AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22514

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22514.t1 MTR_7g118170 97.948 536 9 1 1 536 1 534 0.0 1082
MS.gene22514.t1 MTR_1g076430 64.741 502 166 5 30 531 17 507 0.0 671
MS.gene22514.t1 MTR_1g041085 64.940 502 165 5 30 531 23 513 0.0 661
MS.gene22514.t1 MTR_2g054940 65.070 501 164 4 31 531 17 506 0.0 660
MS.gene22514.t1 MTR_4g113280 62.675 501 165 4 31 531 6 484 0.0 635
MS.gene22514.t1 MTR_4g070270 60.594 505 185 5 27 530 11 502 0.0 628
MS.gene22514.t1 MTR_7g081695 62.600 500 158 7 31 527 17 490 0.0 616
MS.gene22514.t1 MTR_3g105550 55.664 512 212 7 27 530 32 536 0.0 597
MS.gene22514.t1 MTR_1g110930 57.800 500 197 6 33 531 5 491 0.0 590
MS.gene22514.t1 MTR_5g056510 57.058 503 196 6 33 531 5 491 0.0 575
MS.gene22514.t1 MTR_7g118160 61.605 461 129 4 70 528 3 417 0.0 566
MS.gene22514.t1 MTR_4g054630 54.390 467 205 4 67 532 9 468 0.0 521
MS.gene22514.t1 MTR_4g054415 57.882 425 173 3 109 532 8 427 0.0 519
MS.gene22514.t1 MTR_6g074880 56.481 432 177 4 100 530 47 468 4.01e-179 513
MS.gene22514.t1 MTR_6g032965 58.056 391 156 3 146 533 2 387 6.02e-165 473
MS.gene22514.t1 MTR_2g013740 49.091 495 229 5 27 519 27 500 1.08e-164 477
MS.gene22514.t1 MTR_2g007020 51.923 416 192 4 118 532 45 453 7.70e-155 450
MS.gene22514.t1 MTR_2g436480 48.605 430 209 5 105 530 30 451 6.91e-147 430
MS.gene22514.t1 MTR_1g102290 46.229 411 217 2 118 528 78 484 4.76e-133 395
MS.gene22514.t1 MTR_3g072980 45.476 420 218 5 114 530 74 485 5.05e-127 380
MS.gene22514.t1 MTR_3g072940 45.476 420 218 5 114 530 74 485 5.94e-127 380
MS.gene22514.t1 MTR_4g062270 43.256 430 225 7 106 527 15 433 1.83e-122 368
MS.gene22514.t1 MTR_2g096210 43.237 451 233 8 105 536 4 450 3.30e-122 367
MS.gene22514.t1 MTR_4g062170 42.661 436 227 6 106 527 15 441 4.53e-119 359
MS.gene22514.t1 MTR_4g062190 41.972 436 231 5 106 527 15 442 2.60e-118 357
MS.gene22514.t1 MTR_6g051440 45.631 412 213 5 121 529 80 483 2.73e-118 358
MS.gene22514.t1 MTR_1g115560 40.455 440 238 5 107 527 17 451 2.73e-117 355
MS.gene22514.t1 MTR_4g054260 53.992 263 98 4 271 532 87 327 6.65e-93 287
MS.gene22514.t1 MTR_4g053905 58.434 166 64 2 367 532 1 161 1.04e-63 205
MS.gene22514.t1 MTR_4g054265 40.708 113 67 0 107 219 6 118 1.26e-24 99.4
MS.gene22514.t1 MTR_4g078730 26.102 295 178 12 206 495 120 379 5.07e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22514.t1 AT1G01120 72.968 529 134 5 4 530 5 526 0.0 797
MS.gene22514.t1 AT2G26640 64.683 504 161 5 31 531 15 504 0.0 670
MS.gene22514.t1 AT1G04220 63.905 507 167 7 31 531 20 516 0.0 659
MS.gene22514.t1 AT1G19440 60.396 505 186 5 27 530 23 514 0.0 625
MS.gene22514.t1 AT5G43760 63.179 497 170 5 39 531 34 521 0.0 623
MS.gene22514.t1 AT2G16280 60.158 507 178 9 30 530 22 510 0.0 613
MS.gene22514.t1 AT2G26250 54.753 526 204 7 27 528 33 548 0.0 587
MS.gene22514.t1 AT1G68530 56.000 500 206 5 33 531 6 492 0.0 578
MS.gene22514.t1 AT4G34510 57.948 497 187 8 37 530 8 485 0.0 575
MS.gene22514.t1 AT2G46720 62.238 429 159 1 102 530 40 465 0.0 574
MS.gene22514.t1 AT1G25450 55.912 499 208 5 33 531 1 487 0.0 568
MS.gene22514.t1 AT3G10280 61.072 429 157 2 102 530 40 458 0.0 556
MS.gene22514.t1 AT2G15090 51.452 482 220 3 59 532 6 481 0.0 518
