAGCATCACCGGAATATTTCACCGGAAACTATTCAACTCAGCTTATGGCCACCTTGGAATCAGGAGTCAGTTTTGGTTCCGGTGAGTATTTCATCGATGTATTCATCGGTACGCCACCAAAACATTTTTCTCTGATACTTGACACCGGTAGTGATCTTAACTGGATTCAATGTGTTCCTTGTTATGCTTGTTTTGATCAAAATGGACCATATTTTGATCCTAAAGATTCCACTTCTTTTAAGAACATAAGTTGTCACGATTCGCGATGTCAACTCGTGTCATCGCCTGATCCTCCTCAGCCTTGTAAGGCTGAGGAGAATCAAACTTGTCCGTATTTTTATTGGTACGGTGATAGTTCTAACACAACTGGCGATTTCGCACTTGAAACATTTCGAGTGAATCTTACAAAACCAAATGGGAAATCCGTGTTCAAAATCGTCGAAAATGTTATGTTTGGTTGTGGTCATTGGAATAGAGGACTATTTCGCGGCGCTTCCGGTTTGTTAGGATTAGGAAGAGGACCATTATCATTTGCTTCACAGCTTCAAAATCTCTATGGTCATTCATTTTCCTATTGCCTTGTGGATAGAAATAGCAATTCAAGTGTTAGTAGCAAATTGTTATTTGGTGAAAACAAAAAGCTTCTTAGTAACCCTAATTTGAATTTCACATCTTTTGTTGGTGGAAAAGAGAACACATTTTACTATGTTGAAATAAAATCTATTATAGTTGGTGGTGAAGTGCTAAAAATACCAGAAGAAACTTGGAAATTAAATGAAGATGGTTCTGGTGGTACAATAATTGATTCTGGTACAACTTTAACTTATTTCGCTCGACCAGCTTATGAGATTATTCAAGAAGCATTCATGAAGAAAATTAAGGGTTATGCAATTGTTGAAAGTTTTGCACCATTAAAGCCATGTTATAATGTGAGTGGAATTGAAGAAATGGAATTGCCTGAATTTGGAATATTGTTTTCAGATGGAGCTGTTTGGGATTTTCCTGTTGAGAATTATTTTATTCAGATTGAATCTGATGATGTTGTTTGTTTGGCTATTTTAGGGACTCCTAAGTCTGCACTTTCAATAATTGGAAATTATCAGCAACAGAATTTTCATATTTTGTATGATGTGAAGAAATCTAGACTTGGTTATGCTCCAATGAAGTGTGATGAAGTTTAA
Protein sequence

>MS.gene22186.t1

MQKTKEQSKKSYKPPAVALAASPEYFTGNYSTQLMATLESGVSFGSGEYFIDVFIGTPPKHFSLILDTGSDLNWIQCVPCYACFDQNGPYFDPKDSTSFKNISCHDSRCQLVSSPDPPQPCKAEENQTCPYFYWYGDSSNTTGDFALETFRVNLTKPNGKSVFKIVENVMFGCGHWNRGLFRGASGLLGLGRGPLSFASQLQNLYGHSFSYCLVDRNSNSSVSSKLLFGENKKLLSNPNLNFTSFVGGKENTFYYVEIKSIIVGGEVLKIPEETWKLNEDGSGGTIIDSGTTLTYFARPAYEIIQEAFMKKIKGYAIVESFAPLKPCYNVSGIEEMELPEFGILFSDGAVWDFPVENYFIQIESDDVVCLAILGTPKSALSIIGNYQQQNFHILYDVKKSRLGYAPMKCDEV