AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22515

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22515.t1 MTR_7g118170 97.059 68 2 0 405 472 463 530 2.39e-39 149
MS.gene22515.t1 MTR_7g118160 79.688 64 13 0 405 468 354 417 4.83e-31 124
MS.gene22515.t1 MTR_4g113280 68.657 67 19 1 405 471 420 484 2.88e-23 102
MS.gene22515.t1 MTR_2g054940 68.657 67 19 1 405 471 442 506 4.72e-23 102
MS.gene22515.t1 MTR_1g041085 67.164 67 20 1 405 471 449 513 1.32e-22 100
MS.gene22515.t1 MTR_1g076430 67.164 67 20 1 405 471 443 507 2.41e-22 100
MS.gene22515.t1 MTR_5g056510 65.217 69 17 2 405 471 428 491 6.05e-21 95.9
MS.gene22515.t1 MTR_4g054630 62.121 66 23 1 405 470 403 466 9.13e-21 95.1
MS.gene22515.t1 MTR_1g110930 65.672 67 20 1 405 471 428 491 9.73e-21 95.1
MS.gene22515.t1 MTR_4g054415 62.121 66 23 1 405 470 362 425 1.07e-20 94.7
MS.gene22515.t1 MTR_4g070270 62.121 66 22 1 405 470 440 502 2.98e-20 94.0
MS.gene22515.t1 MTR_7g081695 66.667 63 19 1 405 467 430 490 8.76e-20 93.2
MS.gene22515.t1 MTR_2g007020 61.111 72 23 2 405 475 389 456 1.74e-19 91.3
MS.gene22515.t1 MTR_6g074880 62.121 66 23 1 405 470 405 468 3.49e-19 90.5
MS.gene22515.t1 MTR_4g053905 57.576 66 26 1 405 470 96 159 4.74e-19 84.7
MS.gene22515.t1 MTR_3g105550 62.121 66 23 1 405 470 473 536 2.51e-18 88.2
MS.gene22515.t1 MTR_2g436480 60.606 66 23 1 405 470 389 451 2.49e-17 84.7
MS.gene22515.t1 MTR_6g032965 54.412 68 29 1 405 472 321 386 3.04e-17 84.0
MS.gene22515.t1 MTR_2g096210 60.317 63 24 1 405 467 383 444 7.31e-17 83.2
MS.gene22515.t1 MTR_4g062270 57.143 63 22 2 405 467 376 433 2.95e-15 78.2
MS.gene22515.t1 MTR_4g062170 57.143 63 22 2 405 467 384 441 2.98e-15 78.2
MS.gene22515.t1 MTR_4g062190 56.452 62 22 2 406 467 386 442 6.44e-15 77.4
MS.gene22515.t1 MTR_1g102290 54.688 64 27 1 405 468 423 484 1.82e-14 75.9
MS.gene22515.t1 MTR_3g072980 52.308 65 29 1 405 469 422 484 4.66e-13 71.6
MS.gene22515.t1 MTR_3g072940 52.308 65 29 1 405 469 422 484 4.66e-13 71.6
MS.gene22515.t1 MTR_6g051440 50.769 65 30 1 405 469 421 483 5.26e-13 71.2
MS.gene22515.t1 MTR_2g013740 58.730 63 18 2 402 459 441 500 9.13e-12 67.4
MS.gene22515.t1 MTR_1g115560 46.032 63 29 1 405 467 394 451 1.71e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22515.t1 AT5G43760 68.657 67 20 1 405 471 456 521 1.66e-24 107
MS.gene22515.t1 AT3G10280 71.212 66 19 0 405 470 393 458 6.52e-24 104
MS.gene22515.t1 AT2G46720 71.212 66 19 0 405 470 400 465 7.70e-24 104
MS.gene22515.t1 AT1G01120 71.642 67 16 2 405 470 462 526 3.27e-23 103
MS.gene22515.t1 AT2G26640 67.164 67 20 1 405 471 440 504 1.89e-22 100
MS.gene22515.t1 AT1G04220 62.319 69 24 1 405 471 448 516 1.10e-21 98.6
MS.gene22515.t1 AT2G15090 61.765 68 23 1 405 472 417 481 2.07e-21 97.4
MS.gene22515.t1 AT1G19440 63.636 66 21 1 405 470 452 514 1.12e-20 95.1
MS.gene22515.t1 AT4G34250 63.636 66 21 1 405 470 425 487 1.43e-20 94.7
MS.gene22515.t1 AT1G68530 64.179 67 21 1 405 471 429 492 2.57e-20 94.0
MS.gene22515.t1 AT4G34510 65.672 67 18 2 405 470 423 485 3.22e-20 93.6
MS.gene22515.t1 AT2G16280 62.687 67 20 2 405 470 448 510 3.29e-19 90.9
MS.gene22515.t1 AT2G26250 65.625 64 20 1 405 468 487 548 1.04e-18 89.4
