AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22743

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22743.t1 MTR_7g118170 98.134 536 8 1 1 536 1 534 0.0 1085
MS.gene22743.t1 MTR_1g076430 64.741 502 166 5 30 531 17 507 0.0 672
MS.gene22743.t1 MTR_1g041085 65.139 502 164 5 30 531 23 513 0.0 664
MS.gene22743.t1 MTR_2g054940 65.269 501 163 4 31 531 17 506 0.0 663
MS.gene22743.t1 MTR_4g113280 62.874 501 164 4 31 531 6 484 0.0 637
MS.gene22743.t1 MTR_4g070270 60.792 505 184 5 27 530 11 502 0.0 631
MS.gene22743.t1 MTR_7g081695 62.800 500 157 7 31 527 17 490 0.0 619
MS.gene22743.t1 MTR_3g105550 55.664 512 212 7 27 530 32 536 0.0 596
MS.gene22743.t1 MTR_1g110930 57.800 500 197 6 33 531 5 491 0.0 592
MS.gene22743.t1 MTR_5g056510 57.058 503 196 6 33 531 5 491 0.0 576
MS.gene22743.t1 MTR_7g118160 61.822 461 128 4 70 528 3 417 0.0 570
MS.gene22743.t1 MTR_4g054630 54.390 467 205 4 67 532 9 468 0.0 521
MS.gene22743.t1 MTR_4g054415 57.882 425 173 3 109 532 8 427 0.0 520
MS.gene22743.t1 MTR_6g074880 56.481 432 177 4 100 530 47 468 1.61e-179 513
MS.gene22743.t1 MTR_6g032965 58.312 391 155 3 146 533 2 387 4.86e-166 476
MS.gene22743.t1 MTR_2g013740 49.293 495 228 5 27 519 27 500 7.53e-166 480
MS.gene22743.t1 MTR_2g007020 51.923 416 192 4 118 532 45 453 1.24e-155 452
MS.gene22743.t1 MTR_2g436480 48.605 430 209 5 105 530 30 451 9.88e-148 432
MS.gene22743.t1 MTR_1g102290 46.229 411 217 2 118 528 78 484 2.16e-133 397
MS.gene22743.t1 MTR_3g072940 45.476 420 218 5 114 530 74 485 2.94e-127 381
MS.gene22743.t1 MTR_3g072980 45.476 420 218 5 114 530 74 485 3.04e-127 381
MS.gene22743.t1 MTR_4g062270 43.256 430 225 7 106 527 15 433 3.53e-123 369
MS.gene22743.t1 MTR_2g096210 43.237 451 233 8 105 536 4 450 6.88e-123 369
MS.gene22743.t1 MTR_4g062170 42.661 436 227 6 106 527 15 441 9.87e-120 361
MS.gene22743.t1 MTR_4g062190 41.972 436 231 5 106 527 15 442 5.49e-119 359
MS.gene22743.t1 MTR_6g051440 45.631 412 213 5 121 529 80 483 1.48e-118 358
MS.gene22743.t1 MTR_1g115560 40.455 440 238 5 107 527 17 451 6.42e-118 357
MS.gene22743.t1 MTR_4g054260 53.992 263 98 4 271 532 87 327 4.10e-93 287
MS.gene22743.t1 MTR_4g053905 58.434 166 64 2 367 532 1 161 1.24e-63 205
MS.gene22743.t1 MTR_4g054265 40.708 113 67 0 107 219 6 118 1.26e-24 99.4
MS.gene22743.t1 MTR_4g078730 26.102 295 178 12 206 495 120 379 2.17e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22743.t1 AT1G01120 73.157 529 133 5 4 530 5 526 0.0 800
MS.gene22743.t1 AT2G26640 64.881 504 160 5 31 531 15 504 0.0 673
MS.gene22743.t1 AT1G04220 64.103 507 166 7 31 531 20 516 0.0 662
MS.gene22743.t1 AT1G19440 60.396 505 186 5 27 530 23 514 0.0 627
MS.gene22743.t1 AT5G43760 63.380 497 169 5 39 531 34 521 0.0 622
MS.gene22743.t1 AT2G16280 60.355 507 177 9 30 530 22 510 0.0 616
MS.gene22743.t1 AT2G26250 54.753 526 204 7 27 528 33 548 0.0 586
MS.gene22743.t1 AT1G68530 56.151 504 199 7 33 531 6 492 0.0 582
MS.gene22743.t1 AT4G34510 58.149 497 186 8 37 530 8 485 0.0 578
MS.gene22743.t1 AT2G46720 62.471 429 158 1 102 530 40 465 0.0 577
MS.gene22743.t1 AT1G25450 56.112 499 207 5 33 531 1 487 0.0 571
MS.gene22743.t1 AT3G10280 61.305 429 156 2 102 530 40 458 0.0 559
MS.gene22743.t1 AT2G15090 51.660 482 219 3 59 532 6 481 0.0 522