MS.gene22514.t1 AT4G34250 56.561 442 175 5 104 534 56 491 3.43e-180 516
MS.gene22514.t1 AT4G34520 56.561 442 172 5 104 530 54 490 1.79e-178 512
MS.gene22514.t1 AT1G71160 50.116 431 206 3 106 535 31 453 7.02e-156 453
MS.gene22514.t1 AT5G49070 46.187 459 235 6 82 533 5 458 5.93e-146 428
MS.gene22514.t1 AT3G52160 45.521 413 189 3 108 520 74 450 5.18e-131 389
MS.gene22514.t1 AT3G52160 47.709 371 158 3 150 520 3 337 4.23e-122 362
MS.gene22514.t1 AT1G68530 46.982 381 186 5 33 408 6 375 8.70e-121 360
MS.gene22514.t1 AT2G28630 41.725 429 238 4 110 527 19 446 4.25e-120 362
MS.gene22514.t1 AT2G28630 41.725 429 238 4 110 527 19 446 4.25e-120 362
MS.gene22514.t1 AT5G04530 41.355 428 237 6 107 527 17 437 1.39e-116 353
MS.gene22514.t1 AT1G07720 42.991 428 223 6 116 527 25 447 4.46e-116 352
MS.gene22514.t1 AT1G07720 42.991 428 223 6 116 527 68 490 6.41e-116 353
MS.gene22514.t1 AT5G49070 41.748 309 174 3 82 384 5 313 2.65e-81 256

Find 135 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGTCTCGTCTTCTTTC+TGG 0.110532 7.3:+515448 None:intergenic
TCTTGTCATCAGCTCCTTTA+TGG 0.143442 7.3:+515487 None:intergenic
TGTATGAACCAGCTTGTATT+TGG 0.221689 7.3:+515518 None:intergenic
AAGACCACCGCGTAGGAATT+TGG 0.226830 7.3:+515669 None:intergenic
TTCATTGGCTAGAGAATTAA+TGG 0.254813 7.3:-515415 MS.gene22514:CDS
AACATGAACTGTTCAGAAAA+TGG 0.259600 7.3:+515336 None:intergenic
GGTTAGATCAGAGGACTTAT+TGG 0.265749 7.3:+515545 None:intergenic
CAAAATCCGACGACGTTTAC+CGG 0.274993 7.3:-516414 MS.gene22514:CDS
CTTGTCATCAGCTCCTTTAT+GGG 0.289862 7.3:+515488 None:intergenic
AATTTGAAATCTGGTATATA+AGG 0.292144 7.3:+515258 None:intergenic
ATTTGCTTTCAAAAGATCTT+TGG 0.295644 7.3:+515692 None:intergenic
GCAAAATCCACCAGGTAAAT+TGG 0.298744 7.3:+516134 None:intergenic
GGGATTGGCGGGGCAACCCC+TGG 0.302657 7.3:-514960 MS.gene22514:CDS
GAAGCAGAAGCTGTAATGTT+TGG 0.314657 7.3:-515912 MS.gene22514:CDS
TCAAAAGCTAATTTGAAATC+TGG 0.331655 7.3:+515249 None:intergenic
CGGGTCTGGCAGATTGCATT+CGG 0.336102 7.3:-515042 MS.gene22514:CDS
TTGCAAAAGGGGATCGGGTC+TGG 0.336451 7.3:-515056 MS.gene22514:CDS
TGCGCGACTTGCCCGTCGTC+GGG 0.341272 7.3:-514981 MS.gene22514:CDS
GCCCGTCGTCGGGGATTGGC+GGG 0.352548 7.3:-514971 MS.gene22514:CDS
TTAACCCTCAATTGGTACTT+TGG 0.358565 7.3:-515633 MS.gene22514:CDS
GGACTTGACATTATTCCATT+TGG 0.369284 7.3:+515966 None:intergenic
CGGATGACTCTGCATAGATT+TGG 0.389216 7.3:-515138 MS.gene22514:CDS
GCTTCTTTCATGCATAGATT+TGG 0.389782 7.3:+515942 None:intergenic
GCAAAGGGCCGGGTTGCAAA+AGG 0.390952 7.3:-515069 MS.gene22514:CDS
AGAGAATTAATGGCTGTTGC+AGG 0.392253 7.3:-515405 MS.gene22514:CDS
GTTAAAATGTTAGAAGATAG+TGG 0.394444 7.3:-516065 MS.gene22514:CDS
AGGGAAAGTTGGTGTTTCAT+TGG 0.396299 7.3:-515430 MS.gene22514:CDS
ACACTTCAAGCAGTTCTCTA+TGG 0.396346 7.3:-515113 MS.gene22514:CDS
ACTTGCCCGTCGTCGGGGAT+TGG 0.409147 7.3:-514975 MS.gene22514:CDS
AAGCTATAATCTCGGAGGTA+TGG 0.411775 7.3:-515748 MS.gene22514:CDS
AAAACAAACATAACAACGTT+GGG 0.411988 7.3:-515372 MS.gene22514:CDS
TTAGAAGATAGTGGCGAATT+CGG 0.414252 7.3:-516056 MS.gene22514:CDS
AAACTCAAGTACGTAAAGTT+AGG 0.423051 7.3:-516374 MS.gene22514:CDS
CATAACAACGTTGGGTCCAT+TGG 0.424394 7.3:-515364 MS.gene22514:CDS
CCAACTCAGTGAGTGGCATA+TGG 0.428746 7.3:-515169 MS.gene22514:CDS
TGGCAGATTGCATTCGGGTC+GGG 0.430711 7.3:-515036 MS.gene22514:CDS