MS.gene22515.t1 AT4G34520 57.576 66 25 1 405 470 428 490 2.07e-18 88.2
MS.gene22515.t1 AT1G25450 59.701 67 24 1 405 471 424 487 3.03e-18 87.8
MS.gene22515.t1 AT5G49070 61.194 67 23 1 405 471 393 456 1.57e-17 85.5
MS.gene22515.t1 AT1G71160 61.538 65 20 2 405 468 386 446 1.79e-17 85.1
MS.gene22515.t1 AT2G28630 60.317 63 24 1 405 467 385 446 1.55e-16 82.4
MS.gene22515.t1 AT2G28630 60.317 63 24 1 405 467 385 446 1.55e-16 82.4
MS.gene22515.t1 AT1G07720 58.730 63 25 1 405 467 429 490 4.19e-16 81.3
MS.gene22515.t1 AT1G07720 58.730 63 25 1 405 467 386 447 6.36e-16 80.5
MS.gene22515.t1 AT3G52160 56.250 64 21 2 402 460 276 337 1.02e-14 75.9
MS.gene22515.t1 AT3G52160 56.250 64 21 2 402 460 389 450 1.34e-14 76.3
MS.gene22515.t1 AT5G04530 44.262 61 29 1 407 467 382 437 2.19e-11 66.2

Find 126 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGTCTCGTCTTCTTTC+TGG 0.110532 7.3:-541567 None:intergenic
TCTTGTCATCAGCTCCTTTA+TGG 0.143442 7.3:-541528 None:intergenic
TGTATGAACCAGCTTGTATT+TGG 0.221689 7.3:-541497 None:intergenic
AAGACCACCGCGTAGGAATT+TGG 0.226830 7.3:-541346 None:intergenic
TTCATTGGCTAGAGAATTAA+TGG 0.254813 7.3:+541600 MS.gene22515:CDS
AACATGAACTGTTCAGAAAA+TGG 0.259600 7.3:-541679 None:intergenic
GGTTAGATCAGAGGACTTAT+TGG 0.265749 7.3:-541470 None:intergenic
CAAAATCCGACGACGTTTAC+CGG 0.274993 7.3:+540601 MS.gene22515:CDS
CTTGTCATCAGCTCCTTTAT+GGG 0.289862 7.3:-541527 None:intergenic
ATTTGCTTTCAAAAGATCTT+TGG 0.295644 7.3:-541323 None:intergenic
GCAAAATCCACCAGGTAAAT+TGG 0.298744 7.3:-540881 None:intergenic
GGGATTGGCGGGGCAACCCC+TGG 0.302657 7.3:+542055 MS.gene22515:CDS
GAAGCAGAAGCTGTAATGTT+TGG 0.314657 7.3:+541103 MS.gene22515:CDS
CGGGTCTGGCAGATTGCATT+CGG 0.336102 7.3:+541973 MS.gene22515:CDS
TTGCAAAAGGGGATCGGGTC+TGG 0.336451 7.3:+541959 MS.gene22515:CDS
TGCGCGACTTGCCCGTCGTC+GGG 0.341272 7.3:+542034 MS.gene22515:CDS
GCCCGTCGTCGGGGATTGGC+GGG 0.352548 7.3:+542044 MS.gene22515:CDS
TTAACCCTCAATTGGTACTT+TGG 0.358565 7.3:+541382 MS.gene22515:CDS
GGACTTGACATTATTCCATT+TGG 0.369284 7.3:-541049 None:intergenic
GCTTCTTTCATGCATAGATT+TGG 0.389782 7.3:-541073 None:intergenic
GCAAAGGGCCGGGTTGCAAA+AGG 0.390952 7.3:+541946 MS.gene22515:CDS
AGAGAATTAATGGCTGTTGC+AGG 0.392253 7.3:+541610 MS.gene22515:CDS
GTTAAAATGTTAGAAGATAG+TGG 0.394444 7.3:+540950 MS.gene22515:CDS
AGGGAAAGTTGGTGTTTCAT+TGG 0.396299 7.3:+541585 MS.gene22515:CDS
ACACTTCAAGCAGTTCTCTA+TGG 0.396346 7.3:+541902 MS.gene22515:intron
ACTTGCCCGTCGTCGGGGAT+TGG 0.409147 7.3:+542040 MS.gene22515:CDS
AAGCTATAATCTCGGAGGTA+TGG 0.411775 7.3:+541267 MS.gene22515:CDS
AAAACAAACATAACAACGTT+GGG 0.411988 7.3:+541643 MS.gene22515:CDS
TTAGAAGATAGTGGCGAATT+CGG 0.414252 7.3:+540959 MS.gene22515:CDS
AAACTCAAGTACGTAAAGTT+AGG 0.423051 7.3:+540641 MS.gene22515:CDS
CATAACAACGTTGGGTCCAT+TGG 0.424394 7.3:+541651 MS.gene22515:CDS
TGGCAGATTGCATTCGGGTC+GGG 0.430711 7.3:+541979 MS.gene22515:CDS
GGAGGTATGGGTTGTAGTGC+TGG 0.432856 7.3:+541280 MS.gene22515:CDS
CGGAGAATTTAACCCTCAAT+TGG 0.433036 7.3:+541374 MS.gene22515:CDS
ATTGTTCTACTCTTCCTCTT+CGG 0.433972 7.3:+540833 MS.gene22515:CDS
TTGCTGCTGAAGAAAGGTTC+AGG 0.437231 7.3:+541727 MS.gene22515:CDS
CCGGGTTGCAAAAGGGGATC+GGG 0.444082 7.3:+541954 MS.gene22515:CDS