MS.gene22743.t1 AT4G34250 56.787 442 174 5 104 534 56 491 0.0 519
MS.gene22743.t1 AT4G34520 56.787 442 171 5 104 530 54 490 1.81e-179 515
MS.gene22743.t1 AT1G71160 50.116 431 206 3 106 535 31 453 5.12e-156 453
MS.gene22743.t1 AT5G49070 46.405 459 234 6 82 533 5 458 3.92e-147 431
MS.gene22743.t1 AT3G52160 45.763 413 188 3 108 520 74 450 2.78e-132 392
MS.gene22743.t1 AT3G52160 47.978 371 157 3 150 520 3 337 2.34e-123 365
MS.gene22743.t1 AT1G68530 47.244 381 185 5 33 408 6 375 4.19e-122 363
MS.gene22743.t1 AT2G28630 41.958 429 237 4 110 527 19 446 3.94e-121 365
MS.gene22743.t1 AT2G28630 41.958 429 237 4 110 527 19 446 3.94e-121 365
MS.gene22743.t1 AT5G04530 41.355 428 237 6 107 527 17 437 3.37e-117 354
MS.gene22743.t1 AT1G07720 42.991 428 223 6 116 527 25 447 2.66e-116 352
MS.gene22743.t1 AT1G07720 42.991 428 223 6 116 527 68 490 4.26e-116 353
MS.gene22743.t1 AT5G49070 42.071 309 173 3 82 384 5 313 1.63e-82 260

Find 135 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGTCTCGTCTTCTTTC+TGG 0.110532 7.4:-557660 None:intergenic
TCTTGTCATCAGCTCCTTTA+TGG 0.143442 7.4:-557621 None:intergenic
TGTATGAACCAGCTTGTATT+TGG 0.221689 7.4:-557590 None:intergenic
AAGACCACCGCGTAGGAATT+TGG 0.226830 7.4:-557439 None:intergenic
TTCATTGGCTAGAGAATTAA+TGG 0.254813 7.4:+557693 MS.gene22743:CDS
AACATGAACTGTTCAGAAAA+TGG 0.259600 7.4:-557772 None:intergenic
CAAAATCCGACGACGTTTAC+CGG 0.274993 7.4:+556694 MS.gene22743:CDS
GGTTCGATCAGAGGACTTAT+TGG 0.284693 7.4:-557563 None:intergenic
CTTGTCATCAGCTCCTTTAT+GGG 0.289862 7.4:-557620 None:intergenic
AATTTGAAATCTGGTATATA+AGG 0.292144 7.4:-557850 None:intergenic
ATTTGCTTTCAAAAGATCTT+TGG 0.295644 7.4:-557416 None:intergenic
GCAAAATCCACCAGGTAAAT+TGG 0.298744 7.4:-556974 None:intergenic
GGGATTGGCGGGGCAACCCC+TGG 0.302657 7.4:+558148 MS.gene22743:CDS
GAAGCAGAAGCTGTAATGTT+TGG 0.314657 7.4:+557196 MS.gene22743:CDS
TCAAAAGCTAATTTGAAATC+TGG 0.331655 7.4:-557859 None:intergenic
CGGGTCTGGCAGATTGCATT+CGG 0.336102 7.4:+558066 MS.gene22743:CDS
TTGCAAAAGGGGATCGGGTC+TGG 0.336451 7.4:+558052 MS.gene22743:CDS
TGCGCGACTTGCCCGTCGTC+GGG 0.341272 7.4:+558127 MS.gene22743:CDS
GCCCGTCGTCGGGGATTGGC+GGG 0.352548 7.4:+558137 MS.gene22743:CDS
TTAACCCTCAATTGGTACTT+TGG 0.358565 7.4:+557475 MS.gene22743:CDS
GGACTTGACATTATTCCATT+TGG 0.369284 7.4:-557142 None:intergenic
CGGATGACTCTGCATAGATT+TGG 0.389216 7.4:+557970 MS.gene22743:CDS
GCTTCTTTCATGCATAGATT+TGG 0.389782 7.4:-557166 None:intergenic
GCAAAGGGCCGGGTTGCAAA+AGG 0.390952 7.4:+558039 MS.gene22743:CDS
AGAGAATTAATGGCTGTTGC+AGG 0.392253 7.4:+557703 MS.gene22743:CDS
GTTAAAATGTTAGAAGATAG+TGG 0.394444 7.4:+557043 MS.gene22743:CDS
AGGGAAAGTTGGTGTTTCAT+TGG 0.396299 7.4:+557678 MS.gene22743:CDS
ACACTTCAAGCAGTTCTCTA+TGG 0.396346 7.4:+557995 MS.gene22743:CDS
ACTTGCCCGTCGTCGGGGAT+TGG 0.409147 7.4:+558133 MS.gene22743:CDS
AAGCTATAATCTCGGAGGTA+TGG 0.411775 7.4:+557360 MS.gene22743:CDS
AAAACAAACATAACAACGTT+GGG 0.411988 7.4:+557736 MS.gene22743:CDS
TTAGAAGATAGTGGCGAATT+CGG 0.414252 7.4:+557052 MS.gene22743:CDS
AAACTCAAGTACGTAAAGTT+AGG 0.423051 7.4:+556734 MS.gene22743:CDS
CATAACAACGTTGGGTCCAT+TGG 0.424394 7.4:+557744 MS.gene22743:CDS
CCAACTCAGTGAGTGGCATA+TGG 0.428746 7.4:+557939 MS.gene22743:CDS
TGGCAGATTGCATTCGGGTC+GGG 0.430711 7.4:+558072 MS.gene22743:CDS