GGAGGTATGGGTTGTAGTGC+TGG 0.432856 7.3:-515735 MS.gene22514:CDS
CGGAGAATTTAACCCTCAAT+TGG 0.433036 7.3:-515641 MS.gene22514:CDS
ATTGTTCTACTCTTCCTCTT+CGG 0.433972 7.3:-516182 MS.gene22514:CDS
TTGCTGCTGAAGAAAGGTTC+AGG 0.437231 7.3:-515288 MS.gene22514:CDS
CCGGGTTGCAAAAGGGGATC+GGG 0.444082 7.3:-515061 MS.gene22514:CDS
CTGGCAGATTGCATTCGGGT+CGG 0.444799 7.3:-515037 MS.gene22514:CDS
GCCGGGTTGCAAAAGGGGAT+CGG 0.445235 7.3:-515062 MS.gene22514:CDS
CAGTTCTCTATGGTATGAAC+TGG 0.451448 7.3:-515103 MS.gene22514:CDS
CATACAGTTAGAACCCATAA+AGG 0.451862 7.3:-515501 MS.gene22514:CDS
CAAAGGGCCGGGTTGCAAAA+GGG 0.466708 7.3:-515068 MS.gene22514:CDS
ACAAGAATGTCAATGTCTCT+TGG 0.469200 7.3:+515852 None:intergenic
GTATAGCGGCGCCACCCATT+CGG 0.470845 7.3:+515574 None:intergenic
TCTTTCATGCATAGATTTGG+TGG 0.474801 7.3:+515945 None:intergenic
TTGCAGAAAGTGAAGGAGTT+GGG 0.476301 7.3:+515808 None:intergenic
GTGCGCGACTTGCCCGTCGT+CGG 0.478086 7.3:-514982 MS.gene22514:CDS
CATTTCCAAAGTACCAATTG+AGG 0.478571 7.3:+515628 None:intergenic
GGATTGGCGGGGCAACCCCT+GGG 0.483271 7.3:-514959 MS.gene22514:CDS
TGCCCGTCGTCGGGGATTGG+CGG 0.485832 7.3:-514972 MS.gene22514:CDS
AGCTATAATCTCGGAGGTAT+GGG 0.488082 7.3:-515747 MS.gene22514:CDS
GAACCAGCTTGTATTTGGAC+CGG 0.488419 7.3:+515523 None:intergenic
AAGTACGTAAAGTTAGGTTA+TGG 0.495016 7.3:-516368 MS.gene22514:CDS
GAACATTTCTGTATTCATGC+TGG 0.495485 7.3:-515228 MS.gene22514:CDS
CCCCGCCAATCCCCGACGAC+GGG 0.497979 7.3:+514970 None:intergenic
TTCACCGTCTGTAGAAAATC+CGG 0.498288 7.3:+516395 None:intergenic
AGGAAGTTGCTGCTGAAGAA+AGG 0.501320 7.3:-515294 MS.gene22514:CDS
AAAGGGCCGGGTTGCAAAAG+GGG 0.505317 7.3:-515067 MS.gene22514:CDS
ATTTCCAAAGTACCAATTGA+GGG 0.506557 7.3:+515629 None:intergenic
GGGTCTGGCAGATTGCATTC+GGG 0.514216 7.3:-515041 MS.gene22514:CDS
AATTGAACCGATGGCTGTAT+CGG 0.514947 7.3:+516202 None:intergenic
GACGAAACTTCCTTGCCAAA+TGG 0.515853 7.3:-515981 MS.gene22514:CDS
TTATACTGAGGCAAAGGGCC+GGG 0.516750 7.3:-515079 MS.gene22514:CDS
GAAAACAAACATAACAACGT+TGG 0.519196 7.3:-515373 MS.gene22514:CDS
TGTTTGTTCTTAGCTTGTAG+TGG 0.521220 7.3:+515775 None:intergenic
ACAGAAACAGGGACGCTGAC+GGG 0.521485 7.3:+514916 None:intergenic
AACATTAAAAGCTATAATCT+CGG 0.522267 7.3:-515756 MS.gene22514:CDS
CTTATACTGAGGCAAAGGGC+CGG 0.522593 7.3:-515080 MS.gene22514:CDS
CCCGTCGTCGGGGATTGGCG+GGG 0.523629 7.3:-514970 MS.gene22514:CDS
TACCAGAAAGAAGACGAGAC+AGG 0.524700 7.3:-515450 MS.gene22514:CDS
AATCCACCAGGTAAATTGGT+GGG 0.541242 7.3:+516138 None:intergenic
ATTGCAGAAAGTGAAGGAGT+TGG 0.552174 7.3:+515807 None:intergenic
GAAGACGAGACAGGGAAAGT+TGG 0.555142 7.3:-515441 MS.gene22514:CDS
TCAGAAAATGGCAAAACCAA+TGG 0.557047 7.3:+515348 None:intergenic
GCCCCGCCAATCCCCGACGA+CGG 0.559697 7.3:+514969 None:intergenic
ACGATCCCCACCAATTTACC+TGG 0.560276 7.3:-516144 MS.gene22514:CDS
CCATATGCCACTCACTGAGT+TGG 0.569030 7.3:+515169 None:intergenic
GCAAGAGTTGTGAGAGACGG+TGG 0.571620 7.3:+516249 None:intergenic
TGGCTGTATCGGTGTCAAGT+TGG 0.573119 7.3:+516213 None:intergenic
GACGACGGGCAAGTCGCGCA+CGG 0.573305 7.3:+514984 None:intergenic
ACTGGCTTATACTGAGGCAA+AGG 0.574125 7.3:-515085 MS.gene22514:CDS
CTTATCAACAGAGTCATCCC+AGG 0.582184 7.3:+514942 None:intergenic
ATCCACCAGGTAAATTGGTG+GGG 0.585932 7.3:+516139 None:intergenic
AACCCGGTCCAAATACAAGC+TGG 0.586438 7.3:-515526 MS.gene22514:CDS