CTGGCAGATTGCATTCGGGT+CGG 0.444799 7.3:+541978 MS.gene22515:CDS
GCCGGGTTGCAAAAGGGGAT+CGG 0.445235 7.3:+541953 MS.gene22515:CDS
CAGTTCTCTATGGTATGAAC+TGG 0.451448 7.3:+541912 MS.gene22515:intron
CATACAGTTAGAACCCATAA+AGG 0.451862 7.3:+541514 MS.gene22515:CDS
CAGAAACAGGGGACGCTGAC+GGG 0.461766 7.3:-542099 None:intergenic
CAAAGGGCCGGGTTGCAAAA+GGG 0.466708 7.3:+541947 MS.gene22515:CDS
ACAAGAATGTCAATGTCTCT+TGG 0.469200 7.3:-541163 None:intergenic
GTATAGCGGCGCCACCCATT+CGG 0.470845 7.3:-541441 None:intergenic
TCTTTCATGCATAGATTTGG+TGG 0.474801 7.3:-541070 None:intergenic
TTGCAGAAAGTGAAGGAGTT+GGG 0.476301 7.3:-541207 None:intergenic
GTGCGCGACTTGCCCGTCGT+CGG 0.478086 7.3:+542033 MS.gene22515:CDS
CATTTCCAAAGTACCAATTG+AGG 0.478571 7.3:-541387 None:intergenic
GGATTGGCGGGGCAACCCCT+GGG 0.483271 7.3:+542056 MS.gene22515:CDS
TGCCCGTCGTCGGGGATTGG+CGG 0.485832 7.3:+542043 MS.gene22515:CDS
AGCTATAATCTCGGAGGTAT+GGG 0.488082 7.3:+541268 MS.gene22515:CDS
GAACCAGCTTGTATTTGGAC+CGG 0.488419 7.3:-541492 None:intergenic
AAGTACGTAAAGTTAGGTTA+TGG 0.495016 7.3:+540647 MS.gene22515:CDS
CCCCGCCAATCCCCGACGAC+GGG 0.497979 7.3:-542045 None:intergenic
TTCACCGTCTGTAGAAAATC+CGG 0.498288 7.3:-540620 None:intergenic
AGGAAGTTGCTGCTGAAGAA+AGG 0.501320 7.3:+541721 MS.gene22515:CDS
AAAGGGCCGGGTTGCAAAAG+GGG 0.505317 7.3:+541948 MS.gene22515:CDS
ATTTCCAAAGTACCAATTGA+GGG 0.506557 7.3:-541386 None:intergenic
GGGTCTGGCAGATTGCATTC+GGG 0.514216 7.3:+541974 MS.gene22515:CDS
AATTGAACCGATGGCTGTAT+CGG 0.514947 7.3:-540813 None:intergenic
GACGAAACTTCCTTGCCAAA+TGG 0.515853 7.3:+541034 MS.gene22515:CDS
TTATACTGAGGCAAAGGGCC+GGG 0.516750 7.3:+541936 MS.gene22515:CDS
GAAAACAAACATAACAACGT+TGG 0.519196 7.3:+541642 MS.gene22515:CDS
TGTTTGTTCTTAGCTTGTAG+TGG 0.521220 7.3:-541240 None:intergenic
AACATTAAAAGCTATAATCT+CGG 0.522267 7.3:+541259 MS.gene22515:CDS
CTTATACTGAGGCAAAGGGC+CGG 0.522593 7.3:+541935 MS.gene22515:CDS
CCCGTCGTCGGGGATTGGCG+GGG 0.523629 7.3:+542045 MS.gene22515:CDS
TACCAGAAAGAAGACGAGAC+AGG 0.524700 7.3:+541565 MS.gene22515:CDS
AATCCACCAGGTAAATTGGT+GGG 0.541242 7.3:-540877 None:intergenic
ATTGCAGAAAGTGAAGGAGT+TGG 0.552174 7.3:-541208 None:intergenic
GAAGACGAGACAGGGAAAGT+TGG 0.555142 7.3:+541574 MS.gene22515:CDS
TCAGAAAATGGCAAAACCAA+TGG 0.557047 7.3:-541667 None:intergenic
GCCCCGCCAATCCCCGACGA+CGG 0.559697 7.3:-542046 None:intergenic
ACGATCCCCACCAATTTACC+TGG 0.560276 7.3:+540871 MS.gene22515:CDS
GCAAGAGTTGTGAGAGACGG+TGG 0.571620 7.3:-540766 None:intergenic
TGGCTGTATCGGTGTCAAGT+TGG 0.573119 7.3:-540802 None:intergenic
GACGACGGGCAAGTCGCGCA+CGG 0.573305 7.3:-542031 None:intergenic
ACTGGCTTATACTGAGGCAA+AGG 0.574125 7.3:+541930 MS.gene22515:CDS
CTTATCAACAGAGTCATCCC+AGG 0.582184 7.3:-542073 None:intergenic
TTTAAGAAGCAACAGAAACA+GGG 0.582343 7.3:-542111 None:intergenic
ATCCACCAGGTAAATTGGTG+GGG 0.585932 7.3:-540876 None:intergenic
AACCCGGTCCAAATACAAGC+TGG 0.586438 7.3:+541489 MS.gene22515:CDS
TGACATTATTCCATTTGGCA+AGG 0.590067 7.3:-541044 None:intergenic
CTGGCTTATACTGAGGCAAA+GGG 0.590480 7.3:+541931 MS.gene22515:CDS
ACAGAAACAGGGGACGCTGA+CGG 0.593288 7.3:-542100 None:intergenic
AACCAGCTTGTATTTGGACC+GGG 0.596597 7.3:-541491 None:intergenic
GAGTAGAACAATTGAACCGA+TGG 0.597177 7.3:-540822 None:intergenic