GGAGGTATGGGTTGTAGTGC+TGG 0.432856 7.4:+557373 MS.gene22743:CDS
CGGAGAATTTAACCCTCAAT+TGG 0.433036 7.4:+557467 MS.gene22743:CDS
ATTGTTCTACTCTTCCTCTT+CGG 0.433972 7.4:+556926 MS.gene22743:CDS
TTGCTGCTGAAGAAAGGTTC+AGG 0.437231 7.4:+557820 MS.gene22743:CDS
CCGGGTTGCAAAAGGGGATC+GGG 0.444082 7.4:+558047 MS.gene22743:CDS
CTGGCAGATTGCATTCGGGT+CGG 0.444799 7.4:+558071 MS.gene22743:CDS
GCCGGGTTGCAAAAGGGGAT+CGG 0.445235 7.4:+558046 MS.gene22743:CDS
CAGTTCTCTATGGTATGAAC+TGG 0.451448 7.4:+558005 MS.gene22743:CDS
CATACAGTTAGAACCCATAA+AGG 0.451862 7.4:+557607 MS.gene22743:CDS
CAAAGGGCCGGGTTGCAAAA+GGG 0.466708 7.4:+558040 MS.gene22743:CDS
ACAAGAATGTCAATGTCTCT+TGG 0.469200 7.4:-557256 None:intergenic
GTATAGCGGCGCCACCCATT+CGG 0.470845 7.4:-557534 None:intergenic
TCTTTCATGCATAGATTTGG+TGG 0.474801 7.4:-557163 None:intergenic
TTGCAGAAAGTGAAGGAGTT+GGG 0.476301 7.4:-557300 None:intergenic
GTGCGCGACTTGCCCGTCGT+CGG 0.478086 7.4:+558126 MS.gene22743:CDS
CATTTCCAAAGTACCAATTG+AGG 0.478571 7.4:-557480 None:intergenic
GGATTGGCGGGGCAACCCCT+GGG 0.483271 7.4:+558149 MS.gene22743:CDS
TGCCCGTCGTCGGGGATTGG+CGG 0.485832 7.4:+558136 MS.gene22743:CDS
AGCTATAATCTCGGAGGTAT+GGG 0.488082 7.4:+557361 MS.gene22743:CDS
GAACCAGCTTGTATTTGGAC+CGG 0.488419 7.4:-557585 None:intergenic
AAGTACGTAAAGTTAGGTTA+TGG 0.495016 7.4:+556740 MS.gene22743:CDS
GAACATTTCTGTATTCATGC+TGG 0.495485 7.4:+557880 MS.gene22743:CDS
CCCCGCCAATCCCCGACGAC+GGG 0.497979 7.4:-558138 None:intergenic
TTCACCGTCTGTAGAAAATC+CGG 0.498288 7.4:-556713 None:intergenic
AGGAAGTTGCTGCTGAAGAA+AGG 0.501320 7.4:+557814 MS.gene22743:CDS
AAAGGGCCGGGTTGCAAAAG+GGG 0.505317 7.4:+558041 MS.gene22743:CDS
ATTTCCAAAGTACCAATTGA+GGG 0.506557 7.4:-557479 None:intergenic
GGGTCTGGCAGATTGCATTC+GGG 0.514216 7.4:+558067 MS.gene22743:CDS
AATTGAACCGATGGCTGTAT+CGG 0.514947 7.4:-556906 None:intergenic
GACGAAACTTCCTTGCCAAA+TGG 0.515853 7.4:+557127 MS.gene22743:CDS
TTATACTGAGGCAAAGGGCC+GGG 0.516750 7.4:+558029 MS.gene22743:CDS
GAAAACAAACATAACAACGT+TGG 0.519196 7.4:+557735 MS.gene22743:CDS
TGTTTGTTCTTAGCTTGTAG+TGG 0.521220 7.4:-557333 None:intergenic
ACAGAAACAGGGACGCTGAC+GGG 0.521485 7.4:-558192 None:intergenic
AACATTAAAAGCTATAATCT+CGG 0.522267 7.4:+557352 MS.gene22743:CDS
CTTATACTGAGGCAAAGGGC+CGG 0.522593 7.4:+558028 MS.gene22743:CDS
CCCGTCGTCGGGGATTGGCG+GGG 0.523629 7.4:+558138 MS.gene22743:CDS
TACCAGAAAGAAGACGAGAC+AGG 0.524700 7.4:+557658 MS.gene22743:CDS
AATCCACCAGGTAAATTGGT+GGG 0.541242 7.4:-556970 None:intergenic
ATTGCAGAAAGTGAAGGAGT+TGG 0.552174 7.4:-557301 None:intergenic
GAAGACGAGACAGGGAAAGT+TGG 0.555142 7.4:+557667 MS.gene22743:CDS
TCAGAAAATGGCAAAACCAA+TGG 0.557047 7.4:-557760 None:intergenic
GCCCCGCCAATCCCCGACGA+CGG 0.559697 7.4:-558139 None:intergenic
ACGATCCCCACCAATTTACC+TGG 0.560276 7.4:+556964 MS.gene22743:CDS
TTTGGACCGGGTTCGATCAG+AGG 0.562259 7.4:-557572 None:intergenic
CCATATGCCACTCACTGAGT+TGG 0.569030 7.4:-557939 None:intergenic
GCAAGAGTTGTGAGAGACGG+TGG 0.571620 7.4:-556859 None:intergenic
TGGCTGTATCGGTGTCAAGT+TGG 0.573119 7.4:-556895 None:intergenic
GACGACGGGCAAGTCGCGCA+CGG 0.573305 7.4:-558124 None:intergenic
ACTGGCTTATACTGAGGCAA+AGG 0.574125 7.4:+558023 MS.gene22743:CDS
AACCAGCTTGTATTTGGACC+GGG 0.576851 7.4:-557584 None:intergenic
CTTATCAACAGAGTCATCCC+AGG 0.582184 7.4:-558166 None:intergenic