TGACATTATTCCATTTGGCA+AGG 0.590067 7.3:+515971 None:intergenic
CTGGCTTATACTGAGGCAAA+GGG 0.590480 7.3:-515084 MS.gene22514:CDS
CATTTCTGTATTCATGCTGG+TGG 0.590993 7.3:-515225 MS.gene22514:CDS
AACAGAAACAGGGACGCTGA+CGG 0.594758 7.3:+514915 None:intergenic
AACCAGCTTGTATTTGGACC+GGG 0.596597 7.3:+515524 None:intergenic
GAGTAGAACAATTGAACCGA+TGG 0.597177 7.3:+516193 None:intergenic
AAATCCACCAGGTAAATTGG+TGG 0.601519 7.3:+516137 None:intergenic
AACCTGCTCTTGACAAAATG+CGG 0.602486 7.3:+516009 None:intergenic
GTATGAACTGGCTTATACTG+AGG 0.604352 7.3:-515091 MS.gene22514:CDS
TGTGAAAATGAGAATGGTTG+TGG 0.605218 7.3:+516331 None:intergenic
ATCCCCACCAATTTACCTGG+TGG 0.607799 7.3:-516141 MS.gene22514:CDS
ATCGGTGAGTTGGAACTGAA+CGG 0.608509 7.3:+516280 None:intergenic
ACAATCATTGCAGAAAGTGA+AGG 0.609013 7.3:+515801 None:intergenic
ACCAGAAAGAAGACGAGACA+GGG 0.610150 7.3:-515449 MS.gene22514:CDS
GTGTAACAGTGCTGTGTGGA+AGG 0.610162 7.3:-515007 MS.gene22514:CDS
TTTGGACCGGGTTAGATCAG+AGG 0.612315 7.3:+515536 None:intergenic
TTTAAGAAGCAACAGAAACA+GGG 0.614888 7.3:+514905 None:intergenic
CGGTGAGGAAGAGTAAGAGA+GGG 0.614974 7.3:+516300 None:intergenic
GCGCGACTTGCCCGTCGTCG+GGG 0.623964 7.3:-514980 MS.gene22514:CDS
ATAAGTCCTCTGATCTAACC+CGG 0.625331 7.3:-515542 MS.gene22514:CDS
CGAGCAAGAGTTGTGAGAGA+CGG 0.627180 7.3:+516246 None:intergenic
GACGAGTGTGAAAATGAGAA+TGG 0.628164 7.3:+516325 None:intergenic
ACGGTGAGGAAGAGTAAGAG+AGG 0.633167 7.3:+516299 None:intergenic
CTACGCGGTGGTCTTGAGCA+CGG 0.635511 7.3:-515661 MS.gene22514:CDS
GAAAATCCGGTAAACGTCGT+CGG 0.635960 7.3:+516408 None:intergenic
GCTACAAACCGGAGAAAGAG+CGG 0.638965 7.3:-516109 MS.gene22514:CDS
AGTGGCATATGGAACCGTCT+CGG 0.641136 7.3:-515158 MS.gene22514:CDS
TGTAGCACGCAAAATCCACC+AGG 0.643428 7.3:+516126 None:intergenic
CTTGACACCGATACAGCCAT+CGG 0.644102 7.3:-516209 MS.gene22514:CDS
GGGTCGCAACAGCATAGACA+TGG 0.648812 7.3:-516492 MS.gene22514:CDS
TTATCAACAGAGTCATCCCA+GGG 0.649243 7.3:+514943 None:intergenic
AGAATCTCCAACTCAGTGAG+TGG 0.655119 7.3:-515176 MS.gene22514:CDS
TGTATCGGTGTCAAGTTGGA+CGG 0.658526 7.3:+516217 None:intergenic
CTATGCAGAGTCATCCGAGA+CGG 0.659543 7.3:+515144 None:intergenic
CTTATTGGAAAGAAGTATAG+CGG 0.661996 7.3:+515560 None:intergenic
CATGGATAAAGAAAGACTAA+CGG 0.662335 7.3:-516474 MS.gene22514:CDS
AAATCCAAATTCCTACGCGG+TGG 0.667104 7.3:-515673 MS.gene22514:CDS
GAAAGAGCGGAAAATAGCAG+TGG 0.668978 7.3:-516096 MS.gene22514:CDS
CGTGCTCAAGACCACCGCGT+AGG 0.672026 7.3:+515662 None:intergenic
ATTAAAAGCTATAATCTCGG+AGG 0.675727 7.3:-515753 MS.gene22514:CDS
TCGCGTAGTACAGACCGAAG+AGG 0.689821 7.3:+516168 None:intergenic
TTTCAAAGATTCAACCTCCG+CGG 0.705846 7.3:-516441 MS.gene22514:CDS
TGAGTTGGAACTGAACGGTG+AGG 0.720930 7.3:+516285 None:intergenic
TTAAGTGTAACAGTGCTGTG+TGG 0.723760 7.3:-515011 MS.gene22514:CDS
GGATAAAGAAAGACTAACGG+CGG 0.724763 7.3:-516471 MS.gene22514:CDS
AGCAAATCCAAATTCCTACG+CGG 0.758527 7.3:-515676 MS.gene22514:CDS
TATCAACAGAGTCATCCCAG+GGG 0.818112 7.3:+514944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATTAAAAGCTATAATCT+CGG - chr7.3:515644-515663 MS.gene22514:CDS 20.0%
!!! AATTTGAAATCTGGTATATA+AGG + chr7.3:516145-516164 None:intergenic 20.0%
! AAAACAAACATAACAACGTT+GGG - chr7.3:516028-516047 MS.gene22514:CDS 25.0%
! ATTTGCTTTCAAAAGATCTT+TGG + chr7.3:515711-515730 None:intergenic 25.0%