AAATCCACCAGGTAAATTGG+TGG 0.601519 7.3:-540878 None:intergenic
AACCTGCTCTTGACAAAATG+CGG 0.602486 7.3:-541006 None:intergenic
GTATGAACTGGCTTATACTG+AGG 0.604352 7.3:+541924 MS.gene22515:CDS
TGTGAAAATGAGAATGGTTG+TGG 0.605218 7.3:-540684 None:intergenic
ATCCCCACCAATTTACCTGG+TGG 0.607799 7.3:+540874 MS.gene22515:CDS
ATCGGTGAGTTGGAACTGAA+CGG 0.608509 7.3:-540735 None:intergenic
ACAATCATTGCAGAAAGTGA+AGG 0.609013 7.3:-541214 None:intergenic
ACCAGAAAGAAGACGAGACA+GGG 0.610150 7.3:+541566 MS.gene22515:CDS
GTGTAACAGTGCTGTGTGGA+AGG 0.610162 7.3:+542008 MS.gene22515:CDS
TTTGGACCGGGTTAGATCAG+AGG 0.612315 7.3:-541479 None:intergenic
CGGTGAGGAAGAGTAAGAGA+GGG 0.614974 7.3:-540715 None:intergenic
GCGCGACTTGCCCGTCGTCG+GGG 0.623964 7.3:+542035 MS.gene22515:CDS
ATAAGTCCTCTGATCTAACC+CGG 0.625331 7.3:+541473 MS.gene22515:CDS
CGAGCAAGAGTTGTGAGAGA+CGG 0.627180 7.3:-540769 None:intergenic
GACGAGTGTGAAAATGAGAA+TGG 0.628164 7.3:-540690 None:intergenic
ACGGTGAGGAAGAGTAAGAG+AGG 0.633167 7.3:-540716 None:intergenic
CTACGCGGTGGTCTTGAGCA+CGG 0.635511 7.3:+541354 MS.gene22515:CDS
GAAAATCCGGTAAACGTCGT+CGG 0.635960 7.3:-540607 None:intergenic
GCTACAAACCGGAGAAAGAG+CGG 0.638965 7.3:+540906 MS.gene22515:CDS
TGTAGCACGCAAAATCCACC+AGG 0.643428 7.3:-540889 None:intergenic
CTTGACACCGATACAGCCAT+CGG 0.644102 7.3:+540806 MS.gene22515:CDS
GGGTCGCAACAGCATAGACA+TGG 0.648812 7.3:+540523 None:intergenic
TTATCAACAGAGTCATCCCA+GGG 0.649243 7.3:-542072 None:intergenic
TGTATCGGTGTCAAGTTGGA+CGG 0.658526 7.3:-540798 None:intergenic
CTTATTGGAAAGAAGTATAG+CGG 0.661996 7.3:-541455 None:intergenic
CATGGATAAAGAAAGACTAA+CGG 0.662335 7.3:+540541 MS.gene22515:CDS
AAATCCAAATTCCTACGCGG+TGG 0.667104 7.3:+541342 MS.gene22515:CDS
GAAAGAGCGGAAAATAGCAG+TGG 0.668978 7.3:+540919 MS.gene22515:CDS
CGTGCTCAAGACCACCGCGT+AGG 0.672026 7.3:-541353 None:intergenic
TTAAGAAGCAACAGAAACAG+GGG 0.674943 7.3:-542110 None:intergenic
ATTAAAAGCTATAATCTCGG+AGG 0.675727 7.3:+541262 MS.gene22515:CDS
TCGCGTAGTACAGACCGAAG+AGG 0.689821 7.3:-540847 None:intergenic
TTTCAAAGATTCAACCTCCG+CGG 0.705846 7.3:+540574 MS.gene22515:CDS
TGAGTTGGAACTGAACGGTG+AGG 0.720930 7.3:-540730 None:intergenic
TTAAGTGTAACAGTGCTGTG+TGG 0.723760 7.3:+542004 MS.gene22515:CDS
GGATAAAGAAAGACTAACGG+CGG 0.724763 7.3:+540544 MS.gene22515:CDS
AGCAAATCCAAATTCCTACG+CGG 0.758527 7.3:+541339 MS.gene22515:CDS
TATCAACAGAGTCATCCCAG+GGG 0.818112 7.3:-542071 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATTAAAAGCTATAATCT+CGG + chr7.3:541259-541278 MS.gene22515:CDS 20.0%
!!! AATTTGAAATCTGGTATATA+AGG - chr7.3:541760-541779 None:intergenic 20.0%
! AAAACAAACATAACAACGTT+GGG + chr7.3:541643-541662 MS.gene22515:CDS 25.0%
! ATTTGCTTTCAAAAGATCTT+TGG - chr7.3:541326-541345 None:intergenic 25.0%
! GTTAAAATGTTAGAAGATAG+TGG + chr7.3:540950-540969 MS.gene22515:CDS 25.0%
!! TCAAAAGCTAATTTGAAATC+TGG - chr7.3:541769-541788 None:intergenic 25.0%
!! TTTTTGTGTCTTTGGTTAAA+AGG + chr7.3:541701-541720 MS.gene22515:CDS 25.0%
AAACTCAAGTACGTAAAGTT+AGG + chr7.3:540641-540660 MS.gene22515:CDS 30.0%
AACATGAACTGTTCAGAAAA+TGG - chr7.3:541682-541701 None:intergenic 30.0%
AAGTACGTAAAGTTAGGTTA+TGG + chr7.3:540647-540666 MS.gene22515:CDS 30.0%