ATAAGTCCTCTGATCGAACC+CGG 0.585371 7.4:+557566 MS.gene22743:CDS
ATCCACCAGGTAAATTGGTG+GGG 0.585932 7.4:-556969 None:intergenic
AACCCGGTCCAAATACAAGC+TGG 0.588921 7.4:+557582 MS.gene22743:CDS
TGACATTATTCCATTTGGCA+AGG 0.590067 7.4:-557137 None:intergenic
CTGGCTTATACTGAGGCAAA+GGG 0.590480 7.4:+558024 MS.gene22743:CDS
CATTTCTGTATTCATGCTGG+TGG 0.590993 7.4:+557883 MS.gene22743:CDS
AACAGAAACAGGGACGCTGA+CGG 0.594758 7.4:-558193 None:intergenic
GAGTAGAACAATTGAACCGA+TGG 0.597177 7.4:-556915 None:intergenic
AAATCCACCAGGTAAATTGG+TGG 0.601519 7.4:-556971 None:intergenic
AACCTGCTCTTGACAAAATG+CGG 0.602486 7.4:-557099 None:intergenic
GTATGAACTGGCTTATACTG+AGG 0.604352 7.4:+558017 MS.gene22743:CDS
TGTGAAAATGAGAATGGTTG+TGG 0.605218 7.4:-556777 None:intergenic
ATCCCCACCAATTTACCTGG+TGG 0.607799 7.4:+556967 MS.gene22743:CDS
ATCGGTGAGTTGGAACTGAA+CGG 0.608509 7.4:-556828 None:intergenic
ACAATCATTGCAGAAAGTGA+AGG 0.609013 7.4:-557307 None:intergenic
ACCAGAAAGAAGACGAGACA+GGG 0.610150 7.4:+557659 MS.gene22743:CDS
GTGTAACAGTGCTGTGTGGA+AGG 0.610162 7.4:+558101 MS.gene22743:CDS
TTTAAGAAGCAACAGAAACA+GGG 0.614888 7.4:-558203 None:intergenic
CGGTGAGGAAGAGTAAGAGA+GGG 0.614974 7.4:-556808 None:intergenic
GCGCGACTTGCCCGTCGTCG+GGG 0.623964 7.4:+558128 MS.gene22743:CDS
CGAGCAAGAGTTGTGAGAGA+CGG 0.627180 7.4:-556862 None:intergenic
GACGAGTGTGAAAATGAGAA+TGG 0.628164 7.4:-556783 None:intergenic
ACGGTGAGGAAGAGTAAGAG+AGG 0.633167 7.4:-556809 None:intergenic
CTACGCGGTGGTCTTGAGCA+CGG 0.635511 7.4:+557447 MS.gene22743:CDS
GAAAATCCGGTAAACGTCGT+CGG 0.635960 7.4:-556700 None:intergenic
GCTACAAACCGGAGAAAGAG+CGG 0.638965 7.4:+556999 MS.gene22743:CDS
AGTGGCATATGGAACCGTCT+CGG 0.641136 7.4:+557950 MS.gene22743:CDS
TGTAGCACGCAAAATCCACC+AGG 0.643428 7.4:-556982 None:intergenic
CTTGACACCGATACAGCCAT+CGG 0.644102 7.4:+556899 MS.gene22743:CDS
GGGTCGCAACAGCATAGACA+TGG 0.648812 7.4:+556616 MS.gene22743:CDS
TTATCAACAGAGTCATCCCA+GGG 0.649243 7.4:-558165 None:intergenic
AGAATCTCCAACTCAGTGAG+TGG 0.655119 7.4:+557932 MS.gene22743:CDS
TGTATCGGTGTCAAGTTGGA+CGG 0.658526 7.4:-556891 None:intergenic
CTATGCAGAGTCATCCGAGA+CGG 0.659543 7.4:-557964 None:intergenic
CTTATTGGAAAGAAGTATAG+CGG 0.661996 7.4:-557548 None:intergenic
CATGGATAAAGAAAGACTAA+CGG 0.662335 7.4:+556634 MS.gene22743:CDS
AAATCCAAATTCCTACGCGG+TGG 0.667104 7.4:+557435 MS.gene22743:CDS
GAAAGAGCGGAAAATAGCAG+TGG 0.668978 7.4:+557012 MS.gene22743:CDS
CGTGCTCAAGACCACCGCGT+AGG 0.672026 7.4:-557446 None:intergenic
ATTAAAAGCTATAATCTCGG+AGG 0.675727 7.4:+557355 MS.gene22743:CDS
TCGCGTAGTACAGACCGAAG+AGG 0.689821 7.4:-556940 None:intergenic
TTTCAAAGATTCAACCTCCG+CGG 0.705846 7.4:+556667 MS.gene22743:CDS
TGAGTTGGAACTGAACGGTG+AGG 0.720930 7.4:-556823 None:intergenic
TTAAGTGTAACAGTGCTGTG+TGG 0.723760 7.4:+558097 MS.gene22743:CDS
GGATAAAGAAAGACTAACGG+CGG 0.724763 7.4:+556637 MS.gene22743:CDS
AGCAAATCCAAATTCCTACG+CGG 0.758527 7.4:+557432 MS.gene22743:CDS
TATCAACAGAGTCATCCCAG+GGG 0.818112 7.4:-558164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATTAAAAGCTATAATCT+CGG + chr7.4:557352-557371 MS.gene22743:CDS 20.0%
!!! AATTTGAAATCTGGTATATA+AGG - chr7.4:557853-557872 None:intergenic 20.0%
! AAAACAAACATAACAACGTT+GGG + chr7.4:557736-557755 MS.gene22743:CDS 25.0%
! ATTTGCTTTCAAAAGATCTT+TGG - chr7.4:557419-557438 None:intergenic 25.0%