! GTTAAAATGTTAGAAGATAG+TGG - chr7.3:515335-515354 MS.gene22514:CDS 25.0%
!! TCAAAAGCTAATTTGAAATC+TGG + chr7.3:516154-516173 None:intergenic 25.0%
!! TTTTTGTGTCTTTGGTTAAA+AGG - chr7.3:516086-516105 MS.gene22514:CDS 25.0%
AAACTCAAGTACGTAAAGTT+AGG - chr7.3:515026-515045 MS.gene22514:CDS 30.0%
AACATGAACTGTTCAGAAAA+TGG + chr7.3:516067-516086 None:intergenic 30.0%
AAGTACGTAAAGTTAGGTTA+TGG - chr7.3:515032-515051 MS.gene22514:CDS 30.0%
ATTAAAAGCTATAATCTCGG+AGG - chr7.3:515647-515666 MS.gene22514:CDS 30.0%
CATGGATAAAGAAAGACTAA+CGG - chr7.3:514926-514945 MS.gene22514:CDS 30.0%
GAAAACAAACATAACAACGT+TGG - chr7.3:516027-516046 MS.gene22514:CDS 30.0%
! ATTTCCAAAGTACCAATTGA+GGG + chr7.3:515774-515793 None:intergenic 30.0%
! CTTATTGGAAAGAAGTATAG+CGG + chr7.3:515843-515862 None:intergenic 30.0%
! TCTAATTGCATTTTCCGAAT+GGG - chr7.3:515812-515831 MS.gene22514:CDS 30.0%
! TTCATTGGCTAGAGAATTAA+TGG - chr7.3:515985-516004 MS.gene22514:CDS 30.0%
! TTCTAATTGCATTTTCCGAA+TGG - chr7.3:515811-515830 MS.gene22514:CDS 30.0%
!!! GTTCATGTTTTTTGTGTCTT+TGG - chr7.3:516078-516097 MS.gene22514:CDS 30.0%
ACAAGAATGTCAATGTCTCT+TGG + chr7.3:515551-515570 None:intergenic 35.0%
ACAATCATTGCAGAAAGTGA+AGG + chr7.3:515602-515621 None:intergenic 35.0%
ATTGTTCTACTCTTCCTCTT+CGG - chr7.3:515218-515237 MS.gene22514:CDS 35.0%
CATACAGTTAGAACCCATAA+AGG - chr7.3:515899-515918 MS.gene22514:CDS 35.0%
GAACATTTCTGTATTCATGC+TGG - chr7.3:516172-516191 MS.gene22514:CDS 35.0%
GCTTCTTTCATGCATAGATT+TGG + chr7.3:515461-515480 None:intergenic 35.0%
GGACTTGACATTATTCCATT+TGG + chr7.3:515437-515456 None:intergenic 35.0%
TCAGAAAATGGCAAAACCAA+TGG + chr7.3:516055-516074 None:intergenic 35.0%
TCTTTCATGCATAGATTTGG+TGG + chr7.3:515458-515477 None:intergenic 35.0%
TGACATTATTCCATTTGGCA+AGG + chr7.3:515432-515451 None:intergenic 35.0%
TGTATGAACCAGCTTGTATT+TGG + chr7.3:515885-515904 None:intergenic 35.0%
TGTGAAAATGAGAATGGTTG+TGG + chr7.3:515072-515091 None:intergenic 35.0%
TTAACCCTCAATTGGTACTT+TGG - chr7.3:515767-515786 MS.gene22514:CDS 35.0%
! ATTTTGTCAAGAGCAGGTTT+AGG - chr7.3:515395-515414 MS.gene22514:CDS 35.0%
! CATTTCCAAAGTACCAATTG+AGG + chr7.3:515775-515794 None:intergenic 35.0%
! TTAGAAGATAGTGGCGAATT+CGG - chr7.3:515344-515363 MS.gene22514:CDS 35.0%
! TTTACCGGATTTTCTACAGA+CGG - chr7.3:515001-515020 MS.gene22514:CDS 35.0%
!! TGTTTGTTCTTAGCTTGTAG+TGG + chr7.3:515628-515647 None:intergenic 35.0%
AAATCCACCAGGTAAATTGG+TGG + chr7.3:515266-515285 None:intergenic 40.0%
AACCTGCTCTTGACAAAATG+CGG + chr7.3:515394-515413 None:intergenic 40.0%
AAGCTATAATCTCGGAGGTA+TGG - chr7.3:515652-515671 MS.gene22514:CDS 40.0%
AATCCACCAGGTAAATTGGT+GGG + chr7.3:515265-515284 None:intergenic 40.0%
AATTGAACCGATGGCTGTAT+CGG + chr7.3:515201-515220 None:intergenic 40.0%
ACACTTCAAGCAGTTCTCTA+TGG - chr7.3:516287-516306 MS.gene22514:CDS 40.0%
AGAGAATTAATGGCTGTTGC+AGG - chr7.3:515995-516014 MS.gene22514:CDS 40.0%
AGCAAATCCAAATTCCTACG+CGG - chr7.3:515724-515743 MS.gene22514:CDS 40.0%
ATAAGTCCTCTGATCTAACC+CGG - chr7.3:515858-515877 MS.gene22514:CDS 40.0%
ATTGCAGAAAGTGAAGGAGT+TGG + chr7.3:515596-515615 None:intergenic 40.0%
CAGTTCTCTATGGTATGAAC+TGG - chr7.3:516297-516316 MS.gene22514:CDS 40.0%
CATTTCTGTATTCATGCTGG+TGG - chr7.3:516175-516194 MS.gene22514:CDS 40.0%