ATTAAAAGCTATAATCTCGG+AGG + chr7.3:541262-541281 MS.gene22515:CDS 30.0%
CATGGATAAAGAAAGACTAA+CGG + chr7.3:540541-540560 MS.gene22515:CDS 30.0%
GAAAACAAACATAACAACGT+TGG + chr7.3:541642-541661 MS.gene22515:CDS 30.0%
! ATTTCCAAAGTACCAATTGA+GGG - chr7.3:541389-541408 None:intergenic 30.0%
! CTTATTGGAAAGAAGTATAG+CGG - chr7.3:541458-541477 None:intergenic 30.0%
! TCTAATTGCATTTTCCGAAT+GGG + chr7.3:541427-541446 MS.gene22515:CDS 30.0%
! TTCATTGGCTAGAGAATTAA+TGG + chr7.3:541600-541619 MS.gene22515:CDS 30.0%
! TTCTAATTGCATTTTCCGAA+TGG + chr7.3:541426-541445 MS.gene22515:CDS 30.0%
!!! GTTCATGTTTTTTGTGTCTT+TGG + chr7.3:541693-541712 MS.gene22515:CDS 30.0%
ACAAGAATGTCAATGTCTCT+TGG - chr7.3:541166-541185 None:intergenic 35.0%
ACAATCATTGCAGAAAGTGA+AGG - chr7.3:541217-541236 None:intergenic 35.0%
ATTGTTCTACTCTTCCTCTT+CGG + chr7.3:540833-540852 MS.gene22515:CDS 35.0%
CATACAGTTAGAACCCATAA+AGG + chr7.3:541514-541533 MS.gene22515:CDS 35.0%
GAACATTTCTGTATTCATGC+TGG + chr7.3:541787-541806 MS.gene22515:intron 35.0%
GCTTCTTTCATGCATAGATT+TGG - chr7.3:541076-541095 None:intergenic 35.0%
GGACTTGACATTATTCCATT+TGG - chr7.3:541052-541071 None:intergenic 35.0%
TCAGAAAATGGCAAAACCAA+TGG - chr7.3:541670-541689 None:intergenic 35.0%
TCTTTCATGCATAGATTTGG+TGG - chr7.3:541073-541092 None:intergenic 35.0%
TGACATTATTCCATTTGGCA+AGG - chr7.3:541047-541066 None:intergenic 35.0%
TGTATGAACCAGCTTGTATT+TGG - chr7.3:541500-541519 None:intergenic 35.0%
TGTGAAAATGAGAATGGTTG+TGG - chr7.3:540687-540706 None:intergenic 35.0%
TTAACCCTCAATTGGTACTT+TGG + chr7.3:541382-541401 MS.gene22515:CDS 35.0%
TTAAGAAGCAACAGAAACAG+GGG - chr7.3:542113-542132 None:intergenic 35.0%
! ATTTTGTCAAGAGCAGGTTT+AGG + chr7.3:541010-541029 MS.gene22515:CDS 35.0%
! CATTTCCAAAGTACCAATTG+AGG - chr7.3:541390-541409 None:intergenic 35.0%
! TTAGAAGATAGTGGCGAATT+CGG + chr7.3:540959-540978 MS.gene22515:CDS 35.0%
! TTTACCGGATTTTCTACAGA+CGG + chr7.3:540616-540635 MS.gene22515:CDS 35.0%
!! TGTTTGTTCTTAGCTTGTAG+TGG - chr7.3:541243-541262 None:intergenic 35.0%
AAATCCACCAGGTAAATTGG+TGG - chr7.3:540881-540900 None:intergenic 40.0%
AACCTGCTCTTGACAAAATG+CGG - chr7.3:541009-541028 None:intergenic 40.0%
AAGCTATAATCTCGGAGGTA+TGG + chr7.3:541267-541286 MS.gene22515:CDS 40.0%
AATCCACCAGGTAAATTGGT+GGG - chr7.3:540880-540899 None:intergenic 40.0%
AATTGAACCGATGGCTGTAT+CGG - chr7.3:540816-540835 None:intergenic 40.0%
ACACTTCAAGCAGTTCTCTA+TGG + chr7.3:541902-541921 MS.gene22515:intron 40.0%
AGAGAATTAATGGCTGTTGC+AGG + chr7.3:541610-541629 MS.gene22515:CDS 40.0%
AGCAAATCCAAATTCCTACG+CGG + chr7.3:541339-541358 MS.gene22515:CDS 40.0%
ATAAGTCCTCTGATCTAACC+CGG + chr7.3:541473-541492 MS.gene22515:CDS 40.0%
ATTGCAGAAAGTGAAGGAGT+TGG - chr7.3:541211-541230 None:intergenic 40.0%
CAGTTCTCTATGGTATGAAC+TGG + chr7.3:541912-541931 MS.gene22515:intron 40.0%
CATTTCTGTATTCATGCTGG+TGG + chr7.3:541790-541809 MS.gene22515:intron 40.0%
CGGAGAATTTAACCCTCAAT+TGG + chr7.3:541374-541393 MS.gene22515:CDS 40.0%
CTTGTCATCAGCTCCTTTAT+GGG - chr7.3:541530-541549 None:intergenic 40.0%
GAAGCAGAAGCTGTAATGTT+TGG + chr7.3:541103-541122 MS.gene22515:CDS 40.0%
GACGAGTGTGAAAATGAGAA+TGG - chr7.3:540693-540712 None:intergenic 40.0%