! GTTAAAATGTTAGAAGATAG+TGG + chr7.4:557043-557062 MS.gene22743:CDS 25.0%
!! TCAAAAGCTAATTTGAAATC+TGG - chr7.4:557862-557881 None:intergenic 25.0%
!! TTTTTGTGTCTTTGGTTAAA+AGG + chr7.4:557794-557813 MS.gene22743:CDS 25.0%
AAACTCAAGTACGTAAAGTT+AGG + chr7.4:556734-556753 MS.gene22743:CDS 30.0%
AACATGAACTGTTCAGAAAA+TGG - chr7.4:557775-557794 None:intergenic 30.0%
AAGTACGTAAAGTTAGGTTA+TGG + chr7.4:556740-556759 MS.gene22743:CDS 30.0%
ATTAAAAGCTATAATCTCGG+AGG + chr7.4:557355-557374 MS.gene22743:CDS 30.0%
CATGGATAAAGAAAGACTAA+CGG + chr7.4:556634-556653 MS.gene22743:CDS 30.0%
GAAAACAAACATAACAACGT+TGG + chr7.4:557735-557754 MS.gene22743:CDS 30.0%
! ATTTCCAAAGTACCAATTGA+GGG - chr7.4:557482-557501 None:intergenic 30.0%
! CTTATTGGAAAGAAGTATAG+CGG - chr7.4:557551-557570 None:intergenic 30.0%
! TCTAATTGCATTTTCCGAAT+GGG + chr7.4:557520-557539 MS.gene22743:CDS 30.0%
! TTCATTGGCTAGAGAATTAA+TGG + chr7.4:557693-557712 MS.gene22743:CDS 30.0%
! TTCTAATTGCATTTTCCGAA+TGG + chr7.4:557519-557538 MS.gene22743:CDS 30.0%
!!! GTTCATGTTTTTTGTGTCTT+TGG + chr7.4:557786-557805 MS.gene22743:CDS 30.0%
ACAAGAATGTCAATGTCTCT+TGG - chr7.4:557259-557278 None:intergenic 35.0%
ACAATCATTGCAGAAAGTGA+AGG - chr7.4:557310-557329 None:intergenic 35.0%
ATTGTTCTACTCTTCCTCTT+CGG + chr7.4:556926-556945 MS.gene22743:CDS 35.0%
CATACAGTTAGAACCCATAA+AGG + chr7.4:557607-557626 MS.gene22743:CDS 35.0%
GAACATTTCTGTATTCATGC+TGG + chr7.4:557880-557899 MS.gene22743:CDS 35.0%
GCTTCTTTCATGCATAGATT+TGG - chr7.4:557169-557188 None:intergenic 35.0%
GGACTTGACATTATTCCATT+TGG - chr7.4:557145-557164 None:intergenic 35.0%
TCAGAAAATGGCAAAACCAA+TGG - chr7.4:557763-557782 None:intergenic 35.0%
TCTTTCATGCATAGATTTGG+TGG - chr7.4:557166-557185 None:intergenic 35.0%
TGACATTATTCCATTTGGCA+AGG - chr7.4:557140-557159 None:intergenic 35.0%
TGTATGAACCAGCTTGTATT+TGG - chr7.4:557593-557612 None:intergenic 35.0%
TGTGAAAATGAGAATGGTTG+TGG - chr7.4:556780-556799 None:intergenic 35.0%
TTAACCCTCAATTGGTACTT+TGG + chr7.4:557475-557494 MS.gene22743:CDS 35.0%
! ATTTTGTCAAGAGCAGGTTT+AGG + chr7.4:557103-557122 MS.gene22743:CDS 35.0%
! CATTTCCAAAGTACCAATTG+AGG - chr7.4:557483-557502 None:intergenic 35.0%
! TTAGAAGATAGTGGCGAATT+CGG + chr7.4:557052-557071 MS.gene22743:CDS 35.0%
! TTTACCGGATTTTCTACAGA+CGG + chr7.4:556709-556728 MS.gene22743:CDS 35.0%
!! TGTTTGTTCTTAGCTTGTAG+TGG - chr7.4:557336-557355 None:intergenic 35.0%
AAATCCACCAGGTAAATTGG+TGG - chr7.4:556974-556993 None:intergenic 40.0%
AACCTGCTCTTGACAAAATG+CGG - chr7.4:557102-557121 None:intergenic 40.0%
AAGCTATAATCTCGGAGGTA+TGG + chr7.4:557360-557379 MS.gene22743:CDS 40.0%
AATCCACCAGGTAAATTGGT+GGG - chr7.4:556973-556992 None:intergenic 40.0%
AATTGAACCGATGGCTGTAT+CGG - chr7.4:556909-556928 None:intergenic 40.0%
ACACTTCAAGCAGTTCTCTA+TGG + chr7.4:557995-558014 MS.gene22743:CDS 40.0%
AGAGAATTAATGGCTGTTGC+AGG + chr7.4:557703-557722 MS.gene22743:CDS 40.0%
AGCAAATCCAAATTCCTACG+CGG + chr7.4:557432-557451 MS.gene22743:CDS 40.0%
ATTGCAGAAAGTGAAGGAGT+TGG - chr7.4:557304-557323 None:intergenic 40.0%
CAGTTCTCTATGGTATGAAC+TGG + chr7.4:558005-558024 MS.gene22743:CDS 40.0%
CATTTCTGTATTCATGCTGG+TGG + chr7.4:557883-557902 MS.gene22743:CDS 40.0%
CGGAGAATTTAACCCTCAAT+TGG + chr7.4:557467-557486 MS.gene22743:CDS 40.0%