CGGAGAATTTAACCCTCAAT+TGG - chr7.3:515759-515778 MS.gene22514:CDS 40.0%
CTTGTCATCAGCTCCTTTAT+GGG + chr7.3:515915-515934 None:intergenic 40.0%
GAAGCAGAAGCTGTAATGTT+TGG - chr7.3:515488-515507 MS.gene22514:CDS 40.0%
GACGAGTGTGAAAATGAGAA+TGG + chr7.3:515078-515097 None:intergenic 40.0%
GAGTAGAACAATTGAACCGA+TGG + chr7.3:515210-515229 None:intergenic 40.0%
GCAAAATCCACCAGGTAAAT+TGG + chr7.3:515269-515288 None:intergenic 40.0%
GGATAAAGAAAGACTAACGG+CGG - chr7.3:514929-514948 MS.gene22514:CDS 40.0%
GGTTAGATCAGAGGACTTAT+TGG + chr7.3:515858-515877 None:intergenic 40.0%
TCTTGTCATCAGCTCCTTTA+TGG + chr7.3:515916-515935 None:intergenic 40.0%
TTAAGTGTAACAGTGCTGTG+TGG - chr7.3:516389-516408 MS.gene22514:CDS 40.0%
TTATCAACAGAGTCATCCCA+GGG + chr7.3:516460-516479 None:intergenic 40.0%
TTCACCGTCTGTAGAAAATC+CGG + chr7.3:515008-515027 None:intergenic 40.0%
TTGCAGAAAGTGAAGGAGTT+GGG + chr7.3:515595-515614 None:intergenic 40.0%
TTTCAAAGATTCAACCTCCG+CGG - chr7.3:514959-514978 MS.gene22514:CDS 40.0%
! AATTGCATTTTCCGAATGGG+TGG - chr7.3:515815-515834 MS.gene22514:CDS 40.0%
! AGCTATAATCTCGGAGGTAT+GGG - chr7.3:515653-515672 MS.gene22514:CDS 40.0%
! GTATGAACTGGCTTATACTG+AGG - chr7.3:516309-516328 MS.gene22514:CDS 40.0%
!! AGGGAAAGTTGGTGTTTCAT+TGG - chr7.3:515970-515989 MS.gene22514:CDS 40.0%
!! GACGCACTTTTTGCTAAAAC+AGG - chr7.3:515518-515537 MS.gene22514:CDS 40.0%
AAATCCAAATTCCTACGCGG+TGG - chr7.3:515727-515746 MS.gene22514:CDS 45.0%
AACCAGCTTGTATTTGGACC+GGG + chr7.3:515879-515898 None:intergenic 45.0%
ACCAGAAAGAAGACGAGACA+GGG - chr7.3:515951-515970 MS.gene22514:CDS 45.0%
AGAATCTCCAACTCAGTGAG+TGG - chr7.3:516224-516243 MS.gene22514:CDS 45.0%
AGGAAGTTGCTGCTGAAGAA+AGG - chr7.3:516106-516125 MS.gene22514:CDS 45.0%
ATCCACCAGGTAAATTGGTG+GGG + chr7.3:515264-515283 None:intergenic 45.0%
CAAAATCCGACGACGTTTAC+CGG - chr7.3:514986-515005 MS.gene22514:CDS 45.0%
CATAACAACGTTGGGTCCAT+TGG - chr7.3:516036-516055 MS.gene22514:CDS 45.0%
CGGATGACTCTGCATAGATT+TGG - chr7.3:516262-516281 MS.gene22514:CDS 45.0%
CTGGCTTATACTGAGGCAAA+GGG - chr7.3:516316-516335 MS.gene22514:CDS 45.0%
CTTATCAACAGAGTCATCCC+AGG + chr7.3:516461-516480 None:intergenic 45.0%
GAAAATCCGGTAAACGTCGT+CGG + chr7.3:514995-515014 None:intergenic 45.0%
GAAAGAGCGGAAAATAGCAG+TGG - chr7.3:515304-515323 MS.gene22514:CDS 45.0%
GAACCAGCTTGTATTTGGAC+CGG + chr7.3:515880-515899 None:intergenic 45.0%
GACGAAACTTCCTTGCCAAA+TGG - chr7.3:515419-515438 MS.gene22514:CDS 45.0%
TACCAGAAAGAAGACGAGAC+AGG - chr7.3:515950-515969 MS.gene22514:CDS 45.0%
TATCAACAGAGTCATCCCAG+GGG + chr7.3:516459-516478 None:intergenic 45.0%
TTGCTGCTGAAGAAAGGTTC+AGG - chr7.3:516112-516131 MS.gene22514:CDS 45.0%
! ACTGGCTTATACTGAGGCAA+AGG - chr7.3:516315-516334 MS.gene22514:CDS 45.0%
! ATCGGTGAGTTGGAACTGAA+CGG + chr7.3:515123-515142 None:intergenic 45.0%
! GGATTTTGCGTGCTACAAAC+CGG - chr7.3:515280-515299 MS.gene22514:CDS 45.0%
!! GCTATTTTCCGCTCTTTCTC+CGG + chr7.3:515302-515321 None:intergenic 45.0%
!! TCGTCGGATTTTGATAACCG+CGG + chr7.3:514979-514998 None:intergenic 45.0%
!! TGTATCGGTGTCAAGTTGGA+CGG + chr7.3:515186-515205 None:intergenic 45.0%
!!! GAGACGGTGGAGTTTTAGAT+CGG + chr7.3:515141-515160 None:intergenic 45.0%
!!! GGAGTTTTAGATCGGTGAGT+TGG + chr7.3:515133-515152 None:intergenic 45.0%
AACAGAAACAGGGACGCTGA+CGG + chr7.3:516488-516507 None:intergenic 50.0%