GAGTAGAACAATTGAACCGA+TGG - chr7.3:540825-540844 None:intergenic 40.0%
GCAAAATCCACCAGGTAAAT+TGG - chr7.3:540884-540903 None:intergenic 40.0%
GGATAAAGAAAGACTAACGG+CGG + chr7.3:540544-540563 MS.gene22515:CDS 40.0%
GGTTAGATCAGAGGACTTAT+TGG - chr7.3:541473-541492 None:intergenic 40.0%
TCTTGTCATCAGCTCCTTTA+TGG - chr7.3:541531-541550 None:intergenic 40.0%
TTAAGTGTAACAGTGCTGTG+TGG + chr7.3:542004-542023 MS.gene22515:CDS 40.0%
TTATCAACAGAGTCATCCCA+GGG - chr7.3:542075-542094 None:intergenic 40.0%
TTCACCGTCTGTAGAAAATC+CGG - chr7.3:540623-540642 None:intergenic 40.0%
TTGCAGAAAGTGAAGGAGTT+GGG - chr7.3:541210-541229 None:intergenic 40.0%
TTTCAAAGATTCAACCTCCG+CGG + chr7.3:540574-540593 MS.gene22515:CDS 40.0%
! AATTGCATTTTCCGAATGGG+TGG + chr7.3:541430-541449 MS.gene22515:CDS 40.0%
! AGCTATAATCTCGGAGGTAT+GGG + chr7.3:541268-541287 MS.gene22515:CDS 40.0%
! GTATGAACTGGCTTATACTG+AGG + chr7.3:541924-541943 MS.gene22515:CDS 40.0%
!! AGGGAAAGTTGGTGTTTCAT+TGG + chr7.3:541585-541604 MS.gene22515:CDS 40.0%
!! GACGCACTTTTTGCTAAAAC+AGG + chr7.3:541133-541152 MS.gene22515:CDS 40.0%
AAATCCAAATTCCTACGCGG+TGG + chr7.3:541342-541361 MS.gene22515:CDS 45.0%
AACCAGCTTGTATTTGGACC+GGG - chr7.3:541494-541513 None:intergenic 45.0%
ACCAGAAAGAAGACGAGACA+GGG + chr7.3:541566-541585 MS.gene22515:CDS 45.0%
AGAATCTCCAACTCAGTGAG+TGG + chr7.3:541839-541858 MS.gene22515:intron 45.0%
AGGAAGTTGCTGCTGAAGAA+AGG + chr7.3:541721-541740 MS.gene22515:CDS 45.0%
ATCCACCAGGTAAATTGGTG+GGG - chr7.3:540879-540898 None:intergenic 45.0%
CAAAATCCGACGACGTTTAC+CGG + chr7.3:540601-540620 MS.gene22515:CDS 45.0%
CATAACAACGTTGGGTCCAT+TGG + chr7.3:541651-541670 MS.gene22515:CDS 45.0%
CGGATGACTCTGCATAGATT+TGG + chr7.3:541877-541896 MS.gene22515:intron 45.0%
CTGGCTTATACTGAGGCAAA+GGG + chr7.3:541931-541950 MS.gene22515:CDS 45.0%
CTTATCAACAGAGTCATCCC+AGG - chr7.3:542076-542095 None:intergenic 45.0%
GAAAATCCGGTAAACGTCGT+CGG - chr7.3:540610-540629 None:intergenic 45.0%
GAAAGAGCGGAAAATAGCAG+TGG + chr7.3:540919-540938 MS.gene22515:CDS 45.0%
GAACCAGCTTGTATTTGGAC+CGG - chr7.3:541495-541514 None:intergenic 45.0%
GACGAAACTTCCTTGCCAAA+TGG + chr7.3:541034-541053 MS.gene22515:CDS 45.0%
TACCAGAAAGAAGACGAGAC+AGG + chr7.3:541565-541584 MS.gene22515:CDS 45.0%
TATCAACAGAGTCATCCCAG+GGG - chr7.3:542074-542093 None:intergenic 45.0%
TTGCTGCTGAAGAAAGGTTC+AGG + chr7.3:541727-541746 MS.gene22515:CDS 45.0%
! ACTGGCTTATACTGAGGCAA+AGG + chr7.3:541930-541949 MS.gene22515:CDS 45.0%
! ATCGGTGAGTTGGAACTGAA+CGG - chr7.3:540738-540757 None:intergenic 45.0%
! GGATTTTGCGTGCTACAAAC+CGG + chr7.3:540895-540914 MS.gene22515:CDS 45.0%
!! GCTATTTTCCGCTCTTTCTC+CGG - chr7.3:540917-540936 None:intergenic 45.0%
!! TCGTCGGATTTTGATAACCG+CGG - chr7.3:540594-540613 None:intergenic 45.0%
!! TGTATCGGTGTCAAGTTGGA+CGG - chr7.3:540801-540820 None:intergenic 45.0%
!!! GAGACGGTGGAGTTTTAGAT+CGG - chr7.3:540756-540775 None:intergenic 45.0%
!!! GGAGTTTTAGATCGGTGAGT+TGG - chr7.3:540748-540767 None:intergenic 45.0%
AACCCGGTCCAAATACAAGC+TGG + chr7.3:541489-541508 MS.gene22515:CDS 50.0%
AAGACCACCGCGTAGGAATT+TGG - chr7.3:541349-541368 None:intergenic 50.0%
ACGATCCCCACCAATTTACC+TGG + chr7.3:540871-540890 MS.gene22515:CDS 50.0%