CTTGTCATCAGCTCCTTTAT+GGG - chr7.4:557623-557642 None:intergenic 40.0%
GAAGCAGAAGCTGTAATGTT+TGG + chr7.4:557196-557215 MS.gene22743:CDS 40.0%
GACGAGTGTGAAAATGAGAA+TGG - chr7.4:556786-556805 None:intergenic 40.0%
GAGTAGAACAATTGAACCGA+TGG - chr7.4:556918-556937 None:intergenic 40.0%
GCAAAATCCACCAGGTAAAT+TGG - chr7.4:556977-556996 None:intergenic 40.0%
GGATAAAGAAAGACTAACGG+CGG + chr7.4:556637-556656 MS.gene22743:CDS 40.0%
TCTTGTCATCAGCTCCTTTA+TGG - chr7.4:557624-557643 None:intergenic 40.0%
TTAAGTGTAACAGTGCTGTG+TGG + chr7.4:558097-558116 MS.gene22743:CDS 40.0%
TTATCAACAGAGTCATCCCA+GGG - chr7.4:558168-558187 None:intergenic 40.0%
TTCACCGTCTGTAGAAAATC+CGG - chr7.4:556716-556735 None:intergenic 40.0%
TTGCAGAAAGTGAAGGAGTT+GGG - chr7.4:557303-557322 None:intergenic 40.0%
TTTCAAAGATTCAACCTCCG+CGG + chr7.4:556667-556686 MS.gene22743:CDS 40.0%
! AATTGCATTTTCCGAATGGG+TGG + chr7.4:557523-557542 MS.gene22743:CDS 40.0%
! AGCTATAATCTCGGAGGTAT+GGG + chr7.4:557361-557380 MS.gene22743:CDS 40.0%
! GTATGAACTGGCTTATACTG+AGG + chr7.4:558017-558036 MS.gene22743:CDS 40.0%
!! AGGGAAAGTTGGTGTTTCAT+TGG + chr7.4:557678-557697 MS.gene22743:CDS 40.0%
!! GACGCACTTTTTGCTAAAAC+AGG + chr7.4:557226-557245 MS.gene22743:CDS 40.0%
AAATCCAAATTCCTACGCGG+TGG + chr7.4:557435-557454 MS.gene22743:CDS 45.0%
AACCAGCTTGTATTTGGACC+GGG - chr7.4:557587-557606 None:intergenic 45.0%
ACCAGAAAGAAGACGAGACA+GGG + chr7.4:557659-557678 MS.gene22743:CDS 45.0%
AGAATCTCCAACTCAGTGAG+TGG + chr7.4:557932-557951 MS.gene22743:CDS 45.0%
AGGAAGTTGCTGCTGAAGAA+AGG + chr7.4:557814-557833 MS.gene22743:CDS 45.0%
ATAAGTCCTCTGATCGAACC+CGG + chr7.4:557566-557585 MS.gene22743:CDS 45.0%
ATCCACCAGGTAAATTGGTG+GGG - chr7.4:556972-556991 None:intergenic 45.0%
CAAAATCCGACGACGTTTAC+CGG + chr7.4:556694-556713 MS.gene22743:CDS 45.0%
CATAACAACGTTGGGTCCAT+TGG + chr7.4:557744-557763 MS.gene22743:CDS 45.0%
CGGATGACTCTGCATAGATT+TGG + chr7.4:557970-557989 MS.gene22743:CDS 45.0%
CTGGCTTATACTGAGGCAAA+GGG + chr7.4:558024-558043 MS.gene22743:CDS 45.0%
CTTATCAACAGAGTCATCCC+AGG - chr7.4:558169-558188 None:intergenic 45.0%
GAAAATCCGGTAAACGTCGT+CGG - chr7.4:556703-556722 None:intergenic 45.0%
GAAAGAGCGGAAAATAGCAG+TGG + chr7.4:557012-557031 MS.gene22743:CDS 45.0%
GAACCAGCTTGTATTTGGAC+CGG - chr7.4:557588-557607 None:intergenic 45.0%
GACGAAACTTCCTTGCCAAA+TGG + chr7.4:557127-557146 MS.gene22743:CDS 45.0%
GGTTCGATCAGAGGACTTAT+TGG - chr7.4:557566-557585 None:intergenic 45.0%
TACCAGAAAGAAGACGAGAC+AGG + chr7.4:557658-557677 MS.gene22743:CDS 45.0%
TATCAACAGAGTCATCCCAG+GGG - chr7.4:558167-558186 None:intergenic 45.0%
TTGCTGCTGAAGAAAGGTTC+AGG + chr7.4:557820-557839 MS.gene22743:CDS 45.0%
! ACTGGCTTATACTGAGGCAA+AGG + chr7.4:558023-558042 MS.gene22743:CDS 45.0%
! ATCGGTGAGTTGGAACTGAA+CGG - chr7.4:556831-556850 None:intergenic 45.0%
! GGATTTTGCGTGCTACAAAC+CGG + chr7.4:556988-557007 MS.gene22743:CDS 45.0%
!! GCTATTTTCCGCTCTTTCTC+CGG - chr7.4:557010-557029 None:intergenic 45.0%
!! TCGTCGGATTTTGATAACCG+CGG - chr7.4:556687-556706 None:intergenic 45.0%
!! TGTATCGGTGTCAAGTTGGA+CGG - chr7.4:556894-556913 None:intergenic 45.0%
!!! GAGACGGTGGAGTTTTAGAT+CGG - chr7.4:556849-556868 None:intergenic 45.0%
!!! GGAGTTTTAGATCGGTGAGT+TGG - chr7.4:556841-556860 None:intergenic 45.0%
AACAGAAACAGGGACGCTGA+CGG - chr7.4:558196-558215 None:intergenic 50.0%