AACCCGGTCCAAATACAAGC+TGG - chr7.3:515874-515893 MS.gene22514:CDS 50.0%
AAGACCACCGCGTAGGAATT+TGG + chr7.3:515734-515753 None:intergenic 50.0%
ACGATCCCCACCAATTTACC+TGG - chr7.3:515256-515275 MS.gene22514:CDS 50.0%
ACGGTGAGGAAGAGTAAGAG+AGG + chr7.3:515104-515123 None:intergenic 50.0%
AGTGGCATATGGAACCGTCT+CGG - chr7.3:516242-516261 MS.gene22514:CDS 50.0%
ATCCCCACCAATTTACCTGG+TGG - chr7.3:515259-515278 MS.gene22514:CDS 50.0%
CCAACTCAGTGAGTGGCATA+TGG - chr7.3:516231-516250 MS.gene22514:CDS 50.0%
CCATATGCCACTCACTGAGT+TGG + chr7.3:516234-516253 None:intergenic 50.0%
CGAGCAAGAGTTGTGAGAGA+CGG + chr7.3:515157-515176 None:intergenic 50.0%
CGGTGAGGAAGAGTAAGAGA+GGG + chr7.3:515103-515122 None:intergenic 50.0%
CTATGCAGAGTCATCCGAGA+CGG + chr7.3:516259-516278 None:intergenic 50.0%
CTTATACTGAGGCAAAGGGC+CGG - chr7.3:516320-516339 MS.gene22514:CDS 50.0%
CTTGACACCGATACAGCCAT+CGG - chr7.3:515191-515210 MS.gene22514:CDS 50.0%
GAAGACGAGACAGGGAAAGT+TGG - chr7.3:515959-515978 MS.gene22514:CDS 50.0%
GCTACAAACCGGAGAAAGAG+CGG - chr7.3:515291-515310 MS.gene22514:CDS 50.0%
GTGTAACAGTGCTGTGTGGA+AGG - chr7.3:516393-516412 MS.gene22514:CDS 50.0%
TCCCTGTCTCGTCTTCTTTC+TGG + chr7.3:515955-515974 None:intergenic 50.0%
TGTAGCACGCAAAATCCACC+AGG + chr7.3:515277-515296 None:intergenic 50.0%
TTATACTGAGGCAAAGGGCC+GGG - chr7.3:516321-516340 MS.gene22514:CDS 50.0%
! TGAGTTGGAACTGAACGGTG+AGG + chr7.3:515118-515137 None:intergenic 50.0%
! TTTGGACCGGGTTAGATCAG+AGG + chr7.3:515867-515886 None:intergenic 50.0%
!! TCGGATTTTGATAACCGCGG+AGG + chr7.3:514976-514995 None:intergenic 50.0%
!! TGGCTGTATCGGTGTCAAGT+TGG + chr7.3:515190-515209 None:intergenic 50.0%
AAAGGGCCGGGTTGCAAAAG+GGG - chr7.3:516333-516352 MS.gene22514:CDS 55.0%
ACAGAAACAGGGACGCTGAC+GGG + chr7.3:516487-516506 None:intergenic 55.0%
CAAAGGGCCGGGTTGCAAAA+GGG - chr7.3:516332-516351 MS.gene22514:CDS 55.0%
CGGGTCTGGCAGATTGCATT+CGG - chr7.3:516358-516377 MS.gene22514:CDS 55.0%
CTGGCAGATTGCATTCGGGT+CGG - chr7.3:516363-516382 MS.gene22514:CDS 55.0%
GCAAGAGTTGTGAGAGACGG+TGG + chr7.3:515154-515173 None:intergenic 55.0%
GGGTCGCAACAGCATAGACA+TGG - chr7.3:514908-514927 MS.gene22514:CDS 55.0%
GGGTCTGGCAGATTGCATTC+GGG - chr7.3:516359-516378 MS.gene22514:CDS 55.0%
TCGCGTAGTACAGACCGAAG+AGG + chr7.3:515235-515254 None:intergenic 55.0%
TGGCAGATTGCATTCGGGTC+GGG - chr7.3:516364-516383 MS.gene22514:CDS 55.0%
TTGCAAAAGGGGATCGGGTC+TGG - chr7.3:516344-516363 MS.gene22514:CDS 55.0%
! CGCCGCATTTTGTCAAGAGC+AGG - chr7.3:515389-515408 MS.gene22514:CDS 55.0%
! GGAGGTATGGGTTGTAGTGC+TGG - chr7.3:515665-515684 MS.gene22514:CDS 55.0%
CCGGGTTGCAAAAGGGGATC+GGG - chr7.3:516339-516358 MS.gene22514:CDS 60.0%
GCAAAGGGCCGGGTTGCAAA+AGG - chr7.3:516331-516350 MS.gene22514:CDS 60.0%
GCCGGGTTGCAAAAGGGGAT+CGG - chr7.3:516338-516357 MS.gene22514:CDS 60.0%
GTATAGCGGCGCCACCCATT+CGG + chr7.3:515829-515848 None:intergenic 60.0%
! CCCGATCCCCTTTTGCAACC+CGG + chr7.3:516342-516361 None:intergenic 60.0%
! CTACGCGGTGGTCTTGAGCA+CGG - chr7.3:515739-515758 MS.gene22514:CDS 60.0%
ACTTGCCCGTCGTCGGGGAT+TGG - chr7.3:516425-516444 MS.gene22514:CDS 65.0%
CGTGCTCAAGACCACCGCGT+AGG + chr7.3:515741-515760 None:intergenic 65.0%
GACGACGGGCAAGTCGCGCA+CGG + chr7.3:516419-516438 None:intergenic 70.0%