ACGGTGAGGAAGAGTAAGAG+AGG - chr7.3:540719-540738 None:intergenic 50.0%
AGTGGCATATGGAACCGTCT+CGG + chr7.3:541857-541876 MS.gene22515:intron 50.0%
ATCCCCACCAATTTACCTGG+TGG + chr7.3:540874-540893 MS.gene22515:CDS 50.0%
CCAACTCAGTGAGTGGCATA+TGG + chr7.3:541846-541865 MS.gene22515:intron 50.0%
CCATATGCCACTCACTGAGT+TGG - chr7.3:541849-541868 None:intergenic 50.0%
CGAGCAAGAGTTGTGAGAGA+CGG - chr7.3:540772-540791 None:intergenic 50.0%
CGGTGAGGAAGAGTAAGAGA+GGG - chr7.3:540718-540737 None:intergenic 50.0%
CTATGCAGAGTCATCCGAGA+CGG - chr7.3:541874-541893 None:intergenic 50.0%
CTTATACTGAGGCAAAGGGC+CGG + chr7.3:541935-541954 MS.gene22515:CDS 50.0%
CTTGACACCGATACAGCCAT+CGG + chr7.3:540806-540825 MS.gene22515:CDS 50.0%
GAAGACGAGACAGGGAAAGT+TGG + chr7.3:541574-541593 MS.gene22515:CDS 50.0%
GCTACAAACCGGAGAAAGAG+CGG + chr7.3:540906-540925 MS.gene22515:CDS 50.0%
GTGTAACAGTGCTGTGTGGA+AGG + chr7.3:542008-542027 MS.gene22515:CDS 50.0%
TCCCTGTCTCGTCTTCTTTC+TGG - chr7.3:541570-541589 None:intergenic 50.0%
TGTAGCACGCAAAATCCACC+AGG - chr7.3:540892-540911 None:intergenic 50.0%
TTATACTGAGGCAAAGGGCC+GGG + chr7.3:541936-541955 MS.gene22515:CDS 50.0%
! TGAGTTGGAACTGAACGGTG+AGG - chr7.3:540733-540752 None:intergenic 50.0%
! TTTGGACCGGGTTAGATCAG+AGG - chr7.3:541482-541501 None:intergenic 50.0%
!! TCGGATTTTGATAACCGCGG+AGG - chr7.3:540591-540610 None:intergenic 50.0%
!! TGGCTGTATCGGTGTCAAGT+TGG - chr7.3:540805-540824 None:intergenic 50.0%
AAAGGGCCGGGTTGCAAAAG+GGG + chr7.3:541948-541967 MS.gene22515:CDS 55.0%
ACAGAAACAGGGGACGCTGA+CGG - chr7.3:542103-542122 None:intergenic 55.0%
CAAAGGGCCGGGTTGCAAAA+GGG + chr7.3:541947-541966 MS.gene22515:CDS 55.0%
CGGGTCTGGCAGATTGCATT+CGG + chr7.3:541973-541992 MS.gene22515:CDS 55.0%
CTGGCAGATTGCATTCGGGT+CGG + chr7.3:541978-541997 MS.gene22515:CDS 55.0%
GCAAGAGTTGTGAGAGACGG+TGG - chr7.3:540769-540788 None:intergenic 55.0%
GGGTCTGGCAGATTGCATTC+GGG + chr7.3:541974-541993 MS.gene22515:CDS 55.0%
TCGCGTAGTACAGACCGAAG+AGG - chr7.3:540850-540869 None:intergenic 55.0%
TGGCAGATTGCATTCGGGTC+GGG + chr7.3:541979-541998 MS.gene22515:CDS 55.0%
TTGCAAAAGGGGATCGGGTC+TGG + chr7.3:541959-541978 MS.gene22515:CDS 55.0%
! CGCCGCATTTTGTCAAGAGC+AGG + chr7.3:541004-541023 MS.gene22515:CDS 55.0%
! GGAGGTATGGGTTGTAGTGC+TGG + chr7.3:541280-541299 MS.gene22515:CDS 55.0%
CAGAAACAGGGGACGCTGAC+GGG - chr7.3:542102-542121 None:intergenic 60.0%
CCGGGTTGCAAAAGGGGATC+GGG + chr7.3:541954-541973 MS.gene22515:CDS 60.0%
GCAAAGGGCCGGGTTGCAAA+AGG + chr7.3:541946-541965 MS.gene22515:CDS 60.0%
GCCGGGTTGCAAAAGGGGAT+CGG + chr7.3:541953-541972 MS.gene22515:CDS 60.0%
GTATAGCGGCGCCACCCATT+CGG - chr7.3:541444-541463 None:intergenic 60.0%
! CCCGATCCCCTTTTGCAACC+CGG - chr7.3:541957-541976 None:intergenic 60.0%
! CTACGCGGTGGTCTTGAGCA+CGG + chr7.3:541354-541373 MS.gene22515:CDS 60.0%
ACTTGCCCGTCGTCGGGGAT+TGG + chr7.3:542040-542059 MS.gene22515:CDS 65.0%
CGTGCTCAAGACCACCGCGT+AGG - chr7.3:541356-541375 None:intergenic 65.0%
GACGACGGGCAAGTCGCGCA+CGG - chr7.3:542034-542053 None:intergenic 70.0%
GTGCGCGACTTGCCCGTCGT+CGG + chr7.3:542033-542052 MS.gene22515:CDS 70.0%
TGCCCGTCGTCGGGGATTGG+CGG + chr7.3:542043-542062 MS.gene22515:CDS 70.0%
TGCGCGACTTGCCCGTCGTC+GGG + chr7.3:542034-542053 MS.gene22515:CDS 70.0%