AACCCGGTCCAAATACAAGC+TGG + chr7.4:557582-557601 MS.gene22743:CDS 50.0%
AAGACCACCGCGTAGGAATT+TGG - chr7.4:557442-557461 None:intergenic 50.0%
ACGATCCCCACCAATTTACC+TGG + chr7.4:556964-556983 MS.gene22743:CDS 50.0%
ACGGTGAGGAAGAGTAAGAG+AGG - chr7.4:556812-556831 None:intergenic 50.0%
AGTGGCATATGGAACCGTCT+CGG + chr7.4:557950-557969 MS.gene22743:CDS 50.0%
ATCCCCACCAATTTACCTGG+TGG + chr7.4:556967-556986 MS.gene22743:CDS 50.0%
CCAACTCAGTGAGTGGCATA+TGG + chr7.4:557939-557958 MS.gene22743:CDS 50.0%
CCATATGCCACTCACTGAGT+TGG - chr7.4:557942-557961 None:intergenic 50.0%
CGAGCAAGAGTTGTGAGAGA+CGG - chr7.4:556865-556884 None:intergenic 50.0%
CGGTGAGGAAGAGTAAGAGA+GGG - chr7.4:556811-556830 None:intergenic 50.0%
CTATGCAGAGTCATCCGAGA+CGG - chr7.4:557967-557986 None:intergenic 50.0%
CTTATACTGAGGCAAAGGGC+CGG + chr7.4:558028-558047 MS.gene22743:CDS 50.0%
CTTGACACCGATACAGCCAT+CGG + chr7.4:556899-556918 MS.gene22743:CDS 50.0%
GAAGACGAGACAGGGAAAGT+TGG + chr7.4:557667-557686 MS.gene22743:CDS 50.0%
GCTACAAACCGGAGAAAGAG+CGG + chr7.4:556999-557018 MS.gene22743:CDS 50.0%
GTGTAACAGTGCTGTGTGGA+AGG + chr7.4:558101-558120 MS.gene22743:CDS 50.0%
TCCCTGTCTCGTCTTCTTTC+TGG - chr7.4:557663-557682 None:intergenic 50.0%
TGTAGCACGCAAAATCCACC+AGG - chr7.4:556985-557004 None:intergenic 50.0%
TTATACTGAGGCAAAGGGCC+GGG + chr7.4:558029-558048 MS.gene22743:CDS 50.0%
! TGAGTTGGAACTGAACGGTG+AGG - chr7.4:556826-556845 None:intergenic 50.0%
!! TCGGATTTTGATAACCGCGG+AGG - chr7.4:556684-556703 None:intergenic 50.0%
!! TGGCTGTATCGGTGTCAAGT+TGG - chr7.4:556898-556917 None:intergenic 50.0%
AAAGGGCCGGGTTGCAAAAG+GGG + chr7.4:558041-558060 MS.gene22743:CDS 55.0%
ACAGAAACAGGGACGCTGAC+GGG - chr7.4:558195-558214 None:intergenic 55.0%
CAAAGGGCCGGGTTGCAAAA+GGG + chr7.4:558040-558059 MS.gene22743:CDS 55.0%
CGGGTCTGGCAGATTGCATT+CGG + chr7.4:558066-558085 MS.gene22743:CDS 55.0%
CTGGCAGATTGCATTCGGGT+CGG + chr7.4:558071-558090 MS.gene22743:CDS 55.0%
GCAAGAGTTGTGAGAGACGG+TGG - chr7.4:556862-556881 None:intergenic 55.0%
GGGTCGCAACAGCATAGACA+TGG + chr7.4:556616-556635 MS.gene22743:CDS 55.0%
GGGTCTGGCAGATTGCATTC+GGG + chr7.4:558067-558086 MS.gene22743:CDS 55.0%
TCGCGTAGTACAGACCGAAG+AGG - chr7.4:556943-556962 None:intergenic 55.0%
TGGCAGATTGCATTCGGGTC+GGG + chr7.4:558072-558091 MS.gene22743:CDS 55.0%
TTGCAAAAGGGGATCGGGTC+TGG + chr7.4:558052-558071 MS.gene22743:CDS 55.0%
TTTGGACCGGGTTCGATCAG+AGG - chr7.4:557575-557594 None:intergenic 55.0%
! CGCCGCATTTTGTCAAGAGC+AGG + chr7.4:557097-557116 MS.gene22743:CDS 55.0%
! GGAGGTATGGGTTGTAGTGC+TGG + chr7.4:557373-557392 MS.gene22743:CDS 55.0%
CCGGGTTGCAAAAGGGGATC+GGG + chr7.4:558047-558066 MS.gene22743:CDS 60.0%
GCAAAGGGCCGGGTTGCAAA+AGG + chr7.4:558039-558058 MS.gene22743:CDS 60.0%
GCCGGGTTGCAAAAGGGGAT+CGG + chr7.4:558046-558065 MS.gene22743:CDS 60.0%
GTATAGCGGCGCCACCCATT+CGG - chr7.4:557537-557556 None:intergenic 60.0%
! CCCGATCCCCTTTTGCAACC+CGG - chr7.4:558050-558069 None:intergenic 60.0%
! CTACGCGGTGGTCTTGAGCA+CGG + chr7.4:557447-557466 MS.gene22743:CDS 60.0%
ACTTGCCCGTCGTCGGGGAT+TGG + chr7.4:558133-558152 MS.gene22743:CDS 65.0%
CGTGCTCAAGACCACCGCGT+AGG - chr7.4:557449-557468 None:intergenic 65.0%
GACGACGGGCAAGTCGCGCA+CGG - chr7.4:558127-558146 None:intergenic 70.0%