GTGCGCGACTTGCCCGTCGT+CGG - chr7.3:516418-516437 MS.gene22514:CDS 70.0%
TGCCCGTCGTCGGGGATTGG+CGG - chr7.3:516428-516447 MS.gene22514:CDS 70.0%
TGCGCGACTTGCCCGTCGTC+GGG - chr7.3:516419-516438 MS.gene22514:CDS 70.0%
! GGATTGGCGGGGCAACCCCT+GGG - chr7.3:516441-516460 MS.gene22514:CDS 70.0%
CCCCGCCAATCCCCGACGAC+GGG + chr7.3:516433-516452 None:intergenic 75.0%
GCCCCGCCAATCCCCGACGA+CGG + chr7.3:516434-516453 None:intergenic 75.0%
GCGCGACTTGCCCGTCGTCG+GGG - chr7.3:516420-516439 MS.gene22514:CDS 75.0%
! CCCGTCGTCGGGGATTGGCG+GGG - chr7.3:516430-516449 MS.gene22514:CDS 75.0%
! GCCCGTCGTCGGGGATTGGC+GGG - chr7.3:516429-516448 MS.gene22514:CDS 75.0%
! GGGATTGGCGGGGCAACCCC+TGG - chr7.3:516440-516459 MS.gene22514:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 514906 516516 514906 ID=MS.gene22514
chr7.3 mRNA 514906 516516 514906 ID=MS.gene22514.t1;Parent=MS.gene22514
chr7.3 exon 514906 516516 514906 ID=MS.gene22514.t1.exon1;Parent=MS.gene22514.t1
chr7.3 CDS 514906 516516 514906 ID=cds.MS.gene22514.t1;Parent=MS.gene22514.t1
Gene Sequence

>MS.gene22514

ATGGGTCGCAACAGCATAGACATGGATAAAGAAAGACTAACGGCGGAGATGAATTTCAAAGATTCAACCTCCGCGGTTATCAAAATCCGACGACGTTTACCGGATTTTCTACAGACGGTGAAACTCAAGTACGTAAAGTTAGGTTATGGCTATTCATGCAACGCCACAACCATTCTCATTTTCACACTCGTCGTCCCTCTCTTACTCTTCCTCACCGTTCAGTTCCAACTCACCGATCTAAAACTCCACCGTCTCTCACAACTCTTGCTCGAACACACCGTCCAACTTGACACCGATACAGCCATCGGTTCAATTGTTCTACTCTTCCTCTTCGGTCTGTACTACGCGAAACGATCCCCACCAATTTACCTGGTGGATTTTGCGTGCTACAAACCGGAGAAAGAGCGGAAAATAGCAGTGGAATCTTTTGTTAAAATGTTAGAAGATAGTGGCGAATTCGGAGAAGAAACACTTCAATTTCAACGCCGCATTTTGTCAAGAGCAGGTTTAGGCGACGAAACTTCCTTGCCAAATGGAATAATGTCAAGTCCACCAAATCTATGCATGAAAGAAGCGCGTTTAGAAGCAGAAGCTGTAATGTTTGGATCATTAGACGCACTTTTTGCTAAAACAGGCGTTAATCCAAGAGACATTGACATTCTTGTTGTAAATTGTAGTTTGTTTAACCCAACTCCTTCACTTTCTGCAATGATTGTGAACCACTACAAGCTAAGAACAAACATTAAAAGCTATAATCTCGGAGGTATGGGTTGTAGTGCTGGTCTTATTTCAATTGATCTTGCCAAAGATCTTTTGAAAGCAAATCCAAATTCCTACGCGGTGGTCTTGAGCACGGAGAATTTAACCCTCAATTGGTACTTTGGAAATGATCGTTCAATGCTTCTTTCTAATTGCATTTTCCGAATGGGTGGCGCCGCTATACTTCTTTCCAATAAGTCCTCTGATCTAACCCGGTCCAAATACAAGCTGGTTCATACAGTTAGAACCCATAAAGGAGCTGATGACAAGAACTATAACTGTGTTTACCAGAAAGAAGACGAGACAGGGAAAGTTGGTGTTTCATTGGCTAGAGAATTAATGGCTGTTGCAGGAGATGCTTTGAAAACAAACATAACAACGTTGGGTCCATTGGTTTTGCCATTTTCTGAACAGTTCATGTTTTTTGTGTCTTTGGTTAAAAGGAAGTTGCTGCTGAAGAAAGGTTCAGGTGTGAAACCTTATATACCAGATTTCAAATTAGCTTTTGAACATTTCTGTATTCATGCTGGTGGAAGAGCTGTTCTTGATGAGTTGCAGAAGAATCTCCAACTCAGTGAGTGGCATATGGAACCGTCTCGGATGACTCTGCATAGATTTGGAAACACTTCAAGCAGTTCTCTATGGTATGAACTGGCTTATACTGAGGCAAAGGGCCGGGTTGCAAAAGGGGATCGGGTCTGGCAGATTGCATTCGGGTCGGGCTTTAAGTGTAACAGTGCTGTGTGGAAGGCCGTGCGCGACTTGCCCGTCGTCGGGGATTGGCGGGGCAACCCCTGGGATGACTCTGTTGATAAGTATCCCGTCAGCGTCCCTGTTTCTGTTGCTTCTTAA
Protein sequence

>MS.gene22514.t1

MGRNSIDMDKERLTAEMNFKDSTSAVIKIRRRLPDFLQTVKLKYVKLGYGYSCNATTILIFTLVVPLLLFLTVQFQLTDLKLHRLSQLLLEHTVQLDTDTAIGSIVLLFLFGLYYAKRSPPIYLVDFACYKPEKERKIAVESFVKMLEDSGEFGEETLQFQRRILSRAGLGDETSLPNGIMSSPPNLCMKEARLEAEAVMFGSLDALFAKTGVNPRDIDILVVNCSLFNPTPSLSAMIVNHYKLRTNIKSYNLGGMGCSAGLISIDLAKDLLKANPNSYAVVLSTENLTLNWYFGNDRSMLLSNCIFRMGGAAILLSNKSSDLTRSKYKLVHTVRTHKGADDKNYNCVYQKEDETGKVGVSLARELMAVAGDALKTNITTLGPLVLPFSEQFMFFVSLVKRKLLLKKGSGVKPYIPDFKLAFEHFCIHAGGRAVLDELQKNLQLSEWHMEPSRMTLHRFGNTSSSSLWYELAYTEAKGRVAKGDRVWQIAFGSGFKCNSAVWKAVRDLPVVGDWRGNPWDDSVDKYPVSVPVSVAS