! GGATTGGCGGGGCAACCCCT+GGG + chr7.3:542056-542075 MS.gene22515:CDS 70.0%
CCCCGCCAATCCCCGACGAC+GGG - chr7.3:542048-542067 None:intergenic 75.0%
GCCCCGCCAATCCCCGACGA+CGG - chr7.3:542049-542068 None:intergenic 75.0%
GCGCGACTTGCCCGTCGTCG+GGG + chr7.3:542035-542054 MS.gene22515:CDS 75.0%
! CCCGTCGTCGGGGATTGGCG+GGG + chr7.3:542045-542064 MS.gene22515:CDS 75.0%
! GCCCGTCGTCGGGGATTGGC+GGG + chr7.3:542044-542063 MS.gene22515:CDS 75.0%
! GGGATTGGCGGGGCAACCCC+TGG + chr7.3:542055-542074 MS.gene22515:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 540535 542132 540535 ID=MS.gene22515
chr7.3 mRNA 540535 542132 540535 ID=MS.gene22515.t1;Parent=MS.gene22515
chr7.3 exon 540535 541748 540535 ID=MS.gene22515.t1.exon1;Parent=MS.gene22515.t1
chr7.3 CDS 540535 541748 540535 ID=cds.MS.gene22515.t1;Parent=MS.gene22515.t1
chr7.3 exon 541915 542132 541915 ID=MS.gene22515.t1.exon2;Parent=MS.gene22515.t1
chr7.3 CDS 541915 542132 541915 ID=cds.MS.gene22515.t1;Parent=MS.gene22515.t1
Gene Sequence

>MS.gene22515

CATAGACATGGATAAAGAAAGACTAACGGCGGAGATGAATTTCAAAGATTCAACCTCCGCGGTTATCAAAATCCGACGACGTTTACCGGATTTTCTACAGACGGTGAAACTCAAGTACGTAAAGTTAGGTTATGGCTATTCATGCAACGCCACAACCATTCTCATTTTCACACTCGTCGTCCCTCTCTTACTCTTCCTCACCGTTCAGTTCCAACTCACCGATCTAAAACTCCACCGTCTCTCACAACTCTTGCTCGAACACACCGTCCAACTTGACACCGATACAGCCATCGGTTCAATTGTTCTACTCTTCCTCTTCGGTCTGTACTACGCGAAACGATCCCCACCAATTTACCTGGTGGATTTTGCGTGCTACAAACCGGAGAAAGAGCGGAAAATAGCAGTGGAATCTTTTGTTAAAATGTTAGAAGATAGTGGCGAATTCGGAGAAGAAACACTTCAATTTCAACGCCGCATTTTGTCAAGAGCAGGTTTAGGCGACGAAACTTCCTTGCCAAATGGAATAATGTCAAGTCCACCAAATCTATGCATGAAAGAAGCGCGTTTAGAAGCAGAAGCTGTAATGTTTGGATCATTAGACGCACTTTTTGCTAAAACAGGCGTTAATCCAAGAGACATTGACATTCTTGTTGTAAATTGTAGTTTGTTTAACCCAACTCCTTCACTTTCTGCAATGATTGTGAACCACTACAAGCTAAGAACAAACATTAAAAGCTATAATCTCGGAGGTATGGGTTGTAGTGCTGGTCTTATTTCAATTGATCTTGCCAAAGATCTTTTGAAAGCAAATCCAAATTCCTACGCGGTGGTCTTGAGCACGGAGAATTTAACCCTCAATTGGTACTTTGGAAATGATCGTTCAATGCTTCTTTCTAATTGCATTTTCCGAATGGGTGGCGCCGCTATACTTCTTTCCAATAAGTCCTCTGATCTAACCCGGTCCAAATACAAGCTGGTTCATACAGTTAGAACCCATAAAGGAGCTGATGACAAGAACTATAACTGTGTTTACCAGAAAGAAGACGAGACAGGGAAAGTTGGTGTTTCATTGGCTAGAGAATTAATGGCTGTTGCAGGAGATGCTTTGAAAACAAACATAACAACGTTGGGTCCATTGGTTTTGCCATTTTCTGAACAGTTCATGTTTTTTGTGTCTTTGGTTAAAAGGAAGTTGCTGCTGAAGAAAGGTTCAGGTGTGAAACCTTATATACCAGATTTCAAATTAGCTTTTGAACATTTCTGTATTCATGCTGGTGGAAGAGCTGTTCTTGATGAGTTGCAGAAGAATCTCCAACTCAGTGAGTGGCATATGGAACCGTCTCGGATGACTCTGCATAGATTTGGAAACACTTCAAGCAGTTCTCTATGGTATGAACTGGCTTATACTGAGGCAAAGGGCCGGGTTGCAAAAGGGGATCGGGTCTGGCAGATTGCATTCGGGTCGGGCTTTAAGTGTAACAGTGCTGTGTGGAAGGCCGTGCGCGACTTGCCCGTCGTCGGGGATTGGCGGGGCAACCCCTGGGATGACTCTGTTGATAAGTATCCCGTCAGCGTCCCCTGTTTCTGTTGCTTCTTAA
Protein sequence

>MS.gene22515.t1

HRHGURKTNGGDEFQRFNLRGYQNPTTFTGFSTDGETQVRKVRLWLFMQRHNHSHFHTRRPSLTLPHRSVPTHRSKTPPSLTTLARTHRPTUHRYSHRFNCSTLPLRSVLRETIPTNLPGGFCVLQTGERAENSSGIFCUNVRRUWRIRRRNTSISTPHFVKSRFRRRNFLAKWNNVKSTKSMHERSAFRSRSCNVWIIRRTFCUNRRUSKRHUHSCCKLUFVUPNSFTFCNDCEPLQAKNKHUKLUSRRYGLUCWSYFNUSCQRSFESKSKFLRGGLEHGEFNPQLVLWKUSFNASFULHFPNGWRRYTSFQUVLUSNPVQIQAGSYSUNPURSUUQELULCLPERRRDRESWCFIGURINGCCRRCFENKHNNVGSIGFAIFUTVHVFCVFGUKEVAAEERFSSLWYELAYTEAKGRVAKGDRVWQIAFGSGFKCNSAVWKAVRDLPVVGDWRGNPWDDSVDKYPVSVPCFCCFL