GTGCGCGACTTGCCCGTCGT+CGG + chr7.4:558126-558145 MS.gene22743:CDS 70.0%
TGCCCGTCGTCGGGGATTGG+CGG + chr7.4:558136-558155 MS.gene22743:CDS 70.0%
TGCGCGACTTGCCCGTCGTC+GGG + chr7.4:558127-558146 MS.gene22743:CDS 70.0%
! GGATTGGCGGGGCAACCCCT+GGG + chr7.4:558149-558168 MS.gene22743:CDS 70.0%
CCCCGCCAATCCCCGACGAC+GGG - chr7.4:558141-558160 None:intergenic 75.0%
GCCCCGCCAATCCCCGACGA+CGG - chr7.4:558142-558161 None:intergenic 75.0%
GCGCGACTTGCCCGTCGTCG+GGG + chr7.4:558128-558147 MS.gene22743:CDS 75.0%
! CCCGTCGTCGGGGATTGGCG+GGG + chr7.4:558138-558157 MS.gene22743:CDS 75.0%
! GCCCGTCGTCGGGGATTGGC+GGG + chr7.4:558137-558156 MS.gene22743:CDS 75.0%
! GGGATTGGCGGGGCAACCCC+TGG + chr7.4:558148-558167 MS.gene22743:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 556614 558224 556614 ID=MS.gene22743
chr7.4 mRNA 556614 558224 556614 ID=MS.gene22743.t1;Parent=MS.gene22743
chr7.4 exon 556614 558224 556614 ID=MS.gene22743.t1.exon1;Parent=MS.gene22743.t1
chr7.4 CDS 556614 558224 556614 ID=cds.MS.gene22743.t1;Parent=MS.gene22743.t1
Gene Sequence

>MS.gene22743

ATGGGTCGCAACAGCATAGACATGGATAAAGAAAGACTAACGGCGGAGATGAATTTCAAAGATTCAACCTCCGCGGTTATCAAAATCCGACGACGTTTACCGGATTTTCTACAGACGGTGAAACTCAAGTACGTAAAGTTAGGTTATGGCTATTCATGCAACGCCACAACCATTCTCATTTTCACACTCGTCGTCCCTCTCTTACTCTTCCTCACCGTTCAGTTCCAACTCACCGATCTAAAACTCCACCGTCTCTCACAACTCTTGCTCGAACACACCGTCCAACTTGACACCGATACAGCCATCGGTTCAATTGTTCTACTCTTCCTCTTCGGTCTGTACTACGCGAAACGATCCCCACCAATTTACCTGGTGGATTTTGCGTGCTACAAACCGGAGAAAGAGCGGAAAATAGCAGTGGAATCTTTTGTTAAAATGTTAGAAGATAGTGGCGAATTCGGAGAAGAAACACTTCAATTTCAACGCCGCATTTTGTCAAGAGCAGGTTTAGGCGACGAAACTTCCTTGCCAAATGGAATAATGTCAAGTCCACCAAATCTATGCATGAAAGAAGCGCGTTTAGAAGCAGAAGCTGTAATGTTTGGATCATTAGACGCACTTTTTGCTAAAACAGGCGTTAATCCAAGAGACATTGACATTCTTGTTGTAAATTGTAGTTTGTTTAACCCAACTCCTTCACTTTCTGCAATGATTGTGAACCACTACAAGCTAAGAACAAACATTAAAAGCTATAATCTCGGAGGTATGGGTTGTAGTGCTGGTCTTATTTCAATTGATCTTGCCAAAGATCTTTTGAAAGCAAATCCAAATTCCTACGCGGTGGTCTTGAGCACGGAGAATTTAACCCTCAATTGGTACTTTGGAAATGATCGTTCAATGCTTCTTTCTAATTGCATTTTCCGAATGGGTGGCGCCGCTATACTTCTTTCCAATAAGTCCTCTGATCGAACCCGGTCCAAATACAAGCTGGTTCATACAGTTAGAACCCATAAAGGAGCTGATGACAAGAACTATAACTGTGTTTACCAGAAAGAAGACGAGACAGGGAAAGTTGGTGTTTCATTGGCTAGAGAATTAATGGCTGTTGCAGGAGATGCTTTGAAAACAAACATAACAACGTTGGGTCCATTGGTTTTGCCATTTTCTGAACAGTTCATGTTTTTTGTGTCTTTGGTTAAAAGGAAGTTGCTGCTGAAGAAAGGTTCAGGTGTGAAACCTTATATACCAGATTTCAAATTAGCTTTTGAACATTTCTGTATTCATGCTGGTGGAAGAGCTGTTCTTGATGAGTTGCAGAAGAATCTCCAACTCAGTGAGTGGCATATGGAACCGTCTCGGATGACTCTGCATAGATTTGGAAACACTTCAAGCAGTTCTCTATGGTATGAACTGGCTTATACTGAGGCAAAGGGCCGGGTTGCAAAAGGGGATCGGGTCTGGCAGATTGCATTCGGGTCGGGCTTTAAGTGTAACAGTGCTGTGTGGAAGGCCGTGCGCGACTTGCCCGTCGTCGGGGATTGGCGGGGCAACCCCTGGGATGACTCTGTTGATAAGTATCCCGTCAGCGTCCCTGTTTCTGTTGCTTCTTAA
Protein sequence

>MS.gene22743.t1

MGRNSIDMDKERLTAEMNFKDSTSAVIKIRRRLPDFLQTVKLKYVKLGYGYSCNATTILIFTLVVPLLLFLTVQFQLTDLKLHRLSQLLLEHTVQLDTDTAIGSIVLLFLFGLYYAKRSPPIYLVDFACYKPEKERKIAVESFVKMLEDSGEFGEETLQFQRRILSRAGLGDETSLPNGIMSSPPNLCMKEARLEAEAVMFGSLDALFAKTGVNPRDIDILVVNCSLFNPTPSLSAMIVNHYKLRTNIKSYNLGGMGCSAGLISIDLAKDLLKANPNSYAVVLSTENLTLNWYFGNDRSMLLSNCIFRMGGAAILLSNKSSDRTRSKYKLVHTVRTHKGADDKNYNCVYQKEDETGKVGVSLARELMAVAGDALKTNITTLGPLVLPFSEQFMFFVSLVKRKLLLKKGSGVKPYIPDFKLAFEHFCIHAGGRAVLDELQKNLQLSEWHMEPSRMTLHRFGNTSSSSLWYELAYTEAKGRVAKGDRVWQIAFGSGFKCNSAVWKAVRDLPVVGDWRGNPWDDSVDKYPVSVPVSVAS